Museum Technical Report No. 137

Conservation of the Cottogaster (Percina copelandi sp. clade): Applying molecular phylogenetics and phylogeograpy towards the reconstruction of evolutionary history (YEAR 2)

Mark A. Dugo1, William T. Slack1 and Brian R. Kreiser2

1Mississippi Museum of Natural Science 2148 Riverside Drive Jackson, Mississippi 39202-1353

2Department of Biological Sciences The University of Southern Mississippi 118 College Drive #5018 Hattiesburg, MS 39406

Funded by U.S. Fish and Wildlife Service U. S. Fish and Wildlife Service Section 6, Project No. 491, Segment 22

Final Report submitted to Mississippi Department of Wildlife, Fisheries, and Parks Museum of Natural Science 2148 Riverside Drive Jackson, MS 39202-1353

January 2008

THIS PROJECT WAS FUNDED BY THE U.S. FISH AND WILDLIFE SERVICE THROUGH A SECTION 6 COOPERATIVE AGREEMENT WITH THE MISSISSIPPI DEPARTMENT OF WILDLIFE, FISHERIES, AND PARKS.

THIS FINAL REPORT HAS BEEN PREPARED AND SUBMITTED TO THE U.S. FISH AND WILDLIFE SERVICE TO FULFILL FEDERAL-AID REQUIREMENTS OF THE MISSISSIPPI ENDANGERED SPECIES PROJECT E-1, SEGMENT 21.

MISSISSIPPI MUSEUM OF NATURAL SCIENCE TECHNICAL REPORTS ARE NOT PEER- REVIEWED. THIS DOCUMENT SHOULD BE CITED AS AN UNPUBLISHED REPORT TO THE U.S. FISH AND WILDLIFE SERVICE.

2

Problem and Need

The subgenus Cottogaster (Etheostomatini, , Percina), recently classified as the “copelandi” species clade (Near 2002) includes three species, the channel darter

(Percina copelandi), the pearl darter (Percina aurora) and the coal darter (Percina brevicauda) (Suttkus et al. 1994). Percina copelandi is widely distributed in the

Mississippi Basin with disjunct populations occurring in the Great Lakes region of the

U.S. and Canada, the Ohio River, the upper Cumberland and Tennessee Rivers, and the

Red, Ouachita and Arkansas Rivers west of the Mississippi Valley. Percina aurora is described from the Pearl and Pascagoula River drainages (Louisiana, Mississippi) of the

Gulf coastal plain, and P. brevicauda is described from the Black Warrior, Cahaba and

Coosa Rivers (Alabama, AL) of the Mobile Basin (Suttkus et al. 1994).

The Cottogaster is an imperiled group of fishes experiencing extirpation and population declines throughout their range (Goldchild 1993; Suttkus et al. 1994; Warren et al. 2000). Currently, no federal protection exists for Cottogaster, although many populations are listed as threatened or endangered by state agencies, and all three species are recognized by the AFS as deserving conservation merit (P. copelandi, concern species; P. aurora, endangered; P. brevicauda, threatened ) (Warren et al. 2000).

Percina copelandi maintains viable and in some cases abundant populations in the

Ouachita Highlands of Arkansas and Oklahoma where it is listed as “apparently secure”

(TNC Heritage Rank = S4) (NatureServe 2003). However, population declines have been observed in the upper Tennessee River, northeastern reaches of the Ohio River system and tributaries of the Great Lakes. Percina copelandi is listed as “imperiled” (TNC

Heritage Rank = S2) in Virginia, West Virginia, Tennessee, Indiana, Ohio and New

3 York, and is listed as “critically imperiled” (TNC Heritage Rank = S1) in Vermont,

Pennsylvania and Michigan (NatureServe 2003). Percina aurora is listed as S1 in

Mississippi and Louisiana, and was recognized as a candidate species in 1999 (D.

Drennen, USFWS, pers. comm.). Percina aurora was last taken from the Pearl River

drainage in the early 1970’s and is assumed extirpated (Ross 2001). Recent efforts documented the persistence of P. aurora in the Pascagoula River drainage; however, their

occurrence is uncommon (Slack et al. 2002; Slack et al. 2005). Percina brevicauda,

listed as S2 in Alabama maintains its largest populations in the Cahaba River. A 2001

survey expanded the known range of the coal darter in the Black Warrior system,

although abundance was notably reduced at sites where they were collected in 1998

(Shepard et al. 2002). Percina brevicauda are reported as rare in the Coosa River

(Boschung and Mayden 2004; Carol Johnson, Auburn University, pers. comm.). It has

been reported that some population declines of P. brevicauda are correlated with

impoundments within the Black Warrior and Coosa River Systems (Suttkus et al. 1994).

The Cottogaster are a monophyletic clade of Percina (Near 2002) and represent a

unique component of biodiversity occurring in a region of high diversity and endemism

globally recognized in species richness, as well as for its need of conservation efforts

(Williams and Miller 1990; Moyle and Leidy 1992; Warren et al. 2000). The disjunct

distributions common within the clade increases their likelihood of population declines

and potentially even the extinction of species (Angermeier 1995; Abrams 2002). A

conservation assessment is needed for the Cottogaster, framed in the context of its shared

phylogentic history. Our current understanding of ecology and life history for

Cottogaster is limited in part due to a paucicity of life history data and a lack of

4 phylogenetic resolution among intraspecific populations and between each of the species within the clade. Reported account of life history for the Cottogaster are usually drawn from observations not associated with dedicated ecological studies (see Suttkus et al.

1994; pp. 12-13, 17, 19) and our current understanding of life history and habitat use remains a patchwork of accounts with the extrapolation of data from one population to another. This has resulted in numerous inconsistencies when attempting to construct a scientifically supported view of the life history strategies exhibited by this unique clade of fishes (Slack et al 2002; Slack et al. 2005). Additionally, a lack of phylogenic

resolution for the Cottogaster impedes biologically relevant delineation of evolutionary significant units (Moritz 1994) and potential surrogate populations for conservation related reintroduction programs (Schofield and Ross 2003).

Research Objectives

The primary objective of this study is to reconstruct the evolutionary history

within Cottogaster. This research approach remains paramount considering 1) the

imperilment of the group as a whole, and the need for conservation efforts for these taxa;

2) a philosophical appreciation of the concept that patterns of life history variation often carry a historical signal (Brooks et al. 1992; McLennan 1994, Turner et al. 1996); and 3)

the management and recovery of the Cottogaster requires knowledge of their life history.

In theory, the success of scientifically supported management practices should be

positively correlated with resolution of taxonomic diversity, phylogeographic history and

ecological character evolution.

Near (2002) indicated that Cottogaster is a monophyletic clade of Percina, and sister to Imostoma but determining the inter- and intraspecific relationships was beyond

5 the scope of his analysis. In the original description of P. aurora and P. brevicauda,

significant morphometric and meristic variation was noted between northeastern and southwestern populations of P. copelandi (Suttkus et al. 1994). For some characters both

P. aurora and P. brevicauda were most similar to populations of the Great Lakes, while for other characters they were found more similar to populations in the Ouachita

Highlands. In some cases P. copelandi was intermediate between P. aurora and P. brevicauda. In fact, meristic and morphometric variation observed in P. copelandi, along with variation of electromorph mobility (Page and Whitt 1973), suggest that P. copelandi may be polytypic.

We will address phylogenetic relationships within Cottogaster with focus on the

following questions:

Phylogeography of Percina copelandi

Question 1) What are the relationships between populations of Percina copelandi east

(upper Tennessee, Ohio, Great Lakes) and west (Ouachita Highlands) of the Mississippi

River?

Question 2) What is the origin of Cottogaster on the Gulf coastal plain and the Mobile

Basin?

Expected Results and Benefits

Results of this analysis are three-fold and include, 1) work towards the delineation

of evolutionary significant units (Moritz 1994) minimally identified through reciprocally

monophyletic mtDNA haplotypes, thereby assisting in the identification of populations

6 warranting protection under the Endangered Species Act (Waples 1995); 2) establishing a historical framework for resolving ecological character evolution when overlain with data of life history variation and ecology (Brooks et al. 1992; McLennan 1994; Turner et al.

1996); 3) identifying evolutionarily and ecologically relevant surrogate populations for

laboratory based studies (Schofield and Ross 2003); and potential re-introduction programs (2003 Pearl darter workshop, MMNS, Jackson MS; Pat Rakes, Conservation

Fisheries Inc., pers. comm.)

Study approach

Collections - The acquisition of Cottogaster tissue for DNA analysis was conducted

through field collection by PI’s, gifted material to MMNS from various regional field

biologists and through other molecular laboratories that maintain catalogs of collected

DNA. DNA sequences were also downloaded from GenBank when available. The

MMNS protocol for field collecting DNA from fishes included when feasible, the

collection of a voucher series of specimens fixed in formalin in addition to the series of

95-100% ETOH preserved specimens for DNA material. Specimens intended as vouchers

were euthanized in MS-222 (tricaine methanosulfonate) or clove oil prior to fixation. In

cases where too few specimens were available (generally less than 5 per site), the field

protocol was modified such that fin clips for DNA tissue were taken, stored in 95%-

100% ETOH or SED buffer and the remainder of the specimen fixed formalin as a

voucher. All fishes collected by PI’s were deposited in the Mississippi Museum of

Natural Science Ichthyology Collection.

7 Molecular Methodology –

Total genomic DNA was extracted from fin clips tissue using the Qiagen Dneasy

extraction kit and quantified through spectrophotometry using a NanoDrop ND-1000

(Thermo-Fisher Scientific. Waltham, Massachusetts). The polymerase chain reaction

(PCR) was used to amplify cytochrome b of the mitochondrial genome using the L15269

and H16462 from Song et al. (1998). All PCR amplifications were conducted in a total

volume of 25 !l using 50 mM KCl, 10 mM Tris-HCl (pH 8.3), 0.01% gelatin, 2 mM

MgCl2, 200 !M dNTPs, 1.0 unit Taq polymerase, 0.3 !M of each primer, approximately

100 ng template DNA and water to the final volume. PCR cycling conditions consisted of an initial 1 min denaturing step at 95"C followed by 30 cycles of 1 min at 95"C, 1 min at 50"C and 3 min at 72"C. A final elongation step of 7 min at 72"C completed the cycle.

These PCR products were then Exo-Sap cleaned (USB Corp. Cleveland, Ohio) and used

as a template in a cycle sequencing reaction using the ABI BigDyeTM Terminator cycle sequencing kit (Foster City, CA). The actual gel runs were performed at the Iowa State

University DNA Sequencing and Synthesis Facility. Sequences were edited and aligned using Sequencher v. 4.1 (GeneCodes Co., Madison, WI).

All phylogenetic analyses were conducted with PAUP* 4.0b10 (Swofford 2002)

using neighbor-joining (NJ) (Saitou and Nei 1987) criteria performed on Kimura two- parameter distances (Kimura 1980) and maximum parsimony (MP). For both NJ and

MP, a heuristic search was performed using tree bisection-reconnection (TBR) branch

and bound tree search algorithm, with simple addition using 5000 maximum trees, 50

replicates per round and 5 tree held during step-wise addition. Bootstrapping with 1,000

8 rounds of resampling was performed to assess relative support for tree nodes.

Phylogenetic trees where viewed using TREEVIEW 1.40 (Page 1996).

Microsatellite Screening – Published mirosatellite markers designed for species closely

related to Cottogaster (Tonnis 2006, Beneteau et al. 2007, Khudamrongsawat et al. 2007,

Li et al. 2007) were preliminarily screened for variability and optimization using a

LICOR 4300 DNA Sequencer (LICOR Co. Lincoln, Nebraska).

Results and Discussion

Extracted DNA was obtained for a total of 147 specimens of Cottogaster (Percina aurora n=24, Appendix A; Percina brevicauda n=27, Appendix B.; Percina copelandi n=96, Appendix C.)

Cytochrome b sequences for Cottogaster were obtained for a total of 27 individuals from 14 localities including two locations for P. aurora, 2 P. brevicauda sites and 10 P. copelandi sites: 5 west of the Mississippi River and 5 east of the Mississippi

River (Table 1; Figure 1).

PCR amplifications generated at MMNS successfully spanned across the entire

cyt b gene (1140 bp). Three segments of the sequence strand were trimmed to make for

an analyzed sequence strand of 1057 base pairs with no missing data. A total of 827

characters were recorded as constant, 111 variable characters as parsimony uninformative

and 119 parsimony informative characters were identified.

Pairwise genetic divergences are presented in Table 2. The interspecific

divergence of the Cottogaster, according to their current taxonomic status, ranges from

0.0154 (P. aurora and P. brevicauda) to 0.0557 (P. aurora and P. copelandi from

Verdigris River). These values are within the range observed among closely related

9 percid species, however the sequence divergence for P. aurora and P. brevicauda is

moderately low relative to reports for cytochrome b in other sister darter species (Near et

al. 2001; George et al. 2006). Intraspecific divergence values for P. copelandi revealed a

high level of polymorphism within this species ranging from 0.0014 (Bird Creek, OK and

Verdigris, KS; Little River/Glover and Little River/Mountain Fork) to 0.0535 (Green

River and Little River/Mountain Fork).

Monophyletic clades recovered at the species level were essentially identical for both NJ and MP (only one four most parsimonious MP trees is presented; Figure 2).

Consistent with previous reports of morphology (Suttkus et al. 1994) and molecular

analysis (Near 2002), Percina aurora and P. brevicauda were recovered as sister species

with moderate to strong bootstrap support for the monophyly of each species (Percina

aurora 94% for MP and 95% for NJ; Percina brevicauda 72% for MP and 71% for NJ).

Percina copelandi was identified as polytypic in all analyses with two distinct clades

recovered for populations sampled. The first clade consisted of all populations and

individuals assayed east of the Mississippi River (i.e. Lake Champlain, Ohio River

drainage) plus northern populations assayed west of the Mississippi River (i.e. Arkansas

River drainage) (100% bootstrap support for MP and 100% bootstrap support for NJ).

The second major clade represented the two most southern drainages sampled west of the

Mississippi River, the Ouachita and Red river drainages (90% bootstrap support for MP

and 91% bootstrap support for NJ).

Resolution of the interspecific relationships within the Cottogaster remains inconclusive at this time and will require the analysis of more individuals, locations and gene regions. In the MP analysis, the sister taxa P. aurora and P. brevicauda were

10 monophyletic to the rest of the Cottogaster analyzed (96% bootstrap support). The NJ analysis recovered P.copelandi from the Arkansas and Red River drainages along with the sister taxa P. aurora and P. brevicauda as monophyletic with respect to the remaining

P. copelandi samples (58% bootstrap support). Data presented herein represents our preliminary findings working towards phylogenetic resolution of the Cottogaster. We stress that these data are a first phase preliminary analysis; however, we can confidently validate the species level recognition of P. aurora and P. brevicauda as sister species and identify polymorphism within P. copelandi divergent enough to recognize at least two or more species. We do not have enough data to address major biogeographic questions for this clade of fishes. Nevertheless, it is clear that in reference to question one, variation for cytochrome b in P. copelandi populations east of the Mississippi River is limited and appears most similar to northern populations of P. copelandi west of the Mississippi

River (i.e. Arkansas River drainage minimally). Regarding the affinity of P. aurora and

P. brevicauda on the Gulf coastal Plain (question 2) our data suggest a potential affinity with Gulf coastal plain populations of P. copelandi from the Red and Ouachita River drainages. Preliminary conclusions on the biogeogprahy of the Cottogaster are that the evolutionary history of the clade is likely the result of both vicariant speciation and dispersal. Depth of conclusions and more significant contributions towards meaningful conservation planning awaits additional data, including an increase of sampling localities, more individuals per locality and the inclusion of additional molecular markers (i.e nuclear S7 and microsatellites).

11 Table 1. Collection data for material represented in current molecular analysis.

Species n Locality Genbank Acc DNA Cat. Voucher Cat. Latitude Longitude # # # dd dd

APercina aurora 2 Chickasawhay River, Stonewall, Clark Co., MS MMNS 190 32.1110 -88.8086 BPercina aurora 1 Leaf River, Eastabuchie, Jones Co., MS AF386566 TJN 149 UAIC 10458.14 31.4400 -89.3007 CPercina brevicauda 1 Black Warrior, Locust Fork, Jefferson Co., AL MMNS 417 33.8089 -86.8010 DPercina brevicauda 1 Cahaba River, Pratts Ferry, Bibb Co., AL AF386567 TJN 148 UAIC 11679.01 33.0189 -87.0789 EPercina copelandi 2 Green River, Munfordville, Hart Co., KY MMNS 281 37.2692 -85.8840 FPercina copelandi 3 Little Kanawha, Copper Run, Gilmer Co., WV MMNS 167 38.9799 -80.7176 GPercina copelandi 2 Lake Champlain, Winooski River, Chittendon Co., MMNS 821 44.4414 -73.2195 VT HPercina copelandi 1 Caddo River, Amity, Clark Co., AR MMNS 145 34.2895 -93.4509 IPercina copelandi 3 Little River/Glover, North Pole, McCurtain Co., OK MMNS 667 MMNS 47905 34.0976 -94.9023 JPercina copelandi 2 Verdigris, Bird Creek, Osage Co., OK MMNS 728 MMNS 47512 36.4828 -96.0363 KPercina copelandi 3 Verdigris, Sycamore, Montgomery Co. KS MMNS 700 MMNS 47858 37.3269 -95.6839 LPercina copelandi 4 Little River/ Mountain Fork, Hatfield, Polk Co., AR MMNS 282 NCSM 37383 34.5053 -94.4309 MPercina copelandi 1 Green River, Greensburg, Green Co., KY AF386568 TJN 89 37.2541 -85.5028 *Percina copelandi 1 Green River, Green Co., KY AY374283 - -

OUTGROUPS

Percina lenticula 1 Leaf River, Jones Co., MS AF386585 TJN 211 Percina pantherina 1 Big Eagle Creek, Red River, OK AF386558 TJN 325 Percina vigil 1 Bayou de Chien, Hickman Co., KY AF386569 TJN 90

Superscript letters adjacent to species name refers to points on the distribution map (Figure 1). * Coordinate data for the specimen was unavailable. see; Sloss et al. 2004 Figure 1. Geographic distribution for the Cottogaster with collection sites for molecular analysis indicated by solid dots. Maroon area = P. aurora; Yellow area = P. brevicauda;

Tan areas = P. copelandi

13

Table 2. Pairwise sequence divergence for 1057 base pairs of cytochrome b among species and major drainage sampled for this study.

Percina Percina Little River - Little River - Caddo River, Bird Creek, OK Verdigris, KS Green, KY Little aurora brevicauda Glover, Ok Mt. Fork, AR AR Kanawha, WV

Red R. Red R. Ouachita R. Arkansas R. Arkansas R. Ohio R. Ohio R.

drainage drainage drainage drainage drainage drainage drainage

Percina copelandi

Percina aurora - Percina brevicauda 0.0154 - Gulf Coast comparisons

Little River - Glover Percina 0.0418 0.0412 - Little River - Mt. Fork 0.0430 0.0412 0.0140 - Caddo River, AR 0.0438 0.0426 0.0327 0.0322 -

Bird Creek, OK cop 0.0532 0.0534 0.0481 0.0513 0.0492 - Verdigris, KS 0.0557 0.0549 0.0493 0.0526 0.0501 0.0014 - e Green, KY landi 0.0508 0.0508 0.0523 0.0535 0.0525 0.0067 0.0075 - Little Kanawha, WV 0.0539 0.0543 0.0485 0.0524 0.0492 0.0039 0.0045 0.0072 - Lake Champlain, VT 0.0545 0.0540 0.0478 0.0518 0.0492 0.0033 0.0034 0.0056 0.003

14

P. lenticula P. vigil P. pantherina P. brevicauda Cahaba R.

98 P. brevicauda Black Warrior R. P. aurora Chickasawhay R. P. aurora Leaf R. P. aurora Chickasawhay R. P. copelandi – Caddo R. P. copelandi – Little R./Glover 96 90 P. copelandi – Little R./Glover 100 P. copelandi – Little R./Glover P. copelandi – Little R./Mt. Fork P. copelandi – Little R./Mt. Fork P. copelandi – Little R./Mt. Fork 68 P. copelandi – Little R./Mt. Fork P. copelandi – Bird Crk. P. copelandi – Bird Crk. P. copelandi – Verdigris R. P. copelandi – Verdigris R. 100 P. copelandi – Little Kanawha R. P. copelandi – Little Kanawha R. P. copelandi – Green R. P. copelandi – Green R. P. copelandi – Green R. P. copelandi – Lake Champlain R. P. copelandi – Lake Champlain R. P. copelandi – Little Kanawha R. P. copelandi – Green R. P. copelandi – Verdigris R.

Figure 2. Phylogeny recovered in maximum parsimony (one of the four most parsimonious trees). Bootstrap values (1000 pseudoreplicates) indicated for major nodes within Cottogaster. Outgroups include Percina lenticula, P. pantherina, and P. vigil.

15 Microsatellite Screening – A total of 13 microsatellite loci from four published papers were screened across 30 reaction periods for a total of 436 individual reactions (Tonnis

2006: Eca11EPA, Eca 4811EPA, ECA49EPA; Beneteau et al. 2007: Ebl3, Ebl4;

Khudamrongsawat et al. 2007: Eche005; Li et al. 2007: YP13, YP30, YP62, YP73, YP79,

YP106, YP108). Preliminary screening of these loci produced three loci with visible bands (YP30, YP73 and YP106; Li et al. 2007). Both YP30 and YP73 showed no variability across 36 individual Cottogaster samples representing each of the three species. YP106 showed variability with 10 observable alleles across 125 samples.

Preliminary effort with microsatellite amplification of Cottogaster DNA

positively demonstrates for the group, cross species amplification using primers designed

for closely related percid species. Additional optimization is needed for loci YP106 using the LICOR 4300 and additional loci should be screened.

16 Acknowledgements

DNA samples were provided by Mark Ferguson (Vermont Fish and Wildlife

Department), Dr. Carol Johnston (Auburn University), Dr. Bernie Kuhajda (University of

Alabama - Tuscaloosa), Dr. Andrew Simon (University of Minnesota), Tim Stecko

(University of Pennsylvania), Matt Thomas (Kentucky Department of Fish and Wildlife

Resources) and Dr. Stuart Welsh (University of West Virginia). Assistance with molecular work at the Mississippi Museum of Natural Science was provided by Meredith

Montgomery. We also thank Libby Hartfield and Charles Knight for their support at

MMNS.

17 Literature Cited

Abrams, P.A. 2002. Will small population sizes warn us of impeding extinctions?

American Naturalist 160: 293-305.

Angermeier, P.L. 1995. Ecological attributres of extinction-prone species loss of

freshwater fishes in Virginia. Conservation Biology 9: 143-158.

Beneteau, C.L., N. E. Mandrak, D.D. Heath. 2007. Characterization of eight

polymorphic microsatellite DNA markers for the greenside darter,

blennioides (Percidae). Molecular Ecology Notes 7:641-643.

Boschung, H.T., and R.L. Mayden. 2004. Fishes of Alabama. Smithsonian Institute.

Washington D.C. 736 pp.

Brooks, D.R., R.L. Mayden, and D.A. McLennan. 1992. Phylogeny and

biodiversity:conserving our evolutionary legacy. Trends in Ecology and

Evolution 7:55-59.

George, A.L., D.A. Neely, R.L. Mayden. 2006. Conservation genetics of an imperiled

riverine fish from eastern North America, the blotchside logperch, Percina burtoni

(Teleostei: Percidae). Copeia 2006:585-594.

Goldchild, C.D. 1993. Status of the channel darter, Percina copelandi, in Canada. The

Canadian Field-Naturalist 107:431-439.

Khudamrongsawat, J., L.S. Heath, H.E. Heath, and P.M. Harris. 2007. Microsatellite

DNA primers for the endangered vermilion darter, Etheostoma chermocki, and

cross-species amplification in other darters (Percidae: Etheostoma). Molecular

Ecology Notes 7:811-813.

18 Kimura, M. 1980. A simple model for estimating evolutionary rates of base substitutions

through comparative studies of nucleotide sequences. Journal of Molecular

Evolution, 16, 111-120.

Li, L., P. Wang, C. Givens, S. Czesnys, and B. Brown. 2007. Isolation and

characterization of microsatellites in yellow (Perca flavescens). Molecular

Ecology Notes 7: 600-603.

McLennan, D.A. 1994. A phylogenetic approach to the evolution of fish behaviour.

Review in Fish Biology and Fisheries 4:430-460.

Moyle, P.B., and R.A. Leidy. 1992. Loss of biodiversity in aquatic ecosystems: evidence

from fish faunas. Pages 127-169 in P.L. Fiedler and S.K. Jain, eds. Conservation

biology: the theory and practice of nature conservation, preservation and

management. Chapman and Hall, New York.

Moritz, C. 1994. Defining “Evolutionarily Significant Units” for conservation. Trends in

Ecology and Evolution 9:373-375.

NatureServe. 2003. NatureServe Explorer: An online encyclopedia of life [web

application]. Version 1.8.

Near, T.J. 2002. Phylogenetic relationships of Percina (Percidae:).

Copeia 2002:1-14.

Near, T.J., L.M. Page, and R.L. Mayden. 2001. Intraspecific phylogeography of Percina

evides (Percidae: Etheostomatinae): an additional test of the Central Highlands

pre-Pleistocene vicariance hypothesis. Molecular Ecology 10:2235-2240.

19 Page, L.M., and G.S. Whitt. 1973. Lactate dehydrogenase isozymes of darters and the

inclusiveness of the genus Percina. Illinois Natural History Survey Biological

Notes 82:1-7.

Page, R. D. M. 1996. TREEVIEW: An application to display phylogenetic trees on

personal computers. Computer Applications in the Biosciences 12: 357-358

Ross, S.T. 2001. Inland fishes of Mississippi. University Press of Mississippi. Jackson.

624 pp.

Saitou N., Nei, M. 1987. The neighbor-joining method: a new method for reconstructing

phylogenetic trees. Molecular Biology and Evolution 4:406-425.

Schofield, P.J., and S.T. Ross. 2003. Habitat selection of the channel darter, Percina

(Cottogaster) copelandi, a surrogate for the imperiled pearl darter, Percina

aurora. Journal of Freshwater Ecology 18:249-257.

Shepard, T.E., P.E. O’Neil, M.F. Mettee, S.T. McGregor, and W.P. Henderson, Jr. 2002.

Status of the Cahaba shiner (Notropis cahabae), coal darter (Percina brevicauda),

and Tuskaloosa darter (Etheostoma douglasi) in the Locust Fork and Valley

Creek Systems, Alabama, 2001. Alabama Geological Survey Circular 201.

Slack, W.T., R.J. Heise, M.A. Dugo, and J.A. Ewing, III. 2002. Status of the pearl darter

(Percidae: Percina aurora) in the Pascagoula River, Mississippi. Final Project

Report, Contract Number 1448-40181-00-G-097.

Slack, W.T., M.A. Dugo, B.R. Kreiser, P. Mickle, J.S. Peyton, and R.L. Jones. 2005. A

survey of Upper Pascagoula drainage for the Pearl darter, Percina aurora Suttkus

and Thompson. Final Project Report No. 109, Contract Number E-1 Segment 19.

20 Sloss, B.L., N. Billington, B.M. Burr. 1994. A molecular phylogeny of the Percidae

(Teleostei, ) based on mitochondrial DNA sequence. Molecular

Phyogenetics and Evolution. 32: 545-562.

Song C.B., Near T.J. and L.M. Page. 1998. Phylogenetic relations among percid fishes

as inferred from mitochondrial cytochrome b DNA sequence data. Molecular

Phylogenetics and Evolution 10:343-53.

Suttkus, R.D., B.A. Thompson, and H.L. Bart,. Jr.. 1994. Two new darters, Percina

(Cottogaster), from the southeastern United States, with a review of the subgenus.

Occcasional Papers of the Tulane University Museum of Natural History, No. 4

Swofford, D.L. 2002. PAUP* 4.0: phylogenetic analysis using parsimony (* and other

methods). Ver. 4.b10. Sinauer Associates, Sunderland, MA.

Tonnis, B.D. 2006. Microsatellite DNA markers for the rainbow darter, Etheostoma

caeruleum (Percidae), and their potential utility for other darter species. Molecluar

Ecology Notes: 6:230-232.

Turner, T.F., J.C. Trexler, D.N. Kuhn, and H.W. Robison. 1996. Life-history variation

and the comparative phylogeography of darters (Pisces: Percidae) from the North

American Central Highlands. Evolution 50:2023-2036.

Waples, R.S. 1995. Evolutionary significant units and the conservation of biological

diversity under the Endangered Species Act. American Fisheries Society

Symposium 17:8-27.

Warren, M.L., Jr., B.M. Burr, S.J., Walsh, H.L. Bart, Jr., R.C., Cashner, D.A., Etnier, B.J.

Freeman, B.R. Kuhajda, R.L. Mayden, H.W. Robinson, S.T. Ross, and W.C.

21 Starnes. 2000. Diversity, distribution, and conservation status of the native

freshwater fishes of the southern United States. Fisheries 25:7-31.

Williams, J.E., R.R. Miller. 1990. Conservation status of the North American fish fauna

in fresh water. Journal of Fish Biology 37 (Supplement A): 79-85.

22 Appendix A. Collection Data for DNA Extractions, Percina aurora.

MISSISSIPPI MUSEUM OF NATURAL SCIENCE DNA Tissue Collection Collection Data for DNA Tissue deposted in MMNS Tissue Collection.

FAMILY: Percidae TAXA: Percina aurora

TOTAL NUMBER OF COLLECTIONS: 24

STATE: Mississippi COUNTY: Clarke MMNS TISSUE CATALOG NUMBER: 190.03 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Chickasawhay River WATER: Chickasawhay River LOCALE: RDB, 4.5 km downstream of Stonewall boat launch UTMX: 329357 UTMY: 3553981 LATdd: 32.111 LONGdd: -88.8086 FIELD NUMBER: WTS04-019 COLLECTORS: W.T. Slack, M.A. Dugo, J.S. Peyton COLLECTION DATE: 8 / 18 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

STATE: Mississippi COUNTY: Clarke MMNS TISSUE CATALOG NUMBER: 180.01 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Chickasawhay River WATER: Chickasawhay River LOCALE: RDB and midchannel gravel island, 500 m upstream from bridge crossing W of Stonewall UTMX: 329147 UTMY: 3556545 LATdd: 32.1341 LONGdd: -88.8113 FIELD NUMBER: WTS04-014 COLLECTORS: W.T. Slack, M.A. Dugo, J.S. Peyton COLLECTION DATE: 8 / 17 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

STATE: Mississippi COUNTY: Clarke MMNS TISSUE CATALOG NUMBER: 180.02 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Chickasawhay River WATER: Chickasawhay River LOCALE: RDB and midchannel gravel island, 500 m upstream from bridge crossing W of Stonewall UTMX: 329147 UTMY: 3556545 LATdd: 32.1341 LONGdd: -88.8113 FIELD NUMBER: WTS04-014 COLLECTORS: W.T. Slack, M.A. Dugo, J.S. Peyton COLLECTION DATE: 8 / 17 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

23 Appendix A. Collection Data for DNA Extractions, Percina aurora.

STATE: Mississippi COUNTY: Clarke MMNS TISSUE CATALOG NUMBER: 180.03 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Chickasawhay River WATER: Chickasawhay River LOCALE: RDB and midchannel gravel island, 500 m upstream from bridge crossing W of Stonewall UTMX: 329147 UTMY: 3556545 LATdd: 32.1341 LONGdd: -88.8113 FIELD NUMBER: WTS04-014 COLLECTORS: W.T. Slack, M.A. Dugo, J.S. Peyton COLLECTION DATE: 8 / 17 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

STATE: Mississippi COUNTY: Clarke MMNS TISSUE CATALOG NUMBER: 190.02 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Chickasawhay River WATER: Chickasawhay River LOCALE: RDB, 4.5 km downstream of Stonewall boat launch UTMX: 329357 UTMY: 3553981 LATdd: 32.111 LONGdd: -88.8086 FIELD NUMBER: WTS04-019 COLLECTORS: W.T. Slack, M.A. Dugo, J.S. Peyton COLLECTION DATE: 8 / 18 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

STATE: Mississippi COUNTY: Clarke MMNS TISSUE CATALOG NUMBER: 190.04 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Chickasawhay River WATER: Chickasawhay River LOCALE: RDB, 4.5 km downstream of Stonewall boat launch UTMX: 329357 UTMY: 3553981 LATdd: 32.111 LONGdd: -88.8086 FIELD NUMBER: WTS04-019 COLLECTORS: W.T. Slack, M.A. Dugo, J.S. Peyton COLLECTION DATE: 8 / 18 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

STATE: Mississippi COUNTY: Clarke MMNS TISSUE CATALOG NUMBER: 190.05 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Chickasawhay River WATER: Chickasawhay River LOCALE: RDB, 4.5 km downstream of Stonewall boat launch UTMX: 329357 UTMY: 3553981 LATdd: 32.111 LONGdd: -88.8086 FIELD NUMBER: WTS04-019 COLLECTORS: W.T. Slack, M.A. Dugo, J.S. Peyton COLLECTION DATE: 8 / 18 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

24 Appendix A. Collection Data for DNA Extractions, Percina aurora.

STATE: Mississippi COUNTY: Clarke MMNS TISSUE CATALOG NUMBER: 204.01 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Chickasawhay River WATER: Chickasawhay River LOCALE: LDB, 4.0 km upstream of Desoto boat launch (CR 690) UTMX: 337782 UTMY: 3541515 LATdd: 31.9998 LONGdd: -88.7173 FIELD NUMBER: WTS04-038 COLLECTORS: W.T. Slack, M.A. Dugo, M. Wilson, J.S. Peyton, R.L. Jones, R. Rummel COLLECTION DATE: 9 / 2 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

STATE: Mississippi COUNTY: Clarke MMNS TISSUE CATALOG NUMBER: 205.02 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Chickasawhay River WATER: Chickasawhay River LOCALE: LDB, 1.5 km upstream of Desoto boat launch (CR 690) UTMX: 338331 UTMY: 3539402 LATdd: 31.9808 LONGdd: -88.7111 FIELD NUMBER: WTS04-037 COLLECTORS: W.T. Slack, M.A. Dugo, M. Wilson, J.S. Peyton, R.L. Jones, R. Rummel COLLECTION DATE: 9 / 2 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

STATE: Mississippi COUNTY: Clarke MMNS TISSUE CATALOG NUMBER: 207.01 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Chickasawhay River WATER: Chickasawhay River LOCALE: RDB, 7.5 km upstream of Desoto boat launch (CR 690); 500 m S of Archusa Water Park UTMX: 337701 UTMY: 3543720 LATdd: 32.0197 LONGdd: -88.7185 FIELD NUMBER: WTS04-040 COLLECTORS: W.T. Slack, M.A. Dugo, M. Wilson, J.S. Peyton, R.L. Jones, R. Rummel COLLECTION DATE: 9 / 2 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

STATE: Mississippi COUNTY: Clarke MMNS TISSUE CATALOG NUMBER: 207.02 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Chickasawhay River WATER: Chickasawhay River LOCALE: RDB, 7.5 km upstream of Desoto boat launch (CR 690); 500 m S of Archusa Water Park UTMX: 337701 UTMY: 3543720 LATdd: 32.0197 LONGdd: -88.7185 FIELD NUMBER: WTS04-040 COLLECTORS: W.T. Slack, M.A. Dugo, M. Wilson, J.S. Peyton, R.L. Jones, R. Rummel COLLECTION DATE: 9 / 2 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

25 Appendix A. Collection Data for DNA Extractions, Percina aurora.

STATE: Mississippi COUNTY: Clarke MMNS TISSUE CATALOG NUMBER: 207.03 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Chickasawhay River WATER: Chickasawhay River LOCALE: RDB, 7.5 km upstream of Desoto boat launch (CR 690); 500 m S of Archusa Water Park UTMX: 337701 UTMY: 3543720 LATdd: 32.0197 LONGdd: -88.7185 FIELD NUMBER: WTS04-040 COLLECTORS: W.T. Slack, M.A. Dugo, M. Wilson, J.S. Peyton, R.L. Jones, R. Rummel COLLECTION DATE: 9 / 2 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

STATE: Mississippi COUNTY: Clarke MMNS TISSUE CATALOG NUMBER: 205.01 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Chickasawhay River WATER: Chickasawhay River LOCALE: LDB, 1.5 km upstream of Desoto boat launch (CR 690) UTMX: 338331 UTMY: 3539402 LATdd: 31.9808 LONGdd: -88.7111 FIELD NUMBER: WTS04-037 COLLECTORS: W.T. Slack, M.A. Dugo, M. Wilson, J.S. Peyton, R.L. Jones, R. Rummel COLLECTION DATE: 9 / 2 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

STATE: Mississippi COUNTY: Clarke MMNS TISSUE CATALOG NUMBER: 190.01 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Chickasawhay River WATER: Chickasawhay River LOCALE: RDB, 4.5 km downstream of Stonewall boat launch UTMX: 329357 UTMY: 3553981 LATdd: 32.111 LONGdd: -88.8086 FIELD NUMBER: WTS04-019 COLLECTORS: W.T. Slack, M.A. Dugo, J.S. Peyton COLLECTION DATE: 8 / 18 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

STATE: Mississippi COUNTY: George MMNS TISSUE CATALOG NUMBER: 102.02 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Pascagoula River WATER: Pascagoula River LOCALE: RDB-W of Pierce Lake at Pascagoula River Estates UTMX: 333054 UTMY: 3413627 LATdd: 30.8457 LONGdd: -88.7458 FIELD NUMBER: WTS03-033 COLLECTORS: W.T. Slack, M.A. Dugo COLLECTION DATE: 9 / 18 / 2003 PRESERVATION: SED Buffer; 100% DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 31559.0000 VOUCHER LOCATION: MMNS COMMENTS:

26 Appendix A. Collection Data for DNA Extractions, Percina aurora.

STATE: Mississippi COUNTY: George MMNS TISSUE CATALOG NUMBER: 102.01 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Pascagoula River WATER: Pascagoula River LOCALE: RDB-W of Pierce Lake at Pascagoula River Estates UTMX: 333054 UTMY: 3413627 LATdd: 30.8457 LONGdd: -88.7458 FIELD NUMBER: WTS03-033 COLLECTORS: W.T. Slack, M.A. Dugo COLLECTION DATE: 9 / 18 / 2003 PRESERVATION: SED Buffer; 100% DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 31559.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Mississippi COUNTY: Greene MMNS TISSUE CATALOG NUMBER: 171.05 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Leaf River WATER: Leaf River LOCALE: LDB, 3.5 km upstream of Merrill boat launch at upper end of Cowens Dead River UTMX: 333042 UTMY: 3431942 LATdd: 31.0109 LONGdd: -88.7489 FIELD NUMBER: WTS04-002 COLLECTORS: W.T. Slack, M.A. Dugo, B.R. Kreiser, P. Mickle COLLECTION DATE: 8 / 12 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 43042.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Mississippi COUNTY: Greene MMNS TISSUE CATALOG NUMBER: 171.01 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Leaf River WATER: Leaf River LOCALE: LDB, 3.5 km upstream of Merrill boat launch at upper end of Cowens Dead River UTMX: 333042 UTMY: 3431942 LATdd: 31.0109 LONGdd: -88.7489 FIELD NUMBER: WTS04-002 COLLECTORS: W.T. Slack, M.A. Dugo, B.R. Kreiser, P. Mickle COLLECTION DATE: 8 / 12 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 43042.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Mississippi COUNTY: Greene MMNS TISSUE CATALOG NUMBER: 171.04 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Leaf River WATER: Leaf River LOCALE: LDB, 3.5 km upstream of Merrill boat launch at upper end of Cowens Dead River UTMX: 333042 UTMY: 3431942 LATdd: 31.0109 LONGdd: -88.7489 FIELD NUMBER: WTS04-002 COLLECTORS: W.T. Slack, M.A. Dugo, B.R. Kreiser, P. Mickle COLLECTION DATE: 8 / 12 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 43042.0000 VOUCHER LOCATION: MMNS COMMENTS:

27 Appendix A. Collection Data for DNA Extractions, Percina aurora.

STATE: Mississippi COUNTY: Greene MMNS TISSUE CATALOG NUMBER: 171.03 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Leaf River WATER: Leaf River LOCALE: LDB, 3.5 km upstream of Merrill boat launch at upper end of Cowens Dead River UTMX: 333042 UTMY: 3431942 LATdd: 31.0109 LONGdd: -88.7489 FIELD NUMBER: WTS04-002 COLLECTORS: W.T. Slack, M.A. Dugo, B.R. Kreiser, P. Mickle COLLECTION DATE: 8 / 12 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 43042.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Mississippi COUNTY: Greene MMNS TISSUE CATALOG NUMBER: 171.02 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Leaf River WATER: Leaf River LOCALE: LDB, 3.5 km upstream of Merrill boat launch at upper end of Cowens Dead River UTMX: 333042 UTMY: 3431942 LATdd: 31.0109 LONGdd: -88.7489 FIELD NUMBER: WTS04-002 COLLECTORS: W.T. Slack, M.A. Dugo, B.R. Kreiser, P. Mickle COLLECTION DATE: 8 / 12 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 43042.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Mississippi COUNTY: Perry MMNS TISSUE CATALOG NUMBER: 640.01 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Leaf River WATER: Leaf River LOCALE: RDB, 8.0 rkm upstream of McLain boat ramp UTMX: 324089 UTMY: 3447168 LATdd: 31.14594 LONGdd: -88.84531 FIELD NUMBER: PM04-107 COLLECTORS: P. Mickle, K. Persons, B. Kreiser COLLECTION DATE: 9 / 2 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

STATE: Mississippi COUNTY: Perry MMNS TISSUE CATALOG NUMBER: 641.01 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Leaf River WATER: Leaf River LOCALE: RDB, 3.0 rkm downstream of Beaumont boat ramp UTMX: 320117 UTMY: 3451443 LATdd: 31.18489 LONGdd: -88.88773 FIELD NUMBER: PM04-108 COLLECTORS: P. Mickle, S. Jackson, J. Spaeth COLLECTION DATE: 9 / 6 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

28 Appendix A. Collection Data for DNA Extractions, Percina aurora.

STATE: Mississippi COUNTY: Perry MMNS TISSUE CATALOG NUMBER: 640.02 BASIN: Gulf of Mexico DRAINAGE: Pascagoula SYSTEM: Leaf River WATER: Leaf River LOCALE: RDB, 8.0 rkm upstream of McLain boat ramp UTMX: 324089 UTMY: 3447168 LATdd: 31.14594 LONGdd: -88.84531 FIELD NUMBER: PM04-107 COLLECTORS: P. Mickle, K. Persons, B. Kreiser COLLECTION DATE: 9 / 2 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

29 Appendix B. Collection Data for DNA Extractions, Percina brevicauda.

MISSISSIPPI MUSEUM OF NATURAL SCIENCE DNA Tissue Collection Collection Data for DNA Tissue deposted in MMNS Tissue Collection.

FAMILY: Percidae TAXA: Percina brevicauda

TOTAL NUMBER OF COLLECTIONS: 27

STATE: Alabama COUNTY: Bibb MMNS TISSUE CATALOG NUMBER: 877 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Cahaba WATER: Cahaba River LOCALE: HWY 26 bridge, 8.5 mi N. of Centreville UTMX: 492706 UTMY: 3653029 LATdd: 33.0174 LONGdd: -87.0781 FIELD NUMBER: MAD07-007 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 07 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50514.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Alabama COUNTY: Blount MMNS TISSUE CATALOG NUMBER: 367.01 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Black Warrior River WATER: Locust Fork LOCALE: Abandoned Dean's Ferry Bridge, N of town of County Line UTMX: 525207 UTMY: 3745001 LATdd: 33.8467 LONGdd: -86.7275 FIELD NUMBER: MAD05-095 COLLECTORS: M.A. Dugo, W.T. Slack, B.R. Kreiser COLLECTION DATE: 6 / 29 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: MMNS COMMENTS:

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 876.04 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: Kings Bridge (@ USGS gauging Station) UTMX: 568327 UTMY: 3642133 LATdd: 32.917 LONGdd: -86.2973 FIELD NUMBER: MAD07-006 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50514.0000 VOUCHER LOCATION: MMNS COMMENTS:

30 Appendix B. Collection Data for DNA Extractions, Percina brevicauda.

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 875.01 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: HWY 231 bridge crossing UTMX: 574466 UTMY: 3645088 LATdd: 32.9433 LONGdd: -86.2034 FIELD NUMBER: MAD07-004 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50528.0000 VOUCHER LOCATION: MMNS COMMENTS: P. brevicauda collected in three seine hauls

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 876.01 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: Kings Bridge (@ USGS gauging Station) UTMX: 568327 UTMY: 3642133 LATdd: 32.917 LONGdd: -86.2973 FIELD NUMBER: MAD07-006 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50514.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 876.03 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: Kings Bridge (@ USGS gauging Station) UTMX: 568327 UTMY: 3642133 LATdd: 32.917 LONGdd: -86.2973 FIELD NUMBER: MAD07-006 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50514.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 876.05 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: Kings Bridge (@ USGS gauging Station) UTMX: 568327 UTMY: 3642133 LATdd: 32.917 LONGdd: -86.2973 FIELD NUMBER: MAD07-006 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50514.0000 VOUCHER LOCATION: MMNS COMMENTS:

31 Appendix B. Collection Data for DNA Extractions, Percina brevicauda.

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 875.02 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: HWY 231 bridge crossing UTMX: 574466 UTMY: 3645088 LATdd: 32.9433 LONGdd: -86.2034 FIELD NUMBER: MAD07-004 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50528.0000 VOUCHER LOCATION: MMNS COMMENTS: P. brevicauda collected in three seine hauls

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 875.03 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: HWY 231 bridge crossing UTMX: 574466 UTMY: 3645088 LATdd: 32.9433 LONGdd: -86.2034 FIELD NUMBER: MAD07-004 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50528.0000 VOUCHER LOCATION: MMNS COMMENTS: P. brevicauda collected in three seine hauls

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 875.1 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: HWY 231 bridge crossing UTMX: 574466 UTMY: 3645088 LATdd: 32.9433 LONGdd: -86.2034 FIELD NUMBER: MAD07-004 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50528.0000 VOUCHER LOCATION: MMNS COMMENTS: P. brevicauda collected in three seine hauls (sampling included multiple

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 875.05 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: HWY 231 bridge crossing UTMX: 574466 UTMY: 3645088 LATdd: 32.9433 LONGdd: -86.2034 FIELD NUMBER: MAD07-004 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50528.0000 VOUCHER LOCATION: MMNS COMMENTS: P. brevicauda collected in three seine hauls

32 Appendix B. Collection Data for DNA Extractions, Percina brevicauda.

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 875.06 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: HWY 231 bridge crossing UTMX: 574466 UTMY: 3645088 LATdd: 32.9433 LONGdd: -86.2034 FIELD NUMBER: MAD07-004 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50528.0000 VOUCHER LOCATION: MMNS COMMENTS: P. brevicauda collected in three seine hauls

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 875.07 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: HWY 231 bridge crossing UTMX: 574466 UTMY: 3645088 LATdd: 32.9433 LONGdd: -86.2034 FIELD NUMBER: MAD07-004 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50528.0000 VOUCHER LOCATION: MMNS COMMENTS: P. brevicauda collected in three seine hauls

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 875.08 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: HWY 231 bridge crossing UTMX: 574466 UTMY: 3645088 LATdd: 32.9433 LONGdd: -86.2034 FIELD NUMBER: MAD07-004 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50528.0000 VOUCHER LOCATION: MMNS COMMENTS: P. brevicauda collected in three seine hauls

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 876.02 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: Kings Bridge (@ USGS gauging Station) UTMX: 568327 UTMY: 3642133 LATdd: 32.917 LONGdd: -86.2973 FIELD NUMBER: MAD07-006 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50514.0000 VOUCHER LOCATION: MMNS COMMENTS:

33 Appendix B. Collection Data for DNA Extractions, Percina brevicauda.

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 875.09 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: HWY 231 bridge crossing UTMX: 574466 UTMY: 3645088 LATdd: 32.9433 LONGdd: -86.2034 FIELD NUMBER: MAD07-004 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50528.0000 VOUCHER LOCATION: MMNS COMMENTS: P. brevicauda collected in three seine hauls

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 875.04 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Coosa WATER: Hatchet Creek LOCALE: HWY 231 bridge crossing UTMX: 574466 UTMY: 3645088 LATdd: 32.9433 LONGdd: -86.2034 FIELD NUMBER: MAD07-004 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 06 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 50528.0000 VOUCHER LOCATION: MMNS COMMENTS: P. brevicauda collected in three seine hauls

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 603.01 BASIN: Mobile DRAINAGE: Coosa SYSTEM: Hatchet Creek WATER: Hatchet Creek LOCALE: Hwy 231 bridge crossing UTMX: 574446 UTMY: 3645359 LATdd: 32.944 LONGdd: -86.2036 FIELD NUMBER: COLLECTORS: Carol Johnston COLLECTION DATE: 10 / 17 / 2000 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 39481.0000 VOUCHER LOCATION: AU COMMENTS: Donated from C. Johnston - Fall 2004. Labeled # 3 and 4. Used all tissue

STATE: Alabama COUNTY: Coosa MMNS TISSUE CATALOG NUMBER: 603.02 BASIN: Mobile DRAINAGE: Coosa SYSTEM: Hatchet Creek WATER: Hatchet Creek LOCALE: Hwy 231 bridge crossing UTMX: 574446 UTMY: 3645359 LATdd: 32.944 LONGdd: -86.2036 FIELD NUMBER: COLLECTORS: Carol Johnston COLLECTION DATE: 10 / 17 / 2000 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 39481.0000 VOUCHER LOCATION: AU COMMENTS: Donated from C. Johnston - Fall 2004. Labeled # 3 and 4. Used all tissue

34 Appendix B. Collection Data for DNA Extractions, Percina brevicauda.

STATE: Alabama COUNTY: Jefferson MMNS TISSUE CATALOG NUMBER: 417.02 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Black Warrior River WATER: Locust Fork LOCALE: Warrior - Kimberly Rd. bridge crossing UTMX: 518423 UTMY: 3740786 LATdd: 33.8089 LONGdd: -86.801 FIELD NUMBER: MAD05-094 COLLECTORS: M.A. Dugo, W.T. Slack, B.R. Kreiser COLLECTION DATE: 6 / 29 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: MMNS COMMENTS:

STATE: Alabama COUNTY: Jefferson MMNS TISSUE CATALOG NUMBER: 417.01 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Black Warrior River WATER: Locust Fork LOCALE: Warrior - Kimberly Rd. bridge crossing UTMX: 518423 UTMY: 3740786 LATdd: 33.8089 LONGdd: -86.801 FIELD NUMBER: MAD05-094 COLLECTORS: M.A. Dugo, W.T. Slack, B.R. Kreiser COLLECTION DATE: 6 / 29 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: MMNS COMMENTS:

STATE: Alabama COUNTY: Shelby MMNS TISSUE CATALOG NUMBER: 878.01 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Cahaba WATER: Cahaba River LOCALE: Hwy 52, 3.2 miles W of Helana UTMX: 510878 UTMY: 3682578 LATdd: 33.2839 LONGdd: -86.8832 FIELD NUMBER: MAD07-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 07 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

STATE: Alabama COUNTY: Shelby MMNS TISSUE CATALOG NUMBER: 878.03 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Cahaba WATER: Cahaba River LOCALE: Hwy 52, 3.2 miles W of Helana UTMX: 510878 UTMY: 3682578 LATdd: 33.2839 LONGdd: -86.8832 FIELD NUMBER: MAD07-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 07 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

35 Appendix B. Collection Data for DNA Extractions, Percina brevicauda.

STATE: Alabama COUNTY: Shelby MMNS TISSUE CATALOG NUMBER: 878.02 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Cahaba WATER: Cahaba River LOCALE: Hwy 52, 3.2 miles W of Helana UTMX: 510878 UTMY: 3682578 LATdd: 33.2839 LONGdd: -86.8832 FIELD NUMBER: MAD07-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 9 / 07 / 2007 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: COMMENTS:

STATE: Alabama COUNTY: Shelby MMNS TISSUE CATALOG NUMBER: 418.03 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Cahaba River WATER: Cahaba River LOCALE: Hwy 57 bridge crossing, W of Alabaster UTMX: 510882 UTMY: 3682545 LATdd: 33.2836 LONGdd: -86.8831 FIELD NUMBER: MAD05-093 COLLECTORS: M.A. Dugo, W.T. Slack, B.R. Kreiser COLLECTION DATE: 6 / 29 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: MMNS COMMENTS:

STATE: Alabama COUNTY: Shelby MMNS TISSUE CATALOG NUMBER: 418.02 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Cahaba River WATER: Cahaba River LOCALE: Hwy 57 bridge crossing, W of Alabaster UTMX: 510882 UTMY: 3682545 LATdd: 33.2836 LONGdd: -86.8831 FIELD NUMBER: MAD05-093 COLLECTORS: M.A. Dugo, W.T. Slack, B.R. Kreiser COLLECTION DATE: 6 / 29 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: MMNS COMMENTS:

STATE: Alabama COUNTY: Shelby MMNS TISSUE CATALOG NUMBER: 418.01 BASIN: Gulf of Mexico DRAINAGE: Mobile SYSTEM: Cahaba River WATER: Cahaba River LOCALE: Hwy 57 bridge crossing, W of Alabaster UTMX: 510882 UTMY: 3682545 LATdd: 33.2836 LONGdd: -86.8831 FIELD NUMBER: MAD05-093 COLLECTORS: M.A. Dugo, W.T. Slack, B.R. Kreiser COLLECTION DATE: 6 / 29 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: VOUCHER LOCATION: MMNS COMMENTS:

36 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

MISSISSIPPI MUSEUM OF NATURAL SCIENCE DNA Tissue Collection Collection Data for DNA Tissue deposted in MMNS Tissue Collection.

FAMILY: Percidae TAXA: Percina copelandi

TOTAL NUMBER OF COLLECTIONS: 96

STATE: Arkansas COUNTY: Clark MMNS TISSUE CATALOG NUMBER: 145.02 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Caddo River WATER: Caddo River LOCALE: @ State Rd. 82, 2 m N of Amity UTMX: 457447 UTMY: 3793401 LATdd: 34.2827 LONGdd: -93.4623 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 26 / 2001 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 37938.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Clark MMNS TISSUE CATALOG NUMBER: 145.01 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Caddo River WATER: Caddo River LOCALE: @ State Rd. 82, 2 m N of Amity UTMX: 457447 UTMY: 3793401 LATdd: 34.2827 LONGdd: -93.4623 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 26 / 2001 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 37938.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Grant MMNS TISSUE CATALOG NUMBER: 146.07 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Saline River WATER: Saline River LOCALE: @ US Hwy 270, 1.5 mi W of Prattville UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 30 / 2001 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40192.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

37 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Arkansas COUNTY: Grant MMNS TISSUE CATALOG NUMBER: 146.08 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Saline River WATER: Saline River LOCALE: @ US Hwy 270, 1.5 mi W of Prattville UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 30 / 2001 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40192.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Grant MMNS TISSUE CATALOG NUMBER: 146.09 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Saline River WATER: Saline River LOCALE: @ US Hwy 270, 1.5 mi W of Prattville UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 30 / 2001 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40192.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Grant MMNS TISSUE CATALOG NUMBER: 146.1 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Saline River WATER: Saline River LOCALE: @ US Hwy 270, 1.5 mi W of Prattville UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 30 / 2001 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40192.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Grant MMNS TISSUE CATALOG NUMBER: 146.06 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Saline River WATER: Saline River LOCALE: @ US Hwy 270, 1.5 mi W of Prattville UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 30 / 2001 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40192.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

38 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Arkansas COUNTY: Grant MMNS TISSUE CATALOG NUMBER: 146.05 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Saline River WATER: Saline River LOCALE: @ US Hwy 270, 1.5 mi W of Prattville UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 30 / 2001 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40192.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Grant MMNS TISSUE CATALOG NUMBER: 146.04 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Saline River WATER: Saline River LOCALE: @ US Hwy 270, 1.5 mi W of Prattville UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 30 / 2001 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40192.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Grant MMNS TISSUE CATALOG NUMBER: 146.03 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Saline River WATER: Saline River LOCALE: @ US Hwy 270, 1.5 mi W of Prattville UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 30 / 2001 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40192.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Grant MMNS TISSUE CATALOG NUMBER: 146.02 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Saline River WATER: Saline River LOCALE: @ US Hwy 270, 1.5 mi W of Prattville UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 30 / 2001 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40192.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

39 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Arkansas COUNTY: Grant MMNS TISSUE CATALOG NUMBER: 146.01 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Saline River WATER: Saline River LOCALE: @ US Hwy 270, 1.5 mi W of Prattville UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 30 / 2001 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40192.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 144.07 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Caddo River WATER: Caddo River LOCALE: Caddo River at Caddo Camp UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 29 / 2002 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40435.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 144.02 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Caddo River WATER: Caddo River LOCALE: Caddo River at Caddo Camp UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 29 / 2002 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40435.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 149.02 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Caddo River WATER: Caddo River LOCALE: Caddo River @ canoe acces, upstream of Hwy 240 UTMX: 439848 UTMY: 3693255 LATdd: 33.3786 LONGdd: -99.6467 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 3 / 19 / 2002 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 44106.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

40 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 144.08 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Caddo River WATER: Caddo River LOCALE: Caddo River at Caddo Camp UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 29 / 2002 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40435.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 149.01 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Caddo River WATER: Caddo River LOCALE: Caddo River @ canoe acces, upstream of Hwy 240 UTMX: 439848 UTMY: 3693255 LATdd: 33.3786 LONGdd: -99.6467 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 3 / 19 / 2002 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 44106.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 144.06 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Caddo River WATER: Caddo River LOCALE: Caddo River at Caddo Camp UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 29 / 2002 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40435.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 144.05 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Caddo River WATER: Caddo River LOCALE: Caddo River at Caddo Camp UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 29 / 2002 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40435.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

41 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 144.04 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Caddo River WATER: Caddo River LOCALE: Caddo River at Caddo Camp UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 29 / 2002 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40435.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 144.03 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Caddo River WATER: Caddo River LOCALE: Caddo River at Caddo Camp UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 29 / 2002 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40435.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 144.01 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Caddo River WATER: Caddo River LOCALE: Caddo River at Caddo Camp UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 29 / 2002 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40435.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 144.09 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Caddo River WATER: Caddo River LOCALE: Caddo River at Caddo Camp UTMX: 537699 UTMY: 3796624 LATdd: 34.3119 LONGdd: -92.5903 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 5 / 29 / 2002 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 40435.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

42 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 148.08 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Ouachita River WATER: Ouachita River LOCALE: Ouachita River @ Hwy 379, 0.5 mi. S of Oden UTMX: 425402 UTMY: 3832857 LATdd: 34.6367 LONGdd: -93.8139 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 3 / 16 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 42406.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 148.07 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Ouachita River WATER: Ouachita River LOCALE: Ouachita River @ Hwy 379, 0.5 mi. S of Oden UTMX: 425402 UTMY: 3832857 LATdd: 34.6367 LONGdd: -93.8139 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 3 / 16 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 42406.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 148.06 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Ouachita River WATER: Ouachita River LOCALE: Ouachita River @ Hwy 379, 0.5 mi. S of Oden UTMX: 425402 UTMY: 3832857 LATdd: 34.6367 LONGdd: -93.8139 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 3 / 16 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 42406.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 148.01 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Ouachita River WATER: Ouachita River LOCALE: Ouachita River @ Hwy 379, 0.5 mi. S of Oden UTMX: 425402 UTMY: 3832857 LATdd: 34.6367 LONGdd: -93.8139 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 3 / 16 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 42406.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

43 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 148.02 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Ouachita River WATER: Ouachita River LOCALE: Ouachita River @ Hwy 379, 0.5 mi. S of Oden UTMX: 425402 UTMY: 3832857 LATdd: 34.6367 LONGdd: -93.8139 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 3 / 16 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 42406.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 148.03 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Ouachita River WATER: Ouachita River LOCALE: Ouachita River @ Hwy 379, 0.5 mi. S of Oden UTMX: 425402 UTMY: 3832857 LATdd: 34.6367 LONGdd: -93.8139 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 3 / 16 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 42406.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 148.04 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Ouachita River WATER: Ouachita River LOCALE: Ouachita River @ Hwy 379, 0.5 mi. S of Oden UTMX: 425402 UTMY: 3832857 LATdd: 34.6367 LONGdd: -93.8139 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 3 / 16 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 42406.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 148.05 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Ouachita River WATER: Ouachita River LOCALE: Ouachita River @ Hwy 379, 0.5 mi. S of Oden UTMX: 425402 UTMY: 3832857 LATdd: 34.6367 LONGdd: -93.8139 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 3 / 16 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 42406.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

44 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Arkansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 148.09 BASIN: Mississippi River DRAINAGE: Ouachita River SYSTEM: Ouachita River WATER: Ouachita River LOCALE: Ouachita River @ Hwy 379, 0.5 mi. S of Oden UTMX: 425402 UTMY: 3832857 LATdd: 33.3786 LONGdd: -93.8139 FIELD NUMBER: COLLECTORS: COLLECTION DATE: 3 / 16 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 42406.0000 VOUCHER LOCATION: JFBM COMMENTS: Donated by Andrew Simons, U of Minnesota, 28-March 2006

STATE: Kansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 700.09 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Verdigris River LOCALE: County Road 5800 bridge crossing, E of Sycamore UTMX: 262197 UTMY: 4134314 LATdd: 37.3269 LONGdd: -95.6839 FIELD NUMBER: MAD06-016 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 12 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47858.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Kansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 700.1 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Verdigris River LOCALE: County Road 5800 bridge crossing, E of Sycamore UTMX: 262197 UTMY: 4134314 LATdd: 37.3269 LONGdd: -95.6839 FIELD NUMBER: MAD06-016 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 12 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47858.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Kansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 700.08 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Verdigris River LOCALE: County Road 5800 bridge crossing, E of Sycamore UTMX: 262197 UTMY: 4134314 LATdd: 37.3269 LONGdd: -95.6839 FIELD NUMBER: MAD06-016 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 12 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47858.0000 VOUCHER LOCATION: MMNS COMMENTS:

45 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Kansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 700.07 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Verdigris River LOCALE: County Road 5800 bridge crossing, E of Sycamore UTMX: 262197 UTMY: 4134314 LATdd: 37.3269 LONGdd: -95.6839 FIELD NUMBER: MAD06-016 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 12 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47858.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Kansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 700.06 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Verdigris River LOCALE: County Road 5800 bridge crossing, E of Sycamore UTMX: 262197 UTMY: 4134314 LATdd: 37.3269 LONGdd: -95.6839 FIELD NUMBER: MAD06-016 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 12 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47858.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Kansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 700.05 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Verdigris River LOCALE: County Road 5800 bridge crossing, E of Sycamore UTMX: 262197 UTMY: 4134314 LATdd: 37.3269 LONGdd: -95.6839 FIELD NUMBER: MAD06-016 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 12 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47858.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Kansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 700.04 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Verdigris River LOCALE: County Road 5800 bridge crossing, E of Sycamore UTMX: 262197 UTMY: 4134314 LATdd: 37.3269 LONGdd: -95.6839 FIELD NUMBER: MAD06-016 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 12 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47858.0000 VOUCHER LOCATION: MMNS COMMENTS:

46 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Kansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 700.03 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Verdigris River LOCALE: County Road 5800 bridge crossing, E of Sycamore UTMX: 262197 UTMY: 4134314 LATdd: 37.3269 LONGdd: -95.6839 FIELD NUMBER: MAD06-016 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 12 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47858.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Kansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 700.02 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Verdigris River LOCALE: County Road 5800 bridge crossing, E of Sycamore UTMX: 262197 UTMY: 4134314 LATdd: 37.3269 LONGdd: -95.6839 FIELD NUMBER: MAD06-016 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 12 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47858.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Kansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 700.01 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Verdigris River LOCALE: County Road 5800 bridge crossing, E of Sycamore UTMX: 262197 UTMY: 4134314 LATdd: 37.3269 LONGdd: -95.6839 FIELD NUMBER: MAD06-016 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 12 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47858.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Kansas COUNTY: Montgomery MMNS TISSUE CATALOG NUMBER: 700.11 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Verdigris River LOCALE: County Road 5800 bridge crossing, E of Sycamore UTMX: 262197 UTMY: 4134314 LATdd: 37.3269 LONGdd: -95.6839 FIELD NUMBER: MAD06-016 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 12 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47858.0000 VOUCHER LOCATION: MMNS COMMENTS:

47 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Kentucky COUNTY: Hart MMNS TISSUE CATALOG NUMBER: 281.06 BASIN: Mississippi River DRAINAGE: Ohio SYSTEM: Green River WATER: Green River LOCALE: at first island upstream of US31W bridge at Munfordville UTMX: 598948 UTMY: 4125110 LATdd: 37.2692 LONGdd: -85.884 FIELD NUMBER: COLLECTORS: M.R. Thomas, J.G. Stewart, D. Zeman, B. Zeman, R.R. Cicerello COLLECTION DATE: 7 / 13 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Kentucky COUNTY: Hart MMNS TISSUE CATALOG NUMBER: 281.05 BASIN: Mississippi River DRAINAGE: Ohio SYSTEM: Green River WATER: Green River LOCALE: at first island upstream of US31W bridge at Munfordville UTMX: 598948 UTMY: 4125110 LATdd: 37.2692 LONGdd: -85.884 FIELD NUMBER: COLLECTORS: M.R. Thomas, J.G. Stewart, D. Zeman, B. Zeman, R.R. Cicerello COLLECTION DATE: 7 / 13 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Kentucky COUNTY: Hart MMNS TISSUE CATALOG NUMBER: 281.04 BASIN: Mississippi River DRAINAGE: Ohio SYSTEM: Green River WATER: Green River LOCALE: at first island upstream of US31W bridge at Munfordville UTMX: 598948 UTMY: 4125110 LATdd: 37.2692 LONGdd: -85.884 FIELD NUMBER: COLLECTORS: M.R. Thomas, J.G. Stewart, D. Zeman, B. Zeman, R.R. Cicerello COLLECTION DATE: 7 / 13 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Kentucky COUNTY: Hart MMNS TISSUE CATALOG NUMBER: 281.03 BASIN: Mississippi River DRAINAGE: Ohio SYSTEM: Green River WATER: Green River LOCALE: at first island upstream of US31W bridge at Munfordville UTMX: 598948 UTMY: 4125110 LATdd: 37.2692 LONGdd: -85.884 FIELD NUMBER: COLLECTORS: M.R. Thomas, J.G. Stewart, D. Zeman, B. Zeman, R.R. Cicerello COLLECTION DATE: 7 / 13 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: MMNS COMMENTS:

48 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Kentucky COUNTY: Hart MMNS TISSUE CATALOG NUMBER: 281.02 BASIN: Mississippi River DRAINAGE: Ohio SYSTEM: Green River WATER: Green River LOCALE: at first island upstream of US31W bridge at Munfordville UTMX: 598948 UTMY: 4125110 LATdd: 37.2692 LONGdd: -85.884 FIELD NUMBER: COLLECTORS: M.R. Thomas, J.G. Stewart, D. Zeman, B. Zeman, R.R. Cicerello COLLECTION DATE: 7 / 13 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Kentucky COUNTY: Hart MMNS TISSUE CATALOG NUMBER: 281.01 BASIN: Mississippi River DRAINAGE: Ohio SYSTEM: Green River WATER: Green River LOCALE: at first island upstream of US31W bridge at Munfordville UTMX: 598948 UTMY: 4125110 LATdd: 37.2692 LONGdd: -85.884 FIELD NUMBER: COLLECTORS: M.R. Thomas, J.G. Stewart, D. Zeman, B. Zeman, R.R. Cicerello COLLECTION DATE: 7 / 13 / 2004 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.14 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.01 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

49 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.15 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.13 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.12 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.11 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

50 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.1 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.09 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.02 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.08 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

51 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.03 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.04 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.05 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.06 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

52 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Oklahoma COUNTY: McCurtain MMNS TISSUE CATALOG NUMBER: 667.07 BASIN: Mississippi River DRAINAGE: Red SYSTEM: Little River WATER: Glover River LOCALE: Hwy 3 bridge crossing, W of North Pole UTMX: 324512 UTMY: 3774417 LATdd: 34.0976 LONGdd: -94.9023 FIELD NUMBER: MAD06-008 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 10 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47905.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: Osage MMNS TISSUE CATALOG NUMBER: 728.1 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Bird Creek LOCALE: at confluence with Candy Creek, along Hwy 11; E of Avant UTMX: 765517 UTMY: 4041380 LATdd: 36.4828 LONGdd: -96.0363 FIELD NUMBER: MAD06-022 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 13 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47512.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: Osage MMNS TISSUE CATALOG NUMBER: 728.09 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Bird Creek LOCALE: at confluence with Candy Creek, along Hwy 11; E of Avant UTMX: 765517 UTMY: 4041380 LATdd: 36.4828 LONGdd: -96.0363 FIELD NUMBER: MAD06-022 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 13 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47512.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: Osage MMNS TISSUE CATALOG NUMBER: 728.05 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Bird Creek LOCALE: at confluence with Candy Creek, along Hwy 11; E of Avant UTMX: 765517 UTMY: 4041380 LATdd: 36.4828 LONGdd: -96.0363 FIELD NUMBER: MAD06-022 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 13 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47512.0000 VOUCHER LOCATION: MMNS COMMENTS:

53 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Oklahoma COUNTY: Osage MMNS TISSUE CATALOG NUMBER: 728.07 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Bird Creek LOCALE: at confluence with Candy Creek, along Hwy 11; E of Avant UTMX: 765517 UTMY: 4041380 LATdd: 36.4828 LONGdd: -96.0363 FIELD NUMBER: MAD06-022 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 13 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47512.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: Osage MMNS TISSUE CATALOG NUMBER: 728.06 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Bird Creek LOCALE: at confluence with Candy Creek, along Hwy 11; E of Avant UTMX: 765517 UTMY: 4041380 LATdd: 36.4828 LONGdd: -96.0363 FIELD NUMBER: MAD06-022 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 13 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47512.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: Osage MMNS TISSUE CATALOG NUMBER: 728.01 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Bird Creek LOCALE: at confluence with Candy Creek, along Hwy 11; E of Avant UTMX: 765517 UTMY: 4041380 LATdd: 36.4828 LONGdd: -96.0363 FIELD NUMBER: MAD06-022 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 13 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47512.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: Osage MMNS TISSUE CATALOG NUMBER: 728.02 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Bird Creek LOCALE: at confluence with Candy Creek, along Hwy 11; E of Avant UTMX: 765517 UTMY: 4041380 LATdd: 36.4828 LONGdd: -96.0363 FIELD NUMBER: MAD06-022 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 13 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47512.0000 VOUCHER LOCATION: MMNS COMMENTS:

54 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Oklahoma COUNTY: Osage MMNS TISSUE CATALOG NUMBER: 728.03 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Bird Creek LOCALE: at confluence with Candy Creek, along Hwy 11; E of Avant UTMX: 765517 UTMY: 4041380 LATdd: 36.4828 LONGdd: -96.0363 FIELD NUMBER: MAD06-022 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 13 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47512.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: Osage MMNS TISSUE CATALOG NUMBER: 728.04 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Bird Creek LOCALE: at confluence with Candy Creek, along Hwy 11; E of Avant UTMX: 765517 UTMY: 4041380 LATdd: 36.4828 LONGdd: -96.0363 FIELD NUMBER: MAD06-022 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 13 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47512.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: Osage MMNS TISSUE CATALOG NUMBER: 728.08 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Verdigris River WATER: Bird Creek LOCALE: at confluence with Candy Creek, along Hwy 11; E of Avant UTMX: 765517 UTMY: 4041380 LATdd: 36.4828 LONGdd: -96.0363 FIELD NUMBER: MAD06-022 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 13 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 47512.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: Ottawa MMNS TISSUE CATALOG NUMBER: 695.03 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Neosho River WATER: Spring River LOCALE: Josephine Smith SP - bridge crossing UTMX: 344618 UTMY: 4088784 LATdd: LONGdd: FIELD NUMBER: MAD06-015 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 11 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: MMNS COMMENTS:

55 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: Oklahoma COUNTY: Ottawa MMNS TISSUE CATALOG NUMBER: 695.01 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Neosho River WATER: Spring River LOCALE: Josephine Smith SP - bridge crossing UTMX: 344618 UTMY: 4088784 LATdd: LONGdd: FIELD NUMBER: MAD06-015 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 11 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: Ottawa MMNS TISSUE CATALOG NUMBER: 695.04 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Neosho River WATER: Spring River LOCALE: Josephine Smith SP - bridge crossing UTMX: 344618 UTMY: 4088784 LATdd: LONGdd: FIELD NUMBER: MAD06-015 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 11 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: Ottawa MMNS TISSUE CATALOG NUMBER: 695.02 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Neosho River WATER: Spring River LOCALE: Josephine Smith SP - bridge crossing UTMX: 344618 UTMY: 4088784 LATdd: LONGdd: FIELD NUMBER: MAD06-015 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 11 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: MMNS COMMENTS:

STATE: Oklahoma COUNTY: Ottawa MMNS TISSUE CATALOG NUMBER: 695.05 BASIN: Mississippi River DRAINAGE: Arkansas SYSTEM: Neosho River WATER: Spring River LOCALE: Josephine Smith SP - bridge crossing UTMX: 344618 UTMY: 4088784 LATdd: LONGdd: FIELD NUMBER: MAD06-015 COLLECTORS: M.A. Dugo, W.T. Slack COLLECTION DATE: 4 / 11 / 2006 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: MMNS COMMENTS:

56 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.17 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.08 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.16 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.14 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

57 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.13 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV). Received

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.12 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.11 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.15 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

58 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.09 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.01 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.07 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.06 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

59 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.05 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.04 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.03 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.02 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

60 Appendix C. Collection Data for DNA Extractions, Percina copelandi.

STATE: West Virginia COUNTY: Gilmer MMNS TISSUE CATALOG NUMBER: 167.1 BASIN: Ohio River DRAINAGE: Little Kanawha SYSTEM: Little Kanawha River WATER: Little Kanawha River LOCALE: at the mouth of Copper Run in Gilmer UTMX: 524495 UTMY: 4303279 LATdd: 38.9799 LONGdd: -80.7176 FIELD NUMBER: JFS-05-02 COLLECTORS: J. Switzer, S. Welsh COLLECTION DATE: 6 / 14 / 2005 PRESERVATION: 100% ETOH DNA EXTRACTED: Yes MUSEUM VOUCHER REFERENCE: 0.0000 VOUCHER LOCATION: N/A COMMENTS: donated by John Switzer (USGS) and Stuart Welsh (UWV).

61