Anti- A2 (iPLA2) produced in rabbit, affinity isolated antibody

Product Number SAB4200129

Product Description Reagent Anti- (iPLA2) is produced in rabbit Supplied as a solution in 0.01 M phosphate buffered using as the immunogen a synthetic peptide saline, pH 7.4, containing 15 mM sodium azide. corresponding to a fragment of human iPLA2 (GeneID 8398), conjugated to KLH. The corresponding Antibody concentration: 1.5 mg/mL sequence is identical in mouse and rat iPLA2. The antibody is affinity-purified using the immunizing peptide Precautions and Disclaimer immobilized on agarose. For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for Anti-Phospholipase A2 (iPLA2) specifically recognizes information regarding hazards and safe handling human, mouse, and rat iPLA2. The antibody can be practices. used in several immunochemical techniques including immunoblotting (85 kDa and 95 kDa human iPLA2 and Storage/Stability 95 kDa mouse and rat iPLA2). Detection of the iPLA2 Store at −20 C. For continuous use, the product may bands by immunoblotting is specifically inhibited by the be stored at 2–8 C for up to one month. For extended iPLA2 immunizing peptide. storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not 2+ Ca -independent phospholipase A2 (iPLA2, also known recommended. If slight turbidity occurs upon prolonged as PLA2G6, INAD1, PARK14, PNPLA9) is a member of storage, clarify the solution by centrifugation. Discard the PLA2 superfamily that catalyzes the cleavage of working dilutions if not used within 12 hours. fatty acids from the sn-2 position of .1,2 PLA2 isoenzymes vary in their cellular localizations, Product Profile Ca2+ sensitivities, and substrate specificities. They Immunoblotting: a working antibody concentration of share the ability to catalyze the synthesis of precursors 1.5-3.0 g/mL is recommended using extracts of of proinflammatory mediators such as HEK-293T cells overexpressing human iPLA2, RAW264 and , through the release of arachidonic cell lysates, and rat pancreas extracts (S1 fraction). acid (AA) from membrane phospholipids. PLA2s play crucial roles in several cellular processes, including Note: In order to obtain best results in various intracellular membrane trafficking, differentiation, techniques and preparations, it is recommended to proliferation, and apoptosis. They are thought to play a determine optimal working dilutions by titration. role in oxidative and inflammatory responses in cerebral ischemia, Alzheimer’s disease (AD), and neuronal References 3 injury. iPLA2 group VIA comprises at least 5 1. Balsinde, J., and Balboa, M.A., Cell. Signal., 17, alternatively spliced isoforms. Isoforms LH-iPLA2 (90 1052-1062 (2005). kDa) and SH-iPLA2 (85 kDa). iPLA2 have been 2. Pontus, K.A., et al., Eur. J. Biochem., 262, 575-585 implicated in remodeling, nitric oxide- (1999). induced or -induced 3. Sun, G.Y., et al., J. Lipid Res., 45, 205-213 (2004). release, and in and 4. Morgan, N.V., et al., Nat. Genet., 38, 752-754 production. Mutations in the PLA2G6 are the (2006). cause of two childhood neurologic disorders, 5. Gregory, A., et al., Neurol., 71, 1402-1409 (2008). with iron accumulation (NBIA) 6. Schaeffer, E.L., and Gattaz, W.F., and infantile neuroaxonal dystrophy 1 (INAD1).4,5 Psychopharmacol., 198, 1-27 (2008). Recent evidence suggests both cPLA2 and iPLA2 may play a central role in memory deficits at early stages of VS,ER,AH,KAA,PHC,MAM 07/19-1 6 AD and in the AD neurodegenerative process.

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