JOURNAL OF VIROLOGY

VOLUME 12 * NUMBER 5 * NOVEMBER 1973

EDITORIAL BOARD

Robert R. Wagner, Editor-in-Chief (1977) University of Virginia School of Medicine, Charlottesville

Lloyd M. Kozloff, Editor (1974) Norman P. Salzman, Editor (1975) University of Colorado Medical Center National Institute of Allergy and Denver, Colo. Infectious Diseases, Bethesda, Md.

Myron Levine, Editor (1976) Aaron J. Shatkin, Editor (1977) School of Medicine, The Roche Institute of Molecular Biology, University of Michigan. Nutley, N. J. Ann Arbor

Dwight L. Anderson (1974) Angus Graham (1975) Bernard Moss (1974) David Baltimore (1975) D. MacDonald Green (1974) Elmer R. Pfefferkorn (1974) Marcel A. Baluda (1975) Klaus Hummeler (1973) Lennart Philipson (1973) Allan M. Campbell (1975) Paul J. Kaesberg (1973) Fred Rapp (1975) Purnell Choppin (1974) Albert S. Kaplan (1973) Bernard Roizman (1973) Eugene H. Cota-Robles (1973) Edwin D. Kilbourne (1973) Robert W. Simpson (1973) Vittorio Defendi (1973) David W. Kingsbury (1973) Donald F. Summers (1973) Walter Doerfler (1974) Lloyd M. Kozloff (1976) Howard M. Temin (1973) Richard M. Franklin (1973) Margaret Lieb (1973) Peter Vogt (1973) Robert M. Friedman (1973) Jean Lindenmann (1973) John S. Wiberg (1973) Harold S. Ginsberg (1973) Royce Z. Lockart, Jr. (1975) Julius Youngner (1973) Marc Girard (1974) Christopher K. Mathews (1973)

Robert A. Day, Managing Editor, 1913 I St. N.W., Washington, D.C. 20006

EX OFFICIO L. Leon Campbell, President (1973-1974) Philipp Gerhardt, Vice-President (1973-1974) Donald E. Shay, Secretary T. J. Carski, Treasurer

The Journal of Virolog, a publication of the American to the ASM Publications Office, 1913 I St., N.W., Wash- Society for Microbiology, 1913 I St., N.W., Washington, D.C. ington, D.C. 20006 (area 202 833-9416). Correspondence from 20006, is devoted to the dissemination of fundamental ASM members relating to membership dues, member knowledge concerning viruses of bacteria, plants, and ani- subscriptions. changes of address, incorrect address, mals. Investigators are invited to submit reports of original incorrect journals, etc., should be directed to the Executive research in all areas of basic virology. including biochemis- Secretary, American Society for Microbiology, 1913 I St., try, biophysics, genetics, immunology, morphology, and N.W., Washington, D.C. 20006. Published monthly by the physiology. The Journal is issued monthly, two volumes per ASM at 428 E. Preston St., Baltimore, Md. 21202. year. The subscription price is $60 (foreign, $61) per year; single copies are $6.00 (foreign, $6.25). Members of' the American Society for Microbiology may receive the Journal Second-class postage paid at Baltimore, Md. 21202, and as part of' their dues. Correspondence relating to sub- at additional mailing offices scriptions, reprints, defective copies, availability of' back Made in the U.S.A. issues, lost or late proofs, disposition of submitted manu- Copyright ( 1973, American Society for Microbiology. scripts, and general editorial matters should be directed All Rights Reserved. Author Index

Albu, Evelyn, 1164 Greenman, Richard L., 995 Okabe, Hiromi, 984 Allen, Patton T., 1049, 1085 Guttman, Naomi, 1181 Anderson, Dwight L., 1149 Pagano, Joseph S., 1006 Anderson, Gerry C., 1191 Hagedorn, C., 1031 Papas, T. S., 1184 Aposhian, H. Vasken, 1065 Hagen, Elaine W., 1149 Pattee, P. A., 1031 Aron, Gary M., 1043, 1118 Hastie, N. D., 951 Peters, Elizabeth D., 1104 Hatanaka, Masakazu, 984 Pootjes, Christine F., 1043,1118 Bachi, Thomas, 1173 Hawley, Louise A., 1149 Purcell, Robert H., 995 Baltimore, David, 1122,1181 Hirumi, H., 1184 Beckman, L. David, 1191 Holmes, Kathryn V., 1164 Reilly, Bernard E., 1149 Berryhill, D. L., 1031 Holt, J. G., 1031 Repik, Patricia, 969 Bishop, D. H. L., 969 Howe, Calderon, 1173 Robb, James A., 1187 Bradley, David E., 1139 Hummeler, Klaus, 1028 Robinson, William S., 995 Jansz, H. S., 1131 Russell, P. K. A., 1034 Chirigos, M. A., 1184 Johnson, K., 1160 Chow, C. T., 1160 Schlesinger, Harvey R., 1028 Kaplan, Paul M., 995 Summers, Max D., 1092 Dalrymple, J. M., 1034 Kawai, Yasuo, 1006 Sussenbach, J. S., 1131 Dmochowski, Leon, 1049,1085 Kerr, Sylvia J., 1013 Drexler, Henry, 1072 Kingsbury, D. W., 1020 Knesek, John E., 1049, 1085 Teramoto, A. Y., 1034 East, James L., 1049,1085 Egawa, Kohji, 1092 Lyric, R. M., 1160 Van Caeseele, L., 1160 Einck, K. H., 1031 Vogel, S. N., 1034 Ellens, D. J., 1131 McCorquodale, D. J., 1191 Mahy, B. W. J., 951 Wells, Henry J., 1028 Fernandez-Tomas, Carlos B., Marmur, Julius, 1078 Wilkinson, Ray, 1013 1122, 1181 Miller, J. A., 1031 Woods, W. A., 1184 Neubort, Simon, 1078 Gerin, John L., 995 Nonoyama, Meihan, 1006 Yeh, Jen, 962 Gilden, Raymond V., 984 Nowinski, Robert C., 1104 Yelton, David B., 1065 JOURNAL OF VIROLOGY INSTRUCTIONS TO AUTHORS Correspondence. Submit manuscripts in dupli- of each paper in the Journal. The abstract should cate (original and one carbon) to ASM Publica- not exceed 250 words. tions office, 1913 I St., N.W., Washington, D.C. Literature Cited. In the text references are cited 20006. by number. The Literature dited section should General policy. Any manuscript submitted must be typed in alphabetical order, by first author, and be a report of unpublished original research, which numbered. Names of journals are abbreviated ac- is not being considered for publication elsewhere. cording to Chemical Abstracts Service Source In- A manuscript accepted and published by the dex (American Chemical Society, 1970). Litera- Journal must not be published again in any form ture citations should be restricted to closely without the consent of ASM. pertinent papers. Citations of abstracts, theses, A charge of $25 per printed page is assessed for "unpublished data," "personal communication, " publication in the Journal. Most institutions and and "in press" will not be accepted in the Litera- granting agencies in the United States permit the ture Cited, but may be used parenthetically in payment of publication charges as part of their the text. general research support. It is recognized, how- Tables. Each table should be typed on a sepa- ever, that such funds are not always available to rate page. The data should be arranged so that laboratories outside the United States. In such columns of like material read down, not across. cases, the cost of publication will be borne by the The headings should be sufficiently clear so that American Society for Microbiology. Ability or in- the meaning of the data will be understandable ability to pay the publication charge is not a factor without reference to the text. Explanatory foot- in the selection of manuscripts for publication. notes are permitted, but detailed descriptions of The "editorial style" of the Journal essentially the experiments are not. The materials and meth- follows the CBE Style Manual (3rd ed., AIBS, ods used to gain the data should properly remain 1972). Biochemical abbreviations and nomen- in the section of that name. clature should essentially follow "Biochemical Figures. A complete set of figures, preferably Nomenclature" in Handbook of Biochemistry, glossy photographs, should accompany each of the (2nd ed., 1970, H. A. Sober, ed., The Chemical two copies of the manuscript. Each figure should Rubber Co., Cleveland, p. A4-A24). Normally, be numbered and should include the name of the abbreviations (except those of standard units of author, either in the margin or on the back (marked measurement and symbols of the elements) should lightly with a soft pencil). Graphs (submit as be defined and introduced parenthetically at first photographs) should be finished drawings not use in the text. Certain abbreviations will be ac- needing further artwork or type-setting. Ab- cepted without definition in the title, abstract, and solutely no part of a graph should be typewritten text. Among these abbreviations are: DNA (de- (except the legend, which should be typed on a oxyribonucleic acid); RNA (ribonucleic acid); separate page). All lettering should be done with rRNA (ribosomal RNA); mRNA (messenger a lettering set. Most graphs will be reduced to one- RNA); tRNA (transfer RNA); AMP, ADP, ATP, column width, and all elements in the drawing dAMP, dADP, dATP (for the respective 5' phos- should be prepared to withstand this reduction. phates of adenosine or the other nucleosides, using The legend of the figure should provide enough appropriate letter symbols); 2'-AMP, 3'-AMP, information so that the figure is understandable 5'-AMP (the 2'-, 3'-, and 5'-, where needed for con- without reference to the text. Experimental de- trast, phosphates of the nucleosides); DNase tails from Materials and Methods should not be (deoxyribonuclease); RNase (ribonuclease); P i repeated in figure legends. (orthophosphate); PPi (pyrophosphate); mol wt Notes. The accepted form for Notes is somewhat (molecular weight); UV (ultraviolet); PFU different from the foregoing. Contributors should (plaque-forming units); Tris (tris (hydroxy- consult a recent issue of the Journal for style. methyl)aminomethane); DEAE- (diethylamino- Notes should not exceed 500 words. The Abstract ethyl-); and EDTA (ethylenediaminetetraace- should not exceed 25 words. Papers submitted as tate). Genetic symbols should essentially follow Notes which are fragments of full-length papers the recommendations of Demerec et al. (Genetics are not acceptable. Consideration will be given 54:61, 1966). Enzyme activity should be expressed to publication of Notes which consist of com- in terms of international units (Enzyme Nomen- mentaries or interpretations of principles of vir- clature, Elsevier Publishing Co., 1965), and the EC ology. number should be given parenthetically at first use Nomenclature of viruses. Viruses named after a in the text. In expressing lengths, weights, and disease should be written as separate words in volumes, the prefixes nano (n) and pico (p) should lower-case roman, except when the name of the be used instead of millimicro (mM) and micromicro disease is derived from a proper noun: herpes (,uA). Express lengths in nanometers (nm; 10-9 m) simplex virus, measles virus, Newcastle disease or in micrometers (pLm; 10-6 m) instead of milli- virus, poliomyelitis virus, varicella-zoster virus, microns (m,u; 10-9 m), microns (Iu; 10-6 m), or vaccinia virus. Angstroms (A; 10-0im). Express parts per million Most other viruses should be written as a single (ppm) as micrograms per milliliter (jug/ml), micro- word in lower case: adenovirus, coxsackievirus, grams per gram (,ug/g), or microliters per liter cytomegalovirus, echovirus, herpesvirus, mengo- (juliters/liter), as appropriate. In general, measure- virus, picornavirus, poliovirus, poxvirus. ments should be expressed in terms of standard Copyright. Once a paper has been published in international metric units. The Journal reserves the Journal, which is a copyrighted publication, the privilege of editing manuscripts to make them the legal ownership of all parts of the paper, in- conform with the adopted style. cluding the illustrations, has passed from the Form of manuscript. All parts of the manuscript author to the Journal. If the same author, or any should be typed double-space or, preferably, author, wishes to republish material previously triple-space. Most manuscripts can and should be published in the Journal, he must first receive divided into the following sections: Abstract, In- written permission from ASM. troduction, Materials and Methods, Results, Dis- Reprints. Reprints (in multiples of 100) will be cussion, Acknowledgments, and Literature Cited. Only one weight of heading (paragraph lead-in) furnished contributors when ordered in advance. should be used within each section. A table showing the cost of reprints, and an order Abstract. An Abstract appears at the beginning form, will be sent with the proof. The 1974 ICN/UCLA Winter Conferences on Molecular Biology MECHANISMS OF VIRUS DISEASE To be held at Squaw Valley, California March 25-29, 1974 William S. Robinson and Peter H. Duesberg, Chairmen IMMUNOLOGICAL INJURY IN VIRUS DISEASE- WHERE IS THE VIRUS OF HUMAN HEPATITIS?- Chairman: Thomas Merigan, Stanford: "Beneficial and Chairman: William Robinson, Stanford: "A DNA polym- adverse effects of viral immunity." erase in hepatitis B antigen." Paul Black, Harvard: "Lymphocyte mediated immuno- John Gerin, Oak Ridge National Lab: "Structure of pathology." hepatitis B antigen." Micheal Oldstone, Scripps: "Lymphocytic choriomenin- Robert Purcell, NIH: Infectivity of hepatitis B virus." gitis virus disease." Thomas Edgington, Scripps: "Immunology of hepatitis Abner Notkins, NIH: "Cellular immunity to herpes B." simplex." MECHANISM OF CELL TRANSFORMATION BY DNA Neal Nathanson, Johns Hopkins: "Immunopathology TUMOR VIRUSES-Chairman: Walter Eckhart, Salk in LCM infections." Institute: "Polyoma gene function." INFLUENCE OF HOST GENETIC FACTORS ON Robert Pollack, Cold Spring Harbor: "Phenotypic char- VIRUS DISEASE-Chairman: Frank Lilly, Einstein, acteristics of transformed cells." N.Y.: "Genetics of murine leukemia virus susceptibil- Malcom Martin, NIH: "Viral specific RNA in trans- ity." formed cells." Natalie Teich, NIH: "Genetic control of endogenous CELL TRANSFORMATION BY RNA TUMOR VIRUSES murine leukemia virus." -Chairman: Hideseburo Hanafusa, Rockefeller: "Tem- Richard Steeves, Einstein, N.Y.: "Friend leukemia perature sensitive mutants of Rous sarcoma virus." virus." Peter Fischinger, NIH: "Mouse sarcoma virus trans- formed cell revertants." DEFECTIVE VIRUSES: THEIR FORMATION AND Daniel Rifkin, Rockefeller: "Cell surface changes." INFLUENCE ON THE COURSE OF INFECTION- Peter Duesberg, Berkeley: "Transformation and viral Chairman: Alice Huang, Harvard: "Vesicular Stomatitis RNA structure." virus: defectiveness and disease." CONTROL MECHANISMS FOR PHAGE-Chairman: M. E. Reichmann, Illinois: "Temperature sensitive Mark Ptashne, Harvard: "Phage lambda." mutants of VSV." A. L. Taylor, Colorado: "Phage Mu." David Baltimore, MIT: "Polio virus." Richard Calender, Berkeley: "Phage P-2." John Holland, San Diego: "Interfering particles in David Botstein, MIT: "Phage P-22." vivo." ONCOGENIC HERPES VIRUSES-Chairman: George VIRUSES AND CHRONIC CENTRAL NERVOUS Miller, Yale: "Oncogenicity of Epstein-Barr virus." SYSTEM DISEASE-Chairman: Bernard Fields, Al- Bernard Roizman, Chicago: "Structure of herpes. bert Einstein, N.Y.: "Viral mutants and CNS infection." viruses." Hilary Koprowski, Wistar Institute: "Rabies and parain- Alan Granoff, St. Judes: "Luck6 tumor virus." fluenza viruses." Fred Rapp, Pennsylvania State: "Transformation by Richard Johnson, Johns Hopkins: "SV40 and progres- herpes simplex and cytomegalo virus." sive multifocal leukoencephalopathy." Neihan Nonoyama, Rush-Presbyterian, Chicago: "Hy- Fred Rapp, Pennsylvania State: "Measles virus." bridization with Epstein-Barr virus DNA." ATTENDANCE AT THE CONFERENCE IS BY INVITATION, AND INVITEES WILL BE SELECTED FROM APPLICATIONS RECEIVED AT THE CONFERENCE OFFICE NO LATER THAN JANUARY 15, 1974. The costs for attending the conference are a conference fee of $50 and the cost for lodging and meals, $75 for economy accommodations (double occupancy with "dormitory style" bath- room facilities) and $110 for deluxe accommodations (double occupancy with private bath). These prices include breakfast, the evening meal, coffee breaks, orientation party, a daily cock- tail hour, etc. The facilities at Squaw Valley can accommodate the wives or husbands and families of the conferees. The deluxe accommodations, however, are limited in availability and will be assigned on a first come, first serve basis. REQUEST FOR APPLICATION FORMS MUST BE RECEIVED AT THE CONFERENCE OFFICE NO LATER THAN DECEM- BER 15. NO MONEY SHOULD BE MAILED WITH REQUESTS FOR APPLICATION FORMS. Conference Office: Virus Research c/o Department of Bacteriology University of California Los Angeles, California 90024