bioRxiv preprint doi: https://doi.org/10.1101/777334; this version posted September 20, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. Body et al. 1 Submitted to: Frontiers in Physiology 2 3 Manipulation of plant primary metabolism by leaf-mining larvae 4 in the race against leaf senescence 5 6 Mélanie J.A. Body1,2, Jérôme Casas1, and David Giron1* 7 8 (1) Institut de Recherche sur la Biologie de l’Insecte, UMR 7261, CNRS/Université François- 9 Rabelais de Tours, Parc Grandmont, 37200 Tours, France 10 11 (2) Present address: Department of Environmental Sciences, Bowman-Oddy Laboratories, 12 2801 West Bancroft Street, University of Toledo, Toledo, Ohio 43606, United States of 13 America 14 15 * Corresponding author 16 David Giron 17 Address: Institut de Recherche sur la Biologie de l’Insecte, UMR 7261, CNRS/Université 18 François-Rabelais de Tours, Parc Grandmont, 37200 Tours, France 19 Email:
[email protected] 20 Phone number: +33 2 47 36 73 49 21 22 Running head: Plant nutrient alteration by a leafminer 23 24 Conflict of interest 25 26 The authors declare that there is no conflict of interest. 27 28 Author Contributions 29 30 MJAB and DG designed the overall study, and set up the analytical protocols for total 31 sugars, starches, and protein-bound and free amino acid analyses. MJAB carried out the 32 experiments and analyzed the data, supervised by DG.