Electronic Supplementary Material (ESI) for Analyst This journal is © The Royal Society of Chemistry 2013

Supplemental Table 2A This table includes the taxonomically significant peptides from Vero cell cultured VACV samples purifiedby s\ucrose cusion and one sucrose gradient purified. The peptide, uniprot ID and protein name from one of the homologous proteins is provided. The species specificity is provided along with the frequency of observation (n = 12). Peptides observed in more than replicate data set are included.

Peptide Uniport ID Protein Description Organism Specificity Freq. ISQTYQQQYGR F6TG27 Annexin A4 Primates 0.33 FENAFLSHVISQHQALLG ATP synthase subunit TIR F7ETD0 alpha Primates 0.75 ATP synthase subunit YGLIPEEFFQFLYPK F7EHQ1 beta Primates 0.75 ATP synthase subunit FSPLTTNLINLLAENGR F6S4N7 O, mitochondrial Primates 0.33 Heat shock protein LATQSNEITIPVTFESR F6XTF7 beta-1 Primates 0.83 Cytochrome b-c1 complex subunit 2, TIAQGNLSNTDVQAAK P22695 mitochondrial Primates and Pig 0.50 GGPGSAVSPYPTFNPSSD All Primates plus VAALHK F7D2H1 Annexin A1 elephant 0.33 Malate dehydrogenase All Primates scattered VDFPQDQLTALTGR F6YCN0 (Fragment) mammals 0.33 Heat shock protein All Primates, plus VSLDVNHFAPDELTVK F6XTF7 beta-1 mouse 0.17 ATP synthase subunit LAALPENPPAIDWAYYK F7HS39 d, mitochondrial Most Primates 0.33 FFESFGDLSSADAILGNP Hemoglobin fetal K P02081 subunit beta Bos taurus 0.08 AVEHLDDLPGALSELSDL Hemoglobin subunit HAHK P01966 alpha Bos taurus 0.08 Hemoglobin subunit VGGHAAEYGAEALER P01966 alpha Bos taurus 0.33 Electronic Supplementary Material (ESI) for Analyst This journal is © The Royal Society of Chemistry 2013

Complement component 1 Q subcomponent- LHTEGDKAFVDFLNDEIK binding protein, African Green EER Q9MZE0 mitochondrial Monkey 0.33

TIGTGLVTDTPTMTEEDK F7E244 Elongation factor Tu Horse 0.42 ATP synthase subunit ELAEAQDDSILK Q06185 e, mitochondrial Mouse 0.08 ITKFENAFLSHVISQHQA ATP synthase subunit Primates, no great LLGTIR F7ETD0 alpha apes 0.17 Superoxide dismutase Primates, no great GDSPVQGTINFEQK Q8HXQ0 [Cu-Zn] apes 0.17

39S ribosomal protein Primates, no great LTEANPVDKVK F7H0R4 L12, mitochondrial apes 0.17 Hydroxyacyl- TLSSIATSTDAASVVHST coenzyme A Primates, no great DLVVEAIVENLK F7DIX8 dehydrogenase apes 0.83 GILGYTEHQVVSSDFNSD Glyceraldehyde-3- THSSTFDAGAGIALNDHF phosphate VK F6ZXW5 dehydrogenase Some Primates 0.25 ATP synthase subunit VAASVLNPYVK F6S4N7 O, mitochondrial Primates 0.33 Cytochrome c oxidase GINTLVTYDLVPEPK Q53CF8 subunit 5A Some Primates 0.25 LPEEWSQWLGGSSWPGY VRPLPPAAIESPAVAAPA Heat shock protein YSR F6XTF7 beta-1 Some Primates0.17 Mitochondrial inner TSSAETPTIPLGSAVEAIK F7A1P8 membrane protein Some Primates 0.67 Leucine-rich PPR LANQFAIYKPVTDFFLQL motif-containing VDAGKVDDAR F7FU95 protein, mitochondrial Some Primates 0.17 Superoxide dismutase HVGDLGNVTAGK Q8HXQ0 [Cu-Zn] Macaque, Pig, Mouse 0.58