Information Summary of Selected Core Genes and Alternative Genes. in Activity Column, MME Represent Mo

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Supplementary material 1: Information summary of selected core genes and alternative genes. In Activity column, MME represent monosaccharide metabolic enzymes, a: enzyme family from Pfam database, b: enzyme family from CAZY database. c: cut value that used for corresponding model. polysaccharide activity Classification Enzyme family Rebuild model? cut_valuec MME Core gene PF00171a Y 1.00E-100 MME Core gene PF13378 a Y 1.00E-50 MME Core gene PF08240 a Y 1.00E-50 MME Core gene PF 00106 a Y 1.00E-50 agarase Alternative gene GH16b Y 1.00E-25 Agar agarase Alternative gene GH86 b Y 1.00E-20 agarase Alternative gene GH117 b Y 1.00E-20 galactosidase Alternative gene GH2 b Y 1.00E-50 agarase Alternative gene GH96 b Y 1.00E-10 agarase Alternative gene GH118 b Y 1.00E-10 MME Core gene PF00171 a Y 1.00E-50 MME Core gene PF13378 a Y 1.00E-50 carrageenan carrageenase Alternative gene GH82 b Y 1.00E-20

carrageenase Alternative gene GH16 b Y 1.00E-50 carrageenase Alternative gene GH150 b Y 1.00E-50 MME Core gene PF02614 a N 1.00E-10 MME Core gene PF04295 a N 1.00E-10 MME Core gene PF08125 a N 1.00E-10 pectatelyase Alternative gene PL1 b Y 1.00E-10 pectin pectatelyase Alternative gene PL10 b Y 1.00E-10

pectatelyase Alternative gene PL9 b Y 1.00E-50 pectatelyase Alternative gene PL3 b Y 1.00E-10 pectatelyase Alternative gene PL2 b Y 1.00E-10 pectinmethylesterase Alternative gene CE8 b Y 1.00E-10 MME Core gene PF01182 a N 1.00E-10 mannanase Core gene GH130 b N 1.00E-10 mannanase Alternative gene GH5 b Y 1.00E-25 mannan mannanase Alternative gene GH26 b Y 1.00E-20 mannosidase Alternative gene GH2 b Y 1.00E-50 mannosidase Alternative gene GH5 b Y 1.00E-50 mannanase Alternative gene GH134 b N 1.00E-10 mannanase Alternative gene GH164 b N 1.00E-10 MME Core gene PF07221 a N 1.00E-10 Esterase Core gene CE9 b N 1.00E-10 chitinase Alternative gene GH18 b Y 1.00E-10 chitin chitinase Alternative gene GH19 b Y 1.00E-10

chitinase Alternative gene GH75 b N 1.00E-10 chitinase Alternative gene GH80 b N 1.00E-10 acetylglucosaminidase Alternative gene GH18 b Y 1.00E-30 acetylglucosaminidase Alternative gene GH73 b Y 1.00E-10 acetylglucosaminidase Alternative gene GH84 b Y 1.00E-10 acetylglucosaminidase Alternative gene GH20 b Y 1.00E-10 acetylglucosaminidase Alternative gene GH3 b Y 1.00E-50 chitosanase Alternative gene GH46 b Y 1.00E-10 chitosanase Alternative gene GH8 b Y 1.00E-150 MME Core gene PF13561 a Y 1.00E-40 alginatelyase Alternative gene PL7 b Y 1.00E-10 alginatelyase Alternative gene PL6 b Y 1.00E-10 alginate alginatelyase Alternative gene PL5 b Y 1.00E-10 alginatelyase Alternative gene PL31 b Y 1.00E-50 alginatelyase Alternative gene PL17 b N 1.00E-10 alginatelyase Alternative gene PL34 b N 1.00E-10 alginatelyase Alternative gene PL15 b Y 1.00E-10

Supplementary material 2：Information of bacterial genomes that had predictedagar PUL

Genome name Phylum GCA-number Streptomyces coelicolor A3(2)1 Actinobacteria GCA_000203835.1 Sphingomonas sp. MCT13 2 Alphaproteobacteria GCA_001721295.1 Bacteroidetes/Chlorobi Algibacter lectus3 GCA_000764755.1 group Bacteroidetes/Chlorobi Algibacter lectus3 GCA_900112395.1 group Bacteroidetes/Chlorobi Aquimarina agarilytica ZC14 GCA_000255455.1 group Bacteroidetes/Chlorobi Aquimarina latercula DSM 20415 GCA_000430645.1 group Bacteroidetes/Chlorobi Aquimarina sp. RZW4-3-26 GCA_001632745.1 group Bacteroidetes/Chlorobi Cellulophaga algicola DSM 142377 GCA_000186265.1 group Bacteroidetes/Chlorobi Cellulophaga baltica8 GCA_900102165.1 group Bacteroidetes/Chlorobi Cellulophaga baltica NN0160388 GCA_000477035.2 group Bacteroidetes/Chlorobi Cellulophaga fucicola8 GCA_900119145.1 group Bacteroidetes/Chlorobi Cellulophaga lytica8 GCA_000750195.1 group Bacteroidetes/Chlorobi Cellulophaga lytica8 GCA_001941605.1 group Bacteroidetes/Chlorobi Cellulophaga lytica DSM 7489 9 GCA_000190595.1 group Bacteroidetes/Chlorobi Cellulophaga sp. E6(2014)10 GCA_000764435.1 group Bacteroidetes/Chlorobi Echinicola pacifica DSM 1983611 GCA_000373245.1 group Bacteroidetes/Chlorobi Flammeovirga pacifica12 GCA_000807855.2 group Bacteroidetes/Chlorobi Flammeovirga sp. MY0413 GCA_001682195.1 group Bacteroidetes/Chlorobi Flavobacterium flevense14 GCA_900142775.1 group Bacteroidetes/Chlorobi Formosa agariphila KMM 390115 GCA_000723205.1 group Bacteroidetes/Chlorobi Formosa haliotis16 GCA_001685485.1 group Bacteroidetes/Chlorobi Maribacter aquivivus17 GCA_900142175.1 group Bacteroidetes/Chlorobi Ochrovirga pacifica18 GCA_000220525.2 group Bacteroidetes/Chlorobi Persicobacter sp. JZB0919 GCA_001308105.1 group Bacteroidetes/Chlorobi Polaribacter reichenbachii20 GCA_001975665.1 group Bacteroidetes/Chlorobi Polaribacter reichenbachii20 GCA_001680875.1 group Bacteroidetes/Chlorobi Pseudozobellia thermophila21 GCA_900141855.1 group Bacteroidetes/Chlorobi Reichenbachiella agariperforans22 GCA_900142205.1 group Saccharicrinis fermentans DSM Bacteroidetes/Chlorobi GCA_000517085.1 9555 = JCM 211422324 group Bacteroidetes/Chlorobi Tamlana agarivorans25 GCA_001642835.1 group Bacteroidetes/Chlorobi Tamlana sp. s12 26 GCA_001672305.1 group Bacteroidetes/Chlorobi Zobellia galactanivorans27 GCA_000973105.1 group Bacteroidetes/Chlorobi Zobellia uliginosa28 GCA_000744555.1 group Bacteroidetes/Chlorobi Wenyingzhuangia fucanilytica29 GCA_ 001697185.1 group Bacteroidetes/Chlorobi Aquimarina aggregata6 GCA_ 001632745.1 group Bacteroidetes/Chlorobi Echinicola strongylocentroti30 GCA_ 003260975.1 group Bacteroidetes/Chlorobi Labilibacter sp. CG5131 GCA_ 005877885.1 group Bacteroidetes/Chlorobi Reichenbachiella versicolor32 GCA_ 003171675.1 group

Bacteroidetes/Chlorobi Sediminitomix flava33 GCA_003149185.1 group

Bacteroidetes/Chlorobi Algoriphagus chordae34 GCA_ 003254055.1 group Cellvibrio sp. OA-200735 Gammaproteobacteria GCA_000953825.1 Cellvibrio sp. Pealriver36 Gammaproteobacteria GCA_001183545.1 Gayadomonas joobiniege G737 Gammaproteobacteria GCA_000300815.1 Gilvimarinus agarilyticus38 Gammaproteobacteria GCA_000832015.1 Gilvimarinus chinensis DSM 1966739 Gammaproteobacteria GCA_000377745.1 Gilvimarinus polysaccharolyticus40 Gammaproteobacteria GCA_001187555.1 Marinimicrobium agarilyticum DSM Gammaproteobacteria GCA_000423345.1 16975 41 Microbulbifer agarilyticus S89 42 Gammaproteobacteria GCA_000220505.2 Microbulbifer sp. HZ1143 Gammaproteobacteria GCA_000708675.1 Microbulbifer thermotolerans44 Gammaproteobacteria GCA_001617625.1 Microbulbifer thermotolerans45 Gammaproteobacteria GCA_900112305.1 Paraglaciecola sp. S6646 Gammaproteobacteria GCA_001565895.1 Pseudoalteromonas Gammaproteobacteria GCA_001662245.1 mariniglutinosa47 Saccharophagus degradans 2-40 48 Gammaproteobacteria GCA_000013665.1 Simiduia agarivorans49 Gammaproteobacteria GCA_000305785.2 Simiduia agarivorans49 Gammaproteobacteria GCA_000420285.1 Vibrio sp. EJY3 50 Gammaproteobacteria GCA_000241385.1 Shewanella pacifica51 Gammaproteobacteria GCA_ 002075795.1 Steroidobacter agariperforans52 Gammaproteobacteria GCA_ 004138335.1 Agaribacterium haliotis53 Gammaproteobacteria GCA_002312815 Alteromonadaceae bacterium ALS Gammaproteobacteria GCA_ 003610775.1 8154

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