Discovering hidden biodiversity: the use of complementary monitoring of fish diet based on DNA barcoding in freshwater ecosystems Hyunbin Jo1, Marc Ventura2, Nicolas Vidal3,4, Jeong-Soo Gim1, Teresa Buchaca2, Leon A. Barmuta5, Erik Jeppesen3,4 & Gea-Jae Joo1 1Department of Integrated Biological Science, Pusan National University, Busan 46241, South Korea 2Centre for Advanced Studies of Blanes, Spanish National Research Council (CEAB-CSIC), 17300 Blanes, Catalonia, Spain 3Department of Bioscience, Aarhus University, Vejlsøvej 25, 8600 Silkeborg, Denmark 4Sino-Danish Centre for Education and Research (SDC), Beijing, China 5School of Zoology, University of Tasmania, Private Bag 5, Hobart, Tasmania 7000, Australia Keywords Abstract DNA barcoding, fish diet, monitoring tool, shallow lakes. Ecological monitoring contributes to the understanding of complex ecosystem functions. The diets of fish reflect the surrounding environment and habitats Correspondence and may, therefore, act as useful integrating indicators of environmental status. Gea-Jae Joo, Department of Integrated It is, however, often difficult to visually identify items in gut contents to species Biological Science, Pusan National University, level due to digestion of soft-bodied prey beyond visual recognition, but new Busandaehak-ro 63 beon-gil, Geumjeong-gu, tools rendering this possible are now becoming available. We used a molecular Busan 46241, South Korea. approach to determine the species identities of consumed diet items of an Tel: +82-51-510-2258; Fax: +82-51-583-0172; introduced generalist feeder, brown trout (Salmo trutta), in 10 Tasmanian lakes E-mail:
[email protected] and compared the results with those obtained from visual quantification of stomach contents. We obtained 44 unique taxa (OTUs) belonging to five phyla, Fudning Information including seven classes, using the barcode of life approach from cytochrome The project was funded by The Korea oxidase I (COI).