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Somatic Embryogenesis and Regeneration of Endangered Cycad Species
Somatic Embryogenesis and Regeneration of Endangered Cycad Species R.E. Litz and P.A. Moon V.M. Chavez Avila Tropical Research and Education Center Jardin Botanico, Instituto de Biologia University of Florida Universidad Nacional Autonoma de Mexico 18905 SW 280 Street Apartado Postal 70-614 Homestead FL, 33031-3314 04510 Mexico DF USA Mexico Keywords: Somatic embryo, gymnosperm, Cycadales, conservation Abstract The Cycadales (Gymnospermae) include some of the world's most endangered and rare plant species. Many of the cycad species are known only as single specimen trees (e.g., Encephalartos woodii), as very small populations in the wild (e.g., Ceratozamia hildae) or have become extinct in the wild (e.g., Ceratozamia euryphyllidia). All cycads are dioecious, so that seed production is no longer possible with the rarest of the species. Conditions for induction of embryogenic cultures from leaves of mature phase trees of several species in the family Zamiaceae have been reported, and plants have been regenerated from somatic embryos. Embryogenic cultures of two species have been successfully cryopreserved. These strategies should contribute to the conservation of these endangered species and could lay the basis for commercial propagation of these beautiful but rare plants. INTRODUCTION The Cycadales represent the most ancient surviving group of higher plants, having arisen during the Permian era and flourished in the Mesozoic and Jurassic periods. They have been referred to as "living fossils" (Gilbert, 1984). Norstog (1987) considered that the cycads are unique for the study of the evolution of development in higher plants. There are only three extant cycad families, the Cycadaceae, Stangeriaceae and Zamiaceae, and these contain approximately 224 species. -
Systematic Studies on the Mexican Zamiaceae. I
SYSTEMATIC STUDIES ON THE MEXICAN ZAMIACEAE. I. CHROMOSOME NUMBERS AND KARYOTYPESI Instituto Nacional de Investigaciones Sobre Recursos Bi6ticos, Apdo. Postal 63, Xalapa, Veracruz, Mexico Chromosome numbers and karyotypes are reported for seven species in three genera, of Mexican cycads. Karyotypic analysis of Cycads is made difficult by differential contraction, of their large chromosomes but idiograms are presented. Satellite variation is reported for three species of Ceratozamia. No satellites are reported for the three species of Zamia. THECYTOTAXONOMILITC ERATUREon Mexican Francisco Javier Clavijero' (INIREB) at Xal- cycads has been rather sporadic. Sax and Beal apa. Watering the plants a day or two before (1934) reported 2n = 16for Ceratozamia mex- collection gave reasonably good results al- icana Brongn. and 2n = 18 for Dioon spinu- though cell divisions seemed to be variable. losum Dyer. They pointed out that chromo- Two different methods of pretreatment were some number and morphology may differ in tried in order to obtain adequate contraction different genera but the genomes of the species of the large cycad chromosomes. These con- within each genus seem to be similar. Marchant sisted of: 1) 1% aqueous solution of 1 ml ABN (1968) reported chromosome numbers of2n = (alpha-Bromonapthalene) dissolved in 100 ml 16 for C. mexicana, 2n = 18 for D. edule Dyer, of absolute ethanol (Dyer, 1979); 2) 0.05%, and 2n = 16 for Zamia fischeri Miq. He de- 0.1 %, and 0.2% aqueous solutions of colchi- scribed karyotypes and noted that the presence cine. The pretreatment in all cases was carried of telocentrics is apparently correlated with out for 5-6 hr in a refrigerator at ca. -
Comparative Biology of Cycad Pollen, Seed and Tissue - a Plant Conservation Perspective
Bot. Rev. (2018) 84:295–314 https://doi.org/10.1007/s12229-018-9203-z Comparative Biology of Cycad Pollen, Seed and Tissue - A Plant Conservation Perspective J. Nadarajan1,2 & E. E. Benson 3 & P. Xaba 4 & K. Harding3 & A. Lindstrom5 & J. Donaldson4 & C. E. Seal1 & D. Kamoga6 & E. M. G. Agoo7 & N. Li 8 & E. King9 & H. W. Pritchard1,10 1 Royal Botanic Gardens, Kew, Wakehurst Place, Ardingly, West Sussex RH17 6TN, UK; e-mail: [email protected] 2 The New Zealand Institute for Plant & Food Research Ltd, Private Bag 11600, Palmerston North 4442, New Zealand; e-mail [email protected] 3 Damar Research Scientists, Damar, Cuparmuir, Fife KY15 5RJ, UK; e-mail: [email protected]; [email protected] 4 South African National Biodiversity Institute, Kirstenbosch National Botanical Garden, Cape Town, Republic of South Africa; e-mail: [email protected]; [email protected] 5 Nong Nooch Tropical Botanical Garden, Chonburi 20250, Thailand; e-mail: [email protected] 6 Joint Ethnobotanical Research Advocacy, P.O.Box 27901, Kampala, Uganda; e-mail: [email protected] 7 De La Salle University, Manila, Philippines; e-mail: [email protected] 8 Fairy Lake Botanic Garden, Shenzhen, Guangdong, People’s Republic of China; e-mail: [email protected] 9 UNEP-World Conservation Monitoring Centre, Cambridge, UK; e-mail: [email protected] 10 Author for Correspondence; e-mail: [email protected] Published online: 5 July 2018 # The Author(s) 2018 Abstract Cycads are the most endangered of plant groups based on IUCN Red List assessments; all are in Appendix I or II of CITES, about 40% are within biodiversity ‘hotspots,’ and the call for action to improve their protection is long- standing. -
Ceratozamia Hildae Bamboo Cycad
Cycad Focus:Focus Focus Ceratozamia hildae Bamboo Cycad Photos by Bart Schutzman (unless otherwise noted) Cultivation of eral are different All the species I have Ceratozamia hildae worked with have an extended receptivi- ty period ranging from as early as Janu- by Tom Broome ary to as late as June, with everything in eratozamia hildae is my favorite between This makes it a little harder to C landscape cycad here in Florida It has pinpoint the exact time for each plant I an upright habit that is different from have to check all my plants every two or most other cycads, which have more of a three days during a four month period to fountain form It can be grown in a small- make sure I havent missed anything er spot so it makes a great accent plant Ceratozamia hildae is a very attrac- in a larger landscape, but can also be a tive cycad and is easy to grow This plant specimen plant in a smaller landscape should be on every new collectors list of The leaflets are grouped in unique cycads to try out It can get to be seven bowtie-shaped clusters Typically the feet tall and grows rapidly compared Immature species has green-emergent leaves, but a with many cycads, which is very reward- microstrobilus few rare brown-emergent individuals ing for anyone new to rare cycads Photo by MBC exist in collections (which I find very attractive) This species is easily grown in all of Florida It reacts well to fertilizer appli- cations I have had four flushes of leaves in one year on several individuals in my nursery They also mature fairly fast; males mature -
Mfnews Fall02-Web
Newsletter of Montgomery Botanical Center THE MONTGOMERY NEWS THE MONTGOMERY THE MONTGOMERY NEWS THE MONTGOMERY Values in Action Letter from the Executive Director Te rrence Walters, Ph.D. Most organizations are clearly in touch with their mission, their raison d’être. MBC’s purpose is to develop scientifically valuable collections of tropical plants, particularly palms and cycads, in a way that promotes con- servation, scientific investigation, educational opportunities, exemplary land- scape practices, and recognition of the “Montgomery” name. Ultimately, we aim to enhance the field of tropical botany through our activities. The practice of achieving one’s purpose requires working principles. At MBC, we are clear about our values, which include scientific excellence and integrity, as well as communication and teamwork. Teamwork applies not only to the staff, volunteers, directors, and members of MBC, but to our interactions with the botanical community at large. Two recent events help to illustrate this point. In 2001, MBC was the initiator and facilitator of an expedition to collect the endangered cycads of Mexico. With the objective of creating scientifically valuable ex situ collections that would relieve pressure on native populations, we collabo- rated with two other cycad botanical gardens with similar goals—Xalapa Botanical Garden in Veracruz, Mexico and California’s Lotusland. Botanists from each institu- tion worked as a team during the two-week collecting trip. Duplicate collections for the three organizations were obtained to allow easy access to these rare cycads by researchers and students in both countries, and to ensure the future of these species. One of our more recent attempts at a synergistic endeavor was the international workshop held at MBC on Cycad Classification Concepts on April 7–9. -
Zamiaceae, Cycadales) from the European Oligocene and Lower Miocene
Acta Palaeobotanica 54(2): 231–247, 2014 DOI: 10.2478/acpa-2014-0012 New fossil records of Ceratozamia (Zamiaceae, Cycadales) from the European Oligocene and lower Miocene ZLATKO KVAČEK Charles University in Prague, Faculty of Science, Institute of Geology and Palaeontology, Albertov 6, CZ 12843 Praha 2, Czech Republic; e-mail: [email protected] Received 15 July 2014; accepted for publication 12 November 2014 ABSTRACT. New compression leaf material of Ceratozamia (Zamiaceae) has been recognised in the European Cenozoic. A leaflet of Ceratozamia floersheimensis (Engelhardt) Kvaček was recovered among unidentified mate- rial from the Oligocene of Trbovlje, former Trifail, Slovenia, housed in old collections of the Austrian Geological Survey, Vienna. It is similar in morphology and epidermal anatomy to other specimens previously studied from the lower Oligocene of Flörsheim, Germany and Budapest, Hungary. A fragmentary leaflet assigned to C. hof- mannii Ettingsh. was recovered in the uppermost part of the Most Formation (Most Basin in North Bohemia, Czech Republic) and dated by magnetostratigraphy and cyclostratigraphy to CHRON C5Cn.3n, that is, the latest early Miocene. It yielded excellently preserved epidermal structures, permitting confirmation of the generic affin- ity and a more precise comparison with this lower Miocene species previously known from Austria (Münzenberg, Leoben Basin) and re-investigated earlier. Both the Oligocene and Miocene populations of Ceratozamia are based on isolated disarticulated leaflets matching some living representatives in the size and slender form of the leaf- lets. Such ceratozamias thrive today in extratropical areas near the present limits of distribution of the genus along the Sierra Madre Orientale in north-eastern Mexico, in particular C. -
Cycads in the South 'Florida Landscape'
Cycads in the South ‘Florida Landscape’ JODY L. HAYNES Introduction that receive no more than a couple of inches of rain per year. Cycads are ancient, palm-like, evergreen gymnosperms (cone-bearing plants) of the Dioon edule is probably the most cold-hardy of Division Cycadophyta. Represented by three all the cycads. In the 1989 freeze, parts of families—Cycadaceae, Stangeriaceae, and Zami- Lakeland, FL, got down to 17°F. Most king aceae—the cycads are composed of approxi- sagos were completely defoliated, while D. edule mately 200 species in 11 genera—Bowenia, plants only experienced tip burn. Ceratozamia, Chigua, Cycas, Dioon, Encepha- lartos, Lepidozamia, Macrozamia, Microcycas, Many cycads are also salt tolerant. For example, Stangeria, and Zamia. in a particular habitat in Mexico, Dioon plants hang over a cliff and are constantly assaulted Although many cycads superficially resemble with salt spray from the Gulf of Mexico. palms, these two groups of plants are in no way related. In fact, cycads are more closely related With our sand- and limestone-based soils here in to pine trees than to palms. During the age of the south Florida, it can be difficult to grow some dinosaurs cycads were the most abundant plants types of plants. However, the majority of cycads on Earth, whereas palms did not show up on thrive here. As a result, cycads make perfect, Earth for another 150 million years. easy to maintain plants for our landscapes. In fact, one cycad species is native to Florida. The Cycads are dioecious plants, which means that common name for the plant is "coontie", which there are separate male and female plants. -
O Attribution — You Must Give Appropriate Credit, Provide a Link to the License, and Indicate If Changes Were Made
COPYRIGHT AND CITATION CONSIDERATIONS FOR THIS THESIS/ DISSERTATION o Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. o NonCommercial — You may not use the material for commercial purposes. o ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original. How to cite this thesis Surname, Initial(s). (2012) Title of the thesis or dissertation. PhD. (Chemistry)/ M.Sc. (Physics)/ M.A. (Philosophy)/M.Com. (Finance) etc. [Unpublished]: University of Johannesburg. Retrieved from: https://ujcontent.uj.ac.za/vital/access/manager/Index?site_name=Research%20Output (Accessed: Date). AN INTEGRATIVE APPROACH TOWARDS SETTING CONSERVATION PRIORITY FOR CYCAD SPECIES AT A GLOBAL SCALE BY RESPINAH TAFIREI Minor dissertation submitted in partial fulfilment of the requirements for the degree of MASTER OF SCIENCE IN ENVIRONMENTAL MANAGEMENT Faculty of Science UNIVERSITY OF JOHANNESBURG August 2016 SUPERVISOR Dr K. Yessoufou CO-SUPERVISOR Dr I.T. Rampedi DEDICATION This work is dedicated to my parents. iii ACKNOWLEDGEMENTS My entire family, mainly my beautiful children; Thabo, Ryan, Chloe, my nephew, Tinashe, as well as my husband: You were there for me throughout this journey. I am deeply appreciative and grateful for the support and rapport I received from my dear husband, Simon. Your patience did not go unnoticed. Thank you from the bottom of my heart. I am also very grateful for the support and scientific guidance I received from Dr. -
Threatened and Rare Ornamental Plants
Journal of Agriculture and Rural Development in the Tropics and Subtropics Volume 108, No. 1, 2007, pages 19–39 Threatened and Rare Ornamental Plants K. Khoshbakht ∗1 and K. Hammer 2 Abstract The application of IUCN criteria and Red List Categories was done for ornamental plants. Main sources of the study were Glen’s book, Cultivated Plants of Southern Africa (Glen, 2002) and the Red List of Threatened Plants, IUCN (2001). About 500 threatened ornamental plants could be found and presented in respective lists. Rare ornamental plants with 209 species is the largest group followed by Vulnerable (147), Endangered (92), Indeterminate (37), Extinct (6) and finally Extinct/Endangered groups with 2 species. A weak positive correlation (r = +0.36 ) was found between the number of threatened species and the number of threatened ornamental species within the families. Keywords: ornamental plants, IUCN criteria, red list 1 Introduction Whereas red lists of threatened plants are being highly developed for wild plants and even replaced by green lists (Imboden, 1989) and blue lists (Gigon et al., 2000), ornamental plants still lack similar lists. A statistical summary of threatened crop plant species was published by Hammer (1999) showing that roughly 1000 species of cultivated plants (excluding ornamentals) are threatened (see also Lucas and Synge (1996). An attempt was recently made towards a red list for crop plant species, which presents about 200 threatened cultivated (excluding ornamentals) plants in the IUCN categories (Hammer and Khoshbakht, 2005b). Now an effort is made to include ornamentals. IUCN has defined six categories for threatened plants – Extinct, Extinct/Endangered, Endangered, Vulnerable, Rare and Indeterminate (see IUCN (2001) for definitions). -
Jody L. Haynes
JODY L. HAYNES CONTACT INFORMATION Professional Scientific Signature Trees & Palms, Inc. IUCN/SSC Cycad Specialist Group 14290 SW 232 St., Miami, FL 33170 P.O. Box 971063, Miami, FL 33197-1063 [email protected] [email protected] EMPLOYMENT HISTORY Business Development & Estimating Manager, Signature Trees & Palms, Miami, FL (2010-present) Estimating Manager, Arazoza Bros. Landscaping & Irrigation, Homestead, FL (2008-2009) Purchasing Manager, Arazoza Bros. Landscaping & Irrigation, Homestead, FL (2007-2008) Procurement Manager, Tropics North Landscaping, Homestead, FL (2006) Cycad Biologist, Montgomery Botanical Center (MBC), Coral Gables, FL (2003-2006) Tri-county Program Extension Agent, Florida Yards & Neighborhoods (FYN) Program, University of Florida/Miami-Dade County Extension, Homestead, FL (2000-2002) Adjunct Lecture, Laboratory, and Independent Study Instructor, Miami-Dade College, Kendall & Homestead, FL, campuses (1997-2000); Courses taught: General Biology Lecture, General Biology Lab, Environmental Science Lecture, Independent Study Research Biologist, University of Florida Tropical Research & Education Center, Homestead, FL (1997-1999) Graduate Teaching Assistant and Independent Research Coordinator for Miami Science Magnet high school students, Florida International University, Miami, FL (1994-1996) Aquatic Biologist, U.S. Army Corps. of Engineers, New Orleans, LA (1990-1992) Tour Guide, Voyageur Canoe Swamp Tours, Slidell, LA (1990-1992) Temporary Display Coordinator, University of Nebraska State Museum, Lincoln, NE (1986-1989) EDUCATION Ph.D. program, 1994-1996, Biological Sciences, Florida International University; primary interest: molecular genetics and phylogenetic systematics (69 cr. hr.; GPA 3.786) Ph.D. program, 1992-1994, Biological Sciences, Mississippi State Univ.; transf. to FIU (31 cr. hr.; GPA 3.677) Master of Science, 1992, Biological Sciences, University of New Orleans (52 cr. -
Morphometric and Geographical Variation in the Ceratozamia Mexicana Brongn
Biological Journal of the Linnean Society, 2016, 119, 213–233. With 8 figures. Morphometric and geographical variation in the Ceratozamia mexicana Brongn. (Zamiaceae) complex: evolutionary and taxonomic implications ANWAR MEDINA-VILLARREAL and JORGE GONZALEZ-ASTORGA* Laboratorio de Genetica de Poblaciones, Red de Biologıa Evolutiva, Instituto de Ecologıa, A. C., km 2.5 Antigua Carretera a Coatepec No. 351, Xalapa, 91070, Veracruz, Mexico Received 9 November 2015; revised 31 January 2016; accepted for publication 1 February 2016 The Cycadales contain 11 nonproblematic genera and approximately 331 species where, in a number of cases, delimitation and circumscription is not so clear. Ceratozamia, the most diverse cycad genus in Mexico, clearly illustrates this situation because a large percentage (close to 70%) of the 28 currently valid species in the genus was recently described on the basis of inconspicuous differences. We evaluated population-level morphological variation (i.e. phenotype) in related plants of Ceratozamia that share geological and ecological units, seeking a refined taxonomic assessment of their populations in an explicitly evolutionary context. The populations/species of interest included the type species Ceratozamia mexicana, as well as the taxa Ceratozamia brevifrons, Ceratozamia decumbens, and Ceratozamia morettii. Data analysis was performed using uni- and multivariate techniques on morphological variability, which showed statistically significant differences. The characters ‘leaflet width’ and ‘leaflet shape’ supported diagnostic distinctions between groups of populations, and implied a classificatory rearrangement with nomenclatural consequences. This research is an initial step in the detailed, statistics-oriented study of phenotypic variation in the Ceratozamia populations of central Veracruz in Mexico that will allow the construction of evolutionary hypotheses in the context of phylogeography and historical demography. -
A Review of Research on the Cycad Genus Ceratozamia Brongn
53 (2) • May 2004: 291–297 Vovides & al. • Research on Ceratozamia A review of research on the cycad genus Ceratozamia Brongn. (Zamiaceae) in Mexico Andrew P. Vovides1, Dolores González1, Miguel Angel Pérez-Farrera2, Sergio Avendaño1, Cristina Bárcenas1 1 Departamento de Sistemática Vegetal, Instituto de Ecología, A.C., Apdo Postal 63, Xalapa, Veracruz, 91000 Mexico. [email protected] (author for correspondence); [email protected]; avenda@ ecologia.edu.mx; [email protected] 2 Escuela de Biología, Universidad de Ciencias y Artes del Estado de Chiapas, Calz. Samuel León Brindis 151, Tuxtla Gutierrez, Chiapas, 29000 Mexico. [email protected] The genus Ceratozamia contains more than 20 species distributed in eastern and southern Mexico, with exten- sions into Central America. Morphology, anatomy, and molecular (ITS) data allow resolution of some aspects of species relationships, provide correlations with ecology, and suggest phytogeographic hypotheses. Some species complexes, such as those centered around C. norstogii and C. miqueliana, are restricted to southern- west Mexico, in what are regarded as Pleistocene floristic refugia. The low level of divergence of ITS sequences among taxa to the north of the Trans-Mexican Neovolcanic mountain range suggests a pattern of recent, and perhaps rapid, speciation. KEYWORDS: Cycadales, Middle America, phytogeography, refugia, speciation. secondary products, certain toxins are peculiar only to INTRODUCTION cycads, such as cycasin. Macrozamin and the neurotoxin After a period of relative stagnation since the pio- 2-amino-3-(methylamino)-propanoic acid (BMAA) are neering work of Charles Joseph Chamberlain in the early considered synapomorphies of the cycad clade of the 20th century (Norstog & Nichols, 1997), renewed inter- seed plants (Daly & al., 2001).