Anti-Phospholipase A2 (iPLA2) produced in rabbit, affinity isolated antibody
Product Number SAB4200129
Product Description Reagent Anti-Phospholipase A2 (iPLA2) is produced in rabbit Supplied as a solution in 0.01 M phosphate buffered using as the immunogen a synthetic peptide saline, pH 7.4, containing 15 mM sodium azide. corresponding to a fragment of human iPLA2 (GeneID 8398), conjugated to KLH. The corresponding Antibody concentration: 1.5 mg/mL sequence is identical in mouse and rat iPLA2. The antibody is affinity-purified using the immunizing peptide Precautions and Disclaimer immobilized on agarose. For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for Anti-Phospholipase A2 (iPLA2) specifically recognizes information regarding hazards and safe handling human, mouse, and rat iPLA2. The antibody can be practices. used in several immunochemical techniques including immunoblotting (85 kDa and 95 kDa human iPLA2 and Storage/Stability 95 kDa mouse and rat iPLA2). Detection of the iPLA2 Store at −20 C. For continuous use, the product may bands by immunoblotting is specifically inhibited by the be stored at 2–8 C for up to one month. For extended iPLA2 immunizing peptide. storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not 2+ Ca -independent phospholipase A2 (iPLA2, also known recommended. If slight turbidity occurs upon prolonged as PLA2G6, INAD1, PARK14, PNPLA9) is a member of storage, clarify the solution by centrifugation. Discard the PLA2 superfamily that catalyzes the cleavage of working dilutions if not used within 12 hours. fatty acids from the sn-2 position of phospholipids.1,2 PLA2 isoenzymes vary in their cellular localizations, Product Profile Ca2+ sensitivities, and substrate specificities. They Immunoblotting: a working antibody concentration of share the ability to catalyze the synthesis of precursors 1.5-3.0 g/mL is recommended using extracts of of proinflammatory mediators such as prostaglandins HEK-293T cells overexpressing human iPLA2, RAW264 and leukotrienes, through the release of arachidonic cell lysates, and rat pancreas extracts (S1 fraction). acid (AA) from membrane phospholipids. PLA2s play crucial roles in several cellular processes, including Note: In order to obtain best results in various intracellular membrane trafficking, differentiation, techniques and preparations, it is recommended to proliferation, and apoptosis. They are thought to play a determine optimal working dilutions by titration. role in oxidative and inflammatory responses in cerebral ischemia, Alzheimer’s disease (AD), and neuronal References 3 injury. iPLA2 group VIA comprises at least 5 1. Balsinde, J., and Balboa, M.A., Cell. Signal., 17, alternatively spliced isoforms. Isoforms LH-iPLA2 (90 1052-1062 (2005). kDa) and SH-iPLA2 (85 kDa). iPLA2 have been 2. Pontus, K.A., et al., Eur. J. Biochem., 262, 575-585 implicated in phospholipid remodeling, nitric oxide- (1999). induced or vasopressin-induced arachidonic acid 3. Sun, G.Y., et al., J. Lipid Res., 45, 205-213 (2004). release, and in leukotriene and prostaglandin 4. Morgan, N.V., et al., Nat. Genet., 38, 752-754 production. Mutations in the PLA2G6 gene are the (2006). cause of two childhood neurologic disorders, 5. Gregory, A., et al., Neurol., 71, 1402-1409 (2008). neurodegeneration with brain iron accumulation (NBIA) 6. Schaeffer, E.L., and Gattaz, W.F., and infantile neuroaxonal dystrophy 1 (INAD1).4,5 Psychopharmacol., 198, 1-27 (2008). Recent evidence suggests both cPLA2 and iPLA2 may play a central role in memory deficits at early stages of VS,ER,AH,KAA,PHC,MAM 07/19-1 6 AD and in the AD neurodegenerative process.
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