R2A Agar Reported Improved Re Improved Agar Reported R2A

Revision: 3 Effective Date: 3/27/2019 Appearance: Dehydrated Specifications Control Quality 3. 2. 1. Procedure Test 4. 3. 2. 1. Preparation toSDS Refer Precaution specifications. performance to meet required as supplemented and/or adjusted be may Formula 25 at 0.2 ± 7.2 Final pH: *Equivalent 0.024 to Magnesiumg/L Sulfate Anhydrous Agar Pyruvate Sodium Sulfate Magnesium Phosphate Dipotassium Starch Soluble Dextrose Extract Yeast Casein of Acid Hydrolysate Tissue Animal of Digest Enzymatic of Digest Enzymatic Typical Formulation plate counts. heterotrophic for filter methods and membrane water systems. drinking Agar Methods or Agar Extract Yeast Glucose Tryptone with compared water. When fast of growth chlorine and stressed of growth the stimulates time, incubation longer and temperature incubation withlower a in and combination nutrient medium, is and Geldreich and byReasoner was developed Agar R2A Description humans. in conditions or other disease of diagnosis and Agar R2A Use Intended Sheet Specification Technical

Maintain proper humidity during prolonged incubation prolonged during humidity proper Maintain vary.will filter technique the membrane for to be sample test of volume plate. The or pour per spread dilution or sample of 1exceed mL not Do filter method. or membrane pour plate, plate, spread the by dilutions and sample test Plate the count. plate heterotrophic for dilutions test Prepare 45 toCool minutes. 15 for 121°C at Autoclave medium. the dissolve completely to minute one boil for and agitation frequent with Heat water. purified inliter of one medium the g 18.2 of Suspend c

onforms onforms recommended recommended

(Glucose)

is used for the enumeration and cultivation of bacteria from potable water potable bacteria from of cultivation and the enumeration for is used

- ) - treated in found bacteria or stressed growing slow suppress and may growing bacteria, to

, 50°C.

R2A Agar reported improved re improved Agar reported R2A

uropean Pharmacopoeia r Pharmacopoeia uropean

Heptahydrate Casein

in the European Pharmacopeia Pharmacopeia European the in

R2A Agar is recommended in standard methods for pour plate, spread plate, plate, plate, spread pour for methods standard in is recommended AgarR2A C

Powder is homogeneous, free flowing and light beige to beige. beige. to beige light and flowing free homogeneous, is Powder

R2A AgarR2A(NCM0076)

0.05 15 g/L0.25 g/L 0.25

0.3 g/L 0.3 g/L 0.3 g/L 0.5 g/L 0.5 g/L 0.5 g/L 0.5 .0 *

equirements

g/L g/L -

tolerant bacteria. tolerant covery of stress and chlorine stress of covery

as a separate monograph a separate as

for bacterial plate counts of treated potable water potable treated of plate counts bacterial for

. .

R2A Agar is not intended for use in the the in use for intended is not Agar R2A

Nutritionally rich Nutritionally

Plate Count Agar ( Plate Count - tolerant bacteria from from bacteria tolerant .

R2A Agar is a low is a Agar R2A media support the support the media

in a laboratory setting a laboratory in Standard

filtered filtered

Revision: 3 Effective Date: 3/27/2019 3. 2. 2. 1. References closed. tightly container by keeping at environment humidity low in a container d Store Storage 4. 3. 2. 1. Procedure the of Limitations directed. as stored when intact container the from changed has medium the or if flowing, not free is it if discarded be should medium dehydrated The on the container. stamped date toexpiration Refer Expiration time. of length and temperature units (CF forming colony as Report counts dilution. appropriate the of theby reciprocal per plate colonies of number average the sample of multiplying by mL per count bacterial Compute filter method. membrane the using when 30 demonstrating pour plates or on spread colonies Count Results testing. qualitycontrol used thatfor beshould minimum the listed are organisms The at growth for examined Response: Cultural Expected Appearance: Prepared Sheet Specification Technical Pseudomonas aeruginosa aeruginosa Pseudomonas subtilis Bacillus hydrophila Aeromonas aureus Staphylococcus coli Escherichia faecalis Enterococcus

79th Meeting, Paper No. Q122. No. Paper Meeting, 79th Microbiology for Society American the of the Meeting Annual of Abstracts water systems. drinking Nagy. A. L. and A. Justice, A. C. J., Kelly, water. Journal drinking Staley. and T. A. Mills, A. Vik, E. L., Fiksdal, N7. No. Paper Meeting, 79th Microbiology bacter Geldreich. E. E.and D.J., Reasoner, 9 Pharmacopoeia European bacteria. Incub (44 shock heat bythe compromised be may bacteria stressed of recovery because discouraged is method plate the pour of Use R2A medium. this on grow to orfail poorly grow that encountered be strainsmay some requirements, nutritional varying Due to ehydrated culture media culture ehydrated

Agar is intended for use only with treated potable water. potable treated with only use for intended is Agar ation time longer than indicated above may be necessary to recover additional slow additional recover to necessary be may above indicated longer than time ation ia from potable water. Abstracts of the Annual Meeting of the American Society for Society for the American of Meeting Annual the of Abstracts water. potable from ia

Microorganism

ATCC® ATCC® ATCC® 25922 ATCC®

≤ ATCC® 29212 ATCC® ATCC® 7966 ATCC®

Prepared medium is clear to slightly hazy and light beige. light and hazy slightly is clear to medium Prepared 72 hours incubation. hours 72 ATCC® 25923 ATCC® 6633

AWWA. AWWA. ATCC® 9027 ATCC®

Cultural response on R2A Agar incubated aerobically at at aerobically incubated Agar R2A on response Cultural at th

Edition (2017) (2017) Edition 2

74 30

:313

°C -

1979. A new medium for the enumeration and subculture of of subculture and the enumeration for new Amedium 1979. U) per mL and report variables of incubation such as as such incubation of variables and report mL per U) away from direct sunlight. direct sunlight. from away the same storage temperature. Protect from moisture and light moisture from Protect storage temperature. the same - ° 318. 318. C) and low oxygen tension that are part of the procedure of part are that tension oxygen low and C)

1983. Predominance of chlorine tolerant bacteria in in bacteria tolerant chlorine of Predominance 1983.

Approx. Inoculum Inoculum Approx. 1982. Non 1982.

10 10 10 10 10 10 (CFU) original color. Expiry applies to medium in its to medium applies Expiry color. original ------100 100 100 100 100 100 - -

standard methods for enumerating bacteria in bacteria enumerating for standard methods 300 colonies 300

Once opened and recapped, place place recapped, and opened Once

per plate or20 plate per Expected Results Expected 70 70 70 70 70 70

------

200% recovery 200% recovery 200% recovery 200% recovery 200% recovery 200% recovery 200%

30 -

200 colonies 200 colonies - 35 - growing growing ° C and and C

Revision: 3 Effective Date: 3/27/2019 7. 6. 5. 4. Sheet Specification Technical

enumeration from aquatic environments. Appl. Microbiol. Microbiol. Appl. environments. aquatic from enumeration S.and A., D. Wu Klein, C.H.Lee. and A., A. VanSoestberger, D.C. Association, Washington, Health Public American ed. 23rd wastewater, water and of (eds.). D. Eaton A. and S. Clesceri, L. E., A. Greenberg, Journal AWWA. systems. water distribution in bacteria enumerating for techniques Mea ns, E. G., L. Hanami, H. F. Ridgway, and B. H. Olson. H.Olson. B. and H.F.Ridgway, L. Hanami, E. G., ns, . 1974. Stress: a factor to be considered in heterotrophic microorganisms microorganisms heterotrophic in be considered to a factor Stress: 1974. 1969. Pour plates or streak plates? Appl. Microbiol. Microbiol. Appl. plates? orstreak plates Pour 1969. 2017. 2017. 27 1981. Evaluating mediums and plating mediums Evaluating 1981. :429. :429.

Standard methods for the examination the examination for methods Standard

53 :585

- 590. 590. 18 :1092. :1092.