PPIE Antibody Cat. No.: 29-364

PPIE Antibody

Antibody used in WB on Human K562 cells at 1.25 ug/ml.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Dog, Human

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human PPIE.

TESTED APPLICATIONS: ELISA, IHC, WB

PPIE antibody can be used for detection of PPIE by ELISA at 1:62500. PPIE antibody can be APPLICATIONS: used for detection of PPIE by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) Cat. No. 1204 - K562 Cell Lysate

PREDICTED MOLECULAR 33 kDa, 33 kDa, 26 kDa WEIGHT:

September 30, 2021 1 https://www.prosci-inc.com/ppie-antibody-29-364.html Properties

PURIFICATION: Antibody is purified by protein A chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store PPIE STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: PPIE

ALTERNATE NAMES: PPIE, CYP33, CYP-33

ACCESSION NO.: NP_006103

PROTEIN GI NO.: 5174637

GENE ID: 10450

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

PPIE is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and activities and also exhibit RNA-binding activity. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.The protein encoded BACKGROUND: by this is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities and also exhibit RNA-binding activity. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.

REFERENCES: 1) Fair, K., (2001) Mol. Cell. Biol. 21 (10), 3589-3597.

ANTIBODIES FOR RESEARCH USE ONLY.

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