Acta Scientiarum http://www.uem.br/acta ISSN printed: 1679-9283 ISSN on-line: 1807-863X Doi: 10.4025/actascibiolsci.v38i2.31694 Induction of in vitro shoots of Billbergia euphemiae E. Morren (Bromeliaceae) from leaf explants Mariela Justiniano Simão1*, Daniele Vidal Faria2, Elias Terra Werner3, Taís Cristina Bastos Soares3 and Andreia Barcelos Passos Lima Gontijo4 1Núcleo de Biotecnologia Vegetal, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524 PHLC, 20550-013, Rio de Janeiro, Rio de Janeiro, Brazil. 2Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil. 3Universidade Federal do Espírito Santo, Centro de Ciências Agrárias, Alegre, Espírito Santo, Brazil. 4Universidade Federal do Espírito Santo, Centro Universitário Norte do Espírito Santo, Departamento de Ciências Agrárias e Biológicas, São Mateus, Espírito Santo, Brazil. *Author for correspondence. E-mail:
[email protected] ABSTRACT. Bromeliads are an important group for the maintenance of the Atlantic Forest, with many threatened species due to exacerbated extraction and destruction of their natural habitats. Considering the need of developing protocols for the conservation of these species, the aim of this work was to evaluate the effect of different growth regulators in the in vitro induction of shoots of Billbergia euphemiae. Leaf explants were excised from seedlings derived from in vitro germination and grown on MS medium supplemented with NAA (0, 1 or 2 μM) and BA (0, 2, 4 or 6 μM) combinations. The evaluation of the number of shoots per explant, shoot length, number of leaves per shoot and longest leaf length average was carried out after 30 and 60 days of culture.