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Identification of Medicinal Plants Within the Apocynaceae Family Using ITS2 and Psba-Trnh Barcodes
Available online at www.sciencedirect.com Chinese Journal of Natural Medicines 2020, 18(8): 594-605 doi: 10.1016/S1875-5364(20)30071-6 •Special topic• Identification of medicinal plants within the Apocynaceae family using ITS2 and psbA-trnH barcodes LV Ya-Na1, 2Δ, YANG Chun-Yong1, 2Δ, SHI Lin-Chun3, 4, ZHANG Zhong-Lian1, 2, XU An-Shun1, 2, ZHANG Li-Xia1, 2, 4, LI Xue-Lan1, 2, 4, LI Hai-Tao1, 2, 4* 1 Yunnan Branch, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical Col- lege, Jinghong 666100, China; 2 Key Laborartory of Dai and Southern Medicine of Xishuangbanna Dai Autonomous Prefecture, Jinghong 666100, China; 3 Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People’s Re- public of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical Col- lege, Beijing, 100193, China; 4 Engineering Research Center of Tradition Chinese Medicine Resource, Ministry of Education, Institute of Medicinal Plant De- velopment, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China Available online 20 Aug., 2020 [ABSTRACT] To ensure the safety of medications, it is vital to accurately authenticate species of the Apocynaceae family, which is rich in poisonous medicinal plants. We identified Apocynaceae species by using nuclear internal transcribed spacer 2 (ITS2) and psbA- trnH based on experimental data. The identification ability of ITS2 and psbA-trnH was assessed using specific genetic divergence, BLAST1, and neighbor-joining trees. For DNA barcoding, ITS2 and psbA-trnH regions of 122 plant samples of 31 species from 19 genera in the Apocynaceae family were amplified. -
Floral Extracts of Allamanda Blanchetii and Allamanda Cathartica Are Comparatively Higher Resource of Anti-Oxidants and Polysaccharides Than Leaf and Stem Extracts
International Journal of Current Pharmaceutical Research ISSN- 0975-7066 Vol 10, Issue 4, 2018 Original Article FLORAL EXTRACTS OF ALLAMANDA BLANCHETII AND ALLAMANDA CATHARTICA ARE COMPARATIVELY HIGHER RESOURCE OF ANTI-OXIDANTS AND POLYSACCHARIDES THAN LEAF AND STEM EXTRACTS CHANDREYI GHOSH, SAYANTAN BANERJEE Department of Biotechnology, Techno India University, West Bengal, EM-4, Sector V, Salt Lake, Kolkata 7000091 Email: [email protected] Received: 21 Apr 2018, Revised and Accepted: 10 Jun 2018 ABSTRACT Objective: The present study undertakes a comparative analysis of the level of secondary metabolites present in the leaf, flower and stem of the two ornamental plants, Allamanda blanchetii and Allamanda cathartica. Methods: The two plant species, Allamanda blanchetii and Allamanda cathartica were collected, washed, shade dried in room temperature and powered in mechanical grinder. Phytochemicals were extracted from the power with methanol and double distilled water. The estimation of flavonoids, polyphenols, polysaccharide were done by standard methods and the anti-oxidant activity was measured by 1,1-diphenyl-2- picrylhydrazyl (DPPH) discoloration assay. Results: Our study reveals that the flower of both species contain highest amount of secondary metabolites in crude methanolic and aqueous extracts. In case of leaf, the methanolic extracts contain higher amount of polyphenol, flavonoid and anti-oxidant property in comparison to aqueous extracts, where as the aqueous extract contain higher amount of polysaccharide content than its counterpart. In stem, crude organic extract has higher amount of polyphenol and flavonoid and the aqueous extract has higher amount of polysaccharide and anti-oxidant property. Conclusion: The flower of Allamanda cathartica and Allamanda blanchetii has higher amount of flavonoids, polyphenols, polysaccharide and the floral extracts display comparatively higher anti-oxidant property. -
Phytochemical & Biological Evaluation of Cascabela Thevetia with Special
. Presented by: Biman Bhuyan Assistant Professor Department of Pharmaceutical Sciences Dibrugarh University Dibrugarh-786004, Assam, India Email ID:[email protected] Presented at: XIXth International Congress "Phytopharm 2015“ New Phytotherapeutics – Developments, Requirements and Success for Patients with Rational Phytotherapy. (Institute of Pharmacy, University of Bonn; July 21-24, 2015 ) . Diabetes mellitus is chronic disorder of carbohydrate, protein and fat metabolism resulting from insulin deficiency and abnormality in the use of insulin. Due to several problems with available conventional therapies, alternative approaches become essential to treat diabetes. Plant based medicine has become one of the most promising alternative strategy for treatment of diabetes. In this study the bark of Cascabela thevetia L. was selected for evaluation of antidiabetic activity based on its traditional use in Assam. T. Miyagawa et al. (J. Nat. Prod., 2009) in his article “Cardenolide Glycosides of Thevetia peruviana and Triterpenoid Saponins of Sapindus emarginatus as TRAIL Resistance-Overcoming Compounds” reported that cardenolide glycosides isolated from Thevetia peruviana (Cascabela thevetia) have significant reversal effect on TRAIL resistance in human gastric adrenocarcinoma cells. M.H. Khan et al. (Indian J Tradit Know., 2010) in his article “Antidiabetic plants used in Thoubal district of Manipur, North-East India” reported that bark of the plant is used by Meitei community for treatment of diabetes. V. Bandara et al. (Toxicon, 2010) in his article “A review of the natural history, toxinology, diagnosis and clinical management of Nerium oleander (common oleander) and Thevetia peruviana (yellow oleander) poisoning” reported the cardiac glycosides related toxicity and their clinical management in emergency. J. Buragohain (Recent Res. -
Survey of Roadside Alien Plants in Hawai`I Volcanoes National Park and Adjacent Residential Areas 2001–2005
Technical Report HCSU-032 SURVEY OF ROADSIDE ALIEN PLANts IN HAWAI`I VOLCANOES NATIONAL PARK AND ADJACENT RESIDENTIAL AREAS 2001–2005 Linda W. Pratt1 Keali`i F. Bio2 James D. Jacobi1 1 U.S. Geological Survey, Pacific Island Ecosystems Research Center, Kilauea Field Station, P.O. Box 44, Hawaii National Park, HI 96718 2 Hawai‘i Cooperative Studies Unit, University of Hawai‘i at Hilo, P.O. Box 44, Hawai‘i National Park, HI 96718 Hawai‘i Cooperative Studies Unit University of Hawai‘i at Hilo 200 W. Kawili St. Hilo, HI 96720 (808) 933-0706 September 2012 This product was prepared under Cooperative Agreement CA03WRAG0036 for the Pacific Island Ecosystems Research Center of the U.S. Geological Survey. Technical Report HCSU-032 SURVEY OF ROADSIDE ALIEN PLANTS IN HAWAI`I VOLCANOES NATIONAL PARK AND ADJACENT RESIDENTIAL AREAS 2001–2005 1 2 1 LINDA W. PRATT , KEALI`I F. BIO , AND JAMES D. JACOBI 1 U.S. Geological Survey, Pacific Island Ecosystems Research Center, Kīlauea Field Station, P.O. Box 44, Hawai`i Volcanoes National Park, HI 96718 2 Hawaii Cooperative Studies Unit, University of Hawai`i at Hilo, Hilo, HI 96720 Hawai`i Cooperative Studies Unit University of Hawai`i at Hilo 200 W. Kawili St. Hilo, HI 96720 (808) 933-0706 September 2012 This article has been peer reviewed and approved for publication consistent with USGS Fundamental Science Practices ( http://pubs.usgs.gov/circ/1367/ ). Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. -
ORNAMENTAL GARDEN PLANTS of the GUIANAS: an Historical Perspective of Selected Garden Plants from Guyana, Surinam and French Guiana
f ORNAMENTAL GARDEN PLANTS OF THE GUIANAS: An Historical Perspective of Selected Garden Plants from Guyana, Surinam and French Guiana Vf•-L - - •• -> 3H. .. h’ - — - ' - - V ' " " - 1« 7-. .. -JZ = IS^ X : TST~ .isf *“**2-rt * * , ' . / * 1 f f r m f l r l. Robert A. DeFilipps D e p a r t m e n t o f B o t a n y Smithsonian Institution, Washington, D.C. \ 1 9 9 2 ORNAMENTAL GARDEN PLANTS OF THE GUIANAS Table of Contents I. Map of the Guianas II. Introduction 1 III. Basic Bibliography 14 IV. Acknowledgements 17 V. Maps of Guyana, Surinam and French Guiana VI. Ornamental Garden Plants of the Guianas Gymnosperms 19 Dicotyledons 24 Monocotyledons 205 VII. Title Page, Maps and Plates Credits 319 VIII. Illustration Credits 321 IX. Common Names Index 345 X. Scientific Names Index 353 XI. Endpiece ORNAMENTAL GARDEN PLANTS OF THE GUIANAS Introduction I. Historical Setting of the Guianan Plant Heritage The Guianas are embedded high in the green shoulder of northern South America, an area once known as the "Wild Coast". They are the only non-Latin American countries in South America, and are situated just north of the Equator in a configuration with the Amazon River of Brazil to the south and the Orinoco River of Venezuela to the west. The three Guianas comprise, from west to east, the countries of Guyana (area: 83,000 square miles; capital: Georgetown), Surinam (area: 63, 037 square miles; capital: Paramaribo) and French Guiana (area: 34, 740 square miles; capital: Cayenne). Perhaps the earliest physical contact between Europeans and the present-day Guianas occurred in 1500 when the Spanish navigator Vincente Yanez Pinzon, after discovering the Amazon River, sailed northwest and entered the Oyapock River, which is now the eastern boundary of French Guiana. -
European Academic Research
EUROPEAN ACADEMIC RESEARCH Vol. IV, Issue 10/ January 2017 Impact Factor: 3.4546 (UIF) ISSN 2286-4822 DRJI Value: 5.9 (B+) www.euacademic.org Evidences from morphological investigations supporting APGIII and APGIV Classification of the family Apocynaceae Juss., nom. cons IKRAM MADANI Department of Botany, Faculty of Science University of Khartoum, Sudan LAYALY IBRAHIM ALI Faculty of Science, University Shandi EL BUSHRA EL SHEIKH EL NUR Department of Botany, Faculty of Science University of Khartoum, Sudan Abstract: Apocynaceae have traditionally been divided into into two subfamilies, the Plumerioideae and the Apocynoideae. Recently, based on molecular data, classification of Apocynaceae has undergone considerable revisions. According to the Angiosperm Phylogeny Group III (APGIII, 2009), and the update of the Angiosperm Phylogeny Group APG (APGIV, 2016) the family Asclepiadaceae is now included in the Apocynaceae. The family, as currently recognized, includes some 1500 species divided in about 424 genera and five subfamilies: Apocynoideae, Rauvolfioideae, Asclepiadoideae, Periplocoideae, and Secamonoideae. In this research selected species from the previous families Asclepiadaceae and Apocynaceae were morphologically investigated in an attempt to distinguish morphological important characters supporting their new molecular classification. 40 morphological characters were treated as variables and analyzed for cluster of average linkage between groups using the statistical package SPSS 16.0. Resulting dendrograms confirm the relationships between species from the previous families on the basis of their flowers, fruits, 8259 Ikram Madani, Layaly Ibrahim Ali, El Bushra El Sheikh El Nur- Evidences from morphological investigations supporting APGIII and APGIV. Classification of the family Apocynaceae Juss., nom. cons and seeds morphology. Close relationships were reported between species from the same subfamilies. -
Allamanda Cathartica Linn. Apocynaceae: a Mini Review
International Journal of Herbal Medicine 2019; 7(4):29-33 E-ISSN: 2321-2187 P-ISSN: 2394-0514 IJHM 2019; 7(4): 29-33 Allamanda cathartica Linn. Apocynaceae: A mini Received: 10-05-2019 Accepted: 14-06-2019 review Chandreyi Ghosh Department of Biotechnology, Chandreyi Ghosh, Labani Hazra, Sudip Kumar Nag, Sayantan Sil, Techno India University, Kolkata, West Bengal, India Alolika Dutta, Swagata Biswas, Maitrayee Biswas, Pranabesh Ghosh and Sirshendu Chatterjee Labani Hazra Department of Biotechnology, Techno India University, Abstract Kolkata, West Bengal, India Allamanda cathartica Linn. (Family –Apocynaceae) is a perennial shrub, found in various parts of the world. The common name of the plant is Golden Trumpet flower, and in Bengali, it is known as Sudip Kumar Nag Harkakra. The plant is also known to deal with heat and different toxic products; it activates blood Department of Biotechnology, circulation and diuresis. It works well against snake bite. In traditional medicinal practices, the plant is Techno India University, used to cure skin infection, cold and cough, and various other inflammations. The plant possesses various Kolkata, West Bengal, India secondary metabolite substances like flavonoids, polyphenols, iridoids, tannins, and alkaloids. Various pharmacological studies concluded some notable bioactivities of the plant such as anti-inflammatory, Sayantan Sil anti-microbial, wound healing, etc. This review aims to explain the overviews of the various uses and Department of Biotechnology, prospects as well as agricultural, taxonomical, phytochemical, pharmacological, and toxicological areas Techno India University, of the Allamanda cathartica. Kolkata, West Bengal, India Alolika Dutta Keywords: Allamanda cathartica, Harkakra, traditional medicine, phytopharmacology Department of Biotechnology, Techno India University, Introduction Kolkata, West Bengal, India Allamanda cathartica Linn. -
Phylogeny and Systematics of the Rauvolfioideae
PHYLOGENY AND SYSTEMATICS Andre´ O. Simo˜es,2 Tatyana Livshultz,3 Elena OF THE RAUVOLFIOIDEAE Conti,2 and Mary E. Endress2 (APOCYNACEAE) BASED ON MOLECULAR AND MORPHOLOGICAL EVIDENCE1 ABSTRACT To elucidate deeper relationships within Rauvolfioideae (Apocynaceae), a phylogenetic analysis was conducted using sequences from five DNA regions of the chloroplast genome (matK, rbcL, rpl16 intron, rps16 intron, and 39 trnK intron), as well as morphology. Bayesian and parsimony analyses were performed on sequences from 50 taxa of Rauvolfioideae and 16 taxa from Apocynoideae. Neither subfamily is monophyletic, Rauvolfioideae because it is a grade and Apocynoideae because the subfamilies Periplocoideae, Secamonoideae, and Asclepiadoideae nest within it. In addition, three of the nine currently recognized tribes of Rauvolfioideae (Alstonieae, Melodineae, and Vinceae) are polyphyletic. We discuss morphological characters and identify pervasive homoplasy, particularly among fruit and seed characters previously used to delimit tribes in Rauvolfioideae, as the major source of incongruence between traditional classifications and our phylogenetic results. Based on our phylogeny, simple style-heads, syncarpous ovaries, indehiscent fruits, and winged seeds have evolved in parallel numerous times. A revised classification is offered for the subfamily, its tribes, and inclusive genera. Key words: Apocynaceae, classification, homoplasy, molecular phylogenetics, morphology, Rauvolfioideae, system- atics. During the past decade, phylogenetic studies, (Civeyrel et al., 1998; Civeyrel & Rowe, 2001; Liede especially those employing molecular data, have et al., 2002a, b; Rapini et al., 2003; Meve & Liede, significantly improved our understanding of higher- 2002, 2004; Verhoeven et al., 2003; Liede & Meve, level relationships within Apocynaceae s.l., leading to 2004; Liede-Schumann et al., 2005). the recognition of this family as a strongly supported Despite significant insights gained from studies clade composed of the traditional Apocynaceae s. -
Floral Glands in Asclepiads: Structure, Diversity and Evolution
Acta Botanica Brasilica - 31(3): 477-502. July-September 2017. doi: 10.1590/0102-33062016abb0432 Review Floral glands in asclepiads: structure, diversity and evolution Diego Demarco1 Received: December 7, 2016 Accepted: February 24, 2017 . ABSTRACT Species of Apocynaceae stand out among angiosperms in having very complex fl owers, especially those of asclepiads, which belong to the most derived subfamily (Asclepiadoideae). Th ese fl owers are known to represent the highest degree of fl oral synorganization of the eudicots, and are comparable only to orchids. Th is morphological complexity may also be understood by observing their glands. Asclepiads have several protective and nuptial secretory structures. Th eir highly specifi c and specialized pollination systems are associated with the great diversity of glands found in their fl owers. Th is review gathers data regarding all types of fl oral glands described for asclepiads and adds three new types (glandular trichome, secretory idioblast and obturator), for a total of 13 types of glands. Some of the species reported here may have dozens of glands of up to 11 types on a single fl ower, corresponding to the largest diversity of glands recorded to date for a single structure. Keywords: anatomy, Apocynaceae, Asclepiadoideae, diversity, evolution, fl ower, secretory structures considering its most derived subfamily Asclepiadoideae. Introduction Th e close relationship between the former families Apocynaceae and Asclepiadaceae has always been recognized Apocynaceae is an extremely diverse family in since its establishment as “Apocineae” by Jussieu (1789). morphological terms, represented by trees, shrubs, herbs and climbers, with single leaves usually opposite, rarely Although Brown (1810) divided it into two families and alternate or whorled, with stipules modifi ed in colleters in this separation had been maintained in the subsequent several species (Endress & Bruyns 2000; Capelli et al. -
Thevetia Peruviana- a Review on Its Characteristic Features and Toxic Constituents S Rajhans#1, J
International Journal of Scientific Research and Review ISSN NO: 2279-543X Thevetia peruviana- A Review on Its Characteristic Features and Toxic Constituents S Rajhans#1, J. Pandya#2A. U. Mankad#3 and H. A. Pandya#3 Department of Botany, Gujarat University, Ahmedabad-380009 Abstract- Thevetia peruviana (Pers.) K. Schum. belongs to the Apocynaceae family. It is found throughout the tropical and sub-tropical regions of the world. The plant is best known as an ornamental. It is also known for its cardiotoxic properties. This paper has made an attempt in presenting a comprehensive review of the plant based on its origin, botanical description, distribution, growing requirements characteristic features and its toxic constituents. Keywords- Thevetia peruviana (Pers.) K. Schum., evergreen, survival, cardiac glycosides and toxins Introduction- Thevetia peruviana (Pers.) K. Schum. is an evergreen shrub belonging to the Apocynaceae family. Native to Mexico, South and Central America this plant is now naturally found in the tropical and sub-tropical regions of the world. Commonly known as the yellow oleander in English and as Peeli Kaner in Hindi this plant is easily found in different parts of India. The plant is grown mainly as an ornamental in the gardens, road dividers and on road sides. It does not require any kind of maintenance and can easily survive in extreme conditions. The characteristic features of this plant is its milky sap. It is also known for its toxic parts and the various contents of toxins in them. SYNONYMS- Thevetia neriifoliia A. Juss. Ex Steud; Cerbera thevetia L; Cascabela thevetia (L.) Lippold. HABIT- A straight (erect) and abundantly branched shrub usually growing from 2.5m to 3.5m tall but rarely a small tree reaching a height up to 10 m in height. -
ISSN: 2320-5407 Int. J. Adv. Res. 5(8), 486-493 RESEARCH ARTICLE
ISSN: 2320-5407 Int. J. Adv. Res. 5(8), 486-493 Journal Homepage: - www.journalijar.com Article DOI: 10.21474/IJAR01/5081 DOI URL: http://dx.doi.org/10.21474/IJAR01/5081 RESEARCH ARTICLE THEVETIA PERUVIANA: A MULTIPURPOSE MEDICINAL PLANT- A REVIEW. *Tabrez Ahmad1, Abdulhamid Tahir Hamid2, Anuradha Sharma1 and Uma Bhardwaj3. 1. Department of Biotechnology, School of Life and Applied Sciences, Baba Farid Institute of Technology, Dehradun, Uttarakhand, India. 2. School of Biotechnology, Maharaj Vinayak Global University, Jaipur, Rajasthan, India. 3. Dean Research, Swami Rama Himalayan University, Dehradun, Uttarakhand, India. …………………………………………………………………………………………………….... Manuscript Info Abstract ……………………. ……………………………………………………………… Manuscript History Plant based therapeutics play an important role in the public health care system of any nation. The plant Thevetia peruviana belongs to the Received: 05 June 2017 family Apocynaceae. The plant have a significant place in traditional Final Accepted: 07 July 2017 system of medicine of Central and South America and tropical Asia. A Published: August 2017 number of different classes of secondary metabolites are present in Key words:- Thevetia peruviana including alkaloids, flavonoids, steroids, cardiac Thevetia peruviana, Apocynaceae, glycosides, terpenoids, tannins, saponins etc. Several researchers had Cardiac glycosides, identified various pharmacological activities in different parts of the Ethanopharmacology plant viz. seeds, flowers, bark, fruits, leaves. This review covers detailed ethanopharmacology, toxicology and bioactivities of Thevetia peruviana. Copy Right, IJAR, 2017,. All rights reserved. …………………………………………………………………………………………………….... Introduction:- Our planet is home for enormous medicinal plants. Mankind use of medicinal plants is not new rather dates back to thousands of years. In fact, ancient man was very dependent on green plants for his day-to-day needs of medicaments. -
UNIVERSIDADE ESTADUAL DE CAMPINAS Instituto De Biologia
UNIVERSIDADE ESTADUAL DE CAMPINAS Instituto de Biologia TIAGO PEREIRA RIBEIRO DA GLORIA COMO A VARIAÇÃO NO NÚMERO CROMOSSÔMICO PODE INDICAR RELAÇÕES EVOLUTIVAS ENTRE A CAATINGA, O CERRADO E A MATA ATLÂNTICA? CAMPINAS 2020 TIAGO PEREIRA RIBEIRO DA GLORIA COMO A VARIAÇÃO NO NÚMERO CROMOSSÔMICO PODE INDICAR RELAÇÕES EVOLUTIVAS ENTRE A CAATINGA, O CERRADO E A MATA ATLÂNTICA? Dissertação apresentada ao Instituto de Biologia da Universidade Estadual de Campinas como parte dos requisitos exigidos para a obtenção do título de Mestre em Biologia Vegetal. Orientador: Prof. Dr. Fernando Roberto Martins ESTE ARQUIVO DIGITAL CORRESPONDE À VERSÃO FINAL DA DISSERTAÇÃO/TESE DEFENDIDA PELO ALUNO TIAGO PEREIRA RIBEIRO DA GLORIA E ORIENTADA PELO PROF. DR. FERNANDO ROBERTO MARTINS. CAMPINAS 2020 Ficha catalográfica Universidade Estadual de Campinas Biblioteca do Instituto de Biologia Mara Janaina de Oliveira - CRB 8/6972 Gloria, Tiago Pereira Ribeiro da, 1988- G514c GloComo a variação no número cromossômico pode indicar relações evolutivas entre a Caatinga, o Cerrado e a Mata Atlântica? / Tiago Pereira Ribeiro da Gloria. – Campinas, SP : [s.n.], 2020. GloOrientador: Fernando Roberto Martins. GloDissertação (mestrado) – Universidade Estadual de Campinas, Instituto de Biologia. Glo1. Evolução. 2. Florestas secas. 3. Florestas tropicais. 4. Poliploide. 5. Ploidia. I. Martins, Fernando Roberto, 1949-. II. Universidade Estadual de Campinas. Instituto de Biologia. III. Título. Informações para Biblioteca Digital Título em outro idioma: How can chromosome number