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Principles of Fluorescence Spectroscopy Principles of Fluorescence Spectroscopy Third Edition Principles of Fluorescence Spectroscopy Third Edition Joseph R. Lakowicz University of Maryland School of Medicine Baltimore, Maryland, USA Joseph R. Lakowicz Center for Fluorescence Spectroscopy University of Maryland School of Medicine Baltimore, MD 21201 USA Library of Congress Control Number: 2006920796 ISBN-10: 0-387-31278-1 ISBN-13: 978-0387-31278-1 Printed on acid-free paper. © 2006, 1999, 1983 Springer Science+Business Media, LLC All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed in Singapore. (KYO) 987654321 springer.com Dedicated to Mary, for her continuous support and encouragement, without whom this book would not have been written Preface The first edition of Principles was published in 1983, and adigm of fluorescence, from a reliance on organic fluo- the second edition 16 years later in 1999. At that time I rophores, to the use of genetic engineering, nanotechnolo- thought the third edition would not be written until 2010 or gy, and near-field optics. later. However, the technology of fluorescence has I wish to express my appreciation and special thanks to advanced at an accelerating pace. Single-molecule detec- the individuals who have assisted me in the preparation of tion and fluorescence-correlation spectroscopy are becom- the book. These include Ignacy Gryczynski for assistance ing almost routine. New classes of probes have appeared, with the figures, Krystyna Gryczynski for drawing the fig- such as the semiconductor nanoparticles, or QDots, and ures, Joanna Malicka for proofreading the chapters, Kazik genetically engineered green fluorescent probes. Addition- Nowaczyk for the cover design and color digitizing of all ally, it is now becoming possible to control the excited figures, Tim Oliver for typesetting, and the NIH for their states of fluorophores, rather than relying only on sponta- support of my laboratory. And finally, Mary, for her endless neous emission. These developments are changing the par- hours of typing, correspondence and support. Joseph R. Lakowicz vii Glossary of Acronyms A acceptor C102 coumarin 102 AA anthranilic acid C152 coumarin 152 2-AA 2-acetylanthracene C153 coumarin 153 Ac acetonitrile 9-CA 9-cyanoanthracene Ac acetone or acridine CaM calmodulin ACF acriflavine cAMP cyclic AMP AcH acridinium cation CFD constant fraction discriminator ACTH adrenocorticotropin hormone CG calcium green Alexa-Bz Alexa-labeled benzodiazepine CHO Chinese hamster ovary ADC analog-to-digital converter CC closed circular Adx adrenodoxin CCDs charged-coupled devices I-AEDANS 5-((((2-iodoacetyl)amino)ethyl)amino)- CH cyclohexane naphthalene-1-sulfonic acid Chol cholesterol AFA aminofluoranthene CLSM confocal laser scanning microscopy AN anthracene CNF carboxynaphthofluorescein 2-AN 2-anilinonaphthalene ConA concanavalin A 2,6-ANS 6-(anilino)naphthalene-2-sulfonic acid CRABPI cellular retinoic acid binding protein I AO acridine orange or acoustooptic CSR continuous spectral relaxation 2-AP 2-aminopurine CT charge transfer 4-AP 4-aminophthalimide CW continuous wave APC allophycocyanin APDs avalanche photodiodes D donor 9-AS 9-anthroyloxy stearic acid Dansyl 5-dimethylaminonaphthalene-1-sulfonic acid ASEs asymptotic standard errors DAPI 4',6-diamidino-2-phenylindole AT antithrombin DAS decay-associated spectra DBS 4-dimethylamino-4'-bromostilbene B benzene DC deoxycytosine BABAPH 2-(sulfonatobutyl)-7-(dibutylamino)-2-aza- DDQ distance-dependent quenching phenanthrene DEA diethylaniline BABP sulfonatobutyl)-4-[4'-(dibutylamino)- DEE diethyl ether phenyl]pyridine DHE dihydroequilenin BCECF 7'-bis(2-carboxyethyl)-5(6)-carboxyfluores- DHP dihexadecyl phosphate cein DiI or DiIC12 1,1'-didodecyl-3,3,3',3'-tetramethy lindo- BSA bovine serum albumin carbocyanine BODIPY refers to a family of dyes based on 1,3,5,7,8- DM dodecylmaltoside pentamethyl pyrromethene-BF2, or 4,4- DMA dimethylaniline difluoro-4-bora-3a,4a-diaza-s-indacene; DMAS N-dimethylaniline sulfonate BODIPY is a trademark of Molecular DMF dimethylformamide Probes Inc. DMPC dimyristoyl-L-α-phosphatidylcholine β-PE β-phycoerythrin DMP dimethyldiazaperopyrenium BPTI bovine pancreatic trypsin inhibitor DMSO dimethyl sulfoxide Bromo-PCs brominated phosphatidylcholines DMQ 2,2'-dimethyl-p-quaterphenyl Bu butanol 10-DN 10-doxylnonadecane ix x GLOSSARY OF ACRONYMS DNS dansyl or 4-dimethylamino-4'-nitrostilbene GPD glyceraldehyde-3-phosphate dehydrogenase DNS-Cl dansyl chloride GPI glycosylphosphatidylinositol DOS trans-4-dimethylamino-4'-(1-oxobutyl) GuHCI guanidine hydrochloride stilbene GUVs giant unilamellar vesicles DPA 9,10-diphenylanthracene DPA dipicolinic acid H n-hexane DPE dansyl-labeled phosphatidylethanolamine HDL high-density lipoprotein DPH 1,6-diphenyl-1,3,5-hexatriene HeCd helium–cadmium DPO 2,5-diphenyloxazole HG harmonic generator DPPC dipalmitoyl-L-α-phosphatidylcholine HITCI hexamethylindotricarbocyanine iodide DPPC dipalmitoylphosphatidylcholine HLH human luteinizing hormone DP(M,O)PC(E) dipalmitoyl(myrisotyl, oleayl)-L-α- HO highest occupied phosphatidylcholine (ethanolamine) HpRz hairpin ribozyme DTAC dodecyltrimethylammonium chloride HPTS 1-hydroxypyrene-3,6,8-trisulfonate hrIFN-γ human recombinant interferon γ EA ethyl acetate HSA human serum albumin EA ethanol 17β-HSD 17β-hydroxysteroid dehydrogenase EAN ethylaniline hw half-width EB ethidium bromide EC ethylcellulose IAEDANS 5-(((2-iodoacetyl)amino)ethyl)amino)- ECFP enhanced cyan fluorescent protein naphthalene-1-sulfonic acid EDT 1,2-ethanedithiol IAF 5-iodoacetamidofluorescein EG ethylene glycol ICT internal charge transfer ELISA enzyme-linked immunoadsorbent assays IM insertion mutant eosin-PE eosin-phosphatidylethanolamine Indo-1-C18 indo-1 with a C18 chain EP 1-ethylpyrene IRF instrument response function EPE eosin-labeled phosphatidylethanolamine IXP isoxanthopterin ESIPT excited-state intramolecular proton transfer ESR excited-state reaction KF Klenow fragment EO electrooptic KSI 3-ketosteroid isomerase EYFP enhanced yellow fluorescent protein LADH liver alcohol dehydrogenase F single-letter code for phenylalanine LCAT lecithin:cholesterol acyltransferase Fl fluorescein LDs laser diodes Fl-C fluorescein-labeled catalytic subunit LE locally excited FABPs fatty acid binding proteins LEDs light-emitting diodes FAD flavin adenine dinucleotide LU lowest unoccupied FC fura-2 with calcium FCS fluorescence correlation spectroscopy M monomer FD frequency domain MAI N-methylquinolinium iodide Fn fibronectin MBP maltose-binding protein Fs femtosecond MCA multichannel analyzer FITC fluorescein-5-isothiocyanate MCP microchannel plate FLIM fluorescence-lifetime imaging microscopy Me methanol FMN flavin mononucleotide MEM method-of-moments FR folate receptor met RS methionyl-tRNA synthetase FRET fluorescence-resonance energy transfer 3-MI 3-methyl indole FWHM full width of half-maximum intensity MLC metal–ligand complex, usually of a transition 4FW 4-fluorotryptophan metal, Ru, Rh or Os MLCK myosin light chain kinase GADPH glyceraldehyde-3-phosphate dehydrogenase MLCT metal–ligand charge transfer (state) GFP green fluorescent protein MLE maximum likelihood estimates GGBP glucose-galactose binding protein MPE multiphoton excitation GM Goppert-Mayer MPM multiphoton microscopy GOI gated optical image intensifier MQAE 6-methoxy-quinolyl acetoethyl ester GP generalized polarization MRI magnetic resonance imaging PRINCIPLES OF FLUORESCENCE SPECTROSCOPY xi NADH reduced nicotinamide adenine dinucleotide QDs quantum dots NATA N-acetyl-L-tryptophanamide QTH quartz–tungsten halogen NATyrA N-acetyl-L-tyrosinamide NB Nile blue RBC radiation boundary condition NBD N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) RBL rat basophilic leukemia NBD-DG 1-oleoyl-2-hexanoyl-NBD-glycerol R-PE R-phycoerythrin Nd:YAG neodymium:YAG REES red-edge excitation shifts NIR near infrared Re I rhenium NLLS nonlinear least squares RET resonance energy transfer NMA N-methylanthraniloyl amide RF radio frequency NO nitric oxide RFP red fluorescent protein NPN N-phenyl-1-naphthylamine Rh rhodamine NR neutral red RhB rhodamine B NRP neuronal receptor peptide RhG rhodamine green 5-NS 5-doxylstearate R6G rhodamine 6G RNase T1 ribonuclease T1 OG Oregon green RR rhodamine red OPO optical parameter oscillator Ru ruthenium ORB octadecyl rhodamine B Os osmium SAS species-associated spectra SBFI sodium-binding benzofuran isophthalate PBFI potassium-binding benzofuran isophthalate SBP steroid-binding protein PC phosphatidylcholine SBS substrate-binding strand PCSC photon-counting streak camera SC subtilisin Carlsberg PDA pyrene dodecanoic acid SDS sodium dodecylsulfate PDs photodiodes SEDA dapoxyl sulfonyl ethylenediamine PE phycoerythrin SMD single-molecule detection PE phosphatidylethanolamine SNAFLs seminophthofluoresceins 1PE one-photon SNARFs seminaphthorhodafluors 2PE two-photon SP short-pass 3PE three-photon SPQ
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