Acta Zootaxonomica Sinica, 32 ( 4) : 792- 801 ( Oct. , 2007) ISSN 1000 0739 动物分类学报
A NEW SPECIES OF RANA ( ANURA, RANIDAE) FROM CHINA 1 1* 2 LU Yu Yan , LI Pi Peng , JIANG De Bin 1. Liaoning K ey Laboratory of Evolution and Biodiversity , Shenyang Normal University , Shenyang 110034, China 2. Management Bureau of Maoershan National Nature Reserve , Guilin 541316, China
Abstract A new species of Rana is described from Guangxi Province, China. The new species, Rana maoershan ensis has a larger body length, with a SVL averaging 50 9 mm for males and 54 6 mm for females. Other characters in combination that separate it from close relatives include head width broader than head length; dorsalateral fold thickened and slightly curved; skin rather smooth; tibio tarsal joint reaching anterior corner of eye; outer metatarsal tubercle indistinct; no internal vocal sacs, nuptial pad divided into two groups, and lineae musculinae absent ventrally in male; and labial tooth row formula of tadpole : 3 3 : 1 1 more or : 3 3 ! less. Key words Anura, Ranidae, Rana maoershanensis , new species, China.
The Eurasian brown frogs are morphological reared through metamorphosis to confirm the conservative assemblage consisting a larger number of identification and to describe the coloration and compare similar species ( Liu, 1946; Liu & Hu, 1961; Kim et with adult patterns. They were fed with egg yolk and al . , 2002) . In some species group, the frogs are quite vegetable leaves in captivity in a plastic box ( 260 mm ∀ similar in morphology, and very difficulty to identify 175 mm ∀ 160 mm) with 1 5 liters of water. ( Liu & Hu, 1961; Tanaka et al . , 1996; Xie et al . , Measurements were made with digital calipers to the 2000; Lu & Li, 2002) . During last decade, more than 5 nearest 0 01 mm and rounded to the nearest 0 1 mm. new species were described from China and 13 species External measurements ( Fei et al . , 2005 ) included have been known distributed in China ( Fei et al . , 2005; snout vent length ( SVL, from tip of snout to vent) ; Li et al . , 2005; Lu & Li, 2005) . The taxonomic status head length ( HL, from tip of snout to hind border of and phylogenetics of some species, such as Rana angle of jaw) ; head width ( HW, width of head at its kunyuensis, R . zhenhaiensis, R. omeimontis , were made widest point ) ; internarial distance ( IND, distance clearly by molecular analysis based on DNA sequence between nares) ; interorbital distance ( IOD, minimum ( Che, 2006; Jiang & Zhou, 2001; Yang et al . , 2001) . distance between upper eyelids) ; snout length ( SL, from This indicates that complexes of cryptic species maybe anterior border of eye to tip of snout) ; diameter of eye exist in different species group of brown frogs in China ( ED, horizontal diameter of eye) ; width of upper eyelid ( Lu & Li, 2005) . ( UEW, maximum width of upper eyelid) ; diameter of Recent herpetological surveys in China have resulted tympanum ( TD, horizontal diameter of tympanum) ; in the discovery of brown frogs similar to Rana forelimb length ( FLL, from elbow to tip of third finger) ; chaochiaoensis in Guangxi, China ( Li et al . , 2006) . After hand length ( HLT, from base of outer palmer tubercle to collecting more adults and tadpole specimens and tip of third finger ) ; diameter of lower arm ( LAD, comparing with other brown frogs from China, it clearly maximum diameter of lower arm ) ; hindlimb length shows that the frog is a previously undescribed species ( HLL, from vent to tip of fourth toe) , tibia length and report here. ( TIL, from knee to foot) ; tarsus and foot length ( TFL, 1 Materials and Methods from tibio tarsal joint to tip of fourth toe) and foot length ( FL, from proximal end of metatarsal tubercle to tip of , Field surveys were conducted in February March fourth toe) . 2006 and July in Maoershan National Nature Reserve at All tadpoles were staged according to Gosner , , . Xinan County Guangxi Province China Latitude and ( 1960 ) . Tadpoles at stages 36 were used in the longitude were recorded with a Garmin eTrex Venture descriptions and measured. The measurements, . 10% GPS Specimens were fixed in buffered formalin terminology, and labial tooth row formula follow 70% , before preserved in ethanol and deposited in Mcdiarmid and Altig ( 1999) and the labial tooth row Shenyang Normal University Museum of Natural History formula is shown according to Liu and Hu ( 1961) . (SYNU) . Some tadpoles ( series SYNU 0602002) were All measurements were made with a digital caliper to
The research was funded by National Natural Science Foundation of China ( No. 0470206) and set up grand of invited professorship of Shenyang Normal University to Dr. LI P P. * Corresponding author, E mail: lipipeng@ yahoo. com Received 21 May 2007, accepted 10 July 2007. 792 Oct. , 2007 陆宇燕等: 中国蛙类一新种 ( 无尾目, 蛙科) 793 the nearest 0 01 mm and rounded to the nearest 0 1 mm. for these frogs also were taken from the original The tadpoles were observed and measured in a descriptions and references ( Liu, 1945, 1946; Ye et al . , stereomicroscope, except for total length, which was 1993; Ye et al . , 1995; Xie et al . , 2000) . measured with the caliper directly. The pictures were 2 Description of the New Species photographed with digital camera Nikon D100. In addition to the new species presented here, other Rana maoershanensis sp. nov. ( Figs 1 17, Tables 1 2) brown frogs of China ( Appendix apecimens examined) Holotype adult male, SYNU 06020120, China, were investigated with the specimens stored at SYNU, Guangxi Province, Xian County, Maoershan National Kunming Institute of Zoology ( KIZ) and Chengdu Natural Reserve, collected on the Pond in wetland Institute of Biology ( CIB) , of which R. chensinensis , R . ( 25#52∃N, 110#24∃E) , 1 980 m elevation, collected by kunyuensis , R. huanrenensis, R . chaochiaoensis , R . LI Pi Peng and WANG Shao Neng, Feb. 2006, and omeimontis and R. zhenhaiensis collected by the second Stored at SYNU. author from their type locality from 2000 to 2006. Data Table 1. Measurements ( in mm) of Rana maoershanensis sp. nov. Paratypes Paratypes Paratypes Paratypes Characters Holotype Characters Holotype Male (n = 3) Female (n = 4) Male (n = 3) Female ( n = 4) SVL 51 2 44 9 54 4* 52 1 57 6 TD 4 0 3 4 3 8 3 7 4 1 49 7 % 3 18* * 54 6 % 2 32 7 9% 3 6 % 0 12 3 9 % 0 19 7 3% 7 1%
HL 13 8 13 4 14 5 18 8 19 2 FLL 22 8 20 7 23 1 24 5 25 7 27 0% * * * 13 9 % 0 40 17 7 % 1 30 44 4% 21 9 % 0 81 25 6 % 1 02 27 9% 32 4% 44 1% 47 0% HW 17 1 15 2 17 6 17 9 20 7 LAD 6 8 6 5 7 1 4 5 5 3 33 4% 16 8 % 1 03 19 0 % 1 17 13 3% 6 8 % 0 22 4 9 % 0 41 33 7% 34 9% 13 6% 8 9%
SL 6 7 6 3 6 8 7 1 9 0 HLL 92 8 81 1 90 6 95 3 101 7 13 1% 6 6 % 0 16 7 6 % 0 41 181 3% 86 4 % 3 12 98 3 % 2 26 13 2% 14 0% 173 9% 180 1%
IND 5 1 4 5 5 5 5 4 6 1 TIL 29 1 26 2 28 8 30 4 31 6 10 0% 5 1 % 0 43 5 8 % 0 34 56 9% 27 6 % 0 96 30 9 % 0 58 10 3% 10 5% 55 5% 56 6%
IOD 3 4 3 1 3 3 3 1 4 5 TFL 43 0 37 7 43 2 43 2 48 1 6 7% 3 2 % 0 12 3 9 % 0 71 84 0% 40 3 % 1 93 45 7 % 1 89 6 5% 7 1% 81 0% 83 8%
UEW 3 9 4 2 4 6 3 5 4 9 FL 29 5 25 5 29 2 28 9 32 2 7 6% 4 4 % 0 19 4 2 % 0 62 57 6% 27 7 % 1 47 30 9 % 1 40 8 9% 7 7% 55 7% 56 7%
ED 7 0 6 1 6 7 6 14 6 42 TD: ED 0 60 0 55 0 61 6 5 % 0 22 6 30 % 0 09 IOD: TD 0 85 0 99 1 00 13 6% 13 0% 11 54% ED: SL 1 00 0 90 0 83
Note: * size range, * * mean % SD, * * * % SVL. Paratypes. Adult males, SYNU 06020091, SYNU thickened and slightly curved; skin rather smooth; tibio 06020122 and SYNU 06020124 ( males) ; and adult tarsal joint reaching anterior corner of eye; outer females SYNU 06020121, SYNU 06040153, SYN metatarsal tubercle indistinct; no internal vocal sacs, 0604154 and SYNU 06040155 ( females) , same data as nuptial pad divided into two groups, and lineae for holotype, collected by LI Pi Peng and WANG Shao musculinae absent ventrally in male; and labial tooth row Neng, Feb. and Apr. 2006 and stored at SYNU. formula of tadpole &3 3 &1 1 more or &3 3 ! Tadpoles. Series SYNU 0602001 and series SYNU less. 0604001, same data as for paratypes and stored at Eytmology. This new species is named after its type SUNU. locality. Diagnosis. This new species, Rana maoershanensis has Description of holotype ( Figs 1, 5 9 and Table 1) a larger body length, with a SVL averaging 50 9 mm for Habitus medium slender and larger; head broad, shorter males and 54 6 mm for females. Other characters in than wide; snout obtusely pointed in dorsal view, combination that separate it from close relatives include projecting beyond lower jaw, round in profile, slightly head width broader than head length; dorsalateral fold depressed from level of nostril to tip of snout; nostril 7 94 Acta Zootaxonomica Sinica 动物分类学报 Vol. 32 No. 4
Figs 1 9. Rana maoershanensis sp. nov. 1. Holotype ( male, SYNU 06020120) . 2. Paratype ( female, SYNU06040155) . 3 4. Paratype ( male, SYNU06020124) . 5. Dorsal view. 6. Ventral view. 7. Lateral view of head. 8. Palmar view of hand. 9. Tarsal view of Foot. Scale bars: 5 6, 8 9= 10 mm, 7= 5 mm. slightly closer to tip of snout than eye; canthus distinct, eye diameter 104% the snout length, and separated from slightly constricted at nostrils; lores concave and oblique; eye by less than the half of tympanic diameter; internasal Oct. , 2007 陆宇燕等: 中国蛙类一新种 ( 无尾目, 蛙科) 795
Figs 10 13. Paratypes of Rana maoershanensis sp. nov. 10. Dorsal view of the males ( SYNU 06020091, SYNU 06020122 and SYNU 06020124) . 11. Ventral view of the males ( SYNU 06020091, SYNU 06020122 and SYNU 06020124) . 12. Dorsal view of the females ( SYNU 06020121, SYNU 06040153 and SYN 0604154) . 13. Ventral view of the females ( SYNU06020121, SYNU 06040153 and SYN 0604154) . distance wider than interorbital distance and upper eyelid width; interorbital distance less than width of upper 7 96 Acta Zootaxonomica Sinica 动物分类学报 Vol. 32 No. 4
Figs 14 17. Tadpoles of Rana maoershanensis sp. nov. at stage 36 from the series SYNU 0602001. 14. Dorsal view. 15. Ventral view. 16. Lateral view. 17. Oral disc. eyelid; distinct, round tympanum 60% the eye surface of finger to the level of the distal edge of the diameter, not depressed relative to skin of temporal subarticular tubercle, small one extending from the region; vomerine teeth on two oblique ridges, about anterior of the big one onto anterior of figure . equal in distance from each other as to choanae; tongue Hindlimb long; tibio tarsal joint reaching anterior deeply notched posteriorly, free for approximately two corner of eye; heels only touching when legs are held at thirds its length; no internal vocal sacs. right angles to body; tips of all toes similar to figures; Forearm much more robust in males, tips of fingers relative toe lengths < ∋ < (< < !; toes fully no expanded and obtuse, without circummarginal webbed; subarticular tubereles well developed; inner grooves; relative finger lengths ∋ < ! < < ; metatarsal tubercle large, elongate, and oval; outer subarticular tubercles prominent, one subarticular tubercle metatarsal tubercle indistinct. on fingers and ∋, two subarticular tubercles on Skin rather smooth; thickened dorsolateral fold fingers and !; supernumerary tubercle at base of all slightly curved from temporal fold near rear of eye to four fingers weak; two metacarpal ( palmar) tubercles sacrum; elongate rictal gland above insertion of forearm; small, inner bigger andovoid, outer smaller and temporal fold distinctly extending from above tympanum elongate; velvety nuptial pad material covering granular curving to posterior, a large triangular gray patch behind structure, divided into two groups, big one ventricular, the eye and anterior to temporal fold; ventral surface of extending from base of palmar onto dorsal and medial body smooth except for the posterior, median aspects of Oct. , 2007 陆宇燕等: 中国蛙类一新种 ( 无尾目, 蛙科) 797 the femurs with coarsely whitish granular glands. Lineae nostril slightly closer to the tips of snout; spiraculum musculinae distinct dorsally, especially in the anterior small, on left side of body, no free tube; vent dextral, part, but absent ventrally. tube of vent continuous with ventral caudal fin; dorsal fin Coloration of holotype in life ( in preservative ) . rising from the base of tail. Tail height about third fifths (Figs 1, 5 9) . Dorsum even rufous or brown ( gray) , body length, the end part rounded obtusely; musculation with dark brown bars and blotchy present; narrow black developed with pointed tip; the mouth anteroventral, a stripe on edge of canthus from tip of snout along margin row of labial papillae on the corner of mouth and the of upper eyelid and across eye continuing below edge of lower lip, papillae of lower lip regularly arranged; few dorsolateral fold fades to gray; anterior of upper lip stripe additional papillae on the corner of mouth and short row dark brown, medium and posterior of upper lip knurled of papillae inside the end of the lower lip row; horny above dark brown stripe with reddish ( gray ) color beak weak and narrow. Labial tooth row formula extending to arm insertion; lower lip deeply brown ( LTRF ) : : 3 3 : 1 1 57 7% , : 3 3 ! stippled with whitish mass; rictal glands reddish ( pale) ; 26 9% , : 3 2 : 1 1 7 7%, 4 4 : 1 1 and : triangular patch gray cyan with black spots ( gray with 2 2 : 1 1 3 8% respectively in 26 samples of the series dark spots) ; dorsum of arm pale brown and forearm SYNU 0602001 and series SYNU 0604001 at stage from brown with dark brown cross bars and flecks ( gray with 33 to 38. dark cross bars and spots) ; dorsum limbs brown with Coloration of tadpole ( Figs 13 16) . In life generally dark cross bar at thigh and tibia respectively ( brown with olivaceous with dark, small dots at dorsal view of body dark cross bars ) ; dorsalateral fold reddish brown and tail; lightly olivaceous covered with creamish ( yellow) ; upper portion of flank brown with dark spots sparkling spots at lateral view of body and musculation; ( gray with dark spots) ; lower half of flank creamy fins translucent pale gray with brown marbled; creamish yellow ( white) , with brown mottling; axial and inguinal sparkle at ventral view of body. In preservative, body areas immaculate yellow ( white ) ; white mottling on darker than tail; belly lighter than dorsum and gut underside of outer margins of lower jaw; throat and belly visible; tail lightly gray with dark marbled. creamish with gray nebulous mottled ( white with pale Table 2. Mean measurements and standard deviation (in mm) gray nebulous mottled) ; ventral surface of thigh mostly of six Rana maoershanensis tadpoles of the series 0602001 at stage immaculate fleshcolor with yellow mobbling near inguinal 36. area and posterior yellow ( white) ; foot webbing brown Characters Size rang Mean% SD % BL ( brown) ; nuptial pad gray ( dark) . Variation of paratypes. Measurements of paratypes Total length 51 4 58 5 54 2 % 2 38 279 4% summarized in Table 1. Snout vent length ( SVL) 44 9 Body length ( BL) 19 1 20 1 19 4 % 0 43 Bogy width ( BW) 12 3 15 3 13 6 % 1 06 70 1% 54 4 mm ( mean 49 7 mm) in adult males and 52 1 57 6 Body height ( BH) 10 3 13 2 11 6 % 1 02 59 8% mm ( mean 54 6 mm) in adult females, eye diameter Tail length 31 3 39 0 34 8 % 2 51 179 4% 78% 88% ( mean 83% ) the snout length in females and Tail depth 11 6 12 8 12 0 % 0 59 61 9% 97% 100% ( 99% ) , 59% mean in males tympanum Upper fin height 3 2 3 9 3 5 % 0 33 18 0% 64% ( mean 61%) the eye diameter in females and 52% Lower fin height 2 0 2 8 2 5 % 0 27 12 9% 59% ( mean 55%) in males, Hind limb 180% snout Eye diameter 2 0 2 1 2 0 % 0 05 10 3% vent length in females and 173% in females. Forearm Snout eye distance 5 2 6 0 5 7 % 0 3 29 4% much more robust in males than in females, no velvety Snout nostril distance 2 1 2 9 2 5 % 0 26 12 7% nuptial pad in male, toes webs more developed in males Between eyes 6 4 7 2 6 7 % 0 32 34 5% than in females. Three of the four females with ) ∗ + Between nostril 3 2 3 5 3 3 % 0 1 17 0% stripe and few tubercles dorsally, belly of one female with Oral disc width 4 2 4 6 4 3 % 0 15 22 2% dark masses and one white without gray nebulous mottled Snout spiracle distance 11 6 13 2 12 4 % 0 73 63 9% in preservative ( Figs 10 13 ) . Some specimens with posterior of upper lip knurled above dark brown stripe Biological data. The frog is found in the pool and with reddish yellow ( yellow ) color extending to arm pond among the wet land and forest in reproductive insertion; lower lip deeply brown stippled with whitish duration, with Sympatric distribution of Hynobius elongate mass ( Figs 2 4, 10 13) . maoershanensis at Mt. Maoer of Guangxi, South China Tadpoles ( Figs 14 17 and Table 2) . Based on six ( Zhou et al . , 2006 ) . The breeding season is from samples of tadpole series SYNU 0602001, the body pear December to February, and the eggs and tadpoles from like in dorsal profile, ovoid in lateral profile. In stage 36, stage 33 to 36 were collected in February, showing the mean length of body 19 4 mm ( 18 9 20 1 mm, n = 6) , tadpole develops overwinteringly. In March and July, mean length of tail 34 8 mm ( 31 3 39 0 mm) , body small and larger tadpoles were collected at the same width ( mean 13 62 mm) some greater than body height pond. In April, the frog moves to the land in forest and ( mean 11 55 mm) ; snout rounded, eye dorsolateral, difficult to collect. 7 98 Acta Zootaxonomica Sinica 动物分类学报 Vol. 32 No. 4
Distribution. Rana maoershanensis is currently known the Northern and Western China. from Mt. Maoer of Guangxi, China. Although this proposition is not consistent with , 3 Comparison and Discussion Dubois s who grouped the frogs based on the number of chromosomes, the size of eggs and the labial tooth row The brown frog ( also known as woodfrog) group formula of tadpole ( Dubois, 1992) . Up to now, no presents a problem most difficult to solve. Great information has been shown which one is good in confusion exists in the literature, as there has been no phylogenetics, we select Fei, s proposition ( Fei et al . , satisfactory comparative study of preserved museum 2005) here to provide a convenient, shorthand way of specimens of different species, and no careful comparing with the new species in morphological investigation in the fields ( Liu, 1946) . Eurasian brown characters. frogs are morphological conservative assemblage R . chensinensis consisting a larger number of similar species ( Kim et al . , group 2002) . In some species group, the frogs are quite similar R. maoershanensis resembles to R. chensinensis, R. in morphology, and very difficulty to identify ( Liu & dybowskii and R . kukunoris in head shape ( head width Hu, 1961; Tanaka et al . , 1996; Xie et al . , 2000; Lu equal to or larger than length) , but it differs from them & Li, 2002) . Although a great progress has been made in dorsalateral fold, vocal sac and LTRF ( slightly curved then, this phenomenon is still true for some allied brown dorsalateral fold, no internal vocal sac, and LTFR : frogs by this time ( Che, 2006; Lu & Li, 2005) . 3 3 : 1 1 and : 3 3 ! in R. maoershanensis ; Up to now, 13 species of brown frogs have been curved dorsalateral fold, internal vocal sac, LTRF : known from China. Although some of the frogs are quite 3 3 : 1 1, : 4 4 : 1 1 and : 2 2 : : 1 1 similar in morphology, they can identified from in R . chensinensis , R. dybowskii and R. kukunoris) . combination of several aspects, such as dorsalateral fold, R. maoershanensis differs from R. asiatica in internal vocal sacs, head shape, chromosome numbers dorsalateral fold, vocal sac, and middorsal line ( slightly and color characters, and labial papillae and labial tooth curved dorsalateral fold, no inner vocal sac, and no light row formula of tadpoles ( Liu & Hu, 1961; Lu & Li, middorsal line with distinct edges running from eyes to 2002; Fei et al . , 2005) . cloaca in R. maoershanensis , curved dorsaletaral fold, According to chromosome numbers, the brown reduced inner vocal sac and light middorsal line with frogs in China could be classified into two groups: one distinct edges running from eyes to cloaca present in R. group with 2n = 24 and another with 2n = 26. In 2n = 24 asiatica ) . group, the frogs mainly distribute in the North China, R . amurensis groups including R . altica , R . chensinensis , R. dybowskii , R . kukunoris and R. huanrenensis ; while in 2n = 26 group, R. maoershanensis mainly differ from R . amurensis the frogs are widespread over China, including R . species group by slightly curved dorsalateral fold ( curved zhenhaiensis, R. omeimontis , R. chaochiaoensis , R . in R. amurensis group ) , no dorsal bands or middorsal longicrus , R . asiatica , R . amurensis , R . asiatica and R . line ( Light middorsal band with distinct edges in R. kunyuensis ( no chromosome numbers data for R . asiatica , and two glandular bands in R. amurensis and R. chevronta ) . The karyotype of this new species, R . altaica) and no golden lip tripe ( golden lip tripe in R. maoershanensis is 2n = 26 ( unpublished data from super kunyuensis ) . R. maoershanensis also differs from R. altaica author, s lab. ) . by inner metatarsal tubercle much less than the length of Based on the shape of dorsolateral folds and first toe ( inner metatarsal tubercle a little less than the presence of vocal sacs, three species groups were length of first toe in R . altaica ) . proposed phenetically ( Fei et al . , 2005 ) . The R . LTRF of R . maoershanensis is different from R. longicrus group with straight or slightly curved dorsolateral amurensis group too ( more : 3 3 : 1 1 and less folds and no vocal sacs includes R. zhenhaiensis , R . : 3 3 ! in R . morershanensis; more : 1 1 and omeimontis, R . chaochiaoensis , R. longicrus and R . less : 1 1 ∋: 1 1 in R. amurensis and R . kunyuensis , chevronta , which distribute in the Southern China. This and more : 3 3 : 1 1 and less : 2 2 : : 1 1 group was formally named as R. japonica group before in R. huanrenensis ) . And the breeding season and its R . japonica in China was replaced by R . zhenhaiensis ( Xie location of R. maoershanensis much different from R. et al . , 2000; Fei et al . , 2005; Lu & Li, 2005) . The R . huanrenensis ( in winter, and egg massess not attached on chensinensis group with curved dorsolateral folds and rocks or other materials at pool and pond in R. internal vocal sacs includes R . chensinensis , R. dybowskii , maoershanensis; in Spring, and egg masses attached on R . kukunoris and R. asiatica ; and the R . amurensis group rocks in river in R . huanrenensis) . with curved dorsolateral folds and no vocal sacs includes In addition, the species in R . amurensis group other brown frogs in China. All of the frogs in R . distribute in North China, R . maoershanensis was found in chensinensis group and R . amurensis group mainly locate in South China, far away from the species in R. amurensis Oct. , 2007 陆宇燕等: 中国蛙类一新种 ( 无尾目, 蛙科) 799 group and separated by Yellow River and Yangtse River. Sichuan University. 1 81. Fei, L 1999. Atlas of Amphibians of China. Henan Scientific and R . longicrus groups Technological Publishing House, Zhengzhou. 1 432. Fei, L and Ye, C Y 2000. The Colour Handbook of the Amphibians of R . maoershanensis is similar to R. zhenhaiensis with Sichuan. China Forestry Publishing House, Beijing. 1 263. slightly curved dorsalateral folds, but differs in head Fei, L, Ye, C Y, Huang, Y Z, Jiang, J P and Xie, F 2005. An Illustrated shape ( head wider in R. maoershanensis , head longer in Key to Chinese Amphibians. Sichuan Publishing Group Sichuan , . 1 340. . ) ( Publishing House of Science and Technology Chengdu R zhenhaiensis and sexual dimorphism relative leg Gosner, K. L. 1960. A simplified table for staging anuran embryos and length of the female longer than the male and in 3 5 of larvae with notes on identification. Herpetologica , 16 (1) : 183 190. TMP: EYE in female and 1 2 in male of R . Jiang, J P and Zhou, K Y 2001. Phylogenetic relationships of Chinese brown maoershanensis; relative leg length of the female shorter frogs inferred from mitochondrial DNA sequence of 12S ribosome RNA . , 22 ( 1) : 27 32. 2 3 : . gene Zoological Research than the male and of TMP EYE in both sex of R Kim, J. B. , Min, M. S. , Yang, S. Y. and Matsui, M. 2002. Genetic zhenhaiensis) ; and LTRF of tadpoles ( more I: 3 3 : relationships among Korean brown frog species ( Anura: Ranidae) , 1 1 and less I: 3 3 ! in R. morershanensis, more I: 2 2 with special reference to evolutionary divergences between two allied ∋: 1 1 and less I: 2 2 in R . zhenhaiensis ) . species Rana dybowskki and R . huanrenensis . Zoological Science , 19 ( 3) : 369 382. . . , . R maoershanensis differs from R omeimontis R Li, A, Lu, Y Y and Li, P P 2005. Distribution of wood frogs in China. chaochiaoensis, R . longicrus and R . chevronta by slightly Sichuan Journal of Zoology , 25 ( 3) : 268 270. curved dorsalateral fold and head wider ( straight Liu, C C 1946. A new woodfrog Rana chaochiaoensis with a discussion of its dorsalateral folds and head longer in R. omeimontis , R . allied species, from West China. Journal of West China Border Research Society , 16 ( B) : 7 14. chaochiaoensis, R . longicrus and R. chevronta ) . Also R . Liu, C C and Hu, S Q 1961. Tailless Amphibians of China. Science maoershanensis differs from these brown frogs in tadpole Press, Beijing. 1 364. LTRF ( more : 3 3 : 1 1 and less : 3 3 ! in Liu, M Y, Zhang, S Q and Liu, M 1993. A new species of Ranidae from R . morershanensis , more : 2 2 : 1 1 and less : Liaoning. Acta Zootaxonomica Sinica , 18 ( 4 ) : 493 496. [ 动物 分类学 报] 3 3 : 1 1 . , : 2 2 ∋: 1 1 in R chaochiaoensis more Lu, Y Y and Li, P P 2002. A new wood frog of the genus Rana in Mt. and less : 3 3 ∋: 1 1 in R . omeimontis , and : 2 Kunyu, Shandong Province, China ( Amphibia: Anura: Ranidae) . 2 1 1: ∋ in R . longicrus and no data of R. chevronta , Acta Zootaxonomica Sinica , 27 ( 1) : 162 166. [ 动物分类学报] which has been found to be restricted to a small area in Lu, Y Y and Li, P P 2005. A brief review on advance of wood frogs research in China and discussing the importance of studying the wood Mt. Emei) . frog biodiversity around Bohai. Sichuan Journal of Zoology , 24 ( 3) : Furthermore, other different characters can be found 271 275. between R . chaochiaoensis and R . maoershanensis by Lu, Y Y, Li, P P, Jiang, D B, Zhang, J H, Luo, Y Z, and Wang, S N checking the specimens of R. chaochiaoensis ( adult, and 2006. A new record from Guangxi Rana chaochiaoensis . Sichuan Journal of Zoology , 25 ( 2) : 267. tadpole) , which were collected from its type locality and Lu, Y Y, Li, P P, Wang, W, Wang, P W and Cui, X Z 2005. The other areas at Yunnan, Guizhou and Sichuan in SYNU, species belong to Rana longicus species group in Shandong Province. CIB and KIZ. The skin is conspicuously rather smooth Sichuan Journal of Zoology , 24 ( 3) : 276 278. in R. maoershanensis ; while in R. chaochiaoensis , the skin McDiarmid, R. W. and Altig, R. 1999. Tadpoles: the Biology of Anuran Larvae. University of Chicago Press, Chicago and London. 1 444. on the back and flank is covered with tubercles or Tanaka, T. , Matsui, M. and Takenaka, O. 1996. Phylogenetic granules. In addition, the labial papillae and LTRF of relationships of Japanese brown frogs ( Rana: Ranidae) assessed by tadpoles are much stable in R . chaochiaoensis from mitochondrial cytochrome b gene sequence. Biochemical Systematics and Yunnan, Guizhou and Sichuan ( Liu & Hu, 1961; Wu et Ecology , 24 ( 2) : 299 307. Wu, L, Dong, Q and Xiu, R H 1988. The Amphibian Fauna of Guizhou. al . , 1988; Yang, 1991; Zhao & Yang, 1997; Fei & Ye, Guizhou People s Press, Guiyang. 1 192. 2000) , which is different from this new species in absence Xie, F, Fei, L and Ye, C Y 2000. On taxonomic status and relationship of of more than one papillae in midst of lower lip in R . Rana japonica group, in China ( Amphibia: Anura: Radidae) . Cultum chaochiaoensis tadpoles ( papillae of lower lip regularly Herpetologica Sinica , 8: 74 80. Yang, D T 1991. Amphibian Fauna of Yunnan. China Forestry Press, arranged in R. maoershanensis tadpoles) . Beijing. 1 259. Acknowledgements We are very grateful to WU Yang, X G, Wang, Y Q, Zhou, K Y and Liu, Z Q 2001. Phylogenetic relationships of Chinese brown frogs ( Rana ) based on sequence of Guan Fu, ZHAO Hui, WANG Yue Zhao and LI mitochondrial cytochrome b gene. Zoological Research , 22 ( 4) : 345 Sheng Quan of Chengdu Inistitute of Biology, Chinese 350. Academy of Sciences, RAO Ding Qi of Kunming Ye, C Y, Fei, L and Hu, S Q 1993. Rare and Economic Amphibians of , , China. Sichuan Science and Technology Publishing House, Chengdu. Institute of Zoology Chinese Academy of Sciences and 1 412. WANG Shao Neng and other members from Ye, C Y, Fei, L and Matsui, M. 1995. Taxonomic study on Chinese Rana Management Bureau of Maoershan National Nature japonica . Acta Herpetologica Sinica , 4 5: 82 87. Reserve for their help in field surveys and specimens Zhao, E M and Yang, D T 1997. Amphibians and Reptiles an the . , . 1 303. . Hengduanshan Mountains Region Science Press Beijing observation Zhou, F, Jiang, A W and Jiang, D B 2006. A new species of the genus REFERENCES Hynobius from Guangxi Zhuang Autonomous Region, China ( Caudata, Hynobiidae) . Acta Zootaxonomica Sinica, 31 ( 3) : 670 674. [ 动物分类 Che, J 2006. Molecular Phylogenetics of Living Ranids. Ph D thesis. 学报] 8 00 Acta Zootaxonomica Sinica 动物分类学报 Vol. 32 No. 4
中国蛙类一新种 ( 无尾目, 蛙科)
陆宇燕1 李丕鹏1 蒋得斌2 1. 沈阳师范大学两栖爬行动物多样性研究组 沈阳 110034 2. 猫儿山国家级自然保护区管理局 桂林 541316
摘 要 描述了 1 种采自广西 猫儿山国家 级自然 保护区 的蛙 新种在头型上与中国林蛙相 似, 在体形 上与昭觉 林蛙相 类 1 新种, 模式标本保存于沈阳师范大学自然博 物馆。 似。但与已知林蛙 均有 明显 不同。新 种的 背侧 褶略 显弯 曲, 这一特点明显不同于主要分布于 中国北方 的黑龙江林 蛙种组 猫儿山林蛙, 新种 Rana ma oershanensis sp. nov. ( 图 1~ 17) 和中 国林 蛙种组 . 的成 正模: SYNU 06020120, 雄 性成 体, 李 丕 鹏 和 王绍 能 于 Rana amurensis group R chensinensis group 员。新种与 长肢 林 蛙种 组 R . longicrus group 的镇 海 林 蛙 R . 2006 年 2 月 采集 自 广西 壮族 自 治区 猫儿 山 国家 级 自 然保 护 在背侧褶的弯曲特点上相似 , 但前者 有别于后者的 区, 海拔 1 980 m。配 模: SYNU 06020091, SYNU 06020122 和 zhenhaiensis 主要特点有: 头型较宽扁, 雌蛙后 腿相对 较长, 蝌蚪 唇齿式 SYNU 06020124, 雄性成 体; SYNU 06020121, SYNU 06040153, 多为 : 3 3 : 1 1。新种与 长肢林 蛙种 组的其 他林 蛙不仅 SYN 0604154 和 SYNU 06040155, 雌性成体。李丕鹏和 王绍能 在背侧褶和头型等方面明显有 别 而且蝌蚪 唇齿式也 显著不 于 2006 年 2 月 和 4 月 采集, 采 集 地 点 与正 模 相 同。 蝌 蚪: , 同。通过对四川、贵 州和 云南的 昭觉 林蛙 . 成 SYNU 0602001 和 SYNU 0604001 系 列, 采集 信息 与配 模 标本 R chaochiaoensis 体和蝌蚪标本 包括地模标本 的核查和 比较 新种 的胫跗 相同。 ( ) , 关节前达眼角 皮肤 光滑 缺乏 疣粒 雄性 腹 侧无 雄性 线 新种具有如下主要鉴 别特 征: 1) 体 略大; 2) 头宽 略大 , , , , 蝌蚪下唇乳突排列规则 中央无 缺刻 而昭 觉林蛙胫 跗关节 于头长; 3) 背 侧褶 明 显且 略弯 曲; 4) 胫 跗关 节前 达 眼 角; , ; 前达鼻孔或超过吻端, 体侧和背 部有圆疣 或长疣, 雄 性背腹 5) 雄性婚垫 2 团, 无内声囊, 腹部无雄性线; 6) 蝌蚪唇齿式 侧均有雄性线 蝌蚪下唇中央乳头排列稀疏或有缺刻。 多为 : 3 3 : 1 1, 少数为 : 3 3 !。 ,
关键词 无尾类, 蛙科, 猫儿山林蛙, 新种, 中国. 中图分类号 Q959 53 Appendix specimens examined CIB37755, CIB37756, CIB37758, CIB37760; ( females ) CIB37720, Rana altaica from SYNU ( 7) . China, Xinjiang Uygur Autonomous CIB37721, CIB37724, CIB37725, CIB37773, CIB37777. China, Yunnan Region, Jingbuer River: ( males ) SYNU050204, SYNU050205, Province, Kunming City: ( males) CIB37796, CIB37799, CIB37798, SYNU050206; ( females ) SYNU050200, SYNU050201, SYNU050202, CIB37801, CIB37802, CIB37803, CIB37804, CIB37805, CIB37806, SYNU050203. ( females ) CIB37786, CIB37787, CIB37788, CIB37790, CIB37792, Rana amurensis from SYNU ( 16 ) . China, Jilin Province: ( males) CIB37793, CIB37830. China, Yunnan Province, Lijiang County: SYNU06050247, SYNU06050249, SYNU06050252, SYNU06050254, ( males) : CIB37840, CIB37839; ( female) CIB37838. SYNU06050257, SYNU06050258, SYNU06050260, SYNU06050261; Rana chensinensis from SYNU ( 12 ) . China, Shaanxi Province, Hu ( females ) SYNU050144, SYNU06050248, SYNU06050250, County (type locality) : ( males) SYNU050261, SYNU050262; ( females) SYNU06050251, SYNU06050253, SYNU06050255, SYNU06050256, SYNU050263, SYNU050264. China, Beijing: ( female) SYNU06090564. SYNU06050259. China, Henan Province: ( males ) SYNU06100578, SYNU06100587, Rana asiatica from SYNU ( 7) . China, Xinjiang Uygur Autonomous SYNU06100589, SYNU06100599: ( females ) SYNU06100574, Region, Yili County: ( subadults ) SYNU050211, SYNU050212, SYNU06100576, SYNU06100600. SYNU050213, SYNU050214, SYNU050215, SYNU050216, SYNU050217. Rana chevronta from CIB ( 1) . China, Sichuan Province, Emei Mt. Rana chaochoensis from KIZ ( 29) . China, Yunnan Province, Chuxiong ( type locality) : ( male) CIB65I0028. and Zhongdian Counties: ( females ) 8409024, 82115. China, Yunnan Rana dybowskii from SYNU ( 34 ) . China, Jilin Province, Changbai Province, Shuangbai and Yangbi Counties: ( males) 840921, 820915, Mt. : ( males ) SYNU050100, SYNU050102, SYNU06050281, 820919, 820923, 820929; ( females ) 820953, 820954. China, Yunnan SYNU06050282; ( females ) SYNU050103, SYNU06050280. China, Province, Weixing and Ludian County: ( males) 82I201, 82I202, 82I363; Heilongjiang Province, Shangzhi County: ( male) SYNU06060407; ( females ) 82I200, 82I303. China, Yunnan Province, Ludian County: (female) SYNU06060408. China, Liaoning Province, Xiuyuan, Beining ( males) 1033, 1035, 1034, 1036, 1039, 1093; ( females ) 0866, 1067, and Lingyuan Counties: ( males ) SYNU050229, SYNU050243, 1103. China, Yunnan Province, Lijiang ( KIZ, Dr. Rao, s Lab) : ( males) SYNU050244, SYNU050247, SYNU050248, SYNU06040176, IOZCAS 4282, IOZCAS 4283, IOZCAS 4284, IOZCAS 4285, IOZCAS SYNU06040178, SYNU06040180, SYNU06040181, SYNU06040182; 4288, IOZCAS 4291. ( females ) SYNU050227, SYNU050232, SYNU050233, SYNU050234, Rana chaochoensis from CIB ( 70) . China, Sichuan Province, Zhaojue SYNU050235, SYNU050236, SYNU050237, SYNU050241, SYNU050242, County ( as Chaocho formerly, type locality) : ( males ) CIB37841, SYNU050245, SYNU050246, SYNU050249, SYNU050250, SYNU050251, CIB37842, CIB37847, CIB37850, CIB37851, CIB37852, CIB37855, SYNU050252, SYNU050253. CIB37857, CIB37859, CIB37866, CIB37867, CIB37870, CIB37873, Rana huanrenensis from SYNU ( 7) . China, Liaoning Province, Huanren CIB37877; ( females ) CIB378853, CIB37860, CIB37862, CIB37869, County ( type locality) : ( males ) SYNU050231, SYNU050232, CIB37874, CIB37876, CIB84495. China, Sichuan Province, Yuexi, SYNU050233, SYNU050234; ( females ) SYNU050237, SYNU050238, Mianning, and Yanyuan Counties: ( males ) CIB37643, CIB37683, SYNU050239. CIB37703; ( females ) CIB37641, CIB37642, CIB37644, CIB 37681, Rana kukunoris from SYNU ( 4) . China, Gansu Province: ( males) CIB37699, CIB37700, CIB37707. China, Guizhou Province, Weining SYNU06080510, SYNU06080512, SYNU06080513; ( female ) County: ( males ) CIB37712, CIB37713, CIB37715, CIB37723, SYNU06080511. CIB37731, CIB37734, CIB37735, CIB377747, CIB37748, CIB37754, Rana kunyusnesis from SYNU ( 11) . China, Shandong Province, Kunyu Oct. , 2007 陆宇燕等: 中国蛙类一新种 ( 无尾目, 蛙科) 801
Mt ( type locality ) : ( males ) SYNU06040204, SYNU06040205, Rana zhenhaiensis from SYNU ( 18) . China, Zhejiang Province, Beilun SYNU06040206, SYNU06040207, SYNU06040208, SYNU06040209, Region ( formerly Zhenhai County, type locality) : ( males) SYNU050267, SYNU06040210, ( females ) SYNU06040211, SYNU06040212, SYNU050268, SYNU050271, SYNU06020126, SYNU06040129, SYNU06040213, SYNU06040214. SYNU06040130, SYNU06040131, SYNU06040132, SYNU06040133, Rana omeimontis from SYNU ( 8) . China, Sichuan Province, Emei Mt. SYNU06040134, SYNU06040135; (females) SYNU050269, SYNU050270, ( type locality) : ( males ) SYNU050274, SYNU050275, SYNU050278, SYNU050272, SYNU050273, SYNU06020127, SYNU06020128, SYNU06080522, SYNU06080523; ( females) SYNU050276, SYNU050277, SYNU06040136. SYNU050279.