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(12) United States Patent (10) Patent No.: US 9,157,058 B2 Dalla-Betta Et Al

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(12) United States Patent (10) Patent No.: US 9,157,058 B2 Dalla-Betta Et Al US009 157058E32 (12) United States Patent (10) Patent No.: US 9,157,058 B2 Dalla-Betta et al. (45) Date of Patent: Oct. 13, 2015 (54) METHOD AND APPARATUS FOR GROWING (56) References Cited MICROBIAL CULTURES THAT REQUIRE GASEOUSELECTRON DONORS, ELECTRON U.S. PATENT DOCUMENTS ACCEPTORS, CARBON SOURCES, OR 4,279,754 A 7, 1981 Pollock OTHER NUTRENTS 4,596,778 A 6, 1986 Hitzman 4,760,027 A 7, 1988 Sublette (71) Applicant: Kiverdi, Inc., Berkeley, CA (US) 4,859,588 A 8, 1989 Sublette 5,077,208 A 12/1991 Sublette 5,173,429 A 12/1992 Gaddy et al. (72) Inventors: Peter Dalla-Betta, Hayward, CA (US); 5,593,886 A 1/1997 Gaddy John S. Reed, Berkeley, CA (US) 5,616,288 A * 4/1997 McDonald ...................... 261/76 5,645,726 A 7, 1997 Pollock (73) Assignee: Kiverdi, Inc., Berkeley, CA (US) 5,650,070 A 7, 1997 Pollock 5,807,722 A 9/1998 Gaddy (*) Notice: Subject to any disclaimer, the term of this 5,821,111 A 10/1998 Grady et al. patent is extended or adjusted under 35 5,914,441 A 6/1999 Hunter et al. U.S.C. 154(b) by 0 days. (Continued) (21) Appl. No.: 13/715,430 FOREIGN PATENT DOCUMENTS (22) Filed: Dec. 14, 2012 EP O 131220 A2 1, 1985 EP O227774 B1 9, 1990 (65) Prior Publication Data (Continued) US 2013/O189763 A1 Jul. 25, 2013 OTHER PUBLICATIONS International Search Report and Written Opinion mailed Jun. 14. Related U.S. Application Data 2013 for Application No. PCT/US2012/069830. (60) Provisional application No. 61/570,773, filed on Dec. (Continued) 14, 2011. Primary Examiner —Nathan Bowers (51) Int. C. (74) Attorney, Agent, or Firm — Jill A. Jacobson CI2M I/00 (2006.01) CI2M 3/00 (2006.01) (57) ABSTRACT CI2N L/20 (2006.01) Compositions and methods and apparatus for growth and (52) U.S. C. maintenance of microorganisms and/or bioprocesses using CPC ................. CI2N 1/20 (2013.01); CI2M 29/02 one or more gases as electron donors, electron acceptors, (2013.01); C12M 29/18 (2013.01); C12M 43/04 carbon sources, or other nutrients, and for a bioprocess that (2013.01) converts hydrogen and carbon dioxide, or syngas, or producer (58) Field of Classification Search gas into lipid products, bio-based oils, or other biochemical CPC ...... C12M 23/40; C12M 27/00; C12M 27/18: products. C12M 27/24 See application file for complete search history. 23 Claims, 12 Drawing Sheets inlet waive controited by in-line sensors Press re-relief valve gas feed marifold Gas feed port media Siface SSS 2 Cell down-tube Liquid outlet liquid circulation pump Effluent tube US 9,157,058 B2 Page 2 (56) References Cited Bergmann et al., Assimilation of carbon dioxide by hydrogen bacte ria. J Biol Chem. Jan. 1958:230(1):13-24. U.S. PATENT DOCUMENTS Bernstein et al., Climate Change 2007: Synthesis Report. IPCC Ple nary XXVII. Valencia, Spain. Nov. 12-17, 2007. 51 pages. 5,989,513 A 11, 1999 Rai 6,043,022 A 3/2000 Lueking et al. Bilal et al., Thermo-electrochemical reduction of sulfate to sulfide 6,340,581 B1 1/2002 Gaddy using a graphite cathode. Journal of Applied Electrochemistry. Oct. 6,667,171 B2 12/2003 Bayless et al. 1998:28(10): 1073-1081. 6,972,188 B2 12/2005 Maekawa et al. Bird et al., TCERs or ICERs? 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