39Th International Symposium on High-Performance Liquid Phase

HPLC 2013 39th International Symposium on High Performance Liquid Phase Separations and Related Techniques

RAI CONGRESS CENTRE THE NETHERLANDS 16-20 JUNE 2013

Final Program

Contents

Welcome Message ...... 3 Sponsors ...... 4 Media Partners ...... 7 Supporting Organizations ...... 8 Committees ...... 9 History and Future Meetings ...... 10 Awards ...... 12 Short Courses ...... 14 Lecture Program ...... 15 Lunch & Breaks Scheme/Special Events ...... 16 List of Posters ...... 40 Vendor seminars ...... 147 Symposium proceedings ...... 151 Information for Speakers and Poster Presenters ...... 152 Exhibition ...... 153 Exhibitors ...... 155 Exhibition Area ...... 162 Social Events ...... 165 General Information ...... 166

Welcome Message

Welcome to Amsterdam and the 39th International Symposium on High-Performance Liquid-Phase Separations and Related Techniques.

HPLC2013 Amsterdam is the third meeting in this series that is held in The Netherlands – after 1987 (Amsterdam) and 2001 (Maastricht). Not bad for a country with mostly baseline and very few peaks. Throughout the last fifty or sixty years many chromatographers gathered in our country, starting with van Deemter and following on with the likes of Carel Cramers, Udo Brinkman, and the brilliant Hans Poppe, as well as imported talent, such as Joseph Huber and Roland Frei. Like painters in the 17th century they all found their way to Amsterdam. Amsterdam is one of the natural hubs of the old and the new Europe. Odds are that you found it easy to get here. In fact, odds are that you have been here before and that you will come back. Although us Dutch can be impolite and ill-mannered (not only the cab drivers), we do appreciate you being here. To demonstrate our hospitality, just about everyone in Amsterdam can communicate with you in English. We offer you a generous amount of separation science this week. We have three hours of lectures on a typical morning or afternoon, plus posters, technical seminars and tutorials. We offer you generous amounts of coffee, food, and drinks (on four occasions during the week). We offer you free internet at the conference and Petra’s posse is offering all the help you need. And, yes, we are frugal! I have great memories of the incredible HPLC1987 Amsterdam. Our aim is to give you a week that you will treasure until at least 2040. On behalf of the entire team of HPLC2013 Amsterdam, welcome! Peter Schoenmakers

Sponsors

The organization of the HPLC symposium would not have been possible without the generous and invaluable support of the following companies and organizations., Platinum Sponsors

Agilent Technologies Agilent Technologies is a global provider of leading measurement technology for evaluating the environment, foods, pharmaceuticals, industrial processes, and biological samples for over 40 years and has 19,0000 employees worldwide. A leader in GC and GC/MS, Agilent also makes some of the world's bestselling liquid chromatography and LC/MS systems and holds leadership positions in consumables, software, genomics, reagents, lab automation, support and compliance services.

Shimadzu Europe Shimadzu is one of the worldwide leading manufacturers of analytical instrumentation for consumer protection, product and environmental safety. Latest developments include the Nexera X2 UHPLC system, which targets routine analysis and applications in highly regulated environments, the LCMS-8040, the world’s fastest LCMS/MS tandem mass spectrometer, the Perfinity iDP* (integrated Digestion Platform) online enzyme digestion HPLC system and the Nexera MP, which is perfect as a Front End system for LC/MS analysis conducted in pharmacokinetics and synthesis stages in drug detection processes. Its new SIL-30ACMP Multiplate Autosampler provides industry-leading features.

Thermo Scientific Thermo Fisher Scientific Inc. is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. We serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. Our three premier brands are Thermo Scientific, Fisher Scientific and Unity™ Lab Services, which offer a unique combination of innovative technologies, convenient purchasing options and a single solution for laboratory operations management. Our products and services help our customers solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity.

Waters Corporation Waters Corporation, the premium brand in the analytical instruments industry, creates business advantages for laboratory-dependent organizations by delivering practical and sustainable scientific innovation to enable significant advancements in healthcare delivery, environmental management, food safety, and water quality worldwide. Bringing keen understanding and deep experience to those responsible for laboratory infrastructure and performance, Waters helps customers make profound discoveries, optimize laboratory operations, deliver product performance, and ensure regulatory compliance. Pioneering a connected portfolio of separations and analytical science, laboratory informatics, mass spectrometry, as well as thermal analysis, Waters’ technology breakthroughs and laboratory solutions provide an enduring platform for customer success.

Gold Sponsors

AB SCIEX

AB SCIEX is a global leader in the development of best-in-class technologies that help answer complex scientific challenges and improve our world. We create scientific instrumentation, software and services for the life science, clinical research and industrial markets, empowering you with technologies that are used in drug discovery and manufacturing, food and environmental safety, and clinical research.

Merck Millipore

Molnár-Institute for applied chromatography

For over 30 years, Molnár-Institute has delivered chromatography modeling solutions to the pharmaceutical, chemical, food, biotech and research industries. Our UHPLC modeling software DryLab® is used worldwide and offers an intuitive and visual solution for the creation of fast, robust and high quality methods. With its comprehensive resolution and robustness mapping abilities, DryLab® enables scientists to easily determine their optimal working space, and to gain unprecedented insight into the effects of various parameters on the resolution and selectivity of the separation. We look forward to meeting you at our booth E18!

Sigma-Aldrich International Sigma-Aldrich is a leading Life Science and High Technology company committed to enabling Science to improve the quality of Life. Supelco, Fluka, Cerilliant and RTC are the analytical brands of Sigma-Aldrich. Supelco’s innovative chromatography products together with Fluka’s high quality analytical solvent, reagents and standards are used for research and control in Pharmaceutical, Food, Environmental, Clinical and many others areas. Recently Cerilliant and RTC joined the Sigma-Aldrich family to further increase the quality offering of Certified Reference Materials and Proficiency testing. Sigma-Aldrich operates in 40 countries and has over 9,600 employees providing excellent service worldwide. Welcome to visit us in person at Booth A10 during HPLC 2013 in Amsterdam to discuss the latest in particle technology/phase chemistry or let us know how we can make you more successful.

Silver Sponsors

Knauer - Wissenschaftliche Geraetebau Dr. Ing. H. Knauer

KNAUER manufactures UHPLC and HPLC systems for the analytical up to the preparative range of applications. The instruments are used in science, quality control, and test laboratories worldwide. KNAUER is a competent partner for continuous chromatography using SMB and MCSGP technologies. For high resolution analysis or purification of proteins and other biomolecules KNAUER offers perfectly matched biochromatography systems, pressure stable glass columns, and separation media. LC columns, UV/VIS/NIR and RI detection systems, as well as appropriate software complete the choice of high quality LC products. KNAUER also supplies osmometers for chemistry and clinical use. KNAUER is certified ISO 9001/14001

Restek Corporation

Phenomenex

Phenomenex is a global leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories. The company’s core technologies include products for liquid chromatography, gas chromatography, sample preparation, bulk purification chromatographic media, and chromatography accessories and equipment. Phenomenex is responsible for many HPLC innovations over the years, most recently including the award-winning Kinetex® Core-Shell Technology.

Bronze Sponsors

SCAT Europe

Media Partners

Supporting Organizations

CASSS - An International Separation Science Society Arbeitskreis Separation Science Association Francophone des Sciences Séparatives Austrian Society of Analytical Chemistry (ASAC) Czech Chemical Society - Chromatography & Electrophoresis Group Deutschen Gesellschaft für Massenspektrometrie (DGMS) Divisioni di Chimica Analitica Hungarian Society for Separation Sciences Koninklijke Vlaamse Chemische Vereniging (KVCV) KNCV Sectie Analytische Chemie (SAC) Nederlandse Vereniging voor Massaspectrometrie (NVMS) Polska Akademia Nauk (PAN) Sociedad Española de Cromatografía y Técnicas Afines (SECyTA) The Chromatographic Society (ChromSoc)

University of Amsterdam

Committees

Scientific Committee

Permanent members Atilla Felinger (Hungary) John Frenz (USA) Georges Guiochon (USA) Bill Hancock (USA) Gérard Hopfgartner (Switzerland) Barry Karger (USA) Wolfgang Lindner (Austria) Koji Otsuka (Japan) Peter Schoenmakers (The Netherlands) Guowang Xu (China) Ed Yeung (USA)

Ad hoc members Gert Desmet (Belgium) Monika Dittmann (Germany) Paola Dugo (Italy) Emily Hilder (Australia) Michael Lämmerhofer (Germany) David McCalley (UK) Michel Nielen (The Netherlands) J. Michael Ramsey (USA) Marja-Liisa Riekkola (Finland) Ulrich Tallarek (Germany) Chairmen Peter Schoenmakers Wim Kok Local Organizers Tom Aalbers Petra Aarnoutse Peter Verschuren Gabriel Vivó Truyols PCO Conference Administration Organisation Services (CAOS)

History and Future Meetings

The International Symposium on High Performance Liquid Phase Separations (HPLC) is an international forum for the scientific discussion of the methods of high performance liquid chromatography in its various forms, along with the complementary separation techniques such as electrophoresis, electrochromatography, field-flow fractionation, supercritical fluid chromatography and hyphenated techniques, such as LC/MS and CE/MS. In addition, microfluidics, separations on chips, diagnostic systems, and other leading technologies are also considered. This conference originated in 1973. Advances in the field of liquid-phase separations are highlighted at each meeting. The meetings alternate between Europe and the United States with even years being in the US. Since 2008 additional meetings are held in Asia and other continents.

Past and future symposia and chair(s) Year Location Chair(s) 1973 Interlaken, Switzerland Willy Simon 1975 Wilmington, USA Jack J. Kirkland 1977 Salzburg, Austria Joseph F.K. Huber 1979 Boston, USA Barry L. Karger 1981 Avignon, France Georges Guiochon 1982 Cherry Hill, USA Robert Bardford 1983 Baden-Baden, Germany Klaus Peter Hupe 1984 New York City, USA Csaba Horvath 1985 Edinburgh, UK John H. Knox 1986 San Francisco, USA Ronald E. Majors 1987 Amsterdam, The Netherlands Hans Poppe 1988 Washington, USA Georges Guiochon 1989 Stockholm, Sweden Douglas Westerlund 1990 Boston, USA Barry L. Karger 1991 Basel, Switzerland Fritz Erni

Year Location Chair(s) 1992 Baltimore, USA Fred E. Regnier 1993 Hamburg, Germany Klaus K. Unger 1994 Minneapolis, USA Larry D. Bowers and Peter W. Carr 1995 Innsbruck, Austria Wolfgang Lindner 1996 San Francisco, USA William S. Hancock 1997 Birmingham, UK Anthony F. Fell 1998 St. Louis, USA Daniel W. Armstrong 1999 Granada, Spain Emilio Gelpi 2000 Seattle, USA Edward S. Yeung 2001 Maastricht, The Netherland Hans Poppe and Henk Lingeman 2001 Kyoto, Japan Nobuo Tanaka and Shigeru Terabe 2002 Montreal, Canada Irving W. Wainer 2003 Nice, France Antoine M. Siouffi 2004 Philadelphia, USA Mark R. Schure 2005 Stockholm, Sweden Douglas Westerlund 2006 San Francisco, USA John H. Frenz 2007 Gent, Belgium Jacques Crommen and Pat Sandra 2008 Baltimore, USA Georges Guiochon and Steven Jacobson 2008 Kyoto, Japan Koji Otsuka and Nobuo Tanaka 2009 Dresden, Germany Christian Huber 2010 Boston, USA Steven A. Cohen 2011 Budapest, Hungary Attila Felinger 2011 Dalian, China Guowang Xu 2012 Anaheim, USA Frantisek Svec 2013 Amsterdam, The Netherlands Peter Schoenmakers and Wim Kok 2013 Hobart, Australia Paul Haddad and Emily Hilder 2014 New Orleans, USA J. Michael Ramsey 2015 Geneva, Switzerland Gérard Hopfgartner 2016 San Francisco, USA Robert T. Kennedy 2017 Prague, Czech Republic Michal Holčapek and Frantisek Foret

Awards

The Csaba Horváth Young Scientist Award

About the Award The purpose of the Award is to honor the memory of Csaba Horváth and recognize his contributions to HPLC, including his interest in fostering the careers of young people in separations science and engineering. The award includes an invitation to speak at the next HPLC symposium, a grant to support travel to that meeting, and a trophy engraved with the winner's name. The award is sponsored by HPLC, Inc.

About Csaba Horváth Professor Csaba Horváth (1930-2004) was born in Hungary and graduated in chemical engineering from the Budapest Institute of Technology. After receiving his Ph.D. in physical chemistry at the J.W. Goethe University in Frankfurt under the direction of Prof. Halász, he immigrated to the United States in 1963 and started research at the Harvard Medical School. In the following year, Dr. Horváth moved to Yale where he designed and built the first high performance liquid chromatograph to demonstrate the feasibility and potential of HPLC in bioseparation sciences. He chaired the Department of Chemical Engineering at Yale from 1987 to 1993 and was named as Roberto C. Goizueta Professor of Chemical Engineering in 1998. Professor Horváth contributed close to 300 publications to the field of separation sciences and had nine patents. His main topics were all fundamental aspects of separations, including instrumentation, stationary phase designs, and mechanisms of separation processes, as well as their application mainly to biological and biomedical research, especially for the high-resolution separation of proteins and peptides.

Eligibility Criteria All presenters of oral contributions (excepting past winners) who are less than 35 years of age at the time of their lecture are eligible for consideration. Candidates will be required to provide evidence of eligibility (e.g., passport, driver's license).

Selection Process The Scientific Committee selects abstracts for inclusion in the oral program. An Award Jury judges the eligible presentations and chooses a winner. The winner will be announced at the closing ceremony, Thursday, June 20th at 16:40 in the Auditorium.

Finalists The finalists are indicated in the lecture program by the red coloured lecture number. OR67 Michelle Camenzuli OR49 Miroslav Lisa University of Western Sydney, Parramatta, NSW, AU University of Pardubice, Pardubice, CZ OR5 Wim De Malsche OR84 Sandra Pous Torres Vrije Universiteit Brussel, Brussels, BE University of Valencia, Burjassot, ES OR6 Claudia Ernst OR72 Robert-Jan Raterink University of Leipzig, Leipzig, DE Leiden University, Leiden, NL OR34 James Grinias OR88 Roland Reischl University of North Carolina, Chapel Hill, NC, US Paris Lodron University Salzburg, Salzburg, AU OR8 Krisztián Horváth OR40 Elena Uliyanchenko University of Pannonia, Veszprém, HU SABIC, Bergen op Zoom, NL

Agilent Technologies Best-Poster Awards Agilent Technologies is the long-standing sponsor of the poster awards at the HPLC meetings. Also this year, Agilent are generously sponsoring ten awards of 500 € each for the best posters.

Expert Review Gerard Rozing will lead a jury consisting of a large number of recognized international experts on liquid phase separations. The jury will publically nominate up to 30 posters on Wednesday evening. Posters are reviewed based on excellence in terms of three criteria

Inspiration (creativity, novelty, uniqueness, originality) “Transpiration” (experimental execution, completeness of the work) Presentation (overall readability of the poster, visual impression, objectives and conclusions, author's explanations)

In the final round on the last day of the conference, seven of these nominated posters will be selected to receive Agilent Technologies best-poster awards.

Public poll Statistician Martin Lopatka organizes the public vote. All participants (industrial and academic scientists, students and exhibitioners) are urged to vote. The public voting will take place at Poster Headquarters in the exhibition area. Poster HQ is also the home of the expert jury and the place where jurors report for duty. The three highest scoring posters in the public poll will also receive Agilent Technologies best-poster awards.

Qualification To qualify for a poster award you must have declared yourself a candidate upon submitting your abstract. To qualify for the public poll you should have submitted a pdf copy of your poster by June 12th 2013.

Uwe Neue Award (sponsored by Waters) Uwe Neue was an outstanding liquid chromatographer. Two-and-a-half years after he passed away the first award that carries his name will be presented to an industrial scientist who – like Uwe – has made significant contributions to the field of separation science and has strived to see these implemented in commercial products. The award underlines what Uwe Neue has amply demonstrated. Great scientists can make great contributions and have great careers in industry. The award will be presented on Tuesday, June 18th at 8:40 in the Auditorium by Martin Gilar of Waters and Georges Guiochon on behalf of the permanent scientific committee to Dr. J.J. Kirkland, vice president of R&D Technologies, Advanced Materials Technology, for his advancements in chromatography and dedication to separation science.

Short Courses

All short courses take place in parallel on Sunday, June 16, from 9:00 to 16:00 in the RAI Congress Centre, Amsterdam. Short course 1 Sample Preparation in HPLC Lourdes Ramos and María Luz Sanz Dpt. Análisis Intrumental y Química Ambiental, IQOG-CSIC, ES Lecture Hall G104

Short course 2 Comprehensive Two-Dimensional Liquid Chromatography (LC×LC) 1Dwight Stoll and 2Xiaoli Wang 1Gustavus Adolphus College, US; 2Agilent Technologies, Wilmington, DE, US Lecture Hall G105

Short course 3 LC-MS(/MS) Gérard Hopfgartner University of Geneva, CH Lecture Hall G106

Short course 4 Retention Mechanisms in HPLC María Celia García-Álvarez-Coque and José Ramón Torres Lapasió University of Valencia, ES Lecture Hall G107

Lunch and breaks

8:30 – 9:00 Coffee-Tea 10:30 – 11:00 Coffee-Tea break 12:00 – 13:00 Lunch 14:15 – 14:45 Coffee-Tea break

Lecture Program

Sunday, June 16, 2013 16:15 – 19:00

Plenary Session Auditorium

16:15 – 17:00 Opening Ceremony 17:00 – 17:40 PL1 Fight against Cancer Jos Beijnen Slotervaart Hospital, Amsterdam, NL Introduced by Wim Kok 17:40 – 18:10 PL2 Top Ten HPLC Column Myths Ron Majors Wilmington, DE, US

Introduced by Peter Schoenmakers and Laura Bush 18:10 – 18:40 PL3 Forget Everything, Except Mass Spectrometry Michel Nielen Wageningen University and RIKILT, Wageningen, NL Introduced by Gérard Hopfgartner 18:40 – 19:00 PL4 From Pore Level Reconstruction to Morphological Analysis of Eddy Dispersion Stefan Bruns, Tibor Müllner, Ulrich Tallarek Philipps – Universität Marburg, Marburg, DE Introduced by John Frenz 19:00 – 19:10 ChromSoc Awards Presented by Paul Ferguson

19:15 – 21:00 Welcome Reception Sponsored by Shimadzu Corporation

Lunch & Breaks Scheme/Special Events

Monday to Wednesday, June 17 – 19

11:00 – 11:30 Coffee-Tea break / Exhibition & Posters 12:30 – 14:00 Lunch / Exhibition & Posters 16:00 – 17:00 Coffee-Tea break / Exhibition & Posters

Thursday, June 20

11:00 – 11:30 Coffee-Tea break / Exhibition & Posters 12:30 – 13:30 Lunch / Exhibition & Posters 14:30 – 14:50 Coffee-Tea break

Special Events Tuesday, June 18, Auditorium

8:40 – 9:00 Uwe D. Neue Award (sponsored by Waters Corporation) Presented by Martin Gilar and Georges Guiochon Wednesday, June 19, Auditorium

8:40 – 9:00 High-Performance Lindner Chromatography by Michael Lämmerhofer and Barry Karger

Monday, June 17, 2013 9:00 – 11:00

HYPERformance LC ̶ Columns Auditorium Chair: Ulrich Tallarek and Paul Ferguson 9:00 – 9:30 KN1 The van Deemter equation: What Does it Mean 50 Years Later? Fabrice Gritti, Georges Guiochon University of Tennessee, Knoxville, TN, US 9:30 – 10:00 KN2 Monolithic Columns in Thin Layer Format for TLC-MS Separations Frantisek Svec, Alexandros Lamprou, Zhixing Lin, Yongqin Lu LBNL, The Molecular Foundry, Berkeley, CA, US 10:00 – 10:20 OR1 New Developments in Chromatographic Applications for the Enrichment and Separation of Biological Molecules Günther Bonn Institute of Analytical Chemistry and Radiochemistry, Innsbruck, AT 10:20 – 10:40 OR2 Mashmallow Gels: Monolithic Macroporous PDMS Analogue for Versatile Separations Kazuki Nakanishi1, Gen Hayase1, Kazuyoshi Kanamori1, Kei Morisato2 1Kyoto University, Graduate School of Science, Kyoto, JP; 2GL Sciences/Kyoto University, Graduate School of Science, Kyoto, JP 10:40 – 11:00 OR3 HYPERperformance Carbon Phases for Ion Chromatography and Hydrophilic Interaction Liquid Chromatography Charles A. Lucy, M. Farooq Wahab, Mohammed E.A. Ibrahim Department of Chemistry/University of Alberta, Edmonton, Alberta, CA

High-Impact LC ̶ Pharmaceutical Analysis Emerald Chair: Gérard Hopfgartner and Martin Giera 9:00 – 9:30 KN7 Improving Patient Outcome by Strategic Build within the Healthcare Sector Gyorgy Marko-Varga Lund University, Lund, SE 9:30 – 10:00 KN8 State-of-the-art Liquid Chromatography for (Bio)Pharmaceutical Analysis Pat Sandra, Koen Sandra Research Institute for Chromatography, Kortrijk, BE 10:00 – 10:20 OR10 Combining Three HPLC Modes and Polysaccharide-Based Chiral Stationary Phases in One Generic Separation Strategy Debby Mangelings1, Ahmed Younes2, Hasret Ates1, Yvan Vander Heyden1 1Vrije Universiteit Brussel - FABI, Brussels, BE; 2Helwan University, Cairo, EG

10:20 – 10:40 OR11 Enantioselective Ultrahigh-Pressure Liquid Chromatography and Ultrahigh- Performance Supercritical Fluid Chromatography in the Chiral Science Separation Scenario: Ultra-Fast Separations on a New Sub-2-µm Whelk-O 1 Stationary Phase Dorina Kotoni1, Alessia Ciogli1, Marco Pierini1, Jelena Kocergin2, Ted Szczerba3, Harald Ritchie4, Claudio Villani1, Francesco Gasparrini1 1Sapienza University of Rome, Rome, IT; 2Regis Technologies, Morton Grove, Illinois, US; 3Regis Techn., Morton Grove, Illinois, US; 4Thermo Fisher Scientific, Runcorn, GB 10:40 – 11:00 OR12 Identification of Drug Target Spectrum by Monolith Affinity Chromatography and HPLC-Mass Spectrometry Jingwu Kang, Xuepei Zhang Shanghai Institute of Organic Chemistry, CAS, Shanghai, CN

Monday, June 17, 2013 9:00 – 11:10

HPLC-MS ̶ Quantitative LC-MS/Ionization Methods G102-103 Chair: Wilfried Niessen and Koen Sandra 9:00 – 9:20 OR20 Novel Isotope Dimethyl Labeling-Based Methods for Quantitative Proteomics Study with High Accuracy and Precision Yuan Zhou, Yichu Shan, Lihua Zhang, Zhang Yukui Dalian Institute of Chemical Physics, CAS, Dalian, CN 9:20 – 9:40 OR21 LC-MS: A Delivery Platform for Drug Targets and Biomarkers Iulia M. Lazar Virginia Polytechnic Institute and State University, Blacksburg, VA, US 9:40 – 10:00 OR22 Clean-Up Strategies to Overcome the Matrix Effect in the Determination of Polyether Ionophores in Sewage Sludge by Liquid Chromatograpy-Tandem Mass Spectrometry Pol Herrero, Francesc Borrull, Eva Pocurull, Rosa Maria Marcé Universitat Rovira i Virgili, Tarragona, ES 10:00 – 10:30 KN11 Ionization Methods in Liquid Chromatography-Mass Spectrometry Risto Kostiainen University of Helsinki, Faculty of Pharmacy, Helsinki, FI 10:30 – 11:00 KN12 New Ionization Methods for Use in Mass Spectrometry Sarah Trimpin Wayne State University, Detroit, MI, US

Tutorials G104-105

9:30 – 10:10 TU1 The Role of the Column in Modern HPLC/UHPLC Ron Majors Wilmington, DE, US Introduced by Katya Davydova 10:30 – 11:10 TU2 Microfluidic Chemical Separations Devices J. Michael Ramsey University of North Carolina, Chapel Hill, NC, US Introduced by Sebastian Thürmann

Monday, June 17, 2013 11:30 – 12:30

HYPERformance LC ̶ Miniaturization Auditorium Chair: Stephen Jacobson 11:30 – 11:50 OR4 Sample Trapping in Microfluidic LC: Theoretical and Practical Considerations Martin Gilar, Gregory Roman, Jay Johnson, Jim Murphy Waters Corp., Milford, MA, US 11:50 – 12:10 OR5 Reaching Sub-μm Plate Heights in HPLC by Radial Extension of the Support Structures Wim De Malsche1, Jeff Op De Beeck1, Manly Callewaert1, Heidi Ottevaere1, Han Gardeniers2, Gert Desmet1 1Vrije Universiteit Brussel, Brussels, BE; 2UTwente, Enschede, NL 12:10 – 12:30 OR6 Two-Photon Excited Fluorescence Detection in Chip Electrochromatography Claudia Ernst, Reinhild Beyreiss, Detlev Belder University of Leipzig, Institute of Analytical Chemistry, Leipzig, DE

High-Impact LC ̶ Pharmaceutical Analysis Emerald Chair: Gérard Hopfgartner and Martin Giera 11:30 – 11:50 OR13 Applications of Two-Dimensional Liquid Chromatography in Pharmaceutical Analysis C.J. Venkatramani, James Girotti, Larry Wigman, Nik Chetwyn Genentech Inc, South San Francisco, US 11:50 – 12:10 OR14 Method Development and Application of Ultra-Fast Supercritical Fluid Chromatography in Pharmaceutical Analysis Lucie Nováková1, Michal Douša2, Josef Reitmajer2, Petr Solich1 1Charles University in Prague, Faculty of Pharmacy, Hradec Králové, CZ; 2Zentiva a.s., Prague, CZ 12:10 – 12:30 DI1 Discussion on needs for pharmaceutical analysis

HPLC-MS ̶ Quantitative LC-MS G102-103 Chair: Yolanda Pico 11:30 – 12:00 KN13 Reactions of Gadolinium Chelates in Biological Matrices and the Environment Unraveled by Hyphenated Analytical Techniques Uwe Karst, Lena Telgmann, Marvin Birka, Christoph Wehe, Michael Sperling University of Münster, Münster, DE 12:00 – 12:30 KN14 Feasibility of Direct-EI LC-MS as a Tool for Food Safety Applications: Identification and Quantitation of Environmental Contaminants in Milk and Nutritional Drink Achille Cappiello1, Pierangela Palma1, Giorgio Famiglini1, Veronica Termopoli1, Fabiana Capriotti1, Nick Cellar2 1University of Urbino, Urbino, IT; 2Abbott Nutrition, Columbus, OH, US

Tutorial G104-105

11:50 – 12:30 TU3 Ultra High Performance Liquid Chromatography (UHPLC) Monika Dittmann, Agilent Technologies, Waldbronn, DE Introduced by Sandra Pous Torres

Monday, June 17, 2013 14:00 – 16:00

HYPERformance LC ̶ Fundamentals Auditorium Chair: Monika Dittmann and Ivo Nischang 14:00 – 14:30 KN3 A New Procedure for Predictions of Overloaded Profiles in Gradient Elution Torgny Fornstedt1, Dennis Åsberg1, Marek Lesko2, Martin Enmark1, Patrik Forssén1, Jörgen Samuelsson1, Krzysztof Kaczmarski2 1Karlstad University, Karlstad, SE; 2Rzeszow University of Technology, Rzeszów, PL 14:30 – 15:00 KN4 Time Scales in Liquid Chromatography – from Picoseconds to Kiloseconds Attila Felinger University of Pécs, Pécs, HU 15:00 – 15:20 OR7 Highly Efficient Capillary Columns Packed with Sub-2 Micron Superficially Porous Particles James Treadway, James Jorgenson University of North Carolina - Chapel Hill, Chapel Hill, NC, US 15:20 – 15:40 OR8 One Step Further on the Way Toward the Ultimate Stationary Phase: Analysis of Efficiency of Bi-Layer Core-Shell Particles Krisztián Horváth, Péter Hajós University of Pannonia, Veszprém, HU 15:40 – 16:00 OR9 Perfluorinated Adsorbents for UHPLC and Environmental Applications: Geometrical Properties, Wetting Behavior and New Insights into Retention Mechanisms Alberto Cavazzini1, Nicola Marchetti1, Alessia Ciogli2, Francesco Dondi1, Francesco Gasparrini2, Aldo Laganà2, Donatella Capitani3 1University of Ferrara, Ferrara, IT; 2University of Rome, Rome, IT; 3Istituto Metodologie Chimiche CNR Area Ricerca, Monterotondo, Rome, IT

High-Impact LC ̶ Environmental Analysis Emerald Chair: Michael Lämmerhofer and Deirdre Cabooter 14:00 – 14:30 KN9 Analytical Challenges Related to the Elucidation of the Chemical Compounds Taken Part in Atmospheric Aerosol Formation Marja-Liisa Riekkola1, Jose Ruiz-Jimenez1, Jevgeni Parshintsev1, Totti Laitinen1, Kari Hartonen1, Tuukka Petäjä2, Markku Kulmala2 1University of Helsinki, Department of Chemistry, Helsinki, FI; 2University of Helsinki, Department of Physics, Helsinki, FI 14:30 – 15:00 KN10 High Resolution Mass Spectrometry (LC/Q-TOF-MS) for the Identification of Emerging Contaminants in Water Imma Ferrer, Michael Thurman University of Colorado, Boulder, CO, US 15:00 – 15:20 OR15 Miniaturization of Selective Extraction Sorbents for their On-Line Coupling with Capillary Chromatography or their Introduction on Chip Valerie Pichon, Fabien Brothier, Wassim Hadj Ali LSABM, ESPCI -UMPC -CNRS, Paris, FR 15:20 – 15:40 OR16 Development of a Comprehensive Two-Dimensional Nano-/Capillary-LC system with Mass-Spectrometric Detection for the Screening Analysis of Complex Samples Thorsten Teutenberg, Jakob Haun, Juri Leonhardt, Christoph Portner Institut für Energie- und Umwelttechnik e. V., Duisburg, Duisburg, DE 15:40 – 16:00 OR17 Evaluation of Quantitation in Two Dimensional Liquid Chromatography Catherine Rimmer, Mallory Morris, Melissa Phillips, Benjamin Place, Lane Sander National Institute of Standards and Technology, Gaithersburg, MD, US

Monday, June 17, 2013 14:00 – 16:00

HPLC-MS ̶ Microscale LC-MS and CE-MS G102-103 Chair: J. Michael Ramsey and Frantisek Foret 14:00 – 14:20 OR23 Ultrafast Separation on Photonic Crystal Beds Yi Chen Institute of Chemistry, Chinese Academy of Sciences, Beijing, CN 14:20 – 14:40 OR24 Parallel Artificial Liquid Membrane Extraction– Micro-Scale Liquid-Liquid- Liquid Extraction in the 96-Well Format Astrid Gjelstad, Knut Einar Rasmussen, Marthe Petrine Parmer, Stig Pedersen-Bjergaard School of Pharmacy, University of Oslo, Oslo, NO 14:40 – 15:00 OR25 A Microchip Emitter for Solid Phase Extraction – Gradient Elution – Mass Spectrometry Natalia Gasilova, Liang Qiao, Dmitry Momotenko, Mohammad Reza Pourhaghighi, Hubert Girault EPFL, LEPA, Lausanne, CH 15:00 – 15:30 KN15 Capillary Electrophoresis Mass Spectrometry, the Nerdy Brother of a Giant, Can Be the Answer for Your Next Analytical Challenge David Chen University of British Columbia, Vancouver, CA 15:30 – 16:00 KN16 Capillary Electrophoresis-Mass Spectrometry: Towards Comprehensive Characterization of Intact Biomacromolecules Govert Somsen1, Rob Haselberg2, Elena Dominguez Vega2, Gerhardus de Jong2 1VU University, Amsterdam, NL; 2Utrecht University, Utrecht, NL

Tutorials G104-105

14:00 – 14:40 TU4 Monolithic Silica Columns Nobuo Tanaka GL Science, Tokyo, JP Introduced by Axel Vaast 15:00 – 15:40 TU5 Polymer Monoliths Emily Hilder University of Tasmania, Hobart, AU Introduced by Eva Tyteca

Monday, June 17, 2013 17:00 – 18:00

HYPERformance LC ̶ Fundamentals Auditorium Chair: Fabrice Gritti 17:00 – 17:30 KN5 Properties of the Water-Rich Layer at the Stationary Phase in HILIC – A Molecular Dynamics Study Ulrich Tallarek1, Alexandra Höltzel1, Sergey Melnikov1, Andreas Seidel-Morgenstern2 1Department of Chemistry, Philipps-Universität Marburg, Marburg, DE; 2Max-Planck-Institut für Dynamik komplexer technischer Systeme, Magdeburg, DE 17:30 – 18:00 KN6 Nanoparticles as Probes for Chromatography Edward Yeung1, Gufeng Wang1, Rui Han1, Lehui Xiao1, Dong Xu2, Yan He2 1Iowa State University, Ames, Iowa, US; 2Hunan University, Changsha, Hunan, CN

High-Impact LC ̶ Environmental Analysis Emerald Chair: Gérard Hopfgartner and Martin Giera 17:00 – 17:20 OR18 Separation and Identification of New POPs by Liquid Chromatography and Mass Spectrometry Guibin Jiang, Ting Ruan Chinese Academy of Sciences, Beijing, CN 17:20 – 17:40 OR19 Novel Single and Multi-Dimensional Chromatographic Approaches to a Better Understanding of Dissolved Organic Matter (DOM) Sara Sandron1, Brian Kelleher2, Ekaterina Nesterenko1, Pavel Nesterenko3, Brett Paull3 1DCU-ISSC, Dublin, Ireland; 2DCU-School of Chemistry, Dublin, Ireland; 3ACROSS, Hobart, AU 17:40 – 18:00 DI2 Discussion on needs for environmental analysis

HPLC-MS ̶ Microscale LC-MS and CE-MS G102-103 Chair: Govert Somsen 17:00 – 17:20 OR26 Characterization of Allergens by Various Capillary Electrophoresis Modes Hanno Stutz1, Sergey Gusenkov1, Lorenz Stock1, Chloé Ackaert2, Gertie Janneke Oostingh2 1Division of Chemistry and Bioanalytics/University of Salzburg, Salzburg (Austria), AT; 2Division of Allergy and Immunology/University of Salzburg, Salzburg (Austria), AT 17:20 – 17:40 OR27 Development of Microfluidic Device Combined with Mass Spectrometer for Study of Cell Drug Metabolism Jin-Ming Lin, Qiushui Chen, Dan Gao, Huibin Wei Tsinghua University, Beijing, CN 17:40 – 18:00 OR28 Chip Chromatography in Microfluidic Glass Chips with Optical and Mass Spectrometric Detection Detlev Belder, Claudia Ernst, Sebastian Thürmann Universität Leipzig, Leipzig, DE

Tutorial G104-105

17:00 – 17:40 TU6 Nanofluidics and its Applications to Separations and Sensing Stephen Jacobson Indiana University, Bloomington, IN, US Introduced by Robert-Jan Raterink

Tuesday, June 18, 2013 8:40 – 11:00

HYPERformance LC ̶ Columns Auditorium Chair: Georges Guiochon and Martin Gilar 8:40 – 9:00 Uwe D. Neue Award

9:00 – 9:30 KN17 Optimized Superficially Porous Particles for Protein Analysis Joseph Kirkland, Stephanie Schuster, Brian Wagner, Barry Boyes, Joseph DeStefano Advanced Materials Technology, Inc., Wilmington, DE, US 9:30 – 10:00 KN18 Understanding the Fine Details of the Band Broadening Processes in Liquid Chromatography Gert Desmet, Sander Deridder Vrije Universiteit Brussel, Brussels, BE 10:00 – 10:20 OR29 Active Flow Technology Presenting the ‘Infinite Diameter Column’ in a Curtain Flow Mode of Operation Ross Shalliker1, Harald Ritchie2 1University of Western Sydney, North Parramatta, Australia; 2ThermoFisher, Runcorn, GB 10:20 – 10:40 OR30 The New Cutting Edge in Reversed-Phase UHPLC Performance: 1.3 µm Core- Shell Particles Tivadar Farkas, Mike Chitty, Jeff Layne, Jason Anspach, Carl Sanchez Phenomenex, Inc., Torrance, CA, US 10:40 – 11:00 OR31 A New Particle for Liquid Chromatography Peter Myers1, Haifei Zhang1, Adham Ahmed1, Antony Edge2 1Department of Chemistry, Liverpool, GB; 2ThermoFisher, Runcorn, GB

High-Impact LC ̶ Proteomics Emerald Chair: William Hancock and Alexander Leitner 9:00 – 9:30 KN25 Pre-Fractionation and MS; Condemned to Each Other in Proteomics? Albert J.R. Heck Biomolecular Mass Spectrometry, Utrecht University, Utrecht, NL 9:30 – 10:00 KN26 High-Efficiency and High Peak Capacity Chromatographic Separation Methods for Proteome Analysis Christian G. Huber1, Silke Ruzek1, Katja Melchior2, Lisa Weilnböck1, Nathanale Delmotte2, Hansjörg Toll2, Sascha Quinten2 1Department of Molecular Biology, University of Salzburg, Salzburg, AT; 2Department of Chemistry, Instrumental Analysis and Bioanalysis, Saarland University, Saarbrücken, DE 10:00 – 10:20 OR35 In-House Construction of a UHPLC System Enabling the Identification of over 4,000 Protein Groups in a Single Analysis Alba Cristobal, Marco Hennrich, Piero Giansanti, Soenita S. Goerdayal, Albert J.R. Heck, Shabaz Mohammed Utrecht University, Utrecht, NL 10:20 – 10:40 OR36 Simultaneous Peptide Mapping, Posttranslational Modifications and Major N- Glycosylation Characterization of Trastuzumab by Sheathless Capillary Electrophoresis - Tandem Mass Spectrometry Rabah Gahoual1, Alicia Burr1, Jean-Marc Busnel2, Lauriane Kuhn3, Phillipe Hammann3, Alain Beck4, Yannis-Nicolas Francois1, Emmanuelle Leize-Wagner1 1LSMIS, UMR 7140, University of Strasbourg, Strasbourg, FR; 2Beckman Coulter Inc., Brea, US; 3Proteomics Facility, IBMC, University of Strasbourg, Strasbourg, FR; 4Centre d'Immunologie Pierre Fabre, St Julien-en-Genevois, FR 10:40 – 11:00 OR37 Discovery and Validation of Serum Biomarkers for Cervical Cancer by LC- MS/MS Alexander Boichenko1, Natalia Govorukhina1, Ate van der Zee2, Rainer Bischoff1 1University of Groningen, Groningen, NL; 2University Medical Centre Groningen, Groningen, NL

Tuesday, June 18, 2013 9:00 – 11:10

HPLC-MS ̶ LC-MS of Small Molecules G102-103 Chair: Steve Cohen and Frederic Lynen 9:00 – 9:20 OR45 LC-MSn Study of Biomarkers Metabolites of Human Diseases Bogusław Buszewski1, Małgorzata Szultka1, Stanisław Deja2, Piotr Wieczorek2 1Nicolaus Copernicus University, Toruń, PL; 2Opole University, Opole, PL 9:20 – 9:40 OR46 The Contribution of High-End Analytical Technology to an Integrated Workflow for Valorisation of Bio-Waste Streams Sebastiaan Bijttebier, Els D'Hondt, Stefan Voorspoels VITO NV, Mol, BE 9:40 – 10:00 OR47 Efficiently Combining HPLC, NMR and MS Ulrich Braumann Bruker BioSpin, Rheinstetten, DE 10:00 – 10:30 KN29 Novel Analytical Methods of HPLC-MS Based Metabolomics Xu Guowang, Weidong Dai, Qiang Huang, Shili Chen, Guozhu Ye, Lina Zhou, Peiyuan Yin, Xin Lu Dalian Institute of Chemical Physics, Chinese Academy of Sci, Dalian, CN 10:30 – 11:00 KN30 New Approaches in Nontargeted Lipidomic Quantitation of Biological Samples by LC/MS Michal Holčapek, Eva Cífková, Miroslav Lísa, Blanka Červená University of Pardubice, Pardubice, CZ

Tutorials G104-105

9:30 – 10:10 TU7 MALDI, other Matrix Assisted Ionization Methods, and Nonconventional Approaches to LC/MS Sarah Trimpin Wayne State University, Detroit, MI, US Introduced by: Karli Reiding 10:30 – 11:10 TU8 Quantitative LC-MS Uwe Karst University of Münster, Münster, DE Introduced by: Andjoe Sampat

Tuesday, June 18, 2013 11:30 – 12:30

HYPERformance LC ̶ Columns Auditorium Chair: Frank Svec 11:30 – 12:00 KN19 Designing Polymer Monoliths for the Efficient Chromatography of Small and Large Molecules Emily Hilder, Dario Arrua, Mohammad Talebi, Paul Haddad, Chris Desire University of Tasmania, Hobart, Tasmania, AU 12:00 – 12:30 KN20 On the Morphology, Porous Properties, Polymer Nano-Scale Gel Structure and Related Chromatographic Performance of Porous Polymer Monoliths Ivo Nischang Johannes Kepler University Linz, Leonding, AT

High-Impact LC ̶ Proteomics Emerald Chair: William Hancock and Alexander Leitner 11:30 – 11:50 OR38 Immunohistochemical Staining, Laser Capture Microdissection and FASP Assisted Proteomic Analysis of Cancer Stem Cells within Tissue Samples David Lubman, Jintang He, Jianhui Zhu, Xing Fan University of Michigan, Ann Arbor, MI, US 11:50 – 12:10 OR39 Solid-Phase Based Technologies and Methods for Glycoproteome and Phosphoproteome Analysis Zou Hanfa, Fangjun Wang, Mingliang Ye, Jun Zhu, Zhen Sun, Hongqiang Qin, Junfeng Huang Dalian Institute of Chemical Physics, Dalian, CN 12:10 – 12:30 DI3 Discussion on needs for proteomics

HPLC-MS ̶ LC-MS of Small Molecules G102-103 Chair: Marja-Liisa Riekkola 11:30 – 11:50 OR48 Improvement of 2D (NP/RP) LC-QToF MS Method and its Application in Lipid Profiling of Human Plasma from Peritoneal Dialysis Patients Huwei Liu, Min Li, Yu Bai, Yiping Liao Peking University, Beijing, CN 11:50 – 12:10 OR49 Enantiomeric Characterization of Triacylglycerols and Other Nonpolar Lipids Using Chiral HPLC/MS Miroslav Lisa, Michal Holčapek University of Pardubice, Pardubice, CZ 12:10 – 12:30 OR50 HPLC and MS as Tools for Determination of Stabilizers in Synthetic Polymers: A Way to Improve Quality and to Understand the Performance of Plastic Materials Wolfgang Buchberger, Susanne Beissmann, Ingrid Hintersteiner, Michael Reisinger, Lucas Sternbauer, Martin Stiftinger Institute of Analytical Chemistry, University Linz, Linz, AT

Tutorial G104-105

11:50 – 12:30 TU9 Comprehensive Two-Dimensional Liquid Chromatography Paola Dugo University of Messina, Messina, IT Introduced by: Anna Baglai

Tuesday, June 18, 2013 14:00 – 16:00

HYPERformance LC ̶ Instruments/Data Analysis Auditorium Chair: Pat Sandra and Dwight Stoll 14:00 – 14:30 KN21 Theoretical and Practical Performance Limits of High Efficiency Small-Bore UHPLC Columns in Isocratic and Gradient Separations Monika Dittmann1, Bernd Hoffmann1, William Barber2, Xiaoli Wang2, Ta-Chen Wei2 1Agilent Technologies, Waldbronn, DE; 2Agilent Technologies, Wilmington, US 14:30 – 15:00 KN22 Nanopore Devices for Virus Particle Characterization Stephen Jacobson, Zachary Harms, Laurence Kohler, Lisa Selzer, Lye Siang Lee, Adam Zlotnick Indiana University, Bloomington, IN, US 15:00 – 15:20 OR32 High-Pressure Miniaturized Electro-Osmotic Pump for Capillary HPLC Shaorong Liu, Congying Gu, Zaifang Zhu, Wei Wang, Aaron Morgan, Joann Lu1 University of Oklahoma, Norman, US 15:20 – 15:40 OR33 Half-Width Plots: A Simple Tool to Reveal Column Kinetics and Characterize Chromatographic Columns María Celia García-Álvarez-Coque, Juan José Baeza-Baeza, María José Ruiz-Ángel, José Ramón Torres-Lapasió, Samuel Carda-Broch University of Valencia, Burjassot (Valencia), ES 15:40 – 16:00 OR34 Characterizing Extra-Column Effects in Ultra-High Pressure Liquid Chromatography James Grinias1, Martin Gilar2, Jeremy Rose2, James Jorgenson1 1UNC Chapel Hill, Chapel Hill, NC, US; 2Waters Corp., Milford, MA, US

High-Impact LC ̶ Polymer Separations Emerald Chair: Gert Desmet and Ron Peters 14:00 – 14:30 KN27 Challenging the Present State of the Art of Polymer Separations Mark Schure1, Wei Gao2 1Superon GmbH and Kroungold Analytical, Inc., Blue Bell, PA 19422, US; 2The Dow Chemical Company, Springhouse, PA 19477, US 14:30 – 15:00 KN28 SEC-Gradients: A New Approach to the Separation of Polymers by Chemical Composition Wolfgang Radke Fraunhofer Institute for Structural Durability and System Reliability, Darmstadt, DE 15:00 – 15:20 OR40 Efficiency in (Ultra-High-Pressure) Size-Exclusion Chromatography Elena Uliyanchenko1, Sjoerd van der Wal2, Peter Schoenmakers3 1SABIC, Bergen op Zoom, NL; 2DSM Resolve, Geleen, NL; 3University of Amsterdam, Amsterdam, NL 15:20 – 15:40 OR41 Advances in Size Characterization of Synthetic Polymers Edouard Bouvier, Bonnie Alden, Mia Summers, Jeremy Shia, Jessica Wilson, Michael Savaria, Kevin Wyndham, Thomas Walter Waters Corporation, Milford, US 15:40 – 16:00 OR42 Enabling Polymer Characterization via Unique Separation Selectivities and Mechanisms Rob Edam1, Rongjuan Cong2, Willem deGroot2, Dean Lee3, John Lyons3, Edwin Mes1, David Meunier3, Randy Pell3, Ted Stokich Jr.3, Peter Schoenmakers4, Bill Winniford2, Matthew Miller2 1Dow Benelux B.V., Terneuzen ZE, NL; 2Dow Chemical, Freeport, US; 3Dow Chemical, Midland, US; 4University of Amsterdam, Amsterdam, NL

Tuesday, June 18, 2013 14:00 – 16:00

HPLC-MS ̶ Biomedical LC-MS G102-103 Chair: Xu Guowang and Petra Aarnoutse 14:00 – 14:20 OR51 Recent Progress in Core-shell and Embedded Nanoparticle Layers for the Separation and Sensing of Biomarkers and Cell Signalling Molecules Lin Zhou1,2, Gerard P. McGlacken2, John H.T. Luong3, Jeremy D. Glennon1,2* 1Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), 2Department of Chemistry and the Analytical &Biological Chemistry Research Facility (ABCRF), University College Cork, IE; 3Biotechnology Research Institute, National Research Council Canada, Montreal, Quebec, CA 14:20 – 14:40 OR52 Vitamin-D Metabolite Metrology Lane Sander, Karen Phinney, Mary Bedner, Johanna Camara, Katrice Lippa, Susan Tai, Stephen Wise National Institute of Standards and Technology, Gaithersburg, MD, US 14:40 – 15:00 OR53 High-Speed Gradient Separations of Peptides and Proteins using Polymer- Monolithic Poly(styrene-co-divinylbenzene) Capillary Columns at Ultra-High Pressure Axel Vaast1, Lucie Nováková1, Bjorn de Haan2, Remco Swart2, Sebastiaan Eeltink1 1Vrije Universiteit Brussel, Brussels, BE; 2Thermo Fisher Scientific, Amsterdam, NL 15:00 – 15:30 KN31 The Role of Mass Spectrometry in Post-Column On-Line/At-Line Bioactivity Screening Wilfried Niessen VU University Amsterdam, Amsterdam, NL 15:30 – 16:00 KN32 Strategy for Chromatographic Analysis of Glycans in Medicinal Plants Shaoping Li University of Macau, MO

Tutorials G104-105

14:00 – 14:40 TU10 Chiral Separations Michael Lämmerhofer University of Tübingen, Tübingen, DE Introduced by: Debby Mangelings 15:00 – 15:40 TU11 Column Efficiency Fabrice Gritti The University of Tennessee, Knoxville, TN, US Introduced by: Michelle Camenzuli

Tuesday, June 18, 2013 17:00 – 18:00

HYPERformance LC ̶ Data Analysis Auditorium Chair: Jan Christensen 17:00 – 17:30 KN23 On the Use of Bayesian Statistics for the Analysis of Chromatographic Data: A New Generation of Data-Treatment Methods? Gabriel Vivo-Truyols University of Amsterdam, Amsterdam, NL 17:30 – 18:00 KN24 Herbal Fingerprints: Extraction of Information, Focussing on Similarity Analyses Yvan Vander Heyden, Goedele Alaerts, Melanie Dumarey, Mohammad Goodarzi, Christophe Tistaert, Bieke Dejaegher: Vrije Universiteit Brussel (VUB), Brussels, BE

High-Impact LC ̶ Polymer Separations Emerald Chair: Gert Desmet and Ron Peters 17:00 – 17:20 OR43 Separation and Characterization of Targeting Biomedical Polymers by HPLC Xulin Jiang1, Yanfeng Chu1, Jia Liu1, Renxi Zhuo1, Peter J. Schoenmakers2 1Wuhan University, Wuhan, CN; 2University of Amsterdam, Amsterdam, NL 17:20 – 17:40 OR44 Understanding Retention of Block Co-Polymers in SEC and LCCC J. Ilja Siepmann1, Kim Struk1, Tim Lodge1, Mark Schure2 1University of Minnesota, Minneapolis, US; 2Kroungold Analytical Inc, Blue Bell, US 17:40 – 18:00 DI4 Discussion on needs for polymer analysis

HPLC-MS ̶ Biopharmaceutical LC-MS G102-103 Chair: Robert Kennedy 17:00 – 17:20 OR54 LCxLC Hyphenated to Q-TOF MS for the Characterization of Protein Biopharmaceuticals Gerd Vanhoenacker1, Koen Sandra2, Isabel Vandenheede2, Frank David1, Pat Sandra1 1Research Institute for Chromatography, Kortrijk, BE; 2Metablys, Kortrijk, BE 17:20 – 17:40 OR55 Synthesis of a Neo-Glyco-Vaccine against Tuberculosis: The Key Role of Liquid Chromatography-Mass Spectrometry Caterina Temporini, Teodora Bavaro, Immacolata Serra, Sara Tengattini, Enrica Calleri, Marco Terreni, Gabriella Massolini Dept. Drug Sciences University of Pavia, Pavia, IT 17:40 – 18:00 OR56 Rapid qualitative and Quantitative Characterization of Antibody Glycosylation Profiles by Native Mass Spectrometry Using an Orbitrap Mass Analyzer Sara Rosati1, Ewald T.J. van den Bremer2, Paul Parren2, Janine Schuurman2, Albert J. R. Heck1 1Utrecht University, Utrecht, NL; 2Genmab, Utrecht, NL

Tutorial G104-105

17:00 – 17:40 TU12 Towards Quantitative Morphology-transport Relationships for Chromatographic Stationary Phases Ulrich Tallarek Philipps-Universität Marburg, Marburg, DE Introduced by: Henrik Cornelisson van de Ven

Wednesday, June 19, 2013 8:40 – 11:00

HYPERformance LC ̶ Selectivity Auditorium Chair: Wolfgang Buchberger and Stefanie Wernisch 8:40 – 9:00 High-Performance Lindner Chromatography (sessions sponsored by Millipore) Presented by Michael Lämmerhofer and Barry Karger

9:00 – 9:30 KN33 The Integration of Liquid Chromatography and Mass Spectrometry for Ultratrace Analysis Barry Karger Barnett Institute/Northeastern University, Boston, US 9:30 – 10:00 KN34 Studying Protein Complexes and Biological Networks by Chemical Cross- Linking and Mass Spectrometry Alexander Leitner, Thomas Walzthoeni, Franz Herzog, Abdullah Kahraman, Ruedi Aebersold ETH Zurich, Institute of Molecular Systems Biology, Zurich, CH 10:00 – 10:20 OR57 Ion-Exchange, Hydrophilic and Hydrophobic Interactions Affecting Selectivity for Neutral and Charged Solutes on Agglomerated Ion-Exchange and Commercial Mixed-Mode Stationary Phases Brett Paull1, Artaches Kazarian1, Paul Haddad1, Pavel Nesterenko1, Mark Taylor2 1Australian Centre for Research on Separation Science, Hobart, Australia; 2Pfizer Global Research and Development, Sandwich, GB 10:20 – 10:40 OR58 –Polymer-Coated Or Not? – A Simple Test Method to Categorize Hydrophilic Interaction Chromatography (HILIC) Phases Based on their Structural Selectivity Tohru Ikegami1, Nobuo Tanaka2 1Kyoto Institute of Technology, Kyoto, JP; 2GL Science, Iruma, JP 10:40 – 11:00 OR59 The Impact of Imidazolium Substituents on the Retention Properties of Surface-Confined Ionic Liquid Stationary Phases for HPLC – Revisited Apryll M. Stalcup: Irish Separation Science Cluster/Dublin City University, Dublin 9, IE

High-Impact LC ̶ Food Emerald Chair: Michel Nielen and Erwin Kaal 9:00 – 9:30 KN41 Food Analysis: A Challenging Area for Liquid Chromatography and Mass Spectrometry Hans-Gerd Janssen Unilever Research Vlaardingen, Vlaardingen, NL 9:30 – 10:00 KN42 Advanced Liquid Chromatography and Mass Spectrometry Techniques for Lipid Analysis Paola Dugo1, Paola Donato2, Francesco Cacciola1, Luigi Mondello1 1University of Messina, Messina, IT; 2University Campus Biomedico, Rome, IT 10:00 – 10:20 OR63 Orbitrab vs QTOF to Determine Pesticide Residues in Fruit and Vegetable Extracts Yolanda Picó1, Marinella Farré2, Damià Barceló2 1Faculty of Pharmacy, University of Valencia, Burjassot, ES; 2IDAEA/CSIC, Barcelona, ES 10:20 – 10:40 OR64 The Serial RP-HPLC/HILIC/API-MS Coupling: An Easy, Robust and Versatile Technique Giorgia Greco, Thomas Letzel Technische Universität München, Garching, DE 10:40 – 11:00 OR65 Discovery of Multiple Sites of Intra-Molecular Protonation and Different Fragmentation Patterns within the Fluoroquinolone Class of Antibiotics in Porcine Muscle Extracts Using Ion Mobility Mass Spectrometry Sara Stead1, Mike McCullagh1, Antonietta Gledhill1, Wouter deKeizer2, Aldert Bergwerff2 1Waters corp, Manchester, GB; 2RnAssays, Utrecht, NL 29

Wednesday, June 19, 2013 9:00 – 11:10

HPLC-MS ̶ High-resolution MS G102-103 Chair: Manfred Wuhrer and Imma Ferrer 9:00 – 9:20 OR73 Glycosylation Analysis of Enriched Immunoglobulin A Dennis Blank1, Albert Bondt2, Simone Nicolardi1, Yuri E.M. Burgt1, André M. Deelder1, Manfred Wuhrer1 1Leiden University Medical Center - LUMC, Leiden, NL; 2Erasmus University Medical Center, Rotterdam, NL 9:20 – 9:40 OR74 Use of Liquid Chromatography- High Resolution Mass spectrometry (LC-HRMS) in Bioanalytical Assays in Combination with Micro-sampling of Biological Fluids Monica P. Cala Molina, Juan P. Rincon Pabon, Roland J.W. Meesters Universidad de los Andes, Bogota D.C., CO 9:40 – 10:00 OR75 At the Edge of One-Dimensional HT-UHPLC-MS in an Industrial Environment Ron Peters, Ynze Mengerink, Sjoerd van der Wal DSM Resolve, Geleen, NL 10:00 – 10:30 KN45 The Role of Molecular Flash Photography in Desorption, Detection and Disease Ron M.A. Heeren FOM-AMOLF, Amsterdam, NL 10:30 – 11:00 KN46 Orbitrap Mass Spectrometry: Past, Present and Future Alexander Makarov ThermoFisher Scientific (Bremen), Bremen, DE

Tutorials G104-105

9:30 – 10:10 TU13 System Performance Gert Desmet Free University Brussels, Brussels, BE Introduced by: Rudy Vonk 10:30 – 11:10 TU14 Role of the Stationary Phase in HPLC Attila Felinger University of Pécs, Pécs, HU Introduced by: Daniela Peroni

Wednesday, June 19, 2013 11:30 – 12:30

HYPERformance LC ̶ Selectivity Auditorium Chair: Paul Haddad 11:30 – 12:00 KN35 Influence of Phase Type and Solute Structure on Changes in Retention with Pressure in Reversed-Phase High Performance Liquid Chromatography David McCalley1, Stephan Buckenmaier2, Nobuo Tanaka3, Morgane Fallas1 1University of the West of England, Bristol, Bristol, GB; 2Agilent Technologies, Waldbronn, DE; 3GL Sciences, Kyoto, JP 12:00 – 12:30 KN36 Effect of Pressure on Retention and Selectivity of Polymeric ODS and C30 Stationary Phase in Reversed-Phase HPLC. Increased Separation of Isomeric Fatty Acids, Fatty Acid Methyl Esters, Triacylglycerols, and Tocopherols at High Pressure Nobuo Tanaka1, Kensuke Okusa2, Ikuma Kuroda2, Masayoshi Ohira2, Toshiharu Nagai3, Hoyo Mizobe3, Naohiro Gotoh4, Tohru Ikegami5, David V. McCalley6 1GL Sciences, Inc., Kyoto, JP; 2GL Sciences, Inc., Iruma, JP; 3Tsukishima Foods Industry, Toyko, JP; 4Tokyo University of Marine Science and Technology, Tokyo, JP; 5Kyoto Institute of Technology, Kyoto, JP; 6University of the West of England, Bristol, GB

High-Impact LC ̶ Food Emerald Chair: Michel Nielen and Erwin Kaal 11:30 – 11:50 OR66 Recent Developments in the Liquid Chromatographic Analysis of Anthocyanins André de Villiers, Chandré Willemse, Philippus Alberts, Maria Stander Stellenbosch University, Stellenbosch, SA 11:50 – 12:10 OR67 The Analysis of Natural Products Using Active Flow Technology with Multiplexed Detection Michelle Camenzuli1, Harald Ritchie2, R. Andrew Shalliker1 1University of Western Sydney, Parramatta, NSW, Australia; 2Thermo Fisher Scientific, Runcorn, WA7 1TA, GB 12:10 – 12:30 DI5 Discussion on needs for food analysis

HPLC-MS ̶ LC-MS Data Analysis G102-103 Chair: Yvan Vander Heyden 11:30 – 12:00 KN47 Signal Processing - The Missing Link in Analysis of LC, LC-MS and LC×LC Data Jan Christensen1, Nikoline Nielsen1, Rune Græsbøl1, Søren Furbo1, Giorgio Tomasi2 1Plant and Environmental Sciences, University of Copenhagen, Frederiksberg C, Denmark; 2European Commission Joint Research Centre, Institute for Health and Consumer Protection, ISPRA, IT 12:00 – 12:30 KN48 Recent Developments and Applications of Metalign-Based Data Processing in Chromatography Combined with Full Scan Mass Spectrometry Arjen Lommen, Paul Zomer, Michel Nielen, Hans Mol RIKILT - Institute of Food Safety, Wageningen UR, Wageningen, NL

Tutorial G104-105

11:50 – 12:30 TU15 Supercritical Fluid Chromatography Isabelle Francois Waters, Brussels, BE Introduced by: Hanneke Brust

Wednesday, June 19, 2013 14:00 – 16:00

HYPERformance LC ̶ Selectivity Auditorium Chair: Christian Huber and Helmut Hinterwirth 14:00 – 14:20 OR60 HPLC Performance of Zwitterionic Chiral Stationary Phases in Direct Enantiomer Separation of Amino Acids and Other Ionisable Compounds Pilar Franco1, Tong Zhang1, Emilie Holder1, Wolfgang Lindner2 1Chiral Technologies Europe, Illkirch, FR; 2University of Vienna, Department of Analytical Chemistry, Vienna, AT 14:20 – 14:40 OR61 Chiral Amino Acid Metabolome Analysis for Biomarker Assay in Clinical Diagnosis Using Enantioselective 2D-HPLC Kenji Hamase1, Yurika Miyoshi1, Nao Kusano1, Jumpei Sasabe2, Yosuke Tojo3, Masashi Mita3, Sadakazu Aiso2, Wolfgang Lindner4 1Kyushu University, Fukuoka, JP; 2Keio University, Tokyo, JP; 3Shiseido Co., Ltd., Tokyo, JP; 4University of Vienna, Vienna, AT 14:40 – 15:00 OR62 Optimizing Selectivity for HPLC Separations of Charged and Neutral Polar Compounds Egidijus Machtejevas: Merck Millipore / MM-L Lab Essentials, Darmstadt, DE 15:00 – 15:30 KN37 Purification of Baculovirus and Influenza VLP by Monoliths Alois Jungbauer, Petra Gerster, Reingard Grabherr: BOKU, Vienna, AT 15:30 – 16:00 KN38 Nanoparticle-Based Selective Sample Preparation for HPLC-MS/MS Analysis of Oxidized Phospholipids as Biomarkers for Oxidative Stress Michael Lämmerhofer1, Helmut Hinterwirth2, Elisabeth Haller3, Wolfgang Lindner3 1Institute of Pharmaceutical Sciences, Univ. of Tuebingen, Tuebingen, DE; 2Institute ofAnal. Chem., Univ. of Vienna, Vienna, AT; 3Institute of Anal. Chem., Univ. of Vienna, Vienna, AT

High-Impact LC ̶ Metabolomics Emerald Chair: DavidMcCalley and Sebastiaan Eeltink 14:00 – 14:30 KN43 Towards an Optimal LC-MS/MS Analysis Platform for Identification and Quantitation of Small molecules in Body Fluid Karl-Siegfried Boos, Qianqian Yu, Sena Caglar, Rosa Morello Laboratory of BioSeparation, Medical Center LMU, Munich, DE 14:30 – 15:00 KN44 Analytical Solutions to Address the Challenges of Mass-Spectrometry Based Metabolomics Thomas Hankemeier: Netherlands Metabolomics Centre/Leiden University, Leiden, NL 15:00 – 15:20 OR68 State-Of-The-Art LC-MS Based Lipidomics Applied in The Study of Lung Diseases Koen Sandra1, Ruben t'Kindt1, Eef Telenga2, Lucie Jorge1, Antoon van Oosterhout2, Nick ten Hacken2, Pat Sandra1: 1RIC/Metablys, Kortrijk, BE; 2UMCG, Groningen, NL 15:20 – 15:40 OR69 Evaluation of Different Column Chemistries for Fast Urinary Metabolic Profiling Martin Giera1, Dick-Paul Kloos1, Henk Lingeman2, André Deelder1, Wilfried Niessen2, Oleg Mayboroda1 1Leiden University Medical Center (LUMC), Leiden, NL; 2VU University, Amsterdam, NL 15:40 – 16:00 OR70 Molecular Cartography in Acute Chlamydia Pneumoniae Infections – A Non- Targeted Metabolomics Approach Constanze Müller1, Inga Dietz2, Dimitrios Tziotis3, Franco Moritz4, Jan Rupp5, Philippe Schmitt- Kopplin4: 1Research unit Analytical BioGeoChemistry, Helmholtz Zentrum, Neuherberg, DE; 2University of Lübeck, Lübeck, DE; 3Institute for Medical Research unit Analytical BioGeoChemistry, Helmholtz Zentrum München, Neuherberg, DE; 4Research unit Analytical BioGeoChemistry, Helmholtz Zentrum München, Neuherberg, DE; 5Institute for Medical Microbiology and Hygiene, University of Lübeck, Lübeck, DE 32

Wednesday, June 19, 2013 14:00 – 16:00

HPLC-MS ̶ LC-MS Data Analysis G102-103 Chair: Koji Otsuka and Emmie Ho 14:00 – 14:20 OR76 How to Assess Uncertainties in the Mass Spectrometric Confirmation Bjorn Berendsen1, Linda Stolker1, Michel Nielen2 1RIKILT, Wageningen UR, Wageningen, NL; 2Wageningen University, Wageningen, NL 14:20 – 14:40 OR77 Parallel LC-MALDI and MALDI Imaging Workflow for Direct Identification of Biomarkers from Tissue Michael Becker1, Martin Schuerenberg1, Rainer Paape1, Detlev Suckau1, Axel Walch2, Sandra Rauser2 1Bruker Daltonik GmbH, Bremen, DE; 2Helmholtz Zentrum München, Institut für Pathologie, Munich, DE 14:40 – 15:00 OR78 Full plasma N-Glycoproteomics Using GlycoFrag and HILIC Micro-SPE Enrichment Bas Jansen, Leonardo Lenoci, Maurice Selman, Dennis Blank, Gerhild Zauner, Magnus Palmblad, André Deelder, Manfred Wuhrer Leiden University Medical Center, Leiden, NL 15:00 – 15:30 KN49 New Approaches to High-Throughput Protein and Protein-Protein Interaction Analysis Using Capillary and Microchip Electrophoresis Robert Kennedy University of Michigan, Ann Arbor, MI, US 15:30 – 16:00 KN50 Bigger is Better: Challenges in Pharmaceutical Microtechnology John Frenz GlobeImmune, Inc., Louisville, CO, US

Tutorials G104-105

14:00 – 14:40 TU16 Environmental Analysis Marja-Liisa Riekkola University of Helsinki, Helsinki, FI Introduced by: Alexander Boichenko 15:00 – 15:40 TU17 Lipidomic Analysis Michal Holčapek University of Pardubice, Pardubice, CZ Introduced by: Jana Králová

Wednesday, June 19, 2013 17:00 – 18:00

HYPERformance LC ̶ Selectivity Auditorium Chair: Nobuo Tanaka 17:00 – 17:30 KN39 The impact of Quality by Design on Chromatography Method Development Fritz Erni Dornach, CH 17:30 – 18:00 KN40 Selectivity Tuning of Chiral Ion Exchanger Type Stationary Phases via Chemical Structure and Mobile Phase Variations in HPLC and SFC Mode Wolfgang Lindner1, Denise Wolrab1, Michal Kohout2 1University of Vienna, Vienna, AT; 2Dept. of Organic Chemistry, Prague, CZ

High-Impact LC ̶ Metabolomics Emerald Chair: DavidMcCalley and Sebastiaan Eeltink 17:00 – 17:20 OR71 State-Of-The-Art Hyphenated Techniques HPLC/DAD-MS/SPE/NMR Interface for New Approches by High Throughput Sensitivity Medicinal Chemistry, Pharmacognosy, Metabolomic and Translational Projects Cyril Antheaume, Nicole Rimlinger, Veronika Cakova Université de Strasbourg, Illkirch, FR 17:20 – 17:40 OR72 3-Phase Electroextraction: A New (Online) Sample Purification and Enrichment Method for Bioanalysis Robert-Jan Raterink, Peter Lindenburg, Rob Vreeken, Thomas Hankemeier Leiden University, LACDR, NMC, Leiden, NL 17:40 – 18:00 DI6 Discussion on needs for metabolomics

HPLC-MS ̶ LC-MS of Large Molecules G102-103 Chair: Uwe Karst 17:00 – 17:20 OR79 Sample Displacement Chromatography for Protein Purification Hartmut Schlüter, Marta Kotasinska, Verena Richter, Robert Ahrends, Diana Hildebrand, Maria Trusch University Medical Center Hamburg-Eppendorf, Hamburg, DE 17:20 – 17:40 OR80 Coupling of Protein Prefractionation with LC-MS/MS Using Electrospun Nanofibers Wenwan Zhong, Hui Wang, Samantha Schachermeyer University of California, Riverside, Riverside, US 17:40 – 18:00 OR81 Electrochemically Initiated Reactions Upfront MS - EC/MS an Unknown Panacea? Jean-Pierre Chervet, Agnieszka Kraj, Hendrik-Jan Brouwer, Martin Eysberg Antec, Zoeterwoude, NL

Tutorial G104-105

17:00 – 17:40 TU18 Residue Analysis Yolanda Picó University of Valencia, Valencia, ES Introduced by: Petra Dinisova

Thursday, June 20, 2013 9:00 – 11:00

HYPERformance LC ̶ LCxLC Auditorium Chair: Paola Dugo and Rob Edam 9:00 – 9:30 KN51 Comparison of Selective and Fully Comprehensive Two-Dimensional HPLC Dwight Stoll1, David Harmes1, Joe Davis2 1Gustavus Adolphus College, St. Peter, MN, US; 2Southern Illinois University, Carbondale, Carbondale, IL, US 9:30 – 10:00 KN52 The Quest for Ultra-High Peak Capacities - Spatial Three-Dimensional Chromatography Bert Wouters, Jelle De Vos, Axel Vaast, Sebastiaan Eeltink Vrije Universiteit Brussel, Brussels, BE 10:00 – 10:20 OR82 Comprehensive Two-dimensional LCxLC Using Combinations of Monolithic Capillary Columns and Short Core-Shell Columns Pavel Jandera, Magda Stankova, Tomas Hajek, Petr Cesla, Katerina Vynuchalova University of Pardubice, Pardubice, CZ 10:20 – 10:40 OR83 On-line LCxLC Hyphenation by a Machine Vision Controlled Solvent Evaporation Interface Jan-Willem Schoonen, Paul Vulto, Heiko van der Linden, Thomas Hankemeier Netherlands Metabolomics Centre, Leiden, NL 10:40 – 11:00 OR84 Rigorous Optimization of LC×LC with Gradient Elution in Both Dimensions Sandra Pous Torres1, Gabriel Vivó Truyols2, José Ramón Torres Lapasió1, María Celia García Álvarez-Coque1, Peter Schoenmakers2 1Analytical Chemistry Department. University of Valencia, Burjassot, ES; 2Analytical Chemistry Group, Van 't Hoff Institute for Molecular Sciences, University of Amsterdam, Amsterdam, NL

High-Impact LC ̶ Forensics Emerald Chair: Emily Hilder and Bjorn Berendsen 9:00 – 9:30 KN55 Forensic Challenges for LC and LC-MS Arian van Asten, John Gonsalvez, Bas Teunissen, Xiaoma Xu Netherlands Forensic Institute, The Hague, NL 9:30 – 10:00 KN56 Modern Forensic Applications of LC and LC-MS Emmie Ho, W.H. Kwok, David Leung, Gary Leung, April Wong, Colton Wong, Jenny Wong, Nola Yu, Terence Wan The Hong Kong Jockey Club, Sha Tin, N.T., HK 10:00 – 10:20 OR88 Determination of 2-Methylthiazolidine-4-carboxylic acid (MTCA) a Potential Marker for Ethanol Consumption by HPLC-ESI-MS/MS Roland Reischl1, Wolfgang Bicker2, Thomas Keller1, Wolfgang Lindner3 1Paris Lodron University Salzburg, Salzburg, AT; 2FTC Forensic Toxicology Lab Ltd, Vienna, AT; 3University of Vienna, Vienna, AT 10:20 – 10:40 OR89 Gas-Phase Separation of Drugs of Abuse and their Metabolites from Tissue Sections by Differential Ion Mobility Spectrometry Tiffany Porta, Emmanuel Varesio, Gérard Hopfgartner University of Geneva, Geneva, CH 10:40 – 11:00 OR90 Information Dependent Acquisition with LC-MS/MS as a Tool for Screening of 453 Compounds in Forensic Urine and Whole Blood Samples Ruth Verplaetse, Sylvie Decabooter, Eva Cuypers, Jan Tytgat KU Leuven, Laboratory Toxicology, Leuven, BE

Thursday, June 20, 2013 9:00 – 11:10

HPLC-MS ̶ LC-MS of Large Molecules G102-103 Chair: Barry Karger and Hanfa Zou 9:00 – 9:20 OR93 A Linear pH Gradient Separation Platform for Monoclonal Antibody (MAb) Charge Variant Analysis Shanhua Lin1, Wim Decrop2, Julia Baek1, Srinivasa Rao1, Yury Agroskin1, Chris Pohl1 1Thermo Fisher Scientific, Sunnyvale, US; 2Thermo Fisher Scientific, Amsterdam, NL 9:20 – 9:40 OR94 Studying the MMP-9 – TIMP-1 Protease-Inhibitor System by Activity-Based Enrichment and Selected Reaction Monitoring (SRM) Nano-LC-MS/MS Laurette Prely1, Anouk Oldenburger1, Jos Hermans1, Krisztina Paal1, Riccardo Castelli2, Nico C. van de Merbel1, Antoon J.M. van Oosterhout1, Herman S. Overkleeft3, Martina Schmidt1, Rainer Bischoff1 1University of Groningen, Groningen, NL; 2Leiden University, Groningen, NL; 3Leiden University, Leiden, NL 9:40 – 10:00 OR95 Peptide Behaviour in RP-HPLC Coupled to MS: Practical Considerations Based on a Quantitative Structure-Retention Relationship Study Virginie Houbart, Anne-Catherine Servais, Jacques Crommen, Marianne Fillet University of Liège, Liège, BE 10:00 – 10:30 KN59 Protein Glycosylation Analysis by LC-MS Manfred Wuhrer, Maurice H.J. Selman, Cees Bruggink, Crina I.A. Balog, Zauner, Carolien A.M. Koeleman, Paul J. Hensbergen, André M. Deelder Leiden Univ. Medical Center, Leiden, NL 10:30 – 11:00 KN60 Genome-Wide Proteomics: Gastric Cancer Transcriptomics/Proteomic Signatures William Hancock Northeastern University, Boston, MA, US

Tutorials G104-105

9:30 – 10:10 TU19 The Nanoliter Lab: Using Droplets in Analytical and Separation Science Robert Kennedy University of Michigan, Ann Arbor, MI, US Introduced by: Marija Mladic 10:30 – 11:10 TU20 How to Understand and Accomplish Separations Using Hydrophilic Interaction Chromatography (HILIC) David McCalley University of Western England, Bristol, UK Introduced by: Blanka Červená

Thursday, June 20, 2013 11:30 – 12:30

HYPERformance LC ̶ SFC Auditorium Chair: Hans-Gerd Janssen 11:30 – 12:00 KN53 Determination of Equilibrium Isotherms in Supercritical Fluid Chromatography Georges Guiochon, Fabrice Gritti, Abhijit Tarafder, Fahimeh Kamarei University of Tennessee, Knoxville, TN, US 12:00 – 12:30 KN54 Towards a Better Understanding of Enantioselective SFC Caroline West, Syame Khater ICOA, CNRS UMR 7311, University of Orléans, Orléans, FR

High-Impact LC ̶ Forensics Emerald Chair: Emily Hilder and Bjorn Berendsen 11:30 – 11:50 OR91 Rapid Separations of Improvised Explosives Michael Breadmore, Cameron Johns, Adam Gaudry, Parvez Mahbub, Phil Zakaria, Joseph Hutchinson, Emily Hilder, Greg Dicinoski, Rosanne Guijt, Pavel Nesterenko, Mirek Macka, Paul Haddad University of Tasmania, Hobart, Tasmania, AU 11:50 – 12:10 OR92 Accurate Quantitation of Explosive Residues by Liquid Chromatography-Mass Spectrometry (LC-MS) to Investigate Possible Crime-Scene Presence Hanneke Brust1, Arian van Asten2, Mattijs Koeberg2, Chris-Jan Kuijpers2, Antoine van der Heijden3, Peter Schoenmakers1 1University of Amsterdam, Amsterdam, NL; 2Netherlands Forensic Institute, The Hague, NL; 3TNO Technical Sciences, Rijswijk, NL 12:10 – 12:30 DI7 Discussion on needs for forensics

HPLC-MS ̶ LCxLC-MS G102-103 Chair: Michal Holcapek 11:30 – 11:50 OR96 Analysis of Chinese Herbal Medicine with LC×LC-MS Oliver Schmitz1, Roman Dück2, Duxin Li1 1University of Duisburg-Essen, Essen, DE; 2University of Wuppertal, Wuppertal, DE 11:50 – 12:10 OR97 Two-dimensional HPLC with Hyphenated UV-MS-CAD Detection in Pharmaceutical Analysis Kelly Zhang; Genentech Inc., South San Francisco, CA, US 12:10 – 12:30 OR98 Combining Different Analytical Methods for Glycan Characterization of Cancer Tissues Stephanie Holst1, Crina I. A. Balog1, Kathrin Stavenhagen1, Carolien A. M. Koeleman1, Liam M. McDonnell1, Oleg A. Mayboroda1, Aswin Verhoeven1, Wilma E. Mesker2, Rob A. E. M. Tollenaar2, André M. Deelder1, Manfred Wuhrer1 1LUMC, Center for Proteomics and Metabolomics, Leiden, NL; 2LUMC, Department of Surgery, Leiden, NL

Tutorial G104-105

11:50 – 12:30 TU21 LC x LC of Polymers Wolfgang Radke Fraunhofer Institute for Structural Durability and System Reliability LBF, Darmstadt, DE Introduced by: Maria Marioli

Thursday, June 20, 2013 13:30 – 14:30

HYPERformance LC ̶ SFC Auditorium Chair: Isabelle Francois 13:30 – 13:50 OR85 Possibilities and Limitations of SFC and SFC-MS/MS Using State-Of-The-Art Instrument and Innovative Stationary Phases Davy Guillarme, Alexandre Grand-Guillaume Perrenoud, Szabolcs Fekete, Jean-Luc Veuthey University of Geneva, Geneva, CH 13:50 – 14:10 OR86 Packed Column SFC: 1 or 2 Powerful Dimension(s) in 2D Separation Techniques Didier Thiebaut, Pierre Guibal, Orjen Petkovic, Patrick Sassiat, Jerome Vial ESPCI Paristech - LSABM, Paris Cedex 05, FR 14:10 – 14:30 OR87 Production Rate Optimization in (Semi-)Preparative Chromatography via Chiral and Achiral Stationary Phase Optimized LC and SFC Frederic Lynen, Sander Delahaye, Kai Chen Ghent University, Ghent, BE

High-Impact LC ̶ Sample Preparation Emerald Chair: John Frenz 13:30 – 14:00 KN57 Nanoparticles and Thin Films for Sample Preparation and Detection Frantisek Foret, Pavel Podesva, Petra Juskova, Jana Krenkova Inst.Anal.Chem. ASCR, v.v.i., Brno, CZ 14:00 – 14:30 KN58 Towards High-Sensitive Detection in Microscale Electrophoresis Using On-Line Sample Concentration Koji Otsuka1, Hiroshi Koino1, Saeko Kinami1, Hiroya Ota1, Takayuki Kawai2, Kenji Sueyoshi1, Takuya Kubo1, Fumihiko Kitagawa3 1Kyoto University, Kyoto, JP; 2National Institute of Advanced Industrial Science and Technology, Osaka, JP; 3Hirosaki University, Aomori, JP

HPLC-MS ̶ Microscale LC-MS G102-103 Chair: Thomas Hankemeier 13:30 – 14:00 KN61 Advances and Applications of UPLC-MS to Endogenous and Drug Metabolite Profiling Ian Wilson Imperial College, London, GB 14:00 – 14:30 KN62 Making an Integrated, Versatile Microfluidic UHPLC System Reality Steven Cohen, Paul Rainville, Jay Johnson, Angela Doneanu, Jim Murphy Waters Corporation, Milford MA, US

Thursday, June 20, 2013 14:50 – 17:00

Plenary Session Auditorium Chair: Wolfgang Lindner and Attila Felinger 14:50 – 15:20 PL5 Interfacing Liquid Chromatography with Mass Spectrometry: From Zero Plate Chromatography to Multidimensional Approaches Gérard Hopfgartner, Emmanuel Varesio University of Geneva, Geneva, CH 15:20 – 15:50 PL6 Microfluidic Separation Devices Coupled to Mass Spectrometry J. Michael Ramsey University of North Carolina at Chapel Hill, Chapel Hill, NC, US 15:50 – 16:20 PL7 Developments in the Design and Usage of High Performance Ion-Exchange Columns Paul Haddad Australian Centre for Research on Separation Science, Hobart, Tasmania, AU 16:20 – 16:40 PL8 High-Impact Liquid Chromatography Peter Schoenmakers University of Amsterdam, Amsterdam, NL 16:40 – 17:00 Closing Ceremonies (including Award Presentations) 17:00 – 18:00 Farewell Reception Location: First Floor restaurant

List of Posters

List of Abbreviations

BIOM Biomedical BIOP Biopharmaceutical BIOT Biotechnology CEFF Column efficiency CEMS CE-MS CEPH Capillary electrophoresis CHIP Chip-based separations CHIR Chiral separations CMTR Chemometrics COLS Columns ENVI Environmental FOOD Food FORC Forensic FUND Fundamentals GLYC Glycans HILC HILIC HRMS High-resolution MS INST Instrumentation LCLC LC×LC LCMS LC-MS principles LCSM LC-MS of small molecules LPDS Lipids MASS Mass spectrometry META Metabolomics MONO Monoliths MSDA MS data analysis NANO Nanomaterials PHAR Pharmaceutical POLY Polymers PROT Proteomics QTMS Quantitative MS RMEC Retention mechanisms SAMP Sample preparation SELE Selectivity SSFC Sub- and supercritical-fluid chromatography STPH Stationary phases

MO Presented on Monday TU Presented on Tuesday WE Presented on Wednesday

Biomedical BIOM01_MO Method Validation for the Analysis of Citrulline and Related Amino Acids in Plasma by Using the Last Generation Monolithic Column in RP-HPLC Mode Agustín Acquaviva1, Lilian Romero1, Cecilia Castells1, J. Manuel Herrero Martinez2 1LIDMA, La Plata, AR; 2Departamento de Química, Valencia, ES

BIOM02_TU Sensitive Analysis of 5-Nitroimidazoles by Cation-Selective-Exhaustive-Injection and Sweeping-Micellar-Electrokinetic-Chromatography in Untreated Clinical Samples Diego Airado-Rodríguez, Maykel Hernández-Mesa, Carmen Cruces-Blanco, Ana María García- Campaña Department Analytical Chemistry, University of Granada, Granada, ES

BIOM03_WE LC-MS Quantification of 6-Methylmercaptopurine to Determine Thiopurine S- Methyltransferase Activity in Red Blood Cells Joseph Bailey1, Molly Zaman1, Christopher Titman2, Alan Barnes2, Sally Benton1 1Clinical Biochemistry Department, Barts Health NHS Trust, London, GB; 2Shimadzu Corporation, UK, Milton Keynes, GB

BIOM04_MO Separation and Isolation of Pure and Active Antimicrobial Peptide of Bacteriocin Class Using HPLC for Modeling of Preparative Scale Separation Beata Furmanek-Blaszk1, Joanna Głazowska2, Daniel Jastrzębski2, Mariusz Jaszczołt2, Michał Laskowski2, Grzegorz Boczkaj2, Marian Kamiński2 1University of Gdańsk, Biology Faculty, Department of Microbiology, Gdansk, PL; 2Gdansk University of Technology, Gdansk, PL

BIOM05_TU Development of the Isolation of Whey Proteins Using High Performance Liquid Chromatography on Preparative Scale Mariusz Jaszczołt, Joanna Głazowska, Karol Kadlec, Grzegorz Boczkaj, Marian Kamiński Gdansk University of Technology, Gdansk, PL

BIOM06_WE Analytical Power of LC-UV-MS/MS in Drug Metabolism Studies: Phase I and II Gentamicin Metabolites Produced by Human Liver Microsomal Fractions Bogusław Buszewski1, Małgorzata Szultka1, Rafał Krzemiński2, Marek Jackowski2 1Nicolaus Copernicus University, Faculty of Chemistry, Toruń, PL; 2Nicolaus Copernicus University, Collegium Medicum, Bydgoszcz, PL

BIOM07_MO Ultra High Performance Liquid Chromatography and High Resolution Mass Spectrometric Detection in the Clinical Laboratory – Use of 2u Particle Packed Reversed Phase Columns Karin Cabrera, Michael Schulz, Anita Piper, Petra Lewits, Patrik Appleblad Merck KGaA, Darmstadt, DE

BIOM08_TU Study of Inhibitors of Abl1 Tyrosine Kinase by an Indirect Pulsed Electrochemical Detection Method Hui Chen, Erwin Adams, Ann Van Schepdael KU Leuven, Leuven, BE

BIOM09_WE Protein Unbound Pharmacokinetic Interaction of a Traditional Chinese Medical Formulation, Jia-Wei-Xiao-Yao-San, on 5-Fluorouracil in Rats Blood and Brain by Microdialysis Meng-Hsuan Chiang1, Lie-Chwen Lin2, Tung-Hu Tsai1 1National Yang-Ming University, Taipei, TW; 2National Research Institute of Chinese Medicine, Taipei, TW

BIOM10_MO A Simplified and Sensitive Strategy for the Determination of Plasma Total Homocysteine and Metabolic Related Vitamins B6, B9 and B12 Using MEPS-UHPLC- PDA Analysis Hugo Câmara, Jorge Pereira, José S. Câmara CQM, UMa, Funchal, PT 41

BIOM11_TU Aptamers Sieving for Protein, Bacteria and Cell Based on Capillary Electropohresis Feng Qu, Xinying Zhao, Xiaomei Zhou, Zhaoyang Meng, Beilei Lou School of Life Science, Beijing Instititu of Technology, Beijing, CN

BIOM12_WE Determination of Warfarin and Warfarin Alcohols in Saliva and Plasma by High Performance Liquid Chromatography with Fluorimetric Detection Silvia Ghimenti1, Tommaso Lomonaco1, Isabella Piga1, Denise Biagini1, Massimo Onor2, Fabio Di Francesco1, Aldo Paolicchi3, Lucia Ruocco4, Giovanni Pellegrini4, Maria Giovanna Trivella5, Roger Fuoco1 1University of Pisa, Dept of Chemistry and Ind. Chemistry, Pisa, IT; 2Institute of Chemistry of Organometallic Compounds, CNR, Pisa, IT; 3University of Pisa, Dept of Translational Research and New Technologies in Medicine and Surgery, Pisa, IT; 4Chemical-Clinical Analysis Laboratory, AOUP, Pisa, IT; 5Institute of Clinical Physiology, CNR, Pisa, IT

BIOM13_MO Development and Validation of UHPLC-MS/MS Method for the Quantification of Cortisol and 6β-Hydroxycortisol in Human Urine as Biomarker of CYP3A Activity Kim Hor Daryl Hee, Lawrence Soon-U Lee National University of Singapore, Singapore, SG

BIOM14_TU Rational Design and Affinity Evaluation of Polyvalent Peptide Probes for Tumor Biomarker Targeting Yanyan Huang, Rui Zhao Institute of Chemistry, Chinese Academy of Sciences, Beijing, CN

BIOM15_WE Developing High Throughput 2D-LC-MS/MS Method for Quantification of Vitamin D Which Overcomes Challenges of Chemiluminescent Immunoassay Siji Joseph1, Sreekala K.N2, Sudha Rajagopalan1, Anura Kurpad2, Amit Mandal2 1Agilent Technologies, Bangalore, IN; 2St. John’s Research Institute, Bangalore, IN

BIOM16_MO Biomarkers Determination in Exhaled Breath Condensate, Plasma and Urine by Liquid Chromatography–Tandem Mass Spectrometry with Simple Sample Preparation Agata Kot-Wasik, Monika Janicka, Andrzej Wasik, Jacek Namieśnik Gdańsk University of Technology, Gdańsk, PL

BIOM17_TU Development of New HPLC Method for Determination of Retinol, Alpha- and Gamma- Tocopherol Using High Resolution Monolithic Column for Bioanalysis Barbora Kučerová1, Lenka Krčmová1, Dagmar Solichová2, Petr Solich1 1Charles University, Faculty of Pharmacy, Hradec Králové, CZ; 2University Hospital, III. Internal Gerontometabolic Clinic, Hradec Králové, CZ

BIOM18_WE Assessment of Urinary Biomarkers of Oxidative Stress in Cancer and Cardiovascular Pathologies Using a Semi-Automatic Micro-Extraction Technique Combined with UHPLC-PDA System Berta Mendes1, Pedro Silva1, Jorge Pereira1, Fernando Aveiro2, Isabel Mendonça1, José S. Câmara1 1CQM, UMa, Funchal, PT; 2SESARAM, Hospital Dr. Nélio Mendonça, Funchal, PT

BIOM19_MO Rapid Quantitation of Five Immunosuppresants from Body Fluids Siji Joseph, Sreelakshmy Menon Agilent Technologies, Bangalore, IN

BIOM20_TU Exploring the Potential of Microextraction by Packed Sorbent (MEPS) Combined with Ultrahigh Pressure Liquid Chromatography to Quantify the Urinary Leukotriene B4 Levels in Asthmatic Patients Rosa Perestrelo, Catarina L. Silva, Jorge Pereira, José S. Câmara CQM, UMa, Funchal, PT

BIOM21_WE Development and Validation of a Chromatographic Method for Determination of Allopurinol and its Derivatives Mónica Raviolo1, Margarita Briñón1, J. Peris-Vicente2, Samuel Carda-Broch2, Enrique Ochoa- Aranda3, Josep Esteve-Romero2 1Universidad Nacional Córdoba, Córdoba, AR; 2University Jaume I, Castelló, ES; 3Hospital Provincial, Castelló, ES

BIOM22_MO Determination of Tamoxifen and its Main Metabolits in Plasma Samples from Breast Cancer Patients Enrique Ochoa-Aranda1, Juan Peris-Vicente2, Samuel Carda-Broch2, Mónica-Ana Raviolo3, Josep Esteve-Romero2 1Hospital Provincial, Castelló, ES; 2University Jaume I, Castelló, ES; 3Universidad Nacional Córdoba, Córdoba, ES

BIOM23_TU Chromatographic Determination of Lipophilicity of Potential Antitrypanosomal Compounds Mónica Raviolo1, Margarita Briñón1, María S. Gualdesi1, Juan Peris-Vicente2, Samuel Carda- Broch2, Josep Esteve-Romero2 1Universidad Nacional Córdoba, Córdoba, AR; 2University Jaume I, Castelló, ES

BIOM24_WE Relationships between the Dose of Tamoxifen and CYP2D6 Phenotype Enrique Ochoa-Aranda1, Eduardo Martínez-Dueñas1, Teresa Ferrer-Magdalena1, Juan Peris- Vicente2, Samuel Carda-Broch2, Josep Esteve-Romero2, Mónica-Ana Raviolo3 1Hospital Provincial, Castelló, ES; 2University Jaume I, Castelló, ES; 3Universidad Nacional Córdoba, Córdoba, AR

BIOM25_MO Hospitalary Monitorization in Blood of an Highly Active Antiretroviral Therapy Regime Juan Peris-Vicente1, Inmaculada Casas-Breva2, Samuel Carda-Broch1, Mónica Villarreal-Traver1, David Fabregat-Safont1, Noelia Fuentes-Navarro1, Ester Peris-García1, Josep Esteve-Romero1 1University Jaume I, Castelló, ES; 2Hospital la Plana, Castelló, ES

BIOM26_TU Simultaneous Determination of Three Traditional and Two Novel Antiepileptic Drugs Using Micellar Liquid Chromatography Swati Dubey1, Sudhir Kumar Shukla2, Abhilasha Durgbanshi3, Josep Esteve-Romero4, Devasish Bose1, Juan Peris-Vicente4, Samuel Carda-Broch4 1Dr. H.S.Gour University, Sagar, IN; 2Central Forensic Science Laboratory (CFSL), Chandigarh, IN 3Regional Forensic Science Laboratory, Dharamshala, IN; 4University Jaume I, Castelló, ES

BIOM27_WE Determination of Glutathione and Glutathione Disulfide in Mouse Brain Using High Performance Liquid Chromatography with Post-Column Chemiluminescence Detection Zoe M. Smith1, Jessica M. Terry1, Laura J. Gray1, Dean J. Wright2, Neil W. Barnett1, Paul S. Francis1 1Deakin University, Geelong, VIC, AU; 2University of Melbourne, Melbourne, VIC, AU

BIOM28_MO The Use of Linear Ion Trap for Qualitative and Quantitative Analysis of Phytochemicals in Ginseng Samples Andrey Stavrianidi, Igor Rodin, Arkady Braun, Oleg Shpigun Moscow State University, Moscow, RU

BIOM29_TU UHPLC with Electrochemical Detection for the Quantitative Determination of Picomolar Concentrations of Monoamines in Microdialysis Samples Jolien Van Schoors1, Hendrik-Jan Brouwer2, Katrien Maes1, Lusi Van Heerwaarden2, Nico Reinhoud2, Yvette Michotte1, Ann Van Eeckhaut1 1Vrije Universiteit Brussel, Jette, Brussels, BE; 2Antec BV, Zoeterwoude, NL

BIOM30_WE Pharmacokinetics of Alkaloids Isolated from a Traditional Antimalarial Decoction of Argemone Mexicana L. in CD1 Mice and in Humans Amina Haouala1, Jacques Falquet2, Bertrand Graz2, Claudia Simoes-Pires1, Issaka Sagara3, Ogobara K. Doumbo3, Rokia Sanogo4, Santiago Ferrer5, Adolfo Garcia5, Xavier Ding6, Muriel Cuendet1, Philippe Christen1 1Geneva University, Geneva, CH; 2Lausanne University, Lausanne, CH; 3Malaria Research and Training Center, Bamako, ML; 4Département De Médecine Traditionnelle, Bamako, ML; 5GSK, Madrid, ES; 6Medicines for Malaria Venture, Geneva, CH

BIOM31_MO Neonatal Screening Using Shimadzu LCMS-8040 Giancarlo La Marca1, Sabrina Malvagia2, Stefano Zaza3 1Mass Spectrometry and Pharmacology Laboratory, A. Meyer Chil, Florence, IT; 2Mass Spectrometry and Pharmacology Laboratory, A. Meyer Children’s Hospital Pediatric Neurology Unit and Laboratories, University of Florence, Florence, IT; 3Shimadzu Italia, Milan, IT

Biopharmaceutical BIOP01_MO The Role of Internal Standards in High-Sensitivity Protein Quantifications with LC- MS/MS Kees Bronsema1, Rainer Bischoff1, Nico van de Merbel2 1University of Groningen, Groningen, NL; 2PRA-International EDS laboratory, Assen, NL

BIOP02_TU Multidimensional LC/UV-BChE-ICER Method for Ligand Screening Carmen Lucia Cardoso Universidade de São Paulo-USP, Ribeirão Preto- SP, BR

BIOP03_WE Metallic Derivatives as Potential Cholinesterase Inhibitors: Comparative Studies Using On-Flow Inhibitors Screening Assay Adriana Ferreira Lopes Vilela1, Bárbara Mammana Frugeri1, Kenia Lourenço Vanzolini2, André Lúcio Franceschini Sarriá2, João Baptista Fernandes2, Quezia Bezerra Cass2, Carmen Lucia Cardoso1 1Universidade de São Paulo-USP, Ribeirão Preto- SP, BR; 2Universidade Federal de São Carlos- UFSCar, São Carlos - SP, BR

BIOP04_MO Orthogonal Peptide Mapping Analysis of Erythropoietin (EPO) Protein by HILIC and Reversed-Phase Chromatography Andrew Coffey1, Alex Zhu2, James Martosella2, Phu Duong2, Maureen Joseph2 1Agilent Technologies UK Ltd, Church Stretton, GB; 2Agilent Technologies Inc, Wilmington, US

BIOP05_TU Reversed-Phase LC/MS Characterization of Glycosylation in the Fc Region of a Therapeutic Recombinant Monoclonal Antibody (mAb) Andrew Coffey1, Alex Zhu2, James Martosella2, Phu Duong2, Maureen Joseph2 1Agilent Technologies UK Ltd, Church Stretton, GB; 2Agilent Technologies Inc, Wilmington, US

BIOP06_WE The Impact of Amino Acids on Analytical SEC of mAb Aggregates Judith Vajda, Werner Conze, Egbert Müller Tosoh Bioscience, Stuttgart, DE

BIOP07_MO Fast and Robust Direct Infusion ESI-MS Method for the Characterization of PEGylated Proteins Ines C. Forstenlehner1, Johann Holzmann2, Wolfgang Wieder2, Hansjörg Toll2, Christian G. Huber1 1University of Salzburg, Salzburg, AT; 2Sandoz, Kundl, AT

BIOP08_TU Considerations in the HPLC Analysis of Biomolecules Sandra Hanson, Mark Woodruff Fortis Technologies Ltd, Neston, GB

BIOP09_WE Cation-Exchange Chromatography with Conductivity Detection for the Analysis of Cations in Human Saliva Kyung Suk Hwang1, Yooseok Kim2, Su Min Yoo3, Chang Seon Yoo2, Ji Eun Kim2, Ha Jeong Kwon2 1Department of Dental Technology, Shinheung University, Uijeongbu, Kyunggido, KR; 2Department of Preventive Social Dentistry School of Dentistry, Kyung Hee University, Seoul, KR; 3Department of Dental Hygiene, Dong-Woo College, Sokcho, KR

BIOP10_MO Analytical Challenges in Analysis of Peptides & Proteins in Oral Solide Dose Formulations Babak Jamali Novo Nordisk, Maaloev, DK

BIOP11_TU High Resolution Analysis of High Molecular Weight Components Contained within Igg Using the Latest Silica-Based SEC Columns Yasutoshi Kawai, Hiroyuki Yamasaki, Shigeru Nakatani Tosoh corporation, Shunan, Yamaguchi, JP

BIOP12_WE Pharmacokinetics and Bioavailability of Epimedin C in Rat after Oral Administration of Herba Epimedii Extract Measured by Liquid Chromatography Coupled to Tandem Mass Spectrometry Chia-Jung Lee1, Thomas Y. Hsueh1, Wan-Ning Tseng1, Lie-Chwen Lin2, Tung-Hu Tsai1 1National Yang-Ming University, Taipei, TW; 2National Research Institute of Chinese Medicine, Taipei, TW

BIOP13_MO Determination of Oseltamivir and Oseltamivir Carboxylic Acid in Rat Brain after Oseltamivir Intravenous Administration and its Neurochemical Interaction Chia-Chun Lin1,2, Jiin-Cherng Yen1, Yu-Tse Wu2, Lie-Chwen Lin2,3, Tung-Hu Tsai2,4 1Institute of Pharmacology, 2Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei, TW

BIOP14_TU Development of a Cation-Exchange Ph Gradient Separation Platform Shanhua Lin, Wim Decrop, Julia Baek, Srinivasa Rao, Yury Agroskin, Chris Pohl Thermo Fisher Scientific, Sunnyvale, US

BIOP15_WE High-Speed, High Resolution Oligonucleotide Separations Using Small Particle Anion-Exchangers Shanhua Lin1, J. R. Thayer1, Ken Cook2, Sinivasa Rao1 1Thermo Scientific, Sunnyvale, CA, US; 2Thermo Scientific, Hemel Hempstead, GB

BIOP16_MO Advantages of Small Particle High Resolution Separation Media for Monoclonal Antibody Analysis Srinivasa Rao, Yuanxue Hou, Julia Baek, Shanhua Lin, Hongmin Zhang, Yury Agroskin, Chris Pohl Thermo Fisher Scientific, Sunnyvale, US

BIOP17_TU LC-MS for the Assignment of Cys-Cys Disulfide Connectivities in Venom Proteins Miriam Goyder Analytical Chemistry , Institute of Life Technologies, HES-SO, Valais-Wallis, CH

BIOP18_WE Elimination Or Isolation of Phospholipids from Biological Matrices before HPLC/MS Analysis Using Zirconia-Based Adsorbents Frank Michel1, Craig R. Aurand2, Xiaoning Lu2, David S. Bell2 1Sigma-Aldrich, Taufkirchen, DE; 2Supelco/Sigma-Aldrich, Bellefonte, US

BIOP19_MO Fabrication of Large-Sized Silica Monolith Exceeding 1000 Ml with High Structural Homogeneity and the Chromatographic Performances Riichi Miyamoto1, Yukiko Ando2, Chie Kurusu2, Hong-zhi Bai2, Kazuki Nakanishi1, Masamichi Ippommatsu3 1Graduate School of Science, Kyoto University, Kyoto, JP; 2Renaissance Energy Investment Co., Ltd., Kyoto, JP; 3New Industry Creation Hatchery Center, Tohoku University, Miyagi, JP

BIOP20_TU QA/QC of Monoclonal Antibodies: Charge Variants and Disulfide Linkage Analysis Sundaram Palaniswamy, Ravindra Gudihal, Siji Joseph Agilent Technologies Pvt Ltd, Bangalore, IN

BIOP21_WE Purification and Characterization of Lysozyme Modified with Poly Ethylene Glycol (PEG) Sundaram Palaniswamy1, Lakshmi NS2, Ravindra Gudihal1 1Agilent Technologies India, Bangalore, IN; 2Amrita University, Kollam, IN

BIOP22_MO Use of Entrapment and High-Performance Affinity Chromatography to Compare the Binding of Drugs and Site-Specific Probes with Normal and Glycated Human Serum Albumin Abby Jackson, Jeanethe Anguizola, Erika Pfaunmiller, David Hage University of Nebraska-Lincoln, Lincoln, US

BIOP23_TU Soy Isoflavones: An Automated and Validated UHPLC–MS/MS Method for the Determination of the Phase II Metabolites in Blood Plasma and Urine Sebastian Soukup1, Sabine E. Kulling2 1Max Rubner-Institut,, Karlsruhe, DE; 2Max Rubner-Institut, Karlsruhe, DE

BIOP24_WE Global Analytical Strategy to Characterize Drug-Plasma Protein Interactions: from High Throughput to In-Depth Analysis. Karine Vuignier, Jean-Luc Veuthey, Pierre-Alain Carrupt, Julie Schappler University of Geneva, Switzerland, Geneva, CH

BIOP25_MO Silica-Based Monolithic Capillary Columns Modified by Liposomes for Characterization of Analyte-Liposome Interactions by Capillary Liquid Chromatography Susanne Wiedmer1, Dana Moravcová2, Josef Planeta2 1University of Helsinki, Department of Chemistry, Helsinki, FI; 2Institute of Analytical Chemistry of the ASCR, Brno, CZ

BIOP26_TU High-Performance Liquid Chromatography (HPLC) in Non-Clinical Toxicology Research of Pharmaceutical Proteins Shishan Zhao Charles River Laboratories, Montreal, CA

BIOP27_WE Development of an Accurate HP-SEC Method for the Quantification of Polio Virus in (Partially) Purified In-Process Samples. Jonathan Knibbe, Annemiek Y. W. Verwilligen, Pascal de Raadt, Harold H.J. Backus Crucell Holland BV, Leiden, NL

Biotechnology BIOT01_MO Orthogonal Ion-Pairing Reversed Phase Liquid Chromatography Purification of TaqMan® Probes with Bulky Fluorophores Concordio Anacleto, Nancy Schoenbrunner Roche Molecular Diagnostics, Pleasanton, CA, US

BIOT02_TU Linear and Non Linear Zonal Bioaffinity Chromatography: A Tool for Ligand Characterization Quezia Cass1, K.L. Vanzolini1, L.C.C. Vieira1, A.G. Corrêa2, C.L. Cardoso2, R. Moaddel3 1Universidade Federal de São Carlos, São Carlos - SP, BR; 2Departamento de Química, USP- Ribeirão Preto, BR; 3Biomedical Research Center, NIA-NIH, Baltimore, MD, US

BIOT03_WE A Multidimensional Chromatography Method for on Line Cathepsin D Ligands Screening in Natural Products Vivian Cornelio1, Marcela Moraes2, Quezia Cass1, Paulo Cezar Vieira1 1Department of Chemistry, University of Sao Carlos, Sao Carlos - SP, BR; 2Instituto de Bioquímica Médica, Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro - RJ, BR

BIOT04_MO High Performance Anion Exchange Chromatography (HPAEC)-Mass Spectrometry (MS): Analysis of Complex Mixtures of Oligosaccharides in Biomass Hydrolysates Leon Coulier, Wibo van Scheppingen, Rob van der Hoeven, Jort Gerritsma DSM Biotechnology Center, Delft, NL

BIOT05_TU Automated, Unattended Purification of Beta-Galactosidase, Trypsines, and Proteases with a Single, High Pressure LC Purification System Markus Fuchs Wissenschaftliche Geraetebau Dr. Ing. H. KNAUER GmbH, Berlin, DE

BIOT06_WE Confirmation of the Nature of Solid Residue from Beff Tallow Biodiesel Used as Fuel by LC-MS-IT-TOF Paulo Pivesso Petroleum, Natural Gas and Biofuels National Agency Brazil, Brasilia / DF, BR

BIOT07_MO Direct LC-MS Analysis of Reduced and Oxidized Cysteine and Glutathione in Microbiological Sample Matrices Pieter Stam, Ilco Boogers, Alexander L.L. Duchateau DSM Biotechnology Center, Delft, NL

BIOT08_TU Process of Extration, Purification, Isolation of Epiisopiloturine Alkaloid and its Antiparasitic Activity against Schistosoma Mansoni Leiz Véras1, Maria Adelaide Guimarães1, Marianne Vieira1, Yuri Campelo1, David Lima1, Paulo Silva2, Luciene Vasconcelos2, Markus Godejohann3, Josué Moraes4, José Roberto Leite1 1Federal of Piauí University, Parnaíba - PI, BR; 2Anidro do Brasil, Parnaíba - PI, BR; 3Bruker BioSpin, Rheinstetten, DE; 4Vigilância Sanitária em Saúde, São Paulo - SP, BR

BIOT09_WE Development of Robust Platform High-Throughput Ion Exchange Chromatography for Charge Heterogeneity Analysis of Therapeutic Proteins Jennifer Wang, Tony Moreno, Dan McDonald, Dell Farnan Genentech, Inc., South San Francisco, US

BIOT10_MO High Performance Liquid Chromatography Detection of Products Formed by Recombinant Carotenoid Biosynthesis Pathways Astrid Wirtz, Anita Loeschcke, Annette Markert, Susanne Wilhelm, Frank Rosenau, Karl-Erich Jaeger, Thomas Drepper Institut für Molekulare Enzymtechnologie, Juelich, DE

BIOT11_TU Novel Sample Preparation Strategy for High Efficient Integral Membrane Protein Analysis Qun Zhao, Lihua Zhang, Yukui Zhang Dalian institute of chemical physics, CAS, Dalian, CN

BIOT12_WE Analysis of Mannosylerythritol Lipids by Normal Phase Liquid Chromatography Atmospheric Pressure Photo Ionization Mass Spectrometry Wilma du Chatinier, Hans-Gerd Janssen Unilever R&D Vlaardingen, Vlaardingen, NL

Column Efficiency CEFF01_MO Evaluation of Fused – Core Silica Stationary Phases for the Chromatographic Analysis of Different Pharmaceutical Drugs Amal Ammar1, Peter Surmann2 1Faculty of Pharmacy, University of Tripoli, Tripoli, LY; 2Faculty of Pharmacy, Free University, Berlin, DE

CEFF02_TU Chromatographic Method Development Using a Design Approach for the Quantification of Metabolites in Ilex Paraguariensi Flávio Beltrame1, Rodrigo M. C. Pinto1, Bruna M. Lemes1, Fernanda M. Kreutz1, Acácio A. F. Zielinski1, Traudi Klein1, Airton V. Pereira1, Tiago Campos2, Fernando de Paula2 1State University of Ponta Grossa, Ponta Grossa, BR; 2Waters Technologies - Brazil, São Paulo, BR

CEFF03_WE Comparison of the Separation Performance Limits of Small and Large Fully Porous and Superficially Porous Particles Yoachim Vanderheyden1, Deirdre Cabooter2, Gert Desmet1, Ken Broeckhoven1 1Vrije Universiteit Brussel, Brussels, BE; 2K.U.Leuven, Leuven, BE

CEFF04_MO High Speed Separation of Nucleosides in UHPLC Ken Butchart, Neil Mcgrath Fortis Technologies Ltd, Neston, GB

CEFF05_TU Towards a Generic Variable-Length Method Development Strategy for Samples with a Large Variety in Polarity. Pharmaceuticals in Waste Water: a Case Study Deirdre Cabooter1, Yves Loyson1, François Lestremau2, Monika Dittmann3, Kris Wolfs1, Gert Desmet4 1K.U.Leuven, Leuven, BE; 2INERIS, Verneuil-en-Halatte, FR; 3Agilent Technologies, Waldbronn, DE; 4V.U.B., Brussel, BE

CEFF06_WE Fused Core Particles – An Assessment of their Practical Daily Use within the Pharmaceutical Industry Barbara Channer1, Sabrina Lisandrin1, Mel Euerby2 1Actelion Pharmaceuticals Ltd., Allschwil, CH; 2Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, GB

CEFF07_MO Development of an NARP-UHPLC-MS Method for the Analysis of Milk Triacylglycerols Marco Beccaria, Giuseppe Sullini, Paola Donato, Carla Ragonese, Marina Russo, Francesco Cacciola, Paola Dugo, Luigi Mondello University of Messina, Messina, IT

CEFF08_TU Synthesis of Superficially Porous Particles via Coacervation and the Benefits of Using this Method of Particle Manufacture Wu Chen, Ta-Chen Wei, William Long Agilent Technologies, Wilmington, Delaware, US

CEFF09_WE Speed and Resolution with 5-Micron Superficially Porous Particles Joseph DeStefano1, Joseph Kirkland1, Stephanie Schuster1, William Johnson1, Thomas Waeghe2 1Advanced Materials Technolgy, Inc., Wilmington, Delaware, US; 2Mac-Mod Analytical, Inc., Chadds Ford, Pennsylvania, US

CEFF10_MO A Simple Multi-Residue Method for Analysis and Quantification of Fluoroquinolones Antibiotics in Wastewater and Surface Water by LC-MS/MS Marina Denadai, Quezia Cass Universidade Federal de São Carlos - UFSCar, SÃO CARLOS, BR

CEFF11_TU Evaluation of Ultra-High Performance Size Exclusion Chromatography for Protein Aggregates, Using Sub-2 µm Particles Szabolcs Fekete1, Katalin Ganzler2, Davy Guillarme1 1University of Geneva, Geneva, CH; 2Gedeon Richter Plc, Budapest, HU

CEFF12_WE Development of an SPE-LC/MS/MS Method for Simultaneous Quantification of Intact Human Insulin and 5 Analogs in Human Plasma Kenneth Fountain1, Erin Chambers1, Norman Smith2, Cristina Legido-Quigley2 1Waters Corporation, Milford, MA, US; 2Kings College London, London, GB

CEFF13_MO The Development and Application of new Fused-Core HPLC Particle Parmar Gaurang1, Wayne Way1, Richard Henry1, Jens Boertz2 1Sigma-Aldrich, Bellefonte, US; 2Sigma-Aldrich, Buchs, CH

CEFF14_TU Methods for Improving Laboratory Productivity by Reducing UHPLC and LC/MS System Downtime Maureen Joseph1, Anne Mack1, William Long1, Jason Link1, Norwin von Doehren2, Wilroy Bennen2, Andrew Coffey3 1Agilent Technologies Inc, Wilmington, US; 2Agilent Technologies BV, Middelburg, NL; 3Agilent Technologies UK Ltd, Church Stretton, GB

CEFF15_WE Morphology Transport Relations for Packed Beds: From Metric Properties to Structural Descriptors of Diffusion and Hydrodynamic Dispersion Siarhei Khirevich, Stefan Bruns, Alexandra Höltzel, Ulrich Tallarek Philipps-Universität Marburg, Marburg, DE

CEFF16_MO A Challenge for Visualization of Kinetics on Separation Parameters in High Performance Liquid Chromatography Hiroshi Kobayashi Shinwa Chemical Industries Ltd., Kyoto, JP

CEFF17_TU Comparison of Measured Peak Capacity Using HPLC, UHPLC and LC´LC Systems for Analysis of Complex Samples of PAH Biodegradation Products Mie Kristiansen, Jan Christensen, Rune Græsbøll, Esther Boll, Nikoline Nielsen University of Copenhagen, Frederiksberg, DK

CEFF18_WE Trends in HPLC Column Technology and their Application: From Small Particles and Long Columns Stefan Lamotte BASF SE, Ludwigshafen, DE

CEFF19_MO Synthesis of Stationary Phase for Reverse-Phase High-Performance Liquid Chromatography Based on Thermal Immobilization of PMDTS- (polymethyltetradecylsiloxane) onto Aluminized Silica Renata Nome, Carol Collins UNICAMP, Campinas, BR

CEFF20_TU HPLC Determination of Agomelatine in Tablets Using Different Types of Stationary Phases Mehmet Emre Özdemirhan, Nafiz Öncü Can Anadolu University, Eskisehir, TR

CEFF21_WE Advances in Column Technology for Pharmaceutical Applications Mia Summers, Kenneth Berthelette, Kenneth Fountain Waters Corp, Milford, MA, US

CEFF22_MO Rapid HPLC Method for Estimation of Catechin from Albizia Lebbeck Bark Extracts Pratima Tatke1, Sonal Desai1, S.Y. Gabhe2, Vinay Gaitonde3 1C. U. Shah College of Pharmacy, Mumbai, MS, IN; 2Poona College of Pharmacy, Pune, MS, IN; 3Biotech India Pvt. Ltd., Mumbai, MS, IN 51

CEFF23_TU Packing Efficient Capillary Columns with Sub-2 Micron Superficially Porous Particles James Treadway, James Jorgenson University of North Carolina - Chapel Hill, Chapel Hill, NC, US

CEFF24_WE UHPLC Analysis of Biomolecules Mark Woodruff, Neil McGrath Fortis Technologies Ltd, Neston, GB

CEFF25_MO Use of UPLC as a Fast and Efficient Approach to Determine the Log Kow Martien van der Meijden, Rob Beks, Erik Baltussen, Theo Noij WIL Research Europe, 's-Hertogenbosch, NL

CEFF26_TU Advances in Particle and Packing Technology for Higher Efficiency UltraPerformance LC Columns Thomas Walter, Kevin Wyndham, Steve Shiner, Daniel Walsh, Michael Savaria, Pamela Iraneta, Jonathan Turner, Cheryl Boissel, Scott McCall Waters Corporation, Milford, MA, US

CEFF27_WE Development and Validation of New HPLC Method for Fast Analysis and High Separation Efficiency with Low Back Pressure Amal Ammar1, Mohamed El-Attug1, Anisa Elhamili1, Peter Surmann2 1Faculty of Pharmacy, University of Tripoli, Tripoli, LY; 2Faculty of Pharmacy, Free University, Berlin, DE

CEFF28_MO How to Optimise Your UHPLC Performance – Connect Properly! Ken Butchart, Neil Mcgrath Fortis Technologies Ltd, Neston, GB

CEFF29_TU Experimental Investigation of the Possibilities of Ultra-Performant Chromatographic Columns Ken Broeckhoven, Yoachim Vanderheyden, Gert Desmet Vrije Universiteit Brussel, Brussel, BE

CEFF30_WE Characterization of a New 1.3 µm Core-Shell Material Szabolcs Fekete1, Tivadar Farkas2, Davy Guillarme1 1University of Geneva, Geneva, CH; 2Phenomenex, Torrance, US

CEFF31_MO Optimal Method Development Utilizing Modern UHPLC Columns via a Quality–By- Design (Qbd) Methodology Maureen Joseph1, Jason Link1, William Long1, Andrew Coffey2 1Agilent Technologies Inc, Wilmington, US; 2Agilent Technologies UK Ltd, Church Stretton, GB

CEFF32_TU Performance of High Efficiency Columns and the Impact of System Dispersion Jonathan Turner, Pamela Iraneta, Bonnie Alden, Daniel Walsh, James Cook, Steven Shiner, Michael Savaria, Kevin Wyndham, Thomas Walter Waters Corp, Milford, Ma, US

CE-MS CEMS01_MO CE-UV/MALDI-MS: A New Platform Using an Integrative Matrix Delivery System for Proteomics and Intact Proteins Applications Michael Biacchi, Jasmine Hertzog, Cyril Adolf, Yannis-Nicolas Francois, Emmanuelle Leize- Wagner LSMIS, UMR 7140, University of Strasbourg, Strasbourg, FR

CEMS02_TU Development of an Immunoaffinity Sorbent with Fab’ Antibody Fragments for the Analysis of Neuropeptides by IA-SPE-CE-MS Fernando Benavente, Silvia Medina-Casanellas, José Barbosa, Victoria Sanz-Nebot Department of Analytical Chemistry, University of Barcelona, Barcelona, ES

CEMS03_WE Analysis of Glycopeptide Glycoforms of Human Transferrin by Capillary Liquid Chromatography-Mass Spectrometry. Application to Alcoholism Diagnosis Fernando Benavente, Albert Barrroso, Estela Giménez, José Barbosa, Victoria Sanz-Nebot Department of Analytical Chemistry, University of Barcelona, Barcelona, ES

CEMS04_MO Capillary Electrophoresis-Triple-Quadrupole Mass Spectrometry Based Peptide Quantification Suresh Babu C.V.1, Martin Greiner2, Sundaram Palaniswamy3 1Agilent Technologies India Pvt. Ltd., Bangalore, IN; 2Agilent Technologies R&D and Mktg. GmbH & Co.KG,, Waldbronn, DE; 3Agilent Technologies, Bangalore, IN

CEMS05_TU Analysis of the sPRG PTM Peptide Standard Using Sheathless Capillary Electrophoresis Electrospray Mass Spectrometry (CESI-MS) Klaus Faserl, Bettina Sarg, Herbert Lindner Innsbruck Medical University, Innsbruck, AT

CEMS06_WE A Next Generation Mass Spectrometry Platform for the Rapid Identification of (Multi-)Drug Resistant Gram-Negative Bacteria Frank Fleurbaaij Leiden University Medical Center, Leiden, NL

CEMS07_MO Characterization of Nitrated Allergens by CZE-ESI-TOF MS Sergey Gusenkov, Chloé Ackaert, Gertie Oostingh, Hanno Stutz University Salzburg, Salzburg, AT

CEMS08_TU Ultra-Low Flow CE-ESI-MS as an Analysis Tool for Post Translational Modifications Anton Heemskerk1, Bart Schoenmaker1, Jean-Marc Busnel2, Paul Hensbergen1, Manfred Wuhrer1, André M. Deelder1, Oleg A. Mayboroda1 1Leiden University Medical Center, Leiden, NL; 2Beckman Coulter Inc., Brea, CA, US

CEMS09_WE Online Coupling of Capillary Electrophoresis with Direct Analysis in Real Time Mass Spectrometry Huwei Liu, Cuilan Chang, Yu Bai Peking University, Beijing, CN

CEMS10_MO Sensitivity Enhancement in Capillary Electrophoresis–Mass Spectrometry Using LVSEP. 2 Hiroya Ota1, Takayuki Kawai2, Kenji Sueyoshi3, Takuya Kubo1, Fumihiko Kitagawa4, Koji Otsuka1 1Kyoto University, Kyoto, JP; 2National Institute of Advanced Industrial Science and Technology, Osaka, JP; 3Osaka Prefecture University, Osaka, JP; 4Hirosaki University, Hirosaki, JP

CEMS11_TU Investigation of the Oxidative Metabolism of the Alkaloid Harmane by Electrochemistry, Non-Aqueous Capillary Electrophoresis and Mass Spectrometry Jörg Roscher, Oliver Hachmöller, Jana Happe, Uwe Karst University of Münster, Münster, DE

CEMS12_WE Electrophoretic and Mass Spectrometric Analysis of Bacterial Endotoxins Viktor Sándor1, Dalma Scheffer1, Béla Kocsis2, Ferenc Kilár3, Anikó Kilár1, Ágnes Dörnyei3 1Dept. of Anal. and Envir. Chem., University of Pécs, Pécs, HU; 2Dept. of Med. Microb. and Immun., University of Pécs, Pécs, HU; 3Inst. of Bioanalysis, University of Pécs, Pécs, HU

CEMS13_MO Determination of Harmala Alkaloids in Edible Algae by Capillary Electrophoresis Mass Spectrometry Marcos Tascon1, Leonardo Gagliardi1, Fernando Benavente2, Jose Barbosa2, Victoria Sanz- Nebot2 1Universidad Nacional de La Plata, La Plata, AR; 2Universidad de Barcelona, Barcelona, ES

CEMS14_TU Detemination of Eight Fluoroquinolones in Domestic Sewage by Off‐Line Immunoaffnity Solid‐Phase Extraction Coupled with Electrospray Ionization Mass Spectroscopy Xin-Xiang Zhang1, Deng Yan1, Zhou Yinglin1, Zhang Xiao-Hui1, Li Peiwu2 1Peking University, Beijing, CN; 2the Oil Crops Research Institute (OCRI) of the Chinese Academy of Agricultural Sciences (CAAS), Wuhan, CN

Capillary Electrophoresis CEPH01_MO The Use of Discontinuous Buffers in Capillary Electrophoresis Analysis of Biopharmaceuticals Hytham Ahmed1, Wael Ebied2 1Faculty of Pharmacy, Damanhour University, Damanhour, EG; 2SEDICO Pharmaceutical Company, 6th October, EG

CEPH02_TU Off-Line Comprehensive IP-RPLC X CE and IEX-LC X CE for Oligonucleotide Impurity Mapping Piotr Alvarez, Frederic Lynen Ghent University, Gent, BE

CEPH03_WE Application of Various On-Capillary Stacking Techniques for Determination of Homocysteine-Thiolactone Kamila Borowczyk, Paulina Furmaniak, Paweł Kubalczyk, Rafał Głowacki University of Lodz, Faculty of Chemistry, Dep. Env. Chem., Lodz, PL

CEPH04_MO Accelerating Drug Discovery: Electrophoretic Front-End Library Screening for Biological Relevance Christa Colyer1, Kathryn Riley1, Shingo Saito2 1Wake Forest University, Winston-Salem, NC, US; 2Saitama University, Saitama, JP

CEPH05_TU Interinstrumental Transfer of a Chiral CE Method Using Precision Studies and Robustness Testing Data Bart De Cock, Birgit De Meuter, Bieke Dejaegher, Debby Mangelings, Yvan Vander Heyden Vrij universiteit Brussel, Jette, BE

CEPH06_WE Kinetic Performance Evaluation and Perspectives of Packed Column Capillary Electrochromatography Seppe De Smet, Frédéric Lynen Ugent, Gent, BE

CEPH07_MO Hydrophilic Interaction Capillary Electrochromatography (HICEC) Applied in Packed and Open-Tubular Capillaries Seppe De Smet, Frédéric Lynen Ugent, Gent, BE

CEPH08_TU New Developments for In-Situ Electrokinetic Preconcentration Techniques for Trace Analysis in Capillary Electrophoresis Nathalie Delaunay, Philippe Anres, Pierre Gareil PECSA, Chimie ParisTech, Paris, FR

CEPH09_WE Capillary Zone Electrophoresis for the Characterisation and Separation of Detonation Nanodiamond Emer Duffy, Dimitar Mitev, Pavel N. Nesterenko, Brett Paull Australian Centre for Research on Separation Science, Hobart, TAS, AU

CEPH10_MO Sweeping as a Multistep Enrichment Procedure in Cyclodextrin-Modified Micellar Electrokinetic Chromatography of Hydrophobic Basic Analytes: Application to Pharmaceuticals Mohamed El-Awady1, Fathalla Belal2, Ute Pyell1 1University of Marburg, Department of Chemistry, Marbrug, DE; 2University of Mansoura, Faculty of Pharmacy, Mansoura, EG

CEPH11_TU Enhancement of Coating Stability for Capillary Zone Electrophoresis of Proteins in DDAB Modified Surfaces Anisa Elhamili1, Amal Ammar1, Mohamed El-attug1, Shaban Saad1, Douglas Westerlund2, Jonas Bergquist2, Stellan Hjerten2 1Tripoli University, Tripoli, LY; 2Uppsala University, Uppsala, SE

CEPH12_WE Experimental Design Based Strategies to Optimize Capillary Electrophoresis Methods for the Separation of Polycyclic Aromatic Hydrocarbons Ludivine Ferey1, Nathalie Delaunay1, Douglas Rutledge2, Alain Huertas3, Yann Raoul4, Pierre Gareil1, Isabelle Rivals5, Jérôme Vial1 1Laboratory PECSA, Chimie ParisTech, ESPCI ParisTech, Paris, FR; 2Laboratory IAQA, AgroParisTech/INRA, Paris, FR; 3Lesieur, Asnières sur Seine, FR; 4Sofiprotéol, Paris, FR; 5Equipe de Statistique Appliquée, ESPCI ParisTech, Paris, FR

CEPH13_MO IEF-CGE as a Highly Selective Quantitative Approach for the Quantitation and Characterisation of Proteins Thomas Hahne, Hermann Wätzig Institute of Med. and Pharmaceut. Chemistry, TU Braunschweig, Braunschweig, DE

CEPH14_TU Development of a Capillary Electrophoretic Method for Determination of Plasma Clearance of Iohexol in Dogs and Cats Alexander Hellqvist1, Reidun Heiene2, Siska Croubels3, Siegrid De De Baere3, Ylva Hedeland1 1Uppsala University, Uppsala, SE; 2Norwegian School of Veterinary Science, Oslo, NO; 3Ghent University, Merelbeke, BE

CEPH15_WE Evaluation of Electro-Osmotic Markers in Aqueous and Non-Aqueous Capillary Electrophoresis Alexander Hellqvist, Ylva Hedeland, Curt Pettersson Uppsala University, Uppsala, SE

CEPH16_MO Determination of 5-Nitroimidazoles in Environmental Waters by Cation-Selective- Exhaustive-Injection and Sweeping-Micellar-Electrokinetic-Chromatography Using Dispersive-Liquid-Liquid-Microextraction for Sample Treatment Maykel Hernández-Mesa, Diego Airado-Rodríguez, Ana María García-Campaña, Carmen Cruces- Blanco University of Granada, Granada, ES

CEPH17_TU Anion Analysis in Wine by Capillary Zone Electrophoresis John C. Hudson, Jim Thorn and Hans Dewald Beckman Coulter Inc., Brea, CA, US

CEPH18_WE Label-free Electrophoretic Analysis of Carbohydrates Using Complexation with Quinolineboronic Acids. 2 Koichi Kanemori1, Takayuki Kawai2, Kenji Sueyoshi3, Takuya Kubo1, Fumihiko Kitagawa4, Koji Otsuka1 1Kyoto University, Kyoto, JP; 2National Institute of Advanced Industrial Science and Technology, Osaka, JP; 3Osaka Prefecture University, Osaka, JP; 4Hirosaki University, Hirosaki, JP

CEPH19_MO Classification of Dickeya and Pectobacterium Species by HPLC and Gel Isoeletric Focusing Anna Kubesová1, Dana Moravcová1, Jiří Šalplachta1, Jaroslav Horký2, Marie Horká1 1Institute of Analytical Chemistry of the ASCR, Brno, CZ; 2State Phytosanitary Administration, Division of Diagnostics, Olomouc, CZ

CEPH20_TU Capillary Electrophoresis Applications for the Separation and Quantification of Resin Acids Present in Rosin Samples Louise Mckeon1, Fiona Regan1, Ray Leonard2, Barry Burns2 1Dublin City University, Dublin, IE; 2Henkel, Dublin, IE

CEPH21_WE Molecularly Imprinted Monolithic Hydrophobic Polymers as a Tool for Separation in Capillary Electrochromatography Recep Uzek1, Fatma Mutlu2, Ali Derazshamshir1, Adil Denizlİ1 1Hacettepe University, Ankara, TR; 2Abant İzzet Baysal University, Bolu, TR

CEPH22_MO Separation by a Dual Mixture of Cyclodextrins Described by Overall Complexation Parameters Ludmila Müllerová, Pavel Dubský, Bohuslav Gaš Charles University in Prague, Faculty of Science, Prague, CZ

CEPH23_TU Miniaturization of Enzymatic Assays by Capillary Electrophoresis: Application to Human Kinases Implicated in Cancer and Neurodegeneration Hala Nehme, Reine Nehme, Pierre Lafite, Emilie Destandau, Sylvain Routier, Philippe Morin Institute of Organic and Analytical Chemistry, Orléans cedex 2, FR

CEPH24_WE Monitoring of Β-Galactosidase and Glycerol Kinase Activity and Inhibition Using Capillary Electrophoresis Hala Nehme, Reine Nehme, Pierre Lafite, Sylvain Routier, Philippe Morin Institute of Organic and Analytical Chemistry, Orléans cedex 2, FR

CEPH25_MO Evaluation of Capillary Gel Electrophoresis for the Development of Analytical Fingerprints for the Control of Complex Protein Mixtures of Therapeutic Interest Randa Ben Ameur, Gaëlle Coussot, Catherine Perrin Institut des Biomolécules Max Mousseron, Montpellier, FR

CEPH26_TU Sweeping–Large Volume Sample Stacking (LVSS) as Two Sequential Steps for Online Enrichment of Some Cancer Biomarkers Azza Rageh, Achim Kaltz, Ute Pyell University of Marburg, Marburg, DE

CEPH27_WE Performance of Chromatographic Systems to Estimate Biopartitioning Properties Martí Rosés, Clara Ràfols, Elisabet Fuguet, Marta Hidalgo-Rodríguez Universitat de Barcelona, Barcelona, ES

CEPH28_MO Protein-Copper Ion Mapping of Human Serum Using a Novel Metal Ion Contaminant Sweeping Technique and Fluorescent Metal Probe in Polyaminocarboxylate Gel Electrophoresis Shingo Saito1, Mitsuyoshi Kawashima1, Makoto Sato2, Masami Shibukawa1 1Saitama University, Saitama, JP; 2Sino-Test Corporation, Sagamihara, JP

CEPH29_TU Multiple Stacking Preparative Capillary Electrophoresis Using Transient Isotachophoresis Hiroto Tsujimura1, Tomoko Haraga2, Kuniaki Takahashi2, Masami Shibukawa1, Shingo Saito1 1Saitama University, Saitama, JP; 2Japan Atomic Energy Agency, Ibaraki, JP

CEPH30_WE Evaluation of CYP3A4 Enantioselective Metabolism of Verapamil by Electrophoretically Mediated Microanalysis Lucia Asensi-Bernardi1, Yolanda Martín-Biosca1, Laura Escuder-Gilabert1, Sagrario Torres- Cartas2, Salvador Sagrado3, Maria José Medina-Hernández1 1Dep. Química Analítica, Facultad de Farmacia, Univ. València, Burjassot, ES; 2IGIC-Universitat Politècnica de València, Gandia, ES; 3IDM. Unitat mixta UPV-UV, Valencia, ES

CEPH31_MO Electrophoretically Mediated Microanalysis for the Evaluation of Enantioselective Metabolism of Fluoxetine by CYP2D6 Lucia Asensi-Bernardi1, Yolanda Martín-Biosca1, Laura Escuder-Gilabert1, Sagrario Torres- Cartas2, Salvador Sagrado3, Maria José Medina-Hernández1 1Dep. Química Analítica. Facultad de Farmacia. U. València, Burjassot, ES; 2IGIC-Universitat Politècnica de València, Gandia, ES; 3IDM. Unitat mixta UPV-UV, Burjassot, ES

CEPH32_TU On-Line Capillary Electrophoresis for Studying Carboxylic Acid Production During Bioreactor Cultivation of Yeast Heidi Turkia1, Heli Sirén2, Juha-Pekka Pitkänen1 1VTT Technical research centre of Finland, Espoo, FI; 2Lappeenranta University of Technology, Lappeenranta, FI

CEPH33_WE Separation of Antibiotics Present in Biological Fluids by Capillary Isoelectric Focusing and High-Performance Liquid Chromatography with UV Detection Marie Vykydalová1, Marie Horká1, Filip Růžička2, Filip Duša1, Dana Moravcová1, Vladislav Kahle1, Karel Šlais1 1Institute of Analytical Chemistry of the ASCR, v. v. i., Brno, CZ; 2Department of Microbiology, Faculty of Medicine, Masaryk University, Brno, CZ

CEPH34_MO Fast and Validated Affinity Capillary Electrophoresis for Routine Binding Screening Assays Hermann Wätzig1, Hassan Alhazmi1, Markus Nachbar1, Deia Abd El-Hady2, Sabine Redweik1 1Univ. of Braunschweig, Braunschweig, DE; 2Assiut University, Assiut, EG

CEPH35_TU CEtoolbox: Specialized Calculator for Capillary Electrophoresis Users as an Android Application Jérome Pansanel1, Michael Biacchi2, Rabah Gahoual2, Emmanuelle Leize-Wagner2, Yannis Francois2 1IPHC, UMR 7178, Université de Strasbourg, Strasbourg, FR; 2LSMIS, UMR 7140, University of Strasbourg, Strasbourg, FR

CEPH36_WE Viral Vaccine Characterization with Capillary Electrophoresis Ewoud van Tricht1, Marta Germano1, Harold Backus1, Cari Sänger-van de Griend2 1Crucell Holland B.V., Leiden, NL; 2Kantisto B.V., Baarn, NL

Chip-Based Separations CHIP01_MO Ion-Pair Reversed-Phase Chromatography of Short Double Stranded DNA in Silicon Micro-Pillar Array Columns: Retention Model and Applications Lei Zhang1, Bivragh Majeed1, Chris Van Hoof1, Wim De Malsche2 1Imec, Leuven, BE; 2Vrije Universiteit Brussel, Brussels, BE

CHIP02_TU High Sensitive Quantitative Analysis: The Promise of Chip Based Micro LC/MS Lieve Dillen, Willy Cools, Filip Cuyckens Janssen Research and Development, Beerse, BE

CHIP03_WE Chip Electrochromatography Coupled to Mass-Spectrometric Detection by Monolithically Integrated Nanospray Emitter Claudia Ernst, Fabian Schwarzkopf, Detlev Belder University of Leipzig, Institute of Analytical Chemistry, Leipzig, DE

CHIP04_MO Considerations in Developing a Prototype Microfluidic Trapping Column to Improve Mass and Volume Capacity in an Integrated LC/MS System Jay Johnson, Jim Murphy, Angela Doneanu, Bob Jencks, Bob Collamati Waters Corporation, Milford, MA, US

CHIP05_TU Separation of Macromolecules by Miniaturized Asymmetrical Flow Field Flow Fractionation Jana Kralova1, Manly Callewaert2, Peter Schoenmakers1, Wim De Malsche2, Wim Kok1 1University of Amsterdam, Amsterdam, NL; 2Vrije Universiteit Brussel, Brussels, BE

CHIP06_WE Monitoring Enzymatic Oxidation of Polycyclic Aromatic Hydrocarbons by Microchip Electrochromatography Karin M. Krone1, Claudia Ernst1, Carlos G. Acevedo-Rocha2, Manfred T. Reetz2, Detlev Belder1 1Institute for Analytical Chemistry, University of Leipzig, Leipzig, DE; 2Max Planck Institute of Coal Research, Mülheim an der Ruhr, DE

CHIP07_MO Ultra-High Throughput Approach for Single Cell Genomics with Novel Agarose-based Microfluidic Comet Array Electrophoresis Bi-Feng Liu, Yiwei Li Huazhong University of Science and Technology, Wuhan, CN

CHIP08_TU Research-Flexible vs. Commercial Fixed-Purpose Microfluidic Chip Platforms: Can We Have the Best of Both Worlds for Flexible Field Deployable Electrodriven Analysis? Chip-ITP with Indirect Fluorescence Detection Petr Smejkal1, Michael Breadmore1, Rosanne Guijt2, Frantisek Foret3, Fritz Bek4, Mirek Macka1 1University of Tasmania, School of Chemistry, Hobart, AU; 2University of Tasmania, School of Pharmacy, Hobart, AU; 3Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, CZ; 4Agilent Technologies, Waldbronn, DE

CHIP09_WE Capillary Scale LC/MS/MS for High Sensitivity Bioanalysis Is This a Reality and Where Are the Pit Falls Paul Rainville, James Murphy, Thomas Wheat, Robert Plumb Waters Corporation, Milford, US

CHIP10_MO Liquid Chromatography in Extended Nanospace: Development of Micellar Liquid Chromatography for Dansyl Amino Acids Separation Adelina Smirnova, Kazuma Mawatari, Takehiko Kitamori The University of Tokyo, Tokyo, JP

CHIP11_TU Towards Iso-Electric Focussing of Proteins on a Chip for Potential Use in Multi- Dimensional Separations R.J. Vonk1, B.W.J. Pirok1, B. Wouters2, S. Eeltink2, W.Th. Kok1, P.J. Schoenmakers1 1Universiteit van Amsterdam, Amsterdam, NL; 2Vrije Universiteit Brussel, Brussel, BE

CHIP12_WE High Pressure Chip Chromatography with Variable On-Chip Injection and Custom- Built Particulate Columns for Void-Volume-Free Performance Sebastian Thürmann, Detlev Belder Institute of Analytical Chemistry, University of Leipzig, Leipzig, DE

CHIP13_MO Centrifugal Microfluidic Disc Integrating Carbon Monoliths for Solid-Phase Micro- Extraction Mercedes Vazquez1, Emer Duffy2, Xiaoyun He1, Elizaveta Vereshchagina1, Jens Ducrée1, Ekaterina Nesterenko1, Pavel Nesterenko2, Dermot Brabazon1, Brett Paull2 1Dublin City University, Dublin, IE; 2University of Tasmania, Hobart, AU

CHIP14_TU On-Chip Peptide Screening towards Beta-Endorphin Antibody Based on “Reverse” Affinity Chromatographic Microarray Rui Zhao, Weizhi Wang, Yanyan Huang, Yulong Jin Institute of Chemistry, Chinese Academy of Sciences, Beijing, CN

CHIP15_WE On-line Chip-based Strategy for 2D Fractionation – Comparing Peptides Found between 1D and 2D Proteomic Analysis Remco van Soest1, Xiang Zhu1, Tina Settineri1, Christie Hunter2, Jenny Albanese3 1Eksigent, part of AB SCIEX, Dublin, US; 2AB SCIEX, Foster City, US; 3AB SCIEX, Dublin, US

CHIP16_MO Design of Cyclic Olefin Copolymer-Based Microfluidic Devices Designed for Spatial Two- and Three-Dimensional Chromatography Bert Wouters1, Sander Jespers1, Gert Desmet1, Peter Schoenmakers2, Sebastiaan Eeltink1 1Vrije Universiteit Brussel, Brussel, BE; 2Universiteit van Amsterdam, Amsterdam, NL

Chiral Separations CHIR01_MO Optimization and Validation of a GC-MS Method for Determination of Free D-Amino Acids Ratio in Human Urine in a Type 1 Diabetes Study MPaz Lorenzo1, Manuel Gibellini2, Federica Pellati2, Coral Barbas1, Garcia Antonia1 1CEMBIO, Facultad de Farmacia, Universidad CEU San Pablo, Boadilla del Monte, ES; 2Department of Life Sciences, University of Modena and Reggio Emilia, Modena, IT

CHIR02_TU A Novel Approach in HPLC Chiral Method Development: Dealing with Multiple Chiral Centers David Bell1, Anders Fridstrom2, Gaurang Parmar1 1Sigma-Aldrich/Supelco, Bellefonte, PA, US; 2Sigma-Aldrich/Supelco, St Gallen, CH

CHIR03_WE A New Enantioselective HPLC Method for Analysis of Ketorolac: Application to Pharmacokinetic Study Sunil Kumar Dubey, R. N. Saha, Amit Anand Department of Pharmacy, Birla Institute of Technology & Science, Pilani, IN

CHIR04_MO Enantioselective Separation of Profens Using Immobilized Polysaccharide-Based Columns in Reverse Phase Separation Systems Radim Geryk, Denisa Plecita, Květa Kalíková, Eva Tesařová Faculty of Science, Charles University in Prague, Prague, CZ

CHIR05_TU Preparation and Characterization of 4-Isopropylcalix[4]Arene-Capped (3-(2-O-ß- Cyclodextrin)-2-Hydroxypropoxy)-Propylsilyl-Appended Silica Particles as Chiral Stationary Phase for High-Performance Liquid Chromatography S.K. ThamaraiChelvi, Jia Zhao, E.L. Yong, Yinhan Gong National University of Singapore, Singapore, SG

CHIR06_WE Pharmacokinetics Behaviour of Verapamil after Intravenous and Oral Administration of Valproic Acid in Rats by HPLC Habiba Rechak1, Mesbah Lahouel2, Abderrazak Hamdi3 1Jijel University, JIjel, DZ; 2Jijel University, Jijel, DZ; 3USTHB University, Faculty of Chemistry, Bab Ezzouar, DZ

CHIR07_MO Chiral Determination of Carvedilol and Baclofen in Plasma Samples by High- Throughput Methods Based on Ion Mobility Spectrometry (IMS)-MS-MS with HPLC Separation Adriana Iordachescu1, Luigi Silvestro2, Adelina Ciuciuleaca1, Dan Peru1, Simona Rizea-Savu2 1Pharma Serv International, Bucharest, RO; 23S-Pharmacological Consultation & Research GmbH, Harpstedt, DE

CHIR08_TU Simultaneous Quantification of Mefloquine (+)- and (-)-Enantiomers and the Carboxy Metabolite in Dried Blood Spots by LC-MS/MS Using a New Chiral Separation Approach on the Stationary Phase CHIRALPAK ZWIX(-) Mirjam Kasel1, Wolfgang Lindner2, Michael Lämmerhofer3, Reinhold Kerb1, Michael Ramharter4, Matthias Schwab1, Ute Hofmann1 1Dr.Margarete Fischer-Bosch Institute of Clin. Pharmacology, Stuttgart, DE; 2Department of Analytical Chemistry, University of Vienna, Vienna, AT; 3Institute of Pharmaceutical Science, University of Tuebingen, Tuebingen, DE; 4Division of Infectious Diseases and Tropical Medicine, Medical University Vienna, Vienna, AT

CHIR09_WE Simultaneous Two-Dimensional High-Performance Liquid Chromatographic Determination of All Proteinogenic Amino Acid Enantiomers in Human Serum Using High Performance Pirkle-Type Chiral Stationary Phases Nao Kusano1, Jumpei Sasabe2, Hirohisa Onigahara1, Yurika Miyoshi1, Masashi Mita3, Sadakazu Aiso2, Wolfgang Lindner4, Kenji Hamase1 1Kyushu University, Fukuoka, JP; 2Keio University School of Medicine, Tokyo, JP; 3Shiseido Co., Ltd, Tokyo, JP; 4University of Vienna, Vienna, AT

CHIR10_MO Approach to Enhance the Enantioseparation of Basic Drugs Using Clarithromycin as a Chiral Selector Margarita V. Lebedeva, Aleksandra F. Prokhorova, Elena N. Shapovalova, Oleg A. Shpigun Lomonosov Moscow State University, Moscow, RU

CHIR11_TU Design and Synthesis of a Novel Reagent with 6-MOQ Moiety for Amino Acid Labeling and Chiral Separations of All Proteinogenic Amino Acids Tsubasa Oyama1, Eiichi Negishi2, Nao Kusano1, Hirohisa Onigahara1, Yurika Miyoshi1, Masashi Mita3, Manabu Nakazono1, Sumio Ohtsuki4, Akio Ojida1, Wolfgang Lindner5, Kenji Hamase1 1Kyushu University, Fukuoka, JP; 2Shiseido Irica Technology Inc., Kyoto, JP; 3Shiseido Co., Ltd., Tokyo, JP; 4Kumamoto University, Kumamoto, JP; 5University of Vienna, Vienna, AT

CHIR12_WE Evaluating the Effects of Altering the Polymerization Conditions for Organic Based Monoliths on the Immobilization of Human Serum Albumin as a Binding Agent for Various Chiral Solutes in High Performance Affinity Chromatography Applications Erika Pfaunmiller1, Zhao Li1, Mahli Hartman1, Courtney Dupper1, Marie Laura Paulemond1, Shannon Lum2, Stephen Gross2, David Hage1 1University of Nebraska-Lincoln, Lincoln, NE, US; 2Creighton University, Omaha, NE, US

CHIR13_MO D- Or L-Glutamine? What Are Bacteria Using for Making Them Resistant to their Own Produced Antibiotics? A Stereochemical Identification Stefan Polnick, Orwah Saleh, Tobias Bonitz, Lutz Heide, Michael Lämmerhofer University of Tuebingen, Tuebingen, Germany, DE

CHIR14_TU Enantioselective Determination of Fluoxetine and Norfluoxetine in Wastewater by HPLC-FD Using SPE Ana R Ribeiro1, Alexandra S Maia2, Irina S Moreira3, Carlos M Afonso4, Paula ML Castro5, Maria E Tiritan6 1CICS-ISCSN-CESPU, CBQF-ESB-UCP, CEQUIMED-UP, Gandra - Paredes, PT; 2CICS-ISCSN-CESPU, CBQF-ESB-UCP, Gandra - Paredes, PT; 3CBQF-ESB-UCP, CEQUIMED-UP, Porto, PT; 4CEQUIMED- UP, Porto, PT; 5CBQF-ESB-UCP, Porto, PT; 6CICS-ISCSN-CESPU, CEQUIMED-UP, Gandra - Paredes, PT

CHIR15_WE Continuous Preparative Chromatography to Obtain Enantiomers of Verapramil Using Simulated Moving Bed (SMB) Cesar Santana1, Rafael Perna2, Marco Cremasco3 1UNIT, Aracaju- SE, BR; 2UNICAMP, Campinas - SP, BR; 3UNICAMP, Campinas-SP, BR

CHIR16_MO Continuous Chromatographic Process for Production of Secnidazole Enriched Enantiomers Ana Nascimento1, Marco Cremasco1, Cesar Santana2 1Unicamp - University of Campinas, Campinas - SP, BR; 2Unit, Aracaju, BR

CHIR17_TU Development and Validation of a LC Method for the Stability Study of a Pharmaceutical Formulation Containing Voriconazole Using Cellulose Tris(4-Chloro- 3-Methylphenylcarbamate) as Chiral Selector and Polar Non-Aqueous Mobile Phase Anne-Catherine Servais1, Radu Moldovan1, Elena Farcas1, Isabelle Roland2, Marianne Fillet1 1University of Liège, Liège, BE; 2CHU, Liège, BE

CHIR18_WE Investigation of the Retention Phenomena of Mefloquine Enantiomers Csaba Szmolnik1, Attila Felinger1, Wolfgang Lindner2 1University of Pécs, Pécs, HU; 2University of Vienna, Vienna, AT

CHIR19_MO Chiral Discrimination by High-Performance Liquid-Chromatography with Joint Use of Ion Interaction Reagents with Cyclodextrins as Additives to the Mobile Phase Kamila Szwed Institute of Physical Chemistry PAN, Warsaw, PL

CHIR20_TU Optical Isomer Separation of Flavanones and Flavanone Glycosides by Nano-Liquid Chromatography Using a Phenyl-Carbamate-Propyl-Beta-Cyclodextrin Chiral Stationary Phase Fairouz Tazerouti1, Kahina Si-Ahmed1, Ahmed Y Badjah-Hadj-Ahmed1, Zeineb Aturki2, Giovanni D’orazio2, Anna Rocco2, Salvatore Fanali2 1University of Science and Technology Houari Boumedien Usthb, Algiers, Algeria, Dz; 2a Institute of Chemical Methodologies, Italian National Council of Research, Rome, IT

CHIR21_WE Enantioselective Degradation of Enantiomers of Fluoxetine Followed by HPLC- FD Ana R Ribeiro1, Alexandra S Maia2, Irina S Moreira3, Carlos M Afonso4, Paula ML Castro5, Maria E Tiritan6 1CICS-ISCSN-CESPU, CBQF-ESB-UCP, CEQUIMED-UP, Gandra - Paredes, PT; 2CICS-ISCSN-CESPU, CBQF-ESB-UCP, Gandra - Paredes, PT; 3CBQF-ESB-UCP, CEQUIMED-UP, Porto, PT; 4CEQUIMED- UP, Porto, PT; 5CBQF-ESB-UCP, Porto, PT; 6CICS-ISCSN-CESPU, CEQUIMED-UP, Gandra - Paredes, PT

CHIR22_MO Dress-Up Chiral Columns for the Enantioseparation of Amino Acids Based on Fluorous Separation Kenichiro Todoroki1, Yasuhiro Ishii1, Koji Toyoda1, Takashi Ikawa1, JunZhe Min1, Koichi Inoue1, Shuji Akai, Toshimasa Toyo'oka University of Shizuoka, Shizuoka, JP

CHIR23_TU Electrophoretic and Molecular Docking Data Connection on the Enantioselective Binding of (±)-Catechin to Human Serum Albumin Njabulo J. Gumede1, Myalowenkosy I Sabela2, Khirsna Bisetty2, Lucia Asensi-Bernardi3, Yolanda Martín-Biosca3, Laura Escuder-Gilabert3, Maria José Medina-Hernández3, Salvador Sagrado4, Sagrario Torres-Cartas5 1Departament of Chemistry. Mangosuthu University of Technology, Jacobs, ZA; 2Departament of Chemistry. Durban University of Technology, Durban, ZA; 3Departamento Química Analítica. Facultad de Farmacia. Universitat de València, Burjassot, ES; 4Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad mixta Universitat Politècnica de València−Universitat de València, Valencia, ES; 5IGIC. Universitat Politècnica de València, Gandia, ES

CHIR24_WE Potential of Highly Sulfated Β-Cyclodextrin as Chiral Selector of Neutral and Basic Drugs in Capillary Electrophoresis Lucia Asensi-Bernardi1, Yolanda Martín-Biosca1, Laura Escuder-Gilabert1, Sagrario Torres- Cartas2, Salvador Sagrado3, Maria José Medina-Hernández1 1Departamento Química Analítica. Facultad de Farmacia. Universitat de València, Burjassot, ES; 2IGIC. Universitat Politècnica de València, Gandia, ES; 3Centro Interuniversitario de Reconocimiento Molecular y Desarrollo Tecnológico, Unidad Mixta Universitat Politècnica de Valencia-Universitat de València, Valencia, ES

Chemometrics CMTR01_MO Impact of the Nature of the Set of the Probe Solute on Orthogonality Evaluation in RPLC Ramia Al Bakain1, Isabelle Rivals2, Patrick Sassiat2, Didier Thiebaut2, Marie-Claire Hennion2, Guillaume Euvrard2, Jérôme Vial2 1The University of Jordan, Department of Chemistry, Amman, JO; 2ESPCI Paristech, Paris, FR

CMTR02_TU Characterization of Stationary Phases Based on Polysiloxanes Thermally Immobilized onto Silica and Metalized Silicas Using Supercritical Fluid Chromatography Carla Grazieli Azevedo Da Silva1, Carol Collins1, Eric Lesellier2, Caroline West2 1University of Campinas (UNICAMP), Campinas, BR; 2Université D´Orléans, Orléans, FR

CMTR03_WE Chromatographic Determination of SSRIs with Fluorescence Detection in Plasma and Urine Nitasha Agrawal1, Devasish Bose1, Neeti Prakesh Dubey2, Juan Peris-Vicente3, Samuel Carda- Broch3, Josep Esteve-Romero3 1dr. H.S.Gour University, Sagar, In; 2Regional Forensic Science Laboratory, Dharamshala, In 3University Jaume I, Castelló, ES

CMTR04_MO Determination of Distribution Coefficients of Non Steroidal Anti-Inflammatory Drugs (NSAID) by HPLC on Immobilized Artificial Membrane Columns with Current Silica Platforms Owen Foley, Dara Fitzpatrick Irish Separation Science Cluster - University College Cork, Cork, IE

CMTR05_TU General Solution of the Plate Model Including Longitudinal Diffusion and Slow Transfer Kinetics for Isocratic Chromatographic Elution María Celia García-Álvarez-Coque, Juan José Baeza-Baeza University of Valencia, Burjassot (Valencia), ES

CMTR06_WE Application of the Artificial Neural Network in Quantitative Structure Retention Relationship Modeling of Liquid Chromatography Separation of Candesartan Cilexetil and its Degradation Products Jelena Golubovic, Biljana Otasevic, Ana Protic, Mira Zecevic University of Belgrade-Faculty of Pharmacy, Belgrade, RS

CMTR07_MO Stability Studies in Different Matrices of Novel Derivatives of Allopurinol Mónica Raviolo1, Margarita Briñón1, Christian Muñoz Sosa1, Juan Peris-Vicente2, Samuel Carda- Broch2, Josep Esteve-Romero2 1Universidad Nacional Córdoba, Córdoba, AR; 2University Jaume I, Castelló, ES

CMTR08_TU Multivariate Characterisation of Chromatographic Drug-Protein Interactions Tomas Leek1, Magnus Klarqvist1, Karin Danielsson1, Johan Gottfries2 1Astrazeneca R&D Mölndal, Mölndal, SE; 2Gothenburg University, Gothenburg, SE

CMTR09_WE Probabilistic Peak Detection in One and Two Dimensional First-Order Chromatograms Martin Lopatka1, Andjoe Sampat1, Gabriel Vivo-Truyols1, Marjan Sjerps2, Arian Van Asten2, Peter Schoenmakers1 1University of Amsterdam, Amsterdam, NL; 2Netherlands Forensic Institute, the Hague, NL

CMTR10_MO Fast Determination of Saponins in Three Medicinal Plants of Panax Genus Using UPLC-DAD-CAD and Microwave-Assisted Extraction Guang-Ping Lv, De-Jun Hu, Wan-Rong Zong, Jing Zhao, Shao-Ping Li State Key Laboratory of Quality Research in Chinese Medicine, Macao, MO

CMTR11_TU A Monte Carlo Look at Multipoint Linear Calibration Veronika R. Meyer EMPA St. Gallen, St. Gallen, CH 64

CMTR12_WE Single and Combined HILIC and RPLC for the Separations of Polyphenols Casandra Ortiz Bolsico1, Sandra Pous Torres1, Gabriel Vivó Truyols2, José Ramón Torres Lapasió1, María Celia García Álvarez-Coque1, Peter J. Schoenmakers2 1University of Valencia, Burjassot, ES; 2University of Amsterdam, Amsterdam, NL

CMTR13_MO Development of a Chromatographic Method to Quantify Melamine in Swine Beatriz Beltrán-Martinavarro1, Juan Peris-Vicente1, Samuel Carda-Broch1, Josep Esteve-Romero, Sergio Marco-Peiró University Jaume I, Castelló, ES

CMTR14_TU Isocratic HPLC Optimization for Serially-Coupled Columns Considering Mobile Phase Composition, Column Nature and Length Casandra Ortiz-Bolsico, José Ramón Torres-Lapasió, María Celia García-Álvarez-Coque University of Valencia, Burjassot (Valencia), ES

CMTR15_WE Enantiomeric Composition Determination of Highly Overlapped Chromatographic Profiles by Combination of Chromatography and Chemometric Methods. Application to the Analysis of Enantiomeric Drugs Jaiver Osorio1, Cecilia Castells1, Juan Arancibia2, Alejandro Olivieri2 1Universidad Nacional De La Plata, La Plata, AR; 2Instituto De Química De Rosario, Rosario, AR

CMTR16_MO Intelligent Peak Deconvolution Analysis Through in Depth Integration of Data Matrix from Liquid Chromatography Coupled with Novel Photo-Diode Array Detector for Pharmaceutical Analysis Kanta Horie1, Takashi Kato1, Yasuhiro Mito2, Toshinobu Yanagisawa2, Masatoshi Takahashi2, Naoki Asakawa3 1Eisai Co., Ltd., Kakamigahara, JP; 2Shimadzu Corporation, Kyoto, JP; 3Eisai Co., Ltd., Tsukuba, JP

CMTR17_TU Development of Analytical Fingerprints for the Control of Complex Protein Mixtures of Therapeutic Interest Using Capillary Zone Electrophoresis Randa Ben Ameur1, Christelle Reynes2, Gaëlle Coussot1, Robert Sabatier2, Catherine Perrin1 1Institut Des Biomolécules Max Mousseron, Montpellier, FR; 2EA2415 Epidemiology Biostatistics Clinical Research, Montpellier, FR

CMTR18_WE Validation of Human Clinical Genetic Tests: The State of the Art Enrique Ochoa-Aranda1, Juan Peris-Vicente2, Samuel Carda-Broch2, Mónica-Ana Raviolo3, Maria Rambla-Alegre2, Josep Esteve-Romero2 1Hospital Provincial, Castelló, ES; 2University Jaume I, Castelló, ES; 3Universidad Nacional Córdoba, Córdoba, AR

CMTR19_MO Elution and Peak Width Triangles: a Schematic Representation of the Chromatographic Behavior in Linear Gradient Liquid Chromatography Casandra Ortiz-Bolsico, José Ramón Torres-Lapasió, Juan José Baeza-Baeza, María Celia García- Álvarez-Coque University of Valencia, Burjassot (Valencia), ES

CMTR20_TU Determination of Fungicides in Seed, Formulation, Plant Material, Soil and Water Samples Sandeep K. Mourya1, Abhilasha Durgabanshi2, Josep Esteve-Romero3, Devasish Bose1, Juan Peris-Vicente3, Samuel Carda-Broch3, Khaled Tayeb-Cherif3 1dr. H.S.Gour University, Sagar, In; 2Banaras Hindu University, Vanarasi, In; 3University Jaume I, Castelló, ES

CMTR21_WE A Universal Comparison Study of Chromatographic Response Functions used in Automated Method Development Eva Tyteca, Gert Desmet Vrije Universiteit Brussel, Elsene, BE

CMTR22_MO New Challenges in Developing Robust Criteria in Comprehensive Two-Dimensional Liquid Chromatography Methods Pascale Vanbel University of Amsterdam, Amsterdam, NL

CMTR23_TU Effectual Optimization Strategy for Ternary-Solvent Composition on Reversed Phase Liquid Chromatography Utilizing the Retention Prediction Models Tsukasa Sasaki, Takefumi Kawabe, Eiji Obara, Nobuyuki Suzuki, Toshiaki Tomitsuka Aqerl, Pt Division, Daiichi-Sankyo Co., Ltd., Hiratsuka, Kanagawa, JP

CMTR24_WE Linear Modeling of Ligand-Receptor Binding in Enantioselective Liquid Chromatography Adrian Sievers-Engler1, Wolfgang Lindner2, Michael Lämmerhofer1 1University of Tübingen, Institute for Pharmacy, Tübingen, DE; 2University of Vienna, Institute for Analytical Chemistry, Tübingen, DE

CMTR25_MO How to Choose the Appropriate Class of Stationary Phases in Relation to the Nature of the Solutes to be Analyzed Alain Tchapla1, Claire Bordes2, Didier Charbonneau3, Benjamin Chabot3, Sylvie Heron1, Jean- Luc Veuthey4, Serge Rudaz4, Pierre Lanteri2 1Univ.Paris Sud/Groupe De Chimie Analytique De Paris-Sud, Orsay, FR; 2ISA/Laboratoire Des Sciences Analytiques/ CNRS, Villeurbanne, FR; 3Interchim R & D, Montluçon, FR; 4Univ.Genève/Laboratoire De Chimie Analytique Pharmaceutique, EPGL, Genève, CH

CMTR26_TU Single Injections in Coupled Columns at Varying Solvent Content Versus Complementary Resolution Systems in the Separation of Complex Mixtures José Ramón Torres-Lapasió, Casandra Ortiz-Bolsico, María Celia García-Álvarez-Coque University of Valencia, Burjassot (Valencia), ES

CMTR27_WE SPME-LC/MSn for the Analysis of Selected Biologically Active Compounds Bogusław Buszewski, Małgorzata S Nicolaus Copernicus University, Faculty of Chemistry, Toruń, PL

CMTR28_MO Adaption of Retention Models to Allow Optimisation of Peptide and Protein Separations Patrik Petersson1, Jette Munch1, Andrey Vazhentsev2, Michael McBrien3, Karim Kassam3, Mel Euerby4 1Novo Nordisk A/S, Måløv, DK; 2Advanced Chemistry Development (ACD/Labs), Moscow, RU 3Advanced Chemistry Development (ACD/Labs), Toronto, CA; 4Strathclyde Institute of Pharmacy and Biomedical Sciences, Glasgow, GB

CMTR29_TU Chemometrics in the Development of Analytical Separation Techniques in Medicinal Plant Materials Pascale Vanbel, Joëlle Quetin-Leclercq Université catholique de Louvain, Brussels, BE

CMTR30_WE Development of an Automatic Peak Detection Algorithm for Use in Liquid Chromatography Yoachim Vanderheyden, Eva Tyteca, Gert Desmet VUB, Brussel, BE

CMTR31_MO Identification, Discrimination and Classification of Curcuma Samples Using their Chromatographic Fingerprints Johan Viaene, Goedele Alaerts, Bieke Dejaegher, Yvan Vander Heyden Vrije Universiteit Brussel, Jette, BE

Columns COLS01_MO A Comparison of HPLC Analyses of β-Blockers on C18 and C28 Reversed Phases Silica Gel Columns Imran Ali1, Norikaju Nagae2, Vinay Gaitonde3, Javed Farooqi4, Dilshad Alam1 1Jamia Millia Islamia, New Delhi, IN; 2ChromaNik Technologies Inc.,, 552-0001 Japan, Osaka, JP 3Prochrome India, Mumbai, IN; 4Indhira Gandhi National Open University, New Delhi, IN

COLS02_TU Analysis of Silicone Oils by High Performance Liquid Chromatography and Corona Charged Aerosol Detection Bruce Bailey, Ian Acworth, Marc Plante Thermo Fisher Scientific, Chelmsford, US

COLS03_WE Use of Limonene in Countercurrent Chromatography: a Green Alkane Substitute Alain Berthod, Karine Faure, Suchet Pauline, Bouju Elodie University of Lyon 1 - Institute of Analytical Science, Villeurbanne, FR

COLS04_MO Comparison of HPLC Core-Shell Columns for Isocratic Separation of Selected Xanthophylls in Egg Yolk Lučka Brulc1, Breda Simonovska1, Irena Vovk2 1National Institute of Chemistry, Ljubljana, SI; 2National Institute of Chemistry; EN-FIST Centre of Excellence, Ljubljana, SI

COLS05_TU Effect of Sample and Matrix Loading on Signal Response in LC-MS Bioanalysis Ken Butchart, Helen Lomax Fortis Technologies Ltd, Neston, GB

COLS06_WE Precision and Reliability - An Intercontinental Study of Curtain Flow Chromatography Anthony Edge1, Dominic Foley1, Michelle Camenzuli2, Luisa Pereira1, Harald Ritchie1, Andrew Shalliker2 1Thermo Fisher Scientific, Runcorn, GB; 2University of Western Sydney, Sydney, AU

COLS07_MO Comparison of Different Types of Frits in Packed Capillary Columns Martin Franc, Pavel Coufal Charles University in Prague, Faculty of Science, Prague, CZ

COLS08_TU Reverse Phase HPLC Retention Behavior of Four Poteins on Different Stationary Phases Jelena Golushko1, Oksana Rotkaja2, Edzus Picka2 1Institute of Chromatography, Riga, LV; 2University of Latvia, Riga, LV

COLS09_WE Optimization of LC Method for Simultaneous Determination of Active Compounds in Milk Thistle Dry Extract and Transfer to UPLC on Sub -2 µm and Superficially Porous Phases Natalia Halatcheva, Alexander Stoyanov, Vesela Andreeva, Stefan Avramov Sopharma JSC, Sofia, BG

COLS10_MO UPLC Performance on a Shoestring, the Adaption of an AOAC LC Method to Rapid Analysis in the Absence of UPLC Hardware Robert Hatfield, Andrew Turner Cefas, Dorset, GB

COLS11_TU The Development of a Novel Hydrolytically Stable Silica Based Stationary Phase for HPLC Matthias Ide1, Frédéric Lynen2, Pat Sandra3, Pascal Van Der Voort2 1University Ghent/Mesosol, Ghent, BE; 2University Ghent, Ghent, BE; 3University Ghent/RIC, Ghent, BE

COLS12_WE Faster and Improved HPLC Separations within Pharmacopeia Guidelines Siji Joseph1, Udo Huber2 1Agilent Technologies, Bangalore, IN; 2Agilent Technologies, Waldbronn, DE

COLS13_MO High Temperature Water Chromatography a Green Method for the Analysis of Bioactive Compounds in Birch Bark extracts Firas Jumaah, Charlotta Turner, Margareta Sandahl Lund University, Lund, SE

COLS14_TU Separation of Compounds of Biological Interest on the Diamond Analytics Flare Mixed Mode Column Supriya S. Kanyal1, Bhupinder Singh1, Michal A. Vail2, Andrew E. Dadson2, Andrew J. Miles2, Matthew R. Linford1, David Jensen2 1Brigham Young University, Provo, US; 2Diamond Analytics, Orem, US

COLS15_WE A Mix-model HPLC Stationary Phase with Reverse-phase Adsorption and Anion- exchange mechanisms Yin Lei, Yang Zhao, Wan Wang Bonna-Agela Technologies, Tianjin, CN

COLS16_MO An Affinity Protein a Column for Monoclonal Antibody (MAb) Titer Analysis Shanhua Lin, Kelly Flook, Yuanxue Hou, Hongmin Zhang, Charanjit Saini, Srinivasa Rao, Yury Agroskin, Chris Pohl Thermo Fisher Scientific, Sunnyvale, US

COLS17_TU Silica Surface Hybrid Material with Chemical Stability over a Wide pH Range for Reversed Phase Chromatography Cecilia Mazza, Fredrik Limé, Robert Fredriksson, Emelie Öhgren, Bernt Larsson Akzo Nobel PPC AB, Bohus, SE

COLS18_WE High Performance Chelation Ion Chromatography of Alkaline Earth, Transition and Lanthanide Metal Cations Using a New IDA-Silica Core-Shell Stationary Phase Nicola McGillicuddy1, Ekaterina Nesterenko1, Pavel N. Nesterenko2, Jesse O. Omamogho3, Jeremy D. Glennon3, Brett Paull4 1Irish Separation Science Cluster, Dublin City University, Dublin, IE; 2Australian Centre for Research on Separation Science, University of Tasmania, Hobart, IE; 3Irish Separation Science Cluster, University College Cork, Cork, IE; 4Australian Centre for Research on Separation Science, University of Tasmania, Hobart, AU

COLS19_MO Bed-Extrusion Based Method for the Evaluation of Packing Morphology in Analytical UHPLC Columns Tibor Müllner, Kristof Hormann, Ulrich Tallarek Philipps-Universität Marburg, Marburg, DE

COLS20_TU Development of Core Shell Particle with Large Pore for Separation of Peptides and Proteins Norikazu Nagae1, Tomoyasu Tsukamoto1, Alessandro Baldi2, Anders Grahn3, Ralf Jutvik4 1ChromaNik Technologies Inc., Osaka, JP; 2Protea Biosciences, Morgantown, US; 3Biotech AB, Onsala, SE; 4Biotech AB, Insala, SE

COLS21_WE Synthesis and Use of a Thiol Terminated Reaction Column for the Determination of Iodine Catherine Rimmer, Peter Howell National Institute of Standards and Technology, Gaithersburg, US

COLS22_MO Post Column Derivatisation Using Reaction Flow Chromatography Andrew Shalliker1, Arianne Soliven1, Maryanne Selim1, Michelle Camenzuli1, Gary Dennis1, Harald Ritchie2 1University of Western Sydney, North Parramatta, AU; 2ThermoFisher Scientific, Runcorn, GB

COLS23_TU Preparation of Crown-Ether Derivatives with Allylsilylated H8-BINOL Skeleton Acting as Chiral Stationary Phases for HPLC and their Applications to Optical Resolution of Amino Acids Shunsuke Hirata1, Keiji Nishihara2, Toyoshi Shimada1 1Nara National College of Technology, Nara, JP; 2Daicel Corporation, CPI Company, Hyogo, JP

COLS24_WE An Extensive Characterization of the Diamond-Based Flare Mixed-Mode Column Bhupinder Singh1, David S. Jensen2, Andrew E. Dadson2, Michael A. Vail2, Andrew J. Miles2, Matthew R. Linford1 1Brigham Young University, Provo, Utah, US; 2Diamond Analytics, Orem, Utah, US

COLS25_MO Tri-Peptide Based Stationary Phases for Separation of Bio-Molecules: Chromatographic Insight with Structure Function Relation Makoto Takafuji, Sudipta Ray, Hirotaka Ihara Kumamoto University, Kumamoto, JP

COLS26_TU A Qbd Approach to Eprosartan Mesylate and its Related Impurities by a Coreshell-LC Method for Ultra Fast, High Resolution, Efficient and Reproducible Analysis M V N Kumar Talluri1, Pradip Kalariya1, Vinay D. Gaitonde2 1National Institute of Pharmaceutical Education and Research, Hyderabad, IN; 2Biotech India Pvt. Ltd, Mumbai, IN

COLS27_WE On-Line Post-Column Derivatization of Amino Acids in Capillary Liquid Chromatography Jiří Vojta1, Anna Kubíčková2, Martin Franc2, Pavel Coufal2 1Charles University in Prague, Faculty of Science, Prague, CZ; 2Charles University in Prague, Faculty of Science, Department of Analytical chemistry, Prague, CZ

COLS28_MO Separation of Different Classes of Herbicides at pH 2 and pH 12 on the Diamond Analytics Flare Mixed-Mode Column Landon A. Wiest1, Bhupinder Singh1, David S. Jensen2, Andrew E. Dadson2, Michael A. Vail2, Matthew R. Linford1 1Brigham Young University, Provo, UT, US; 2Diamond Analytics, Orem, UT, US

COLS29_TU Detection and Quantification of Inorganic Arsenic in Fruit Juices by Capillary Ion Chromatography Using 4 µm Columns Hua Yang, Linda Lopez ThermoFisher Scientific, Synnyvale, CA, US

COLS30_WE Development of a High Temperature LC Method for Estrogens and Androgens for LC- IRMS Eva de Rijke, Paul Zoontjes, Hennie van Rossum, Saskia Sterk RIKILT Wageningen UR, Wageningen, NL

COLS31_MO The Utility of 2.5µm UHPLC/HPLC Columns for Laboratory Performance Emelie Öhgren, Cecilia Mazza, Luba Nilsson, Terese Tran Akzo Nobel PPC AB, Bohus, SE

COLS32_TU Evaluation of Five Core Shell Columns Based on Both Separation Behavior and Physical Property Nagae Norikazu1, Tomoyasu Tsukamoto1, Vinay Gaitonde1, Anders Grahn2, Ralf Jutvik2 1ChromaNik Technologies Inc., Osaka, JP; 2Biotech AB, Onsala, SE COLS33_WE Active Flow Management Coupled to Monolithic Columns Luisa Pereira1, Dominic Foley1, Arianne Soliven2, Anthony Edge1, Harald Ritchie1, Andrew Shalliker2 1Thermo Fisher Scientific, Runcorn, GB; 2University of Western Sydney, Sydney, AU

COLS34_MO A Novel Approach for Preparation of Porous Layer Open Tubular Capillary Columns Using Polymerisation Mixture Displacement with an Immiscible Liquid Radim Knob, Michael C. Breadmore, Rosanne M. Guijt, Jan Petr, Mirek Macka University of Tasmania, Hobart, AU

Environmental ENVI01_MO Optimization of a Solid Phase Extraction Combinated with Liquid Chromatography Tandem Mass Spectrometry Procedure to Simultaneous Determination of Pharmaceuticals Compounds in Water Samples Cristina Afonso-Olivares, Zoraida Sosa-Ferrera, Cristina Fernández-Rodríguez, José Miguel Doña- Rodríguez, José Juan Santana-Rodríguez Universidad de Las Palmas de Gran Canaria, Las Palmas de G.C., ES

ENVI02_TU Semi-Targetted Screening of Pharmaceutically-Related Contaminants in the Thames Tideway Using Liquid Chromatography-High Resolution Mass Spectrometry Edge Anthony1, Luisa Pereira1, Claudia Martins2, Kelly Munro3, Leon Barron3, David Cowan3 1Thermo Fisher Scientific, Runcorn, GB; 2Thermo Fisher Scientific, Villebon, FR; 3King's College London, London, GB

ENVI03_WE Hydrophilic Interaction Liquid Chromatography in Very Sensitive HPLC-MS/MS Methods for the Determination of Neurotoxic Domoic Acid in Lagoon Samples Elena Barbaro1, Roberta Zangrando2, Silvia Rossi2, Carlo Barbante2, Andrea Gambaro1 1University Ca' Foscari Venice, Venice, IT; 2Institute for the Dynamics of Environmental Processes CNR, Venice, IT

ENVI04_MO Comparison of the Efficiency of Three Extraction Techniques for the Determination of Quinolone Antibiotic Derivatives in Sewage Sludge Samples Noemí Dorival-García, Alberto Zafra-Gómez, Francisco Javier Camino-Sánchez, Alberto Navalón, José Luis Vílchez University of Granada, Granada, ES

ENVI05_TU Silica Supported Fe3O4 Magnetic Nanoparticles for Magnetic Solid Phase Extraction and Magnetic In-Tube Solid Phase Microextraction: Application to Organophosphorus Compounds Rodrigo González Fuenzalida, Yolanda Moliner Martínez, Carmen Molíns Legua, Helena Prima García, Antonio Ribera, Pilar Campíns Falcó, Eugenio Coronado Universitat de València, Burjassot, ES

ENVI06_WE Improved PAH Analysis with an Automated Sample Pretreatment On-Line SPE Method Vincent Goudriaan Shimadzu Benelux BV, 's-Hertogenbosch, NL

ENVI07_MO Trace Level Analysis of Herbicides in Drinking and Surface Water by Online-SPE LC/Triple Quadrupole MS to the Lower ppt Range Angelika Gratzfeld-Huesgen, Edgar Naegele Agilent Technologies, Waldbronn, DE

ENVI08_TU Ultra-High Performance Chromatography with Mass Spectrometry Detection (UHPLC-MS/MS) for the Determination of Fifteen Hormonal Compounds in Sewage from Wastewater Treatment Plants of Gran Canaria (Spain) Rayco Guedes-Alonso, Zoraida Sosa-Ferrera, José Juan Santana-Rodríguez Universidad de Las Palmas de Gran Canaria, Las Palmas de G.C., ES

ENVI09_WE Study of Photocatalytic Degradation of a Non-Ionic Surfactant (Triton X-100) by Ultrahigh-Performance Liquid Chromatography Erzsebet Szabo-Bardos, Peter Hegedus, Krisztian Horvath, Zoltan Zsilak, Otto Horvath, Peter Hajos University of Pannonia, Veszprem, HU

ENVI10_MO Determination of Endocrine Disrupting Compounds in Fish from Mediterranean Rivers Using Quechers-UPLC-MS/MS Anna Jakimska1, Belinda Huerta2, Żaneta Bargańska1, Agata Kot-Wasik1, Sara Rodríguez-Mozaz2, Damià Barceló2 1Gdańsk University of Technology, Faculty of Chemistry, Gdańsk, PL; 2Catalan Institute for Water Research, Girona, ES

ENVI11_TU UPLC-MS/MS Analysis in Support of Ecotoxicity Studies Ivonne Lammers, Erik Baltussen Theo Noij WIL Research Europe B.V., 's-Hertogenbosch, NL

ENVI12_WE Determination of Inorganic Anions in Municipal Drinking and Waste Water Using High-Pressure Modular Capillary Ion Chromatography Linda Lopez, Carl Fisher, Fei Pang, Terri Christison Thermo Fisher Scientific, Sunnyvale, US

ENVI13_MO Miniaturized Procedure for Estimating DEHP in Sediments Maria Muñoz Ortuño, Yolanda Moliner Martinez, Rosa Herraez Hernandez, Jorge Verdu Andres, Javier Pla Tolos, Pilar Campins Falco Universitat De Valencia, Burjassot (Valencia), ES

ENVI14_TU Degradation of Fluoroquinolone Antibiotics and Identification of Metabolites/Transformation Products by LC-MS/MS Alexandra S Maia1, Ana R Ribeiro2, Catarina L Amorim3, Juliana C Barreiro4, Quezia B Cass4, Paula ML Castro5, Maria Elizabeth Tiritan6 1CICS-ISCSN-CESPU, CBQF-ESB-UCP, Gandra - Paredes, PT; 2CICS-ISCSN-CESPU, CBQF- ESB-UCP, CEQUIMED-UP, Gandra - Paredes, PT; 3CBQF-ESB-UCP, CEQUIMED-UP, Porto, PT; 4DQ-UFSCar, São Carlos - SP, BR; 5CBQF/ESB-UCP, Porto, PT; 6CICS-ISCSN-CESPU, CEQUIMED-UP, Gandra - Paredes, PT

ENVI15_WE Matrix Effect and Sorption Studies of Norfloxacin on Soils Livia Maniero Peruchi, Susanne Rath University of Campinas, Campinas / São Paulo, BR

ENVI16_MO Determination of Personal Care Products and Pharmaceuticals in Reservoir Water in Colombia by Ultra-High Performance Liquid-Chromatography- Tandem Mass Spectrometry Carolina Aristizabal-Ciro, Marian Martinez-Zapata, Gustavo Peñuela-Mesa Grupo de Diagnóstico y Control de la Contaminación – GDCON, Medellin, Antioquia, CO

ENVI17_TU Screening, Quantitation and Confirmation of Pesticide Residues in Environmental Samples by UHPLC-Quadrupole Time-Of-Flight Mass Spectrometry Ana Masiá Reyes, Julian Campo, Cristina Blasco, Yolanda Picó University of Valencia, Burjassot, ES

ENVI18_WE LC-MS/MS Analysis of Pharmaceuticals in the Irish Aquatic Environment and their Potential to Bioaccumulate Up the Food Chain Gillian McEneff1, Brian Kelleher1, Leon Barron2, Brett Paull3, Brian Quinn4 1Dublin City University, Dublin, IE; 2Kings College, London, GB; 3University of Tasmania, Hobart, AU; 4Galway Mayo Institute of Technology, Galway, IE

ENVI19_MO Multi-Analyte Imprinted Polymer for the Selective Determination of Estrogens in Water Samples Gema Paniagua González, Juan Carlos Bravo, Mª Dolores Picón, Pilar Fernández National University of Distance, Madrid, ES

ENVI20_TU Amines in Ultrafine Atmospheric Aerosols: Determination by Liquid Chromatography-Triple Quadrupole Mass Spectrometry Jevgeni Parshintsev, Luis Barreira, Kari Hartonen, Marja-Liisa Riekkola University of Helsinki, University of Helsinki, FI

ENVI21_WE Ultra High Performance Separation of PAHs, Harnessing the Technological Advances of a Novel UHPLC System Wilhad Reuter, Joseph DiCesare PerkinElmer Inc., Shelton, CT, US

ENVI22_MO Two Step Pre-Concentration at Fully Microscale Column Switching Capillary Liquid Chromatography-Mass Spectrometry Allowing Versatile and Sensitive Analysis of Sulfonamides in Aqueous Environmental Samples Lucas Carvalho, Adriel Lima, Rodrigo Padovan, Fernando Lanças, Alvaro Santos-Neto Institute of Chemistry of São Carlos, University of São Paulo, São Carlos, SP, BR

ENVI23_TU Simultaneous Separation of Aliphatic and Aromatic Organic Acids by Ion- Exclusion Chromatography with Photodiode Array Detection and Atmospheric Pressure Chemical Ionization Mass Spectrometry Ludovit Schreiber, Iveta Hukelova, Radoslav Halko Faculty of Natural Sciences Comenius University in Bratisl, Bratislava, SK

ENVI24_WE Determination of Carbonyl Compounds in Biological Samples of Medical Students’ Population in the Context of Life Style and Tobacco Smoke Exposure Magdalena Szumska, Tomasz Wielkoszyński, Krystyna Tyrpień Medical University of Silesia, Katowice, PL

ENVI25_MO Development and Optimization of an Online SPE-HPLC-FD Method for Quantification of Fluoroquinolones in Wastewater Effluents Bernardo Meira1, Alexandra S Maia2, Virgínia MF Gonçalves1, Ana R Ribeiro3, Maria E Tiritan4 1CICS-ISCSN-CESPU, Gandra - Paredes, PT; 2CICS-ISCSN-CESPU, CBQF-ESB-UCP, Gandra - Paredes, PT; 3CICS-ISCSN-CESPU, CBQF-ESB-UCP, CEQUIMED-UP, Gandra - Paredes, PT; 4CICS- ISCSN-CESPU, CEQUIMED-UP, Gandra - Paredes, PT

ENVI26_TU Sorption of Enrofloxacin in Sub-Tropical Brazilian Soils Alessandra Vieira, Anne Hélène Fostier Unicamp, Campinas, BR

ENVI27_WE Simulation and Analysis of Xenon Outflow Behavior Under Multi-Pulse Injection in Dimensional Chromatography ShuJiang Liu, ZhanYing Chen, YinZhong Chang, ShiLian Wang, Qi Li, YuanQing Fan CTBT Beijing National Data Center &Radionuclide Laboratory, Beijng, CN

ENVI28_MO LC-MS/MS Method for the Determination of Parabens in Compost Samples. Comparison of the Efficiency of Two Extraction Techniques Noemí Dorival-García, Julio César Benítez-Villalba, Alberto Zafra-Gómez, Francisco Javier Camino-Sánchez, José Luis Vílchez University of Granada, Granada, ES

ENVI29_TU Study of the Influence of Temperature and Precipitations on the Levels of BETX in Natural Waters Rodrigo González Fuenzalida, Yolanda Moliner Martínez, Rosa Herráez Hernández, Jorge Verdú Andrés, Pilar Campíns Falcó, Cristina Garrido Palanca, Carmen Molíns Legua Universitat de València, Burjassot, ES

ENVI30_WE High- and Ultra-High-Performance Chromatography with Mass Spectrometry Detection Method for the Determination of Pharmaceutical Compounds in Wastewater Effluents from Wastewater Treatment Plants of Gran Canaria (Spain) Rayco Guedes-Alonso, Cristina Afonso-Olivares, Sarah Montesdeoca-Esponda, Zoraida Sosa- Ferrera, José Juan Santana-Rodríguez Universidad de Las Palmas de Gran Canaria, Las Palmas de G.C., ES

ENVI31_MO Sorption of Sulfonamides on Brazilian Soils Livia Maniero Peruchi, Keity Margareth Doretto, Susanne Rath University of Campinas, Campinas, São Paulo, BR

Food FOOD01_MO Sample Preparation with Molecularly Imprinted Polymers (MIP) SPE for Phenolic Compounds (Paraben, Guaiacol and Carsonic Acid) Sami Bayoudh, Delphine Derrien, Kaynoush Naraghi Polyintell, Val De Reuil, FR

FOOD02_TU Evaluation of Herb and Fruit Juice Adulteration and Authenticity by Coulometric Array Detection and Pattern Recognition Analysis Bruce Bailey, Qi Zhang, Marc Plante, David Thomas, Ian Acworth Thermo Fisher Scientific, Chelmsford, US

FOOD03_WE Fast, Simple, and Reproducible SPE Cleanup of Corn, Wheat, and Peanut Extracts Prior to HPLC Analysis for Mycotoxins Dave Bell1, Frank Michel2, Emily Barrey1, Ken Espenschied1, Matilal Sarker1, Michael Ye1, Olga Shimelis1 1Sigma-Aldrich/Supelco, Bellefonte, PA, US; 2Sigma-Aldrich/Supelco, Taufkirchen, DE

FOOD04_MO HPLC/MS Analysis of Phenolic Compounds in Green Tea and Correlation with their Antioxidant Capacity Lenka Ceslova, Petr Cesla, Petra Dinisova, Iva Nedopilova, Jan Fischer University of Pardubice, Department of Analytical Chemistry, Pardubice, CZ

FOOD05_TU Determination of Phytochemical Compounds in Litchi (Litchi chinensis Sonn)-flower- water-extract by HPLC and its Anti-obesity Effect Chih-Hsien Chiu1, Yi-Chen Chen1, Deng-Jye Yang2, Yi-Sheng Wu1, Yi-Ling Lin2 1National Taiwan University, Taipei, TW; 2Chung Shan Medical University, Taichung, TW

FOOD06_WE Determinations of Inorganic Anions and Organic Acids in Beverages Using Suppressed Conductivity and Charge Detection Terri Christison, Linda Lopez Thermo Fisher Scientific, Sunnyvale, CA, US

FOOD07_MO Determination of Aflatoxins in Spina Date Seed by High Performance Liquid Chromatographywith Post-Column Derivatization Xiongxiong Qiu, Hui Gao, Chao Ma, Caiyong Lin, Yin Huo, Song Zhan, Taohong Huang, Yuki Hashi Shimadzu (China) Co.,Ltd., Beijing, CN

FOOD08_TU Analysis of Black Tea by High-Resolution UPLC-MS Boudewijn Hollebrands, Christian Grün Unilever R&D Vlaardingen, Vlaardingen, NL

FOOD09_WE Antioxidant Activity and Anticancer Effect of Ethanolic and Aqueous Extracts of the Roots of Ficus Beecheyana and their Active Components Chin-Lin Hsu1, Yu-Shan Yu2, Gow-Chin Yen3, Wei-Tang Chang1 1School of Nutrition, Chung Shan Medical University, Taichung, TW; 2Health Bureau, Taichung City Government, Taichung, TW; 3Department of Food Science and Biotechnology, National Chung, Taichung, TW

FOOD10_MO Evaluation of Dispersive and Cartridge SPE Clean-up Procedures Using the Modified QuEChERS Method for Determination of Pesticide Residues in Strawberries by UHPLC-DAD Isabel Jardim, Daniele Oshita Institute of Chemistry/UNICAMP, Campinas, BR

FOOD11_TU Variability of Matrix Effects in Multi-Residue Analysis of Pesticides in Lettuce Using QuEChERS with Different Extraction Solvents and Salts Isabel Jardim, Fernanda Begnini Institute of Chemistry/ UNICAMP, Campinas, BR

FOOD12_WE Validation of a Method for Pesticide Residues Screening in Food Using UPLC-QTOF MS and Automated Data Processing Software Jean-Marc Joumier1, Sara Stead2, Dominic Roberts2, Michael McCullagh2, Ramesh Rao2, Monica Garcia Lopez3, Richard Fussell4 1Waters EHQ, St Quentin en Yvelines, FR; 2Waters Corp, Manchester, GB; 3Food and Environment research agency, York, GB; 4Food and Environment research agency, St Quentin en Yvelines, GB

FOOD13_MO Determination of Prenylflavonoid and Hop Bitter Acid in Brewer's Yeast Biomass by HPLC-DAD-MS Tsai Hua Kao, G. Y. Wu Fu Jen Catholic University, New Taipei City, TW

FOOD14_TU On-Line SPE-UHPLC-UV/VIS Method for Determination of 11 Illegal Dyes in Chili- Containing Spices Maria Khalikova, Dalibor Satinsky, Tereza Smidrkalova, Hana Sklenarova, Petr Solich Charles University in Prague, Hradec Kralove, CZ

FOOD15_WE Isolation and Identification of Major Antioxidant Components of Litchi Flower Chao-Tian Lin, Deng-Jye Yang, Yan-Zin Chang, Chuan-Hsiang Hsu Chung Shan Medical University, Taichung, TW

FOOD16_MO Analysis of B Vitamins from Feed with Liquid Chromatography Tandem Mass Spectrometry Marja Lahti1, Pertti Koivisto1, Kimmo Peltonen1 1Finnish Food Safety Authority Evira, Helsinki, FI

FOOD17_TU A Sensitive Online Enrichment System Based on Fractionized Sampling and Stacking Strategy Coupled with Ultra-High Performance Liquid Chromatography for Ultratrace Analysis of 24-Epibrassinolide in Plant Samples Jialiang Pan1, Yuling Hu1, Gongke Li1 1Department of Chemistry, Sun Yat-sen University, Guangzhou, CN

FOOD18_WE Determination of Perchlorate Levels in Food and Soil Samples Using Accelerated Solvent Extraction and Ion Chromatography Linda Lopez1, Carl Fisher1, Bruce Richter1, Brett Murphy1 1Thermo Fisher Scientific, Sunnyvale, US

FOOD19_MO Selective Online SPE via Dynamic Covalent Hydrazine Chemistry for Determination of Zearalenone in Edible Oils Silvia Marten1, Stefan Risch1, Julia Heinze2 1Wissenschaftlicher Gerätebau Dr. KNAUER GmbH, Berlin, DE; 2Beuth University of Applied Science, Berlin, DE

FOOD20_TU Carotenoid Composition of Different Kinds of Raw and Processed Pumpkins (Cucurbitaceae) Krisztina Marton, Erika Turcsi, Ferenc Kilár, József Deli University of Pécs, Pécs, HU

FOOD21_WE A Confirmatory Method for the Determination of Histamine Levels in Fishery Products by HPLC/FLD with Post-Column Derivatization Marilena Muscarella1, Sonia Lo Magro1, Carmen Palermo2, Antonio Armentano1 1IZS della Puglia e della Basilicata, Foggia, IT; 2Dipartimento di scienze Agro-Ambientali, Chimica e Difesa vegetale Università degli Studi Foggia, Foggia, IT

FOOD22_MO Highly Sensitive LC-MS/MS Analysis of Imidazole Compounds Derived from Caramel Colorants in Drinks Yasushi Nagatomi1, Rinako Hagino1, Tomonori Inoue1, Atsuo Uyama1, Naoki Mochizuki1 1Asahi Group Holdings, Ltd, Moriya, JP

FOOD23_TU Development of Method for Extraction and Chromatographic Determining of Free Sugars in Green Coffee Beans Leonel V. Constantino1, Tiago B. Madeira1, Cyntia H. G. Alves1, Bárbara S. Caldas1, Elisa Y. Hirooka1, Suzana Lucy Nixdorf1 1Universidade Estadual de Londrina, Londrina, BR

FOOD24_WE Determination of Macrolide Antibiotics in Sheep´s Milk by Molecularly Imprinted Solid Phase Extraction Coupled with High-Performance Liquid Chromatography- UV Diode-Array Detection Gema Paniagua González1, Asunción García Mayor1, Rosa María Garcinuño Martínez1, Pilar Fernández Hernando1, Jesús Senén Durand Alegría1 1National University of Distance, Madrid (Spain), ES

FOOD25_MO Determination of Silicate in Foods by High Performance Liquid Chromatography Se Jong Park1, Jae Chon Choi1, Ho Soo Lim1, Su Jin Jang1, Meehye Kim1 1Ministry of Food and Drug Safety, Chungcheongbuk-do, KR

FOOD26_TU Determination of Heterocyclic Aromatic Amines in Beefburguers by Using Microwave- Assisted Extraction (MASE) and Dispersive Liquid-Ionic Liquid Microextraction (DLILME). Optimization of the DLILME by Using a Central Composite Design Procedure Mario Reta1, Leidy Bibiana Agudelo Mesa1, Juan Manuel Padró1, Alicia Califano1 1Fac. de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, AR

FOOD27_WE Determination of Bisphenol A(BPA) in Drinking Water by HPLC-FLD Hande Ozbay1, Hande Sarman1, Deniz Sarica1, Serpil Tasdelen1, Ustun Ezer1 1Losev Food Research Lab, Ankara, TR

FOOD28_MO Authenticity and Quality Analysis of Virgin Olive Oils – Thermal Treatment Analysis Sonja Schneider Agilent Technologies, Waldbronn, DE

FOOD29_TU Identification and Quantification of 1,3-dimethylamylamine (DMAA) in Food Supplements by Liquid Chromatography Tandem Mass Spectrometry Eva Stejskalová, Radim Štěpán, Petr Cuhra, Martin Kubík Czech Agriculture and Food Inspection Authority, Prague, CZ

FOOD30_WE Investigation of the Occurrence of Vitamin E Isomers,  a Based on an Innovative Quechers Extraction Procedure Combined with Ultra Pressure Liquid Chromatography and Fluorescence Detection Babette Vanberghen1, Catarina L. Silva2, Jorge Pereira2, Karel Dekoninck1, José S. Câmara2 1Katholieke Hogeschool Kempen, Geel, BE; 2CQM, UMa, Funchal, PT

FOOD31_MO HPLC Separation of Flavanols and Methylxanthines on two C18 Core-Shell Columns Vesna Glavnik1, Breda Simonovska2, Irena Vovk1 1National Institute of Chemistry, EN-FIST Centre of Excellenc, Ljubljana, SI; 2National Institute of Chemistry, Ljubljana, SI

FOOD32_TU A New Method for the Separation of the Protein Fractions Prolamin and Glutelin in Cereal Grain by Modified SE-HPLC and SDS-PAGE Stefanie Wroblewitz1, Liane Hüther1, Hermann Wätzig2, Sven Dänicke1 1Fredrich-Loeffler-Institute, Institute of Animal Nutrition, Braunschweig, DE; 2Technische Universität Braunschweig, Institute of Medicinal and Pharmaceutical Chemistry, Braunschweig, DE

FOOD33_WE Determination of Quality Indicators and Antioxidant Content in Various Fruit Juices Blanka Švecová1, Kateřina Andrlová University of Pardubice, Pardubice, CZ

FOOD34_MO Development of a Dispersive Liquid–Ionic Liquid Microextraction Method for the Determination of Nifurtimox and Benznidazole in Human Breast Milk Mario Reta1, Juan Manual Padró1, Rocío Pellegrino Vidal1, Leidy Bibiana Agudelo Mesa1, Alicia Califano, María Elena Marsón, Guido Mastrantonio Fac. de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, AR

FOOD35_TU The Use of Microflow UHPLC in Mycotoxin Analysis Stephen Lock AB SCIEX, Pudsey, GB

Forensic FORC01_MO Multiclass Detection and Quantification of Drugs of Abuse in Biological Samples Based on the Combination UHPLC-MS/MS with Microextraction Techniques Diego Airado-Rodríguez, Manuel Lombardo-Agüí, Carmen Cruces-Blanco, Ana María García- Campaña University of Granada, Granada, ES

FORC02_TU Determination of the Nitrogen Content of Nitrocellulose by Capillary Electrophoresis after Alkaline Denitration Elodie Alinat1, Nathalie Delaunay2, Christine Costanza3, Xavier Archer3, Pierre Gareil2 1PECSA/Central Laboratory of the Préfecture De Police of Pari, Paris (France), FR; 2Laboratory of Physicochemistry of Electrolytes, Colloids, and Analytical Sciences (PECSA), Paris (France), FR; 3Central Laboratory of the Préfecture De Police of Paris, Paris (France), FR

FORC03_WE Microwave-Assisted Extraction Followed by the Chromatographic Determination of Antidepressants in Plasma Antonia M Carro, Rosa A. Lorenzo, María Regenjo, Ana Bermejo, Purificación Fernández University of Santiago De Compostela, Santiago De Compostela, ES

FORC04_MO Capillary Electrophoresis in Combination with Laser Induced Fluorescence Detection for the Sensitive Analysis of LSD and ISO-LSD in Human Hair Samples Diego Airado-Rodríguez, Maykel Hernández-Mesa, Carmen Cruces-Blanco, Ana María García- Campaña University of Granada, Granada, ES

FORC05_TU Highly Sensitive Capillary Electrophoresis-Mass Spectrometry for Rapid Screening and Accurate Quantitation of Drugs of Abuse in Urine Isabelle Kohler1, Julie Schappler1, Martin Greiner2, Serge Rudaz1 University of Geneva, Geneva 4, CH; 2Agilent Technologies, Waldbronn, DE

FORC06_WE Determination of Nimetazepam and 7-Amino Nimetazepam in Human Urine Using LC- MS/MS Kuang-Chuan Wang1, Jen-Der Wu2, Ching-Kuo Lee2 1Forensic Science Division, Criminal Investigation Bureau, Taipei, TW; 2School of Pharmacy,Taipei Medical University, Taipei, TW

FORC07_MO UPLC-PDA and Dispersive Liquid-Liquid Microextraction for Determination of Drugs of Abuse in Human Plasma Rosa A Lorenzo1, Antonia M Carro1, María Regenjo1, Ana M. Fernández2, Purificación Fernández1 1University of Santiago De Compostela, Santiago De Compostela, ES; 22Drug Addiction Health Care Unit, Monforte De Lemos, ES

FORC08_TU Exploring Low and High pH Eluents Using a New Silica-Based Extended pH Range C18 Column for Analysis of Amphetamines & Opiates from Urine by LC-MS Alan Mckeown1, Carl Zimmerman2 1Advanced Chromatography Technologies Ltd, Aberdeen, Scotland, GB; 2macmod Analytical Inc., Chadds Ford, Pa 19317, US

FORC09_WE LC-MS/MS Method for the Determination of 21 Opiates and Opiate Derivatives in Urine Luisa Pereira, Joanne Jones, Steve Westwood, Tim Liddicoat, Tony Edge Thermo Fisher Scientific, Runcorn, GB

FORC11_TU Confirmation of the Presence of Gunshot Residue by Means of Organic Compounds Using Time of Flight Mass Spectrometry Alicia Sanchez, Sandra Benito, Zuriñe Abrego, Nora Unceta, Maria Aranzazu Goicolea, Ramon J. Barrio University of the Basque Country, Vitoria-Gasteiz, ES

FORC12_WE Novel UPLC Columns for the Analysis of Synthetic Cannabinoids and Metabolites in Whole Blood for Forensic Toxicology Mia Summers, Jonathan Danaceau, Erin Chambers, Kenneth Fountain Waters, Milford, Ma, US

FORC13_MO Cation-Selective Exhaustive Injection and Sweeping MEKC for Analysis of Methadone and its Metabolites in Serum of Heroin Addicts Shou-Mei Wu Kaohsiung Medical University, Kaohsiung, TW

Fundamentals FUND01_MO Predicting Chromatographic Gradient Elution of Acid-Base Compounds with Simplified Models Axel Andrés, Martí Rosés, Elisabeth Bosch Departament de Química Analítica, Universitat de Barcelona, Barcelona, ES

FUND02_TU Influence of Wide Pore Size Distribution in Size-Exclusion Chromatography Ivett Bacskay, Annamária Sepsey, Attila Felinger University of Pécs, Pécs, HU

FUND03_WE Microstructural Characterization of Random Hard-Sphere Packings Using Pore-Size Entropy Vasili Baranau, Dzmitry Hlushkou, Siarhei Khirevich, Ulrich Tallarek Philipps-Universität Marburg, Marburg, DE

FUND04_MO Monodisperse Particles in HPLC Columns David Bell1, Richard Henry1, William Cambell1, William Betz1, Carmen Santasania1, Anders Fridstrom2, Frank Michel1, Gaurang Parmar1, Roy Eksteen1 1Sigma-Aldrich/Supelco, Bellefonte, PA, US; 2Sigma-Aldrich/Supelco, St. Gallen, CH

FUND05_TU The Scientist Is No Longer in the Laboratory, but Integrated in the Overall Quality Process Peter Boogaard Industrial Lab Automation, Moordrecht, NL

FUND06_WE Multiresidue Determination of Pesticides in High Water Content Vegetable and Fruits by LC-MS/MS and GC-MS/MS Hande Ozbay, Hande Sarman, Deniz Sarica, Serpil Tasdelen, Ustun Ezer LOSEV Food Research Lab, Ankara, TR

FUND07_MO Transfer Prediction from HPLC to UHPLC by Using Modelling Software Frédéric Goldschmidt1, Barbara Channer1, Imre Molnár2 1Actelion Pharmaceuticals Ltd., Allschwil, CH; 2Molnár-Institute, Berlin, DE

FUND08_TU Validated Gradient Stability Indicating HPLC Method for Determining Diltiazem Hydrochloride and Related Substances in Bulk Drug and Novel Tablet Formulation Vivekanand Chatpalliwar, Pawan Porwal, Santosh Chajjed SNJB's SSDJ College of Pharmacy, Chandwad, IN

FUND09_WE Determination and Comparison of Geniposide Content Following Oral Administration of Geniposide, Gardenia Jasminoides Ellis, and Gardenia Herbal Formulation in Rats by High Performance Liquid Chromatography Sharleen Cheng1, Lie-Chwen Lin2, Chi-Hung Lin1, Tung-Hu Tsai1 1National Yang-Ming University, Taipei, TW; 2National Research Institute of Chinese Medicine, Taipei, TW

FUND10_MO Computer Organized Screening and Method Optimization System – Phase 2 (COSMOS 2) Gabriela Cimpan1, Rudy Sneyers2, Jeroen Peeters2, Vivienne Malanchin2, Andrey Vazhentsev3 1Advanced Chemistry Development (ACD/Labs), Bracknell, GB; 2Janssen Pharmaceutical Companies of J&J, Beerse, BE; 3Advanced Chemistry Development (ACD/Labs), Moscow, RU

FUND11_TU A Fundamental Study of Peak Enhancement in Liquid Chromatography Jelle De Vos, Gert Desmet, Sebastiaan Eeltink Vrije Universiteit Brussel, Brussels, BE

FUND12_WE An Operation Platform for the Automation of Biopolymer Separation by Liquid Chromatography Xindu Geng1, Fei Wang1, Peng Liu1, Huiqiang Zhang2 1Northwest University, Xi'an, CN; 2Northwest, Xi"an, CN 81

FUND13_MO Analysis of the Random-Close Packing Limit of Polydisperse Hard-Sphere Packings Vasili Baranau, Ulrich Tallarek Philipps-Universität Marburg, Marburg, DE

FUND14_TU A Mathematical Relationship between the Freundlich and the Bilangmuir Isotherms George Horvai Budapest University of Technology and Economics, Hungary, HU

FUND15_WE Determination of Bioactive Components in Chinese Herbal Formulae and Pharmacokinetics of Rhein in Normal and Constipated Rats by UPLC-MS/MS and Gene Expression Profiling in Drug Metabolizing Genes Mei-Ling Hou1, Li-Wen Chang1, Lie-Chwen Lin2, Chi-Hung Lin3, Tung-Hu Tsai1 1Institute of Traditional Medicine, NYMU, Taipei, TW; 2National Research Institute of Chinese Medicine, Taipei, TW; 3Institute of Microbiology and Immunology, NYMU, Taipei, TW

FUND16_MO Fast Reversed Phase HPLC Method for Content Determination of Insulin Analogues Aage Hvass1, Karin Juul Jensen1, Gillian Danielsen1, Jette Christensen1, Marianne Nielsen2, Rikke Pedersen2 1Novo Nordisk A/S, Måløv, DK; 2Novo Nordisk A/S, Bagsværd, DK

FUND17_TU Improving Productivity in Natural Product Compound Isolation Using Isocratic Preparative Liquid Chromatography Jo-Ann Jablonski, Rui Chen Waters Corporation, Milford, MA, US

FUND18_WE High Temperature Liquid Chromatography–Aerosol Detector Analysis: Effect of Elution Bandwidth on Detection Response Manish Khandagale, Joseph Hutchinson, Gregory Dicinoski, Paul Haddad University of Tasmania - ACROSS, Hobart (Tasmania), AU

FUND19_MO Screening of Multiple Oxidative Hair Dyes by High Performance Liquid Chromatography Jia Yi Kwang, Kin Har Kiang, Nuan Ping Cheah Health Sciences Authority, Singapore, SG

FUND20_TU Analysis of Transverse Dispersion in Bulk Structures: Sphere Packings, Regular Pillar Arrays, and Silica Monolith. Anton Daneyko, Dzmitry Hlushkou, Siarhei Khirevich, Ulrich Tallarek Philipps Universitaet Marburg, Marburg, DE

FUND21_WE Determination of Diuretics in Urine Using Multi-Walled Carbon Nanotubes as Sorbent in Dispersive Solid Phase Extraction Combined with Liquid Chromatography Tandem Mass Spectrometry Maw-Rong Lee, Tse-Tsung Ho National Chung Hsing University, Taichung, TW

FUND22_MO Spider Diagram: A Universal and Versatile Approach for Chromatographic System Comparison and Classification. Part 1: Solvent Classification Eric Lesellier, Caroline West ICOA, Université Orléans, Orléans, FR

FUND23_TU Determination of Molar Mass and Size Distributions of Polymeric and Colloidal Samples by Taylor Dispersion Analysis Hervé Cottet1, Jean-Philippe Biron1, Luca Cipelletti1, Michel Martin2 1University of Montpellier, Montpellier, FR; 2ESPCI, Paris, FR

FUND24_WE Retention Prediction of Ionizable Analytes in RP-HPLC by Organic Modifier Gradients in Different Eluent PH’s Chrysostomi Zisi, Stella Fasoula, Ahmed Mansour, Panagiotis Nikitas, Adriani Pappa-Louisi Aristotle University of Thessaloniki, Thessaloniki, GR 82

FUND25_MO Comparison between Refractometry, Spectrophotometry and Liquid Chromatography in Determination of Total Free Sugars in Commercial Juices and Nectars of Grape and Orange Leonel Constantino, Cyntia Alves, Bárbara Caldas, Elisa Hirooka, Suzana Nixdorf Universidade Estadual de Londrina, Londrina, BR

FUND26_TU Validated Stability Indicating HPLC Method for Determining Acitretin in Bulk Drug and Pharmaceutical Preparation Pawan Porwal, Vivekanand Chatpalliwar, Jayesh Patil SNJB's SSDJ College of Pharmacy, Nasik (Maharashtra), IN

FUND27_WE High Through-Put and High Sensitivity Analyses Using Active Flow Technology Chromatography with Mass Spectral Detection. Andrew Shalliker1, Danijela Kocic1, Luisa Pereira2, Tony Edge2, Jackie Mosely3, Harald Ritchie2, Dominique Foley2, Xavier Conlan4 1University of Western Sydney, North Parramatta, AU; 2ThermoFisher Scientific, Runcorn, GB; 3Durham University, Durham, GB; 4Deakin University, Geelong, AU

FUND28_MO On-Line Determination of Arsenic Species in Rice by High Performance Liquid Chromatography – Inductively Coupled Plasma Spectrometry (HPLC-ICP-MS) Pinar Nas, Hande Sarman, Deniz Sarica, Serpil Tasdelen, Ustun Ezer LOSEV Food Research Lab, Ankara, TR

FUND29_TU Optimization of Concentration Gradient Analyses for Highly Reproducible Results in VHPLC Joseph Stankovich, Fabrice Gritti, Paul Stevenson, Georges Guiochon University of Tennessee, Knoxville, US

FUND30_WE SAXS (Small Angle X-Ray Scattering) Investigation of Mesoporous Chromatographic Adsorbents and Correlation with Physisorption Experiments Daniela Stoeckel1, Dirk Wallacher2, Gerald Zickler3, Ulrich Tallarek4, Bernd Smarsly1 1Justus-Liebig-University, Giessen, DE; 2Helmholtz Center, Berlin, DE; 3Montan-University, Leoben, AT; 4Philipps-University, Marburg, DE

FUND31_MO A Rapid HPLC/PDA Method for the Evaluation of Labeling Accuracy of Soy Dietary Supplements Commercially Available in Romania Alina Uifalean, Cristina Iuga University of Medicine and Pharmacy, Cluj-Napoca, RO

FUND32_TU Imaging of Non-Uniform Motion of Single DNA Molecules Reveals the Kinetics of Varying-Field Isotachophoresis Hailin Wang, Shengquan Liu, Bailin Zhao, Dapeng Zhang, Cuiping Li Research Center for Eco-Environmental Sciences, Beijing, CN

FUND33_WE Spider Diagram: A Universal and Versatile Approach for Chromatographic System Comparison and Classification. Part 2: Stationary Phases Eric Lesellier, Caroline West ICOA, Université Orléans, Orléans, FR

FUND34_MO UHPLC Getting it Right! Considerations for Optimal Performance! Mark Woodruff, Ken Butchart Fortis Technologies Ltd, Neston, GB

FUND35_TU Screening and Evaluation of Adulterants in 247 Illegal Sexual Performance Enhancement Products Using HPLC-DAD and GC-MS Yimin Xu, Min Yong Low, Yun Zeng Health Sciences Authority of Singapore, Singapore, SG

FUND36_WE Multilinear Gradient Elution Optimization in Combined pH/Organic Solvent Gradient RP-HPLC Chrysostomi Zisi, Stella Fasoula, Ahmed Mansour, Panagiotis Nikitas, Adriani Pappa-Louisi Aristotle University of Thessaloniki, Thessaloniki, GR

FUND37_MO Novel Methods for Detection and Quantitation of Impurities Using a New High Sensitivity Photodiode Array Detector Daisuke Nakayama, Yusuke Osaka, Masatoshi Takahashi, Toshinobu Yanagisawa, Junichi Masuda, Okiyuki Kunihiro Shimadzu Corporation, Kyoto, JP

Glycans GLYC01_MO Characterization of Glycans by Capillary-Scale Anion Exchange Chromatography Hyphenated to Ion Trap Mass Spectrometry Cees Bruggink1, Franck van Veen2, Remco Swart3, André M. Deelder1, Manfred Wuhrer1 1LUMC, Leiden, NL; 2Thermo Fisher Scientific, Breda, NL; 3Thermo Fisher Scientific, Amsterdam, NL

GLYC03_WE Comparison of Released N-Glycans between the Innovator and a Candidate Biosimilar: A Case Study on Etanercept. Stephane Houel, Ying Qing Yu, Eoin Cosgrave, Weibin Chen Waters Corp., Milford, US

GLYC04_MO Oriented Immobilization of PNGase F on a Monolithic Support for Glycoprotein Analysis Jana Krenkova1, Akos Szekrenyes2, Zsolt Keresztessy3, Frantisek Foret1, Andras Guttman2 1Institute of Analytical Chemistry, v.v.i., Brno, CZ; 2Horvath Laboratory of Bioseparation Sciences, University of Debrecen, Debrecen, HU; 3Debrecen Clinical Genomics Center, University of Debrecen, Debrecen, HU

GLYC05_TU Development of New Bioaffinity Phases for Glycoprotein Separation and Analysis Ruth Larragy1, Brendan O’Connor2, Michael O’Connell3, Paul Clarke4, Roisin Thompson4, Damien Keogh3, Amy Harrington2, Norah Cassidy2, Colm Cooke2, Triona O’Connell1 1Irish Separation Science Cluster, Dublin, IE; 2Irish Separation Science Cluster, School of Biotechnology, Dublin, IE; 3Irish Separation Science Cluster, School of Biotechnology, Dublin, IE 4ProLegere, Dublin, IE

GLYC06_WE Site-Specific N-Glycosylation Analysis of Human Immunoglobulin E Rosina Plomp, Gerhild Zauner, Paul Hensbergen, André Deelder, Manfred Wuhrer Leiden University Medical Centre, Leiden, NL

GLYC07_MO Capillary Electrophoresis with Contactless Conductivity Detector for Myrosinase Activity Assays Reine Nehme, Gregory Roux, Hala Nehme, Patrick Rollin, Arnaud Tatibouet, Philippe Morin Institute of Organic and Analytical Chemistry, Orléans cedex 2, FR

GLYC08_TU Separation of Labeled and Native N-Glycans on a Novel Mixed-Mode Stationary Phase Xiaodong Liu, Udayanath Aich, Christopher Pohl Thermo Fisher Scientific, Sunnyvale, California, US

GLYC09_WE Unambiguous Characterization of Glycosylation Patterns of Monoclonal Antibodies - Integrated Use of LC-MS/MS and NMR Techniques Alena Wiegandt, Bernd Meyer Insitute of Organic Chemistry - University of Hamburg, Hamburg, DE

GLYC10_MO Workflow for High-Throughput Negative Ion Mode MALDI-TOF-MS Analysis of Complex N-Glycan Samples Karli Reiding, Dennis Blank, André Deelder, Manfred Wuhrer Leiden University Medical Center, Leiden, NL

HILIC HILC01_MO Analysis of Highly Hydrophilic Compounds by Using Inorganic/Organic Hybrid Based Reversed-Phase and HILIC Columns Friederike Becker1, Noriko Shoji2, Takashi Sato2, Takatomo Takai2, Naohiro Kuriyama2 1YMC Europe GmbH, Dinslaken, DE; 2YMC Co., Ltd., Komatsu, JP

HILC02_TU Fundamental Investigation Regarding Robustness and Repeatability in HILIC Chromatography David Bell, Craig Aurand, Hugh Cramer, Gaurang Parmar Sigma-Aldrich/Supelco, Belleofnte, PA, US

HILC03_WE A Comparative Study of Two HPLC Columns in the Determination of Phenoxy Acid Pesticides Sagrario Torres-Cartas, Susana Meseguer-Lloret, Carmen Gómez-Benito, Mónica Catalá-Icardo IGIC-Universitat Politènica de València, Gandia (València), ES

HILC04_MO Determination of Carbamate Pesticides by HPLC-Photoinduced Chemiluminescence Mónica Catalá-Icardo, Susana Meseguer-Lloret, Sagrario Torres-Cartas, Carmen Gómez-Benito IGIC-Universidad Politécnica de Valencia, Gandía-Valencia, ES

HILC05_TU Polyanionic Monosized Particles as a Stationary Phase for Hydrophilic Interaction Chromatography Gunes Kibar, Bekir Celebi, Ali Tuncel Hacettepe Uni, Ankara, TR

HILC06_WE Determination of Paraquat and Diquat in Environmental Samples Using a New High Efficiency Ultraperformance LC® Column for HILIC Kenneth Fountain, Jeremy Shia, Michael Young, Mia Summers Waters Corporation, Milford, MA, US

HILC07_MO Determination of Tartaric Acid in Human Plasma by Hydrophilic Interaction Liquid Chromatography Coupled to Mass Spectrometry Evgueni Fedorov, Michel Coutu, Jean-François Larocque, Michael Mancini Biotrial Bioanalytical Services, Laval, QC, CA

HILC08_TU Development of a Quantitative LC-MS/MS Assay for the Simultaneous Quantitation of Acetylcholine, Histamine, and their Metabolites in Human Cerebrospinal Fluid (CSF) Using Sub 2µm HILIC UPLC Kenneth Fountain, Mary Lame, Erin Chambers Waters, Milford, US

HILC09_WE Do Electronic Cigarettes Help in Quitting Nicotine and Smoking Addiction? Evaluation of Nicotine Content in Zero-Level E-Liquids with the Approach “Dilute and Shoot” by RPLC-MS/MS and HILIC-MS/MS Paweł Kubica, Kamila Wilczewska, Andrzej Wasik, Agata Kot-Wasik, Jacek Namieśnik Gdansk University of Technology, Gdansk, PL

HILC10_MO Comparative Study of Retention Behavior for Water-Soluble Vitamins on Diol-, Triazole- and Sulfobetaine- Modified Silica Columns in HILIC Mode Alexey Lapin Euroscreen SA, Gosselies, BE

HILC11_TU Using Chromatographic Information to Validate Molecular Docking Estimations. A Case Study Njabulo J. Gumede1, Parvesh Singh2, Myalowenkosy I Sabela2, Khirsna Bisetty2, Yolanda Martin- Biosca3, Laura Escuder-Gilabert3, Maria José Medina-Hernández3, Salvador Sagrado4, Sagrario Torres-Cartas5 1Departament of Chemistry. Mangosuthu University of Technology, Jacobs, ZA; 2Departament of Chemistry. Durban University of Technology, Durban, ZA; 3Departamento de Química Analítica, Facultad de Farmacia, Universitat de València, Burjassot, ES; 4Centro de Reconocimiento Molecular y Desarrollo Tecnoló gico (IDM), Unidad mixta Universitat Politècnica de València−Universitad de València), Valencia, ES; 5IGIC-Universitat Politècnica de València, Gandia, ES

HILC12_WE Separation of Polar Compounds in Hydrophilic Interaction Chromatography Mode Using a New, Polymer Based Zwitterionic Stationary Phase Gunes Kibar, Ali Tuncel Hacettepe Uni, Ankara, TR

HILC13_MO Cyclofructan and Amide Based Stationary Phases for Hydrophilic Interaction Liquid Chromatography Kveta Kalikova1, Petr Kozlik1, Martin Gilar2, Daniel Armstrong3, Eva Tesarova1 1Charles Universtiy in Prague, Prague, CZ;2Waters Corporation, Milford, US; 3The University of Texas at Arlington, Arlington, US

HILC14_TU Evaluation of Different Column Chemistries for Fast Urinary Metabolic Profiling Dick-Paul Kloos1, Henk Lingeman1, Wilfried Niessen1, André Deelder2, Oleg Mayboroda2, Martin Giera2 1Free University Amsterdam, Amsterdam, NL; 2Leiden University Medical Center, Leiden, NL

HILC15_WE Method Development Approaches in HILIC Anthony Edge, Monica Dolci, Luisa Pereira Thermo Fisher Scientific, Runcorn, GB

HILC16_MO Polar Basic Drugs in Environmental Samples; Improved Analysis Using a New High Efficiency Ultraperformance LC® Column for HILIC Kenneth Fountain, Michael Young, Mia Summers Waters Corporation, Milford, MA, US

HILC17_TU Acetone Based HILIC SPE As an Alternative Approach for Eliminating Matrix Effects in LC-MS. Thomas Van Damme1, Miroslava Lachová1, Frederic Lynen1, Roman Szucs2, Pat Sandra1 1University of Ghent, Ghent, BE; 2Pfizer Global R&D, Sandwich, GB

HILC18_WE HPLC-Chemiluminesce for the Determination of Organothiophosphorus Pesticides Mónica Catalá-Icardo1, Luis Lahuerta-Zamora2, Sagrario Torres-Cartas1, Susana Meseguer-Lloret1 1IGIC-Universidad Politécnica de Valencia, Gandía-Valencia, ES; 2Universidad CEU-Cardenal Herrera, Moncada-Valencia, ES

HILC19_MO Evaluation of Amide and C28 Phases for Separation of Hydrophilic Compounds Tsukamoto Tomoyasu1, Norikazu Nagae1, Vinay Gaitonde1, Anders Grahn2, Ralf Jutvik2 1ChromaNik Technologies Inc., Osaka, JP; 2Biotech AB, Onsala, SE

HILC20_TU Sample Preparation and Analysis of Pteridines in Urine by Means of UHPLC-FD with Emphasis on their Stability Helena Tomšíková, Lucie Nováková Faculty of Pharmacy in HK, Charles University in Prague, Hradec Králové, CZ

HILC21_WE Systematic Comparison of Sensitivity between HILIC-MS and RPLC-MS for Analysis of Pharmaceutical Compounds Aurélie Périat, Jean-Luc Veuthey, Serge Rudaz, Davy Guillarme University of Geneva, Geneva, CH

HILC22_MO A New HPLC-Chemiluminescence Method for the Analysis of MCPB in Water Samples Sagrario Torres-Cartas, Susana Meseguer-Lloret, Mónica Catalá-Icardo, Carmen Gómez-Benito IGIC-Universitat Politènica de València, Gandia (València), ES

HILC23_TU Fit-For-Purpose HPLC Method for Determining the Antibiotic Amikacin in Human Plasma from Patients Using O-Phthalaldehyde - N-Acetyl-L-Cysteine (OPA-NAC) as a Fluorogenic Pre-Column Reagent Carlos Ezquer-Garin1, Yolanda Martín-Biosca1, Laura Escuder-Gilabert1, María José Medina- Hernández1, Salvador Sagrado2, Mónica Catalá-Icardo3 1Departamento de Química Analítica. Facultad de Farmacia. Universitat de València., Burjassot, ES; 2Centro Interuniversitario de Reconocimiento Molecular y Desarrollo Tecnológico. Unidad Mixta Universidad Politécnica de Valencia-Universitat de València., Burjassot, ES; 3IGIC- Universidad Politécnica de Valencia, Gandía, ES

High-Resolution MS HRMS01_MO Determination of Pristine Fullerenes and Surface Functionalized Fullerenes in Environmental Samples by UHPLC-APPI-MS(/MS) Alina Astefanei, Oscar Núñez, Encarnacion Moyano, Maria Teresa Galceran University of Barcelona, Barcelona, ES

HRMS02_TU Photodegradation of Tetracycline Antibiotics in Natural Water Samples Anna Jakimska, Kamila Wilczewska, Weronika Hewelt-Belka, Agata Kot-Wasik, Andrzej Wasik, Jacek Namieśnik Gdańsk University of Technology, Faculty of Chemistry, Gdańsk, PL

HRMS03_WE A Novel High Resolution Fractionation Technique Combined with High Resolution Mass Spectrometry to Identify Toxic Compounds in Estuarine and Marine Waters Petra Booij1, Pim Leonards1, Sascha Sjollema2, Pim de Voogt2, Dick Vethaak1, Jeroen Kool3, Marja Lamoree1 1Institute for Environmental Studies, Amsterdam, NL; 2University of Amsterdam, Amsterdam, NL; 3Vrije Universiteit Amsterdam, Amsterdam, NL

HRMS04_MO Gradient Transfer for a Rapid and Efficient Large Scale Isolation of Natural Products on MPLC-UV-ELSD Platform with Enhanced Pressure and Temperature Control Soura Challal, Emerson Ferreira Queiroz, Werner Kloeti, Davy Guillarme, Jean-Luc Wolfender University of Geneva, Geneva 4 (Switzerland), CH

HRMS05_TU A Highly Sensitive Method for the Quantification of Key Oxysterols in Plasma Using Liquid Chromatography with High Resolution Mass Spectrometry Stefania Della Gatta, Daniel Arndt, Mark Bentley Philip Morris International R&D, Neuchâtel, CH

HRMS06_WE Development of Qualitative Method for Marker Compounds of Bang-Poong-Tong- Sung-San by Iontrap-Time of Flight (IT-TOF) Mass Spectrometry Han Young Eom, Unyong Kim, Joon Hyuk Suh, Junghyun Kim, Hyun-Deok Cho, Juhee Jung, Yura Jung, Jeong-Rok Youm, Sang Beom Han Chung-Ang University, Seoul, KR

HRMS07_MO Environmentally Friendly Method to Determine Polar Benzotriazoles, Benzothiazoles and Benzenesulfonamides Derivates in Sewage Sludge by Pressurised Hot Water Extraction and Liquid Chromatography-High Resolution Mass Spectrometry Pol Herrero, Francesc Borrull, Eva Pocurull, Rosa Maria Marcé Universitat Rovira i Virgili, Tarragona, ES

HRMS08_TU Qualitative and Semi-Quantitative Analysis of Intact Chicken Ovalbumin by High- Resolution Native Mass Spectrometry Reveals 59 Proteoforms Arjan Barendregt, Yang Yang, Albert J.R. Heck Utrecht University, Utrecht, NL

HRMS09_WE Elucidation of Degradation Pathway of Ketoprofen and Ibuprofen by UPLC-QTOF-MS Anna Jakimska, Paweł Kubica, Agata Kot-Wasik, Andrzej Wasik, Jacek Namieśnik Gdańsk University of Technology, Faculty of Chemistry, Gdańsk, PL

HRMS10_MO Characterization of Selected Drug Metabolites in Rat Tissues Using Combined Information from UHPLC/ESI-MS/MS and MALDI-MS imaging Robert Jirásko1, Michal Holčapek1, Ivan Vokřál2, Vitaliy Chagovets1 1University of Pardubice, Pardubice, CZ; 2Faculty of Pharmacy, Charles University, Hradec Králové, CZ

HRMS11_TU LC-LC-MS/MS Heart-cut Approach for Software assisted Pharmaceutical Impurity Identification from a non-ms compatible LC method Siji Joseph1, Yuqin Dai2 1Agilent Technologies, Bangalore, IN; 2Agilent Technologies, Santa Clara, US

HRMS12_WE Separation, Identification and Characterization of Stress Degradants and Related Impurities of Bortezomib Using LC and LC-MS/MS M V N Kumar Talluri, Divya Chundi, Srinivas Ragampeta National Instutute of Pharmaceutical Education and Research, Hyderabad/India, IN

HRMS13_MO Use of Liquid Chromatography-High Resolution Mass Spectrometry (LC-HR/MS) in Bioanalytical Assays in Combination with Micro-Sampling of Biological Fluids Monica P. Cala Molina, Juan P. Rincon Pabon, Roland J.W. Meesters Universidad de los Andes, Bogota D.C., CO

HRMS14_TU Effect-Directed Isolation and Identification of Antibacterial Chelidonium Majus L. Components Ágnes M. Móricz1, Emilia Fornal2, Wioleta Jesionek3, Barbara Majer-Dziedzic4, Irena M. Choma3 1Plant Protection Institute CAR HAS, Budapest, HU; 2Chemistry Department and Laboratory of Separation and Spectroscopic Method Applications, Center for Interdisciplinary Research, The John Paul II Catholic University of Lublin, Lublin, PL; 3Department of Chromatographic Methods, University of Maria Curie-Skłodowska, Lublin, PL; 4Department of Veterinary Microbiology, University of Life Sciences, Lublin, PL

HRMS15_WE Structural Characterization of Biosimilar Therapeutic Monoclonal Antibody by LC- MS Marcell Olajos, Zsolt Gengeliczki, Krisztián Lenkey, Katalin Ganzler Gedeon Richter Plc., Budapest, HU

HRMS16_MO Frontal Affinity Chromatography Method for Quick Determination and Characterization of the Inhibitors of Tyrosinase in Complex Mixtures. The Comparison of Capillary-Format Miniaturized Approach and Modified 'Staircase' Frontal Affinity Chromatography. Aleksander Salwiński, Raphaël Delépée, David Da Silva, Benoît Maunit Institut de Chimie Organique et Analytique, UMR 73, Orléans, FR

HRMS17_TU Bio-Oil Oxygenated Compounds Analysis Using HPLC-MS Methods Martin Staš1, Josef Chudoba2, David Kubička1, Milan Pospíšil2 1Výzkumný ústav anorganické chemie, a.s., Ústí nad Labem, CZ; 2ICT Prague, Prague, CZ

HRMS18_WE Comparison between LC-Full Scan (Orbitrap) MS and Triple Quadrupole MS For Residue and Contaminant Screening in Fruits and Vegetables Pieter Dekker, Paul Zomer, Hans Mol RIKILT, Wageningen, NL

HRMS19_MO Profiling of N-Acylhomoserines and N-Acylhomoserine Lactones Using Automated Sample Preparation and UPLC-UHR-Tof-MS Michael Witting, Jenny Westphal, Juliano Fonseca, Philippe Schmitt-Kopplin Analytical BioGeoChemistry, Helmholtz Zentrum München, Neuherberg, DE

HRMS20_TU Ultra-Sensitive Determination of 5-Methylcytosine and 5-Hydroxymethylcytosine in Genomic DNA from Hepatocellular Carcinoma Tissues by Capillary Hydrophilic- Interaction Liquid Chromatography Coupled with Quadrupole Time-Of-Flight Mass Spectrometry Bi-Feng Yuan, Ming-Luan Chen, Fan Shen, Wei Huang, Jia-Hui Qi, Yu-Qi Feng, Song-Mei Liu Wuhan University, Wuhan, CN

HRMS21_WE QTOF Performance Improvements for Qualitative and Quantitative Multi-Target Pesticide Screening in Food Petra Decker1, Carsten Baessmann1, Oliver Raether1, Ilmari Krebs1, Ellen Scheerbaum2, Rebekka Loetterle2, Robert van der Heijden3 1Bruker Daltonik GmbH, Bremen, DE; 2Chemisches Veterinäruntersuchungsamt Stuttgart, Stuttgart, DE; 3Bruker Daltonics BV, Wormer, NL

Instrumentation INST01_MO Extending the Linear Dynamic Range of Absorbance Detectors Richard Andrews, Peyton Beals Waters Corp., Milford, MA, US

INST02_TU Quantitation of Surfactants in Samples by High Performance Liquid Chromatography and Corona Charged Aerosol Detection Bruce Bailey, Marc Plante, Ian Acworth Thermo Fisher Scientific, Chelmsford, US

INST03_WE Determination of Polyacrylic Acid in Boiler Water Using Size-Exclusion Chromatography with Charged-Aerosol Detection Bruce Bailey1, Xiaodong Liu2, Mark Tracy3 1Thermo Fisher Scientific, Chelmsford, US; 2Thermo Fisher Scientific, Sunnyvale, US; 3Thermo Fisher Scientific, Sunnvale, US

INST04_MO Extending the Limits of Operating Pressure of Normal and Narrow Bore Column Liquid Chromatography Instrumentation Ruben De Pauw1, Sebastiaan Eeltink1, Bart Degreef1, Harald Ritchie2, Gert Desmet1, Ken Broeckhoven1 1Vrije Universiteit Brussel, Brussels, BE; 2Thermo Fisher Scientiﬁc, Runcorn, GB

INST05_TU Automating pH Manipulation in Reversed-phase Method Development Aparna Chavali, Thomas E. Wheat, Patricia R. Mcconville Waters Corporation, Milford, Us

INST06_WE How to Control and Optimize Gradient Elution in Capillary Liquid Chromatography Enrique Javier Carrasco-Correa1, Agustín Acquaviva2, José Manuel Herrero-Martínez1, Guillermo Ramis-Ramos1 1Dept. de Química Analítica, Universidad de Valencia, Burjassot, Valencia, ES; 2Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, AR

INST07_MO Trace Determinations of Hexavalent Chromium in Soil Using Automated Extractions and Ion Chromatography Terri Christison1, Richard Jack1, Jinshui Che2, Lipika Basumallick1, Linda Lopez1 1Thermo Fisher Scientific, Sunnyvale, CA, US; 2Thermo Fisher Scientific, Shanghai, CN

INST08_TU Hyphenated HPLC-DAD-MS-SPE-NMR Interface for Identification of Phytochemicals: Application to an Orchid Extract Cyril Antheaume1, Veronika Cakova1, Nicole Rimlinger1, Patrick Wehrung1, Patrice André2, Frédéric Bonté Bonté2, Annelise Lobstein1 1Université de Strasbourg, Illkirch, FR; 2LVMH-Recherche, Saint-Jean de Braye, FR

INST09_WE HPLC NETWORK in Elucidation of Drug Metabolites Matthias Schiell, Markus Kohlmann Sanofi Deutschland GmbH, Frankfurt / Main, DE

INST10_MO Use of Variable Pixel Read Time to Improve Performance of Photodiode Array Detectors Joseph DiCesare PerkinElmer Corporation, Shelton, CT, US

INST11_TU Development of a Multidimensional Method for Screening Acetylcholinesterase Inhibitors in Crude Plant Extracts Luciana Freitas1, Alexandre Cruz2, Cardoso Carmen Lucia1 1University of Sao Paulo, Ribeirão Preto, BR; 2Sinc do Brasil, Ribeirão Preto, BR

INST12_WE Analysis of High- and Low-Dosed Vitamins in a Single Run Using the Agilent 1200 Infinity Series High Dynamic Range Diode Array Detector Solution Angelika Gratzfeld-Huesgen Agilent Technologies, Waldbronn, DE

INST13_MO Highly Sensitive Electrochemical Detector for a Miniaturized Liquid Chromatography System with a Microfluidic Chip Akihiko Ishida, Satoshi Yokogawa, Tatsuya Tanaka, Hirofumi Tani, Manabu Tokeshi Hokkaido University, Sapporo, JP

INST14_TU ‘CHROM-COMMANDER’, A Software Application for Combining Multiple HPLC Time Bases Sharing one HPLC Autosampler Unit under Dionex Chromeleon Integration Software Rainer Schmid1, Anton Luf1, Wolfhardt Freinbichler2 1Medical University of Vienna, Vienna, AT; 2MAYLAB Corp., Vienna, AT

INST15_WE Novel, Universal Approach for the Measurement of Natural Products in a Variety of Botanicals and Supplements Bruce Bailey, Marc Plante, Qi Zhang, David Thomas, Ian Acworth Thermo Fisher Scientific, Chelmsford, US

INST16_MO HPLC-DAD-SPE-NMR Methodology for Structure Elucidation of Secondary Organic Aerosol (SOA) Products Formed Through Aqueous Phase Photooxidation of Guaiacol Zoran Kitanovski1, Irena Grgić1, Alen Čusak2, Magda Claeys3 1National Institute of Chemistry, Ljubljana, SI; 2EN-FIST Center of Excellence, Ljubljana, SI; 3University of Antwerp, Antwerp, BE

INST17_TU Relative Response Factor Determination for an HPLC-UV Method Using Nuclear Magnetic Resonance Spectroscopy Lakshmy Nair, Christina Szabo, Catherine Quinn, Michael Koberda, Christopher Cullen, Yakov Genchanok, Karalyn Havel, Xiaohui Yang Baxter Healthcare, Round Lake, US

INST18_WE HPLC with Hyphenated Diode Array, Charged Aerosol and Electrochemical Detection Targeting Antioxidants in Apples Merichel Plaza1, Janes Kariuki2, Charlotta Turner1 1Lund University, Lund, SE 2University of Alberta, Camrose, CA

INST19_MO Recent Advances in Electrochemical Detection for UHPLC Nico Reinhoud, Hendrik-Jan Brouwer, Jean-Pierre Chervet Antec, Zoeterwoude, NL

INST20_TU In-Cell and External Hydrolysis of Biomass for Carbohydrate Determinations Using Automated Solvent Extraction Terri Christison, Richard Carlson, Lipika Basumallick, Linda Lopez Thermo Fisher Scientific, Sunnyvale, CA, US

INST21_WE Development and Evaluation of a Microscale UPLC System Thomas Wheat, Daniel Root, Patricia McConville Waters Corporation, Milford, MA, US

INST22_MO Computational Flow Study of the Optimal Design and Operating Conditions of the Flow Split Ring Used in Parallel Segmented Flow Columns Wim Smits, Gert Desmet Vrije Universiteit Brussel, Brussels, BE

INST23_TU Development of UHPLC Fingerprint Chromatographic Method for Phyllanthus Niruri Employing “Quality by Design” Approach Ricardo Sprenger1, Tiago Lourenço2, Fernando de Paula3, Quezia Cass1 1Federal University of São Carlos, São Carlos - São Paulo, BR; 2Waters Technologies of Brasil, Barueri - São Paulo, BR;3Waters Technologies of Brasil, São Carlos - São Paulo, BR

INST24_WE Quantitation of Trace Level Antibiotics in Minnesota River Water and Wastewater Effluent Using an Automated, Integrated Online-SPE LC-MS/MS System Dwight Stoll1, David Harmes1, Klaus Witt2 1Gustavus Adolphus College, St. Peter, US; 2Agilent Technologies, Waldbronn, DE

INST25_MO Ensuring Data Quality and Facilitating Rapid LC System Troubleshooting Using a QC Reference Material Mia Summers, Kenneth Berthelette, Kenneth Fountain, Euan Ross Waters Corp, Milford, MA, US

INST26_TU Intelligent System Emulation (ISET): New Possibilities in the Field of Method Development and Further Applications Heike Waechtler, Monika Dittmann, Klaus Witt, Konstantin Choikhet, Gerhart Metzler Agilent Technologies, Waldbronn, DE

INST27_WE Evaluation between UPLC and HPLC Analytical Methods for Characterizing Natural Dyestuffs Ana Serrano1, Maarten van Bommel1, Jessica Hallett2 1Rijksdienst voor het Cultureel Erfgoed, Amsterdam, NL; 2CHAM (Centre for Overseas History), Faculdade de Ciências Sociais e Humanas, Universidade Nova de Lisboa and Universidade dos Açores, Lisbon, PT

INST28_MO An Ion Mobility Detector for a High Pressure Liquid Chromatograph Hermann Wollnik, Gary Eiceman, Stephen Davila, Cheng-Hui Yuan New Mexico State University, Dept. Chem & Biochem., Las Cruces, NM, US

Latest Improvements in Development of Simple Splitless Gradient Liquid INST29_TU Chromatographic System for Micro and Nano Columns Jozef Šesták1, Vladislav Kahle2, Filip Duša2 1Faculty of Chemistry, Brno University of Technology, Brno, CZ; 2Institute of Analytical Chemistry of the ASCR, v.v.i., Brno, CZ

INST30_WE Multiple Switching Device for Complex and Accelerated HPLC Systems Rainer Schmid1, Goran Mitulovic1, Hans Mayrhofer2 1Dept. Laboratory Medicine, Medical University of Vienna, Vienna, AT; 2MAYLAB Corp., Vienna, AT

LC×LC LCLC01_MO Solid-Phase-Assisted Modulation for Two-Dimensional Liquid Chromatography Anna Baglai, Michelle Camenzuli, Peter Schoenmakers University of Amsterdam, Amsterdam, NL

LCLC02_TU Development and Application of 2D-LC-MS Analysis of Phospholipids Róbert Berkecz1, Viktor Szegedi2, Tamás Janáky1 1Department of Medical Chemistry, University of Szeged, Szeged, HU; 2Institute of Biochemistry, Biological Research Centre, Szeged, HU

LCLC03_WE 2D LC/MS Separation of Tryptic Peptides from Human Serum Proteins Using Reversed-Phase, Strong Cation Exchange, Weak Anion Exchange and Fused Core Fluorinated Stationary Phases Alexander Boichenko1, Natalia Govorukhina1, Ate van der Zee2, Rainer Bischoff1 1University of Groningen, Groningen, NL; 2University Medical Centre Groningen, Groningen, NL

LCLC04_MO Evaluation of Different Polar Stationary Phases for Hydrophilic Interaction Liquid Chromatography for in-depth Proteome Analysis Nhat Bui, Serena Di Palma, Albert J.R. Heck, Shabaz Mohammed Chemistry Department, Utrecht, NL

LCLC05_TU Optimization of Gradient Conditions in Two-dimensional Liquid Chromatography- Micellar Electrokinetic Capillary Chromatography Separations Petr Česla, Jana Váňová, Jan Fischer, Pavel Jandera University of Pardubice, Department of Analytical Chemistry, Pardubice, CZ

LCLC06_WE Advances in Hardware for Reliable and Informative 2D-LC Konstantin Choikhet, Herbert Anderer, Uwe Freisler, Klaus Witt Agilent Technologies Germany, Waldbronn, DE

LCLC07_MO LAM: Light-Induced Modulation for One- and Two-Dimensional Liquid Chromatography Henrik Cornelisson-van-de-Ven, Rudy J. Vonk, D.L.J. Broer, Peter J. Schoenmakers University of Amsterdam, Van 't Hoff Institute for Molecular Sciences, Amsterdam, NL

LCLC08_TU Two-Dimensional Liquid Chromatography (RP X HILIC) of Herbal Extracts Petra Dinisová1, Peter Schoenmakers2, Petr Česla1, Lenka Česlová1, Jan Fischer1 1University of Pardubice, Department of Analytical Chemistry, Pardubice, CZ; 2University of Amsterdam, Van’t Hoff Institute for Molecular Sciences, Amsterdam, NL

LCLC09_WE RPLC×RPLC of Dietary Supplements Using an 8-Port Valve Interface Mari Egeness1, Hernan Cortes2, Emily Hilder1, Robert Shellie1 1ACROSS, University of Tasmania, Hobart, AU; 2HJ Consulting LLC, Hobart, US

LCLC10_MO Comprehensive Two-Dimensional Liquid Chromatography (Lcxlc) Coupled with Mass Spectrometry for the Analysis of Fatty Alcohol Derivatives Victoria Elsner1, Michaela Wirtz2, David Melchior2, Oliver J. Schmitz3 1University of Wuppertal, Analytical Chemistry, Wuppertal, DE; 2BASF Personal Care and Nutrition GmbH, Düsseldorf, DE; 3University of Duisburg-Essen, Applied Analytical Chemistry, Essen, DE

LCLC11_TU Validation of a Rapid Lc-Ms/Ms Method for Determination of “Mad Honey” Toxins in Rhododendron Ponticum Muammer Kaplan, Öznur Karaoğlu, Elmas Öktem Olgun Tubitak Mrc Food Institute, Kocaeli, TR

LCLC12_WE Optimization of Gradients in Online Comprehensive Two-Dimensional Liquid Chromatography: Linear Solvent Strength Modeling of Peak-Capacity Rune Græsbøll1, Jan H. Christensen1, Nikoline J. Nielsen1, Hans-Gerd Janssen2 1University of Copenhagen, Frederiksberg C, DK; 2University of Amsterdam, Amsterdam, NL

LCLC13_MO Development of a Novel Two-Dimensional HPLC Method in Order to Analyze the Change of Topoisomer Pattern of Supercoiled Plasmid DNA during Fermentation Process Elisabeth Haller1, Marek Mahut1, Wolfgang Lindner1, Michael Laemmerhofer2 1University of Vienna, Vienna, AT; 2University of Tuebingen, Tuebingen, DE

LCLC14_TU Development of a Comprehensive Heart-cut 2D-LC-MS Method for the Analysis and Identification of New Steviol Glycosides in Stevia Rebaudiana Stijn Hendrickx1, Jari Rubbens1, Laura Van Den Bossche1, Wesley Vermaelen1, Etienne Jooken2, Amery Ruis2, Erwin Adams1, Sebastien Carpentier1, Deirdre Cabooter1 1KU Leuven, Leuven, BE; 2KHBO, Oostende, BE

LCLC15_WE HILIC×RP and RP×RP Comprehensive Two-Dimensional Liquid Chromatography Coupling 0.5 mm I.D. Monolithic Microcolumn and Core-Shell Column Tomáš Hájek, Magda Staňková, Pavel Jandera University of Pardubice, Pardubice, CZ

LCLC16_MO Separation Improvements with Orthogonal Chemistries and 2D LC Using Superficially Porous Columns Maureen Joseph1, William Long1, Jason Link1, Andrew Coffey2 1Agilent Technologies Inc, Wilmington, US; 2Agilent Technologies UK Ltd, Church Stretton, GB

LCLC17_TU New Method for the Determination of Pesticides and Insecticides in Water Samples by Large Volume Injection in 2-Dimensional HPLC Carolina Fernández-Ramos, Dalibor Šatínský, Petr Solich Faculty of Pharmacy, Charles University, Hradec Králové, CZ

LCLC18_WE Validation of Triphenylmethane Dyes and their Metabolites in Fish by Lc-Ms/Ms Öznur Karaoglu, Elmas Öktem Olgun, Muammer Kaplan tubitak Mrc Food Institute, Kocaeli, TR

LCLC19_MO Using of Shift Gradient in Second Dimension to Improve The Separation Space in Comprehensive Two-Dimensional Liquid Chromatography Duxin Li, Oliver J. Schmitz University of Duisburg-Essen, Essen, DE

LCLC20_TU The Qualification of Bi-Dimensional Chromatograms Based on Nearest Neighbors Distances Approach Witold Nowik1, Myriam Bonose2, Sylvie Heron2, Alain Tchapla2 1C2RMF, Paris, FR; 2Univ. Paris-Sud, LETIAM, Orsay, FR

LCLC21_WE Comprehensive Multi-Dimensional Liquid Chromatography Approaches for Application in Effect-Directed Analysis Xiyu Ouyang, Marja Lamoree, Pim Leonards Institute for Environmental Studies, VU University Amsterdam, Amsterdam, NL

LCLC22_MO Two-Dimensional LC and GC-MS: A Combination towards the Evaluation of Potential Geotracers in Saudi Arabian Oil Fields Saroj Panda, Khaled Arouri, Adnan Al-Hajji Saudi Aramco, Dhahran, SA

LCLC23_TU Comparison of Commercial Software Approaches for Quantitation in Two- Dimensional Liquid Chromatography Melissa Phillips, Mallory Morris, Benjamin Place, Catherine Rimmer, Lane Sander NIST, Gaithersburg, US

LCLC24_WE Single-Pumped Heart-Cut Bi-Dimensional Liquid Chromatography Applied to the Determination of Surfactants and Block Copolymers Containing Ethylene Oxide Aaron Escrig-Domenech, Miriam Beneito-Cambra, Ernesto F. Simo-Alfonso, Guillermo Ramis- Ramos University of Valencia, Fac. of Chemistry, 4600 Burjassot, ES

LCLC25_MO Data Analysis Workflow for Comprehensive Two-Dimensional Liquid Chromatography (LCxLC) Stephen Reichenbach1, Satya Vijay Devarakonda1, Edgar Naegele2, Qingping Tao3, Gerd Vanhoenacker4 1University of Nebraska, Lincoln, US; 2Agilent Technologies, Waldbronn, DE; 3GC Image, LLC, Lincoln, US; 4Research Institute for Chromatography, Kortrijk, BE

LCLC26_TU Improving Resolution and Sensitivity in Multidimensional Chromatography Daniel Root, Aparna Chavali, Thomas Wheat Waters Corporation, Milford, US

LCLC27_WE On-Line Lcxlc Hyphenation by a Machine Vision Controlled Solvent Evaporation Interface Jan-Willem Schoonen, Paul Vulto, Heiko van der Linden, Thomas Hankemeier Netherlands Metabolomics Centre, Leiden, NL

LCLC28_MO Data Pre-Processing Technique for Fast Multidimensional High Performance Liquid Chromatography Paul Stevenson, Danielle Bassanese, Xavier Conlan, Neil Barnett Deakin University, Geelong, Victoria, AU

LCLC29_TU Drug Target Deconvolution in Cancer Cells Using Liquid Chromatography, Fluorescence Polarization and Mass Spectrometry Tore Vehus1, Steven Ray Wilson1, Elsa Lundanes1, Tyge Greibrokk1, Stefan Krauss2 1University of Oslo, Oslo, NO; 2Oslo University Hospital, Oslo, NO

LCLC30_WE HPLC Online SPE Method Analysis Morphine in Compound Glycyrrhiza Preparation Yao Xiao, Lang Li Agilent Technologies (China) Co.,Ltd., Beijing, CN

LCLC31_MO Highly Orthogonal Off-Line Hsax Fractionation for the Analysis of Alternatively Activated Macrophages Robert van Ling1, Maria Stella Ritorto2, Manman Guo2, Matthias Trost2, Ken Cook3 1Thermo Scientific, Breda, NL; 2MRC Phosphorylation Unit, University of Dundee, Dundee, GB; 3Thermo Scientific, Hemel Hempstead, GB

LCLC32_TU Capabilities and Limitations of Fast Online LCxLC; Challenges of Coupling MS to LCxLC Imad A. Haidar Ahmad, Brian B. Barnes, Robert C. Allen, and Peter W. Carr Department of Chemistry, University of Minnesota, Minneapolis, MN, USA

LC-MS Principles LCMS01_MO Analysis of Cavitand Derivatives with High Performance Liquid Chromatography – Mass Spectrometry Endre Bartó, Ibolya Prauda, Viktor Sándor, Ferenc Kilár, Attila Felinger, Ibolya Kiss University of Pécs, Pécs, HU

LCMS02_TU Influence of Column Internal Diameter Upon Chromatographic Performance and Detection Sensitivity in Ultra High Performance LC/MS Analysis Stephan Buckenmaier, Monika Dittmann Agilent Technologies, Waldbronn, DE

LCMS03_WE Experimental Evidence and Characterization of Cobalt(I) Complexes Relevant in Regioselective Diels-Alder Reactions. A Gas Phase Study Lukas Fiebig1, Julian Kuttner2, Gerhard Hilt2, Martin C. Schwarzer2, Gernot Frenking2, Hans- Günther Schmalz1, Mathias Schäfer1 1Department of Chemistry, Institute of Organic Chemistry, Köln, DE; 2Fachbereich Chemie, Philipps-University of Marburg, Marburg, DE

LCMS04_MO Fluorous Derivatization Technique for Matrix Effect-Free LC-MS/MS Analysis: Application to Biogenic Amine Analysis Tadashi Hayama1, Yohei Sakaguchi1, Erina Tamashima1, Kenichiro Todoroki2, Hideyuki Yoshida1, Hitoshi Nohta1, Masatoshi Yamaguchi1 1Fukuoka University, Fukuoka, JP; 2University of Shizuoka, Shizuoka, JP

LCMS05_TU Negative Electrospray Ionisation of Fluorotelomer Alcohols (FTOH) and FTOH- Derived Fluoroacrylate Surfactants Using Liquid Chromatography Coupled to Accurate (Tandem) Mass Spectrometry Xenia Trier1, Jan H. Christensen2, Wilfried M. A. Niessen3 1National Food Institute, Technical University of Denmark, Søborg, Copenhagen, DK; 2Analytical Chemistry group, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, DK; 3Hyphen MassSpec, Leiden and AIMMS Division of BioMolecular Analysis, VU University Amsterdam, Amsterdam, NL

LCMS06_WE Novel Methylated Ornithine Lipids in Russian Wetland Planctomycetes Ellen Hopmans1, Elisha Moore1, Irene Rijpstra1, Laura Villanueva1, Svetlana Dedysh2, Hans Wienk3, Frans Schoutsen4, Jaap Sinninghe Damsté1 1NIOZ Royal Netherlands Institute for Sea Research, Den Burg, NL; 2S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, RU; 3Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, NL; 4Thermo Fisher Scientific, Breda, NL

LCMS07_MO Analysis of Steroidal Compounds in Wastewater by Liquid Chromatography Mass Spectrometry: Comparison of Different Ionization Techniques Matias Kopperi, Raymundo Lizcano, Kari Hartonen, Marja-Liisa Riekkola University of Helsinki, Helsinki, FI

LCMS08_TU Reduction of Matrix Effects and Improvement of Sensitivity during Determination of Two Polar Pesticide Metabolites by Using LC-MS Sebastian Kowal1, Friedrich Werres1, Peter Balsaa1, Torsten Schmidt2 1IWW Water Centre, Mülheim an der Ruhr, DE; 2university duisburg essen, Essen, DE

LCMS09_WE Permanent Post-Column Infusion as a Challenge of Matrix Effect Compensation in Quantitative LC -MS/MS Analysis of Pharmaceuticals in Sewage of Dresden Julia Rossmann, Robert Gurke, Sara Schubert, Reinhard Oertel, Wilhelm Kirch Institut for Clinical Pharmacology, Technical University, Dresden, DE

LCMS10_MO Identification of Imidacloprid Metabolites in Onion (Allium Wakegi) Using High Resolution Mass Spectrometry and Accurate Mass Tools Michael Thurman1, Imma Ferrer1, Paul Zavitsanos2, Jerry Zweigenbaum2 1University of Colorado, Boulder, Colorado, US; 2Agilent Technologies, Inc., Wilmington, DE, US

LCMS11_TU Fluorous Derivatization Technique for Matrix Effect-Free LC-MS/MS Analysis: Application to Biogenic Amine Analysis Tadashi Hayama1, Yohei Sakaguchi1, Erina Tamashima1, Kenichiro Todoroki2, Hideyuki Yoshida1, Hitoshi Nohta1, Masatoshi Yamaguchi1 1Fukuoka University, Fukuoka, JP; 2University of Shizuoka, Shizuoka, JP

LCMS12_WE Mass Spectrometric Analysis of the Interaction between Transferrin and Gadolinium Kristina Wentker, Olga Reifschneider, Christoph A. Wehe, Uwe Karst University of Münster, Münster, DE

LCMS13_MO Determination of Trace Amounts of Bisphenol A in Human Serum by Liquid Chromatography Coupled with Tandem Mass Spectrometry Kamila Wilczewska1, Andrzej Wasik1, Aleksandra Rutkowska2, Paweł Kubica1, Agata Kot-Wasik1, Jacek Namieśnik1 1Gdańsk University of Technology, Faculty of Chemistry, Gdańsk, PL; 2Medical University of Gdańsk, Department of Clinical and Exp, Gdynia, PL

LCMS14_TU Quantitative, Antibody-Free LC-MS/MS Analysis of Recombinant Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL) in Serum Daniel Wilffert1, Carlos R. Rodrigues dos Reis1, Jos Hermans1, Natalia Govorukhina1, Tushar Tomar2, Steven de Jong2, Wim J. Quax1, Nico C. van de Merbel1, Rainer Bischoff1 1University of Groningen (RUG), Groningen, NL; 2Universitair Medisch Centrum Groningen (UMCG), Groningen, NL

LC-MS of Small Molecules LCSM01_MO Anthocyanin Constituents of Blue and Purple Wheat and their Thermal Stability during Baking Alen Albreht1, Irena Vovk1, Mihaela Skrt2, Nataša Poklar Ulrih3, Pavel Bartl4, Bohuslava Tremlova4 1National Institute of Chemistry;EN-FIST Centre of Excellence, Ljubljana, SI; 2University of Ljubljana, Biotechnical Faculty, Ljubljana, SI; 3University of Ljubljana, Biotechnical Faculty; Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Ljubljana, SI; 4University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Brno, CZ

LCSM02_TU LC-MS/MS Method Development for the Determination of Triazines and Triazoles Migrating from Food Packaging Materials Zsolt Bodai, Márton Novák, Zsuzsanna Eke Eötvös Loránd University - EKOL, Budapest, HU

LCSM03_WE How to Use Column Characterization Databases to Select Similar/Dissimilar Reversed Phase Columns for Liquid Chromatography? Endler Borges1, Melvin Euerby2 1Universidade Estadual de Campinas, Campinas, BR; 2Strathclyde Institute of Pharmacy and Biomedical Sciences, Glasgow, GB

LCSM04_MO LC/MS/MS Measurement of Kynurenine Metabolites from Tissue Samples in the Study of Neurological Disorders Associated with Neuroinflammation David Budac, Cayla Vila, Danielle LaBarbiera, Manuel Cajina, Guiying Li, Stephanie Bisulco, Anna Lee, Brian Campbell Lundbeck Research USA, Paramus, NJ, US

LCSM05_TU Determination of 74 Pesticides in Vegetables by Ultra High Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry Gao Hui1, Yueqi Li1, Chao Ma1, Hongyuan Hao1, Guixiang Yang1, Taohong Huang2, Shin-ichi Kawano2, Yuki Hashi2 1Shimadzu (China) Co.,Ltd., Beijing, CN; 2Shimadzu (China) Co.,Ltd., Shanghai, CN

LCSM06_WE Semi-Preparative LC-UV Method for Purification of Compounds by Stacked Injections Josiane de Oliveira Cardoso1, Tiago Venâncio1, Rosângela Gonçalves Peccinini2, Ivan da Rocha Pitta3, Maria C. A. Lima4, Regina Vincenzi de Oliveira1 1Federal University of São Carlos, São Carlos, BR; 2State University of São Paulo, Araraquara, BR; 3Federal University of Pernambuco, Recife, BR; 4Federal University of Pernambuco, Brazil, Recife, BR

LCSM07_MO Development of a Novel Method for the Extraction of Norbixin from Whey and its Subsequent Quantification via High Performance Liquid Chromatography Rachel Campbell1, Ilco Boogers2, Mary Anne Drake1 1NC State University, Raleigh, US; 2DSM Biotechnology Centre, Delft, NL

LCSM08_TU High-Through-Put LCMS Using Active Flow Technology Chromatography Columns Tony Edge, Dominic Foley, Luisa Pereira Thermo Fisher Scientific, Runcorn, GB

LCSM09_WE Water Soluble Biopolymers as Sampling Medium for Dried Matrix Spot. Lars Erik Eng Eibak, Astrid Gjelstad, Knut Einar Rasmussen, Stig Pedersen-Bjergaard University of Oslo, Oslo, NO LCSM10_MO Development of a SPE-HPLC-ESI+-Scheduled-MS/MS -Method for the Qualification and Quantification of Pharmaceuticals in an Urban Sewage System in Germany Robert Gurke, Julia Rossmann, Sara Schubert, Reinhard Oertel, Wilhelm Kirch Institute of Clinical Pharmacology, TU Dresden, Dresden, DE

LCSM11_TU Development of a Liquid Chromatography Ion Trap Mass Spectrometry (LC-ITMS) Method for the Analysis of Nimbolide in Rat Liver Microsomes Josiane de Oliveira Cardoso, Bianca Ferreira da Silva, Tiago Venâncio, Regina Vincenzi de Oliveira Federal University of São Carlos, Anapolis, BR

LCSM12_WE Analysis of Calix[4]resorcinarene Cavitand with High Performance Liquid Chromatography – Mass Spectrometry Ibolya Prauda, Endre Bartó, Viktor Sándor, Ferenc Kilár, Ibolya Kiss, Attila Felinger University of Pécs, Pécs, HU

LCSM13_MO LC-MS/MS Method for Determination of Anti-Cancer Agent Bp4eT and its Main Phase I Metabolites in Rat Plasma Petra Kovarikova1, Jan Stariat1, Vlasta Suprunova1, Vit Sestak1, Des R. Richardson2 1Faculty of Pharmacy, Charles University in Prague, Hradec Kralove, CZ; 2Department of Pathology and Bosch Institute, University of Sydney, Sydney, AU

LCSM14_TU Comparison of LC and LC-MS Methods for the Determination of Hydroxylated Polycyclic Aromatic Hydrocarbons in Different Matrices Jutta Lintelmann1, Evelyn Huebner1, Juergen Wendt2, Ralf Zimmermann1 1Helmholtz Zentrum Muenchen, Neuherberg, DE; 2Leco Instrumente GmbH, Moenchengladbach, DE

LCSM15_WE Determination of Illicit Drug Compounds from Biological Samples with Bio- Compatible SPME Fibers and HPLC/MS Frank Michel1, Craig R. Aurand2, Robert E. Shirey2, Yong Chen2, Leonard M. Sidisky2 1Sigma-Aldrich, Taufkirchen, DE; 2Supelco/Sigma-Aldrich, Bellefonte, US

LCSM16_MO Identification of Mauveine in Victoria Postage Stamps by HPLC-DAD-MS/MS M. Conceição Oliveira1, Ana Dias1, Sérgio Seixas Melo2 1Centro Química Estrutural, Instituto Superior Técnico, UTL, Lisboa, PT; 2Department of Chemistry, University of Coimbra, Coimbra, PT

LCSM17_TU A Novel Derivatization Method for the Determination of Fosfomycin Trometamol in Human Plasma by LC-ESI-MS Via Phase Transfer Catalyzed Derivatization Theodora Papakondyli, Aikaterini Gremilogianni, Nikolaos Megoulas, Michael Koupparis University of Athens, Athens, Gr

LCSM18_WE Method Development Strategy for Derivatization LC/ESI/MS Riin Rebane, Koit Herodes, Maarja-Liisa Oldekop University of Tartu, Tartu, EE

LCSM19_MO Optimization of a LC-MS2 Method for Analysis of Iminosugars from Natural Sources Sonia Rodríguez-Sánchez, Jesús Eduardo Quintanilla-López, Ana Cristina Soria, Maria Luz Sanz IQOG (CSIC), Madrid, ES

LCSM20_TU Evaluation of Recent Analytical Development in Bioanalysis in the Latest Decade. A Case Study: The Assay of the Budesonide Epimers in Human Plasma Bruno Streel, Cahay Bernard, Klinkenberg Régis, Traina Karl Galephar, Marche en Famenne, BE

LCSM21_WE The Development of Fast, Sensitive and Selective Multistatin Method for the Determination of Statins, their Metabolites and Interconvesion Products in Human Serum Hana Vlckova1, Ondrej Novak2, Pavel Svoboda1, Petr Solich1, Lucie Novakova1 1Charles university in Prague, Faculty of Pharmacy, Hradec Kralove, CZ; 2Palacky University and Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, CZ

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LCSM22_MO Sample Preparation and HPLC/MS Determination of Veterinary Drugs in Milk and Animal Tissue Frank Michel1, Emily Barrey2, Katherine K. Stenerson2, Olga Shimelis2, Michael Ye2 1Sigma-Aldrich, Taufkirchen, DE; 2Supelco/Sigma-Aldrich, Bellefonte, US

LCSM23_TU The Use of Microflow UHPLC as a Way to Solvent Usage in Pesticide Screening of Food Samples by LC-MS/MS Stephen Lock AB SCIEX, Pudsey, GB

LCSM24_WE Screening of 25-OH Vitamin D2,D3 Using Shimadzu LCMS-8040 Giancarlo La Marca1, Sabrina Malvagia2, Stefano Zaza3 1Mass Spectrometry and Pharmacology Laboratory, A. Meyer Chil, Florence, IT; 2Mass Spectrometry and Pharmacology Laboratory, A. Meyer Children’s Hospital Pediatric Neurology Unit and Laboratories, University of Florence, Florence, IT; 3Shimadzu Italia, Milan, IT

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Lipids LPDS01_MO Detection and Structural Elucidation of Esterified Oxy-Lipids in Human Synovial Fluid by ESI-FTICRMS and LC-IT-MS3: Detection of Esterified Hydroxylated Docosapentaenoic Acid Containing Phospholipids Hulda Jónasdóttir, Simone Nicolardi, Rico Derks, Magnus Palmblad, Andreea Ioan-Facsinay, Rene Toes, Yuri van der Burgt, Andre Deelder, Oleg Mayboroda, Martin Giera LUMC, Leiden, NL

LPDS02_TU A Global Method for the Quantitation and Characterization of Lipids by High Performance Liquid Chromatography and Corona Charged Aerosol Detection Bruce Bailey, Marc Plante, Ian Acworth Thermo Fisher Scientific, Chelmsford, US LPDS03_WE Mass Spectrometric Investigation of Rhamnolipids Beate Behrens, Jeannine Engelen, Heiko Hayen University of Wuppertal, Department of Food Chemistry, Wuppertal, DE

LPDS04_MO Employment of a Stop-Flow Technique in Comprehensive Two-Dimensional Liquid Chromatography for Fish Oil Analysis Marco Beccaria, Giuseppe Sullini, Paola Donato, Francesco Cacciola, Ivana Bonaccorsi, Antonella Cotroneo, Paola Dugo, Luigi Mondello University of Messina, Messina, IT

LPDS05_TU Method Development for Complex Samples in Achiral SFC: Vegetable Oils as A Case Study Eric Lesellier1, Alessandra Lopes de Oliveira2, Anna Latos1 1ICOA, Orléans, FR; 2FZEA/UPS, Sao Paulo, BR

LPDS06_WE Investigating the Presence of Specialized Pro-Resolving Lipid Mediators in Rheumatoid Arthritis Patients Using an Advanced LC-MS/MS Platform Hulda Jónasdóttir, Andreea Ioan-Facsinay, Oleg Mayboroda, Rene Toes, Andre Deelder, Martin Giera LUMC, Leiden, NL

LPDS07_MO Nontargeted Quantitation of Non-Polar Lipids Blanka Červená, Eva Cífková, Miroslav Lísa, Michal Holčapek University of Pardubice, Pardubice, CZ

LPDS08_TU Separation of Hydrocarbons by Silver Ion High-Performance Liquid Chromatography Petra Horká1, Vladimír Vrkoslav1, Karolina Pecková2, Josef Cvačka1 1Institute of Organic Chemistry and Biochemistry AS CR, v.v.i, Prague, CZ; 2Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Prague, CZ

LPDS09_WE Application of Sub-2µm particle CO2-based Chromatography Coupled to Mass Spectrometry for Comprehensive and Targeted Analysis of Lipids in Cottonseed Extracts Michael Jones1, Giorgis Isaac2, Vladimir Shuleav3, Carolina Salazar3 1King's College, London, London, GB; 2Waters, Milford, MA, US; 3University of Northern Texas, Denton, TX, US

LPDS10_MO Comprehensive GC-MS Analysis of Fatty Acids and Sterols Using Sequential One-Pot Silylation Dick-Paul Kloos1, Emmanuel Gay2, Henk Lingeman1, Oleg Mayboroda2, Wilfried Niessen1, André Deelder2, Martin Giera1 1Free University Amsterdam, Amsterdam, NL; 2LUMC, Leiden, NL

LPDS11_TU Lipidomic Characterization of Vital Porcine Organs Using HILIC-HPLC/ESI-MS Eva Cífková, Michal Holčapek, Miroslav Lísa University of Pardubice, Pardubice, CZ

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LPDS13_MO Development of an Analytical Strategy for the Analysis of Different Classes of Solutes in Reversed Phase Liquid Chromatography. Fast Determination of Isoeluotropic Conditions by Changing the Stationary Phase in Aqueous and Non-Aqueous Liquid Chromatography. Alain Tchapla1, Tamba Sompila Arnaud2, Didier Charbonneau3, Marie Geneviève Maloumbi4, Sylvie Heron1 1Univ.Paris Sud/Groupe de Chimie Analytique de Paris-Sud, Orsay, FR; 2Univ. Paris-Sud/Groupe de Chimie Analytique de Paris-Sud, Orsay, FR; 3Interchim R & D, Montluçon, FR; 4Univ.M. Ngouabi/ UC2V, Brazzaville, CG

LPDS14_TU Glycerophospholipids Analysis by Two-Dimensional Liquid Chromatography Coupled with a Triple Quadrupole Mass Spectrometer Daisuke Nakayama, Jing Dong, Keiko Yamabe, Takashi Suzuki, Yoshihiro Hayakawa, Junichi Masuda, Okiyuki Kunihiro Shimadzu Corporation, Kyoto, JP LPDS15_WE Detection and Identification of Natural Anti-Oxidants in Edible Oils Using Liquid Chromatographic Fractionation with Off-Line Effect-Based Detection Ruud Poort, Herrald Steenbergen, Hans-Gerd Janssen Unilever Research and Development Vlaardingen, Vlaardingen, NL

LPDS16_MO Identification and Quantification of Triglycerides by UHPSFC-UV-ELSD: Classification of Vegetable Oils with Cluster Analysis Eric Lesellier, Anna Latos, Caroline West ICOA, UMR 7311, Université Orélans, Orléans, FR

LPDS17_TU Microdetermination of Labile Estrogen-O-Quinones and their Cysteine/Glutathione Conjugates by High Performace Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry Kouwa Yamashita, Miki Shiratori, Emi Kondo, Sachiko Komatsu, Hajime Kato, Ken-ichi Ohno Tohoku Pharmaceutical University, Sendai, JP

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Mass Spectrometry MASS01_MO Separation of Steroid Isomers by Ion Mobility Mass Spectrometry Linda Ahonen1, Maíra Fasciotti2, Gustav Boije af Gennäs1, Tapio Kotiaho1, Marcos Eberlin2, Risto Kostiainen1 1University of Helsinki, Helsinki, FI; 2University of Campinas, Campinas, BR

MASS02_TU Combining Mass and UV Online Detection in Flash Chromatography Mark Allen1, Lawrence Klecha2, Nigel Sousou2, Ben Trumbore2 1Advion Ltd., Harlow, GB; 2Advion Inc,, Ithaca, US

MASS03_WE MISPE-LC-Tof Sorbent Selective Determination of Triazine Compounds in Corn Felipe Andrade, Carlos Nazario, Álvaro Santos Neto, Fernando Lanças Institute of Chemistry São Carlos/University of São Paulo, São Carlos-SP, BR

MASS04_MO Rapid and Sensitive Liquid Chromatography Triple Quadrupole Mass Spectrometry (LC-QQQ) Method for the Determination of Gemifloxacin in Human Urine and its Pharmacokinetic Application Adnan Kadi, Mohamed Attwa, Ali Abdelhameed Department of Pharmaceutical Chemistry, King Saud University, Riyadh, SA

MASS05_TU Electrospray Ionization Mass Spectrometry of Didodecylpolysulfides Using Silver(1) Complexation Christie Bowden, Sriraj Srinivasan, Gary Smith Arkema Inc., King of Prussia, PA, US

MASS06_WE Micro-Scaled HPLC-SPE/MS for Isolation of Metabolites Obtained by Micro-Batch Synthesis Using Recombinant Human CYP450-1A1 and their Structure Elucidation by NMR Thierry Délémonté1, Reiner Aichholz1, Joachim Blanz1, Jérôme Dayer1, Werner Gertsch1, David Pearson1, Philippe Ramstein1, Alexandre Luneau2, Lukas Oberer2, Dirk Behnke3, David Carcache3, Fabian Kurt Eggimann3, Matthias Kittelmann3, Stephan Lütz3, Alexandra Vargas3 1-3Novartis Pharma AG, Novartis Institutes for Biomedical Research, CH-4053 Basel, Switzerland (1Metabolism and Pharmacokinetics, 2Analytical Sciences, 3Global Discovery Chemistry)

MASS07_MO Determination of 23 Synthetic Cannabinoids in Human Hair by Ultra-High Performance Liquid Cromatography Tandem Mass Spectrometry Daniele Di Corcia, Alberto Salomone, Clemente Luciano, Enrico Gerace, Marco Vincenti Centro Regionale Antidoping, Orbassano (TO), IT

MASS08_TU Different Approaches for the Online Determination of Endocrine Disruptors and Related Compounds in Water Samples by Equantm and Turbulent-Flowtm Chromatography-LC-MS/MS Marina Gorga1, Mira Petrovic2, Damià Barceló3 1CSIC-IDAEA, Barcelona, ES; 2ICRA and ICREA, Girona, ES; 3CSIC and ICRA, Barcelona, ES

MASS09_WE Ultra Fast UHPLC-LCMSMS Method Development in Clinical Drug Monitoring Anja Gruening1, Brigitte Dr. Richrath1, Klaus Dr. Bollig2, Sven Vedder1, Robert Ludwig1 1Shimadzu Europa GmbH, Duisburg, DE; 2Shimadzu Deutschland GmbH, Duisburg, DE

MASS10_MO Supercritical Carbon Dioxide Extraction and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Analysis of Pyrolysis Bio-oil Yehua Han State Key Laboratory of Heavy Oil Processing, Beijing, CN

MASS11_TU Challenges and Opportunities of Developing Ultra-Low Dose Inhalation Products. Combination of Ultraviolet (UV) and Mass Spectrometry (MS) Detection. Viktoria Hartung, Phillip Krüger Almirall Sofotec GmbH, Bad Homburg, DE

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MASS12_WE Determination of 25-hydroxy Vitamin D in Human Breast Milk Eva Kasalová1, Jiří Plíšek2, Jana Aufartová2, Lenka Krčmová2, Dagmar Solichová3, Petr Solich1 1Fac. of Pharmacy, Charles University, Dept. Anal. Chem., Hradec Králové, CZ; 2Fac. of Pharmacy, Charles University, Dept. Anal. Chem. and III.Internal Gerontometabolic Clinic, University Hospital, Hradec Králové, CZ; 3III.Internal Gerontometabolic Clinic, University Hospital, Hradec Králové, CZ

MASS13_MO Detection and Quantification of Electrochemically Generated Metabolites of Thyroxine (T4) by means of LC-ESI-MS and LC-ICP-MS Chun Kong Mak1, Christoph A. Wehe2, Michael Sperling2, Uwe Karst2 1NRW Graduate School of Chemistry, University of Muenster, Muenster, DE; 2University of Muenster, Muenster, DE

MASS14_TU Determination of 11 Coccidiostats in Feeds by UPLC-MS/MS Cinzia Civitareale1, Paolo Stacchini1, Marilena Muscarella2, Maurizio Fiori1 1Istituto Superiore di Sanità, Roma, IT; 2Istituto Zooprofilattico Sper. Puglia e Basilicata, Foggia, IT

MASS15_WE High-Throughput Bioanalytical LC-MS/MS Methods Applied to Snapshot PK and Full PK Studies in Anti-Trypanosomal Drug Development Mouhssin OUFIR1, Kirsten Gillingwater2, Tanja Wenzler3, Daniela Eigenmann1, Reto Brun3, Matthias Hamburger1 1Division of Pharmaceutical Biology, University of Basel, Basel, CH; 2Parasite Chemotherapy, Swiss TPH, University of Basel, Basel, CH; 3Parasite Chemotherapy, Swiss TPH, University of Base, Basel, CH

MASS16_MO Multivariate Analysis of a Herbal Medicinal Product Using UPLC-QTOF-MS Mark Powell1, Margaret Maziarz2, Michael D. Jones2, Warren Potts2, Kate Yu2 1Quay Pharmaceuticals, Deeside, Flintshire, GB; 2Waters Corporation, Milford, MA, US

MASS17_TU UHPLC Coupled to Mass Spectrometry Applied on the Phenolic Fraction Characterization of Eucalyptus Grandis, E. Urograndis (E.Grandis X E. Urophylla) and E. Maidenii Woods Sónia Santos1, Carla Vilela, Carmen Freire, Carlos Pascoal Neto, Armando Silvestre University of Aveiro/CICECO, Aveiro, PT

MASS18_WE Quantification of the Endogenous Peptide Ghrelin in Human Plasma by LC-SRM/MS and LC-SRM3/MS Jonathan Sidibé, Emmanuel Varesio, Gérard Hopfgartner University of Geneva, Geneva, CH MASS19_MO LC/MS/MS Study on Bioactivation of a Cardioprotective Drug Dexrazoxane in Vivo Jan Stariat1, Petra Kovarikova1, Jan Bures1, Eduard Jirkovsky2, Olga Popelova-Lencova2, Martin Sterba2 1Faculty of Pharmacy in Hradec Kralove, Charles University, Hradec Kralove, CZ; 2Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, CZ

MASS20_TU Development of a Validated LC-MS/MS Method to Monitor a Traditional Chinese Medical Formulation of Ling-Gui-Zhu-Gan Decoction Chang Yang Tan1, Lie-Chwen Lin2, Tung-Hu Tsai1 1National Yang-Ming University, Taipei, TW; 2National Research Institute of Chinese Medicine, Taipei, TW

MASS21_WE Development and Validation of a Gas Chromatography Mass Spectrometry Method for Analysis of Phytoestrogens and Mycotoxins in Estuarine Water Samples Cláudia Ribeiro1, Ana R Ribeiro2, Maria E Tiritan3 1CICS-ISCSN-CESPU, Gandra - Paredes, PT; 2CICS-ISCSN-CESPU, CBQF-ESB-UCP, CEQUIMED-UP, Gandra - Paredes, PT; 3CICS-ISCSN-CESPU, CEQUIMED-UP, Gandra - Paredes, PT

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MASS22_MO Comparison of Two Multi-Residue Methods Using Solid-Phase Extraction (SPE) and Direct-Injection (DI) for the Determination of Pesticide Residues in Wines by LC- MS/MS Despina Tsipi-Stefanitsis1, Helen Botitsi1, Spyros Antoniou1, Sotirios Katsikis2, Anastasios Economou2 1General Chemical State Laboratory, Athens, GR; 2University of Athens, Athens, GR

MASS23_TU Online ESI-MS Analysis of HPTLC-Separated Compounds with a New Compact Single Quadrupole Mass Spectrometer Kees Vlak1, Frank Porbeck1, Ines Klingelhoefer2, Gerda Morlock2 1Advion Ltd., Harlow, GB; 2Justus-Liebig-University, Giessen, DE

MASS24_WE Combining Mass and UV Spectral Information with Systematic Mobile Phase Screening for Chromatographic Method Development Thomas Wheat, Patricia McConville Waters Corporation, Milford, MA, US

MASS25_MO A Simple and Rapid Method for Simultaneous Determination of Food Additives in Beverages and Soft Drinks Kamila Wilczewska, Anna Jakimska, Weronika Hewelt-Belka, Andrzej Wasik, Agata Kot-Wasik, Jacek Namieśnik Gdańsk University of Technology, Faculty of Chemistry, Gdańsk, PL

MASS26_TU Molecularly Imprinted Polymer Sorbent for Analysis of Triazines by MEPS-LC-ESI- ToF Felipe Andrade, Álvaro Santos Neto, Fernando Lanças Institute of Chemistry São Carlos, University of São Paulo, São Carlos-SP, BR

MASS27_WE Determination of Four Immunosuppressive Drugs in Whole Blood Using MEPS and LC-MS/MS Allowing Automated Sample Work-Up and Analysis Rana Said, Olof Beck Karolinska institute, Huddinge, SE

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Metabolomics META01_MO Search for Markers of Bladder Cancer with a Metabolomic Approach Antonia García-Fernández1, Juliana Alberice2, Alberto Amaral3, Nuria Malats3, Coral Barbas1 1CEMBIO Facultad de Farmacia, Universidad CEU San Pablo, Boadilla del Monte, ES; 2IQSC, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, BR; 3CNIO Centro Nacional de Investigaciones Oncológicas, Madrid, ES

META02_TU Metabolic Profiling by Hydrophilic Interaction Ultra-Performance Liquid Chromatography Time-Of-Flight Mass Spectrometry in the Study of Chronic Kidney Disease Jente Boelaert1, Griet Glorieux2, Eva Schepers2, Nathalie Neirynck2, Raymond Vanholder2, Frédéric Lynen1 1Separation Science Group, Ghent University, Gent, BE; 2Renal Division, Ghent University, Gent, BE

META03_WE Identification of Metabolites of Davidiin, a Potential Antitumor Ellagitannin, Transformed by Rat Intestinal Bacteria in Vitro, Based on Liquid Chromatography/Quadrupole Ion Trap/Time-Of-Flight Mass Spectrometry Analysis Ma Chao1, Fu Jie2, Ma Jingyi2, Wang Yan2, Gao Hui2 1Shimadzu Global COE, Shimadzu (China) Co., Ltd., Beijing, CN; 2Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, China, Beijing, CN

META04_MO High Volume Injections of Biological Samples for Sensitive Metabolite Profiling Russell Jones, Marc Bockx, Filip Cuyckens Janssen R&D, Beerse, BE

META05_TU The Pitfall of Cytokinin Nucleotide Determination by LC/MS Petre I. Dobrev, Václav Motyka, Radomíra Vaňková Institute of Experimental Botany AS CR, Prague, CZ

META06_WE Comparison of an Optimized LC-MS Method with Direct Infusion MS for Metabolomic Analysis in Human Cell Cultures Ines C. Forstenlehner, Volker Neu, Christian G. Huber University of Salzburg, Salzburg, AT

META07_MO LC- and GC-MS Based Non-Targeted Metabolomics in the Study of Chronic Kidney Disease Jente Boelaert1, Ruben t' Kindt2, Lucie Jorge2, Griet Glorieux3, Eva Schepers3, Nathalie Neirynck3, Raymond Vanholder3, Frédéric Lynen1, Pat Sandra2, Koen Sandra2 1Separation Science Group, Ghent University, Gent, BE; 2Metablys, Research Institute for Chromatography, Kortrijk, BE; 3Renal Division, Ghent University, Gent, BE

META08_TU Multidimensional HPLC+GC-MS: A Powerful, Novel, Analytical Tool for Universal Quali/Quanti of Volatiles, Semivolatiles and Polar Compounds. Example of Application in Metabolomics. Josep Mª Gibert, NIeves Sarrión, Roger Gibert, David Alonso KONIK-Tech S.A., Sant Cugat del Vallès, ES

META09_WE Towards a LC-MS Library Compatible with Metabolomic Profiling Oskar Gonzalez Mendia, Eric Tijssen, Carola Damen, Rob Vreeken, Thomas Hankemeier Division of Analytical Biosciences, Leiden University, Leiden, NL

META10_MO Molecularly Imprinted Polymers Separation Combined with UHPLC-MS/MS: A Tool for Experimental and Clinical Diagnostics Petr Kacer, Michaela Gajdosova, Kamila Syslova, Marek Kuzma ICT - Prague, Prague 6, CZ

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META11_TU Ion Pair Chromatography vs. HILIC - Survey of Different Separation Techniques for Highly Polar Metabolites Rudolf Köhling, Roland Wohlgemuth, Bernhard Schönenberger Sigma-Aldrich, Buchs SG, CH

META12_WE Rapid Screening for Detection and Differentiation of Adulterated Infant Milk Formula by LC-MS Sam Li NUS, Singapore, SG

META13_MO Metabolite Analysis by LC-Q-TOF-MS to Investigate the Molecular Basis of Storage Disorders in Apple Fruit Tony McGhie1, Daryl Rowan1, Jason Johnston2 1Plant & Food Research, Palmerston North, NZ; 2Plant & Food Research, Havelock North, NZ

META14_TU HPLC-UV Determination of Nonspecific Binding of the Casearin X to Rat Liver Microsomes Rodrigo Moreira Silva1, Anderson R. M. Oliveira1, Alberto J. Cavalheiro2, Norberto P. Lopes1, Cristiane M. Gaitani1 1USP, Ribeirão Preto / SP, BR; 2UNESP, Araraquara / SP, BR

META15_WE Micro Flow LC/MS/MS Applications in Drug Metabolism and Bioanalysis Khaled Mriziq, Remco Van Soest, Tina Settineri Eksigent, part of AB SCIEX, Dublin, CA, US

META16_MO Beer Metabolomics of Light and Dark Beers Robert Murphy Kroungold Analytical, Encinitas, US

META17_TU Simultaneous Analysis of Organic and Inorganic Anions in Saliva Using Anion- Exchange Chromatography–Suppressed Conductivity Detector Yoon Jong Oh1, Ji Eun Kim1, Seung Jin Kim1, Ji Hyun Kim1, Yong Duk Park1, Kyung Suk Hwang2 1Department of Preventive Social Dentistry, Kyung Hee Univ, Seoul, KR; 2Department of Dental Technology, Shinheung Univ., Uijeongbu, Kyunggido, KR

META18_WE Improving Cancer Diagnostics through Assembly of the Cancer Metabolome Jorge Pereira1, Srikanth Rapole2, Hampapathalu Nagarajaram3, David Wishart4, José S. Câmara1 1CQM, UMa, Funchal, PT; 2Scientist Proteomics Lab, National Center for Cell Science (NCCS), Ganeshkhind, IN; 3Laboratory of Computational Biology (LCB), Centre for DNA Fingerprinting and Diagnostics (CDFD), Hyderabad, IN; 4University of Alberta, Department of Computing Science, Edmonton, CA

META19_MO Metabolic Effects of ZnO Nano Particle Exposure on A459 Alveolar Carcinoma Cells Determinded by Reversed Phase LC, HILIC and Gas Chromatography with Mass Spectrometric Detection Roland Reischl1, Christina Ranninger1, Mark Rurik2, Oliver Kohlbacher2, Christian G. Huber1 1University of Salzburg, Salzburg, AT; 2University of Tübingen, Tübingen, DE

META20_TU UHPLC-MS/MS Method for Monitoring of Neurotransmitters and their Metabolites in Brain Microdialysates Kamila Syslova1, Michaela Gajdosova1, Marek Kuzma2, Petr Kacer1 1ICT Prague, Prague, CZ; 2Institute of Microbiology, Prague, CZ

META21_WE Profiling of Plant Hormones Cytokinins by Hyphenated Techniques Petr Tarkowski, Tibor Béres Palacky University, Olomouc, CZ

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META22_MO Hydrophilic Interaction Liquid Chromatography (HILIC) Coupled to Mass Spectrometry (MS) — A Powerful Analytical Platform to Obtain a Broad Metabolites Coverage in Metabolic Profiling Sara Tufi, Marja Lamoree, Pim Leonards Vrije Universiteit, Amsterdam, NL

META23_TU HPLC-MS/MS Flux Measurements for Study of Metabolism in Hsp90 inhibited Cancer Cells Christian Wachsmuth1, Maria Mycielska2, Katja Dettmer1, Sven Lang2, Edward Geissler2, Peter Oefner1 1Institute of Functional Genomics, University of Regensburg, Regensburg, DE; 2Department of Surgery, University Hospital Regensburg, Regensburg, DE

META24_WE Comparison of Pharmacokinetics of Buspirone in Rats At Low Altitude and Acute Exposure High Altitude (Corresponding to an Altitude of 4,100 M) Rong Wang, Juanhong Zhang, Hua Xie, Zhengping Jia, Xiandong meng, Liting Xu, Qiang Zhang, Xiaohui Zhang, Dengfeng Guo Key Laboratory of the plateau of the environmental damage, Lanzhou, CN

META25_MO A Novel LC-MS Approach for Urine Modified Metabolome Profiling Peiyuan Yin, Weidong Dai, Zhongda Zeng, Hongwei Kong, Xin Lu, Guowang Xu Dalian institute of chemical physics, Dalian, CN

META26_TU Development and Optimization of an HPLC-UV Assay for Measurement of Indoleamine2.3-Dioxygenase (IDO) Activity Randa Ghedira Chkir1, Mohamed Hachem Saadaoui2, Wahiba Douki2, Mohamed Fadhel Najjar2, Sallouha Gabbouj1, Abdelfatteh Zakhama1, Lotfi Chouchane1, Elham Hassen1 1Molecular Immuno-Oncology Laboratory, Faculty of Medicin, Monastir, TN; 2Biochemistry and Toxicology Laboratory, University Hospital Fattouma Bourguiba, Monastir, TN

META27_WE Metabolic Profiling of Yeast Arginine Biosynthesis Deletion Mutants Aiko Barsch1, Sandy Yates2, Gabriela Zurek1, Manhong Wu3, Rob van der Heijden4 1Bruker Daltonik GmbH, Bremen, DE; 2Bruker Daltonics, Billerica, MA, US; 3Stanford University, Stanford, CA, US; 4Bruker Daltonics B.V., Wormer, NL

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Monoliths MONO01_MO Selective Monolithic Microemulsion Liquid Chromatographic Method for Simultaneous Quantification of Rosiglitazone and Glimepiride in Wistar Rats Serum and its Application in Pharmacokinetic Studies Mohamed Hefnawy1, Mohammed Al-Omar1, Nasser ALGRAIN1, Sabry Attia2 1Pharmaceutical Chemistry Department,College of Pharmacy, KSU, Riyadh, SA; 2Pharmacology Department,College of Pharmacy, KSU, Riyadh, SA

MONO02_TU Preparation, Characterization and Application of Methacrylate Monolithic Columns for Capillary Liquid Chromatography Ahmad Aqel, Kareem Yusuf, Zeid ALOthman, Yacine Badjah-Hadj-Ahmed King Saud University, Riyadh, SA

MONO03_WE Preparation of Highly Ordered Monolithic Structures by Unidirectional Freezing and Radical Polymerisation R. Dario Arrua, Paul R. Haddad, Emily F. Hilder School of Chemistry, University of Tasmania, Hobart, AU

MONO04_MO Poly(3-Chloro-2-Hydroxypropyl Methacrylate) Based Reactive Monolith with Derivatization Flexibility as a New Separation Medium for Capillary Electrochromatography. Part II: Taurine Functionalized Zwitterionic Stationary Phase Cemil Aydoğan1, Bekir Çelebi2, Adil Denizli1, Ali Tuncel2 1Hacettepe University, Department of Chemistry, Ankara, TR; 2Hacettepe University, Department of Chemical Engineering, Ankara, TR

MONO05_TU Synthesis, Chromatography, and Morphological Analysis of a New TMOS/PTMS Hybrid Capillary Monolith Kristof Hormann1, Romana Hakim2, Bernd Smarsly2, Ulrich Tallarek1 1Philipps-Universität Marburg, Marburg, DE; 2Justus-Liebig-Universität Giessen, Giessen, DE

MONO06_WE Vinyl Phenyl Boronic Acid Functionalized Lauryl Methacrylate Based Monolith for Nano Liquid Chromatography of Small Molecules and Proteins Cemil Aydoğan1, Fatma Yılmaz2, Adil Denizli1 1Hacettepe University, Department of Chemistry, Ankara, TR; 2Abant Izzet Baysal University, Gerede Vocational School, Bolu, TR

MONO07_MO Monolithic Stationary Phases for Capillary Liquid Chromatography Based on Silica and Zirconium Oxides Carla Grazieli Azevedo da Silva, Raquel Costa Silva, Carol Collins, Carla Beatriz Grespan Bottoli University of Campinas (UNICAMP), Campinas/SP, BR

MONO08_TU Miniaturized Immunosorbents Coupled On-Line with Nanolc : Synthesis, Characterization and Applications to Environmental Samples Fabien Brothier, Valérie Pichon LSABM - UMR PECSA 7195 ESPCI ParisTech, PARIS, FR

MONO09_WE Silica-based Monoliths and Particulate Beds: A Morphological Comparison Stefan Bruns, Alexandra Höltzel, Ulrich Tallarek Philipps-Universität Marburg, Marburg, DE

MONO10_MO Detailed Characterization of the Kinetic Performance of First and Second Generation Monolithic Silica Columns Deirdre Cabooter1, Roman Sterken1, Alison Vanmessen1, Sander Deridder2, Gert Desmet2 1K.U.Leuven, Leuven, BE; 2V.U.B., Brussel, BE

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MONO11_TU Determination of Parabens in Human Urine and Serum by Capillary Liquid Chromatography Using Methacrylate Monolithic Columns Enrique Javier Carrasco Correa1, Fernando Vela-Soria2, Oscar Ballesteros2, José Manuel Herrero- Martínez1 1Dept. de Química Analítica, Universidad de Valencia, Burjassot, Valencia, ES; 2Dept. de Química Analítica, Universidad de Granada, Granada, ES

MONO12_WE Agilent Bio-Monolith Protein A column – Monitoring of monoclonal antibody titer and yield from supernatants Andrew Coffey1, Phu Duong2, Maureen Joseph2 1Agilent Technologies UK Ltd, Church Stretton, GB; 2Agilent Technologies Inc, Wilmington, US

MONO13_MO Morphological Similarity and Radial Homogeneity of Silica Monoliths Kristof Hormann, Dzmitry Hlushkou, Ulrich Tallarek Philipps-Universität Marburg, Marburg, DE

MONO14_TU Performance Characterization of Polymer Monolithic Columns for Small-Molecule Separations Using Total-Pore-Blocking Conditions Catherine Stassen, Gert Desmet, Sebastiaan Eeltink Vrije Universiteit Brussel, Brussels, BE

MONO15_WE Photografting as a Simple and Versatile Functionalization Route of Silica-Based Monoliths Racha El Debs, Claire Demesmay, Vincent Dugas Institute of Analytical Sciences, Villeurbanne, FR

MONO16_MO New Polar Coatings for Stir Bar Sorptive Extraction of Emerging Pollutants Núria Fontanals1, Núria Gilart1, Núria Miralles1, Peter A. G. Cormack2, Francesc Borrull1, Rosa M. Marcé1 1Universitat Rovira i Virgili, Tarragona, ES; 2University of Strathclyde, Glasgow, GB

MONO17_TU Polymer Monolith Microextraction with UPLC-UV for the Determination of Phenylurea Herbicides in River Water Ming-Ren Fuh, Yu-Ru Wu, Shu-Ling Lin Soochow University, Taipei, TW

MONO18_WE Comparison of First and Second Generation Analytical Silica Monoliths by Pore-Scale Simulations of Eddy Dispersion in the Bulk Region Dzmitry Hlushkou1, Kristof Hormann1, Alexandra Höltzel1, Siarhei Khirevich1, Andreas Seidel- Morgenstern2, Ulrich Tallarek1 1Philipps-Universität Marburg, Marburg, DE; 2Max-Planck-Institut für Dynamik Komplexer Technischer Systeme, Marburg, DE

MONO19_MO Fast Analysis of Phthalates and Degradation Products in Waters Using In-Tube Solid Phase Microextraction Coupled On-Line to Capillary Liquid Chromatography Neus Jornet-Martinez, Maria Muñoz-Ortuño, Yolanda Moliner-Martinez, Rosa Herráez- Hernández, Jorge Verdú-Andrés, Pilar Campíns-Falcó Universidad Valencia, Burjassot, ES

MONO20_TU Poly(3-Chloro-2-Hydroxypropyl Methacrylate) Based Reactive Monolith with Derivatization Flexibility as a New Separation Medium for Capillary Electrochromatography. Part III: Polyethylenimine Functionalized Strong Anion Exchange Stationary Phase Cemil Aydoğan1, Bekir Çelebi2, Aslıhan Gökaltun2, Adil Denizli1, Ali Tuncel2 1Hacettepe University, Department of Chemistry, Ankara, TR; 2Hacettepe University, Department of Chemical Engineering, Ankara, TR

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MONO21_WE Monolithic Poly (N-vinlycarbazole-co-1,4-divinylbenzene) Stationary Phases Optimized for the Separation of Biomolecules or Small Molecules Rainer Koeck1, Martin Fischnaller1, Rania Bakry1, Richard Tessadri2, Guenther K. Bonn1 1Analytical Chemistry and Radiochemistry, Innsbruck, AT; 2Mineralogy and Petrography, Innsbruck, AT

MONO22_MO Modelling the B-Term Band Broadening in Monolithic Silica Columns Sander Deridder1, Deirdre Cabooter2, Alison Vanmessen2, Gert Desmet1 1Vrije Universiteit Brussel, Elsene, BE; 2K.U. Leuven, Leuven, BE

MONO23_TU Incorporation of Sporopollenin Particles Into Polymethacrylate Based Monolithic Capillary Columns to Be Use as Templates for HPLC Separations Kareem Yusuf1, Ahmad Aqel1, Amro Dyab2, Zeid ALOthman1, Yacine Badjah-Hadj-Ahmed1 1Advanced Materials Research Chair- King Saud University, Riyadh, SA; 2Collage of Science- King Saud University, Riyadh, SA

MONO24_WE Comparison of Performance of Monolithic Silica Columns Clad by Glass or Stainless Steel Riichi Miyamoto1, Yukiko Ando2, Chie Kurusu2, Hong-zhi Bai2, Kazuki Nakanishi1, Masamichi Ippommatsu2 1Graduate School of Science, Kyoto University, Kyoto, JP; 2Renaissance Energy Investment, Co., Ltd., Kyoto, JP

MONO25_MO Reconstruction and Characterization of Polymeric Monolithic Stationary Phases Using Serial Block Face Scanning Electron Microscopy Tibor Muellner1, Armin Zankel2, Herbert Reingruber2, Ulrich Tallarek1 1Philipps-Universität Marburg, Marburg, DE; 2Institute for Electron Microscopy, Technische Universität Graz, and Center for Electron Microscopy Graz, Graz, AT

MONO26_TU Development of Hypercrosslinked Monolithic Stationary Phases for Chiral Capillary Liquid Chromatography Veronika Skerikova, Kristyna Dedova, Jiri Urban University of Pardubice, Pardubice, CZ

MONO27_WE 3D Reconstruction of a Mesoporous Monolithic Silica Structure Using Scanning Transmission Electron Microscopy (STEM) Tomography Daniela Stoeckel1, Christian Kübel2, Ulrich Tallarek3, Bernd Smarsly1 1Justus-Liebig-University, Giessen, DE; 2Karlsruhe Institute of Technology, Karlsruhe, DE; 3Philipps-Universita, Marburg, DE

MONO28_MO Comparison of Chromolith Performance RP-18e, 1.8μm Zorbax Eclipse XDB-C18 and XTerra MS C18 Columns in the Analysis of Phenols and β-Adrenolytic Drugs José Ramón Torres-Lapasió1, Sandra Pous-Torres2, María Celia García-Álvarez-Coque1 1University of Valencia, Burjassot (Valencia), ES; 2University of Amsterdam, Amsterdam, NL

MONO29_TU Hypercrosslinked Monolithic Capillary Columns for Reversed-Phase and Hydrophilic Interaction Liquid Chromatography of Small Molecules Jiri Urban, Veronika Skerikova, Pavel Jandera University of Pardubice, Pardubice, CZ

MONO30_WE Comparison of Various Silica-Based Monoliths for the Analysis of Large Biomolecules Karine Vuignier, Szabolcs Fekete, Pierre-Alain Carrupt, Jean-Luc Veuthey, Davy Guillarme University of Geneva, Switzerland, Geneva 4, CH

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MONO31_MO Titanium-Reinforced Polymer-Based Monolithic Stationary Phases for Application in Liquid Chromatography R.J. Vonk1, S. Eeltink2, P.J. Schoenmakers1 1Univeristeit van Amsterdam, Amsterdam, NL; 2Vrije Univeristeit Brussel, Brussel, BE

MONO32_TU Thermally Polymerized Organo-Silica Monoliths: Optimization of Preparation Conditions and Pore Surface Functionalization Zuzana Zajickova1, Anna-Marie Weed1, Deepa Gharbharan1, Gabriela Soto1, Frantisek Svec2 1Barry University, Miami Shores, FL, US; 2The Molecular Foundry, Berkeley, CA, US

MONO33_WE Characterization of Polymer Monolithic Stationary Phases Using Differential Scanning Calorimetric Techniques Sam Wouters, Bert Wouters, Axel Vaast, Guy Van Assche, Sebastiaan Eeltink Vrije Universiteit Brussel, Brussel, BE

MONO34_MO Effect of Crosslinking Monomers on the Efficiency of Monolithic Capillary Columns for Hydrophilic Interaction Chromatography Magda Staňková, Pavel Jandera, Jiří Urban, Veronika Škeříková University of Pardubice, Pardubice, CZ

MONO35_TU Nanoscale Characterization of Polymer Monoliths Using Atomic Force Microscopy and Confocal Raman Imaging Martin Laher1, Tim J. Causon2, Wolfgang Buchberger2, Sabine Hild1, Ivo Nischang3 1Institute of Polymer Science/JKU Linz, Linz, AT; 2Institute of Analytical Chemistry/JKU Linz, Linz, AT; 3Institute of Polymer Chemistry/JKU Linz, Linz, AT

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MS Data Analysis MSDA01_MO Determination of Emerging Illicit Drugs in Waste Water María Jesús Andrés-Costa, Ana Masiá, Yolanda Picó University of Valencia, Valencia, ES

MSDA02_TU Meeting CODEX Guidelines: Utilisation of Complete Workflow Procedures for Pesticide Residue Analysis Antonietta Gledhill1, Sara Stead1, Mike McCullagh1, Dominic Roberts1, Richard Fussell2 1Waters Corporation, Manchester, GB; 2FERA, York, GB

MSDA03_WE LC-QTOFMS Analysis of Polyethylene Glycol (PEG) and Pegylated Lysozyme Ravindra Gudihal1, Sundaram M Palaniswamy1, Lakshmi NS2, Ning Tang3 1Agilent Technologies India Pvt. Ltd, Bangalore, Karnataka, IN; 2Amrita University, Kollam, Kerala, IN; 3Agilent Technologies Inc, Santa Clara, CA, US

MSDA04_MO Suitable Gradient Conditions for the Separation of Bio-Oils Samples in RPLC X RPLC Sabine Heinisch1, Cécile Gouin2, Agnès LeMasle2, Jérémie Ponthus2, Alain Quignard2 1Institut des Sciences Analytiques, Villeurbanne, FR; 2IFPEN, Solaize, FR

MSDA05_TU Feature Detection in LC-MS Fingerprints Using the Combined Matched Gaussian Filter and Two-Fold Differentiation with Median Measures for Threshold Determination (Comfidimed) Nikoline J. Nielsen1, Rolf Danielsson2, Jan H. Christensen1 1University of Copenhagen, Frederiksberg C, DK; 2Uppsala University, Uppsala, SE

MSDA06_WE Comprehensive Chemical Fingerprinting of Chamomile SFE Extracts Using UPC2/MS Michael Jones1, Giorgis Isaac2, Bharathi Avula3, Yan-Hong Wang3, Kate Yu4, Troy Smillie3, Iklas Khan5, Norman Smith1 1King's College, London, London, GB; 2Waters Corp., Milford, MA, US; 3NCNPR, University, MS, US; 4Waters Corp, Milford, US; 5University of Mississippi, University, MS, US

MSDA07_MO Quality of Ultrapure Water for Environmental Analyses of Hormones and Pesticides in Water Samples at Ng/L Levels Anastasia Khvataeva-Domanov1, Mikael Tilli2, Jevgeni Parshintsev2, Matias Kopperi2, Marja-Liisa Riekkola2, Cecilia Devaux1, Patrik Appelblad3, Stephane Mabic1 1Merck Millipore, Guyancourt, FR; 2University of Helsinki, Helsinki, FI; 3Merck Millipore, Umea, SE

MSDA09_WE Data Processing Method Development for HPLC-MS-Based Metabonomics Xiaohui Lin, Hai Wei, Jiuchong Gao, Xiangfei Ding Dalian University of Technology, Dalian, CN

MSDA10_MO Evaluation of Photodegradation Profile of Lercanidipine Enantiomers by HPLC-UV and Characterization of Degradation Products by LC-ESI/MS-MS Luciana Lourenço, Cristiane Gaitani, Luiz Moraes University of São Paulo, Ribeirão Preto - SP, BR

MSDA11_TU LCxLC-MS Separations of Protein Tryptic Digests Sabine Heinisch, Amélie D Attoma, Marion Iguiniz Institut des Sciences Analytiques, Villeurbanne, FR

MSDA12_WE Matching Features across Chromatographic Platforms to Assess Orthogonality for Liquid Chromatography Mass Spectrometry Fingerprinting Nikoline J. Nielsen1, Giorgio Tomasi2, Jan H. Christensen1 1University of Copenhagen, Frederiksberg C, DK; 2European Commission Joint Research Centre, Ispra, IT

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MSDA13_MO Development, Optimization and Validation of a Fingerprinting Method for Simultaneously Determining Guaianolide and Parthenolide in Tanacetum Parthenium (L.) Schultz-Bip Using Quality-By-Design Approach and Liquid Chromatography Mirela F. Rabito1, Mariana B. Almeida2, Amadeu H. Iglesias3, Amanda L. Moreira1, Fernando De Paula3, Izabel C. P. Ferreira1, Suzana Lucy Nixdorf2 1Universidade Estadual de Maringá, Maringá, BR; 2Universidade Estadual de Londrina, Londrina, BR; 3Waters Technology do Brasil, Sao Paulo, BR

MSDA14_TU Metabolomics of Urinary Tract Infection Tiziana Pacchiarotta, Rico Derks, Katja Nevedomskaya, Paul Hensbergen, Manfred Wuhrer, Willize van der Starre, Bart Schoenmaker, Jaap van Dissel, André Deelder, Oleg Mayboroda LUMC, Leiden, NL

MSDA15_WE Identification of Unknowns Using High Resolution Liquid Chromatography/Mass Spectrometry (LC/MS), LC/MS/MS, Liquid Chromatography/Infrared Spectroscopy (LC/IR), and Gas Chromatography/Mass Spectroscopy (GC/MS) Teresa Peterson1, Rick Borders1, Wade Thompson1, Fred Stroebel2, Tracy Phillpott3 1Kimberly-Clark Corporation, Roswell, Georgia, US; 2Emory University, Atlanta, Georgia, US; 3Spectra Analysis, Greenville, South Carolina, US

MSDA16_MO Pesticides in Source and Drinking Waters in the State of São Paulo (Brazil) by SPE-LC- ESI-MS/MS Cassiana Raimundo1, Cristiane Vidal2, Raphael Acayaba1, Gisela Umbuzeiro1, Isabel Jardim2 1University of Campinas, Limeira, BR; 2University of Campinas, Campinas, BR

MSDA17_TU In Vitro Metabolism of Monensin A: Identification of Metabolites by LC-MS/MS Spectrometry Bruno Rocha, Marilda Assis, Ana Peti, Luiz Moraes, Norberto Lopes, Anderson de Oliveira University of São Paulo, Ribeirão Preto, SP, BR

MSDA18_WE Integrity Assessment of Thymopentin under Conditions for Production of Hydrogels by LC–MSn Mehrnoosh Shirangi, Javier Sastre Toraño, Wim E Hennink, Govert W Somsen, Cornelus F van Nostrum Utrecht University, Utrecht, NL

MSDA19_MO Fast Multi-Residue Method for Determination of Nineteen Benzimidazoles in Meat by Liquid Chromatography and Tandem Mass Spectrometry Georgi Stoev, Milena Peicheva, Alexander Stoyanov CLVCE, Sofia, BG

MSDA20_TU HPLC and LC-MS/MS for the Detection of Early Biomarkers of Neonatal Growth in Human Amniotic Fluid Christina Virgiliou, Maria Fotiou, Alexandra Michailidou, George Theodoridis, Apostolos Athanasiadis, George Menexes, Vasiliki Koulourida, Costas Biliaderis, Vasilios Tarlatzis Aristotle University of Thesaloniki, Thesaloniki, Gr

MSDA21_WE Batch Effects in Liquid Chromatography: Setting the Data Straight Franziska Vogl, Jochen Hochrein, Wolfram Gronwald, Katja Dettmer-Wilde, Peter Oefner Institute of Functional Genomics, Regensburg, DE

MSDA22_MO Characterization of Commercially Available Stevia-Derived Sweeteners Using Liquid Chromatography-High Resolution Time of Flight Mass Spectrometry and Advanced Data Processing Juergen Wendt1, Jutta Lintelmann2 1LECO European LSCA Centre, Moenchengladbach, DE; 2Helmholtz Zentrum München, Neuherberg, DE

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MSDA23_TU Urinary Biomarker for Behce'ts Disease Using LC/ MS-MS and HPLC-DAD Saied Jaradat1, Khaldoon Alawneh2, Mohammad Dwairi1 1Jordan university of Science and Technology, Irbid, JO; 2King Abdullah University Hospital, Irbid, JO

MSDA24_WE Validation of a Method for the Determination of Thyreostatic Drugs in Urine by LC- MS/MS Elmas Öktem Olgun, Öznur Karaoğlu, Muammer Kaplan Tubitak Mrc Food Institute, Kocaeli, TR

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Nanomaterials NANO01_MO Measurement of Quercetin by High-Performance Liquid Chromatography Coupled to Photodiode Array Detection for Pharmacokinetic Application of Ultrasound- Triggered Micro-Formulation Li-Wen Chang, Mei-Ling Hou, Tung-Hu Tsai Institute of Traditional Medicine, NYMU, Taipei, TW

NANO02_TU Preparation and Evaluation of Methacrylate Monoliths Functionalized with Silver Nanoparticles Marta Mon-Conejero, María Navarro-Pascual-Ahuir, Ernesto Simó-Alfonso, Guillermo Ramis- Ramos, José Manuel Herrero-Martínez Dept. Analytical Chemistry, University of Valencia, Burjassot (Valencia), ES

NANO03_WE Immobilization of PNGASE F onto Gold Nanoparticles via Linker SH-PEG7-COOH. Markus Höldrich, Michael Lämmerhofer University of Tübingen, Institute of Pharmaceutical Science, Tübingen, DE

NANO04_MO Application of Miniaturized Sample Preparation Technique: -Hydroxyprene Case Study Okechukwu Ifegwu1, Chimezie Anyakora1, Nelson Torto2 1University of Lagos, Akoka, Lagos, Nigeria, Lagos, Ng; 2rhodes University, Grahamstown, Za

NANO05_TU The Use of Nanometer-Scale Chemicals to Make New Separation Media Showing Unique Selectivity and Stability Xiaodong Liu, Christopher Pohl Thermo Fisher Scientific, Sunnyvale, California, US

NANO06_WE Optical Isomer Separation of Flavanones and Flavanone Glycosides by Nano-Liquid Chromatography Using a Phenyl-Carbamate-Propyl-B-Cyclodextrin Chiral Stationary Phase Fairouz Tazerouti1, Kahina Si-Ahmed1, Ahmed Yacine , Badjah-Hadj-Ahmed1, Zeineb Aturki2, Giovanni D’Orazio2, Anna Rocco2, Salvatore Fanali2 1University of Science and Technology Houari Boumedien, USTHB, Algiers, DZ, 2Institute of Chemical Methodologies, Italian National Council of Research, Area della Ricerca di Roma, Rome, IT

NANO07_MO Electrospun Nanofiber Based Sorbents for Sample Clean-Up of Environmental Or Biological Samples Nelson Torto, Samuel Chigome, Boitumelo Mudabuka, Bellah Pule, Dezzline Ondigo, Zenixole Tshentu Rhodes University, Grahamstown, ZA

NANO08_TU High-Speed Gradient Separations of Peptides and Proteins Using Polymer-Monolithic Poly(Styrene-Co-Divinylbenzene) Capillary Columns at Ultra-High Pressure Axel Vaast1, Lucie Nováková1, Bjorn de Haan2, Remco Swart2, Sebastiaan Eeltink1 1Vrije Universiteit Brussel, Brussels, BE; 2Thermo Fisher Scientific, Amsterdam, NL

NANO09_WE Magnetic Nanoparticles Coated with Immobilized Alkaline Phosphatase for Enzyme Inhibition Assays by HPLC Siming Wang, Ping Su, Jun Huang, Jingwei Wu, Yi Yang Beijing University of Chemical Technology, Beijing, CN

NANO10_MO Highly Efficient Extraction of the Cellular Nucleic Acid Associated Protein in Vitro with Magnetic Oxidized Carbon Nanotube Yi Zhang, Zhengyan Hu, Hongqiang Qin, Xiaoluan Wei, Kai Cheng, Fangjie Liu, Ren'an Wu, Hanfa Zou Dalian Institute of Chemical Physics, CAS, Dalian, CN

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NANO11_TU Highly Sensitive Determination of Phenylarsonic Acids Based on Sorptive Extraction of Carboxymethyl A- Cyclodextrin Immobilized Fe3O4 Magnetic Nanoparticles by High Performance Liquid Chromatography-Mass/Mass Spectrometry Ruohua Zhu1, Peilong Wang2, JIng Jia1 1 2 Capital Normal University, Beijing, CN; Institute of Quality Standards and Testing Technology for Agriculture Products, Beijing, CN

NANO12_WE Synthesis of Silver Nanoparticles Confined in Hierarchically Porous Monolithic Silica: A New Function in Aromatic Hydrocarbon Separations Yang Zhu1, Kei Morisato2, Wenyan Li3, Kazuyoshi Kanamori1, Kazuki Nakanishi1 1Kyoto University, Kyoto, JP; 2GL Science, Inc., Tokyo, JP; 3Zhejiang University, Hangzhou, CN

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Pharmaceutical PHAR01_MO Assay Develoment and Method Validation of Colchicine Tablets by RP-HPLC Arman Abrahamyan Arpimed LLC, Abovyan, AM

PHAR02_TU HPLC Method for Analysis of Prochelator – Boronyl Salicylaldehyde Isonicotinoyl Hydrazone and its Application to in Vitro Activation Study Jan Bures, Hana Jansova, Petra Kovarikova Faculty of Pharmacy, Charles University, Hradec Kralove, CZ

PHAR03_WE Response Surface Methodology in Optimization of HPLC Analysis of Fluoxetine and Olanzapine Fatih Karademir, Mehmet Gokhan Caglayan, İsmail Murat Palabiyik, Feyyaz Onur Ankara University, Ankara, TR

PHAR04_MO Development of an HPLC Method for Determination of Related Impurities in Iodixanol Drug Substance Neila Cassiano1, Bruno Rondon2, Quezia Cass1 1Federal University of São Carlos, São Carlos - São Paulo, BR; 2U.S. Pharmacopeia of Brazil, Barueri - São Paulo, BR

PHAR05_TU Predicting Drug Penetration Across the Blood-Brain Barrier: Comparison of Micellar Liquid Chromatography and Immobilized Artificial Membrane Liquid Chromatography Mike De Vrieze1, Frédéric Lynen1, Kai Chen1, Roman Szucs2, Pat Sandra1 1Ghent University, Ghent, BE; 2Pfizer, Sandwich, GB

PHAR06_WE Pharmacokinetic Studies of Loosening the Isoniazid Aleš Eisner, Adam Martin, Bajerová Petra, Bajer Tomáš, Ribárová Pavla, Ventura Karel Univerzity of Pardubice, Pardubice, CZ

PHAR07_MO Stability-Indicating UPLC Assay Method for Moxonidine Active Pharmaceutical Ingredient and Pharmaceutical Dosage Form Jelena Golubovic1, Svetlana Milovanovic2, Biljana Otasevic1, Mira Zecevic1, Ana Protic1 1University of Belgrade-Faculty of Pharmacy, Belgrade, RS; 2Medicines and Medical Devices Agency of Serbia, Belgrade, RS PHAR08_TU Simultaneous Analysis of Gadolinium Chelates as Ionic MRI Contrast Agents and Anions by High Performance Ion Chromatography Peter Hajos, Diana Lukacs, Flora Geczo, Krisztian Horvath University of Pannonia, Veszprem, HU

PHAR09_WE Head and Neck Irradiation Modulated Both Pharmacokinetics of 5-Fluorouracil and Cisplatin in Rats Chen-Hsi Hsieh1, May-Ling Hou2, Hung-Chi Tai3, Hui-Ju Tien1, Li-Ying Wang4, Yu-Jen Chen3, Tung-Hu Tsai2 1Far Eastern Memorial Hospital, New Taipei City, TW; 2National Yang-Ming University, Taipei, TW; 3Mackay Memorial Hospital, Taipei, TW; 4National Taiwan University, Taipei, TW

PHAR10_MO Research of Pyridinium Compounds in Plants with Therapeutic Properties – UPLC/MS Studies Malgorzata Jakubowska, Malgorzata Urbaniak, Andrzej Marcinek, Jerzy Gebicki Lodz University of Technology, Lodz, PL

PHAR11_TU Determination of Amlodipine Besylate in Pharmaceutical Preparations by Green Chromatographic Method Juhee Jung, Junghyun Kim, Unyong Kim, Joon Hyuk Suh, Han Young Eom, Hyun-Deok Cho, Yura Jung, Jeong-Rok Youm, Sang Beom Han Chung-Ang University, Seoul, KR

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PHAR12_WE Sphingosine 1-Phosphate Lyase Activity Measurement by HPLC-Fluorescence Detection Yong-Moon Lee, Kyong-Oh Shin, Cho-Hee Seo, Shin-Hee Lee College of Pharmacy, Chungbuk National University, Chongju, KR

PHAR13_MO Streamlining Analysis of Desogestrel and Ethinyl Estradiol Oral Contraceptives with UPLC Margaret Maziarz, Sean M. McCarthy, Micheal D. Jones, Warren B. Potts Waters Corporation, Milford, MA, US

PHAR14_TU Development and Validation of Rapid UHPLC Method for Determination of Risperidone and its Impurities in Bulk Powder and Tablets. Tomas Nejedly, Pavla Pilarova, Petr Kastner, Jiri Klimes Charles University in Prague, Faculty of Pharmacy, Hradec Kralove, CZ

PHAR15_WE HPLC Method for Quantifying Tenofovir Disoproxil Fumarate in Raw Material, Tablet and their Degradation Products Mariana B. Almeida, Mirela F. Rabito, Erieldes S. Silva, Vinicius Acquaro Junior, Tiago B. Madeira, Marlene M. F. Nery, Suzana Lucy Nixdorf Universidade Estadual de Londrina, Londrina, BR

PHAR16_MO The HPLC Method for Simultaneous Determination of Meropenem and Clavulanate Potassium Magdalena Paczkowska, Przemyslaw Zalewski, Judyta Cielecka-Piontek Dep. of Pharm. Chemistry, Poznan University of Medical Sciences, Poznan, PL

PHAR17_TU Antiarrhytmics Determination by Liquid Chromatography Juan Peris-Vicente1, Samuel Carda-Broch1, Beatriz Beltran-Martinavarro1, Maria Rambla- Alegre1, Enrique Ochoa-Aranda2, Josep Esteve-Romero1 1University Jaume I, Castelló, ES; 2Hospital Provincial, Castelló, ES

PHAR18_WE Determination of Flavones in Passiflorae Herba (Passiflora Incarnata L., Passifloraceae) by UHPLC Klaus Reif1, Silvia Sponza2, Johannes Novak2, Hartwig Sievers1 1PhytoLab GmbH & Co. KG, Vestenbergsgreuth, DE; 2University of Veterinary Medicine Vienna, Vienna, AT

PHAR19_MO Ion Chromatography: An Important Technique for Assay and Analysis of Drug Substances and Drug Products Jeffrey Rohrer1, Lipika Basumallick1, Suparerk Tukkeeree2 1Thermo Fisher Scientific, Sunnyvale, CA, US; 2Thermo Fisher Scientific, Bangkok, TH

PHAR20_TU Stability Indicating LC Method and LC-Qtofms Studies for the Separation and Identification of Degradation Products of Darunavir Josilene Corrêa1, Cristina Serra2, Guilherme Titato3, Fernando Lanças3, Alvaro Santos-Neto3, Hérida Salgado1 1School of Pharmaceutical Sciences / UNESP, Araraquara, SP, BR; 2School of Pharmaceutical Sciences / University of São Paulo, São Paulo, SP, BR; 3Institute of Chemistry of São Carlos / University of São Paulo, São Carlos, SP, BR

PHAR21_WE A Risk- and Science-Based Quality-By-Design Approach in UPLC Method Development – Case Study Ebastine Alexander H. Schmidt, Mijo Stanic Steiner Pharmaceuticals, Berlin, DE

PHAR22_MO Highres MS Screening of Known and Unknown Impurities and Related Substances in Stability Study of Selected Pharmaceuticals. Ludovit Schreiber, Radoslav Halko, Milan Hutta Faculty of Natural Sciences Comenius University in Bratisl, Bratislava, SK 120

PHAR23_TU The HPLC-DAD Method for Stability Studies of Tebipenem Pivoxyl Magdalena Paczkowska, Judyta Cielecka-Piontek, Przemyslaw Zalewski Dep.of Pharm.Chemistry,Poznan University of Medical Sciences, Poznan, PL

PHAR24_WE HPLC-MS Methods for Detection of Pt(II) and Pt(IV) Complexes with Large Lipophilic Ligands Kamila Syslova, Petra Rehackova, Jan Svoboda, Milos Mikoska, Petr Kacer ICT Prague, Prague, CZ

PHAR25_MO An Alternative Approach for the Estimation of Variance Components of Analytical Results in the Pharmaceutical Industry Zsolt Szemők1, Katalin Ganzler1, Sándor Kemény2 1Gedeon Richter Plc., Budapest, HU; 2Budapest University of Technology and Economics, Budapest, HU

PHAR26_TU Development of a Novel and Rapid Isocratic Rp-HPLC Method for the Analysis of Peptide Drug (Salmon Calcitonin)in Pharmaceutical Formulations Cetin Tas1, Saffar Mansoor2, Ajay K. Banga3, Mark R. Prausnitz4 1Gulhane Military Medical Academy Dept. of Pharm. Sci., Ankara, TR; 2School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, US; 3Department of Pharmaceutical Sciences College of Pharmacy and Health Sciences Mercer University, Atlanta, US; 4School of Chemical and Biomolecular Engineering Georgia Institute of Technology, Atlanta, US

PHAR27_WE UPLC-FLR Study for Determination of Nitric Oxide Production from Potential Nitric Oxide Donors Malgorzata Urbaniak, Adam Sikora, Andrzej Marcinek, Jerzy Gebicki Lodz University of Technology, Lodz, PL

PHAR28_MO A High Resolution MS, MS/MS and UV Database of Fungal Secondary Metabolites as a Dereplication Protocol for Bioactive Natural Products Albert Van Wyk1, Tamam El-Elimat2, Mario Figueroa2, Brandie Ehrmann2, Nadja Cech2, Cedric Pearce2, Nicholas Oberlies2 1Advanced Chemistry Development (ACD/Labs), Bracknell, GB; 2Dept. of Chemistry and Biochemistry, University of North Carolina, Greensboro, US

PHAR29_TU Development of Gradient HPLC Method for Simultaneous Determination of Sotalol Hydrochloride and Potassium Sorbate in Oral Liquid Paediatric Preparations Lukas Zahalka1, Oxana Zahalkova1, Ludmila Matysova1, Sylva Klovrzova2, Zdenka Sklubalova1, Petr Solich1 1Charles University in Prague, Faculty of Pharmacy, Hradec Kralove, CZ; 2General Hospital in Motol, Hospital Pharmacy, Prague, CZ

PHAR30_WE Stability-Indicating HPLC Method for the Determination of Cefozopran Hydrochloride Magdalena Paczkowska1, Przemyslaw Zalewski1, Judyta Cielecka-Piontek1, Anna Krause2 1Dep.of Pharm.Chemistry,Poznan University of Medical Sciences, Poznan, PL; 2PozLab, Contract Research Organization, Poznan, PL

PHAR31_MO Use of a Multidimensional Design Space for in Silico Robustness Testing of a Compendial LC Purity Method Alexander H. Schmidt, Mijo Stanic Steiner Pharmaceuticals, Berlin, DE

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PHAR32_TU Determination of Trioxsalen in Capsules by High Performance Liquid Chromatography Naiana Gondim Pereira Barros Lima1, Igor Prado Barros Lima1, Denise Michele Costa Barros1, Thays Santos Oliveira1, Karla Danielle Gomes dos Santos1, Lilian Grace Silva Solon1, Cicero Flavio Soares Aragao1, Ana Claudia Dantas de Medeiros2 1UFRN, Natal, BR; 2UFCG, Campina Grande, BR

PHAR33_WE Transfer of USP Methods for Impurities Analysis of Ziprasidone Hcl between HPLC Systems and to UPLC Margaret Maziarz, Michael D. Jones, Frederic Forini, Warren B. Potts, Sean M. McCarthy Waters Corporation, Milford, MA, US

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Polymers POLY01_MO Advances in Size-Based Separations of Synthetic Polymers Bonnie Alden, Ed Bouvier, Ken Glose, Pamela Iraneta, Gary Izzo, Nicole Lawrence, Mike Savaria, Jeremy Shia, Mia Summers, Tom Walter, Jessica Wilson, Kevin Wyndham Waters Corporation, Milford MA, US

POLY02_TU A Novel Selective Molecularly Imprinted Polymer for Application in Solid Phase Extraction for the Determination of Hydrochlorothiazide in Urine Using HPLC Leonardo Barros, Susanne Rath University of Campinas, Campinas/SP, BR

POLY03_WE Monitoring the Degradation of Stabilizers for Polypropylene after Exposure to Heat by HPLC-MS/MS Susanne Beißmann1, Wolfgang Buchberger1, Gernot Wallner2 1JKU - Institute of Analytical Chemistry (JKU-IAC), Linz, AT; 2JKU - Institute of Polymeric Materials and Testing (JKU-IPMT), Linz, AT

POLY04_MO Size Exclusion Chromatography as an Alternative Technique for the True Boiling Point (TBP) and Final Boiling Point (FBP) Determination of High Boiling Petroleum Fractions and Products Boczkaj Grzegorz, Marian Kamiński Gdansk University of Technology, Gdansk, PL

POLY05_TU Using the Hydrophobic Subtraction Model to Choose Orthogonal Columns for Online Comprehensive Two-Dimensional Liquid Chromatography Rune Græsbøll, Nikoline Nielsen, Jan Christensen University of Copenhagen, Frederiksberg C, DK

POLY06_WE High Temperature Gradient Liquid Chromatography of Bimodal High Density Polyethylene Kavimani Nagar Prabhu1, Tibor Macko1, Robert Brüll1, Priya Garg2, Linda Havermans2, Klaas Remerie2 1Fraunhofer Institute, LBF, Darmstadt, DE; 2SABIC, Geelen, NL

POLY07_MO Preparation and Characterization of Mesoporous Organosilica Adsorbents Xue Min, Xu Zhong, Tai Yang, Zihui Meng Beijing Insititute of Technology, Beijing, CN

POLY08_TU Analysis of Purified Terephthalic Acid (PTA) by Capillary Zone Electrophoresis John C. Hudson1, Jim Thorn1, Hans Dewald1 , Zhen-lei Peng2 1 Beckman Coulter Inc., Brea, CA, US; 2 Sinopec SRIPT, Shanghai, CN

POLY09_WE A Screen for Eleven Electrospun Nanofiber Sorbents for their Sorption Capacity towards a Carcinogenic Biomarker: 1-Hydroxypyrene Okechukwu Ifegwu1, Samuel Chigome2, Chimezie Anyakora1, Nelson Torto2 1University of Lagos, Akoka, Lagos, NG; 2Rhodes University, Grahamstown, ZA

POLY10_MO Application of Electrophoretically Mediated Microanalysis to the In-Capillary Digestion of Poly-L-Lysine Amal Ibrahim, Andrea Megret, Gaëlle Coussot, Catherine Perrin Institute of Biomolecules Max Mousseron, UMR 5247 CNRS, UM, Montpellier, FR

POLY11_TU Using CE-MS for the Identification of Polyimide Materials Christian Klampfl, Timothy Causon, Markus Himmelsbach, Wolfgang Buchberger Johannes Kepler University Linz, Linz, AT

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POLY12_WE Preparation and Characterization of Ethinyloestradiol Molecular Imprinted Membrane Xue Min, Xiaofeng Wu, Xiangrong Mu, Zihui Meng Beijing Institute of Technology, Beijing, CN

POLY13_MO Analysis of Stabilisers in Plastic Materials Used for Photovoltaic Modules by HPLC and MS Techniques Ingrid Hintersteiner1, Wolfgang Buchberger1, Gernot Wallner2 1Institut of Analytical chemistry, JKU Linz, Linz, AT; 2Institut of Polymer and Material Testing, JKU Linz, Linz, AT

POLY14_TU Advances in SEC for Polymer Engineering Applications Michael OLeary, Bonnie Alden, Edouard Bouvier, Peter Hancock, Jeremy Shai, Mia; ummers, Marian Twohig Waters Corp, Milford, MA, US

POLY15_WE Evaluation of Electrospun/Electrospray Polysulfone Fibers and Particles as SPE Sorbent for Pesticides Analysis Noor Gulamussen, Nelson Torto Rhodes University, Grahamstown, ZA

POLY16_MO Gradient Liquid Chromatography of Ethylene/1-Alkene Copolymers Rajesh Chitta1, Tibor Macko1, Robert Brüll1, Prabhu Kavimani Nagar1, Christophe Boisson2, Olivier Boyron2 1Fraunhofer Institute, LBF, Darmstadt, DE; 2University of Lyon, Lyon, DE

POLY17_TU Electrospun Poly(Co-Styrene) Solid Phase Extraction Sorbents for Alkylphenols Yolanda Tancu, Nelson Torto Rhodes University, Grahamstown, ZA

POLY18_WE Molecularly Imprinted Polymer (MIP) Dedicated to the Extraction of Glyphosate and its Metabolite, AMPA, in Water Kinga Puzio1, Berengere Claude1, Catherine Berho2, Emeline Grellet2, Laurence Amalric2, Sami Bayoudh3, Philippe Morin1 1ICOA – UMR 7311, Université d’Orléans, Orléans, FR; 2BRGM – Direction des laboratoires, Orléans, FR; 3POLYINTELL, Val de Reuil, FR

POLY19_MO Application of the General Theory of Gradient Elution to Characterization of Functionalized Polymers Christopher Rasmussen, Brian McCauley, Yefim Brun DuPont, Wilmington, US

POLY20_TU Quantitative Evaluation of the Evaporative Light Scattering Detector Response for Linear Polyethylene Jan-Hendrik Arndt, Tibor Macko, Robert Brüll, Kavimani Nagar Prabhu Fraunhofer Institute, LBF, Darmstadt, DE

POLY21_WE Multivariate Curve Resolution Alternating Least Squares for Improved SEC-DAD Deconvolution of Complex Polymer Samples Tim Van Hoeylandt, Kai Chen, Filip Du Prez, Frédéric Lynen Ghent University, Ghent, BE

POLY22_MO Comprehensive Characterization of Copolymers and Polymer Mixtures Containing Polymers of Different Polarities Elena Uliyanchenko, Christian Wold, Jan Henk Kamps, Tanzeela Raja SABIC, Bergen op Zoom, NL

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Proteomics PROT01_MO Intact Protein LC-MS, How to Overcome the Challenges? Mauro De Pra1, Evert-Jan Sneekes1, Laurent Rieux1, Christian Ravnsborg2, Dafydd Milton3, Remco Swart1 1Thermo Fisher Scientific, Amsterdam, NL; 2Thermo Fisher Scientific, Odense, DK; 3Thermo Fisher Scientific, Runcorn, GB

PROT02_TU Simple Two-Dimensional Separation Platform for Peptide Analysis Filip Duša, Josef Šesták, Dana Moravcová, Josef Planeta, Vladislav Kahle Institute of Analytical Chemistry of the ASCR, v. v. i., Brno, CZ

PROT03_WE High Performance Thin Layer Chromatography Nanospray Mass Spectrometry Using the Advion Nanomate® System Hans Griesinger, Susanne Minarik, Bernhard Schubach, Katerina Matheis, Michael Schulz Merck KGaA, Darmstadt, DE

PROT04_MO Solid-Phase Based Technologies and Methods for Glycoproteome and Phosphoproteome Analysis Zou Hanfa, Fangjun Wang, Mingliang Ye, Jun Zhu, Zhen Sun, Hongqiang Qin, Junfeng Huang Dalian Institute of Chemical Physics, Dalian, CN

PROT05_TU Charged Surface Hybrid C18 for High Resolution Peptide Analyses and Lab Scale Peptide Purifications Matthew Lauber, Stephan Koza, Pamela Iraneta, Kenneth Fountain Waters Corporation, Milford, US

PROT06_WE Electrochemically Assisted Reduction of Disulfide Bonds in Peptides and Proteins with On-Line Mass Spectrometric Detection Agnieszka Kraj, Martin Eysberg, Hendrik-Jan Brouwer, Jean-Pierre Chervet Antec, Zoeterwoude, NL

PROT07_MO Quantitative Proteomic Profiling with HPLC-MS/MS: Comparison of Various Labelling Strategies Using Itraq and TMT Theresa Kristl1, Marc Rurik2, Angela Schmiedlechner1, Albert Duschl1, Oliver Kohlbacher2, Christian Huber1 1University of Salzburg, Salzburg, AT; 2University of Tübingen, Tübingen, DE

PROT08_TU Characterization of an Immobilized Trypsin PLOT Column Used in an On-Line LC-MS System Hanne Kolsrud Hustoft, Ole Kristian Brandtzæg, Steven Ray Wilson, Tyge Greibrokk, Elsa Lundanes Department of Chemistry, Oslo, NO

PROT09_WE A Proteomics Screening Method for the Identification of Antimicrobial Active Molecules in Colombian Snake Venom Andres M. Tibabuzo, Jackson Ocampo, Barbara H. Zimmermann, Chad Leidy, Roland J.W. Meesters Universidad de los Andes, Bogota D.C., CO

PROT10_MO Peptide Separation on a Normal Phase Column Under HILIC Separation Conditions Goran Mitulović, Andreas Fichtenbaum, Rainer Schmid Medizinische Universität Wien, KILM, Wien, AT PROT11_TU High-Resolution Peptide Separations Using Nano-Liquid Chromatography At Ultra- High Pressure Lucie Novakova1, Axel Vaast2, Catherine Stassen2, Ken Broeckhoven2, Mauro De Pa3, Remco Swart3, Sebastiaan Eeltink2 1Charles University in Prague, Hradec Králové, CZ; 2Vrije Universiteit Brussel, Brussel, BE; 3Thermo Fisher Scientific, Amsterdam, NL

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PROT12_WE A Natural Point Mutation Changes Both Target Selectivity and Mechanism of Action of Sea Anemone Toxins. Steve Peigneur1, László Béress2, Carolina Möller3, Frank Marí3, Wolf-Georg Forssmann4, Jan Tytgat1 1University of Leuven, Leuven, BE; 2Hannover Medical University, Hannover, DE; 3Florida Atlantic University, Boca Raton, US; 4Pharis Biotec GmbH, Hannover, DE

PROT13_MO Robust Phosphoproteome Enrichment Using Titanium (IV) Affinity Chromatography Houijang Zhou1, Harm Post1, Hanfa Zou2, Shabaz Mohammed1, Albert J.R. Heck1 1Utrecht University, Utrecht, NL; 2Chinese Academy of Sciences, Dalian, CN

PROT14_TU The Effects of HPLC-Analysis to Proteins and How to Affect These Effects for Precise and Reproducible Protein Analytic Tobias Rakow, Hermann Wätzig Technische Universität Braunschweig, Braunschweig, DE

PROT15_WE Specific Recognition of Tyrosine-Phosphorylated Peptides by Epitope-Imprinting Strategy Xueqin Ren, Yufeng Hu, Feng Shen, Qiushui Li, Lin Xu China Agricultural University, Beijing, CN

PROT16_MO Molecular Imprinted Polymers as New Tools in Small Cell Lung Cancer Diagnosis. Cecilia Rossetti1, Trine Halvorsen1, Abed Qader2, Börje Sellergren2, Léon Reubsaet1 1University of Oslo, Oslo, NO; 2Technical University of Dortmund, Dortmund, DE

PROT17_TU Optimized Superficially Porous Particles for Peptide and Protein Analysis Stephanie A. Schuster, Joseph J. Kirkland, Brian M. Wagner, Barry E. Boyes, Joseph J. DeStefano Advanced Materials Technology, Inc., Wilmington, DE, US

PROT18_WE High Performance Thin Layer Chromatography Mass Spectrometry Using the Camag TLC-MS Interface Axel-Walter Thomasberger, Hans Griesinger, Susanne Minarik, Katerina Matheis, Michael Schulz Merck KGaA, Darmstadt, DE

PROT19_MO Parameters Affecting the Gradient Reversed-Phase Separation of Peptides and Intact Proteins Axel Vaast, Eva Tyteca, Gert Desmet, Sebastiaan Eeltink Vrije Universiteit Brussel, Brussel, BE

PROT20_TU Proteomic Analysis of N- and O-Linked Glycosylation Based on Hydrophilic Interaction Chromatography Jun Zhu, Fangjun Wang, Kai Cheng, Mingliang Ye, Hanfa Zou Dalian Institute of Chemical Physics, CAS, Dalian, CN

PROT21_WE The Kinetic Study of Petide Digestion by Trypsin Tereza Šlechtová1, Květa Kalíková1, Martin Gilar2, Eva Tesařová1 1Charles University in Prague, Prague, CZ; 2Waters Corporation, Milford, US

PROT22_MO Contichrom: A Versatile Purification Platform with All-In-One Process Capabilities Guido Stroehlein, Thomas Mueller-Spaeth, Lars Aumann, Michael Bavand ChromaCon AG, Zuerich, CH

PROT23_TU Gold Nanoparticles Modified Hydrophilic Monoliths for High Selective Enrichment of Glycoproteins and Glycopeptides Yu Liang, Ci Wu, Lihua Zhang, Yukui Zhang Dalian Institute of Chemical Physics, CAS, China, Dalian, CN

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Quantitative MS QTMS01_MO Development and Validation of Reverse –Phase Liquid Chromatography –Time-Of- Flight / Mass Spectrometry Method for Simultaneous Analysis of Four Antiinflammatory Pharmaceuticals in Water. Fouad Al-Qaim1, Pauzi Mohamed1, Mohamed Othman1, Zuriati Zakaria2, Jalifa Latip1 1National University of Malaysia (UKM), Bangi, MY; 2Malaysian-Japan International Institute of Technology(MJIIT), Kuala Lumpur, MY

QTMS02_TU Optimization of a Transglycosylation Assay for S. Aureus PBP2 Bart Blanchaert, Erwin Adams, Ann Van Schepdael KU Leuven, Leuven, BE

QTMS03_WE Determination of Asymmetric and Symmetric Dimethylarginine in Serum from Patients with Chronic Kidney Disease: UPLC-MS/MS Versus ELISA Jente Boelaert1, Eva Schepers2, Griet Glorieux2, Raymond Vanholder2, Frédéric Lynen1 1Separation Science Group, Ghent University, Gent, BE; 2Renal Division, Ghent University, Gent, BE

QTMS04_MO High Performance Liquid Chromatography Mass Spectrometry for Simultaneous Determination of Polar Pollutants in Zebrafish Embryos Stephan Brox, Axel P. Ritter, Eberhard Küster, Thorsten Reemtsma Helmholtz- Centre for Environmental Research UFZ, Leipzig, DE

QTMS05_TU Determination of Residual Amounts of Aminoglycosides in Food Products of Animal Origin by Ultra High Performance Liquid Chromatography Coupled with High Resolution Mass Spectrometry Serguei Calugaru, Evgeny Khvatov, Pavel Metalnikov, Alexander Komarov VGNKI, Moscow, RU

QTMS06_WE Rapid Determination of Selected Artificial Sweeteners in Beverage Samples by Ultra Performance Liquid Chromatography with Photodiode Array and Tandem Mass Spectrometry Detection Antonia Mª Carro, Teresa Pena, Purificación Fernandez, Maria Regenjo, Paula Gonzalez, Rosa A. Lorenzo University of Santiago de Compostela, Santiago De Compostela, ES

QTMS07_MO Development, Validation and Application of a Method for the Analysis of Quaternary Ammonium Compounds (Qacs) in Dairy Products by LC-MS/MS Christoph Czerwenka, Eveline Dorn Austrian Agency for Health and Food Safety, Wien, AT

QTMS08_TU Analysis of Perfluorinated Surfactants in Electroplating Industries by HPLC-MS/MS Ina Ebersbach1, Oliver J. Schmitz1, Joachim M. Marzinkowski2 1University Duisburg-Essen, Applied Analytical Chemistry, Essen, DE; 2University of Wuppertal, Wuppertal, DE

QTMS09_WE Fast UHPLC-MS/MS Method for Simultaneous Quantification of Erlotinib, OSI-420, Sunitinib, SU-12662, and Pazopanib in Dried Blood Spots (DBS) Massimiliano Donzelli1, Daria Winogradova1, Benjamin Berger1, Björn-Thoralf Erxleben2, Udo Burger3, Magdalena Swiderska4, Markus Joerger5, Stephan Krähenbühl1, Manuel Haschke1 1University Hospital Basel, Basel, CH; 2Shimadzu Europe, Duisburg, DE; 3Shimadzu Schweiz GmbH, Reinach, CH; 4Camag, Muttenz, CH; 5Kantonsspital St. Gallen, St. Gallen, CH

QTMS10_MO A Survey of the Occurrence of Ochratoxin A in Madeira Wines Based on a Modified Quechers Extraction Procedure Combined with Liquid Chromatography–Triple Quadrupole Tandem Mass Spectrometry Paulo Fernandes1, Nélson Barros1, Jorge Pereira2, José S. Câmara2 1Laboratório Regional de Veterinária e Segurança Alimentar, Funchal, PT; 2CQM, UMa, Funchal, PT

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QTMS11_TU Rhpda Probe for Nitric Oxide- UPLC/MS Analysis Ewa Gapys, Malgorzata Urbaniak, Jan Adamus, Adam Sikora, Andrzej Marcinek Lodz University of Technology, Lodz, PL

QTMS12_WE Analysis of Carnitine Precursors in Biological Matrices by Ultra Performance Liquid Chromatography –Tandem Mass Spectrometry (UPLC/MS/MS) Solveiga Grinberga, Eduards Sevostjanovs, Osvalds Pugovics, Edgars Liepins Latvian Institute of Organic Synthesis, Riga, LV

QTMS13_MO Validated LC-MS/MS Method for the Determination of Tranquilizers and Β-Blocker Residues in Animal Kidney Dominique Hurtaud-Pessel, Pierrick Couedor, Eric Verdon Anses, Laboratoire De Fougères, Fougeres, FR

QTMS14_TU Determination of Antifungal Drugs Illegally Added in Cosmetics by Ultra High Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry Yin Huo, Chao Ma, Hui Gao, Qisheng Zhong, Lingling Zhong, Song Zhan, Shinichi Kawano, Taohong Huang, Yuki Hashi Shimadzu (China) Co.,Ltd., Beijing, CN

QTMS15_WE Validation of an LC-MS/MS Method in Line with EU Decision 2002/657/EC for Triphenyl Methane Dyes in Fish Muammer Kaplan, Elmas Öktem Olgun, Öznur Karaoğlu Tubitak MRC Food Institute, Kocaeli, TR

QTMS16_MO Development of a Rapid LC-MS/MS Method for the Determination of Thyreostatic Drugs in Urine and Gland Tissue Muammer Kaplan, Elmas Oktem Olgun, Oznur Karaoglu Barış Mah. Dr. Zeki Acar Cad. No: P.K. 2, Kocaeli, TR

QTMS17_TU Ultra Performance Liquid Chromatography Coupled to Mass Spectrometry Determination of Salmeterol and its Main Degradation Product in a Pharmaceutical Dry Powder Inhaler Reginald Klinkenberg Galephar M/F, Marche-en-Famenne, BE

QTMS18_WE Comparison of Direct Large Volume Injection and Online-Solid Phase Extraction LC- MS for the Determination of Hormones in Different Water Bodies Sebastian Kowal1, Friedrich Werres1, Peter Balsaa1, Torsten Schmidt2, Kristina Lang2 1IWW Water Centre, Mülheim an der Ruhr, DE; 2university duisburg essen, Essen, DE

QTMS19_MO Development of Chemical and Physical Methods to Examine a Multiple Traditional Chinese Herbal Prescription by LC-MS/MS, Electron Microscope and Congo Red Stained Chia-Ming Lu1, Mei-Ling Hou1, Lie-Chwen Lin2, Tung-Hu Tsai1 1National Yang-Ming University, Taipei, TW; 2National Research Institute of Chinese Medicine, Taipei, TW

QTMS20_TU Nano UPLC-ESI-MS/MS for the in Vivo Monitoring of Neuropeptides During Epileptic Seizures Katrien Maes1, Guillaume Béchade2, Jolien Van Schoors1, Yvan Vander Heyden1, Yvette Michotte1, Ilse Smolders1, Ann Van Eeckhaut1 1Vrije Universiteit Brussel, Brussels, BE; 2Waters, Zellik, BE

QTMS21_WE Ultraperformance Lc and Dried Blood Spot Sampling as Novel Tools in the Rapid Determination of Pharmacokinetic Parameters of Drug Substances. Krishna Mahabir, Hans-Gerd Janssen, Boudewijn Hollebrands, Pieter vd Pijl, Guus Duchateau Unilever R&D, Vlaadingen, NL

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QTMS22_MO Identification and Quantification of Lignans in Wine By-Products – in Marc and Grape Seeds Using Lc-Ms Iveta Maresova1, Jan Triska2, Martin Moos2, Nadezda Vrchotova2, Dana Jirotkova1 1University of South Bohemia, Faculty of Agriculture, Ceske Budejovice, CZ; 2Global Change Research Centre AS CR, Ceske Budejovice, CZ

QTMS23_TU LC-MS/MS Determination of Ibuprofen Enantiomers in Human Serum Using Automated SPE Extraction Natalija Nakov, Liljana Bogdanovska, Ljiljana Ugrinova, Zoran Kavrakovski, Aneta Dimitrovska, Rumenka Petkovska Faculty of Pharmacy, University "Ss Cyril and Methodius", Skopje, MK

QTMS24_WE Validation and Application of an UPLC-MS/MS Method for a PK Study of an Anti- Allergic Indolinone Derivative in Rat Plasma Mouhssin OUFIR1, Veronika Butterweck2, Chethan Sampath3, Daniela Eigenmann1, Matthias Hamburger1 1Division of Pharmaceutical Biology, University of Basel, Basel, CH; 2Institute of Pharma; Technology, University of Applied Sciences Northwestern Switzerland, Muttenz, CH; 3Department of Pharmaceutics, University of Florida, Gainesville FL, US

QTMS25_MO LC-MS and LC-MS/MS for Determination of Water-Soluble Vitamins in Foods Melissa Phillips, Catherine Rimmer, Lane Sander NIST, Gaithersburg, US

QTMS26_TU Rapid and Robust Analysis Method for Quantifying Antidepressants and Major Metabolites in Human Serum by UHPLC-MS/MS Christ Pijnenburg1, Vincent Goudriaan2, Jacob Diepenbroek1, Jan Giesbertsen1, Annemieke Vermeulen Windsant-v.d. Tweel1 1ZANOB BV, 's-Hertogenbosch, NL; 2Shimadzu Benelux BV, 's-Hertogenbosch, NL

QTMS27_WE Quantitation of Central Carbon Metabolites in Human Blood Spots by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Estitxu Rico1, Oskar González2, María Encarnación Blanco1, Rosa María Alonso1 1University of the Basque Country, Leioa, ES; 2Leiden University, Leiden, NL

QTMS28_MO Quantitative Determination of Fentanyl in Newborn Pig Plasma and Cerebrospinal Fluid by HPLC-MS/MS M. Encarnación Blanco1, Oskar González2, Estitxu Rico1, Rosa María Alonso1, Esther Encinas1, Elena Suárez1 1Universidad del País Vasco/EHU, Leioa, ES; 2Leiden University, Leiden, NL

QTMS29_TU Quantification of Dabigatran in Human Plasma by LC-MS/MS Yuko Rönquist-Nii1, Anton Pohanka1, Rickard E. Malmström2, Olof Beck1 1Karolinska University Hospital, Huddinge, Stockholm, SE; 2Karolinska Institutet & Karolinska University Hospital, Stockholm, SE

QTMS30_WE A Bioanalytical Study on Two Thiosemicarbazone Antineoplastic Agents, DPC and DP44MT, in Rats Using LC-MS Vit Sestak1, Jan Stariat1, Vlasta Suprunova1, Stanislav Micuda2, Des Richardson3, Petra Kovarikova1 1Faculty of Pharmacy, Charles University, Hradec Kralove, CZ; 2Faculty of Medicine in HK, Charles University, Hradec Kralove, CZ; 3Department of Pathology and Bosch Institute, University of Sydney, Sydney, AU

QTMS31_MO Micro Ultra High Pressure Liquid Chromatography - Mass Spectrometry for the Quantification of Pharmaceuticals Using Dried Blood Spot Sample Collection Format Kyoko Watanabe1, Neil Loftus2, Emmanuel Varesio1, Gérard Hopfgartner1 1University of Geneva, Geneva, CH; 2Shimadzu Corporation, Manchester, GB

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QTMS32_TU Determination of Proanthocyanidins in Litchi (Litchi Chinensis Sonn.) Flower by HPLC-DAD-ESI/MS Deng-Jye Yang1, Jau-Tien Lin1, Yan-Zin Chang1, Yi-Chen Chen2, Shih-Chuan Liu1, Chuan-Hsiang Hsu1 1Chung Shan Medical University, Taichung, TW; 2National Taiwan University, Taipei, TW

QTMS33_WE Specific Enrichment of 3-Nitrotyrosine–Containing Peptides for Biomarker Discovery Yun Yang, Sara Ongay, Ido Kema, Rainer Bischoff University of Groningen, Groningen, NL

QTMS34_MO Analysis of Gluconate in Radioactive Wastes Prior to their Disposal Laurence du Bois de Maquillé1, Laetitia Renaudin1, Florence Goutelard1, Alain Jardy2, Jérome Vial2, Didier Thiébaut2 1CEA Saclay, France, FR; 2ESPCI-LSABM, France, FR

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Retention Mechanisms RMEC01_MO Adamantyl-Group Containing Mixed-Mode Acrylamide-Based Continuous Beds for Capillary Electrochromatography Ayat Allah Al-Massaedh, Ute Pyell University of Marburg, Marburg, DE

RMEC02_TU Preparation and Characterization of Stationary Phases for HPLC Using Mixture of PDMS and PMODS Carla Grazieli Azevedo da Silva, Elias Silva Júnior, Carol Collins Institute of Chemistry/University of Campinas (UNICAMP), Campinas/SP, BR

RMEC03_WE Analysis of Glyphosate and AMPA in Environmental Water Samples by Ion Chromatography and on Line Mass Spectrometry Without Derivatization. Cees Bruggink, Frans Schoutsen Thermo Fisher Scientific, Breda, NL

RMEC04_MO Investigation of the Retention Mechanisms on Grafted Monolithic Columns Based on Adsorption Isotherms Ekaterina Davydova1, Rudy Vonk1, Muzi Li1, Patrik Forssén2, Torgny Fornstedt2, Peter Schoenmakers1 1University of Amsterdam, Amsterdam, NL; 2Karlstad University, Karlstad, SE

RMEC05_TU Influence of Pressure and Temperature on Molar Volume and Retention Properties of Peptides in Ultra-High Pressure Liquid Chromatography Szabolcs Fekete1, Krisztian Horvath2, Davy Guillarme1 1University of Geneva, Geneva, CH; 2University of Pannonia, Veszprem, HU

RMEC06_WE Hydrophilic Interaction Liquid Chromatography as an Alternative Method for the Determination of Iodinated X-Ray Contrast Media Nuria Fontanals, Daniela Salas, Francesc Borrull, Rosa Maria Marcé Universitat Rovira i Virgili, Tarragona, ES

RMEC07_MO Poly(3-Chloro-2-Hydroxypropyl Methacrylate) Based Reactive Monolith with Derivatization Flexibility as a New Separation Medium for Capillary Electrochromatography. Part I: Sulfonic Acid Functionalized Strong Cation Exchance Stationary Phase Aslihan Gokaltun, Cemil Aydogan, Bekir Celebi, Adil Denizli, Ali Tuncel hacettepe University, Ankara, TR

RMEC08_TU A New Approach to Increase Volume Loading for Analytical and Semi-Preparative RP Gradient Separations of Small Molecules Wolfgang Goetzinger, Shawn Ayube, Grace Bi, Larry Miller Amgen, Cambridge, US

RMEC09_WE Practical Observations on the Dynamic Performance of Bare Silica in HILIC Compared to RPLC James Heaton1, Xiaoli Wang2, William Barber2, Stephan Buckenmaier3, David McCalley1 1University of the West of England, Bristol, GB; 2Agilent Technologies, Delaware, US; 3Agilent Technologies, Waldbronn, DE

RMEC10_MO Polymethacrylate Monoliths Functionalized with Cysteine for Capillary Electrochromatography Applications Isabel Ten-Doménech, Ernesto Simó-Alfonso, Guillermo Ramis-Ramos, José Manuel Herrero- Martínez Dept. Analytical Chemistry, University of Valencia, Burjassot (Valencia), ES

RMEC11_TU Molecular Shape Recognition of Self-Assembled Molecular Gel-Grafted Silica Particles in RP-HPLC Noguchi Hiroki, Thiraporn Charoenraks, Makoto Takafuji, Hirotaka Ihara Kumamoto University, Kumamoto, JP 131

RMEC12_WE Combined Pore-Scale Modeling of Mass-Transport and Stochastic Simulation of Sorption in Chromatographic Packings Dzmitry Hlushkou, Anton Daneyko, Ulrich Tallarek Philipps-Universität-Marburg, Marburg, DE

RMEC13_MO Analysis of Marine Biotoxins, Paralytic Shellfish Poisoning (PSP) and Diarrheic Shellfish Poisoning Toxins (DSP) in Mussels, (Mytilus Galloprovincialis) Along the Namibian Central Coast. Dijerenge Kahe Johanna University of Namibia, Swakopmund, NA

RMEC14_TU Analysis of Anti-Oxidant Phenolic Compounds in Dentifrices by RP-HPLC in Different Column Temerature JungHyun Kim1, Jungin Kim2, Jeehyun Hwang3, Hyo Kwang Ahn3, Joo Young Lee3, Chang woog Kim4 1KAIST, Chemical and biomolecular engineering, Daejeon, KR; 2Kwandong University College of Medicine, Gangneung, KR; 3Department of Preventive Social Dentistry School of Dentistry, Kyung Hee University, Seoul, KR; 4Gabriel Dental Clinic, Seoul, KR

RMEC15_WE Simultaneous Analysis of Carbapenems in Human Bodily Liquids Using HFIP as Buffer Additive in LC/ESI/MS/MS Karin Kipper1, Kristi Hüüdma1, Kadri Tamme2, Joel Starkopf2, Koit Herodes1 1Institute of Chemistry, University of Tartu, Tartu, EE; 2Clinic of Anaesthesiology and Intensive Care, University of Tartu, Tartu, EE

RMEC16_MO Comparison of the Mass-Transfer Properties of Insulin on Stationary Phases of Different Geometry in HPLC Ibolya Kiss, Nándor Lambert, Ferenc Kilár, Attila Felinger University Pécs, Pécs, HU

RMEC17_TU Development of a Hydrophilic Interaction Liquid Chromatography Method with Tandem Mass Spectrometric Detection for the Analysis of Pteridines in Two Graphosoma Species (Insecta: Heteroptera) Petr Kozlik1, Jan Krajicek1, Kveta Kalikova2, Eva Tesarova2, Zuzana Bosakova1 1Charles University, Department of Analytical Chemistry, Prague, CZ; 2Charles University, Department of Physical and Macromolecular Chemistry, Prague, CZ

RMEC18_WE Comparison of Three HILIC Approaches for the Determination of Pharmaceutical Residues by Electrospray Ionization – Tandem Mass Spectrometry (HILIC ESI- MS/MS) Paweł Kubica, Anna Jakimska, Agata Kot-Wasik, Andrzej Wasik, Jacek Namieśnik Gdansk University of Technology, Gdansk, PL

RMEC19_MO Large Volume Injection of Aqueous Samples in Nano Liquid Chromatography Using Serially Coupled Columns. Juri Leonhardt1, Thorsten Teutenberg1, Terence Hetzel1, Torsten C. Schmidt2 1Institute of Energy and Environmental Technology e.V. (IUTA), Duisburg, DE; 2University Duisburg-Essen, Instrumental Analytical Chemistry, Essen, DE

RMEC20_TU Substitution of Water by Methanol in Aqueous Normal Phase Liquid Chromatography Jan Soukup, Pavel Jandera University of Pardubice, Pardubice, CZ

RMEC21_WE Hydrophilic Liquid Chromatography with Ternary Mobile Phases: A Molecular Dynamics Study Versus Experimental Observations Sergey Melnikov1, Alexandra Hoeltzel1, Andreas Seidel-Morgenstern2, Ulrich Tallarek1 1Philipps-Universität, Marburg, DE; 2Max-Planck-Institut für Dynamik komplexer technischer Systeme, Magdeburg, DE 132

RMEC22_MO Computer-Assisted Modeling of Separation and Investigation of Dominant Retention Mechanism for Naturally Occurring Triterpenoids and Phytosterols Katerina Naumoska1, Zoran Kitanovski1, Irena Vovk2 1National Institute of Chemistry, Ljubljana, SI; 2National Institute of Chemistry; EN-FIST Centre of Excellence, Ljubljana, SI

RMEC23_TU Investigation of Stability and Determination of Contents for Catecins in Camellia Sinensis Containing Dentifrices Yong Duk Park1, Jung Eun Park1, Su Min Yoo2, Ji-Hwan Kim3, Ha-Jeong Kwon4 1Department of Preventive Social Dentistry, Kyung Hee Univ., Seoul, KR; 2Department of Dental Hygiene, Dong-Woo College, Sokcho, KR; 3College of Health Science, Korea University, Seoul, KR; 4Department of Preventive Social Dentistry, Kyung Hee Univ.,, Seoul, KR

RMEC24_WE A Comparison of the HPLC Retention Mechanisms on Silica Gel and Silica C Eman Santali1, Darren Edwards1, David Watson1, Oliver Sutcliffe2 1University of Strathclyde, Glasgow, GB; 2Manchester Metropolitan University, Manchester, GB

RMEC25_MO Molecular Theory of Size Exclusion Chromatography for Wide Pore Size Distributions Annamária Sepsey, Ivett Bacskay, Attila Felinger University of Pécs, Pécs, HU

RMEC26_TU Adsorption of Water−Acetonitrile Mixtures to Model Silica Surfaces Sergey Melnikov1, Alexandra Hoeltzel1, Andreas Seidel-Morgenstern2, Ulrich Tallarek1 1Philipps-Universität, Marburg, DE; 2Max-Planck-Institut für Dynamik komplexer technischer Systeme, Magdeburg, DE

RMEC27_WE Comparison of Fundamental Equations for Gradient Elution Chromatography José Ramón Torres-Lapasió, Juan José Baeza-Baeza, María Celia García-Álvarez-Coque University of Valencia, Burjassot (Valencia), ES

RMEC28_MO Education in Liquid Chromatography in the Lab. Poster Presentation Wim Staal1,2 1Hogeschool van Arnhem en Nijmegen, Hoogerheide, NL; 2HAN University of Applied Sciences, Nijmegen, NL

RMEC29_TU Ion-Pair UHPLC Analysis of Monoamines: Peak-Splitting At High Flow Rates Jolien Van Schoors1, Hendrik-Jan Brouwer2, Katrien Maes1, Yvette Michotte1, Ann Van Eeckhaut1 1Vrije Universiteit Brussel, Jette, Brussels, BE; 2Antec BV, Zoeterwoude, NL

RMEC30_WE Adapting the Surface Chemistry of Organic Monolithic Stationary Phases Using a Central Composite Design for the Optimization of an 2-Step Photografting Approach R.J. Vonk1, M. Li1, S. Eeltink2, G. Vivó-Truyols1, P.J. Schoenmakers1 1Universiteit van Amsterdam, Amsterdam, NL; 2Vrije Universiteit Brussel, Brussel, BE

RMEC31_MO Effect of Buffer Salt Concentration in Hydrophilic Interaction Chromatography on a Zwitterionic Stationary Phase Caroline West, Emeline Auroux ICOA, CNRS UMR 7311, University of Orléans, Orléans, FR

RMEC32_TU Application of Amino Stationary Phases for the Analysis of Carbohydrates Mark Woodruff, Neil McGrath Fortis Technologies Ltd, Neston, GB

RMEC33_WE Prediction of Overloaded Profiles in Gradient Elution Chromatography Dennis Åsberg1, Marek Lesko2, Martin Enmark1, Jörgen Samuelsson1, Patrik Forssén1, Krzysztof Kaczmarski2, Torgny Fornstedt1 1Department of Engineering and Chemical Sciences, Karlstad, SE; 2Rzeszow University of Technology, Rzeszow, PL 133

Sample Preparation SAMP01_MO Development and Evaluation of Quechers-Based Extraction and HPLC Method for Quantifying of Synthetic Food Dyes in Confectionery Samples Martin Adam, Petr Česla, Aleš Eisner, Andrea Čížková, Karel Ventura University of Pardubice, Department of Analytical Chemistry, Pardubice, CZ

SAMP02_TU Determination of Enzimatic Degradation Products of N-Homocysteinylated Proteins in Plasma by High Performance Liquid Chromatography Kamila Borowczyk, Edward Bald, Rafał Głowacki University of Lodz, Faculty of Chemistry, Dep. Env. Chem., Lodz, PL

SAMP03_WE Surfactant-Modified Sorbents and their Applications for Extraction of Carbamate Insecticides Prior to Analysis by HPLC Rodjana Burakham, Prapha Arnnok Khon Kaen University, Khon Kaen, TH

SAMP04_MO Analysis of Various Matrix Containing Food Samples Utilizing Robust Monolithic Silica Column Technology and LC-MS Karin Cabrera, Stephan Altmaier Merck KGaA, Darmstadt, DE

SAMP05_TU Automated Determination of Urinary Folate Metabolites P- Acetamidobenzoylglutamate and P-Aminobenzoylglutamate in Urine by HPLC- MS/MS Through in Situ Propyl Chloroformate Mediated Derivatization – Liquid Liquid Extraction Jana Cimlova1, Gry Kvalheim2, Per Magne Ueland3, Petr Simek1 1Biology Centre, AS CR, Ceske Budejovice, CZ; 2Institute of Medicine, University of Bergen, Bergen, NO; 3Haukeland University Hospital, Bergen, NO

SAMP06_WE Stir Bar Sorptive Extraction-Liquid Chromatography Tandem Mass Spectrometry Method for the Determination of Tributyltin Species in Seawater Noemí Dorival-García1, Francisco Javier Camino-Sánchez1, Alberto Zafra-Gómez1, Bartolomé Oliver-Rodríguez1, I Ruíz-Naranjo2, I Ruíz-García2, José Luis Vílchez1 1University of Granada, Granada, ES; 2Certio Environment S.L.TM, Granada, ES

SAMP07_MO Method Optimisation in Bottom-Up Analysis of Proteins Anthony Edge, Luisa Pereira Thermo Fisher Scientific, Runcorn, GB

SAMP08_TU Miniaturized Solid Phase Extraction Coupled to Capillary Liquid Chromatography for Online and Efficient Determination of Hormones At Low Levels in Water Supplies Rodrigo Padovan, Maraíssa Franco, Lucas Carvalho, Álvaro Santos-Neto, Fernando Lanças, Eduardo Azevedo University of São Paulo/Institute of Chemistry of São Carlos, SAO CARLOS, BR

SAMP09_WE Comparison of the Efficiency of Different MEPS Sorbents for the Extraction of the Fungal Metabolites Deoxynivalenol and Zearalenone Priscila Figueira1, Paulo Fernandes2, Jorge Pereira1, José S. Câmara1 1CQM, UMa, Funchal, PT; 2Laboratório Regional de Veterinária e Segurança Alimentar, Funchal, PT

SAMP10_MO Boron Nitride, a Novel Material for the Enrichment and Desalting of Tryptic Digest and the Depletion of Proteins Martin Fischnaller, Rainer Koeck, Rania Bakry, Guenther K. Bonn Analytical Chemistry and Radiochemistry, Innsbruck, AT

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SAMP11_TU Magnetic Bead Based Novel Sample Preparation Method for Automated N- Glycosylation Analysis of Biotherapeutics by Capillary Electrophoresis Csaba Varadi1, Clarence Lew2, Hans Dewald2, James Thorn2, Andras Guttman1 1Horvath Laboratory of Bioseparation Sciences, Debrecen, HU; 2Beckman Coulter, Brea, US

SAMP12_WE Selective Determination of Illicit Drugs and Pharmaceuticals in Wastewaters by a Novel Strong Cationic Exchange Solid-Phase Extraction Coupled to Liquid Chromatography and Tandem Mass Núria Gilart1, P.A.G. Cormack2, Rosa Maria Marcé1, Núria Fontanals1, Francesc Borrull1 1Universitat Rovira i Virgili, Tarragona, ES;2University of Strathclyde, Glasgow, GB

SAMP13_MO A New Stationary Phase for Solid-Phase Extraction with Marshmallow-Like Silicone Monoliths Gen Hayase1, Kei Morisato2, Kazuyoshi Kanamori1, Kazuki Nakanishi1 1Kyoto University, Kyoto, JP; 2GL Sciences Inc., Saitama, JP

SAMP14_TU Towards Unbiased Dried Blood Spot Analysis Using Temperature-Enhanced Flow- Through Desorption Coupled Online to Solid Phase Extraction and Mass Spectrometry Christel Hempen, Lena Knegt, Bert Ooms Spark Holland, Emmen, NL

SAMP15_WE Enzyme-Assisted Extraction of Proteins from Olive Fruit and Leaves María Vergara-Barberán, Cecilia Bataller-Fayos, Vicente Sáez-Montaner, Ernesto Francisco Simó-Alfonso, José Manuel Herrero-Martínez Dept. Analytical Chemistry, University of Valencia, Burjassot (Valencia), ES

SAMP16_MO Novel New Pseudo-Solvent Cloud-Point Microextraction for the Rapid Determination of Alkylphenols in Waters by High-Performance Liquid Chromatography and Ultraviolet Detection Jen-Fon Jen, Hou-Kung Shih, Ting-Yun Shu National Chung Hsing University, Taichung, TW

SAMP17_TU Development of an Analytical Method for Determination of Pesticides Used in the Cultivation of Cucumbers, Using Ultra High Performance Liquid Chromatography Luana C. Macedo, Elisa C. Morais, Isabel C. S. F. Jardim UNICAMP, Campinas, São Paulo, BR

SAMP18_WE Miniaturized Salting-Out Liquid-Liquid Extraction in Coupled-Syringes Combined with HPLC-UV for Extraction and Determination of Sulfanilamide Marzieh Khosraviani, Hassan Sereshti University of Tehran, Tehran, IR

SAMP19_MO Application of Lewis Acid/Base Interactions of Zirconia Based Sorbents for Sample Extraction Michal Klivicky, Radim Kucera, Jiri Klimes Charles University in Prague, Phaculty of pharmacy, Hradec Kralove, CZ

SAMP20_TU Automated Liquid-Liquid Extraction (LLE) of Drugs from Plasma Syed Lateef, Sundaram Palaniswamy, Siji Joseph, Vinayak AK Agilent Technologies, Bangalore, IN

SAMP21_WE Target 2D UHPLC-MS/MS Quantification of Rifampicin and 25-Desacetylrifampicin in Rat Liver Microsomes Bianca Lopes, Josiane Cardoso, Bianca Silva, Quezia Cass, Regina Vincenzi UFSCar, Sao Carlos, BR

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SAMP22_MO Dispersive Liquid–Liquid Microextraction Applied to Extraction of Benzodiazepines in Urine Followed by Ultra Performance Liquid Chromatography with Photo Array Detector (UPLC-PAD) Rosa A. Lorenzo1, María Regenjo1, María Teresa Pena1, Antonia Mª Carro1, Ana Mª Fernandez2, Purificación Fernández1 1University of Santiago de Compostela, Santiago de Compostela, ES; 2Unidade Asistencial de Drogodepencias, Monforte de Lemos (LUGO), ES

SAMP23_TU Simultaneous PAH and PCB Extraction from Soil and Tissues with Accelerated Solvent Extraction Aaron Kettle, Linda Lopez, Frank Hoefler Thermo Fisher Scientific, Sunnyvale, CA, US

SAMP24_WE Solid Phase Extraction of Fluoroquinolone Antibiotics from Wastewaters – Assessment of Different Commercial Sorbents Alexandra S Maia1, Ana R Ribeiro2, Paula ML Castro3, Maria Elizabeth Tiritan4 1CICS-ISCSN-CESPU, CBQF-ESB-UCP, Gandra - Paredes, PT; 2CICS-ISCSN-CESPU, CBQF-ESB-UCP, CEQUIMED-UP, Gandra - Paredes, PT; 3CBQF-ESB-UCP, Porto, PT; 4CICS-ISCSN-CESPU, CEQUIMED-UP, Gandra - Paredes, PT

SAMP25_MO Validation and Application of a Method for Determination of Pesticide Residues in Samples of Green Pepper Using Quechers and HPLC-MS/MS Elisa Morais, Luana Macedo, Isabel Jardim Unicamp, Campinas, BR

SAMP26_TU Novel Double-Confined Polymeric Ionic Liquids as Sorbents for Solid-Phase Microextraction with Enhanced Stability in High-Ionic-Strength Solution Juanjuan Feng, Hongdeng Qiu, Shuai Wang, Xia Liu, Shengxiang Jiang Lanzhou Institute of Chemical Physics, Lanzhou, CN

SAMP27_WE Determination of Light Alcohols, Cellosolves, Alcoholic Components of Fragrances and Non-Ionic and Anionic Surfactants in Cleaners by Ion-Exchange Separation, Derivatization and Liquid Chromatography Laura Ripoll-Seguer, Miriam Beneito-Cambra, José Manuel Herrero-Martínez, Ernesto F. Simo- Alfonso, Guillermo Ramis-Ramos University of Valencia, Fac. of Chemistry, Burjassot, ES

SAMP28_MO Obtaining Partition Coefficients of Biological Compounds between Ionic Liquids and Water by Using the “Solvation Parameter Model” Mario Reta, Juan Manuel Padró, Leidy Bibiana Agudelo Mesa, Rocío Pellegrino Vidal Fac. de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, AR

SAMP29_TU Improved Recoveries of Planar Pesticides from Quechers Extracts of Fruits and Vegetables When Using an Engineered Carbon Material for Pigment Removal Dwight Stoll1, Conor Smith2, Doug Fryer2, Jonathan Thompson2 1Gustavus Adolphus College, St. Peter, US; 2United Science LLC, Center City, US

SAMP30_WE Detection of Trace Level Herbicides from Drinking Water, Surface Water and Ground Water by Automated Online SPE-LC Triple Quadruple MS/MS Analysis Sonja Schneider, Bettina Schuhn Agilent Technologies, Waldbronn, DE

SAMP31_MO Variables Influencing the Injection Solvent Effect in Reverse Phase Liquid Chromatography Bradley VanMiddlesworth, John Dorsey Dublin City University, Dublin, IE

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SAMP32_TU Surfactant-Assisted Emulsification Dispersive Liquid-Liquid Microextraction Combined with HPLC for Analysis of Cabamate Residues Jitlada Vichapong, Rodjana Burakham Faculty of Science,Khon Kaen university, Khon Kaen, TH

SAMP33_WE HPLC Method for Determination of Different Forms of Cysteine and Glutathione in Cucumber Leaves Monika Wyszczelska-Rokiel1, Grażyna Chwatko1, Elżbieta Kuźniak2, Ewa Gajewska2, Marzena Wielanek2, Rafał Głowacki1 1University of Łódź, Faculty of Chemistry, Dep. Env. Chem., Lodz, PL; 2University of Łódź, Faculty of Biology and Environmental Protection, Department of Plant Physiology and Biochemistry, Lodz, PL

SAMP34_MO Determination of 1,3-Thiazolidine Derivative of Cysteine and Pyridoxal 5’- Phosphate by HPLC with UV Detection Monika Wyszczelska-Rokiel1, Hieronim Jakubowski2, Rafał Głowacki1 1University of Łódź, Faculty of Chemistry, Dep. Env. Chem., Lodz, PL; 2Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, Newark, New Jersey and Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, PL

SAMP35_TU Automated Solid-Phase Extraction of Phthalates for Drinking Water Samples Alex Zhang1, Pranathi Pareti2, Linda Lopez2 1Thermo Fisher Scientific, Sunnyvale, US; 2Thermo FIsher Scientific, Sunnyvale, US

SAMP36_WE Solid-Phase Extraction Enrichment of Pahs in Water Using Tetraazacalix[2]Arene[2]Triazine Modified Silica Gel (N-Calix-Tmsg) Zhang Shusheng Zhengzhou University, Zhengzhou, CN

SAMP37_MO Evaluation of Direct Sample-Inlet Mass Spectrometry (ASAP) of Vanilla Flavourings Baudien te Nijenhuis, Wilma du Chatinier, Boudewijn Hollebrands, Hans-Gerd Janssen Unilever R&D Vlaardingen B.V., Vlaardingen, NL

SAMP38_TU Screening of Pesticides in Water Using SPE On Line Stefano Zaza, Stefano Lucini Shimadzu Italia, Milan (Italy), IT

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Selectivity SELE01_MO Comparative Evaluation of Reversed-Phase HPLC Columns for the Analysis of Darifenacin in the Presence of its Related Compounds Mufarreh Asmari1, Mohamed Hefnawy2, Mohamed Attwa3 1King Khalid University, Asir, SA; 2King Saud University, Ryiadh, SA; 3King Saud University, Riyadh, SA

SELE02_TU Investigating Retention Characteristics of a Mixed Mode Stationary Phase and the Enhancement of Monolith Selectivity for HPLC Danielle N. Bassanese1, Arianne Soliven2, Paul G. Stevenson1, Gary R. Dennis2, Neil W. Barnett1, Xavier Conlan1, R. Andrew Shalliker2 1Deakin University, Geelong, VIC, AU; 2University of Western Sydney, Parramatta, NSW, AU

SELE03_WE Efficient and Rapid Method Development with Hybrid Silica Based Material by Utilizing Particle Size, Temperature and Bonded Chemistry Friederike Becker1, Noriko Shoji2, Takashi Sato2, Takatomo Takai2, Naohiro Kuriyama2 1YMC Europe GmbH, Dinslaken, DE; 2YMC Co., Ltd., Komatsu, JP

SELE04_MO Development of Highly Selective LC/MS Assay for Vitamin D Metabolites David Bell1, Craig Aurand1, Hugh Cramer1, Anders Fridstrom2, Frank Michel1 1Sigma-Aldrich/Supelco, Bellefonte, PA, US; 2Sigma-Aldrich/Supelco, St. Gallen, CH

SELE05_TU Design of Surface Selectivities for Special Applications with Silica Monoliths Karin Cabrera, Peter Knoell, Gisela Jung, Anita Piper, Benjamin Peters, Klaus Kreher Merck KGaA, Darmstadt, DE

SELE06_WE Size Exclusion Chromatography of Biomolecules – Detector Combinations to Obtain Maximum Information with Minimum Effort Andrew Coffey Agilent Technologies UK Ltd, Church Stretton, GB

SELE07_MO Transferring Multiple Methods Into a Single Analytical Solution Using Biocides in Consumer Products as a Model Jane A. Cooper1, Marian Twohig2 1Waters Corporation, Manchester, GB; 2Waters Corporation, Milford, US

SELE08_TU HILIC, Mixed-Mode, and Reversed-Phase: A Study in Retention Mechanisms Chris Denicola, Ty Kahler, Sharon Lupo, Rick Lajke Restek Corporation, Bellefonte, PA, US

SELE09_WE HILIC and Mixed-Mode Retention of the Pentafluorophenyl Propyl (PFPP) Stationary Phase Ty Kahler, Sharon Lupo, Jason Thomas, Chris Denicola, Rick Lake Restek Corporation, Bellefonte, PA, US

SELE10_MO An HPLC-UV Method Supporting the Reactivity Assessment of IVA Acyl-Glucuronide Derivative Emmanuel Hardillier1, Béatrice Cautain1, Delphine Chevillon2, Colette Prevost2, Olivier Lacombe1 1Inventiva, Daix, FR; 2Laboratoires Fournier, Daix, FR

SELE11_TU Evaluation of Performance in Reversed-Phase, and Ion-Exchange Modes of the Flare C18/Amino Polymer/Nanodiamond Mixed-Mode Column Chuan-Hsi Hung1, Tom Kazarian2, Brett Paull2, Pavel Nesterenko2, Andrew Miles3, David Jensen3, Andrew Dadson3, Matthew Linford1 1Brigham Young University, Provo, US; 2University of Tasmania, Hobart, AU; 3Diamond Analytics, Orem, US

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SELE12_WE HILIC-Phase Selectivity Chart for Characterization of HILIC Stationary Phases Mohammed E. A. Ibrahim, Yang Liu, Charles A. Lucy University of Alberta, Edmonton, Alberta, CA

SELE13_MO Phenylpiracetam Diastereomers Separation on Normal Phase Achiral Phases Helena Kazoka, Agrita Sproge, Grigorij Veinberg, Maksim Vorona Latvian Institute of Organic Synthesis, Riga, LV

SELE14_TU HILIC and Mixed-Mode Retention of an Embedded-Polar Stationary Phase Ty Kahler, Sharon Lupo, Jason Thomas, Chris Denicola, Rick Lake Restek Corporation, Bellefonte, PA, US

SELE15_WE Green Chromatographic Fingerprinting: A Trade Off between Separation and Sustainability Cristiano Funari1, Renato Carneiro2, Alberto Cavalheiro3, Emily Hilder1 1Australian Centre for Research on Separation Sciences/UTAS, Hobart, AU; 2Department of Chemistry, Federal University of São Carlos São Carlos, Sao Carlos, BR; 3Chemistry Institute, São Paulo State University, Araraquara, BR

SELE16_MO Quality by Design in UHPLC Method Development Róbert Kormány1, Imre Molnár2, Hans-Juergen Rieger2 1Egis Pharmaceutical, Budapest, HU; 2Molnár-Institute, Berlin, DE

SELE17_TU Selective Preconcentration and Determination of a PPCP, Sulpiride in River Water by an Online SPE-LC-MS Using a Molecularly Imprinted Adsorbent Takuya Kubo1, Kenta Kuroda1, Ken Hosoya2, Koji Otsuka1 1Kyoto University, Kyoto, JP; 2Kyoto Prefectural University, Kyoto, JP

SELE18_WE Complementary Selectivity in Hydrophilic Interaction Liquid Chromatography (HILIC) on Columns with Densely Bonded Zwitterionic Phosphorylcholine and Sulfobetaine Functional Groups Petra Lewits1, Tobias Jonsson2, Wen Jiang2, Petrus Hemström2, Patrik Appelblad2, Lena Westin2 1Merck Millipore, Merck KGaA, Darmstadt, DE; 2Merck Millipore, Merck SeQuant AB, Umeå, SE

SELE19_MO Retention Indices of Some Monoaromatics Using a Multiparametric Method in Binary, Ternary and Quaternary Solvent RP-LC Systems Didaoui Linda, Hassina Larbi USTHB University, Alger, Algeria, Alger, DZ

SELE20_TU Analyze Pharmaceuticals by Mixed-Mode Chromatography Xiaodong Liu, Mark Tracy, Udayanath Aich, Christopher Pohl Thermo Fisher Scientific, Sunnyvale, US

SELE21_WE Use of High Liquid Performance Chromatography (HPLC) for Determination of Folic Acid in Tablets Lilian Grace Silva Solon, Eliane Goncalves Araujo, Janiele Mayara Ferreira de Almeida, Cicero Flavio Soares Aragao, Carlos Alberto Martinez-Huitle, Nedja Suely Fernandes UFRN, Natal - RN, BR

SELE22_MO Column Comparison and Method Development for the Analysis of Short-Chain Carboxylic Acids by Zwitterionic HILIC with UV Detection Gabriella Massolini1, Alice Pedrali1, Giorgio Marrubini1, Petrus Hemström2, Tobias Jonsson2, Patrik Appelblad2 1Department of Drug Sciences, University of Pavia, Pavia, IT; 2Merck SeQuant AB, Umeå, SE

SELE23_TU Development of Guidelines for HPLC Method Transfer to New 5µm Fused-Core Particles Frank Michel1, Richard A. Henry2, David S. Bell2, Carmen T. Santasania2, Hugh M. Cramer2 1Sigma-Aldrich, Taufkirchen, DE; 2Supelco/Sigma-Aldrich, Bellefonte, US

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SELE24_WE Rapid HPLC Method Development with C18 and Phenyl Columns Toshi Ono1, Ken Tseng1, Tsunehisa Hirose2, Kazuhiro Kimata2 1Nacalai USA, Inc., San Diego, US; 2Nacalai Tesque, Inc., Kyoto, JP

SELE25_MO Development of a Mip-Mspd-Meck Method for Analysis of Natural and Synthetic Steroids in Goat Milk Judith Gañán1, Sonia Morante2, Alejandrina Gallego3, Rosa María Garcinuño3, Pilar Fernández3, Isabel Sierra2 1Rey Juan Carlos University, Madrid, ES; 2School of Experimental and Technologies Sciences, Rey Juan Carlos University, Madrid, ES; 3Department of Analytical Sciences, Faculty of Sciences, National University of Distance, Madrid, ES

SELE26_TU Adsorption Thermodynamics of Phenylcarboxylic Acids Enantiomers on a Chiral Adsorbent with Grafted Eremomycin Antibiotic Elena Reshetova, Arseny Blinov Institute of Technical Chemistry, Ural Branch of RAS, Perm, RU

SELE27_WE Exploring HPLC / UHPLC Chromatographic Selectivity with Low and High Ph Eluents on a New Extended Ph Range Stable Silica-Based Stationary Phase Alan Mckeown1, Carl Zimmerman2 1Advanced Chromatography Technologies Ltd, Aberdeen, Scotland, Gb; 2Macmod Analytical Inc., Chadds Ford, Pa, US

SELE28_MO An Ultra Fast Liquid Chromatography Method Development Using Surface Response Techniques to Determine Betamethasone and Dexamethasone in Topical Dermatologic Creams Lilian Grace Silva Solon, Marcelo Vitor Paiva Amorim, Larissa Marina Pereira Silva, Rhadames Menezes Costa Rocha, Jose Renato Lima Costa, Cicero Flavio Soares Aragao UFRN, Natal, BR

SELE29_TU Generic Method Approach for Pharmaceutical Drug Discovery and Development Using Reversed-Phase Hydrophilic Interaction Liquid Chromatography with Universal Charged Aerosol Detection Frank Steiner, Michael Heidorn, Markus Martin Thermo Fisher Scientific, Germering, DE

SELE30_WE Exploring the Possibilities of One-Segment-Per-Component Strategies to Enhance the Selectivity of Multi-Segment Gradient LC Eva Tyteca, Kim Vanderlinden, Gert Desmet Vrije Universiteit Brussel, Elsene, BE

SELE31_MO Efficiency of Solvatic Sorbtion Model for Prediction of Multi-Step Gradient Elution in Reversed-Phase Liquid Chromatography Svetlana Vorslova1, Jelena Golushko2, Sergey Galushko2, Arturs Viksna1 1Department of Analytical Chemistry, University of Latvia, Riga, LV; 2Institute of Chromatography, Riga, LV

SELE32_TU Transfer of Methods in LC and UHPLC, What Calculations Do I Need? Mark Woodruff, Helen Lomax Fortis Technologies Ltd, Neston, GB

SELE33_WE Applications of Venusil AQ C18 in the Study of Pharmacokinetics and Bioavailability Zhu Xudong, Jack Liu Bonna-Agela, Tianjin, CN

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Sub- and Supercritical-Fluid Chromatography SSFC01_MO Implementation of Ultraperformance Supercritical Fluid Chromatography (UPSFC) for Chiral Separations Katrijn De Klerck, Debby Mangelings, Yvan Vander Heyden Vrije Universiteit Brussel, Brussels, BE

SSFC02_TU Supercritical-Fluid Chromatography for the Separation of Complex Bio-Oils Henrik Cornelisson-van-de-Ven1, B.W.J. Pirok1, Pieter Kooijman2, Andre van der Doel3, Noortje de Haan2, Dennis Drost1, Wim Genuit4, P.J. Schoenmakers1 1Van 't Hoff Institute for Molecular Sciences, Amsterdam, NL; 2VU University Amsterdam, Amsterdam, NL; 3Radboud University Nijmegen, Nijmegen, NL; 4Shell Research and Technology Centre, Amsterdam, NL

SSFC03_WE The Kinetic Plot Method in Supercritical Fluid Chromatography: Theoretical Validation and Evaluation of Constant Pressure Gradient Elution. Ruben De Pauw, Gert Desmet, Ken Broeckhoven Vrije Universiteit Brussel, Brussel, BE

SSFC04_MO Stationary Phase Optimized Selectivity Supercritical Fluid Chromatography (SOS- SFC) Sander Delahaye, Frédéric Lynen Ghent University, Gent, BE

SSFC05_TU Enantioselective Ultrahigh-Pressure Liquid Chromatography and Ultrahigh- Performance Supercritical Fluid Chromatography in the Chiral Science Separation Scenario: Ultra-Fast Separations on a New Sub-2-µm Whelk-O 1 Stationary Phase. Dorina Kotoni1, Alessia Ciogli1, Marco Pierini1, Jelena Kocergin2, Ted Szczerba3, Harald Ritchie4, Claudio Villani1, Francesco Gasparrini1 1Sapienza University of Rome, Rome, IT; 2Regis Technologies, Morton Grove (Illinois), US; 3Regis Technologies, Morton Grove (Illinoiso, US; 4Thermo Fisher Scientific, Runcorn, GB

SSFC06_WE Way to Extending SFC Application Scopes Chuping Luo, Ziqiang Wang Waters, New Castle, DE, US

SSFC07_MO Method Transfer of the United States Pharmacopeias’ Normal Phase Methods to Supercritical Fluid Chromatography Methods Syed Lateef, Sundaram Palaniswamy, Siji Joseph, Vinayak AK Agilent Technologies, Bangalore, IN

SSFC08_TU Optimal Column Geometry for High Kinetic Performance Supercritical Fluid Chromatography Using State-Of-The-Art Instruments. Alexandre Grand-Guillaume Perrenoud, Davy Guillarme, Jean-Luc Veuthey, Szabolcs Fekete School of Pharmacy, University of Geneva, Geneva, CH

SSFC09_WE UHPSFC-MS/MS : From Hyphenation Optimisation to High Sensitivity Analysis. Alexandre Grand-Guillaume Perrenoud, Jean-Luc Veuthey, Davy Guillarme School of Pharmacy, University of Geneva, Geneva, CH

SSFC10_MO Chromatographic Method Development Strategies for Convergence Chromatography Paula Hong, Michael Jones, Patricia McConville Waters Corporation, Milford, US

SSFC11_TU Application of the Isopycnic Kinetic Plot Method for Elucidating the Potential of Sub- 2 Micron and Core/Shell Particles in SFC Sander Delahaye1, Ken Broeckhoven2, Gert Desmet2, Frédéric Lynen1 1Ghent University, Gent, BE; 2Vrije Universiteit Brussel, Brussel, BE

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SSFC12_WE Stochastic Analysis of Mass-Transfer Properties in Supercritical Fluid Chromatography and in Liquid Chromatography Nándor Lambert, Felinger Attila University of Pécs, Pécs, HU

SSFC13_MO Ultraperformance Convergence Chromatography and State-Of-The-Art Stationary Phases Jacob Fairchild, Jason Hill, Pamela Iraneta, Darryl Brousmiche, Kevin Wyndham, Thomas Walter Waters Corporation, Milford, US

SSFC14_TU Ultra High Performance Supercritical Chromatography (UHPSFC) for Triglycerides Separation: Method Development. Eric Lesellier, Anna Latos ICOA, UMR 7311, Université Orléans, Orléans, FR

SSFC15_WE Development of a Strategy for Scaling SFC Methods: Applications for Preparative Chromatography Christopher Hudalla, Abhijit Tarafder, Kenneth Fountain Waters Corporation, Milford, MA, US

SSFC16_MO C18 Bonded Phases with Small Molecule Spacers: Supercritical Fluid Chromatography (SFC) Characterization and Carotenoid Test. Eric Lesellier1, Catherine Rimmer2, Lane Sander2 1ICOA, UMR 7311, Orléans, FR; 2NIST, Gaithersburg, US

SSFC17_TU Separation of Clenbuterol Enantiomers – A Comparison between HPLC and SFC- MS/MS Franz Liebetrau1, Maria Kristina Parr1, Alexander Schmidt2, Peter Witte1, Saskia Sterk3, Marco Blokland3 1Institute of Pharmacy, Freie Universität Berlin, Berlin, DE; 2Steiner & Co. Deutsche Arzneimittelgesellschaft mbH & Co. KG, Berlin, DE; 3RIKILT Institute for Food Safety, Wageningen, NL

SSFC18_WE Evaluation of Core Shell Columns for Improved Separation of Pharmaceutical Compounds by SFC Toshi Ono1, William Farrell2, Nicole Aurigemma2, Norikazu Nagae3 1Nacalai USA, Inc., San Diego, US; 2Pfizer, Inc., San Diego, US; 3ChromaNik Technologies, Inc., Osak, JP

SSFC19_MO Method Development for the Separation of Sulfated Estrogens by Ultraperformance Convergence Chromatography (UPC2) Christopher Hudalla1, Kenneth Fountain1, Manisha Patel2, Mark Hardink2, Frank Riley2 1Waters Corporation, Milford, MA, US; 2Pfizer Inc., Groton, CT, US

SSFC20_TU Application of Ultraperformance Convergence Chromatography - UPC2 - in Pharmaceutical Development Mijo Stanic, Alexander H. Schmidt Steiner Pharmaceuticals, Berlin, DE

SSFC21_WE Rapid Analysis of Endogenous Steroids Using UPC2 MS/MS for Clinical Research Christopher Hudalla1, Andrew Peck1, Kenneth Fountain1, Stuart Chadwick2, Fiona Liddicoat2 1Waters Corporation, Milford, MA, US; 2Waters Corporation, Manchester, GB

SSFC22_MO Purification Using HPLC-MS and SFC-MS in a medicinal chemistry environment Johan Söderlund, Pernilla Korsgren, Annika Langborg Weinmann AstraZeneca R&D, RIA iMed, Mölndal, SE

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SSFC23_TU Rapid Enantiomeric and Diastereomeric Resolutions of Chiral Pesticides Using Ultra Performance Convergence Chromatography with UV Detection Marian Twohig1, Michael O Leary1, Jane A. Cooper2, Sherry C. Perine3, David R. Stubbs3 1Waters Corporation, Milford, US; 2Waters Corporation, Manchester, GB; 3Syngenta Crop Protection LLC, Greensboro, US

SSFC24_WE Rapid SFC Method for Separation of Chlorthalidone Enantiomers Jiří Vozka, Květa Kalíková, Eva Tesařová Faculty of Science, Charles University in Prague, Prague, CZ

SSFC25_MO Analysis of Metoclopramide and Related Impurities Using UPC2/MS Michael Jones Waters Corporation, Milford, MA, US

SSFC26_TU The Use of an Ionic Liquid Stationary Phase for the Separation of Ionic Liquids with Supercritical Fluid Chromatography Guor-Tzo Wei, Yu-Siou Hung, Po-Hseuh Chen National Chung Cheng Universit, Ming-Hsiung, TW

SSFC27_WE Comparison of Pentafluorophenyl Stationary Phases in Supercritical Fluid Chromatography Caroline West, Syame Khater, Eric Lesellier ICOA, CNRS UMR 7311, University of Orléans, Orléans, FR

SSFC28_MO Chiral and Achiral Reaction Monitoring with Sub 2µ Particle Columns with CO2 Chromatography Sean McCarthy, Michael Jones, Andy Aubin, Margaret Maziarz Waters, Milford, US

SSFC29_TU Comparison of Chlorinated Polysaccharide Chiral Stationary Phases in Supercritical Fluid Chromatography Syame Khater, Caroline West ICOA, CNRS UMR 7311, University of Orléans, Orléans, FR

SSFC30_WE Efficiency Study on Overloaded Columns Using SFC and Using Chiral Compounds and Stationary Phases John Whelan, Ziqiang Wang Waters Corp, New Castle, US

SSFC31_MO Consequences of the Transfer from SFC to HPLC Conditions on the Overall Performance of a Chiral Zwitterionic Ion Exchange Type CSP Denise Wolrab, Michal Kohout, Wolfgang Lindner University of Vienna-Department of Analytical Chemistry, Vienna, AT

SSFC32_TU Chiral HPLC-to-Chiral SFC: An Easy Transfer? Syame Khater1, Anne-Marie Lozach2, Isabelle Adam2, Eric Francotte2, Caroline West1 1ICOA, CNRS UMR 7311, Université d'Orléans, Orléans, FR; 2Novartis Institutes for BioMedical Research, Basel, CH

SSFC33_WE CO2 Recycling Study from a Efficiency and CFD Persperctive John Whelan, John Baugher, Ziqiang Wang Waters Corp, New Castle, DE, US

SSFC34_MO On the Reproducibility between Different Modern Supercritcal Fluid Chromatographic Systems Martin Enmark, Jörgen Samuelsson, Torgny Fornstedt Department of Engineering and Chemical Sciences, Karlstad, SE

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SSFC35_TU Determination of Adsorption Isotherms in SFC - Revival of LC Methods in Highly Efficient SFC Systems Jörgen Samuelsson, Martin Enmark, Torgny Fornstedt Karlstad University, Karlstad, SE

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Stationary Phases STPH01_MO Characterization of a Mixed-Mode Reversed Phase/Cation-Exchange Stationary Phase Prepared by Thermal Immobilization of Poly(Dimethylsiloxane) Onto the Surface of Silica. Endler Borges1, Melvin Euerby2 1Universidade Estadual de Campinas, Campinas, BR; 2Hichrom Ltd.,, Berkshire, GB

STPH02_TU Mucin-Based Stationary Phases as Potential Tool for the Characterization of Drug – Mucus Interaction Andrea Gargano1, Michael Laemmerhofer2, Tomas Leek3 1University of Vienna, Vienna, AT; 2University of Tuebingen, Tuebingen, DE; 3R&D Astra Zeneca, Mondal, SE

STPH03_WE Evaluation of HPLC Stationary Phases for Environmental Analysis Ken Butchart, Sandra Hanson

Fortis Technologies Ltd, Neston, GB

STPH04_MO Zwitterionic Monosized-Porous Beads for Ion Chromatography Gunes Kibar1, Saba Samatya2, Ali Tuncel1 1Hacettepe Uni, Ankara, TR; 2SIIRT UNI, SIIRT, TR

STPH05_TU Kinetic Performance Comparison of Fully and Superficially Porous Particles with a Particle Size of 5 µm: Intrinsic Evaluation and Application to the Impurity Analysis of Griseofulvin Getu Kahsay1, Ken Broeckhoven2, Gert Desmet3, Erwin Adams1, Deirdre Cabooter1 1KU Leuven, Leuven, BE; 2Vrije Universiteit Brussels, Brussels, BE; 3Vrije Universiteit Brussel, Brussels, BE

STPH06_WE Separation and Determination of Organic Acids in Energy Drinks Using Polar Endcapped Reversed-Phase Column Ha Jeong Kwon, Yoon Jong Oh, Yu mi Jung, Jung Eun Park Department of Preventive Social Dentistry, Kyung Hee Univ., Seoul, KR

STPH07_MO Simple, Fast and Accurate Quantitation of Human Plasma Vitamins and their Metabolites by Rapid Protein Precipitation Combined with Columns Using HPLC-UV, HPLC-FLD Or LC-MS/MS Shuguang Li, Jason Anspach, Sky Countryman, Erica Pike Phenomenex, Torrance, CA, US

STPH08_TU Varying the Porosity of PS/DVB Beads and its Influence on RP-HPLC Performance Kristian Lungfiel, Andreas Seubert Philipps Universität Marburg, Marburg, DE

STPH09_WE A Novel Fluorous Solid Phase Extraction Method for the Determination of Environmentally Relevant Perfluoro-Alkyl Acids in Waters Nicola Marchetti1, Alberto Cavazzini1, Luisa Pasti1, Francesco Dondi1, Aldo Lagana`2 1University of Ferrara, Ferrara, IT; 2University of Rome "La Sapienza", ROME (ITALY), IT

STPH10_MO Improved Sample Preparation and HPLC/MS Determination of Pesticides in Fat- Rich Vegetables Frank Michel1, Emily Barrey2, Katherine K. Stenerson2, Olga Shimelis2, Michael Ye2 1Sigma-Aldrich, Taufkirchen, DE; 2Supelco/Sigma-Aldrich, Bellefonte, US

STPH11_TU Application of On-Column Polymerization Method of Acrylic Acid on Silica Monolith to the Preparation of Particle Type HILIC Stationary Phases Tomoyuki Okada1, Haruyuki Tanigawa1, Nobuo Tanaka2, Tohru Ikegami1 1Kyoto Institute of Technology, Kyoto, JP; 2GL Science, Saitama, JP

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STPH12_WE Poly(Ionic Liquid)-Grafted Silica as Multi-Mode Stationary Phases for High- Performance Liquid Chromatography Hongdeng Qiu1, Shengxiang Jiang1, Makoto Takafuji2, Hirotaka Ihara2 1Lanzhou Institute of Chemical Physics, Lanzhou, CN; 2Department of Applied Chemistry and Biochemistry, Kumamoto University, Kumamoto, JP

STPH13_MO Enantioselective Adsorption of Ibuprofen Enantiomers on a Chiral Adsorbent with Grafted Eremomycin Antibiotic Elena Reshetova Institute of Technical Chemistry of Ural Branch of the Russi, Perm, RU

STPH14_TU Development of a Novel Stationary Phase for HPLC: Polymeric Ionic Liquids Kevin Roeleveld, Frank David, Frédéric Lynen Ghent University, Ghent, BE

STPH15_WE How to Determine the Experimental Isoeluotropic Conditions by Change of the RPLC Stationary Phase in Carrying Out Only Two Manipulations Alain Tchapla1, Didier Charbonneau2, Guillaume Estievenart2, Pauline Albisson2, Sihem Groni1, Sylvie Heron1 1Univ.Paris Sud/Groupe de Chimie Analytique de Paris-Sud, Orsay, FR; 2Interchim R & D, Montluçon, FR

STPH16_MO Chiral Chromatography on Solid Supported Ice Stationary Phase Satsuki Takahashi, Tetsuo Okada Tokyo Institute of Technology, Tokyo, JP

STPH17_TU A Simple Method for Resolution of 22 Amino Acids in LC Ken Butchart, Sandra Hanson Fortis Technologies Ltd, Neston, GB

STPH18_WE The Development of a New Hydrolytically Stable Stationary Phase for U(H)PLC: Column Development Matthias Ide1, Frédéric Lynen2, Pat Sandra3, Pascal Van Der Voort2 1University Ghent/Mesosol, Ghent, BE; 2University Ghent, Ghent, BE; 3University Ghent/RIC, Ghent/Kortrijk, BE

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Vendor seminars

Monday, June 17, 2013 13:00 – 14:00

13:00 – 14:00 Waters Corporation Auditorium

Ultimate Efficiency Unleashed: A New UltraPerformance Column Technology for Maximizing Resolution and Throughput Speaker: Nicole M. Cotta, Waters Corporation Milford, MA , US

13:00 – 14:00 Sigma-Aldrich Emerald

Improving UHPLC Performance: Views from Industrial and Academic perspectives Trends in HPLC Column Technology and their Application: From Small Particles and Long Columns Speaker: Dr. Stefan Lamotte, Competence Center Analytics, BASF SE, DE Achieving the Highest Efficiency by Reduction of the Extra-Column Volume in a UHPLC Instrument: A Case Study of the New Titan C18 Columns Speaker: Dorina Kotoni, PhD,Dept. of Drug Chemistry and Technologies, Sapienza University of Rome, IT Impact of Particle Designs for Achieving Performance in Liquid Separations Speaker: David S. Bell, PhD, Manager Pharmaceutical and Bioanalytical Research, Supelco, US

13:00 – 14:00 Thermo Scientific G102-103

New Developments in Chromatography and Mass Spectrometry for Better and Faster Analytics of Biopharmaceuticals Speakers: Wim Decrop, Senior Research Scientist, Thermo Fisher Scientific Amsterdam, NL and Prof. Mag. Dr. Christian Huber, Head of Research Group Huber, Department of Molecular Biology, University of Salzburg, AT Moderator: Dr. Remco Swart, Manager Biomolecule Solutions HPLC Business Unit, Thermo Fisher Scientific Amsterdam, NL 13:00 – 13:30 Wissenschaftliche Geraetebau Dr. Ing. H. Knauer G104-105

How Many Process Types Does Your LC System Support? Speaker: Dr. Matthias Lübbert

13:30 – 14:00 Avantor Performance Materials G104-105

Advantages of Using LC/MS Solvent Packaged in Schott Bottles in LC/MS Applications Speaker: Ivana Ivanovic, Marketing Manager Laboratory Products, Avantor™ Performance Materials, Deventer, NL

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Tuesday, June 18, 2013 9:00 – 9:30 and 13:00 – 14:00

9:00 – 9:30 Molnár-Institute for applied chromatography G104-105

General UHPLC Method Development and Separation Modelling within the QbD- Framework Speakers: Imre Molnár, Hans-Jürgen Rieger, Institute for Applied Chromatography, Berlin, DE Robert Kormány, EGIS Pharmaceutical, Budapest, HU

13:00 – 14:00 Thermo Scientific Auditorium

Increased Throughput Pharma R&D and QA Workflows with Maximum Versatility - How Generic Can a UHPLC Method for Polar Analytes be? Speakers: Dr. Frank Steiner, Thermo Fisher Scientific, Germering, DE Stephen Luke, Thermo Fisher Scientific, Runcorn, UK 13:00 – 14:00 Shimadzu Europe Emerald

Excellence in Science - Solutions and Innovations Speakers: Dr. Gesa Schad, Dr. Björn-Thoralf Erxleben, Dr. Francesco Cacciola, Mikaël Levi

13:00 – 14:00 AB SCIEX G102-103

The use of Microflow UHPLC in Food and Environmental analysis Speakers: Dr. Thorsten Teutenberg, Institut für Energie- und Umwelttechnik e.V. Bereich Forschungsanalytik, Duisburg, DE Dr. Stephen Lock, AB SCIEX, Warrington, Cheshire, UK

13:00 – 14:00 Phenomenex G104-105

Importance of Instrumentation in Fast Liquid Chromatography Speakers: Szabolcs Fekete, Davy Guillarme School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, CH Improved Separations of Large and Small Bio Molecules Using Core-Shell Particles Jason A. Anspach, Jeff Layne, Michael McGinley, Lawrence Loo, and Tivadar Farkas

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Wednesday, June 19, 2013 9:00 – 9:30 and 13:00 – 14:00

9:00 – 9:30 Molnár-Institute for applied chromatography G104-105

Evaluation of Method Robustness Speakers: Imre Molnár, Hans-Jürgen Rieger, Institute for Applied Chromatography, Berlin, DE Robert Kormány, EGIS Pharmaceutical, Budapest, HU

13:00 – 14:00 Merck Millipore Auditorium

Latest Advances in ‘Fast HPLC' and 'Separations of Polar Compounds'

Monolithic Technologies superior to Core Shell Materials New complementary Selectivities in HILIC Speaker: To be announced

13:00 – 14:00 Waters Corporation Emerald

Novel Applications of Ultraperformance Convergence Chromatography (UPC2) Speaker: Kenneth J. Fountain, Waters Corporation Milford, MA, US

13:00 – 14:00 Chiral Technologies Europe G102-103

3A review of Columns Used in Chiral Chromatography and their Application in HPLC and SFC Which Column Type and Selector to Choose? Speaker: Pilar Franco - Manager Technical Operations

13:00 – 14:00 Agilent Technologies G104-105

Orthogonality, Efficiency, Selectivity and Detection – Tools for Profiling, Detection and Identification of Unknown Compounds Speakers: Dr. Michael Frank, Marketing Manager, Dr. Udo Huber, LC Solutions Manager, Agilent Technologies, Waldbronn, DE

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Thursday, June 20, 2013 8:30 – 9:30 and 12:50 – 13:30

8:30 – 9:30 Agilent Technologies G104-105

The Agilent 1290 Infinity 2D-LC Solution – Get Your Most-Complex Samples Separated Speaker: Dr. Jens Trafkowski, Product Manager, Agilent Technologies, Waldbronn, DE Application of Heart-Cut and Comprehensive 2D LC Separation for Advanced Chemical Analysis Matthias Pursch, Technology Associate, Analytical Technology Center, Dow Deutschland, Rheinmuenster, DE Optimizing Two-Dimensional Liquid Chromatography Systems: Can We Have it All? Gabriel Vivo-Truyols, Peter Schoenmakers, Sjoerd van der Wal, University of Amsterdam, NL

12:50 – 13:30 Restek Corporation G102-103

A Simple and Directed Approach to Faster Separations Development Utilizing the Selectivity of Modern LC Phases Speakers: Ty Kahler, Chris Denicola, Ed Franklin Restek Corporation, Bellefonte, PA, US

12:50 – 13:30 Agilent Technologies G104-105

Increase Your Sensitivity - The Agilent 1200 Infinity Series Online SPE Solution Speaker: Dr. Anneke Mühlebach, Product Manager, Agilent Technologies, Waldbronn, DE Quantitation of Trace Level Antibiotics in Minnesota River Water and Waste Water Effluent Using an Automated, Integrated Online-SPE LC-MS/MS System Speakers: Prof. Dwight Stoll*, Dr. David C. Harmes and Prof. Kristine Wammer *Gustavus-Adolphus College, St. Peter, Minnesota, US

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Symposium proceedings

It has been a tradition to publish the proceedings of the HPLC symposia in the Journal of Chromatography A. The organizers of HPLC 2013 therefore strongly encourage participants presenting lectures or posters at HPLC 2013 to submit a full-length manuscript for potential inclusion in a regular issue of the journal followed by a virtual special issue devoted to HPLC 2013.

This is a totally new concept which essentially rules out possible delays in publication for contributors to a special issue and includes options for videos and additional (colour) photo’s which would not be included in a printed issue. Thus making a Conference special issue more complete and accessible than in the past.

 Papers are published individually in regular issues as soon as they are accepted.  Footnotes indicate at which Conference they were presented, and are the source for selection and linking to the virtual special issue.  Virtual special issue is prepared and placed on Journal homepage – with links to papers on Science Direct.  Preface and possible photo’s – with the added possibility of videos e.g. from poster sessions, available via virtual special issue. Points to note:  The deadline to submit your manuscript to the HPLC 2013 virtual conference issue of Journal of Chromatography A is 1st September 2013.  When preparing your manuscript please carefully follow the journal’s Guide to Authors, which you can find on the online submission site: http://ees.elsevier.com/chroma.  In your cover letter please mention that your manuscript is intended for the HPLC 2013 virtual issue.  All manuscripts will be subject to the usual selection process including the strict peer review procedure, therefore acceptance for presentation at the meeting is not a guarantee for publication in the journal.  A separate dedicated video website will also contain videos comprised of a PowerPoint presentation or graphics presented along with an audio commentary given by you, the presenter, or one of your colleagues. This is intended as part of the “ expanded” virtual issue, and will not be included in the regular issue of the journal. If you wish to submit your poster presentation via this method, please contact the undersigned for a template and further instructions. In order to deal with the manuscripts as fast and efficiently as possible, you are invited to submit your article via the journal’s online submission and reviewing system which is accessible at: http://ees.elsevier.com/chroma. Below please see instructions for submitting your manuscript:

To submit:  Go to: http://ees.elsevier.com/chroma  Click on the “Submit Paper” option from the top menu  Enter your user name and password (first time users will have to register)  After entering the title of your manuscript, please select HPLC 2013 as the ‘Article Type’  Follow the remaining step-by-step instructions to submit your paper If you experience any technical problems or have any technical questions during the submission process, please contact Elsevier Author’s Support: [email protected]. If you have any questions on the submission and reviewing process, please contact the Editorial Office: [email protected].

We sincerely hope you will wish to contribute to this exciting project, and would wish you every success with the submission and eventual publication of your paper(s).

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Information for Speakers and Poster Presenters

Information for speakers Please note that all oral presentations, including the technical (vendor) seminars, will be handled by the RAI Speakers Centre. This means that you will have to hand in your (power point) presentation at the Speakers Centre, preferably on the day before the time of your presentation. It will not be possible to run your presentation from your own laptop. Information for poster presenters

Poster board and mounting All posters are meant to be on display thoughout the conference. Poster mounting time for all posters: Monday 8:00 – 11:00. Poster removal time for all posters: Thursday 13:30 – 15:00. Poster boards are 98 cm (3’2”) wide and 2 m (6’6”) high. Poster that exceed the size of the boards will not be permitted. You will be referred to the poster-printing service (see below).

Each poster board will be labelled with the poster number. Make sure this number remains visible after mounting your poster.

Posters have to be removed on Thursday, 20 June before 15:00. All remaining posters will be discarded after 16:00.

Poster sessions Poster authors will be required to be present on the designated poster day from 16:00 – 17:00. Poster sessions are scheduled during the breaks from 11:00 – 11:30, 12:30 – 14:00 and from 16:00 – 17:00 on Monday, Tuesday and Wednesday and from 11:00 – 11:30 and 12:30 – 13:30 on Thursday.

Poster printing services In case you did not carry your poster with you (or lost it) on your trip to Amsterdam, the RAI Bussiness Centre offers the possibility to have your poster printed on-site • Size: A0 (84x119 cm, portrait) • Full color • Original: as pdf, prepared on A0 size • Price: 35 Euro

You can hand your PDF-file on USB for printing, at the RAI Business Centre (entrance Elicium floor -1). Opening hours 9:00 – 17:00 on Sunday. Payment: in cash or by credit card, when collecting your poster.

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Exhibition

List of Exhibitors Booth number AB SCIEX A8 Advanced Chromatography Technologies Ltd. (ACE) E15 Advanced Chemistry Development Inc. (ACD/Labs) A19 Advion U16 Agilent Technologies A9 Akzo Nobel PPC U7 Analytical Sales and Services, Inc. E2 Antec BV E25 Avantor Performance Materials E3 Biosolve D3 Bonna Agela Technologies Inc. E5 C&EN U1 Cecil Instruments Ltd. E7 Chiral Technologies Europe A7 ChromSword A2 CI Informatics Ltd. E4 DIKMA Technologies E10 Elsevier E24 F-DGS U4 Future Conferences U0ab, D0 GE Healthcare A11 GL Science U18 Hamilton Bonaduz AG E6 JSB Group U9 Knauer - Wissenschaftliche Geraetebau Dr. Ing. H. Knauer GmbH A15 LCGC E17 Macherey Nagel U19 Merck Millipore A12 Molnár Institute (for applied chromatography) E18 MS Vision U2 Nacalai Tesque A20 Peak Scientific Instruments U10 Phenomenex U20 Polyintell A6 Publishers/Media U00 Restek E13 RPD TOOL AG A14 SCAT Europe GmbH E22 Scharlab SL E11 SelectScience E12 LSI Schmidlin U11 Screening Devices BV U3 Sedere SAS E16 Shimadzu Europe GmbH A1 Showa Denko Europe – Shodex E9

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List of Exhibitors Booth number Sigma Aldrich International GmbH A10 Spark Holland BV A13 The Analytical Scientist U17 Thermo Scientific Inc. E1 Tosoh Bioscience GmbH U8 VICI AG International A4 VWR International GmbH A3 Waters Corporation D4 Wyatt Technology Europe GmbH E8 YMC Europe GmbH D1

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Exhibitors

Advanced Chromatography Technologies Ltd. (ACE) ACE® ultra-inert HPLC/UHPLC columns from Advanced Chromatography Technologies Limited are designed to meet the most challenging of chromatographic applications. A wide range of particles /pore sizes, bonding chemistries and column dimensions are available providing excellent reproducibility and robustness with acidic, basic and neutral molecules. NEW additions include the ACE® Excel™ Ultra Robust UHPLC column product family and the ACE® SuperC18™ extended pH range phase. ACE® Excel™ is available throughout the world in 2 µm particles and a wide range of phases including the ACE® C18-AR, ACE® C18-PFP and ACE® SuperC18™.

Advanced Chemistry Development Inc. (ACD/Labs)

Advion Advion is a leader in ultra-high performance, small footprint mass spectroscopy & synthesis solutions for life science, which deliver speed, flexibility, ease-of use, reliability and exceptional data quality. The expression CMS is a high performance mass spec, yet half the size and half the price of conventional single quadrupole systems. Its compact size allows it to be placed in a fume hood or on the bench for direct access and immediate results for chemists engaged in drug discovery requiring mass confirmation, reaction monitoring, QC and purity analysis. The expression CMS puts the power of performance where chemists need it.

Akzo Nobel PPC AkzoNobel offers Kromasil, high performance chromatographic media based on state-of-the- art spherical silica for analytical and industrial HPLC applications. Products are available in slurrypacked columns for lab analysis and small scale purification plus bulk material for pharmaceuticals' manufacturing. The new range of UHPLC columns completes Kromasil's line; scientists can now use the same quality products with unmatched ratio of resolution per analysis time as their counterparts in production. Kromasil is the premium choice for API purification for its outstanding mechanical and chemical stability and is available for a wide range of NP, RP, SCF and chiral applications.

Analytical Sales and Services, Inc.

Antec BV Antec is the world’s leading supplier of Electrochemical Detectors for (U)HPLC and Potentiostats for use in Electrochemistry (EC) as a reaction technique upfront MS. The Decade II™ electrochemical detectors (ECD) are known for their superior sensitivity and versatility. They fit with any (U)HPLC to perform LC/ECD. The ROXY™ EC systems is the ultimate Electrochemistry system that complements any MS to conduct on-line EC/(LC)/MS. All these instruments will be on display at the exhibition together with new accessories such as the SenCell™, ChipCell™, or dedicated new electrodes for specific chemical reactions in EC/MS.

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Avantor Performance Materials Avantor™ Performance Materials manufactures and markets high-performance chemistries and materials around the world under several respected brand names, including the J.T.Baker® and Macron Fine Chemicals™ brands. Avantor is an established global supplier of high-purity laboratory chemicals, including solvents, acids, solutions, salts, minerals and sugars. Avantor products are at work in research and production laboratories, pharmaceutical and environmental testing, industrial manufacturing and many other areas around the world, delivering the high quality and purity needed for demanding applications.

Biosolve For over three decades, we produce and distribute selected high purity solvents, reagents and formulations for the research, pharmaceutical and biotechnology industries, and are now a major supplier in this field. Bio-Lab, Biosolve and more recently Biosolve Chimie, our new plant established in Lorraine France, meet the latest quality and environmental ISO standards, manufacturing under stringent quality control processes and serving you with the highest quality products on the market. As our customer, you deserve excellent service, starting from the right packaging for your application through to swift and reliable delivery. We welcome you to contact us whenever you need our sound advice for quality, safety or product design, or to discuss the grade that best accommodates your special needs

Bonna Agela Technologies Inc. Bonna-Agela Technologies Inc. is a separation technology company that serves chemists and biochemists in the field of drug discovery, food analysis, drug testing, environmental analysis and chemical research. We offer a full line of products designed to meet your separation and purification needs from bulk separation media to chromatography columns, purification system, SPE cartridges and automated SPE workstation.

C&EN C&EN, the weekly newsmagazine of the chemical & life sciences worlds, reaches the largest group of scientists in the world-over 128,000 worldwide. More than 75,000 readers work in life sciences and 85% have purchasing and specifying authority with 80% reading only C&EN in their professional role. C&EN brings breakthrough R&D, technology and industry news to the life sciences, pharmaceutical and instrumentation industries. C&EN is the best way to reach your target audience with 20 instrumentation-specific editorial issues, an award winning mobile app, 13 ACS online journals, along with C&EN Online (cen-online.org).

Cecil Instruments Ltd. Cecil Instruments are leading designers and manufacturers of HPLC systems, Ion Chromatography systems and UV /Visible Spectrophotometers. Cecil Instruments' Adept and Q-Adept ranges of modular HPLC and IonQuest Ion Chromatography instrumentation may be configured to individual requirements. There is a wide range of low noise and low drift detectors, including the ultra-fast scanning UV/Visible WaveQuest, electrochemical and conductivity detectors. Other LC modules include the astoundingly reliable AutoQuest autosamplers, various pumps and column heater/chillers. These may all be used with third party LC systems. PC control is by Windows based PowerStream and/or DataStream software.

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Chiral Technologies Europe Chiral Technologies is the global leader in enantioselective chromatography, offering the largest portfolio of chiral stationary phases, analytical and preparative chiral columns, and custom separation services. Chiral Technologies serves the North and Latin American markets through Chiral Technologies Inc. (CTI) and the European market through Chiral Technologies Europe SAS (GTE), wholly-owned subsidiaries of Daicel Chemicals Industries Ltd.

ChromSword The ChromSword group is a leading provider of HPLC method development solutions for customers in the Life Science Research, Pharmaceutical and Chemical Industry. The ChromSword group provides analytical scientists worldwide with a range of software packages for computer assisted and fully automatic HPLC method development and support services. ChromSwordAuto is powerful, method development software capable of developing new methods or improving existing method fully automatically with HPLC instruments from different vendors including Agilent, Hitachi, Knauer, Thermo and Waters.

CI Informatics Ltd.

CI Informatics are European distributors for S-Matrix Corporation. We supply and support their Fusion AE platform, comprising of tools to support pharmaceutical development. These include: • LC Method Development • LC Method Validation • Fusion Product Development • Fusion Inhaler Testing (FIT)

We are also UK distributers for iCD supplying the Validat product to the UK market as well as iCD's Laboratory Information Management system, LABS/Q. In addition to these activities CI Informatics sell and support Galaxie CDS software and offer a wide range of related consultancy services. We are also Bruker re-sellers for the closely related Compass CDS platform.

DIKMA Technologies We are a company dedicated to developing innovative chromatography products including HPLC/UHPLC and GC columns, sample preparation products, bulk chromatographic media, reference chemicals, and chromatographic accessories and equipment. Our products are designed to meet the most challenging of chromatographic applications. A wide range of particle size, pore size, bonding chemistries and column dimensions are available. Excellent column performance and reproducible chromatography with acidic, basic and neutral molecules is ensured by the most stringent of validation protocols. Strong relationships with customers are a key component of our culture.

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F-DGS F-DGSi proposes innovating solutions in gas equipment. Don’t buy your gas, make it! The gas generators offer comfort, reliability, economy and safety. Hydrogen generators, without maintenance, pressure max. 16 bars, purity 99.99999%, ideal tool for carrier gas / FID applications. Nitrogen generator, with/without integrated compressor, of technology PSA and membrane enable us to match applications such as GC, ICP, COT, DEDL, SAMPLE EVAPORATION, LCMS. Zero air generators with/without integrated compressor produce a dry, clean air, without hydrocarbons for GC-FID. Ultra zero air generators, unique system, combine an air dryer and zero air generator to obtain purities of air even higher. Air dryers with/without CO2 are designed to match applications such as FT-IR, NMR, AA, TOC, rheometer etc. All of our laboratory gas generators offer superb technical performance and end user benefits in the lab, and, of course, our world- class service and support come as Standard. We feel confident that we offer the best gas generators on the market

GE Healthcare GE Healthcare Life Sciences provides tools for drug discovery, biopharmaceutical manufacturing and cellular technologies, so research scientists can be more productive, effective and creative. Our vision is to be the start-to-finish bioprocessing solution provider, the partner of choice in cell and protein research and the leader in life sciences services. Building on our broad expertise across life sciences, we are committed to helping researchers understand life at a molecular level and develop solutions that can prevent, diagnose, treat, and cure disease. GE Healthcare Life Sciences delivers breakthroughs in drug discovery, biopharmaceutical manufacturing and the latest in cellular technologies, so scientists and specialists discover new ways to predict, diagnose and treat disease.

GL Science GL Science is one of the leading suppliers of products for HPLC, LC/MS, Ultra HPLC, samples preparation, and proteomics consumables throughout the world. Visit GL Sciences to see and learn the latest developments in column and separation technology for your critical separation.

Hamilton Bonaduz AG Hamilton is a manufacturer of products designed for the measurement and handling of liquids. Microliter® syringes are outstanding in all areas of precision liquid handling. In the field of analysis Hamilton is renowned for its innovative laboratory and process electrodes such as pH, Redox, oxygen and conductivity sensors plus a superior selection of armatures, pH buffer and conductivity standards. Hamilton supplies HPLC columns and polymer- and silica-based bulk material. Completing the range are semi-automatic diluter/dispensers and SoftGrip TM pipettes. Hamilton also manufactures innovative robotic pipetting systems and OEM products for laboratories in the fields of biotechnology, analytical and pharmaceutical chemistry.

JSB Group

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LSI Schmidlin LNI Schmidlin SA, based in Geneva, Switzerland, create products for Air Pollution Monitoring, Emission & Process, Automotive, Laboratory and Medical Applications: Ozone Generators for low and high concentrations,

High precision components for Gas flow and pressure regulation, No maintenance Laboratory Hydrogen Generators, Hydrogen and Zero air 19" rack mounted systems for industrial application, Nitrogen Generators for GC and LCMS application, Zero Air and Ultra Zero Air Generators. To insure the highest quality, we has got ISO 9001-2008 and ISO 13458-2003 certifications, our Gas Flow Standard laboratory is ISO 17025 certified and our medical gas mixers are CE certified.

Macherey Nagel Since 1911 MACHEREY-NAGEL represents high quality, innovation and reliability in biomolecular and chemical analysis. Friendly expert advice as well as outstanding product quality have been the cornerstones of our corporate success for over 100 years. Our comprehensive line of laboratory supplies includes products for filtration, testing, water analysis, chromatography and bioanalysis. MACHEREY-NAGEL’s expertise in R & D and manufacturing of separation media is based on many years of experience in silica technology, thereby playing a pioneering role in separation science. Our research and manufacturing capabilities are the core competency enabling us to make your daily laboratory work easier.

MS Vision

Nacalai Tesque COSMOSIL chromatography product line includes analytical and preparative HPLC or UHPLC columns for low-molecular compounds, hydrophilic compounds, Saccharides, Proteins, Fullerenes.

Peak Scientific Instruments Peak Scientific Instruments Ltd are a manufacturer of laboratory Gas Generators including nitrogen, hydrogen and zero air suitable to operate most laboratory analytical applications such as LCMS (liquid chromatography mass spectroscopy) and GC (Gas chromatography). With varying flow rates, purities & pressures of gas generators, available with or without internal air compressors, Peak are confident to offer the complete solution to your total Gas requirements. Our Gas Generators provide a safe & economical alternative to combustible bottled Gas cylinders offering a constant purity resulting in accurate analysis.

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Polyintell Leader in development of molecularly imprinted polymers, POLYINTELL develops and manufactures from the commonly polymeric SPE products for a crude sample cleanup to the most innovative SPE for very specific extractions. POLYINTELL provides innovative extraction ready-to-use kits from complex media (food, biologic fluids,…) for various compounds such as Mycotoxins, Endocrine disruptors like Bisphenol A, Estogens, Antibiotics, Phenolics compounds, … under different formats. Visit and discover our latest innovations based on Molecularly Imprinted Polymers!

RPD TOOL AG

Scharlab SL Scharlab S.L. aim to be a global supplier of scientific solutions for chemical and microbiological laboratories and to improve and facilitate the work carried out by our customers. Scharlab is an internationally recognised company, manufacturing its own "Scharlau" brand of more than 2.000 laboratory chemicals and 400 culture media of microbiology. Headquarter in Barcelona (Spain) with subsidiaries in Poland, Hungary, Indonesia, Philippines and China, and network of distributors in more than 90 countries worldwide. Our investment in technology means we can offer high quality at competitive prices. Innovation and quality are a must in all of our activities.

Screening Devices BV

Sedere SAS SEDERE develops, manufactures, distributes and supports SEDEX detectors, the most complete and versatile product line dedicated to Low-Temperature Evaporative Light-Scattering Detection (LT-ELSD). As one of the originators of this detection mode, SEDERE remains exclusively focused on this technology as a core competency. As the industry leader, SEDERE leverages decades of experience and customer knowledge to continually raise the bar for High Sensitivity, High Flexibility and High Fidelity detector performance for chromatography laboratories. The unparalleled selection of three LT-ELSD models can match both very high performance requirements and budget for all chromatography applications from basic research to quality control.

Showa Denko Europe – Shodex Shodex™ is the brand name of the HPLC columns manufactured by Showa Denko KK in Japan. Our European headquarters with Sales and Customer Support are located in Munich, Germany. We have the largest market share in Japan with our advanced polymer-based columns. • Experts in polymer, sugar and protein analysis • Polymer-based and silica-based LC columns • Product portfolio: IC, IEX, RP, HILIC, SEC (GPC-GFC), IEC and ligand exchange chromatography • Dimensions: micro, analytical and preparative columns • CD and RI-detectors • Manufacturer of polymer standards like Pullulan, PMMA and PS • Huge application database 160

Spark Holland BV We are a leading supplier of front-end HPLC and UHPLC instruments such as autosamplers, column ovens and pumps. Our autosamplers have become a benchmark for the industry and we have a reputation as dependable, no-worry OEM partner. Combined with our advanced technology for online Solid Phase Extraction, our instruments enable unsurpassed performance and reliability for automated HPLC and UHPLC based assays.

Tosoh Bioscience GmbH Tosoh Bioscience is a leading manufacturer in the field of bio chromatography. The portfolio encompasses SEC/GPC instruments and a comprehensive line of media and HPLC columns for all common modes of liquid chromatography, including IEX, HIC, mixed-mode, reversed phase, HILIC, SEC and affinity. TOYOPEARL® and TSKgel® resins and columns are renowned for their quality and reliability. Latest product innovations include size exclusion columns tailored to monoclonal antibody analysis and multimodal media for preparative chromatography. We offer solutions for research, drug discovery, clinical chemistry, environmental analysis, manufacturing and other industrial applications. Our technical support team offers application development, on-site training and workshops.

VICI AG International VICI (Valco Instruments Co. Inc. and VICI AG International) is one of the leading sources for top quality components for HPLC, UHPLC, LC, GC, FIA, SFE, SFC and Lab Safety. The Valco, Cheminert, Jour, and Precision Sampling product lines include injectors, selection valves for low pressure, HPLC and UHPLC, valve actuators, polymeric and metal fittings, inline and solvent filters, polymeric tubing (PEEK, PTFE, ETFE, FEP, PFA), metal tubing (Stainless Steel, Titanium, Nickel, Hastelloy C), sample loops, syringes, tools, dilutor/dispensers, liquid handling pumps, safety filters and safety caps for bottles and other canisters, CO2 and humidity filters.

VWR International GmbH VWR enables the advancement of the world's most critical research through the distribution of a highly diversified product line to most of the world's top pharmaceutical and biotech companies, as well as industrial, educational and governmental organizations. With over 150 years of industry experience, VWR offers a well-established distribution network that reaches thousands of specialized labs and facilities spanning the globe. VWR offers a Chromatography portfolio of leading manufacturers plus the VWR Collection brand. You get a choice of quality and price as well as advice from Chromatography specialists, application laboratoratories and Chromatography service technicians.

Wyatt Technology Europe GmbH

YMC Europe GmbH YMC Europe offers a wide range of UHPLC and HPLC columns, bulk silica media for preparative chromatography and lab- and pilot-scale glass columns for customer packing. In addition we provide an on demand service for application support and method development. As a new field of business YMC has strongly invested in the sector of micro reaction technology and continuous synthesis. This new range of products, developed and engineered in YMC facilities, is available worldwide supported by dedicated YMC product specialists. Our extensive distribution network guarantees availability of all YMC products in countries all over the world.

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Exhibition Area

General Floor Plans

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Expo A area (Auditorium Lounge)

Expo U area (Upper Lounge)

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Expo D area (Diamond Lounge) and Expo E area (Europe Foyer)

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Social Events

Welcome Reception Sponsored by Shimadzu 19:10 – 21:00 Sunday, 16 June, 2013 Location: Auditorium Lounge and Upper Lounge Free of charge

Reception Sponsored by Elsevier 18:00 – 19:30 Monday, 17 June, 2013 Location: Café Amsterdam and Europe Foyer Free of charge

Symposium Dinner and Party 19:30 – 23:30 Wednesday, 19 June, 2013

On Wednesday evening the symposium dinner takes place in the Rhone Congres & Party Centre • Rhôneweg 12 - 14 • 1043 AH Amsterdam

The Party Centre can easily be reached by Metro (line 50) from the RAI area or by train from Amsterdam CS to station Amsterdam Sloterdijk. All participants: €25,-

Farewell Reception 17:00 – 18:00 Thursday, 20 June, 2013 Location: First Floor restaurant All attendees are invited for an informal farewell meeting.

We are looking forward to meet you next HPLC2013 symposium to be held in Hobart, Tasmania, Australia

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General Information

Date and Venue The HPLC2013 Symposium is held from 16-20 June, 2013.

Symposium and Exhibition Venue Amsterdam RAI Address: Europaplein, 1078 GZ, Amsterdam, The Netherlands Phone: +31 (0) 20 549 12 12 Fax: +31 (0) 20 646 44 69 Website: http://www.rai.nl/en/ Registration and Information Desk The registration desk is located close to the entrance.

Opening hours 8:30 – 10:00 Sunday, 16 June, 2013 (short-course participants only) 14:30 – 20:30 Sunday, 16 June, 2013 8:00 – 18:00 from Monday, 17 June to Wednesday, 19 June, 2013 8:00 – 15:00 Thursday, 20 June, 2013 Symposium delegates and their companions will receive their badges, symposium materials and social event tickets at the registration desk. Badges You are required to wear your name badge visibly when participating in the conference. Catering Services

Coffee and lunch breaks Coffee, tea, refreshments and lunch (included in the registration fee) will be served in the exhibition and poster area (locations designated with a red-coloured C on the exhibition maps). Internet Corner The internet corner is located in the back of area E (Europe Foyer). Mobile Phones Please switch off your mobile phones during the lecture sessions. Smoking Smoking is not allowed in the congress centre. Wi-Fi There will be free Wi-Fi for all participants and exhibitors in the RAI Conference Centre.

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Public Transportation Travelling within Amsterdam is straight-forward and convenient. There is an extensive transport network connecting the city's many districts, and commuters can choose a variety of methods to reach their destination as efficiently as possible. This includes the train, tram, metro, bus and ferry. The I Amsterdam City Card gives you unlimited use of the GVB public transport system for 24, 48 or 72 hours.

Metro Amsterdam's metro runs underneath the centre, from Central Station to the outer districts. This can be a fast and efficient way to travel, though the trains only run above ground away from the centre, so you lose out on the picturesque view. You'll need a PT Smart Card (OV-chipkaart) to ride the metro. All tickets must be purchased in advance at the GVB Tickets & Info office or GVB Ticket Vending Machines. From Amsterdam Central Station to the RAI Congress Centre: Metro line 51, stop Station Amsterdam RAI.

Tram Trams are common in Amsterdam, with routes heading out from the centre in all directions. Trams are ideal for short journeys around the city centre, as well as for travelling to other districts. You'll need a PT Smart Card (OV- chipkaart) to ride the tram. Only single-use disposable tickets can be purchased from the conductor. Tickets valid for multiple journeys can be purchased at the GVB Tickets & Info office or GVB Ticket Vending Machines.

Bus Amsterdam operates a full bus network, which will take you anywhere you have to go. Just like the tram, only single-journey tickets can be purchased on board. Tickets valid for multiple journeys can be purchased at the GVB Tickets & Info office or GVB Ticket Vending Machines.

Taxi Taxis can be handy when travelling to and from the airport, late at night when the trams have stopped running or when you're running late. Depending on the traffic during peak times, however, public transport may get you there even quicker. All licensed taxi drivers in Amsterdam must adhere to a defined fare structure. Below is an overview of the three components that are used to calculate the total price paid per journey. • Maximum start price: 2.83 euros • Maximum price per kilometre: 2.08 euros • Maximum price per minute: 0.34 euros Amsterdam city centre is a myriad of roads, lanes and footpaths. Because space in the city centre is limited, taxis cannot stop anywhere they like. To keep traffic flowing in the city at peak efficiency, stopping is not allowed at various places.

Planning your journey Before seeing the sights, you may wish to consult the 9292 route planner or GVB website to help you find the quickest and most convenient way to get from A to B. In addition, there are a number of excellent apps available to help you find your way around the city.

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