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FAST TOXICOLOGICAL SCREENING KIT BY LC-MS/MS – Code SC9000B

The kit for the toxicologicalforensic analysis is a screening method to analyze hundreds of compounds in positive and negative mode, in plasma and blood. It is possible to determine the following molecules:

POSITIVE MODE: Amitrole Alypin Bamifylline 17 alpha Methyltestosterone Amantadine Bamipin 2Amino 5chlorobenzophenone Ambroxol Beclamide 2Amino 5nitrobenzophenone Ambucetamide Beclometasone dipropionate 2Hydroxyethylflurazepam Amezinium 3,4 Methylenedioxyamphetamine Amidopyrin Bendiocarb 3,4 Methylenedioxyethylamphetamine Amidotrizoic Acid Benfluorex 3,4 Methylenedioxymethamphet amine Amiloride Benodanil 3,5 Diiodotyrosine Aminodantrolene Benperidole 4Benzamidosalicyclic acid Aminoglutethimide Benproperine 6Mercaptourine Aminophenazone Bensultap 6OMonoacetylmorphine Aminopromazine Bentiromide 7Aminoclonazepam Aminorex Benzatropine 7Aminodesmethylflunitrazepam Amiodarone Benzocaine 7Aminoflunitrazepam Amiphenazole Benzoct amine 7Aminonitrazepam Amisul pride Benzododecinium 9Hydroxyrisperidone Amitriptylin Benzoylecgonine cebutolol Amlodipine Benzthiazuron Ammoidin Berberine Aceclofenac Amorolfine Betamethasone 21 phosphate Acemetacin Amoxicillin Acep romazine Amphetamine Bevonium Aceprometazine Amrinone Bezafibrate Acetiamin Antazoline Biperiden Acetylaminonitroprophoxybenzene Apalcillin Aciclovir Bornaprine Actinoquinol Apophedrin Brodifacoum Adenine Apraclonidin Bromacil Adrenalone Aprinidine Bromazepam Ajmaline Alachlor Atorvastatin Brompheniramine Alclometasone 17,21 dipropionate Atraton Brotizolam Alimemazine Atrazine desethyl Bucetin Alizapride Bufe xamac Allopurinol Atropinmethylbromid Bumetanide Almitrine Az atadi ne alpha Hydroxyalprazolam Azelastine alpha Hydrox ytriazolam Azintamid Bupivacaine Alprazolam Aztreonam Baclofen Buprenorphine Altretamine Buprofezin

1 Clobenzepam Dienogest Clobutino l Diethazine Butetamate Cl omethiazole Diethylcarbamazine Butoxycaine Difenoconazole Cafaminol Clonazepam Difenoxuron Caffeine Diflucortolone Candesartan Dihydrocodeine Caproylresorcinol Clopidogrel Dihydroergocristin Captopril Cloprednol Dihydr oergotamine Dilazep Carbamazepine Cocaine Diltiazem Carbamazepine 10,11 ep oxide Codeine Dimetindene Carbendazim Coniine Dimetotiazine Carbinoxamine Corticosterone Dimetridazole Carbutamide Cortisone Dioxethedrin Cotinine Diphenamid Coumatetralyl Diphenhydramine Croconazole Diponium Cromoglicic acid Diprophylline Cerivastatin Dipyridamole Cetirizine Cyclicine Disopyramide Chinine Cyclophosphamide Dixyrazine Chlorazanil Cycl ovalone Chlorbenzoxamine Chlorcyclizine Cytarabine Doxapram Chlordiazepoxide D3 Doxepin Chloridazon D5 Diazepam Doxylamine Chlormadinone Acetate Drofenine Chlormequat Deflazacort Dummy Chlorpheneth iazin e Demeton Smethylsulfone Ecgoninemethylester Chlorpheniramine Denaverine EDDP Desalkylflurazepam Embutramide Chlorpromazine Sulfoxide Enalapril Desmedipham Enoximone Cilastatin Desmethylclobazam Cilazapril Desmethylclomipramine Eprosartan Cimetidine Esculin Cinnarizine Dexamethasone 21 isonic otina te Cinoxacin Dexfenfluramine Estazolam Ciprofloxacin Dextromethorphan Ethenzamide Cisapride Dextropropoxyphene Etofenamate Citalopram Diaveridine Etomidate Clarithromycin Diazepam Etoposide Clemastine Dibutyl adipate Famotidine Diclofenac Fedrilate Clobazam Dicycloverine Felbamate

2 Felodipine Guanoxan Lisinopril Fenarimol Lonazolac Fendiline Haloxyfop ethoxyethyl ester Loperamide Fenetylline Heptaminol Loratadine Fenflu ramin e Heroin Lorazepam Fenpipramide Hexazinone Lormetazepam Fenpiprane Hexobendine Losartan Fenproporex Histamine Fentanyl Histapyrrodine Lysergide Fenticonazole Homatropine Mafenide Fexofenadine Hordenine Mapro tilin e Flecainide Hydrocodone MBDB Flocoumafen Hydrocortisone Mebeverine Floctafenine Hydrocortisone 21 acetate Meclizine Fluanisone Hydromorphone Meclofenamic acid Fluconazole Hydr oxyzine Mecloxamine Flumazenil Hymecromone Meclozine Flunarizine Medazepam Flunitrazepam Indanazoline Mefenamic acid Fluoxetine Indapamine Mefenorex Indinavir Mefexamide Indoprofen Mefloquine Flupirtine Melatonin Flurazepam Iopodic acid Flu rochl oridone Ipratropium Meloxicam Fluspirilen Iprazochrome Fluticasone Propionate Irbesartan Melphalan Fluvastadine Isoaminile Fluvoxamine Isoconazole Mepivacaine Fuberidazole Meptazinol Furalaxyl Isoproturon Mequitazine Gabapentin Isothipendyl Mescalin e Galantamine Isoxicam Me soridazine Gallopamil Mesuximide Gemcitabine Kavain Metaclazepam Glibenclamide Ketamin e Metamfepramone Glibornuride Ke toprofen Metamitron Gliclazide Ketorolac Metamphetamine Glimepiride Ketotifen Metazachlor Glipizide Lamotrigine Metenolone acetate Gliquidone Lercanidipine Metformin Griseofulvin Levocabastine Methabenzthiazuron Guaifenesin Levodopa Methadone Guan abenz Methaphenilene Levopropylhexedrin Methapyrilene Lidocaine Methaqualon

3 Methfuroxam Nefopam Oxitriptan Methocarbamol Nicardipine Oxitropium Methopr otryn e Nicotinamide Oxomemazine Methotrexate Nicotine Methylephedrine Nifedipine Oxybuprocaine Methylphenidate Nifenazone Oxybutynin Methylscopolamine Niflumic acid Oxyco done Methylthiouracil Nimodipine Nimorazole Metixene Nisoldipine Oxymorphon NIsopropylsalicylamide Oxypertine Nitrazepam Papaverine Metsulfuron methyl Nitrendipine Paracetamol Metyrapone Nizatidi ne Mexiletine NMethylephedrine Paroxetine Nomifensine Penfluridol Norbuprenorphine Pentamidine Midazolam Nordiazepam Pentoxyverine Nordiazepam N ethyl Milrinone Norephedrine Minoxidil Norethisterone Perindopril Mirtaz apine Norethisterone acetate Mizolastine Norfenefrine Pethidine Moclobemide Norfentanyl Phenacetin Modafinil Norfloxacin Phenazone Molsidomine Normorphine Phenazopyridine Monocrotophos Phencyclid ine Monolinuron Noscapine Phenelzine Moperone Nuarimol Pheniramine Morphine Obidoxime Phenprocoumon Morphine 3ßDglucuronide Ofloxacin Moxaverine Ola nzapi ne Phenyltoloxamine Ondansetron Phenytoin Moxonidine Pholedrine Nabumetone Ornidazole Naftidrofuryl Orphenadrine Naftifine Oxadixyl Pioglitazone Nalidixic acid Oxamyl Nalorphine Oxatomide Naloxon e Oxazepam Pirenzepine Na ltrexone Oxcarbazepine Piretanide Nandrolone Oxeladin Piritramide Oxetacaine Piroxicam NDespropylpropafenone Oxiconazole

4 Tetroxoprim Prajmal ium Pramipexole Rizatriptan THC Prazepam Ropinirole THC COOH Ropivacaine THC OH Prednisolone Rosiglitazon Thebacon Prednisone Theobromine Prilocaine Salicylamide Theophylline Primaquine Scopolamine Thiabendazole Primidone Serotonin Thiamazole Procainamide Thiamine Sertraline Thiopr opaza te Procyclidine Sildenafil Thioproperazine Progesterone Simazine Thiram Promethazin Spirapril Tiagabine Prometryn Stano zolol Tiapride Sulfabenzamide Ticlopidine Propiconazole Sulfadiazine Tiemonium Propionylpromazine Sulfadoxine Tilidine Propipocaine Sulfaethidole Propi verine Sulfaguanidine Tinidazole Sulfamethizole Propyphenazone Sulfamethoxazole Tocainide Prothiopendyl Sulfapyridine Sulfaquinoxaline Tolbutamide Sulindac Psilocin Sulpiride Tolnaftate Pyranocoumarin Sultiame Tolpropamine Pyribenzamine Sumatriptan Tolycaine Pyridoxine Suxibuzone Topotecan Pyrimethamine Tadalafil Torasemide Pyritinol Tramadol Pyrvinium Tamoxifen Tr anexa mic acid Telmisartan Quinapril Temaze pam Triadimenol Quinine Tenocixam Triallate Ramipril Terazosine Triamterene Ranitidine Terbinafine Triazolam Raubasine Trifluperazine Reboxetine Terbutryn Trifluperidol Remox ipride Terconazole Repaglinide Terfenadine Trimethobenzamide Tertalolol Trimethoprim Reserpine Tetracaine Riluzole Tetrazepam Tripelennamine

5 Triprolidine Valdecoxib Xipamide Tritoqualine Valsartan Tromantadine Vardenafil Tropisetron Venlafaxine Zolpidem Trospium Verapamil Zopiclone Trypta mine Vincamine Warfarin Xantinol

NEGATIVE MODE: Azelaic Acid Ethyl 2Amino 5nitrobenzophenone Aztreonam Ethyl sulfate D5 4Ben zamidosalicyclic acid Benserazid Furosemid 5(p Methylphenyl) phenylhydantoin Benzthiazide Hydrochlorothiazide 5Aminosalicylic acid Bezafibrate Morphin 3ßD glucuronide D3 6Mercaptourine Brallobarbital Pentobarbital Carbenoxolone Phenobarbital Acetylsalicylic Acid Chlorothiazide Propallylonal Adenine Cimetidine THC COOH Adenosine Cinchonine THC OH Adrenalone Cortisone Alprostadil Dinoprost Amobarbital Epine phrin e Amoxicillin Ethyl glucuronide Atorvastatin Ethyl glucuronide D5

6 FAST TOXICOLOGICAL SCREENING KIT – Code SC9000B

TECHNICAL FEATURES

Principle of the Method:

After optimizing the retention times of the analytes by means of the injection of the Mix Standard in LC MS/MS, it will be possible to inject the deproteinized plasma / blood to determine intoxication by unknown molecules.

Components of the kit (200 tests): All reagents are ready to use and stable 3 years at 2–8 °C.

Reagent A – Deproteinization Solution with internal Standard, 1 x 60 ml

Reagent E – Stabilizing Solution, 1 x 10 ml

Reagent F – Standard mix Solution, 1 x 1 ml

Reagent M1 – Mobile Phase M1, 8 x 500 ml

Reagent M2 – Mobile Phase M2, 8 x 500 ml

Minimum Instrumental equipment: ® Instrument API 3200 QTRAP Instrument API 4000 – 4500 QTRAP ® ® Instrument API 5500 QTRAP Operational Computer Optional equipment: Autosampler

Blood Collection Procedure: Tube with con anticoagulant EDTA Stable for about 4 weeks at 2 – 8 °C or 4 months at –20°C.

EUREKA srl – LAB DIVISION Head Quarter: VAT N° 01547310423 Via Enrico Fermi 25 E-mail:[email protected] 60033 Chiaravalle (AN) ITALY www.eurekaone.com Tel. +39 071 7450790 Fax + 39 071 7496579

This product meets all the requirements of Directive 98/79 / EC on in vitro diagnostic medical devices (IVD). The EC declaration of conformity is available on request.

Release N° 00 1 Fast To xicolog ical Screening Kit April 2019

7 PREANALYTICAL PHASE

PREPARATION OF STANDARD MIX FOR SYSTEM SET UP:

1) dilute 1 at 10 in Methanol the Reagent F – Standard mix Solution.

2) Prepare the following dilutions using the Reagent M1 – Mobile Phase M1. ® • For API 3200 QTRAP systems: add 100 ul of solution diluted at step 1 and add 900 L of Reagent M1 – Mobile Phase M1 • For API 4000 4500 QTRAP® systems: add 10 ul of solution diluted at step 1 and add 90 ul of Methanol and 900 L of Reagent M1 – Mobile Phase M1 ® • API 5500 QTRAP systems: add 2 ul of solution diluted at step 1 and add 98 ul of Methanol and 900 L of Reagent M1 – Mobile Phase M1

INJECTION :

• Inject 10 30 µl

This standard should be injected at the beginning of each session as a System Suitability Test to check the instrumental and chromatographic performance.

ANALYTICAL PROCEDURE

STEP 1 : In eppendorf dispense in sequence:

• 100 µl of SAMPLES + 300 µl of Reagent A – Deproteinization Solution with Internal Standard

STEP 2 : centrifuge at 10.000 rpm for 10 minutes

STEP 3 : dilute 100 µl of supernatant with 100 µl of Reagent E – Stabilizing Solution

INJECTION :

• Inject 30 µl

Release N° 00 1 Fast Toxicological Screening Kit April 2019

8

FAST TOXICOLOGICAL SCREENING KIT – Warnings

(THIS KIT HAS BEEN DEVELOP FOR SCIEX LC/MS SYSTEMS QTRAP CONFIGURATION)

INSTRUMENTAL CONDITIONS: Cromatographic Gradient

Gradient Profile Time (min) Flow Rate A (%) B (%) (µL/min) 0.00 500 90 10 10.00 1000 10 90 15.00 1000 10 90 15.50 500 90 10 18.00 500 90 10

Flow rate: 0.5 mL/min Column oven temperature: 40°C

Separation Column Drug Screen and Quant Analytical Column, 5 m 60 Å, PFP Propyl Column, 50 x 2.1 mm (P/N 9169552)

CODE ACCESSORIES DESCRIPTION PACKAGING Drug Screen and Quant Analytical Column, 5 m 60 SRE9169552 1 PK Å, PFP Propyl Column, 50 x 2.1 mm SK9001 PEEK Tubing O.D. × I.D. 1/16 in. × 0.005 in., red 1 PK PEEK Tubing O.D. × I.D. 1/16 in. × 0.010 in., blue Peek fitting Onepiece fingertight F130X S90199511 Prefilters Javelin 1 x 10 PK

9 FAST TOXICOLOGICAL SCREENING KIT (Reference Chromatograms/Spectra)

10 FAST TOXICOLOGICAL SCREENING KIT (Reference Chromatograms/Spectra)

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