Animal Health Report

Total Page:16

File Type:pdf, Size:1020Kb

Animal Health Report 2006 UNITED STATES Animal Health Report United States Department of Agriculture Animal and Plant Health Inspection Service Agriculture Information Bulletin No. 801 The U.S. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual’s income is derived from any public assistance program. (Not all prohibited bases apply to all programs.) Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA’s TARGET Center at (202) 720–2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250–9410, or call (800) 795–3272 (voice) or (202) 720–6382 (TDD). USDA is an equal opportunity provider and employer. Mention of companies or commercial products does not imply recommendation or endorsement by the USDA over others not mentioned. USDA neither guarantees nor warrants the standard of any product mentioned. Product names are mentioned solely to report factually on available data and to provide specific information. Issued September 2007 U.S. Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services Centers for Epidemiology and Animal Health NRRC Building B, Mailstop 2E7 2150 Centre Avenue Fort Collins, CO 80526–8117 (970) 494–7000 http://www.aphis.usda.gov/publications Foreword We are pleased to present the 2006 annual report on of the animal health infrastructure but also provide the status of animal health in the United States. This a foundation to promote discussion and exchange of is the third such report that provides a wide-ranging knowledge, both internationally and domestically, on review of the health of our Nation’s domestic animal these important research areas. resources. We also dedicate a chapter (chapter 9, page 105) The report highlights significant epidemiologic to APHIS-wide international collaboration and events of 2006 and provides insight into the Nation’s capacity-building projects. We in Veterinary animal health surveillance activities. In addition, the Services, and APHIS employees in other units, are report presents an update on programs, both new proud of the roles we play in numerous training, and existing, that strive to maintain healthy livestock, education, and outreach programs under way poultry, and aquaculture populations. throughout the world to safeguard and improve This year, we are introducing two new animal and human health globally. chapters—animal health research and international I believe you will find the 2006 Animal Health collaboration. Animal health research is an important Report a helpful resource on the status of U.S. component of the U.S. animal health infrastructure. livestock, poultry and aquaculture, as well as A description of this infrastructure—a complex programs and strategies that are in place to ensure network of Federal, State, and industry partners— their continued health. As always, I invite and is again included in the report as a reference tool. encourage your comments and ideas, as well as Chapter 8 (page 97) presents additional information suggestions on how we can improve next year’s on some of the research under way by agencies of the report. You will find information on how to provide U.S. Department of Agriculture (USDA) and America’s feedback and contact details on page 159. Thank you schools of veterinary medicine. We hope this new for reading. chapter will not only enhance your understanding — John Clifford Deputy Administrator, Veterinary Services USDA–APHIS Washington, DC i Contents Chapter 1: Animal Health Events in 2006 . 1 BSE Case in Alabama . 1 Vesicular Stomatitis . 1 Anthrax . 2 Viral Hemorrhagic Septicemia . 3 Honey Bee Colony Collapse Disorder . 3 Contagious Equine Metritis . 4 Equine Herpesvirus Type 1 . 5 Equine Viral Arteritis . 6 Chapter 2: Animal Health Initiatives . 9 NAIS . 9 The “NAIS Draft User Guide” . 9 NAIS Program Update . 9 NAIS Outreach . 10 NVAP . 11 Chapter 3: National Animal Health Surveillance System (NAHSS) . 15 Program Disease Surveillance . 16 FAD Surveillance and Programs . 16 FAD Surveillance and Investigations . 16 Surveillance in Disease-Control Programs . 17 New Surveillance Plans and Evaluations . 24 Surveillance Reporting . 26 Emerging Diseases and Issues . 28 Identification and Tracking of Emerging Animal Health Issues . 29 Selected Domestic Emerging Issues in 2006 . 30 Monitoring Activities (NAHSS) . 31 Swine 2006 Study . 32 Equine 2005 Part II: Changes in the U .S . Equine Industry, 1998–2005 Report . 33 iii Equine Events 2005 . 34 Determining U .S . Milk Quality Using Bulk Tank Somatic Cell Counts, 2006 . 36 Sentinel Feedlot Surveillance . 38 Chapter 4: Animal Disease Eradication Programs and Control and Certification Programs . 41 Eradication Programs . 41 Scrapie in Sheep and Goats . 41 Tuberculosis (TB) in Cattle and Cervids . 44 Pseudorabies in Swine . 47 Brucellosis in Swine . 48 Brucellosis in Cattle and Bison . 49 Control and Certification Programs . 52 Chronic Wasting Disease (CWD) in Cervids . 52 Johne’s Disease in Cattle . 54 Trichinae in Swine . 55 Swine Health Protection Inspection Program . 57 Chapter 5: Emergency Management and Response . 59 Prevention Methods . 59 AHE Response . 60 Overview . 60 NAHEMS Topics . 61 Structure of Emergency Management System . 61 Emergency Management Activities and Accomplishments in 2006 . 62 NVS . 62 Background . 62 NVS Achievements in 2006 . 63 National Animal Health Laboratory Network (NAHLN) . 64 NAHLN Achievements in 2006 . 64 Chapter 6: Animal Trade . 69 Background . 69 Export (Domestic) . 72 Trade Restrictions Due to Avian Influenza (AI) . 72 Import Trade Rules in 2006 . 73 iv 2006 United States Animal Health Report Chapter 7: Overview of U.S. Livestock, Poultry, and Aquaculture Production in 2006 . 75 Available Statistics . 75 Number of Farms . 75 Relative Magnitude of Industries by Value of Production . 77 Introduction to the Livestock, Poultry, and Aquaculture Industries . 78 Cattle and Calves (Beef and Dairy) . 78 Milk Cows—Dairy . 80 Beef Cows . 81 Cattle on Feed . 83 Hogs . 84 Sheep and Goats . 86 Poultry Industries . 88 Equine Industry . 91 Fish and Other Aquaculture Products . 91 Honey Production . 94 Miscellaneous . 94 Number of Livestock Slaughter Plants in the United States . 94 Chapter 8: Research Initiatives in Animal Health . 97 Introduction . 97 Federal Research Responsibilities . 97 ARS . 97 CSREES . 99 ERS . 100 NASS . 101 Research Programs Associated With University and College Programs in Veterinary Medicine . 101 Chapter 9: Collaboration and International Capacity-Building Efforts . 105 World Organization for Animal Health (OIE) Collaborations . 105 International AI Assistance . 108 v Appendix 1—Statistics on Major Commodities . 111 Appendix 2—Tables on FAD Investigations . 125 Appendix 3—Animal Health Infrastructure in the United States . 135 Appendix 4—Animal Health Contacts in the United States . 145 Appendix 5—Key U.S. Animal Health Web Sites . 151 Appendix 6—Acronyms and Abbreviations . ..
Recommended publications
  • Whole-Genome Sequencing for Tracing the Genetic Diversity of Brucella Abortus and Brucella Melitensis Isolated from Livestock in Egypt
    pathogens Article Whole-Genome Sequencing for Tracing the Genetic Diversity of Brucella abortus and Brucella melitensis Isolated from Livestock in Egypt Aman Ullah Khan 1,2,3 , Falk Melzer 1, Ashraf E. Sayour 4, Waleed S. Shell 5, Jörg Linde 1, Mostafa Abdel-Glil 1,6 , Sherif A. G. E. El-Soally 7, Mandy C. Elschner 1, Hossam E. M. Sayour 8 , Eman Shawkat Ramadan 9, Shereen Aziz Mohamed 10, Ashraf Hendam 11 , Rania I. Ismail 4, Lubna F. Farahat 10, Uwe Roesler 2, Heinrich Neubauer 1 and Hosny El-Adawy 1,12,* 1 Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, 07743 Jena, Germany; AmanUllah.Khan@fli.de (A.U.K.); falk.melzer@fli.de (F.M.); Joerg.Linde@fli.de (J.L.); Mostafa.AbdelGlil@fli.de (M.A.-G.); mandy.elschner@fli.de (M.C.E.); Heinrich.neubauer@fli.de (H.N.) 2 Institute for Animal Hygiene and Environmental Health, Free University of Berlin, 14163 Berlin, Germany; [email protected] 3 Department of Pathobiology, University of Veterinary and Animal Sciences (Jhang Campus), Lahore 54000, Pakistan 4 Department of Brucellosis, Animal Health Research Institute, Agricultural Research Center, Dokki, Giza 12618, Egypt; [email protected] (A.E.S.); [email protected] (R.I.I.) 5 Central Laboratory for Evaluation of Veterinary Biologics, Agricultural Research Center, Abbassia, Citation: Khan, A.U.; Melzer, F.; Cairo 11517, Egypt; [email protected] 6 Sayour, A.E.; Shell, W.S.; Linde, J.; Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Elzera’a Square, Abdel-Glil, M.; El-Soally, S.A.G.E.; Zagazig 44519, Egypt 7 Veterinary Service Department, Armed Forces Logistics Authority, Egyptian Armed Forces, Nasr City, Elschner, M.C.; Sayour, H.E.M.; Cairo 11765, Egypt; [email protected] Ramadan, E.S.; et al.
    [Show full text]
  • Syndromic Surveillance for Zoonotic Diseases
    USING PRE-DIAGNOSTIC DATA FROM VETERINARY LABORATORIES TO DETECT DISEASE OUTBREAKS IN COMPANION ANIMALS DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By Loren Eldon Shaffer, MPH ***** The Ohio State University 2007 Dissertation Committee: Approved by Professor William J.A. Saville, Advisor Professor Julie Funk Professor Päivi Rajala-Schultz ______________________________________ Advisor Professor Michael M. Wagner Graduate Program in Veterinary Preventive Medicine Professor Thomas E. Wittum ABSTRACT Emerging infectious diseases and the threat of bioterrorism have fostered a desire for improved timeliness of outbreak detection. Traditional disease reporting is reliant on confirmed diagnoses, often involving laboratory analysis that may require days to complete. Most emerging infectious and bioweapon pathogens are zoonotic organisms. Detection of zoonotic outbreaks has often relied on the identification of human cases. We investigated how data from veterinary diagnostic laboratories (VDLs) might contribute to earlier outbreak detection efforts in Ohio. We began by determining the representation of animal species in the data and evaluating the representation of human households. Companion animals comprised 98.1% of the total number of specimens submitted to a commercial, nation-wide VDL from clinics in Ohio in one year. Using estimates derived from a survey of pet owners, we determined that these data represented approximately 6.6% of Ohio households. The value of microbiology test orders was determined by quantifying the representation and potential gain in timeliness from two VDL datasets. We also investigated the potential to determine estimated count values from historical records and detect significant increases in these values using statistical-based detection methods.
    [Show full text]
  • Ovine Brucellosis J
    INFECTION AND IMMUNITY, Feb. 1990, p. 489-494 Vol. 58, No. 2 0019-9567/90/020489-06$02.00/0 Copyright C 1990, American Society for Microbiology Antibody Response to Brucella ovis Outer Membrane Proteins in Ovine Brucellosis J. I. RIEZU-BOJ,1 I. MORIYON,1* J. M. BLASCO,2 C. GAMAZO,1 AND R. DIAZ' Departamento de Microbiologia, Universidad de Navarra, Apartado 273, 31080 Pamplona,' and Departamento de Produccion Animal, Servicio Investigaci6n Agraria, Diputaci6n General Aragon, 50080 Zaragoza, Spain Received 20 March 1989/Accepted 20 October 1989 Downloaded from Hot saline extracts of Brucella ovis were composed of vesicles with outer membrane proteins (OMPs), lipopolysaccharide, and phospholipid as constituents. Extraction with petroleum ether-chloroform-phenol yielded a protein fraction free of detectable lipopolysaccharide, in which group 3 OMPs (28,500 apparent molecular weight [28.5K], 27.0K, and 25.5K) represented 81% of the total. Group 1 OMPs and 67.0K, 22.5K to 21.5K, and 19.5K to 18.0K proteins were also detected. Adsorption of immune sera with whole bacteria suggested that group 3 OMPs and 67.0K, 22.5K to 21.5K, and 19.5K to 18.0K proteins had antigenic determinants exposed on the surfaces of both B. ovis and rough B. melitensis cells but not on smooth B. melitensis cells. Antibodies to group 3 OMPs and the 67.0K protein in the sera of 93 and 87%, respectively, of http://iai.asm.org/ B. ovis-infected rams were found by immunoblotting. Antibodies to other proteins were present in 67% of these animals. Compared with B.
    [Show full text]
  • Estudio De Brucelosis Causada Por Brucella Ovis En Ovinos TESIS DOCTORAL Gustavo Aldo Lopez
    UNIVERSIDAD POLITÉCNICA DE VALENCIA DEPARTAMENTO DE CIENCIA ANIMAL Estudio de Brucelosis causada por Brucella ovis en ovinos TESIS DOCTORAL Gustavo Aldo Lopez Directora: Dr. Nidia Elena Lucero Valencia, 2007 1 El presente trabajo de Tesis ha sido realizado en la Facultad de Ciencias Agrarias de la Universidad Nacional de Lomas de Zamora y en el Laboratorio de Brucelosis del Instituto Nacional de Enfermedades Infecciosas-Administración Nacional de Laboratorios e Institutos de Salud, “Dr. C.G. Malbran” (INEI-ANLIS). Argentina. 2 Esta tesis ha sido presentada como uno de los requisitos para optar al grado de Doctor por la Universidad Politécnica de Valencia. El doctorando El director de tesis Fdo. Gustavo Aldo Lopez Fdo. Nidia Elena Lucero 3 AGRADECIMIENTOS Deseo agradecer a las personas e instituciones que contribuyeron con su aporte material y anímico a la finalización de esta Tesis. A mi directora, Dra. Nidia Lucero. A la Directora del INEI-ANLIS, Dra. Marìa Inés De Mitri Al personal del laboratorio de brucelosis del INEI-ANLIS, en especial a las Técnicas Químicas Sandra M. Ayala y Déborah B. Hasan y a la Bioq. Gabriela I. Escobar. Al Dr. Fernando Paolicchi, Rosana Malena y Andrea Florentino, del laboratorio de Bacteriología del INTA-Balcarce-Bs.As., por su colaboración con muestras de ovinos. A mis compañeros de facultad. Al Dr. Manuel Láinez, quien gentilmente aceptó ser mi tutor en Valencia. Al Dr. Salvador Vicente, por su invalorable colaboración. A las autoridades de las Facultades de Cs. Agrarias e Ingeniería. A las autoridades de la UPV. Al Dr. Enrique Frank. Especialmente a Patri, Agu, Pato, Mai y Sebi, por su paciencia y comprensión.
    [Show full text]
  • A Review of Brucellosis: a Recent Major Outbreak in Lebanon
    J Environ Sci Public Health 2021;5 (1):56-76 DOI: 10.26502/jesph.96120117 Review Article A Review of Brucellosis: A Recent Major Outbreak in Lebanon Alia Sabra1*, Bouchra el Masry2, Houssam Shaib2* 1One Health Program, Duke University, Durham, North Carolina, USA 2Department of Agriculture, Faculty of Agricultural and Food Sciences, American University of Beirut, Beirut, Lebanon *Corresponding Author: Houssam Shaib (PhD), Faculty of Agricultural and Food Sciences, American University of Beirut, Riad El Solh 1107-2020, PO Box 11-0236, Beirut, Lebanon, Tel: +961-1-350000; E-mail: [email protected] Alia Sabra, (MS in Environment, MS in Energy, Certificate in One Health), One Health Program, Duke University, Durham, North Carolina, E-mail: [email protected] Received: 08 January 2021; Accepted: 16 January 2021; Published: 11 February 2021 Citation: Alia Sabra, Bouchra el Masry, Houssam Shaib. A Review of Brucellosis: A Recent Major Outbreak in Lebanon. Journal of Environmental Science and Public Health 5 (2021): 56-76. Abstract with the Lebanese alimentary habits, brucellosis is Brucella infection remains the world’s most common commonly diagnosed in adults aged between 20 and bacterial zoonosis, with over half a million new cases 60 years old. This paper tailors the first annually, which brought renewed attention of this comprehensive One Health approach for the control neglected disease. This attention is highlighted in this of Brucellosis in Lebanon. Herein, a broad review to review manuscript, reporting worldwide outbreaks shed light on the complexity of Brucellosis and introducing the 5th major severely prominent discussing: the etiology; taxonomy; pathogenesis; worldwide outbreak since 2016 which occurred in epidemiology and geographic distribution of the Lebanon.
    [Show full text]
  • Brucellosis in Sheep and Goats and Its Serodiagnosis and Epidemiology
    Int.J.Curr.Microbiol.App.Sci (2018) 7(1): 1848-1877 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 01 (2018) Journal homepage: http://www.ijcmas.com Review Article https://doi.org/10.20546/ijcmas.2018.701.225 Brucellosis in Sheep and Goats and its Serodiagnosis and Epidemiology Neharika Saxena1*, Balbir Bagicha Singh1 and Hari Mohan Saxena2 1School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India 2Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India *Corresponding author ABSTRACT Brucellosis is an important zoonotic disease globally that causes huge economic losses to the livestock owners and is of public health significance. Brucellosis in animals is endemic K e yw or ds in India. In sheep and goats, Brucellosis is mainly caused by Brucella melitensis whereas Brucella ovis causes the disease in sheep. The symptoms in infected sheep and goats are Ovine Brucellosis, abortions, stillbirths and the birth of weak offsprings. Animals that abort may retain the Caprine Brucellosis, Serodiagnosis , placenta. Sheep and goats usually abort only once, but reinvasion of the uterus and Seroepidemiology shedding of organisms can occur during subsequent pregnancies. Several studies have been carried out on seroepidemiology of caprine and ovine Brucellosis in India. The tests Article Info commonly used for diagnosis of Brucellosis are the milk ring test, Rose Bengal Plate Test (RBPT), Standard Tube Agglutination Test (STAT), Microtiter Plate Agglutination Test Accepted: (MAT) and ELISA. The RBPT is a rapid screening test for the diagnosis of Brucellosis.
    [Show full text]
  • Ccommittee on Revisit Board on Div Ting Bruce N Agricultu Vision on E
    Committee on Revisiting Brucellosis in the Greater Yellowstone Area Board on Agriculture and Natural Resources Division on Earth and Life Studies A Report of THE NATIONAL ACADEMIES PRESS 500 Fifth Street, NW Washington, DC 20001 This study was supported by Grant #14-9795-2254-GR between the National Academy of Sciences and the U.S. Department of Agriculture Animal and Plant Health Inspection Service. Any opinions, findings, conclusions, or recommendations expressed in this publication do not necessarily reflect the views of any organization or agency that provided support for the project. International Standard Book Number-13: 978-0-309-45831-3 International Standard Book Number-10: 0-309-45831-5 Digital Object Identifier: https://doi.org/10.17226/24750 Additional copies of this publication are available for sale from the National Academies Press, 500 Fifth Street, NW, Keck 360, Washington, DC 20001; (800) 624-6242 or (202) 334-3313; http://www.nap.edu. Copyright 2017 by the National Academy of Sciences. All rights reserved. Printed in the United States of America Cover photo courtesy of Mark Gocke, Wyoming Game and Fish Department. Suggested citation: National Academies of Sciences, Engineering, and Medicine. 2017. Revisiting Brucellosis in the Greater Yellowstone Area. Washington, DC: The National Academies Press. doi: https://doi.org/10.17226/24750. The National Academy of Sciences was established in 1863 by an Act of Congress, signed by Presi- dent Lincoln, as a private, nongovernmental institution to advise the nation on issues related to sci- ence and technology. Members are elected by their peers for outstanding contributions to research. Dr.
    [Show full text]
  • Brucellosis in Humans and Livestock in Rural Uganda: Epidemiology and Tools for Resource-Limited Settings
    BRUCELLOSIS IN HUMANS AND LIVESTOCK IN RURAL UGANDA: EPIDEMIOLOGY AND TOOLS FOR RESOURCE-LIMITED SETTINGS By RoseAnn Miller A DISSERTATION Submitted to Michigan State University in partial fulfillment of the requirements for the degree of Large Animal Clinical Sciences – Doctor of Philosophy 2016 ABSTRACT BRUCELLOSIS IN HUMANS AND LIVESTOCK IN RURAL UGANDA: EPIDEMIOLOGY AND TOOLS FOR RESOURCE-LIMITED SETTINGS By RoseAnn Miller Objectives: A cross-sectional study was undertaken to describe the epidemiology of zoonotic brucellosis in dairy farms in rural southwestern Uganda, and to evaluate the use of tools to address challenges to disease surveillance programs faced in rural sub-Saharan Africa and other resource-limited settings. Methods: Blood samples were collected from cattle, goats and humans, and milk samples were collected from cows on 70 dairy farms in two sites in Uganda. Samples of blood and milk from livestock were collected and dried on laboratory-grade filter paper. Data describing each animal and human subject were recorded during sample collection, and herd-level risk factor data were collected by questionnaires on livestock health and management, human health and practices associated with increased brucellosis risk. Livestock blood and milk samples were tested using the Rose Bengal (RBT) and milk ring (MRT) tests. Human blood was tested using an immunochromatographic lateral flow assay (LFA). A qualitative real-time PCR (q-PCR) and nested PCR (n-PCR) were used to detect Brucella in DNA extracted from human, cattle and goat blood clots, cow milk, and human sera, and q-PCR was used to test DNA extracted from cattle and goat dried blood and milk samples.
    [Show full text]
  • The Epidemiology of Brucellosis in Sheep, Goats and Humans in the Iraqi Kurdistan Region
    The Epidemiology of Brucellosis in Sheep, Goats and Humans in the Iraqi Kurdistan Region Emad Abdlghafoor Aziz Alshwany (BVMS, HDipVM/Poultry Diseases & MVS) A thesis presented in fulfilment of the requirements for the degree of Doctor of Philosophy School of Veterinary Medicine College of Sciences, Health, Engineering and Education Murdoch University Western Australia 2019 Declaration I declare that this thesis is my own account of my research and contains as its main content work which has not previously been submitted for a degree at any tertiary education institution. Emad Alshwany ii Dedication I dedicate this Doctoral Thesis to the most precious people in my life, for their everlasting love and strong support in my study: My parents, wife, daughters, son, sister and brothers. iii Abstract Brucellosis is a disease affecting a wide range of domesticated animals and wildlife as well as humans. The disease remains a major zoonotic problem in many regions including the Middle East. In Iraq, where brucellosis is endemic, the disease is a major economic and production limiting disease for livestock owners and the community. Impacts on production arise from reduced milk production, abortions, decreased reproduction rate and premature births. The aim of this project was to investigate the seroprevalence, risk factors and economic impact of brucellosis in sheep and goats in the Kurdistan Region. Also a retrospective study of human brucellosis in Iraq was conducted to describe the historical distribution of the disease and its impact on the population. Fifty one (39 sheep and 12 goats) of 1,050 sera samples were positive on both an RBT and ELISA (overall seroprevalence of 4.9%; 95%CI 3.6 - 6.3).
    [Show full text]
  • The CO2-Dependence of Brucella Ovis and Brucella Abortus Biovars Is Caused by Defective Carbonic Anhydrases
    Pérez‑Etayo et al. Vet Res (2018) 49:85 https://doi.org/10.1186/s13567-018-0583-1 RESEARCH ARTICLE Open Access The ­CO2‑dependence of Brucella ovis and Brucella abortus biovars is caused by defective carbonic anhydrases Lara Pérez‑Etayo1, María Jesús de Miguel2, Raquel Conde‑Álvarez1, Pilar M. Muñoz2, Mammar Khames3,4, Maite Iriarte1, Ignacio Moriyón1 and Amaia Zúñiga‑Ripa1* Abstract Brucella bacteria cause brucellosis, a major zoonosis whose control requires efcient diagnosis and vaccines. Iden‑ tifcation of classical Brucella spp. has traditionally relied on phenotypic characterization, including surface antigens and 5–10% ­CO2 necessity for growth ­(CO2-dependence), a trait of Brucella ovis and most Brucella abortus biovars 1–4 strains. Although molecular tests are replacing phenotypic methods, ­CO2-dependence remains of interest as it condi‑ tions isolation and propagation and refects Brucella metabolism, an area of active research. Here, we investigated the connection of ­CO2-dependence and carbonic anhydrases (CA), the enzymes catalyzing the hydration of CO­ 2 to the bicarbonate used by anaplerotic and biosynthetic carboxylases. Based on the previous demonstration that B. suis car‑ ries two functional CAs (CAI and CAII), we analyzed the CA sequences of ­CO2-dependent and -independent brucellae and spontaneous mutants. The comparisons strongly suggested that CAII is not functional in CO­ 2-dependent B. abor- tus and B. ovis, and that a modifed CAII sequence explains the ­CO2-independent phenotype of spontaneous mutants. Then, by mutagenesis and heterologous plasmid complementation and chromosomal insertion we proved that CAI alone is enough to support ­CO2-independent growth of B. suis in rich media but not of B.
    [Show full text]
  • Brucella Ovis
    Vizcaíno et al. Vet Res (2020) 51:101 https://doi.org/10.1186/s13567-020-00824-7 RESEARCH ARTICLE Open Access Disruption of pyruvate phosphate dikinase in Brucella ovis PA CO2-dependent and independent strains generates attenuation in the mouse model Nieves Vizcaíno1,2, Lara Pérez‑Etayo3, Raquel Conde‑Álvarez3, Maite Iriarte3, Ignacio Moriyón3 and Amaia Zúñiga‑Ripa3* Abstract Brucella ovis is a non‑zoonotic rough Brucella that causes genital lesions, abortions and increased perinatal mortal‑ ity in sheep and is responsible for important economic losses worldwide. Research on virulence factors of B. ovis is necessary for deciphering the mechanisms that enable this facultative intracellular pathogen to establish persistent infections and for developing a species‑specifc vaccine, a need in areas where the cross‑protecting ovine smooth B. melitensis Rev1 vaccine is banned. Although several B. ovis virulence factors have been identifed, there is little information on its metabolic abilities and their role in virulence. Here, we report that deletion of pyruvate phosphate dikinase (PpdK, catalyzing the bidirectional conversion pyruvate ⇌ phosphoenolpyruvate) in B. ovis PA (virulent and CO2‑dependent) impaired growth in vitro. In cell infection experiments, although showing an initial survival higher than that of the parental strain, this ppdK mutant was unable to multiply. Moreover, when inoculated at high doses in mice, it displayed an initial spleen colonization higher than that of the parental strain followed by a marked comparative decrease, an unusual pattern of attenuation in mice. A homologous mutant was also obtained in a B. ovis PA CO 2‑independent construct previously proposed for developing B.
    [Show full text]
  • Brucella Melitensis
    10 10 ISSN 1810-0732 FAO ANIMAL PRODUCTION AND HEALTH Brucella melitensis infection is recognized as a significant public health challenge, with a major economic and financial burden in countries where the disease remains endemic. In Eurasia and the Middle East Brucella melitensis Brucella melitensis infections in sheep and goats are still widespread, resulting in significant human illness, primarily from consumption of contaminated dairy products or from occupational exposure to infected livestock. In small ruminants (sheep and goats), abortion, reduced fertility, reduced milk production and lowered newborn viability are the major impacts. There are very significant benefits to human health and poverty alleviation from controlling and eradicating B. melitensis infections in animals and the Food and Agriculture Organization of the United Nations (FAO) has been responsible for advancing practical knowledge and experience on brucellosis in various in Eurasia and the Middle East countries and assisting in the development of sound strategies and policies for sustainable control programmes. As part of these efforts a technical meeting of proceedings brucellosis experts was convened in Rome from 11 to 14 May 2009 by the FAO in collaboration with the World Health Organization (WHO) and the World Organisation for Animal Health (OIE), in order to develop further guidance to support and improve surveillance and control of Brucella melitensis infection in affected countries. This document provides an account on the objectives, discussions and outcomes of the meeting and provides an up to date account of the available options for the prevention and control of B. melitensisi as well as the identified gaps that still need to be addressed.
    [Show full text]