Monoclonal Antibody to TNFRSF21 / Death Receptor 6 (DR6) - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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SM3132P Monoclonal Antibody to TNFRSF21 / Death receptor 6 (DR6) - Purified

Alternate names: Tumor necrosis factor receptor superfamily member 21 Quantity: 0.1 mg Concentration: 1.0 mg/ml Background: CD358, also called Death Receptor-6, is a type I transmembrane protein of the TNF receptor superfamily which is able of inducing apoptosis through its cytoplasmic death domain. DR6 serves as an important regulator for CD4+ T cell proliferation and Th differentiation. It provides also a regulatory mechanism for B cell activation and humoral immune responses. Uniprot ID: O75509 NCBI: NP_055267.1 GeneID: 27242 Host / Isotype: Mouse / IgG1 Recommended Isotype SM10P (for use in human samples), AM03095PU-N Controls: Clone: DR-6-04-EC Immunogen: A fusion protein representing amino acids 42-335 (extracellular part) of Human DR6 linked to the Fc portion of Human IgG1. Format: State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Purification: Affinity Chromatography on Protein A Buffer System: PBS, pH~7.4 containing 15 mM Sodium Azide as preservative Applications: Flow Cytometry: 1 µg/ml. Immunocytochemistry. Immunoprecipitation: Antibody immunoprecipitates overexpressed DR6 from DR6-transfected HEK/293 cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: The antibody clone DR-6-04-EC recognizes DR6 (Death receptor-6), widely expressed in most human tissues and cell lines. Species Reactivity: Tested: Human. Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Pan G, Bauer JH, Haridas V, Wang S, Liu D, Yu G, et al. Identification and functional characterization of DR6, a novel death domain-containing TNF receptor. FEBS Lett. 1998 Jul 24;431(3):351-6. PubMed PMID: 9714541. 2. Kasof GM, Lu JJ, Liu D, Speer B, Mongan KN, Gomes BC, et al. Tumor necrosis factor- alpha induces the expression of DR6, a member of the TNF receptor family, through

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. CS/20150331 1 / 2 SM3132P: Monoclonal Antibody to TNFRSF21 / Death receptor 6 (DR6) - Purified

activation of NF-kappaB. Oncogene. 2001 Nov 29;20(55):7965-75. PubMed PMID: 11753679. 3. Rossi D, Gaidano G. Messengers of cell death: apoptotic signaling in health and disease. Haematologica. 2003 Feb;88(2):212-8. PubMed PMID: 12604411. 4. Schmidt CS, Liu J, Zhang T, Song HY, Sandusky G, Mintze K, et al. Enhanced B cell expansion, survival, and humoral responses by targeting death receptor 6. J Exp Med. 2003 Jan 6;197(1):51-62. PubMed PMID: 12515813. Pictures: Detection of DR6 transiently transfected into HeLa cells (green) using anti-DR6 antibody (DR-6-04-EC). Cell nuclei visualized by DAPI (blue).

Flow cytometry analysis of cell surface expression of DR6 in 6 different cell lines using purified anti-DR6 antibody (DR-6-04-EC) (detection by Goat anti- mouse IgG1 PE).

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. CS/20150331 2 / 2