Polyclonal Antibody to MUL1 / RNF218 (C-Term) - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP52776PU-N Polyclonal Antibody to MUL1 / RNF218 (C-term) - Aff - Purified

Alternate names: C1orf166, E3 ubiquitin-protein ligase MUL1, GIDE, Growth inhibition and death E3 ligase, MAPL, MULAN, Mitochondrial ubiquitin ligase activator of NFKB 1, Mitochondrial-anchored protein ligase, Putative NF-kappa-B-activating protein 266, RING finger protein 218 Quantity: 0.4 ml Concentration: 0.5 mg/ml (lot specific) Background: E3 ubiquitin-protein ligase that plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Uniprot ID: Q969V5 NCBI: NP_078820 GeneID: 79594 Host / Isotype: Rabbit / Ig Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 272-301 aa) of human MUL1 / RNF218 Format: State: Liquid purified Ig fraction Purification: Purified through a Protein A column followed by peptide affinity purification Buffer System: PBS with 0.09% (W/V) Sodium azide Applications: Western blot: 1/1000. Enzyme immunoassay: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody recognizes MUL1 / RNF218 at C-term. Species Reactivity: Tested: Human Add. Information: Molecular Weight: 39800 Da Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: Laure, L. et al., FEBS J. 277(20): 4322-4337 (2010) Braschi, E. et al., EMBO Rep. 10(7): 748-754 (2009) Venkatesan, K. et al., Nat. Methods 6(1): 83-90 (2009) Zhang, B. et al., Cell Res. 18(9): 900-910 (2008) Zhang, H. et al., Biochem. Biophys. Res. Commun. 366(4): 898-904 (2008)

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MT/20150528 1 / 2 AP52776PU-N: Polyclonal Antibody to MUL1 / RNF218 (C-term) - Aff - Purified

Pictures: Western blot analysis in human placenta tissue lysates (35ug/lane) using MUL1 / RNF218 antibody Cat.-No. AP52776PU-N (C-term). This demonstrates this antibody detected the MUL1 protein (arrow).

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MT/20150528 2 / 2