PNMA2 Monoclonal Antibody (M10), Clone 4F10

PNMA2 monoclonal antibody (M10), clone 4F10

Catalog # : H00010687-M10 規格 : [ 100 ug ] List All

Specification Application Image

Product Mouse monoclonal antibody raised against a full-length recombinant Western Blot (Transfected lysate) Description: PNMA2.

Immunogen: PNMA2 (AAH62301, 1 a.a. ~ 364 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence: MALALLEDWCRIMSVDEQKSLMVTGIPADFEEAEIQEVLQETLKSLGRYR LLGKIFRKQENANAVLLELLEDTDVSAIPSEVQGKGGVWKVIFKTPNQDT EFLERLNLFLEKEGQTVSGMFRALGQEGVSPATVPCISPELLAHLLGQA enlarge MAHAPQPLLPMRYRKLRVFSGSAVPAPEEESFEVWLEQATEIVKEWPVT Western Blot (Recombinant EAEKKRWLAESLRGPALDLMHIVQADNPSISVEECLEAFKQVFGSLESRR protein) TAQVRYLKTYQEEGEKVSAYVLRLETLLRRAVEKRAIPRRIADQVRLEQV MAGATLNQMLWCRLRELKDQGPPPSFLELMKVIREEEEEEASFENESIE Immunofluorescence EPEERDGYGRWNHEGDD

Host: Mouse

Reactivity: Human

Isotype: IgG2a Kappa enlarge

Quality Control Antibody Reactive Against Recombinant Protein. Immunoprecipitation Testing:

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Sandwich ELISA (Recombinant protein)

Western Blot detection against Immunogen (65.78 KDa) .

In 1x PBS, pH 7.4 Storage Buffer: enlarge

Storage Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. ELISA Instruction:

MSDS: Download

Datasheet: Download

Applications Western Blot (Transfected lysate)

Page 1 of 3 2016/5/23 Western Blot analysis of PNMA2 expression in transfected 293T cell line by PNMA2 monoclonal antibody (M10), clone 4F10.

Lane 1: PNMA2 transfected lysate (Predicted MW: 41.5 KDa). Lane 2: Non-transfected lysate. Protocol Download

Western Blot (Recombinant protein) Protocol Download

Immunofluorescence

enlarge this image Immunofluorescence of monoclonal antibody to PNMA2 on HeLa cell . [antibody concentration 10 ug/ml] Protocol Download

Immunoprecipitation

Immunoprecipitation of PNMA2 transfected lysate using anti-PNMA2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PNMA2 monoclonal antibody. Protocol Download

Sandwich ELISA (Recombinant protein)

Page 2 of 3 2016/5/23 Detection limit for recombinant GST tagged PNMA2 is approximately 3ng/ml as a capture antibody. Protocol Download

ELISA

Gene Information

Entrez GeneID: 10687

GeneBank BC062301 Accession#:

Protein AAH62301 Accession#:

Gene Name: PNMA2

Gene Alias: KIAA0883,MA2,MM2,RGAG2

Gene paraneoplastic antigen MA2 Description:

Omim ID: 603970

Gene Ontology: Hyperlink

Other onconeuronal antigen MA2,paraneoplastic neuronal Designations: antigen,retrotransposon gag domain containing 2

Page 3 of 3 2016/5/23