AFG3L2 Antibody (R32419)

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AFG3L2 Antibody (R32419) Catalog No. Formulation Size R32419 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug Bulk quote request Availability 1-3 business days Species Reactivity Human, Mouse, Rat Format Antigen affinity purified Clonality Polyclonal (rabbit origin) Isotype Rabbit IgG Purity Antigen affinity Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide UniProt Q9Y4W6 Applications Western blot : 0.1-0.5ug/ml Immunofluorescence (FFPE) : 2-4ug/ml Flow cytometry : 1-3ug/million cells Limitations This AFG3L2 antibody is available for research use only. Immunofluorescent staining of FFPE human U-2 OS cells with AFG3L2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min. Flow cytometry testing of human A431 cells with AFG3L2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AFG3L2 antibody. Western blot testing of 1) rat brain, 2) mouse spleen and 3) human U87 lysate with AFG3L2 antibody. Expected molecular weight ~89 kDa. Description AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly. In humans, it is encoded by the AFG3L2 gene. This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. And this gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders as well as spastic ataxia-neuropathy syndrome. Application Notes Optimal dilution of the AFG3L2 antibody should be determined by the researcher. Immunogen Amino acids R168-D250 of the human protein were used as the immunogen for the AFG3L2 antibody. Storage After reconstitution, the AFG3L2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing. Ordering: Phone:858.663.9055 | Fax:1.267.821.0800 | Email:[email protected] Copyright © NSJ Bioreagents. All rights reserved.

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18P Deletions FTNW
18p deletions rarechromo.org 18p deletions A deletion of 18p means that the cells of the body have a small but variable amount of genetic material missing from one of their 46 chromosomes – chromosome 18. For healthy development, chromosomes should contain just the right amount of material – not too much and not too little. Like most other chromosome disorders, 18p deletions increase the risk of birth defects, developmental delay and learning difficulties. However, the problems vary and depend very much on what genetic material is missing. Chromosomes are made up mostly of DNA and are the structures in the nucleus of the body’s cells that carry genetic information (known as genes), telling the body how to develop, grow and function. Base pairs are the chemicals in DNA that form the ends of the ‘rungs’ of its ladder-like structure. Chromosomes usually come in pairs, one chromosome from each parent. Of these 46 chromosomes, two are a pair of sex chromosomes, XX (a pair of X chromosomes) in females and XY (one X chromosome and one Y chromosome) in males. The remaining 44 chromosomes are grouped in 22 pairs, numbered 1 to 22 approximately from the largest to the smallest. Each chromosome has a short ( p) arm (shown at the top in the diagram on the facing page) and a long ( q) arm (the bottom part of the chromosome). People with an 18p deletion have one intact chromosome 18, but the other is missing a smaller or larger piece from the short arm and this can affect their learning and physical development.
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