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MONOCLONAL ANTIBODY to MOUSE CD71, TRANSFERRIN RECEPTOR 1 (TFRC) Clone ER-MP21

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MONOCLONAL ANTIBODY to MOUSE CD71, TRANSFERRIN RECEPTOR 1 (TFRC) Clone ER-MP21 MONOCLONAL ANTIBODY TO MOUSE CD71, TRANSFERRIN RECEPTOR 1 (TFRC) clone ER-MP21 Catalog no HM1085 (lot number and expiry date are indicated on the label) Description The monoclonal antibody ER-MP21 recognizes CD71, the transferrin receptor 1. CD71 is a 200 kDa glycoprotein composed of two identical, disulfide-linked chains. Each chain is capable of binding transferrin, an iron-transport molecule. Binding of transferrin to its receptor followed by endocytosis of the receptor-ligand complex is a major cellular iron-uptake mechanism. Iron uptake by the proliferating cell is essential for the iron-containing enzyme ribonucleotide reductase involved with DNA synthesis. CD71 is not only expressed by cycling cells, but also by cells that require iron for other iron-dependent proteins, such as the enzyme peroxidase in monocytes. Rapidly proliferating cells, as in malignancy, generally express CD71 abundantly. Furthermore, CD71 has been described as a marker of immature, proliferating T cells. The monoclonal antibody ER-MP21 inhibits proliferation and differentiation during early T cell development. ER-MP21 recognizes murine CD71, but does not compete with transferrin binding. Aliases TFRC, TFR1, T9, p90 Species Rat IgG2a Formulation 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum Application F FC FS IA IF IP P W Yes ● ● ●a No ● N.D. ● ● ● ● a = Inhibition of biological activity N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot Use For immunohistology, and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting. Storage and Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year stability Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product. References 1. Leenen, P et al; Differential inhibition of macrophage proliferation by anti-transferrin receptor antibody ER-MP21: correlation to macrophage differentiation stage. Exp Cell Res 1990, 189: 55 2. Brekelmans, P et al; Transferrin receptor expression as a marker of immature cycling thymocytes in the mouse. Cell Immunol 1994, 159: 331 3. Brekelmans, P et al; Inhibition of proliferation and differentiation during early T cell development by anti-transferrin receptor antibody. Eur J Immunol 1994, 24: 2896 Also available HM1082 Monoclonal antibody against Mouse Ly-6C, clone ER-MP20 HM1083 Monoclonal antibody against Mouse CD13, clone ER-BDMD1 HM1084 Monoclonal antibody against Mouse PECAM-1 (CD31), clone ER-MP12 HM1015 Monoclonal antibody against Mouse CD34, clone MEC14.7 HM1066 Monoclonal antibody against Mouse F4/80-Macrophages, clone BM8 Website: www.hycultbiotech.com India Contact: Life Technologies (India) Pvt. Ltd. 306, Aggarwal City Mall, Opposite M2K Pitampura, Delhi – 110034 (INDIA). Ph: +91-11-42208000, 42208111, 42208222, Mobile: +91-9810521400, Fax: +91-11-42208444 Email: [email protected] Website: www.lifetechindia.com.
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