DOCSLIB.ORG

Deer Embryos by the Somatic Nuclear Transfer Technique

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Deer Embryos by the Somatic Nuclear Transfer Technique Int. J. Morphol., 24(2):285-292, 2006. Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique Obtención de Embriones de Pudú (Pudu pudu) Mediante Técnicas de Transferencia Nuclear Somática *Felipe Venegas; **Michel Guillomot; **Xavier Vignon; **Jean-Luc Servely; **Chistophe Audouard; *Enrique Montiel; **Daniel Le Bourhis; **Sandrine Perón; ***Pilar Soto & *Mariana Rojas VENEGAS, F.; GUILLOMOT, M.; VIGNON, X.; SERVELY, J-L-; AUDOUARD, C.; MONTIEL, E.; LE BOURHIS, D.; PERÓN, S.; SOTO, P. & ROJAS, M. Obtaiment of Pudu pudu deer embryos by the somatic nuclear transfer technique. Int. J. Morphol., 24(2):285-292, 2006. SUMMARY: The objective of this project is to produce pudu embryos, obtained by means of fibroblast transfer taken from the ear of one animal, to the oocytes of a domestic ruminant such as cattle. In further work, the pudu embryos will be transferred to the uterus of synchronized recipient females of another species. Biopsies 1mm in size were obtained from the outer rim of the ear of two male pudu deer belonging to the Buin Zoological park, Santiago, Chile. The cell lines have been established and kept according to protocols used in cattle. The oocytes are obtained through a puncture in the oocyte-cumulus complex (OCC) from cow ovaries recovered from the butcher. Each oocyte is enucleated and fused with an isolated fibroblast which is inserted beneath the pellucid zone. The fusion of cell membranes is achieved by means of electric shocks. In respect to the timetable, we have observed that a stage of two blastomers is reached on the second day, and morulae of 8 to 16 cells on the third day. On the fourth day it has differenciated as a blastocyst and on the seventh day it finally hacht from the pellucid zone. The obtainment of embryonic blastocytes indicates that it is possible to obtain pudu embryos through heterospecific cloning, even though the percentage of success is relatively low. The viability of the embryos obtained in this manner after in uterus transfer remains still to be verified. KEY WORDS: Pudu deer; Cervidae; Ruminant; Cloning. INTRODUCTION The pudu (Pudu pudu) is the smallest of South rate caused by genetic illnesses (Codeff, 1991 and Mella et American deer species; it reaches no more than 40 cm at the al., 2002). withers and weights less than 15 kg (Blumberg, 2002). It is a deer native to Chile and Argentine and can be found from The Pudu is a ruminant belonging to the Order sea level up to 1700 meters of altitude. In Chile it is distributed Artiodactyle, Family Odocoileinae, which incluyes the from 35º10'S (VII Region) to 46º45'S (XI Region). This genders Hippocamelus and Pudu. In turn, two species belong species has been considered endangered in accordance with to the gender Pudu: Pudu mephistophiles, which inhabits Perú the Red Book from the World Union for Nature (U.I.C.N.) and Colombia, and the southern pudu or Pudu pudu native of and the appendix 1 of the CITES (Glade, 1993). Chile and Argentine. The pudu has 70 chromosomes (Spotorno & Fernández-Donoso, 1975 and Spotorno & Walker, 2000). This species has suffered from the gradual destruction of its habitat, poaching and chasing by feral dogs. At present Activity patterns of the pudu in its natural habitat have the population can be found forming small isolated groups been studied (Wetterberg, 1972; Eldridge et al., 1987), as well that cannot reproduce between them. As a consequence, the as its behavior in captivity (Macnamara & Eldridge, 1987 increased in-breeding has resulted in an increase in the death and Cortes et al., 1988). Studies have also been carried out in * Laboratory of Compared Embryology; ICBM; Department of Anatomy and Developmental Biology; Medical School, University of Chile. ** Institut National de la Recherche Agronomique; Jouy en Josas; Francia. *** Buin-Zoological Park, Santiago - Chile. Grant ECOS-CONICYT C03 B02 285 VENEGAS, F.; GUILLOMOT, M.; VIGNON, X.; SERVELY, J-L-; AUDOUARD, C.; MONTIEL, E.; LE BOURHIS, D.; PERÓN, S.; SOTO, P. & ROJAS, M. order to establish the relationship between LH and testosterone species at risk of extinction. These techniques include Artifi- levels to the development of the antlers in the male deer (Re- cial Insemination, Embryonic Transplant, In Vitro Fertilization yes et al., 1993). and the intracytoplasmic injection of sperm cells (Comizzoli et al., 2000). At present, assisted reproduction technique is The seasonal changes of the pudu testicles are also being fine-tunned in deer species. The “Proyecto Arca” in well known, as well as the variations of cortisol, IGF-1 and Argentina is an example. Their objective is to return the “father triodothyronine levels in males (Reyes et al., 1997). Other David’s deer” (Elaphurus davidianus) to it’s country of origin, endocrine studies in adult males pudu, concerning the levels not only because this species has become extinct in China, of FSH, LH, testosterone and prolactine in adult males have but because of the necessity of the development of a been published (Bubenik et al., 2000). Bartos et al. (1998) methodological work applicable to native deer. studied the hormonal levels of two males housed together with females, in order to assess dominance characteristics. By other Some zoos have successfully managed to keep pudus hand, Hershkovitz (1982), reported that pudu have a in captivity, as opposed to other south american endangered gestational period of 7 months (between 202 to 223 days). cervids such as the Taruca (Hippocamelus antisense) and the southern Huemul (Hippocamelus bisulcus), both of which The publication by Schurer & Sliwa (2002) provides species do not survive in captivity (Rojas et al., 2004, 2005). much more information: the average length of gestation Hick (1969) reported the unsuccessful raising of a pudu at observed in this study was 210 days; the average weight at Cologne Zoo. Pudus are currently kept at Doué-la-Fontaine birth was 890 g (55 newborn animals were assessed), the zoo in France and in the Wild Animal Park of the Zoological female usually being as heavy as the males. Offspring below Society of San Diego, USA, where reproduction of the 600 g did not survive, nor did those weighing more than 1000 shouther huemul has been achieved. (Hershkovitz). g Guzmán et al. (1986), described some characteristics of birth: the female gives birth to a single foal of reddish tabby In South American zoos, banks with of pudu sperm color, with whitish stripes along the back and thighs. These are kept in order to aid artificial insemination of this species stripes disappear at the age of three months. (Santiago Metropolitan Zoo, in Chile and also in Buenos Ai- res Zoo, Argentina). Diverse activities have been carried out The pudu placenta, like that of other deers is in parallel to generate an increase in the reproduction rate in oligocotyledonary, villous, and epithelio-chorial. The placenta other vulnerable and endangered deer all over the world. had only one intact cotyledon and a second atrophying Nevertheless, classic techniques of embryo production have cotyledon. The larger cotyledon observed measured 8 x 1 cm. proved to be difficult. From this point of view, Locatelly & The placenta weighed 110 g. There is intensive interdigitation Mermillod (2005) stated that reproductive biotechnology and of villi with the caruncular epithelial folds. Thus, an intimate assisted reproduction methods in endangered cervids could contact between trophoblast and endometrial epithelium is help conservation programs. established. The caruncles are composed of stroma and blood vessels, covered by endometrial epithelium. There are no Restoration of species by transfer of somatic nuclei into glands (Harrison & Hamilton, 1952; Hamilton et al., 1960; enucleated recipient oocytes has already been considered Sadlier, 1987 and Drion et al., 2003) (Moratelli et al., 2002). Calves of an endangered breed of cattle (Enderby Island) adapted to extreme climatic conditions were Although natural reproduction should be the preferred born after nuclear transfer of granulose cells into enucleated method in order to increase the population of a certain species, oocytes from domestic cows and the ulterior transfer of the when wild populations are decreasing in number this is not resulting embryos into domestic cow recipients (Wells et al., longer possible. The pudu reproduction program developed 1998, 2000). Additionally, a pregnancy was observed after the by Reyes (1991) has been a contribution to the country but transfer of embryos reconstructed from cells of argali (Ovis has not been able to improve the situation. Reproduction ammon) and enucleated oocytes of domestic sheep (White et technology could be an important support by establishing al., 1999). In the giant panda, blastocysts have also been obtained genetic reserve banks of endangered animals in risk of after nuclear transfer of panda cells into rabbit enucleated extinction (Holt et al., 1996; Holt & Pickard, 1999; Watson oocytes (Chen, 1999). Six day embryos of the Saola deer & Holt, 2001 and Holt et al., 2002) and for the conservation (Pseudoryx Nghetinhensis) have also been obtained by means of wild animals. of the cloning method and are currently kept frozen, waiting to be implanted on to a surrogate mother (Service Presse INRA). It is thought that reproduction of wild species could be aided by the use of stored genetic material (Wildt et al., Furthermore, it has been demonstrated that bovine 1997). from zoological parks and universities, specially those oocyte cytoplasm could also be used as recipient for somatic 286 Obtaiment of Pudu pudu deer embryos by the somatic nuclear transfer technique. Int. J. Morphol., 24(2):285-292, 2006. cells from different mammalian species (Dominko et al., Once in the laboratory, the sample was shaved and rinsed to 1999). Cloning might even serve a useful purpose with species remove the loosen hairs. The sample was then placed on a that have never bred in captivity, such as the saola (Comizzoli culture dish were micro fragments of tissue were cut and et al., 2000) y el huemul (Rojas et al., 2005).
Load more

Recommended publications
  • O18934 Plard, F., Bonenfant, C. and Gaillard, JM 2011
    Oikos O18934 Plard, F., Bonenfant, C. and Gaillard, J. M. 2011. Re- visiting the allometry of antlers among deer species: male-male sexual competition as a driver. – Oikos 120: 601–606. Table A1. Data basis. Sexual size dimorphism (SSD) was measured by the residuals of the regression of male body mass against female body mass. Breeding group sizes (BGS) were divided into three categories: group of one or two animals (A), group of three, four and five animals (B), and groups larger than five animals (C). The different mating tactics (MT) were harem (H), territorial (T) and tending (F). Non-available mating tactics (NA) and monogamous species (M) are indicated. Sub-family Genus Species Common name Male Female SSD BGS MT Ref Antler Body Body length mass mass Cervinae Axis axis chital 845 89.5 39 0.58 C F 1,2,3 Cervinae Axis porcinus hog deer 399 41 31 0.07 C F 2,3,4,5 Cervinae Cervus albirostris white-lipped deer 1150 204 125 0.06 C H 1,6 Cervinae Cervus canadensis wapiti 1337 350 250 –0.21 C H 3,8,9 Cervinae Cervus duvaucelii barasingha 813 236 145 0.03 C H 1,3,10,11 Cervinae Cervus elaphus red deer 936 250 125 0.34 C H 1,2,3 Cervinae Cervus eldi Eld’s deer 971.5 105 67 0.12 C H 1,2,3 Cervinae Cervus nippon sika deer 480 52 37 0.1 B T 1,2,3,9,12,13 Cervinae Cervus timorensis Timor deer 675 95.5 53 0.29 C H 1,9 Cervinae Cervus unicolor sambar 1049 192 146 –0.18 B T 1,2,3,7 Cervinae Dama dama fallow deer 615 67 44 0.15 C T 1,2,3,14 Cervinae Elaphurus davidianus Père David’s deer 737 214 159 –0.17 C H 1,2,3 Muntiacinae Elaphodus cephalophus
    [Show full text]
  • Pudu in a Chilean National Park
    547 Pudu in a Chilean National Park Gary 8. Wetterberg The Chilean pudu Pudu pudu, the smallest American deer, is on the world list of endangered species in the IUCN Red Data Book. One of its few remaining refuges is in the Vicente Perez Rosales National Park. This is in the Lake District of southern Chile, the 'Switzerland of South America', between the Puyehue National Park to the north, and the Nahuel Huapi National Park in Argentina on the east. There are very few records on the fauna of this park, which covers 243,000 hectares, and is part of the Patagonian Subdivision of the Neotropical Faunal Region. Like an Island In many ways, Chile is like an island, cut off by the Atacama Desert on the north, the Andes to the east, the Patagonian ice fields and fiords to the south, and the Pacific on the west. This geo- graphical isolation has permitted the development of a unique biota, and Chilean wildlife exhibits some of the characteristics of island fauna such as narrow endemics and few competitors. The pudu is descended from the deer that migrated from North America in the late Tertiary period (Simpson 1950). The species is primarily of Chilean origin and distribution, although it is frequently encountered in adjacent areas of Argentina, and is present in Bolivia (Walker, 1964). It was discovered and named in 1782 by the Jesuit Juan Ignacio Molina, the 'father of Chilean natural history' (Osgood, 1943). Other species of the genus are found in Ecuador and Peru (Grimwood, 1968), and Brazil (Hershkovitz, 1958).
    [Show full text]
  • Sexual Selection and Extinction in Deer Saloume Bazyan
    Sexual selection and extinction in deer Saloume Bazyan Degree project in biology, Master of science (2 years), 2013 Examensarbete i biologi 30 hp till masterexamen, 2013 Biology Education Centre and Ecology and Genetics, Uppsala University Supervisor: Jacob Höglund External opponent: Masahito Tsuboi Content Abstract..............................................................................................................................................II Introduction..........................................................................................................................................1 Sexual selection........................................................................................................................1 − Male-male competition...................................................................................................2 − Female choice.................................................................................................................2 − Sexual conflict.................................................................................................................3 Secondary sexual trait and mating system. .............................................................................3 Intensity of sexual selection......................................................................................................5 Goal and scope.....................................................................................................................................6 Methods................................................................................................................................................8
    [Show full text]
  • Evaluating Mortality Sources for the Vulnerable Pudu Pudu Puda in Chile: Implications for the Conservation of a Threatened Deer
    Evaluating mortality sources for the Vulnerable pudu Pudu puda in Chile: implications for the conservation of a threatened deer E duardo A. Silva-RodrI´ guez,Claudio V erdugo,O.Alejandro A leuy J ames G. Sanderson,Gabriel R. Ortega-SolI´ s ,Felipe O sorio-ZU´ N˜ iga and D aniel G onzA´ lez-AcuN˜ a Abstract We assessed the importance of potential sources of substantive environmental education to modify dog man- mortality for the Vulnerable southern pudu Pudu puda in agement near protected areas. southern Chile using the clinical records of wildlife re- habilitation centres, necropsies of animals found in the field Keywords Chile, domestic dog, poaching, Pudu puda, and a review of the diet of potential predators. To assess Puma concolor, roadkills, South America, temperate forest whether the identified mortality sources operate in nomi- nally protected areas, we conducted a camera-trap survey in two areas to determine the presence of pudus and their Introduction potential predators. Predation by domestic dogs Canis lupus familiaris and car collisions were the commonest causes of he southern pudu Pudu puda is one of the smallest pudu admissions to rehabilitation centres (35 of 44) and of Tdeer in the world reaching just 40 cm in height deaths of animals encountered opportunistically in the field (Hershkovitz, 1982). It inhabits the South American tem- (seven of 14). Field data suggest that poaching could also be perate rainforest of Chile and Argentina, where it is en- an important threat to pudus. Pudus were detected in both demic (Wemmer, 1998). The geographical distribution of areas surveyed, accounting for 15.6% of mammal detections.
    [Show full text]
  • Canada, Decembre 2008 Library and Bibliotheque Et 1*1 Archives Canada Archives Canada Published Heritage Direction Du Branch Patrimoine De I'edition
    ORGANISATION SOCIALE, DYNAMIQUE DE POPULATION, ET CONSERVATION DU CERF HUEMUL (HIPPOCAMELUS BISULCUS) DANS LA PATAGONIE DU CHILI par Paulo Corti these presente au Departement de biologie en vue de l'obtention du grade de docteur es sciences (Ph.D.) FACULTE DES SCIENCES UNIVERSITE DE SHERBROOKE Sherbrooke, Quebec, Canada, decembre 2008 Library and Bibliotheque et 1*1 Archives Canada Archives Canada Published Heritage Direction du Branch Patrimoine de I'edition 395 Wellington Street 395, rue Wellington Ottawa ON K1A0N4 Ottawa ON K1A0N4 Canada Canada Your file Votre reference ISBN: 978-0-494-48538-5 Our file Notre reference ISBN: 978-0-494-48538-5 NOTICE: AVIS: The author has granted a non­ L'auteur a accorde une licence non exclusive exclusive license allowing Library permettant a la Bibliotheque et Archives and Archives Canada to reproduce, Canada de reproduire, publier, archiver, publish, archive, preserve, conserve, sauvegarder, conserver, transmettre au public communicate to the public by par telecommunication ou par Plntemet, prefer, telecommunication or on the Internet, distribuer et vendre des theses partout dans loan, distribute and sell theses le monde, a des fins commerciales ou autres, worldwide, for commercial or non­ sur support microforme, papier, electronique commercial purposes, in microform, et/ou autres formats. paper, electronic and/or any other formats. The author retains copyright L'auteur conserve la propriete du droit d'auteur ownership and moral rights in et des droits moraux qui protege cette these. this thesis. Neither the thesis Ni la these ni des extraits substantiels de nor substantial extracts from it celle-ci ne doivent etre imprimes ou autrement may be printed or otherwise reproduits sans son autorisation.
    [Show full text]
  • List of 28 Orders, 129 Families, 598 Genera and 1121 Species in Mammal Images Library 31 December 2013
    What the American Society of Mammalogists has in the images library LIST OF 28 ORDERS, 129 FAMILIES, 598 GENERA AND 1121 SPECIES IN MAMMAL IMAGES LIBRARY 31 DECEMBER 2013 AFROSORICIDA (5 genera, 5 species) – golden moles and tenrecs CHRYSOCHLORIDAE - golden moles Chrysospalax villosus - Rough-haired Golden Mole TENRECIDAE - tenrecs 1. Echinops telfairi - Lesser Hedgehog Tenrec 2. Hemicentetes semispinosus – Lowland Streaked Tenrec 3. Microgale dobsoni - Dobson’s Shrew Tenrec 4. Tenrec ecaudatus – Tailless Tenrec ARTIODACTYLA (83 genera, 142 species) – paraxonic (mostly even-toed) ungulates ANTILOCAPRIDAE - pronghorns Antilocapra americana - Pronghorn BOVIDAE (46 genera) - cattle, sheep, goats, and antelopes 1. Addax nasomaculatus - Addax 2. Aepyceros melampus - Impala 3. Alcelaphus buselaphus - Hartebeest 4. Alcelaphus caama – Red Hartebeest 5. Ammotragus lervia - Barbary Sheep 6. Antidorcas marsupialis - Springbok 7. Antilope cervicapra – Blackbuck 8. Beatragus hunter – Hunter’s Hartebeest 9. Bison bison - American Bison 10. Bison bonasus - European Bison 11. Bos frontalis - Gaur 12. Bos javanicus - Banteng 13. Bos taurus -Auroch 14. Boselaphus tragocamelus - Nilgai 15. Bubalus bubalis - Water Buffalo 16. Bubalus depressicornis - Anoa 17. Bubalus quarlesi - Mountain Anoa 18. Budorcas taxicolor - Takin 19. Capra caucasica - Tur 20. Capra falconeri - Markhor 21. Capra hircus - Goat 22. Capra nubiana – Nubian Ibex 23. Capra pyrenaica – Spanish Ibex 24. Capricornis crispus – Japanese Serow 25. Cephalophus jentinki - Jentink's Duiker 26. Cephalophus natalensis – Red Duiker 1 What the American Society of Mammalogists has in the images library 27. Cephalophus niger – Black Duiker 28. Cephalophus rufilatus – Red-flanked Duiker 29. Cephalophus silvicultor - Yellow-backed Duiker 30. Cephalophus zebra - Zebra Duiker 31. Connochaetes gnou - Black Wildebeest 32. Connochaetes taurinus - Blue Wildebeest 33. Damaliscus korrigum – Topi 34.
    [Show full text]
  • Social Ecology of the Huemul at Torres Del Paine National Park, Chile
    Anales Instituto Patagonia (Chile), 2016. Vol. 44(1):25-38 25 Social Ecology of the huemul at Torres Del Paine National Park, Chile Ecología Social del huemul del Parque Nacional Torres Del Paine, Chile Gladys Garay1, Isaac M. Ortega2 & Oscar Guineo1 Abstract while yearlings were temporarily expelled from their In a ten-year study of the huemul (Hippocamelus natal group but remained within the home range, bisulcus) population at Torres del Paine National thus sometimes the three members of the group Park in southern Patagonia, we covered a wide were seen alone. These critical periods increased range of information regarding the ecology of this antagonism among huemuls, generating changes endanger deer. We determined the huemul social in their group structure. Movements associated structure, social behavior, reproductive season, with huemul in the park consisted of movements and associated movements throughout the year. within the home range, seasonal movements, Huemuls were observed in the park central area of dispersion of the yearlings, and movements during Sector Grey. When animals were located, natural the reproductive period. marks or scars, coloring of body and face, and antler characteristics were used to identify them. Key words: We also ear tagged sixteen fawns from 2002 to Hippocamelus bisulcus, social structure, 2008. Behavioral information was collected from reproduction, dispersion, Chilean Patagonia. all members with focal observations. We found established and transient social groups. Among Resumen the established we found family groups, solo Este es un estudio de la población de huemules female, and solo male, and among the transient (Hippocamelus bisulcus) del Parque Nacional Torres we found solo female, solo male, solo yearling, del Paine, en el sur de la Patagonia, durante 10 pair of yearlings, and mixed groups.
    [Show full text]
  • A Comprehensive Approach Towards the Systematics of Cervidae
    A peer-reviewed version of this preprint was published in PeerJ on 18 February 2020. View the peer-reviewed version (peerj.com/articles/8114), which is the preferred citable publication unless you specifically need to cite this preprint. Heckeberg NS. 2020. The systematics of the Cervidae: a total evidence approach. PeerJ 8:e8114 https://doi.org/10.7717/peerj.8114 A comprehensive approach towards the systematics of Cervidae Nicola S Heckeberg Corresp., 1, 2, 3 , Gert Wörheide 1, 2, 4 1 Department of Earth and Environmental Sciences, Palaeontology & Geobiology, Ludwig-Maximilians-Universität München, Munich, Germany 2 SNSB-Bayerische Staatssammlung für Paläontologie und Geologie, Munich, Germany 3 Leibniz Institute for Evolution and Biodiversity Science, Museum für Naturkunde, Berlin, Germany 4 Geobio-CenterLMU, Munich, Germany Corresponding Author: Nicola S Heckeberg Email address: [email protected] Systematic relationships of cervids have been controversial for decades. Despite new input from molecular systematics, consensus could only be partially reached. The initial, gross (sub)classification based on morphology and comparative anatomy was mostly supported by molecular data. The rich fossil record of cervids has never been extensively tested in phylogenetic frameworks concerning potential systematic relationships of fossil cervids to extant cervids. The aim of this work was to investigate the systematic relationships of extant and fossil cervids using molecular and morphological characters and make implications about their evolutionary history based on the phylogenetic reconstructions. To achieve these objectives, molecular data were compiled consisting of five nuclear markers and the complete mitochondrial genome of 50 extant and one fossil cervid species. Several analyses using different data partitions, taxon sampling, partitioning schemes, and optimality criteria were undertaken.
    [Show full text]
  • La Taruca, Huemul Del Norte
    La Taruca, Huemul del Norte 1 2 Copaquilla Título: La Taruca, Huemul del norte Autores: Jorge Herreros de Lartundo y Walter Sielfeld Kowald Fotografías: Jorge Herreros de Lartundo, Mauricio López Escobar, Víctor Quezada y Eduardo Katz Gaudlitz Revisores: Felipe Avendaño Pérez, Maritza Briones Barraza, José Manuel Valdivieso Quiroga y Mónica Piña Zepeda. Citar como: Herreros de Lartundo, J. y W. Sielfeld Kowald. 2011. La Taruca, Huemul del norte. Ministerio del Medio Ambiente. Arica, Chile. 64 pp. Inscripción I. S. B. N. N° Impreso en Gráfika Marmor Primera edición noviembre de 2011 500 ejemplares impresos. 3 Taruca, Maritzasecreto Briones Barraza de la naturaleza uién lo pudiera creer Me llama mucho la atención; tan magnífica belleza la rareza numérica de tu especie, que podemos encontrar, que te alimentas apenas amanece Qsecreto de la naturaleza. y cuando se esconde el sol, Si sólo en los cuentos de hadas al mando de la hembra con timidez, y en manada, en el bosque te asomabas, sobrevives en forma aislada, apenas te dejabas ver, mimetizándote en tu hábitat y al menor de los acechos, y aún así tu población huida…, hay que correr. se encuentra amenazada. Secreto de la naturaleza, Venado andino, huemul del norte, hoy te volvemos a ver La Taruca…. pero en paisaje precordillerano Silvestre, sensible, vulnerable dominio de faldeos grácil, robusto, esbelto, elegante, rocosos y escarpados, especie de conocer, admirar de pobre vegetación proteger y conservar, conformado. Taruca, Monumento Natural. 4 niñas y adultos, puedan identificar y apreciar las distintas especies con que contamos en la Región, y especialmente aquellas que más destacan y la hacen única.
    [Show full text]
  • Tagging Huemul Fawns (Hippocamelus Bisulcus) at Torres Del Paine National Park, Magallanes, Chile
    Anales Instituto Patagonia (Chile), 2016. Vol. 44(3):13-19 13 Tagging huemul fawns (Hippocamelus bisulcus) at Torres del Paine National Park, Magallanes, Chile Marcaje de cervatillos de Huemul (Hippocamelus bisulcus) en el Parque Nacional Torres del Paine, Magallanes, Chile Gladys Garay1, Oscar Guineo1 & Isaac M. Ortega3 Abstract como cervatillos resultó ser de suma importancia, There is no better approach in the study of social por lo tanto, se adoptó la técnica aprendida de behavior of a species than recognizing individuals. Franklin y Johnson (1994 ) para atrapar cervatillos For the similar looking animals as in the huemul durante la temporada de nacimientos. Equipos de (Hippocamelus bisulcus), were we could only investigadores buscaron y observaron hembras differentiate between sexes and ages, marking became de las que se sospechaba haber parido, para a critical need. In a long-term study of the huemul at así lograr la ubicación del cervatillo oculto. Una Torres del Paine National Park, Chile, we wanted to vez que se encontró el cervatillo se procedió a recognize individuals as young as fawns, hence we atraparlo, medirlo y marcarlo. De esta manera se adopted the technique learned from Franklin and atraparon, midieron y marcaron con auto-crotales Johnson (1994) to trap fawns during the birthing a 16 cervatillos. Estos animales marcados fueron season. Teams looked-up for females suspected to clave para una mejor comprensión de varios have given birth to find the location of the hidden aspectos de la ecología del huemul en ese parque. fawn. Once the fawn was located we proceeded to trap and mark it.
    [Show full text]
  • Redalyc.FEEDING ECOLOGY of the ENDANGERED HUEMUL
    Mastozoología Neotropical ISSN: 0327-9383 [email protected] Sociedad Argentina para el Estudio de los Mamíferos Argentina Vila, Alejandro R.; Galende, Gladys I.; Pastore, Hernán FEEDING ECOLOGY OF THE ENDANGERED HUEMUL (Hippocamelus bisulcus) IN LOS ALERCES NATIONAL PARK, ARGENTINA Mastozoología Neotropical, vol. 16, núm. 2, diciembre, 2009, pp. 423-431 Sociedad Argentina para el Estudio de los Mamíferos Tucumán, Argentina Available in: http://www.redalyc.org/articulo.oa?id=45712497014 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Mastozoología Neotropical, 16(2):423-431, Mendoza, 2009 ISSN 0327-9383 ©SAREM, 2009 Versión on-line ISSN 1666-0536 http://www.sarem.org.ar FEEDING ECOLOGY OF THE ENDANGERED HUEMUL (Hippocamelus bisulcus) IN LOS ALERCES NATIONAL PARK, ARGENTINA Alejandro R. Vila1, Gladys I. Galende2, and Hernán Pastore3 1 Wildlife Conservation Society, CC 794, (8400) Bariloche, Río Negro, Argentina. [Correspondence: A. Vila <[email protected]>]. 2 Departamento de Zoología, CRUB, Universidad Nacional del Comahue, Quintral 1250, (8400) Bariloche, Río Negro, Argentina. 3 CRUB, Universidad Nacional del Comahue - CONICET / DRP, Administración de Parques Nacionales, Vice Almirante O’Connor 1188, (8400) Bariloche, Río Negro, Argentina. ABSTRACT: The huemul (Hippocamelus bisulcus) is one of the most endangered and less known neotropical deer. We evaluated its diet in Los Alerces National Park, Argentina. Botanical composition of the diet was studied seasonally using microhistological analysis of fecal samples. Twenty six of the 72 available plant species were recorded in the huemul diet.
    [Show full text]
  • Cervidae Damián I
    MAMÍFEROS MEDIANOS Y GRANDES DE BOLIVIA CAPÍTULO24 Cervidae Damián I. Rumiz, Kathia Rivero, Humberto Gómez, Lila Sainz, Angela Nuñez & Robert B. Wallace Ciervo de los pantanos ( Blastocerus dichotomus) | Hermes Justiniano 631 CERVIDAE CAPÍTULO 24 CERVIDAE Damián I. Rumiz, Kathia Rivero, Humberto Gómez, Lila Sainz, Angela Nuñez & Robert B. Wallace DESCRIPCIÓN Y TAXONOMÍA Los cérvidos son la segunda familia más numerosa de los artiodáctilos modernos a nivel mundial (luego de Bovidae), con un total de 46 especies (Wilson & Reeder, 1993) y unas 18 en Latinoamérica (Weber & González, 2003). Los cérvidos tienen características morfológicas y fisiológicas que reflejan, en parte, un ancestro común y, en parte, adaptaciones al estilo de vida de un herbívoro, que está condicionado por una dieta difícil de digerir y por la necesidad de escapar de los depredadores (Putman, 1988). Su oído y olfato están extremadamente desarrollados. Aunque su vista tiene baja resolución más allá de los 60 m, tienen gran habilidad para detectar movimientos leves a mayores distancias. Paralelamente, su esqueleto y musculatura están adaptadas para huir rápidamente (Putman, 1988). 632 PARTE III DISTRIBUCIÓN Y ECOLOGÍA DE LOS MAMÍFEROS MEDIANOS Y GRANDES DE BOLIVIA Los cérvidos se caracterizan por tener dos dedos (III y IV) funcionales en cada extremidad, cubiertos por cascos o pezuñas, y pueden mantener dígitos carpales residuales (II y V) (Hershkovitz, 1982). Los machos desarrollan astas que caen y se regeneran periódicamente (en los renos Rangifer también las hembras). Las astas varían desde simples ( Pudu y Mazama ) o bifurcadas ( Hippocamelus ), hasta muy ramificadas ( Blastocerus , Odocoileus y Ozotoceros ), y son importantes en la competencia entre machos.
    [Show full text]