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1 Studies on Two Genomic Variants of Taura Studies on Two Genomic Variants of Taura Syndrome Virus: Infection under Hyperthermic Conditions and Detection with a Novel Monoclonal Antibody Item Type text; Electronic Dissertation Authors Cote, Isabelle Publisher The University of Arizona. Rights Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. Download date 09/10/2021 11:46:35 Link to Item http://hdl.handle.net/10150/195556 1 STUDIES ON TWO GENOMIC VARIANTS OF TAURA SYNDROME VIRUS: INFECTION UNDER HYPERTHERMIC CONDITIONS AND DETECTION WITH A NOVEL MONOCLONAL ANTIBODY by Isabelle Côté __________________________________ A Dissertation Submitted to the Faculty of the DEPARTMENT OF VETERINARY SCIENCE AND MICROBIOLOGY In Partial Fulfilment of the Requirements For the Degree of DOCTOR OF PHILOSPHY WITH A MAJOR IN MICROBIOLOGY In the Graduate College THE UNIVERSITY OF ARIZONA 2008 2 THE UNIVERSITY OF ARIZONA GRADUATE COLLEGE As members of the Dissertation Committee, we certify that we have read the dissertation prepared by Isabelle Côté entitled: "Studies on Two Genomic Variants of Taura Syndrome Virus: Infection under Hyperthermic Conditions and Detection with a Novel Monoclonal Antibody” and recommend that it be accepted as fulfilling the dissertation requirement for the Degree of Doctor of Philosophy. _______________________________________________ Date: __06/09/2008_______ Donald V. Lightner, Ph.D. _______________________________________________ Date: __06/09/2008_______ Bonnie T. Poulos, Ph.C. _______________________________________________ Date: __06/09/2008_______ Michael A. Cusanovich, Ph.D. _______________________________________________ Date: __06/09/2008_______ Carol L. Dieckmann, Ph.D. _______________________________________________ Date: __06/09/2008_______ Michael W. Riggs, DVM, Ph.D. Final approval and acceptance of this dissertation is contingent upon de candidate's submission of the final copies of the dissertation to the Graduate College. I hereby certify that I have read this dissertation prepared under my direction and recommend that it be accepted as fulfilling the dissertation requirements. _______________________________________________ Date: __06/09/2008_______ Dissertation Director: Donald V. Lightner 3 STATEMENT BY AUTHOR This dissertation has been submitted in partial fulfilment of requirements of an advanced degree at The University of Arizona and is deposited in the University Library to be made available to borrowers under rules of the library. Brief quotations from this dissertation are allowable without special permission provided that accurate acknowledgement of source is made. Requests for permission for extended quotation from or reproduction of this manuscript in whole or in part may be granted by the Head of the Department of Veterinary Science and Microbiology or the Dean of the Graduate College when in his or her judgement the proposed use of the material is in the interest of scholarship. In all other instances, however, permission must be obtained from the author. SIGNED:______________________ Isabelle Côté 4 ACKNOWLEDGEMENTS Many thanks to Dr. Donald Lightner, who welcomed me in his laboratory and supported all the work presented here. Dr. Lightner's professionalism and outstanding qualities as a researcher and a pathologist have set an amazing example allowing me to grow as an aquatic animal health professional and a science person. Heartfelt acknowledgements are due to Bonnie Poulos, whose tireless work and constant joie de vivre have been tremendous blessings throughout the course of this project. To my graduate committee members: Dr Michel Riggs, Dr. Michael Cusanovich and Dr. Carol Dieckmann. Your guidance and advices have been essential in shaping and completing this work. I am very fortunate to have had the opportunity to work with a group of dedicated and amazing people. Present and past lab members have taught me many things about scientific thought, shrimp diseases, and even life in general. Specific thanks are extended to Allan Heres, Brenda White, Dr. Carlos Pantoja, Debbie Bradley-Dunlop, Dr. Kathy Tang, Linda Nunan, Leone Mohney, Paul Schofield, Rita Redman, Thales Andrade, Thiannarat Srisuvan and Wanda McCormack. All of whom, to one extent or another, contributed to this work. Michael Anderson and Dr. Helen Jost provided much appreciated help with experimental animals: Holly, Molly, Polly and Dolly (I and II). Dr. Janet Decker opened to me the doors of the wonderful world of teaching. Financial support for this research was provided by the United States Marine Shrimp Farming Consortium under the grant: 2004-38808-02142 from the Cooperative State Research, Education and Extension Service, U.S. Department of Agriculture. The Department of Veterinary Sciences and Microbiology as well as the Department of Ecology and Evolutionary Biology have provided teaching and graduate research assistantships. To Philip, for making it all worthwhile. 5 DEDICATION To the shrimp and mice who gave their life for the advancement of science. You will be remembered. 6 TABLE OF CONTENTS Page LIST OF FIGURES.............................................................................................................8 LIST OF TABLES...............................................................................................................9 ABSTRACT.......................................................................................................................10 CHAPTER 1. Literature review.........................................................................................12 Where it all began... the controversy...............................................................…..12 Identification and description of the virus.............................................................14 Variants of the virus...............................................................................................17 Geographic distribution.........................................................................................19 Species of shrimp susceptible................................................................................20 Pathology and disease cycle...................................................................................23 Cellular biology of the disease...............................................................................26 Routes of transmission...........................................................................................27 Diagnostic methods................................................................................................29 Methods of control.................................................................................................33 CHAPTER 2. Development and characterization of a Monoclonal antibody against Taura syndrome virus of penaeid shrimp.....................................................................................39 Abstract..................................................................................................................39 Introduction............................................................................................................40 Material and Methods............................................................................................42 Results....................................................................................................................48 Discussion..............................................................................................................50 CHAPTER 3. Taura Syndrome Virus from Venezuela is a New Genetic Variant............59 Abstract..................................................................................................................59 Introduction............................................................................................................60 Material and Methods............................................................................................62 Results....................................................................................................................67 Discussion..............................................................................................…............69 CHAPTER 4. Hyperthermia protects Kona stock Penaeus vannamei against infection by TSV-HI but not TSV-BZ...................................................................................................80 Abstract..................................................................................................................80 Introduction............................................................................................................81 Material and Methods............................................................................................83 Results................................................................................................................... 86 Discussion........................................................................................................…..88 7 TABLE OF CONTENTS - Continued REFERENCES........................................................................................................……98 8 LIST of FIGURES Figure Page 1.1 Transmission electronic micrograph of TSV………………………...………………35 1.2 Schematic diagram of the genomic organization of TSV…………………….……...36 1.3
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