POLR2L Antibody Cat

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POLR2L Antibody Cat. No.: XW-7445 POLR2L Antibody Specifications HOST SPECIES: Chicken SPECIES REACTIVITY: Human, Mouse, Rat IMMUNOGEN: 1-67 TESTED APPLICATIONS: WB POLR2L antibody can be used for the detection of POLR2L by Western blot, may also work APPLICATIONS: for IHC and ICC. PREDICTED MOLECULAR 7.6 kDa (calculated) WEIGHT: Properties PURIFICATION: Antigen affinity-purified CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Phosphate-Buffered Saline. No preservatives added. CONCENTRATION: 1 mg/mL October 1, 2021 1 https://www.prosci-inc.com/polr2l-antibody-7445.html POLR2L antibody can be stored at 4˚C for short term (weeks). Long term storage should STORAGE CONDITIONS: be at -20˚C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: POLR2L DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA ALTERNATE NAMES: polymerase III subunit L, RNA polymerases I, and III subunit ABC5, RBP10, RPB10, RPABC5, RPB7.6, hRPB7.6, RPB10beta, POLR2L ACCESSION NO.: NP_066951.1 PROTEIN GI NO.: 10863925 GENE ID: 5441 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References DNA directed RNA polymerase II polypeptide L; polymerase (RNA) II (DNA directed) polypeptide L (7.6kD); RNA polymerase II subunit. This protein is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. BACKGROUND: It contains four conserved cysteines characteristic of an atypical zinc-binding domain. Like its counterpart in yeast, this subunit may be shared by the other two DNA-directed RNA polymerases. 1) Cramer, P. et al. Architecture of RNA polymerase II and implications for the REFERENCES: transcription mechanism. Science 288 (5466), 640-649 (2000). ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 1, 2021 2 https://www.prosci-inc.com/polr2l-antibody-7445.html.

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Molecular Pharmacology of Cancer Therapy in Human Colorectal Cancer by Gene Expression Profiling1,2
[CANCER RESEARCH 63, 6855–6863, October 15, 2003] Molecular Pharmacology of Cancer Therapy in Human Colorectal Cancer by Gene Expression Profiling1,2 Paul A. Clarke,3 Mark L. George, Sandra Easdale, David Cunningham, R. Ian Swift, Mark E. Hill, Diana M. Tait, and Paul Workman Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey SM2 5NG [P. A. C., M. L. G., S. E., P. W.]; Department of Gastrointestinal Oncology, Royal Marsden Hospital, Sutton, Surrey [D. C., M. E. H., D. M. T.]; and Department of Surgery, Mayday Hospital, Croydon, Surrey [M. L. G., R. I. S.], United Kingdom ABSTRACT ment with a single dose of MMC4 and during a continuous infusion of 5FU. In this study, we report for the first time gene expression Global gene expression profiling has potential for elucidating the com- profiling in cancer patients before, and critically, during the period of plex cellular effects and mechanisms of action of novel targeted anticancer exposure to chemotherapy. We have demonstrated that the approach agents or existing chemotherapeutics for which the precise molecular is feasible, and we have detected a novel molecular response that mechanism of action may be unclear. In this study, decreased expression would not have been predicted from in vitro studies and that would of genes required for RNA and protein synthesis, and for metabolism were have otherwise been missed by conventional approaches. The results detected in rectal cancer biopsies taken from patients during a 5-fluorou- also suggest a possible new therapeutic approach. Overall our obser- racil infusion. Our observations demonstrate that this approach is feasible and can detect responses that may have otherwise been missed by con- vations suggest that gene expression profiling in response to treatment ventional methods.
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POLR2L Is Over-Expressed in Human Endometrial Cancer
Over-expression of RNA polymerase II subunit L in human endometrial cancer. Shahan Mamoor, MS1 [email protected] East Islip, NY USA Gynecologic cancers including cancers of the endometrium are a clinical problem1-4. We mined published microarray data5,6 to discover genes associated with endometrial cancers by comparing transcriptomes of the normal endometrium and endometrial tumors from humans. We identified RNA polymerase II subunit L, encoded by POLR2L, as among the most differentially expressed genes, transcriptome-wide, in cancers of the endometrium. POLR2L was expressed at significantly higher levels in endometrial tumor tissues as compared to the endometrium. Importantly, primary tumor expression of POLR2L was correlated with recurrence-free survival in patients with endometrial cancer. POLR2L may be a molecule of interest in understanding the etiology or progression of human endometrial cancer. Keywords: endometrial cancer, gynecologic cancers, endometrium, POLR2L, RNA polymerase II subunit L, systems biology of endometrial cancer, targeted therapeutics in endometrial cancer. 1 Endometrial cancer is the most common gynecologic cancer in the developed world1. Over the last three decades, the incidence of endometrial cancer has increased 21%4 and the death rate has increased 100%3. We harnessed the power of independently published microarray datasets5,6 to determine in an unbiased fashion and at the systems-level genes most differentially expressed in endometrial tumors. We report here the differential and increased expression of the RNA polymerase II subunit L (POLR2L) in human endometrial cancer. Methods We utilized datasets GSE636785 and GSE1158106 for this global differential gene expression analysis of human endometrial cancer in conjunction with GEO2R.
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Anti-POLR2L (GW22520F)
3050 Spruce Street, Saint Louis, MO 63103 USA Tel: (800) 521-8956 (314) 771-5765 Fax: (800) 325-5052 (314) 771-5757 email: [email protected] Product Information Anti-POLR2L antibody produced in chicken, affinity isolated antibody Catalog Number GW22520F Formerly listed as GenWay Catalog Number 15-288-22520F, DNA-directed RNA polymerase II 7.6 kDa polypeptide Antibody. The product is a clear, colorless solution in phosphate – Storage Temperature Store at 20 °C buffered saline, pH 7.2, containing 0.02% sodium azide. Synonyms: DNA directed RNA polymerase II polypeptide L, Species Reactivity: Human, mouse EC 2.7.7.6; RPB10; RPB7.6; RPABC5 Tested Applications: WB Product Description Recommended Dilutions: Recommended starting dilution DNA-dependent RNA polymerase catalyzes the transcrip- for Western blot analysis is 1:500, for tissue or cell staining tion of DNA into RNA using the four ribonucleoside 1:200. triphosphates as substrates. Note: Optimal concentrations and conditions for each NCBI Accession number: NP_066951.1 application should be determined by the user. Swiss Prot Accession number: P62875 Precautions and Disclaimer Gene Information: Human .. POLR2L (5441) This product is for R&D use only, not for drug, household, or Immunogen: Recombinant protein DNA directed RNA other uses. Due to the sodium azide content a material polymerase II polypeptide L safety data sheet (MSDS) for this product has been sent to the attention of the safety officer of your institution. Please Immunogen Sequence: GI # 10863925, sequence 1 - 67 consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. Storage/Stability For continuous use, store at 2–8 °C for up to one week.
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