PPIE Antibody Cat. No.: 29-364
PPIE Antibody
Antibody used in WB on Human K562 cells at 1.25 ug/ml.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Dog, Human
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human PPIE.
TESTED APPLICATIONS: ELISA, IHC, WB
PPIE antibody can be used for detection of PPIE by ELISA at 1:62500. PPIE antibody can be APPLICATIONS: used for detection of PPIE by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
POSITIVE CONTROL: 1) Cat. No. 1204 - K562 Cell Lysate
PREDICTED MOLECULAR 33 kDa, 33 kDa, 26 kDa WEIGHT:
September 30, 2021 1 https://www.prosci-inc.com/ppie-antibody-29-364.html Properties
PURIFICATION: Antibody is purified by protein A chromatography method.
CLONALITY: Polyclonal
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.
CONCENTRATION: batch dependent
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PPIE STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Additional Info
OFFICIAL SYMBOL: PPIE
ALTERNATE NAMES: PPIE, CYP33, CYP-33
ACCESSION NO.: NP_006103
PROTEIN GI NO.: 5174637
GENE ID: 10450
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
PPIE is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities and also exhibit RNA-binding activity. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.The protein encoded BACKGROUND: by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities and also exhibit RNA-binding activity. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.
REFERENCES: 1) Fair, K., (2001) Mol. Cell. Biol. 21 (10), 3589-3597.
ANTIBODIES FOR RESEARCH USE ONLY.
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