RESEARCH ARTICLE A revised classification of Chinese Davalliaceae based on new evidence from molecular phylogenetics and morphological characteristics 1,2 3 1 4 1 Xiao-Dong MaID , Ai-Hua Wang , Fa-Guo WangID *, Chun-Mei He , Dong-Ming Liu , Pedro Gerstberger5, Fu-Wu Xing1 a1111111111 1 Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China, 2 Chinese Academy of Forestry Research Institute a1111111111 of Forestry, Beijing, China, 3 Key Laboratory of Environment Change and Resources Use in Beibu Gulf a1111111111 (Guangxi Teachers Education University), Ministry of Education, Nanning, Guangxi, China, 4 Guangdong a1111111111 Academy of Forestry, Guangzhou, Guangdong, China, 5 Department of Plant Ecology, University of a1111111111 Bayreuth, Bayreuth, Bavaria, Germany *
[email protected] OPEN ACCESS Abstract Citation: Ma X-D, Wang A-H, Wang F-G, He C-M, Liu D-M, Gerstberger P, et al. (2018) A revised Although the phylogenetic framework of Davalliaceae is known, the classification of Chinese classification of Chinese Davalliaceae based on new Davalliaceae is still controversial. In this study, a molecular phylogenetic tree of 60 acces- evidence from molecular phylogenetics and sions, including 29 species produced in China, was constructed using five plastid DNA morphological characteristics. PLoS ONE 13(11): markersÐatpB, atpB-rbcL, rbcL, rbcL-accD, and accD. New data on studied specimens, e0206345. https://doi.org/10.1371/journal. pone.0206345 field investigations, and scanning electron microscopy analysis of leaf epidermis and spores were used to reclassify Chinese Davalliaceae. The taxonomic position of Davallia canarien- Editor: Zhong-Jian Liu, The National Orchid Conservation Center of China; The Orchid sis was confirmed based on new evidence and a new key to sections of Chinese Davallia- Conservation & Research Center of Shenzhen, ceae was proposed.