Rabbit Anti-Merlin Rabbit Anti-Merlin

Qty: 100 μL Rabbit anti-Merlin Catalog No. 38-2201 Lot No. Rabbit anti-Merlin

FORM This polyclonal antibody is supplied as a 100 µL aliquot of neat antiserum containing 0.1% sodium azide.

PAD: ZMD.351

IMMUNOGEN Recombinant human Merlin isoform I protein

SPECIFICITY This antibody is specific for the ~69 kDa Merlin protein, also known as neurofibromatosis type 2 (NF2) or schwannomin. Cross reactivity with related proteins was not observed.

REACTIVITY Reactivity has been confirmed with human U251 glioma cell lysates, primary cultured rat Schwann cells, and Merlin- overexpressed NIH-3T3 cell lysates in both Western blot and immunoprecipitation assays.

Sample Western Immuno- Blotting precipitation Human +++ +++ Rat ++ ++ Mouse ++ ++ (Excellent +++, Good++, Poor +, No reactivity 0, Not applicable N/A, Not Determined ND)

USAGE Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Western Blotting: 1:1000 Immunoprecipitation: 1:50-1:100

STORAGE Store at 2-8°C for up to one month. Store at –20°C for long-term storage. Avoid repeated freezing and thawing.

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BACKGROUND The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes a protein product, merlin, or schwannomin, that is related to the ezrin-radixin-moesin (ERM) family of proteins. The two predominant merlin isoforms, I and II, differ only in the carboxy terminal 16 residues, and only isoform I is anti-proliferative.1 Merlin is involved with numerous signal transduction pathways that regulate cell growth and embryonic development. Mutation of merlin is associated with development of schwannoma tumors, and patients of NF2 are predisposed to other nervous system tumors such as ependymomas, meningiomas, and mesotheliomas.

Recent studies have shown that merlin increased p53 stability by inhibiting the Mdm2-mediated degradation of p53,2 and also directly inhibits the p21-activated kinase-1 (PAK1), which is essential for malignant growth of NF2-deficient cells.3

REFERENCES 1. Meng JJ, et al. J Neurosci Res 62(4): 491-502, 2000. 2. Kim H, et al. J Biol Chem 279(9): 7812-8, 2004. 3. Hirokawa Y, et al. Cancer J 10(1): 20-6, 2004.