Anti-EPS15 (C-terminal) produced in rabbit, affinity isolated

Catalog Number SAB4200579

Product Description Antibody Concentration: ~1.0 mg/mL Anti-EPS15 (C-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to a Precautions and Disclaimer C-terminal sequence of human EPS15 isoform A This product is for R&D use only, not for drug, (GeneID: 2060), conjugated to KLH. The corresponding household, or other uses. Please consult the Material sequence is identical in human EPS15 isoform B, and Safety Data Sheet for information regarding hazards in rat and mouse EPS15. It is affinity-purified using the and safe handling practices. immunizing peptide immobilized on agarose. Storage/Stability Anti-EPS15 (C-terminal) specifically recognizes human, For continuous use, store at 2-8 °C for up to one rat and mouse EPS15. The antibody may be used in month. For extended storage, freeze in working several immunochemical techniques including aliquots. Repeated freezing and thawing, or storage in immunoblotting (~135 kDa), immunofluorescence and “frost-free” freezers, is not recommended. If slight immunohistochemistry. Detection of the EPS15 band by turbidity occurs upon prolonged storage, clarify the immunoblotting is specifically inhibited by the EPS15 solution by centrifugation before use. Working dilutions immunizing peptide. should be discarded if not used within 12 hours.

Eps15 (EGFR pathway substrate 15) is an endocytic Product Profile adaptor , that plays a central role in the clathrin- Immunoblotting: a working concentration of 1-2 mg/mL 1,2 mediated internalization and degradation of EGFR. is recommended using extracts of Jurkat, NIH3T3 and Eps15 contains three N-terminal conserved EH protein- PC12 cells. protein interaction domains, a central coiled-coil region, and a C-terminal domain containing the AP-2 and AP-1 Immunofluorescence: a working concentration of binding domains. The C-terminal domain also contains 5-10 mg/mL is recommended using HS68 cells. two ubiquitin interaction motifs (UIMs), required for Eps15 binding to ubiquitin. Activation of growth factor Immunohistochemistry: a working concentration of receptors induces tyrosine phosphorylation and mono- 10-20 mg/mL is recommended using formalin-fixed, ubiquitination of Eps15. Eps15 is required in clathrin- paraffin-embedded human prostate carcinoma. mediated endocytosis including of synaptic vesicles. Eps15 is a multifunctional adaptor protein and binds to Note: In order to obtain the best results using various AP-1 and AP-2 complexes and several other techniques and preparations, we recommend involved in endocytosis and/or synaptic vesicle determining the optimal working dilutions by titration. recycling, including Crk, Grb2, epsin, synaptojanin, Numb and parkin. Eps15 colocalizes with markers of References the plasma membrane clathrin-coated pits and 3 1. Fazioli, F., et al., Mol. Cell. Biol., 13, 5814-5828 vesicles. Eps15 has been shown to regulate the (1993). endosomal trafficking of EGFR, possibly through the 2. Zwang, Y., and Yarden, Y., Traffic, 7, 349-363 recruitment of the ubiquitinated receptors to the rims of 4 (2009). clathrin-coated pits. The EPS15 yields two 3. Torrisi, M.R., et al. Mol. Biol. Cell, 10, 417-434 isoforms that are believed to reside in distinct (1999). subcellular locations and thus implicated in different 5 4. Roxrud, I., et al., J. Cell Biol., 180, 1205-1218 aspects of endosomal trafficking. (2008). 5. Bernard, O.A.. et al., Oncogene, 9, 1039-1045 Reagent (1994). Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. ER,TD,PHC 04/13-1

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