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FOR IN VITRO RESEARCH USE ONLY Europe: [email protected] NOT FOR USE IN HUMANS OR ANIMALS China: [email protected] ZNF238 Polyclonal An body Catalog number: 12714-1-AP Background Size: 81 μg/150 μl ZNF238 is a member of the BTB/POZ-ZF protein family, which involve in Source: Rabbit development and cancer forma on, for example BCL-6, PLZF, and HIC-1. It's a Isotype: IgG transcrip onal repressor involve in myogenesis and brain development. By directly Synonyms: repressing the expression of two skeletal myogenesis inhibitors, ID2 and ID3, ZNF238; 58 kDa repressor ZNF238 plays a key role in myogenesis. It can control cell division of progenitor cells protein, C2H2 171, RP58, TAZ 1, and regula ng the survival of postmito c cor cal neurons. Besides, ZNF238 involves TAZ1, Transcrip onal repressor in the organiza on of nuclear chromosomes, for its specific binding to the consensus RP58, ZBTB18, ZFP238, zinc DNA sequence that contains the E box core, and recrui ng chroma n remodeling finger protein 238, Zinc finger mul portein complex. ZNF238 proteins has apparent molecular masses of 60 and 48 protein C2H2 171, ZNF238 kD. Specific binding is found for a 60-kDa band which corresponds to the full length of RP58 protein. In addi on, a 48-kDa band, thought to be the truncated form 2 is detected (PMID: 9756912).
Applications Tested applica ons: ELISA, WB, IP, IHC Cited applica ons: IHC Species specificity: Human,Mouse,Rat; other species not tested. IP result of an -ZNF238 (12714-1-AP Cited species: Mouse for IP and Detec on) with mouse cerebellum ssue. Calculated ZNF238 MW: 531aa,59 kDa Observed ZNF238 MW: 48 kDa Posi ve WB detected in Mouse brain ssue, mouse heart ssue, mouse kidney ssue Posi ve IP detected in Mouse cerebellum ssue Posi ve IHC detected in Mouse brain ssue Recommended dilu on: WB: 1:500-1:5000 IP: 1:500-1:5000 mouse brain ssue were subjected to IHC: 1:20-1:200 SDS PAGE followed by western blot Applica on key: WB = Western blo ng, IHC = Immunohistochemistry, IF = Immunofluorescence, IP = Immunoprecipita on with 12714-1-AP(ZNF238 an body) at dilu on of 1:1000 Immunogen information Immunogen: Ag3406 GenBank accession number: BC036677 Gene ID (NCBI): 10472 Full name: Zinc finger protein 238
Product information Immunohistochemistry of paraffin- Purifica on method: An gen affinity purifica on embedded mouse brain ssue slide Storage: PBS with 0.1% sodium azide and 50% glycerol pH using 12714-1-AP( ZNF238 An body) 7.3. Store at -20oC. at dilu on of 1:50 (under 40x lens)