DOCSLIB.ORG

6174.Full-Text.Pdf

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
6174.Full-Text.Pdf Monocyte Chemoattractant Protein-1 Enhances Gene Expression and Synthesis of Matrix Metalloproteinase-1 in Human Fibroblasts by an Autocrine IL-1 α Loop This information is current as of September 30, 2021. Toshiyuki Yamamoto, Beate Eckes, Cornelia Mauch, Karin Hartmann and Thomas Krieg J Immunol 2000; 164:6174-6179; ; doi: 10.4049/jimmunol.164.12.6174 http://www.jimmunol.org/content/164/12/6174 Downloaded from References This article cites 39 articles, 18 of which you can access for free at: http://www.jimmunol.org/content/164/12/6174.full#ref-list-1 http://www.jimmunol.org/ Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication by guest on September 30, 2021 *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2000 by The American Association of Immunologists All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. Monocyte Chemoattractant Protein-1 Enhances Gene Expression and Synthesis of Matrix Metalloproteinase-1 in Human Fibroblasts by an Autocrine IL-1␣ Loop1 Toshiyuki Yamamoto,2 Beate Eckes, Cornelia Mauch, Karin Hartmann, and Thomas Krieg Monocyte chemoattractant protein-1 (MCP-1), a member of the C-C chemokine superfamily, has recently been shown to be involved in the pathogenesis of tissue fibrosis. In vitro studies demonstrated that MCP-1 up-regulates type I collagen gene expression via endogenous production of TGF-␤ in rat lung fibroblasts. We here show that recombinant human MCP-1 affects gene expression of interstitial collagenase (matrix metalloproteinase-1 (MMP-1)) in primary human skin fibroblasts and a stable fibroblast cell line. MMP-1 mRNA was induced by MCP-1 (10 ng/ml) as early as 6 h and reached a maximal expression at 24 h. MCP-1 also caused an increase of MMP-2 mRNA expression in both types of fibroblasts at 48 h. Interestingly, tissue inhibitor of Downloaded from metalloproteinase-1 (TIMP-1) mRNA was also up-regulated by MCP-1, and TIMP-1 mRNA expression peaked at 48 h in both types of fibroblasts. Immunoblot analysis demonstrated increased levels of MMP-1 and TIMP-1 protein in the culture superna- tants of primary fibroblasts stimulated with MCP-1. In addition, MCP-1 strongly induced IL-1␣ mRNA expression in dermal fibroblasts in parallel with the induction of MMP-1. Preincubation with IL-1 receptor antagonist almost completely abrogated the expression of MMP-1 mRNA, and partially inhibited MMP-1 synthesis induced by MCP-1. Transient transfection of primary skin fibroblasts with a MMP-1 promoter-reporter construct indicated a dose-dependent increase in promoter activity by MCP-1 http://www.jimmunol.org/ stimulation. These data demonstrate that MCP-1 up-regulates MMP-1 mRNA expression and synthesis in human skin fibroblasts at a transcriptional level and provide evidence that this is mediated by an IL-1␣ autocrine loop. The Journal of Immunology, 2000, 164: 6174–6179. onocyte chemoattractant protein-1 (MCP-1)3 belongs indirectly mediated by endogenous up-regulation of TGF-␤ gene to a C-C chemokine superfamily of small proteins that expression (13). M are important in recruiting and activating leukocytes Excessive deposition of connective tissue is the result of an during inflammation (1). In vitro studies have demonstrated that imbalance between synthesis and degradation of matrix constitu- numerous types of cells including fibroblasts, endothelial cells, ents. The matrix metalloproteinases (MMPs) are believed to play by guest on September 30, 2021 epithelial cells, mononuclear cells, and smooth muscle cells are a crucial role in connective tissue remodeling in a variety of phys- capable of expressing MCP-1 in the presence of serum or specific iological processes such as angiogenesis and wound healing (14, stimuli (2–7). It has also been shown that MCP-1 is up-regulated 15). MMPs are zinc-dependent enzymes that are active at neutral in human idiopathic pulmonary fibrosis (8) or in bleomycin-in- pH and cleave a variety of extracellular matrix (ECM) proteins. duced pulmonary fibrosis in rats (9, 10). Also, another C-C che- Most MMPs are secreted as a proform and are activated in close mokine, macrophage inflammatory protein-1 is supposed to play proximity to the cell surface by other active MMPs (16, 17) or by an important role in bleomycin-induced lung fibrosis (11). In ad- serine proteinases (18, 19). To maintain the normal balance of dition, a recent report indicates that MCP-1 is involved in the tissue turnover, it is important that the activity of these enzymes is formation of crescent nephritis and interstitial renal fibrosis in tightly controlled. This regulation occurs at different levels, includ- mice (12). These results provide evidence that increased MCP-1 ing transcription, activation of latent proenzymes, and inhibition of expression contributes to the development of fibrotic processes. proteolytic activity by tissue inhibitors of metalloproteinases Furthermore, a recent in vitro study has demonstrated that MCP-1 (TIMPs). Disruption of the normal control of MMPs can lead to stimulates rat lung fibroblast collagen gene expression, which is pathological consequences resulting from excessive accumulation or enhanced degradation of ECM proteins (20, 21). In this study, we have examined whether MCP-1 might contribute to the mod- ulation of ECM deposition by affecting the balance between col- Department of Dermatology, University of Cologne, Cologne, Germany lagen synthesis and gene expression of MMPs and TIMPs. Received for publication July 28, 1999. Accepted for publication March 30, 2000. The costs of publication of this article were defrayed in part by the payment of page Materials and Methods charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Cell cultures 1 This work was supported in part by a grant from the Japanese Ministry of Education. Primary normal human dermal fibroblasts, established by outgrowth from 2 Address correspondence and reprint requests to Dr. Toshiyuki Yamamoto at his skin biopsies of healthy donors as previously described (22), in passages current address: Department of Dermatology, Tokyo Medical and Dental University, 3–5, and Wi-26/SV-40 fibroblasts, a cell line originating from human em- School of Medicine, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan. E-mail bryonic lung, were used. Cells were maintained in DMEM supplemented address: [email protected] with 10% heat-inactivated FCS, 2 mM glutamine, 50 ␮g/ml sodium ascor- ␮ 3 Abbreviations used in this paper: MCP-1, monocyte chemoattractant protein-1; bate, 100 U/ml penicillin, and 100 g/ml streptomycin and grown in the ECM, extracellular matrix; IL-1ra, IL-1 receptor antagonist; MMP, matrix metallo- moist atmosphere of a CO2 incubator at 37°C. For the experiments de- proteinase; PDGF, platelet-derived growth factor; TIMP, tissue inhibitor of scribed, at least three different strains of primary fibroblasts were metalloproteinases. examined. Copyright © 2000 by The American Association of Immunologists 0022-1767/00/$02.00 The Journal of Immunology 6175 Stimulation by MCP-1 Recombinant human MCP-1 (lyophilized; R&D Systems, Minneapolis, MN) was dissolved in 0.1% BSA in PBS. After fibroblasts were grown to semiconfluence, the medium was changed to fresh DMEM without FCS. Twenty-four hours later, cells were incubated with MCP-1 at various con- centrations ranging from 1 to 50 ng/ml for time periods varying from 6 to 48 h. In a separate experiment, actinomycin D (Sigma, St. Louis, MO) (10 ␮g/ml) was added concomitantly with MCP-1 for 24 h. To examine the effect of IL-1 receptor antagonist (IL-1ra), we added IL-1ra (PeproTek, London, U.K.) (1 ␮g/ml) 2 h before stimulation with MCP-1. RNA isolation and Northern blot analysis Total RNA was isolated from monolayer cultures using RNAzol reagent (TRIZOL; Life Technologies, Grand Island, NY). Aliquots of 5 ␮g/lane were electrophoresed in denaturing agarose gels containing 0.66 M form- aldehyde, transferred to GeneScreen membranes (NEN Life Science Prod- ucts, Boston, MA), fixed by UV-cross-linking, and hybridized to cDNA probes labeled by random priming using [␣-32P]dCTP (ICN Biomedicals, Eschwege, Germany). Filters were hybridized overnight at 42°C in 50% formamide, 5ϫ SSC, 100 ␮g/ml denatured salmon sperm DNA, 5ϫ Den- hardt’s media, and 0.1% SDS, washed twice at room temperature in 2ϫ SSC, 0.1% SDS, followed by a washing step at high stringency (62–65°C Downloaded from in 0.1ϫ SSC, 0.1% SDS). Autoradiography was performed overnight at Ϫ80°C using intensifying screens (Kodak, Rochester, NY). The cDNAs used were specific for MMP-1 (PX 7) (a gift from Dr. B. Stein), MMP-2 (PBS GEL) (a gift from Dr. B. Marmer), TIMP-1 (a gift from Dr. S. Werner), MCP-1 (a gift from Dr. T. Yoshimura), IL-1␣ (23), and GAPDH (a gift from Dr. J. Uitto). Immunoblot analysis for MMP-1 http://www.jimmunol.org/ To detect MMP-1 protein synthesis induced by MCP-1, normal dermal fibroblasts were seeded at a density of 1 ϫ 106 in 100-mm diameter tissue FIGURE 1. Time course of the expression of MMP-1, MMP-2, and culture plates and stimulated with MCP-1 (10 ng/ml) for 24 h.
Load more

Recommended publications
  • Points of Strength
    -e Collagen Matrix for Hernia Repair POINTS OF STRENGTH for Hernia Repair Proven Strength Integration Lasting Repair Clinical Data Source & Processing Product Options IMPORTANCE OF STRENGTH SurgiMend® is offered in thickness of 1mm, 2mm, 3mm and 4mm. Even at 1mm thickness, SurgiMend has been shown to have the strongest uniaxial tensile strength when compared 1 to other major competitors. 30 25 20 15 10 5 Uniaxial Tensile Stress (Mpa) Tensile Uniaxial 0 Surgisis®, SurgiMend Straice™, XenMatrix™ AlloMax™ X-Thick FlexHD® Veritas® Peri-Guard® CollaMend™ CollaMend Permacol™ Biodesign® Firm AlloDerm® FM An MD Anderson study comparing mechanical properties of ADMs derived from bovine and porcine dermis found 3mm and 4mm thick SurgiMend: • To be as strong as the stainless steel suture2 Tear Resistance Thickness Maximum Load ® ™ • To haveSurgiMend twice the suture retentionStrattice of (mm), SD (N), SD porcine dermal matrix (2mm thick)2 PADM* 1.75 + 0.14 19.66† + 3.90 ** † • To be more than twice as resistant to tearing BADM 2.0 1.85 + 0.27 50.95 + 11.79 as porcine dermal matrix2 BADM 3.0 3.23 + 0.54 86.89† + 15.34 BADM 4.0 3.83 + 0.32 100.02† + 14.28 * PADM: Porcine Acellular Dermal Matrix ** BADM: Bovine Acellular Dermal Matrix † Significant difference from all other conditions by 1-way analysis of variance and Tukey post hoc analysis (P < 0.05). • SurgiMend’s increased resistance to tearing is papillary dermis related to the bovine dermal source material2,3 • Bovine and porcine dermis have significant 3 reticular dermis differences in collagen fiber weave pattern • Unlike bovine dermis, hair follicles perforate hypodermis 4 Bovine Porcine the entire dermis in porcine skin INTEGRATION & REVASCULARIZATION SurgiMend is rapidly revascularized to support tissue building and healing for prolonged reinforcement.5,6 11 ½ Months: Revascularized SurgiMend 11 ½ months after hernia repair in a patient undergoing a subsequent procedure unrelated to the original hernia repair.
    [Show full text]
  • Surgimend 1.0 2.0 3.0 4.0 Collagen Matrix for Soft Tissue Reconstruction
    ® SurgiMend 1.0 2.0 3.0 4.0 Collagen Matrix for Soft Tissue Reconstruction Bioactive, non-inflammatory, rBioactive, tive collagen non-inflammatory, matrix regenerative collagen matrix Rapidly revasculariz Rapidly revascularized and incorporated ted Product Overview SurgiMend is a unique acellular collagen matrix derived from fetal and SurgiMend for soft tissue neonatal bovine dermis. SurgiMend oers clear advantages over synthetic repair, reinforcement & and other biologic products for soft tissue repair and reconstruction. reconstruction. SurgiMend is the Strongest, Thickest Biologic Matrix • Plastic & reconstructive ● The biological make-up of bovine dermis, including its inherent surgery collagen ber architecture, leaves SurgiMend unmatched in available thicknesses and mechanical strength • Muscle flap reinforcement ● 1.0, 2.0, 3.0 & 4.0 mm thicknesses and sizes up to 25 cm x 40 cm • Hernia repair SurgiMend Offers the Highest Levels of Type III Healing Collagen ● Type III collagen mediates tissue healing while inhibiting scarring ● SurgiMend is derived from young healthy tissue that contains three The preferred choice of: times more Type III collagen than other acellular dermal matrices • General Surgeons SurgiMend is Terminally Sterilized, Safe, and Consistent • Trauma Surgeons ● Free of potentially antigenic antibiotics and terminally sterilized ● Derived from only well-dened and characterized source tissue with • Plastic & Reconstructive respect to age, mechanical strength, structure, and composition Surgeons SurgiMend is Non-Inflammatory
    [Show full text]
  • A Collagen and Silk Scaffold for Improved Healing of the Tendon
    LAB/IN VITRO RESEARCH e-ISSN 1643-3750 © Med Sci Monit, 2019; 25: 269-278 DOI: 10.12659/MSM.912038 Received: 2018.07.10 Accepted: 2018.09.25 A Collagen and Silk Scaffold for Improved Published: 2019.01.09 Healing of the Tendon and Bone Interface in a Rabbit Model Authors’ Contribution: ACDEF 1 Shengjun Qian 1 Centre for Orthopedic Research, Orthopedics Research Institute of Zhejiang Study Design A BCEF 1 Zhan Wang University, Department of Orthopedics, The Second Affiliated Hospital, School of Data Collection B Medicine, Zhejiang University, Hangzhou, Zhejiang, P.R. China Statistical Analysis C ABDF 1 Zefeng Zheng 2 Department of Orthopedics, Suichang Peoples’ Hospital, Lishui, Zhejiang, Data Interpretation D BCD 1 Jisheng Ran P.R. China Manuscript Preparation E BDF 2 Junfeng Zhu Literature Search F Funds Collection G ACDG 1 Weishan Chen Corresponding Author: Weishan Chen, e-mail: [email protected] Source of support: This work was supported by the Zhejiang Provincial Natural Science Foundation (Grant no. LQ17H060003) Background: The study aimed to develop a novel orthopedic surgical scaffold made of collagen and silk to repair the tendon and bone interface, and to investigate its influence on tendon and bone healing in a rabbit model. Material/Methods: Four types of surgical scaffold were prepared, including a random collagen scaffold (RCS), an aligned collagen scaffold (ACS), a random collagen scaffold combined with knitted silk (RCSS), and an aligned collagen scaffold combined with knitted silk (ACSS). Rabbit bone marrow stem cells (BMSCs) were cultured and seeded onto the RCS and ACS scaffold. The animal model included four-month-old female New Zealand White rabbits (N=20) that underwent drilling into the rotator cuff of the left supraspinatus muscle tendon, randomized into the ACSS and RCSS groups.
    [Show full text]
  • Application of Purified Porcine Collagen in Patients with Chronic
    Korean J Pain 2020;33(4):395-399 https://doi.org/10.3344/kjp.2020.33.4.395 pISSN 2005-9159 eISSN 2093-0569 Clinical Research Article Application of purified porcine collagen in patients with chronic refractory musculoskeletal pain Hyunyoung Seong, Raing Kyu Kim, Youngjae Shin, Hye Won Lee, and Jae Chul Koh Department of Anesthesiology and Pain Medicine, Korea University Anam Hospital, Seoul, Korea Received April 27, 2020 Revised August 7, 2020 Background: This study aimed to assess the potential efficacy of purified porcine Accepted August 17, 2020 atelocollagen (PAC) for the management of refractory chronic pain due to suspected connective tissue damage. Handling Editor: Joon-Ho Lee Methods: Patients treated with PAC were retrospectively evaluated. Patients with chronic refractory pain, suspected to have originated from musculoskeletal damage Correspondence or defects with the evidence of imaging studies were included. Pain intensity, using Jae Chul Koh the 11-point numerical rating scale (NRS), was assessed before the procedure, and Department of Anesthesiology and 1 month after the last procedure. Pain Medicine, Korea University Anam Results: Eighty-eight patients were finally included for investigation. The mean NRS Hospital, 73 Goryeodae-ro, Seongbuk-gu, Seoul 02841, Korea score was decreased from 5.8 to 4.1 after 1 month of PAC injection (P < 0.001). No Tel: +82-2-920-5632 independent factor was reported to be directly related to the decrease in NRS score Fax: +82-2-920-5630 by more than half. E-mail: [email protected] Conclusions: Application of PAC may have potential as a treatment option for re- fractory chronic musculoskeletal pain.
    [Show full text]
  • No.7 July 2004
    Vol.47, No.7 July 2004 CONTENTS JMA Policies Policy Address Haruo UEMATSU . 305 Regenerative Medicine Regenerative Medicine for Cartilage Defects Mitsuo OCHI . 307 The Use of Skin Regeneration Technique in the Treatment of Full-Thickness Skin Defects Norio KUMAGAI . 311 Regenerative Medicine for the Cornea Shigeru KINOSHITA and Takahiro NAKAMURA . 317 Regenerative Medicine for Sclerotic Disorders Toshikazu NAKAMURA . 322 Regenerative Medicine for Cardiomyocytes Keiichi FUKUDA . 328 Regenerative Medicine for Respiratory Diseases Tatsuo NAKAMURA . 333 Centenarians Implications of Research Findings Obtained from Centenarians Hiroshi SHIBATA . 338 Abdominal Aortic Aneurysm Forefront of Treatment for Abdominal Aortic Aneurysm Hiroshi YASUHARA . 344 ⅥJMA Policies Policy Address JMAJ 47(7): 305–306, 2004 Haruo UEMATSU President, Japan Medical Association The 110th General Assembly of the JMA House of Delegates was held for two days from April 1 to 2 at the JMA Hall. Elections for the offices of the executive board were held on the first day and Dr. Haruo Uematsu (former president of the Osaka Medical Association) was elected as the 16th president of the JMA for the first time. The follow- ing is a main part of the policy address of Dr. Uematsu presented on April 2, 2004. Our country is facing a period of great tional women, to foster their capabilities, and changes as can be seen in the dispatch of Self- to send them to the JMA in future. Defense Forces to Iraq by the government of Since this is such an important time for the Japan amidst an ongoing debate about revising JMA, I believe that making the organization’s the Japanese Constitution.
    [Show full text]
  • Qt9661q7w2.Pdf
    UCLA UCLA Previously Published Works Title The Role of the Anti-Inflammatory Cytokine Interleukin-10 in Tissue Fibrosis. Permalink https://escholarship.org/uc/item/9661q7w2 Journal Advances in wound care, 9(4) ISSN 2162-1918 Authors Steen, Emily H Wang, Xinyi Balaji, Swathi et al. Publication Date 2020-04-01 DOI 10.1089/wound.2019.1032 Peer reviewed eScholarship.org Powered by the California Digital Library University of California COMPREHENSIVE INVITED REVIEW The Role of the Anti-Inflammatory Cytokine Interleukin-10 in Tissue Fibrosis Emily H. Steen,1,2 Xinyi Wang,2 Swathi Balaji,2 Manish J. Butte,3 Paul L. Bollyky,4 and Sundeep G. Keswani1,2,5,* 1Department of Surgery, Baylor College of Medicine, Houston, Texas. 2Laboratory for Regenerative Tissue Repair, Texas Children’s Hospital, Houston, Texas. 3Division of Immunology, Allergy, and Rheumatology, Department of Pediatrics, University of California, Los Angeles, Los Angeles, California. 4Division of Infectious Diseases, Department of Medicine, Stanford University School of Medicine, Stanford, California. 5Division of Pediatric Surgery, Department of Surgery, Texas Children’s Hospital, Houston, Texas. Significance: Fibrosis is the endpoint of chronic disease in multiple organs, including the skin, heart, lungs, intestine, liver, and kidneys. Pathologic accu- mulation of fibrotic tissue results in a loss of structural integrity and function, with resultant increases in morbidity and mortality. Understanding the path- ways governing fibrosis and identifying therapeutic targets within those path- ways is necessary to develop novel antifibrotic therapies for fibrotic disease. Recent Advances: Given the connection between inflammation and fibrogenesis, Interleukin-10 (IL-10) has been a focus of potential antifibrotic therapies because of its well-known role as an anti-inflammatory mediator.
    [Show full text]
  • Tendon Proper- and Peritenon-Derived Progenitor Cells Have Unique Tenogenic Properties Michael J Mienaltowski1,2,3*, Sheila M Adams1 and David E Birk1,2
    Mienaltowski et al. Stem Cell Research & Therapy 2014, 5:86 http://stemcellres.com/content/5/4/86 RESEARCH Open Access Tendon proper- and peritenon-derived progenitor cells have unique tenogenic properties Michael J Mienaltowski1,2,3*, Sheila M Adams1 and David E Birk1,2 Abstract Introduction: Multipotent progenitor populations exist within the tendon proper and peritenon of the Achilles tendon. Progenitor populations derived from the tendon proper and peritenon are enriched with distinct cell types that are distinguished by expression of markers of tendon and vascular or pericyte origins, respectively. The objective of this study was to discern the unique tenogenic properties of tendon proper- and peritenon-derived progenitors within an in vitro model. We hypothesized that progenitors from each region contribute differently to tendon formation; thus, when incorporated into a regenerative model, progenitors from each region will respond uniquely. Moreover, we hypothesized that cell populations like progenitors were capable of stimulating tenogenic differentiation, so we generated conditioned media from these cell types to analyze their stimulatory potentials. Methods: Isolated progenitors were seeded within fibrinogen/thrombin gel-based constructs with or without supplementation with recombinant growth/differentiation factor-5 (GDF5). Early and late in culture, gene expression of differentiation markers and matrix assembly genes was analyzed. Tendon construct ultrastructure was also compared after 45 days. Moreover, conditioned media from tendon proper-derived progenitors, peritenon-derived progenitors, or tenocytes was applied to each of the three cell types to determine paracrine stimulatory effects of the factors secreted from each of the respective cell types. Results: The cell orientation, extracellular domain and fibril organization of constructs were comparable to embryonic tendon.
    [Show full text]
  • Tendon Tissue Engineering: Progress, Challenges, and Translation to the Clinic
    J Musculoskelet Neuronal Interact 2011; 11(2):163-173 Review Article Hylonome Tendon tissue engineering: Progress, challenges, and translation to the clinic J.T. Shearn1, K.R.C. Kinneberg1, N.A. Dyment1, M.T. Galloway2, K. Kenter3, C. Wylie4, D.L. Butler1 1Orthopaedic Tissue Engineering and Biomechanics Laboratory; Biomedical Engineering Program; School of Energy, Environment, Biological and Medical Engineering; College of Engineering and Applied Science; University of Cincinnati; 2Cincinnati Sportsmedicine and Orthopaedic Center; 3Department of Orthopaedic Surgery; College of Medicine; University of Cincinnati; 4Division of Developmental Biology; Cincinnati Children’s Hospital Medical Center, USA Abstract The tissue engineering field has made great strides in understanding how different aspects of tissue engineered constructs (TECs) and the culture process affect final tendon repair. However, there remain significant challenges in developing strategies that will lead to a clinically effective and commercially successful product. In an effort to increase repair quality, a better under- standing of normal development, and how it differs from adult tendon healing, may provide strategies to improve tissue engi- neering. As tendon tissue engineering continues to improve, the field needs to employ more clinically relevant models of tendon injury such as degenerative tendons. We need to translate successes to larger animal models to begin exploring the clinical im- plications of our treatments. By advancing the models used to validate our TECs, we can help convince our toughest customer, the surgeon, that our products will be clinically efficacious. As we address these challenges in musculoskeletal tissue engineering, the field still needs to address the commercialization of products developed in the laboratory.
    [Show full text]
  • Surgimend® 1.0 2.0 3.0 4.0 Collagen Matrix for Soft Tissue Reconstruction
    Product Summary ® SurgiMend 1.0 2.0 3.0 4.0 Collagen Matrix for Soft Tissue Reconstruction Because we are committed to limiting uncertainty, Integra offers SurgiMend in 1, 2, 3 or 4 mm thicknesses and in over sixty configurations, providing the most appropriate device thickness, strength, and size for each procedure, technique, and patient. SurgiMend® 1.0 2.0 3.0 4.0 Collagen Matrix for Soft Tissue Reconstruction Product Overview SurgiMend® is a unique acellular collagen matrix derived from fetal and neonatal bovine dermis. SurgiMend offers clear advantages over synthetic and other biologic products for soft tissue repair and reconstruction. SurgiMend is the Strongest, Thickest Biologic Matrix 9 • The biological make-up of bovine dermis, including its inherent collagen fiber architecture, leaves SurgiMend unmatched in available thicknesses and mechanical strength • 1.0, 2.0, 3.0 and 4.0 mm thicknesses and sizes up to 25 cm x 40 cm SurgiMend Offers an Abundance of Type III Healing Collagen 3,4 • Type III collagen mediates tissue healing while inhibiting scarring • SurgiMend is derived from young healthy tissue that contains three times more Type III collagen than other acellular dermal matrices SurgiMend is Terminally Sterilized, Safe, and Consistent • Free of potentially antigenic antibiotics and terminally sterilized • Derived from only well-defined and characterized source tissue with respect to age, mechanical strength, structure, and composition SurgiMend is Non-Inflammatory for Better Reinforcement • Does not elicit an acute or chronic
    [Show full text]
  • In Vitro Differentiation of Adipose-Derived Mesenchymal Stem Cell Into Insulin-Producing Cells
    OPEN ACCESS http://scidoc.org/IJST.php International Journal of Stem Cell Research and Transplantation (IJST) ISSN: 2328-3548 In vitro Differentiation of Adipose-Derived Mesenchymal Stem Cell into Insulin-Producing Cells Research Article Babiker NE1, Gassoum A2, Musa HH1, Hamed ALDeaf SA2, Abdelraheem NE2,3, Fadl-Elmula I6, Ali El-Sheikh MA4, Abdelrahman Arbab M2,5* 1 Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan. 2 National Center of Neurological Sciences, Khartoum, Sudan. 3 Faculty of Medical Laboratory Sciences, National University, Khartoum, Sudan. 4 Faculty of Medicine, University of Khartoum, Khartoum, Sudan. 5 Faculty of Medicine, Department of Surgery, University of Khartoum, Khartoum, Sudan. 6 Faculty of Medicine, Al Neelain University, Khartoum, Sudan. Abstract Adipose or fatty tissue is similar to bone marrow ontogenetically. Mesenchymal stem cells can be isolated from different types of adipose tissue depots in greater amount than other sources, making them especially suitable for use in regenerative medicine. Adipose tissue was taken from Sudanese donors; the SVF which contains MSCs was isolated using enzymatic and density centrifugation techniques. The AD-MSCs were differentiated to insulin producing cells using three steps protocol. The therapeutic effect of islet β-like cells was determined in vivo using diabetics albino Wister rats. The adherent cells firstly appeared round and spherical in shape, the characteristic shape of MSC was detected after three weeks incubation. The pheno type of these cells showed positives CD34 and CD13 and negatives CD45 and HLADR markers. AD-MSCs were induced into insulin producing cells by a 3-step (15-days) protocol. The differentiated cells were positive for diathizone stain and displayed positive immuno-reactivity to antihuman insulin antibody.
    [Show full text]
  • UNIVERSITY of WISCONSIN-LA CROSSE Graduate Studies
    UNIVERSITY OF WISCONSIN-LA CROSSE Graduate Studies INCREASE OF EXTRACELLULAR MATRIX PROTEINS IN HIBERNATING GROUND SQUIRRELS COULD HELP MAINTAIN BONE HEALTH A Manuscript Style Thesis Submitted in Partial Fulfillment of the Requirements for the Degree of Master of Science – Biology: Physiology Concentration Hannah Bergen College of Science and Health Biology: Physiology Concentration December, 2018 ABSTRACT Bergen, H.R. Increase of extracellular matrix proteins in hibernating ground squirrels could help maintain bone health. MS in Physiology, December 2018, pp.43. (S. Cooper) Whether from injury, old age, or space flight, immobility or a lack of gravitational loading has negative effects on the physiology of bone and bone marrow. Consequences include a weakening of the immune system, decreased hematopoietic cells in the bone marrow, and an overall decrease in bone density. Understanding the mechanisms behind these effects and combating their side effects is a subject of intense research. Thirteen lined ground squirrels are a common model organism for these studies because they hibernate for months and experience long periods of inactivity. Like humans under low loading and activity, the ground squirrels experience changes in the bone marrow cell make up and decreased bone density, but, unlike humans, once a 13-lined ground squirrel becomes active again, the side effects from their long rest are minute or non-existent. Transcriptomes were constructed from genes expressed in bone marrow from hibernating and non-hibernating ground squirrels. Certain extracellular matrix proteins, including COL4A2, were seen to increase drastically during hibernation and may possibly play a role in maintaining the health of the bone. iii ACKNOWLEDGEMENTS I want to thank my dad and mom, for shaping me into who I am today, for believing in me and supporting me, not just through school but each step and decision life presents.
    [Show full text]
  • Trix Consisting of Citric Acid and Collagen
    Advance Online Publication © Annals of Vascular Diseases 2011 doi:10.3400/avd.oa.11.00017 Original Article Enhanced Neovascular Formation in a Novel Hydrogel Ma- trix Consisting of Citric Acid and Collagen Mikiko Nagayoshi, MD,1, 2 Tetsushi Taguchi, PhD,3 Hiroyuki Koyama, MD,1, 2 Tsuyoshi Takato, MD,2 Tetsuro Miyata, MD,1 and Hirokazu Nagawa, MD1 Background: Three-dimensional regenerative tissue with large bulk generally requires blood perfusion through a vascular network to maintain its viability, and one promising approach is induction of neovascu- lar growth from the recipient bed into the tissue. To induce ingrowth of a vascular network, it is necessary to furnish the regenerative tissue with a scaffold structure for neovasculature and a delivery system for an angiogenic growth factor. As such a scaffold structure, the present study created novel hydrogel materials by chemically cross-linking alkali-treated collagen (AlCol) with trisuccinimidyl citrate (TSC). Materials and Methods: Many prototypes, consisting of several concentrations of TSC and AlCol, were implanted into the subfascial space of the rat rectus muscle, and 7 days later, the implanted materials were excised for histological analysis. Cross-sections were stained and neovascular development in the materials was evaluated by measuring vessel density, length and number of joints and branches. Results: Significant ingrowth of vascularized granulation was observed in some materials, which surpassed the angiogenic ability of MatrigelTM. Further, combination with basic fibroblast growth factor (bFGF) sig- nificantly increased the vascular formation in these gels. Conclusions: The TSC-AlCol gel functioned as a favorable scaffold for neovascular formation and also as a reservoir for controlled delivery of bFGF.
    [Show full text]