Volume 20, issue 3, pages 106–117 30 September 2020 https://doi.org/10.33493/scivis.20.03.02 RESEARCH ARTICLE DNA barcoding reveals a new country record for three species of frogs (Amphibia: Anura) from India Samuel Lalronunga1,2*, Vanramliana3, C. Lalrinchhana4, Vanlalhrima2, Vanlalhriatzuala Sailo2, Lalnunhlua2, Lalkhawngaiha Sailo2, Isaac Zosangliana1,2, K. Lalhmangaiha1,2, Esther Lalhmingliani1,2 1Systematics and Toxicology Laboratory, Department of Zoology, Mizoram University, Aizawl 796004, Mizoram, India 2Biodiversity and Nature Conservation Network, Aizawl 796004, Mizoram, India 3Department of Zoology, Pachhunga University College, Aizawl 796001, Mizoram, India 4Holy Child School, Nalkata, Dhalai, Tripura 799263, India We present a first record for three species of frogs, viz. Amolops indoburmanensis, Received 25 May 2020 Accepted 12 August 2020 Euphlictys kalisgraminensis and Polypedates braueri in India based on DNA barcoding data. We also discussed on the records of Amolops spp. in Mizoram and *For correspondence:
[email protected] delisted all previous recorded species, viz. A. afghanus, A. kaulbacki and A. marmoratus from the amphibian fauna of Mizoram until further research confirmed Contact us:
[email protected] their presence, since the data were not sufficient enough for the confirmation of their occurrence and are likely based on misidentification of A. indoburmanensis. Key words: Biodiversity hotspot, conservation, 16S rRNA, northeast India, genetic distance. Introduction Four of the thirty six global biodiversity hotspots amphibian species identification and detection of partly fall within the territory of India, viz. Western cryptic species.11-14 Ghats, Indo-Burma, Himalaya and Sundaland.1-3 India Most part of northeast India falls within the Indo- harbours high species diversity and endemism in Burma and Himalaya biodiversity hotspots.