Human 2/PRSS2 Antibody

Monoclonal Mouse IgG1 Clone # 410322 Catalog Number: MAB3586

DESCRIPTION Species Reactivity Human Specificity Detects human Trypsin 2/PRSS2 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) or rhTrypsin 3. Recognizes both the pro and mature forms of rhTrypsin 2.

Source Monoclonal Mouse IgG1 Clone # 410322 Purification A or G purified from hybridoma culture supernatant Immunogen Mouse myeloma cell line NS0-derived recombinant human Trypsin 2/PRSS2 Ala16-Ser247 Accession # P07478 Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

APPLICATIONS Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website. Recommended Sample Concentration Western Blot 2 µg/mL Human Tissue and Recombinant Human Trypsin 2/PRSS2 under non-reducing conditions only Immunoprecipitation 12.5 µg/500 µg cell lysate Human pancreas tissue

DATA

Western Blot Western Blot Detection of Human Trypsin 2/PRSS2 by Detection of Human Trypsin 2/PRSS2 by Western Blot. Western blot shows lysates of Western Blot. Western blot shows human pancreas tissue. PVDF membrane recombinant human Trypsin 1, recombinant was probed with 2 µg/mL of Mouse Anti- human Trypsin 2 and recombinant human Human Trypsin 2/PRSS2 Monoclonal Trypsin 3. PVDF membrane was probed with Antibody (Catalog # MAB3586) followed by 2 µg/mL of Mouse Anti-Human HRP-conjugated Anti-Mouse IgG Secondary Trypsin 2/PRSS2 Monoclonal Antibody Antibody (Catalog # HAF018). A specific (Catalog # MAB3586) followed by HRP- band was detected for Trypsin 2/PRSS2 at conjugated Anti-Mouse IgG Secondary approximately 24 kDa (as indicated). This Antibody (Catalog # HAF018). A specific experiment was conducted under non- band was detected for Trypsin 2/PRSS2 at reducing conditions and using Western Blot approximately 24 kDa (as indicated). This Buffer Group 1. experiment was conducted under non- reducing conditions and using Western Blot Buffer Group 1.

Immunoprecipitation Detection of Human Trypsin 2/PRSS2 by Immunoprecipitation. Human Trypsin 2/PRSS2 was immunoprecipitated from 500 μg of human pancreas lysates with 12.5 ug Mouse Anti-Human Trypsin 2/PRSS2 Monoclonal Antibody (Catalog # MAB3586). The Trypsin 2/PRSS2-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated human Trypsin 2/PRSS2 was detected by Western blot using 2 µg/mL of Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3586) under non-reducing conditions and using Western Blot Buffer Group 1.

PREPARATION AND STORAGE Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS. Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (-SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Rev. 9/24/2020 Page 1 of 2 Human Trypsin 2/PRSS2 Antibody

Monoclonal Mouse IgG1 Clone # 410322 Catalog Number: MAB3586

BACKGROUND Human Trypsin 2, encoded by the PRSS2 , is also known as anionic Trypsin (1). Accounting for approximately 1/3 of the total trypsin content in the pancreatic juice, it is one of the trypsins produced in the pancreas. It consists of a signal peptide (residues 1-15), a pro region (residues 16-23), and a mature chain (residues 24- 247). Trypsin 2 is synthesized as a zymogen and secreted into the duodenal lumen, where it is activated by enteropeptidase (2). It plays a central role in digestion and activating other pro-enzymes. In human pancreatic diseases and chronic alcoholism, it is up-regulated (3).

References: 1. Emi, M. et al. (1986) Gene. 41:305. 2. Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 1483-1488, Academic Press, San Diego. 3. Rinderknecht, H. et al. (1979) Gut. 20:886.

Rev. 9/24/2020 Page 2 of 2