Uva-DARE (Digital Academic Repository)

UvA-DARE (Digital Academic Repository)

The bright and DARC side of detection Role of membrane proteins during erythroid maturation Aglialoro, F.

Publication date 2020 Document Version Other version License Other Link to publication

Citation for published version (APA): Aglialoro, F. (2020). The bright and DARC side of detection: Role of membrane proteins during erythroid maturation.

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Download date:01 Oct 2021

Francesca Aglialoro Francesca Role of membrane proteins of Role during erythroid maturation The bright and DARC side of detection: side of and DARC The bright

The bright and DARC side of detection: Role of membrane proteins during erythroid maturation Francesca Aglialoro The bright and DARC side of detection: Role of membrane proteins during erythroid maturation

Francesca Aglialoro Printing was supported by Sanquin Research

ISBN: 9789464190151

Layout: Giuseppe Lo Giudice and Francesca Aglialoro

Cover design: Francesca Aglialoro

Printed by: Gildeprint The bright and DARC side of detection: Role of membrane proteins during erythroid maturation

ACADEMISCH PROEFSCHRIFT

ter verkrijging van de graad van doctor aan de Universiteit van Amsterdam op gezag van de Rector Magnificus prof. dr. ir. K.I.J. Maex ten overstaan van een door het College voor Promoties ingestelde commissie, in het openbaar te verdedigen in de Agnietenkapel op vrijdag 6 november 2020, te 10.00 uur

door

Francesca Aglialoro geboren te Palermo Promotiecommissie

Promotor: dr. M.M. von Lindern AMC-UvA

Copromotor dr. E. van den Akker Sanquin Research

Overige leden: prof. dr. C.E. van der Schoot AMC-UvA

prof. dr. M.J. Kersten AMC-UvA

prof. dr. M. de Haas Universiteit Leiden

prof. dr. J.J. Voorberg AMC-UvA

dr. H.A. van Wijk UMC Utrecht

prof. dr. L. Kaestner Universität des Saarlandes

Faculteit der Geneeskunde “Life begins at the end of your comfort zone” N.D. Walsch

Contents

Chapter 1 General introduction 13

Chapter 2 Mechanical stress mediated Ca2+ induced signal transduction in 50 erythroblasts modulates erythropoiesis

Chapter 3 Inside out integrin activation mediated by PIEZO1 signaling in 76 erythroblasts

Chapter 4 DARC extracellular domain remodeling in maturating 100 reticulocytes explains Plasmodium vivax tropism

Chapter 5 Towards long term continuous Plasmodium vivax blood stage 116 cultures using in vitro cultured reticulocytes

Chapter 6 Differential interaction between DARC and SDF-1 on erythrocytes 146 and their precursors

Chapter 7 General discussion 165

Appendix Summary 182 Samenvatting Sommario List of publications PhD portfolio Curriculum Vitae Acknowledgements Abbreviation List

Acronym Full name AQP1 Aquaporin 1 BCAM Basal Cell Adhesion Molecule BMP-4 Bone Morphogenetic Protein 4 CAR Contractile Actomyosine Ring CD147 Basignin Cluster of differentiation; transmembrane CD34 phosphoglycoprotein CD44 Cell surface glycoprotein CD44 CD47 Integrin Associated Protein CD49d Integrin alpha subunit CD71 Transferrin Receptor 1 (Trf1) CRIK Citron Rho-interacting Kinase EMP Erythroblast Macrophage Protein EPO Erythropoietin Endosomal sorting complexes required for ESCRT transport Gas6 Growth arrest-specific 6 GLUT4 Glucose transporter type 4 Gnc5 General control nonderepressible 5 GPA Glycophorin A GPC Glycophorin C HAT Histone Acetyl Transferase HDAC Histone Deacetylase HIV Human Immunodeficiency Virus ICAM-4 Intracellular Adhesion Molecule 4 IL-6 Interleukin 6 INF-gamma Interferon gamma KCC Potassium Chloride Cotransporter LC3 Light chain 3b LPA Lysophosphatidic Acid Lu Lutheran LW Landsteiner-Wiener MDS Myelodysplastic syndrome Proto-oncogene tyrosine-protein kinase MERTK MER MLC2 Myosin regulatory Light Chain 2 MVB Multivesicular Body MVEs Multivesicular Endosomes PBMC peripheral blood mononuclear cells Phosphatidylinositol-4,5-bisphosphate 3-ki- PI3K nase PKA Protein Kinase A PKB Protein Kinase B Rab11 Ras-related protein RBC Red blood cells Rh Rhesus RhAG Rh-Associated Glycoprotein Rho-GTPase Rho-GuanosineTriPhosphate SCD Sickle Cell Disease SCF Stem Cell Factor TAM-receptor Tyrosine Activation Motif receptor TNF-alpha Tumor Necrosis Factor alpha TO Thiazole Orange VCAM1 Vascular Cell Adhesion Molecule 1 Xk Kell blood group precursor