Production of Scorpion Antivenom from Little
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Honeybee (Apis Mellifera) and Bumblebee (Bombus Terrestris) Venom: Analysis and Immunological Importance of the Proteome
Department of Physiology (WE15) Laboratory of Zoophysiology Honeybee (Apis mellifera) and bumblebee (Bombus terrestris) venom: analysis and immunological importance of the proteome Het gif van de honingbij (Apis mellifera) en de aardhommel (Bombus terrestris): analyse en immunologisch belang van het proteoom Matthias Van Vaerenbergh Ghent University, 2013 Thesis submitted to obtain the academic degree of Doctor in Science: Biochemistry and Biotechnology Proefschrift voorgelegd tot het behalen van de graad van Doctor in de Wetenschappen, Biochemie en Biotechnologie Supervisors: Promotor: Prof. Dr. Dirk C. de Graaf Laboratory of Zoophysiology Department of Physiology Faculty of Sciences Ghent University Co-promotor: Prof. Dr. Bart Devreese Laboratory for Protein Biochemistry and Biomolecular Engineering Department of Biochemistry and Microbiology Faculty of Sciences Ghent University Reading Committee: Prof. Dr. Geert Baggerman (University of Antwerp) Dr. Simon Blank (University of Hamburg) Prof. Dr. Bart Braeckman (Ghent University) Prof. Dr. Didier Ebo (University of Antwerp) Examination Committee: Prof. Dr. Johan Grooten (Ghent University, chairman) Prof. Dr. Dirk C. de Graaf (Ghent University, promotor) Prof. Dr. Bart Devreese (Ghent University, co-promotor) Prof. Dr. Geert Baggerman (University of Antwerp) Dr. Simon Blank (University of Hamburg) Prof. Dr. Bart Braeckman (Ghent University) Prof. Dr. Didier Ebo (University of Antwerp) Dr. Maarten Aerts (Ghent University) Prof. Dr. Guy Smagghe (Ghent University) Dean: Prof. Dr. Herwig Dejonghe Rector: Prof. Dr. Anne De Paepe The author and the promotor give the permission to use this thesis for consultation and to copy parts of it for personal use. Every other use is subject to the copyright laws, more specifically the source must be extensively specified when using results from this thesis. -
Effects of Brazilian Scorpion Venoms on the Central Nervous System
Nencioni et al. Journal of Venomous Animals and Toxins including Tropical Diseases (2018) 24:3 DOI 10.1186/s40409-018-0139-x REVIEW Open Access Effects of Brazilian scorpion venoms on the central nervous system Ana Leonor Abrahão Nencioni1* , Emidio Beraldo Neto1,2, Lucas Alves de Freitas1,2 and Valquiria Abrão Coronado Dorce1 Abstract In Brazil, the scorpion species responsible for most severe incidents belong to the Tityus genus and, among this group, T. serrulatus, T. bahiensis, T. stigmurus and T. obscurus are the most dangerous ones. Other species such as T. metuendus, T. silvestres, T. brazilae, T. confluens, T. costatus, T. fasciolatus and T. neglectus are also found in the country, but the incidence and severity of accidents caused by them are lower. The main effects caused by scorpion venoms – such as myocardial damage, cardiac arrhythmias, pulmonary edema and shock – are mainly due to the release of mediators from the autonomic nervous system. On the other hand, some evidence show the participation of the central nervous system and inflammatory response in the process. The participation of the central nervous system in envenoming has always been questioned. Some authors claim that the central effects would be a consequence of peripheral stimulation and would be the result, not the cause, of the envenoming process. Because, they say, at least in adult individuals, the venom would be unable to cross the blood-brain barrier. In contrast, there is some evidence showing the direct participation of the central nervous system in the envenoming process. This review summarizes the major findings on the effects of Brazilian scorpion venoms on the central nervous system, both clinically and experimentally. -
The First Data on the Fauna and Geographical Distribution of Medically Important Scorpions in Golestan Province, Northeast of Iran
The First Data on the Fauna and Geographical Distribution of Medically Important Scorpions in Golestan Province, Northeast of Iran Aioub Sozadeh Golestan University of Medical Sciences and Health Services Ehsan Allah Kalteh Golestan University of Medical Sciences and Health Services Shahin Saeedi Golestan University of Medical Sciences and Health Services Mulood Mohammmadi Bavani ( [email protected] ) Department of Medical Entomology and Vector Control, School of Public Health, Urmia University of Medical Sciences Research note Keywords: Scorpion, fauna, spatial distribution, Iran, Golestan Posted Date: December 3rd, 2020 DOI: https://doi.org/10.21203/rs.3.rs-117232/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/14 Abstract Objectives: this study was conducted to determine the medically relevant scorpion’s species and produce their geographical distribution in Golestan Province for the rst time, to collect basic information to produce regional antivenom. Because for scorpion treatment a polyvalent antivenom is use in Iran, and some time it failed to treatment, for solve this problem govement decide to produce regional antivenom. Scorpions were captured at day and night time using ruck rolling and Ultra Violet methods during 2019. Then specimens transferred to a 75% alcohol-containing plastic bottle. Finally the specimens under a stereomicroscope using a valid identication key were identied. Distribution maps were introduced using GIS 10.4. Results: A total of 111 scorpion samples were captured from the province, all belonging to the Buthidae family, including Mesobuthus eupeus (97.3%), Orthochirus farzanpayi (0.9%) and Mesobuthus caucasicus (1.8%) species. -
Sequence Analysis of Lysozyme C from the Scorpion Mesobuthus Eupeus Venom Glands Using Semi- Nested RT-PCR
Iranian Red Crescent Medical Journal ORIGINAL ARTICLE Sequence Analysis of Lysozyme C from the Scorpion Mesobuthus Eupeus Venom Glands Using Semi- Nested RT-PCR M Baradaran1*, A Jolodar2, A Jalali3, Sh Navidpour4, F Kafilzadeh5 1Toxicology Research Center, Jundishapur University of Medical Sciences, Ahvaz, Iran; 2Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran; 3Department of Pharmacology and Toxi- cology, School of Pharmacy, Toxicology Research Center, Jundishapur University of Medical Sciences, Ahvaz, Iran; 4Veterinary Parasitology Department of Razi Vaccine and Serum Research Institute, Karaj, Iran; 5Azad Islamic University, Jahrom Branch, Jahrom , Iran Abstract Background: Lysozyme is an antimicrobial protein widely distributed among eukaryotes and prokaryotes and take part in protecting microbial infection. Here, we amplified cDNA of MesoLys-C, a c-type lysozyme from the most common scorpion in Khuzestan Province, Southern Iran. Methods: Scorpions of Mesobuthus eupeus were collected from the Khuzestan Province. Using RNXTM solu- tion, the total RNA was extracted from the twenty separated venom glands. cDNA was synthesized with extract- ed total RNA as template and modified oligo(dT) as primer. In order to amplify cDNA encoding a lysozyme C, semi-nested RT-PCR was done with the specific primers. Follow amplification, the fragment was sequenced. Results: Sequence determination of amplified fragment revealed that MesoLys-C cDNA had 438 bp, encoding for 144 aa residues peptide with molecular weight of 16.702 kDa and theoretical pI of 7.54. A putative 22-amino- acids signal peptide was identified. MesoLys-C protein was composed of one domain belonged to c-type lyso- syme/alphalactalbumin. -
10/19/2020 Release for Publication: 04/30/2021
Measuring the metafrontier efficiencies and technology gaps of dried apricot farms in different 63 agro-ecological zones Gunduz, O.; Aslan, A.; Ceyhan, V.; Bayramoglu, Z. Measuring the metafrontier efficiencies and technology gaps of dried apricot farms in different agro-ecological zones Reception of originals: 10/19/2020 Release for publication: 04/30/2021 Orhan Gunduz PhD in Agricultural Economics Institution: Malatya Turgut Ozal University, Faculty of Agriculture, Department of Agricultural Economics Address: Battalgazi campus, P.O. Box: 44210, Battalgazi, Malatya, Turkey E-mail: [email protected] Ahmet Aslan MSc in Agricultural Economics Institution: Malatya Apricot Research Institute Address: Asagibaglar mah. No:163, P.O. Box: 44090,Yesilyurt, Malatya, Turkey E-mail: [email protected] Vedat Ceyhan PhD in Agricultural Economics Institution: Ondokuz Mayis University, Faculty of Agriculture, Department of Agricultural Economics Address: Kurupelit Campus, P.O. Box:55139, Atakum, Samsun, Turkey E-mail: [email protected] Zeki Bayramoglu PhD in Agricultural Economics Institution: Selcuk University, Faculty of Agriculture, Department of Agricultural Economics Address: Alaeddin Keykubat Campus, Akademi Mah. P.O. Box: 42130, Selcuklu, Konya, Turkey E-mail: [email protected] Abstract Dried apricot (Prunus armeniaca L.), which is extensively produced in Malatya region, is one of the most exported crop in the Turkish agriculture. Malatya is not only in Turkey, but also is the most producer region of whole world. Apricot is cultivated in all zones of Malatya under varying agro-ecological conditions that reflect production technology. Due to the conditions, performance of the farms and output quantity also varies. Thus, the present research focused to the production efficiency (TE) and technological gaps (TGR) of the farms in the different agro-ecological zones of Malatya, which is six, using stochastic metafrontier approach. -
Determination of Potential of Malatya Province in Terms of Tourism Marketing
KMÜ Sosyal ve Ekonomi̇ k Araştirmalar Dergi̇ si 15 (24): 01-11, 2013 ISSN: 1309-9132, www.kmu.edu.tr Turizm Pazarlaması Açısından Malatya İlinin Potansiyelinin Belirlenmesi Ayda GÖK 1 Hakan TUNA 2 1 İnönü Üniversitesi, Kale Meslek Yüksekokulu, Dış Ticaret ve Pazarlama Bölümü, MALATYA 2 İnönü Üniversitesi, Kale Meslek Yüksekokulu, Turizm ve Otel İşletmeciliği Bölümü, MALATYA Özet 20. yüzyılın sonlarına doğru hızla gelişen turizm hareketleri daha çok deniz, güneş ve kum ilişkisine dayanmaktaydı. Fakat günümüzde deniz, güneş, kum üçlüsünden doğa ve kültür turizmine yönelik bir değişim görülmektedir. Böylece, turizm faaliyetleri sadece sahil kenarlarında değil, doğal ve kültürel açıdan cazip iç kesimlerde de önemli gelir kaynaklarından birisi haline gelmektedir. Malatya ili doğa ve kültür turizmi açısından zenginlikleri bulunan, çok eski bir tarihi geçmişi olan bir ilimizdir. Eskimalatya ve Aslantepe’de bulunan eserler tarihi zenginlikler olarak dikkat çekerken; Sultansuyu, Karakaya Barajı ve Levent Vadisi doğa turizmi kapsamında Malatya'nın keşfedilmeyi bekleyen değerleridir. Malatya’da halk oyunları, geleneksel giyim, geleneksel el sanatları, mutfak kültürü önemli değerleri oluşturmaktadır. Ayrıca Malatya'nın UNESCO Dünya Kültür Mirası Listesinde yer alan Nemrut Dağı'na bağlantısı turistik çekiciliğini arttırmaktadır. Bu çalışmanın amacı, Malatya ilinin turizm potansiyelini ortaya koymak, yapılabilecek alternatif turizm türlerini saptamaktır. Malatya ilinde turizmin gelişmesinde etkili olan yerel yöneticiler ve uzmanlarla anket yapılarak -
Comparison of Antivenom Effects Between Pediatric and Adult Patients Presented to Emergency Department with Scorpion Stings
Available online at www.medicinescience.org Medicine Science International ORIGINAL ARTICLE Medical Journal Medicine Science 2020;9(1):109-13 Comparison of antivenom effects between pediatric and adult patients presented to emergency department with scorpion stings Ertugrul Altinbilek1, Kaan Yusufoglu1, Abdullah Algin2, Sahin Colak3 1 University of Health Sciences Sisli Hamidiye Etfal Training and Research Hospital, Department of Emergency Medicine, Istanbul, Turkey 2University of Health Sciences Umraniye Training and Research Hospital, Department of Emergency Medicine, Istanbul, Turkey 3University of Health Sciences Haydarpasa Numune Training and Research Hospital, Department of Emergency Medicine, Istanbul, Turkey Received 15 August 2019; Accepted 23 September 2019 Available online 24.02.2020 with doi: 10.5455/medscience.2019.08.9148 Abstract The aim of this study was to compare the use of antivenom, and admission to ICU, scorpinism between adult and pediatric patients. This study included 99 patients who were admitted to the emergency department with scorpion sting within 1 year. Patients’ demographics including age and gender, and clinical findings such as ionized Ca values, body region of sting contact and complications were recorded from the patient files and hospital records. In addition, regarding management of patients with scorpionism the use of antivenoms, admission to intensive care unit and complications developed by the patients were also recorded. Patients were divided into two groups according to age as the pediatric group including patients aged ≤ 18 years (Group 1) old and the adult group consisting of patients aged> 18 years old (Group 2). Antivenom administration was performed in 12 patients (12.2%). Antivenom was administered in 38% (n=8) of the patients in Group 1 and 5.13% (n=4) of the patients in Group 2. -
A Global Accounting of Medically Significant Scorpions
Toxicon 151 (2018) 137–155 Contents lists available at ScienceDirect Toxicon journal homepage: www.elsevier.com/locate/toxicon A global accounting of medically significant scorpions: Epidemiology, major toxins, and comparative resources in harmless counterparts T ∗ Micaiah J. Ward , Schyler A. Ellsworth1, Gunnar S. Nystrom1 Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA ARTICLE INFO ABSTRACT Keywords: Scorpions are an ancient and diverse venomous lineage, with over 2200 currently recognized species. Only a Scorpion small fraction of scorpion species are considered harmful to humans, but the often life-threatening symptoms Venom caused by a single sting are significant enough to recognize scorpionism as a global health problem. The con- Scorpionism tinued discovery and classification of new species has led to a steady increase in the number of both harmful and Scorpion envenomation harmless scorpion species. The purpose of this review is to update the global record of medically significant Scorpion distribution scorpion species, assigning each to a recognized sting class based on reported symptoms, and provide the major toxin classes identified in their venoms. We also aim to shed light on the harmless species that, although not a threat to human health, should still be considered medically relevant for their potential in therapeutic devel- opment. Included in our review is discussion of the many contributing factors that may cause error in epide- miological estimations and in the determination of medically significant scorpion species, and we provide suggestions for future scorpion research that will aid in overcoming these errors. 1. Introduction toxins (Possani et al., 1999; de la Vega and Possani, 2004; de la Vega et al., 2010; Quintero-Hernández et al., 2013). -
Full Text Oral Presentations
4TH INTERNATIONAL REGIONAL DEVELOPMENT CONFERENCE PROCEEDING BOOK 21-23 SEPTEMBER 2017 TUNCELİ / TURKEY ORGANIZERS MUNZUR UNIVERSITY FIRAT DEVELOPMENT AGENCY Editor Asst. Prof. Dr. Ekrem AKBULUT i Prepare for Printing Res. Asst. Faruk SERİN Asst. Prof. Dr. Muhammet GÜL First Edition - 2017 © International Regional Development Conference Publisher Fırat Development Agency Malatya / Turkey www.fka.gov.tr ISBN: 978-605-67754-0-6 ii © International Regional Development Conference CONTENTS CONTENTS .................................................................................................................................. iii WELCOME TO IRDC’17! ........................................................................................................... x CONFERENCE CHAIRS............................................................................................................ xi CONFERENCE INFO ................................................................................................................ xv KEYNOTE SPEAKERS ............................................................................................................ xvi FULL TEXT ORAL PRESENTATIONS ........................................................................................... 1 A Research on Examining Regional Development Agencies’ Corporate Identity Components ............................................................................................................................................. 2 A Study on Economic Crisis and Its Effects on Tourism -
A New Species of Ananteris (Scorpiones: Buthidae) from Panama
A new species of Ananteris (Scorpiones: Buthidae) from Panama Roberto J. Miranda & Luis F. de Armas February 2020 — No. 297 Euscorpius Occasional Publications in Scorpiology EDITOR: Victor Fet, Marshall University, ‘[email protected]’ ASSOCIATE EDITOR: Michael E. Soleglad, ‘[email protected]’ Euscorpius is the first research publication completely devoted to scorpions (Arachnida: Scorpiones). Euscorpius takes advantage of the rapidly evolving medium of quick online publication, at the same time maintaining high research standards for the burgeoning field of scorpion science (scorpiology).Euscorpius is an expedient and viable medium for the publication of serious papers in scorpiology, including (but not limited to): systematics, evolution, ecology, biogeography, and general biology of scorpions. Review papers, descriptions of new taxa, faunistic surveys, lists of museum collections, and book reviews are welcome. Derivatio Nominis The name Euscorpius Thorell, 1876 refers to the most common genus of scorpions in the Mediterranean region and southern Europe (family Euscorpiidae). Euscorpius is located at: https://mds.marshall.edu/euscorpius/ Archive of issues 1-270 see also at: http://www.science.marshall.edu/fet/Euscorpius (Marshall University, Huntington, West Virginia 25755-2510, USA) ICZN COMPLIANCE OF ELECTRONIC PUBLICATIONS: Electronic (“e-only”) publications are fully compliant with ICZN (International Code of Zoological Nomenclature) (i.e. for the purposes of new names and new nomenclatural acts) when properly archived and registered. All Euscorpius issues starting from No. 156 (2013) are archived in two electronic archives: • Biotaxa, http://biotaxa.org/Euscorpius (ICZN-approved and ZooBank-enabled) • Marshall Digital Scholar, http://mds.marshall.edu/euscorpius/. (This website also archives all Euscorpius issues previously published on CD-ROMs.) Between 2000 and 2013, ICZN did not accept online texts as “published work” (Article 9.8). -
The Moss Flora of Arapgir (Malatya/Turkey) District Mevlüt ALATAŞ *1, Nevzat BATAN 2 1 Tunceli Üniversitesi, Mühendislik Fa
Research article/Araştırma makalesi The moss flora of Arapgir (Malatya/Turkey) district Mevlüt ALATAŞ *1, Nevzat BATAN 2 1 Tunceli Üniversitesi, Mühendislik Fakültesi, Biyomühendislik Bölümü, Tunceli, Turkey 2Karadeniz Teknik Üniversitesi, Maçka Meslek Yüksekokulu, Trabzon, Turkey Abstract In this study, the moss flora of the Arapgir District (Malatya) was investigated between April and October 2015. In total 45 taxa, belonging to 11 families and 25 genera were determined by identifying moss specimens collecting the study area. At the same time all the taxa are new records from Malatya province according to the grid- square system of Henderson (1961) six taxa are new records for B9 grid square. While the largest families in terms of number of taxa are Pottiaceae (14), Brachytheciaceae (11), Orthotrichaceae and Bryaceae (4), the largest genera are Syntrichia (5), Orthotrichum and Tortula (4). Also, the life forms of the taxa which were examined in terms of ecological and floristic have been analyzed. From life forms; while Turf life form ranks the first, Mr life form ranks the second. Finally, the most taxa within the floristic list in terms of some ecological characteristics are xerophyt, photophyt and subneutrophyt. Key words: moss, flora, Arapgir, Malatya, Turkey ---------- ---------- Arapgir ilçesi (Malatya) karayosunu florası Özet Bu çalışmada, 2015 yılının Nisan ve Ekim ayları arasında Arapgir (Malatya) ilçesinin karayosunu florası araştırılmıştır. Araştırma alanından toplanan karayosunu örneklerinin teşhis edilmesi sonucunda, 11 familya ve 25 cinse ait 45 takson tespit edilmiştir. Tamamı Malatya ili için yeni kayıt olan taksonların aynı zamanda 6 tanesi Henderson (1961) kareleme sistemine göre B9 karesi için yenidir. Takson sayısı bakımından en kalabalık familyalar Pottiaceae (14), Brachytheciaceae (11), Orthotrichaceae ve Bryaceae (4) iken en kalabalık cinsler Syntrichia (5), Orthotrichum ve Tortula (4)’dır. -
Malatya Yeşilyurt İlçesinin Kırsal Turizm Potansiyelinin Değerlendirilmesi Için Çözüm Önerileri
Türk Bilimsel Derlemeler Dergisi 7 (2): 34-37, 2014 ISSN: 1308-0040, E-ISSN: 2146-0132, www.nobel.gen.tr Malatya Yeşilyurt İlçesinin Kırsal Turizm Potansiyelinin Değerlendirilmesi için Çözüm Önerileri Fürüzan ASLAN1* Bülent YILMAZ1 Atilla ATİK1 Duygu DOĞAN1 Oğuz ATEŞ1 1İnönü Üniversitesi, Güzel Sanatlar ve Tasarım Fakültesi, Peyzaj Mimarlığı Bölümü, Malatya *Sorumlu Yazar: Geliş Tarihi: 03 Temmuz 2014 E-posta: [email protected] Kabul Tarihi: 22 Ağustos 2014 Özet Turizm, son yıllarda ülkelere büyük getiriler sağlayan bir sektör haline gelmiştir. Ülkeler, mevcut kaynakları kullanarak farklı turist istek ve ihtiyaçlarını karşılayabilmek adına yeni turistik ürünler oluşturmaya yönelmişlerdir. Farklı turistik ürünlerin yaratılmasıyla “alternatif turizm” kavramı gelişmiştir. Alternatif turizm türlerinden kırsal turizm, hem kırsal yerleşmelerle iç içe olan, hem de doğal kaynaklara dayalı bir turizm türüdür. Tarımsal faaliyetlerin olduğu kırsal alanlarda tarım faaliyetlerinin yanı sıra yerel istihdamın teşvik edilmesi, refah seviyesinin yükseltilerek kırsal kalkınmanın sağlanmasında bir araç olarak değerlendirilmektedir. Doğu Anadolu Bölgesinin Yukarı Fırat Bölümünde Malatya Ovası ile Beydağları arasındaki tepelik arazide Derme çayı vadisinde yer alan Yeşilyurt ilçesi, Malatya il merkezinin 9 km. güneybatısındadır. Kırsal turizm potansiyeli açısından değerlendirilmeye hazır oldukça zengin bir çeşitliliğine sahiptir. Ancak bu zenginlikler yıllarca göz ardı edilmiş ve bölge turizm sektöründen yeterli payı alamamıştır. Bu bildiride öncelikle