Сравнительное Описание Апотециев Видов Evernia Mesomorpha И Evernia Esorediosa

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Сравнительное Описание Апотециев Видов Evernia Mesomorpha И Evernia Esorediosa Бюллетень Ботанического сада-института ДВО РАН, 2018. Вып. 19. С. 65–68. doi: 10.17581/bbgi1908 Сравнительное описание апотециев видов Evernia mesomorpha и Evernia esorediosa © Т.М. Харпухаева ФГБУН Институт общей и экспериментальной биологии СО РАН, г. Улан-Удэ, Россия e-mail: [email protected] Evernia mesomorpha – широко распространенный в Северном полушарии ли- шайник, размножающийся преимущественно вегетативным путем. В 2015 г. в окрестностях пос. Байкит (Красноярский край) были собраны образцы с развитыми апотециями. Приводится описание строения апотециев, спор, вто- ричных метаболитов в сравнении с близкородственным видом E. esorediosa. Ключевые слова: Evernia mesomorpha, Evernia esorediosa, апотеции Evernia mesomorpha Nyl. и E. esorediosa (Müll. хроматографии (Orange, 2001). Использовалась Arg.) Du Rietz в бореальной зоне встречаются прак- вытяжка в ацетоне из кусочков таллома. Брались тически на всех породах деревьев, предпочитая од- по 2 образца от каждого вида из разных мест про- нако хвойные виды (Харпухаева, 2010). Идентифи- израстания. Применялись пластины для высоко- кация стерильных образцов вида E. mesomorpha не эффективной хроматографии «Sorbfil» производ- вызывает затруднений, поскольку апотеции у него ства ООО «Имид» и 2 системы растворителей: А крайне редки, их строение не было описано в от- (толуол : диоксан : ледяная уксусная кислота) и С ечественной литературе. (толуол : ледяная уксусная кислота). Анатомиче- Вид Evernia mesomorpha широко распростра- ские особенности апотециев изучены на образцах нен в Северном полушарии, является массовым в E. mesomorpha из Красноярского края (3 образца), бореальной зоне, в Арктике редок. На севере дохо- и на образцах E. esorediosa из Бурятии и Краснояр- дит до Гренландии, на юге до Индии и провинции ского края (6 образцов). Юннань (Китай), но там находки вида приурочены к высокогорьям с умеренным климатом (Evernia Результаты и обсуждение mesomorpha, iNaturalist database, 2017). Встреча- Evernia mesomorpha. Таллом мягкий, повиса- ется по всей России (Urbanavichus, 2010). Evernia ющий до 10 см длиной, зеленовато-сероватый до esorediosa встречается только в Азии – от Урала грязно-желтовато-зеленоватого. Лопасти радиаль- до Охотского моря, на юге заходит в Монголию, ные, угловато-округлые, в местах ветвления упло- на юго-востоке – северные области Китая и Япо- щенные, до 3–4 мм шир. Соредии точковидные, ния (Evernia esorediosa, iNaturalist database, 2017). многочисленные, часто покрывают всю поверх- В России: Восточная Сибирь (Красноярский край, ность лопастей. Изидии короткие, палочковидные, Якутия, Иркутская обл., Бурятия, Читинская обл.), простые или разветвленные, сероватые. Апотеции Дальний Восток (Urbanavichus, 2010). развиваются редко, блюдцевидные с коричневым диском. В литературе размер спор указывается для Материалы и методы Фенноскандии и запада США – 8–9 х 5–6 и 8 μm Формы E. mesomorpha с апотециями обнару- (Ahti et al., 2011; Nash et al., 2002). жены на ветвях лиственницы и ели в лиственнич- Evernia esorediosa. Таллом кустистый, пови- нике голубично-зеленомошном в окрестностях сающий, 6–11 см длиной, 9–12 (15) см шириной, пос. Байкит (Красноярский край) (Байкитский хр, бледно-зеленовато-желтоватый. Лопасти 1,5–2 мм левобережье р. Подкаменная Тунгуска, выс. 180 м шириной, угловато-радиальные, в местах ветвления над ур. м., 61º39´56,6´´N 96º26´06,5´´E). В этом же уплощенные, до 4 мм шириной, дихотомически или районе обычным является вид E. esorediosa. неопределенно разветвленные, продольно-лакуноз- Изучение анатомического строения проводи- ные, на концах постепенно к концам утончающиеся, лось на световом микроскопе. Анализ вторичных обычно с оттянутыми, слабо разветвленными, реже метаболитов выполнен методами тонкослойной коротко разветвленными кончиками. Апотеции мно- © Ботанический сад-институт ДВО РАН. 2018 65 Харпухаева гочисленные, 2–15 мм в диаметре, округлые с тем- экземпляров E. mesomorpha крупнее, чем приводи- но-коричневым блестящим диском, сначала вогну- мые для Европы и Северной Америки (Ahti et l., тым, позднее плоским диском, окруженным тонким 2011; Nash et al, 2002). Сравнение их с апотециями слоевищным краем. Размер спор не приводится. E. esorediosa показало сходство: размеры апотеци- Н.С. Голубкова (Golubkova, 1983), считала, что ев и их анатомия близки (Рис.). Споры у E. esoredi- эти два вида – дериваты тургайской флоры и состав- osa мельче, чем у E. mesomorpha, несмотря на то, ляют пару, из которой – E. mesomorpha более моло- что размер апотециев у E. esorediosa крупнее. дой. Вегетативное размножение дало последнему возможность широко расселиться по Голарктике. Список литературы Тонкослойная хроматография не показала Ahti T., Moberg R.; Thell A. (eds.) 2011. Parmeliaceae. In: различий в химическом составе у стерильных и Nordic Lichen Flora. Vol. 4. Uppsala. 242 pp. фертильных форм в образцах E. mesomorpha (Бу- Evernia mesomorpha Nyl. In: iNaturalist database. рятия, Красноярский край) с фертильными образ- Available at: https://www.inaturalist.org/observa- tions/5100571 (accessed 22.05.2017) цами E. esorediosa (Бурятия, Красноярский край). Evernia esorediosa (Müll. Arg.) Du Rietz. In: iNatural- Во всех образцах обнаружены 2 вещества: уснино- ist database. Available at: http://www.discoverlife. вая и диварикатовая кислоты и следы атранорина. org/20/m?kind=Evernia+esorediosa&b=EOL/pag- В литературных источниках как основные приво- es/2858685 (accessed 22.05.2017) дятся усниновая и диварикатовая кислоты (Ahti et [Golubkova] Голубкова Н.С. 1983. Анализ флоры ли- l., 2011; Nash et al., 2002), а также 2 неидентифици- шайников Монголии. Л. 248 с. рованных вещества и атранорин (Golubkova, 1996). [Golubkova] Голубкова Н.С. 1996. Род Evernia. В кн.: Разделить виды методом тонкослойной хромато- Определитель лишайников России. Вып. 6. СПб. С. графии не удалось, поэтому различия нужно искать 49–56. на морфологическом уровне. [Kharpukhaeva] Харпухаева Т.М. 2010. Лишайни- ки Джергинского государственного природного Заключение заповедника. Улан-Удэ. 156 с. Evernia mesomorpha размножается преиму- Nash T.H., Ryan B.D., Gries C., Bungartz F. (eds.) 2002. Lichen Flora of the Greater Sonoran Desert Region. Vol щественно вегетативным путем, но в оптималь- 1. Tempe. 532 pp. ных условиях переходит к более древнему по- Orange A., James P.W. and White F.J. 2001. Microchemical ловому способу размножения. Впервые описано Methods for the Identification of Lichens. London. 101 строение апотеция и спор у образцов Evernia me- pp. somorpha (Табл., рис.). Также приведен размер спор [Urbanavichus] Урбанавичюс Г.П. 2010. Список для Evernia esorediosa. Размеры спор у изученных лихенофлоры России. СПб. 194 с. Таблица. Сравнительное описание апотециев Evernia mesomorpha и Evernia esorediosa Table. A comparative description of the apothecia of Evernia mesomorpha and Evernia esorediosa Evernia mesomorpha Evernia esorediosa Апотеции редки, леканоровые, окружены слоевищным краем, часто Апотеции многочисленные, леканоровые, боковые, сидячие окру- соредиозным, одного цвета со слоевищем. Молодые апотеции глубоко жены слоевищным краем одного цвета со слоевищем. Молодые вогнутые, от 1,5 до 5 мм в диаметре. С возрастом становятся более апотеции глубоко вогнутые, от 1,5 до 12 мм в диаметре. С возрастом плоскими – 6–9 мм. Диск красно-коричневый блестящий. становятся слабо вогнутыми, до плоских – 12–17 мм. Диск красно- Apothecia are rare, lecanorine, surrounded by a thallus margin, often sore- коричневый, блестящий. diate, the same color as a thallus. Young apothecia deeply concave, from Apothecia numerous, lecanorine, lateral, sessile, surrounded by a margin 1.5 to 5 mm in diameter. With age, they become more flat – 6–9 mm. The concolorous with thallus. Young apothecia deeply concave, from 1.5 to 12 disc is red-brown, shiny. mm in diameter. With age, they become slightly concave to flat – 12–17 mm. The disc is red-brown, shiny. Эпитеций коричневый, гимений бесцветный 40 μm, гипотеций Эпитеций коричневый, гимений бесцветный 31,2–36,5 μm, гипотеций бесцветный 13–15 μm. Собственный край развит. Гимений и сумки бесцветный 13–18 μm. Собственный край развит. Гимений и сумки дают реакцию с КJ + синий. дают реакцию с КJ + синий. The epithecium is brown, the hymenium is colorless 40 μm, the hypoth- The epithecium is brown, the hymenium is colorless 31.2–36.5 μm, the ecium is colorless 13–15 μm. Own margin is developed. Hymenium and hypothecium is colorless 13–18 μm. Own margin is developed. Hymenium asci give a reaction with KJ + blue. and asci give a reaction with KJ + blue. Сумки булавовидные, 39х13 μm, Lecanora-, Parmelia-типа, с толусом. Сумки булавовидные, 39х13 μm, Lecanora-, Parmelia-типа, с толусом. В сумке по 8 спор. В сумке по 8 спор. Asci are clavate, 39x13 μm, Lecanora-, Parmelia-type, with a tolus. Ascus Asci are clavate, 39x13 μm, Lecanora-, Parmelia-type, with a tolus. Ascus of 8 spores. of 8 spores. Споры широкоэллипсоидные, бесцветные, одноклеточные, от (7,6) Споры широкоэллипсоидные бесцветные, одноклеточные, от (6,4) 9,2 до 10,4 х 5,2 μm. 7,6 х 3,9–5,2 μm. Spores are broadly ellipsoidal, colorless, unicellular, from (7.6) 9.2 to 10.4 Spores are broadly ellipsoidal, colorless, unicellular, from (6.4) 7.6 x x 5.2 μm. 3.9–5.2 μm. 66 Бюллетень Ботанического сада-института ДВО РАН. 2018 Вып. 19 Сравнительное описание апотециев видов Evernia mesomorpha и Evernia esorediosa A-1) A-2) B-1) B-2) C-1) C-2) D-1) D-2) Рисунок. Анатомическое строение апотециев Evernia mesomorpha и Evernia esorediosa (ориг.) Evernia mesomorpha: A-1) – апотеции; B-1) – гимений; C-1) – сумки; D-1) – споры Evernia
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  • Bulletin of The
    Bulletin of the Califqrnia Lichen Society Volume 6 No. 2 Winter 1999 The California Lichen Society seeks to promote the appreciation, conservation , and study of t he li chens . The interests of the Society include the entire western part of the continent, although the principal focus is on California. Dues are $18 per year ( $20 for foreign subscribers) payable to The Cal ifornia Lichen Society, 362 Scenic Ave., Santa Rosa , CA, 95407. Members receive the Bulletin and notices of meetings, field trips , and workshops. Board Members of the California Lichen Society: President: Judy Robertson Vice President: Darrell Wright Secretary: Charis Bratt Treasurer: Bill Hill Member at Large: Richard Moe The Bulletin of the California Lichen Society (ISSN 1 093-9148) is edited by Isabelle Tavares, Shirley Tucker, William Sanders, Richard Moe, and Darrell Wright and is produced by Richard Moe. The Bulletin welcomes manuscripts on technical topics in lichenology relating to western North America and on conservation of the lichens, as well as news of lichenologists and their activities . Manuscripts may be submitted to Richard Moe, Bulletin of the California Lichen Society, University Herbarium, 1001 Valley Life Sciences Bldg . #2465, University of California, Berkeley, CA 94720-2465. The best way to submit manuscripts apart from short articles and announcements is by E-mail or on diskette in WordPerfect or Microsoft Word format; ASCI I format is a very good alternative. Manuscripts should be double-spaced. Figures are the usual line drawings and sharp black and white glossy photos, unmounted, and must be sent by surface mail. A review process is followed.
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  • A New Species, Sagediopsis Vasilyevae, and Other Lichenicolous Fungi from Zabaikal’Skii Territory of Russia, Southern Siberia
    Folia Cryptog. Estonica, Fasc. 51: 121–130 (2014) http://dx.doi.org/10.12697/fce.2014.51.14 A new species, Sagediopsis vasilyevae, and other lichenicolous fungi from Zabaikal’skii Territory of Russia, southern Siberia Mikhail P. Zhurbenko1 & Lidiya S. Yakovchenko2 1Laboratory of the Systematics and Geography of Fungi, Komarov Botanical Institute, Russian Academy of Sciences, Professora Popova str., 2, St. Petersburg, 197376, Russia. E-mail: [email protected] 2 Laboratory of Lower Plants, Institute of Biology and Soil Science, Far Eastern Branch of Russian Academy of Sciences, Stoletija Vladivostoka Avenue, 159, Vladivostok, 690022, Russia; Laboratory of Cryptogamic Biota, Botanical Garden- Institute Far Eastern Branch of Russian Academy of Sciences, Makovskogo str., 142, Vladivostok, 690024, Russia. E-mail: [email protected] Abstract: Thirty seven species of lichenicolous fungi are reported from southern Siberia. Sagediopsis vasilyevae (on Rhizocarpon inarense) is described as new to science. Rosellinula haplospora is new to Russia. Abrothallus peyritschii, Arthonia apotheciorum and Lichenostigma cosmopolites are new to Siberia. Cetraria is a new host genus for Stigmidium microcarpum. Ameroconium cladoniae and Plectocarpon hypogymniae are newly documented on Cladonia alaskana and Hypogymnia tubulosa correspondingly. INTRODUCTION Lichenicolous fungi of Siberia are comparatively ferential interference contrast (DIC) optics. well studied only in its arctic part (Zhurbenko, Microscopical examination was done in water, 2007, 2009a,b).
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  • The Chemistry of Smellable Molecules → Volatility?
    The chemistry of smellable molecules volatility? The chemical categories to which most odoriferous substances belong are : 1. Terpenes (= isoprenoides): e.g. Menthol, lemonene, thymol 2. Phenoles e.g. Coumarin, vanillin, heliotropin 3. Sulfuric compounds: e.g. Allyl isothiocyanate = volatile mustard oil 4. Amines e.g. Methylamine (dead fish) 5. Aldehydes e.g. Acetaldehyde (ripe fruits) benzaldehyde (bitter almond) 6. Esters & Lactones (cyclic esters) e.g. Allyl amyl glycolate (pineapple) 1. We smell only short molecules, large ones are no longer volatile and hence not odorous. 2. If molecule is very short, short is also its endurance, it will be a top note like e.g. dimethyl sulfide CSC – the transient smell of truffles 3. If a molecules carry a large net charges they will stick to each other and form hydrogen bonds. This prevents volatility. 4. Most olfactory molecules are made of C, H, O, N, S Perfumes – art, witchcraft or science? Perfumes are mixtures of raw materials: fragrant essential oils and other odorants, musk, fixatives, chemicals + solvents to give the human body, food, detergent, soap, juice, books etc pleasant smells. Why perfumes? Extracts from plants do not really smell like the real flowers but like a weak fake which might resemble but never match nature & original. The perfume maker therefore combines various raw materials to get something that resembles real flowers , or may even create some desirable fantasy smell The first perfume that used pure chemicals was in 1881Fougere Royal or Royal Fern by Parquet: it used lots of coumarin. Coumarin was used before but it was extracted from woodruff, vanilla leaf, & Tonka beans ($ 450/ kg), but not the pure, cheap chemical ($ 10/kg).
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  • Annotated Checklists of Bryophytes and Lichens Cape Prince of Wales, Alaska, U.S.A
    Annotated Checklists of Bryophytes and Lichens Cape Prince of Wales, Alaska, U.S.A. by JoAnn W. Flock, Museum Associate, University of Colorado Herbarium Version 1, May 10, 2011 Cape Prince of Wales, from Cape Mountain Photo by Tass Kelso Location of the study area on the tip of the Seward Peninsula on the west coast of Alaska. CONTENTS INTRODUCTION .......................................................................................................................... 2 DESCRIPTION OF THE STUDY AREA ..................................................................................... 3 ANNOTATED CHECKLIST OF BRYOPHYTES ....................................................................... 4 MOSSES ..................................................................................................................................... 4 LIVERWORTS ......................................................................................................................... 14 ANNOTATED CHECKLIST OF LICHENS ............................................................................... 16 REFERENCES TO LICHEN COLLECTIONS AND EXPEDITIONS IN ALASKA- CANADA ................................................................................................................................. 29 ACKNOWLEDGEMENTS .......................................................................................................... 30 INTRODUCTION In June 1978, I had the good fortune to spend two weeks at Cape Prince of Wales on the western tip of Alaska's Seward Peninsula
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  • Lichen Bioindicators for Mercury Contamination
    Lichen bioindicators for mercury contamination Rotary Kiln, New Almaden Quicksilver County Park DTMC Meeting – May 19, 2021 Peter Weiss-Penzias, Faculty Researcher Department of Microbiology and Environmental Toxicology UC Santa Cruz [email protected] Outline • Utility of lichen as a bioindicator of atmospheric mercury deposition. • Need for monitoring with lichen to identify deposition hotspots. • Recent results in Hg-mine impacted areas. • Calibrating lichen Hg with direct measurements of Hg dry deposition. • Application of method to the Carson River Superfund site. Ramalina farinaceae Flavopunctelia sp. Ramalina menziesii 5 Principal Species of Lichen Usnea sp. in California Coast Range Evernia prunastri How lichen interacts with mercury in air Dry deposition of Hg Fungus Hg0 Bi-directional exchange of Hg0 HgII Algae Wet deposition of MMHg MMHg Fun Facts about Lichen and Hg • Rapid uptake of Hg, especially HgII • Residence time of Hg of ~5 years (Nieboer and Richardson, 1981) • Time-integrated Hg accumulation rate reflects temporal variations in atmospheric Hg loads (Wittig, 1993) Previous Work: Methylmercury in Ramalina menziesii along a coastal fog gradient C&EN, 2016 Weiss-Penzias et al., 2019 Pros and Cons of Using Lichens as a Bioindicator of Atmospheric Deposition Pros Cons • Less expensive than using instrumentation. • The metal uptake and bioaccumulation depend on its bioavailability, the amount of • Better measure of the net supply of the deposition, pH, species, metabolic growth metal to terrestrial ecosystems than data rate and surface area, which are all affected from wet deposition measurements or by climatic and environmental stress model calculation. factors. • Hg contamination around point sources or • Potential for poor distribution of lichen “hot spots” can be easily depicted.
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