DOCSLIB.ORG

University of Florida Thesis Or Dissertation Formatting

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
University of Florida Thesis Or Dissertation Formatting THE DEVELOPMENT OF AN EXTRACELLULAR VESICLE ISOLATION PROTOCOL FOR GINGIVAL CREVICULAR FLUID By ESTELA L. TRUZMAN A THESIS PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE UNIVERSITY OF FLORIDA 2019 © 2019 Estela L. Truzman To my family and my husband for their infinite support, inspiration and love ACKNOWLEDGMENTS I would like to thank my mentors Dr. Shannon Holliday and Dr. Wellington Rody for their guidance and support throughout this process. I would also like to thank the faculty, staff and residents at the University of Florida’s Department of Orthodontics for their unwavering support throughout these past 3 years. Last, but not least, I would like to thank my wonderful parents and husband for always encouraging me to pursue my dreams. 4 TABLE OF CONTENTS page ACKNOWLEDGMENTS .................................................................................................. 4 LIST OF TABLES ............................................................................................................ 7 LIST OF FIGURES .......................................................................................................... 8 LIST OF ABBREVIATIONS ........................................................................................... 10 ABSTRACT ................................................................................................................... 11 CHAPTER 1 INTRODUCTION .................................................................................................... 13 Background ............................................................................................................. 13 Biomarkers in Orthodontics ..................................................................................... 16 Osteoclastogenesis Related Factors (RANKL/OPG) ........................................ 16 Biomarkers of Inflammation .............................................................................. 17 Extracellular Matrix Degradation Factors (MMPs) ............................................ 17 Dentin Breakdown Products ............................................................................. 18 Extracellular Vesicles (EVs) ............................................................................. 20 Biomarkers in Oral Fluids ........................................................................................ 26 Saliva................................................................................................................ 26 Gingival Crevicular Fluid (GCF) ........................................................................ 27 GCF Collection ....................................................................................................... 27 Gingival Washing Method................................................................................. 28 Capillary Tubing or Micropipettes ..................................................................... 28 Absorbent Filter Paper Strips ........................................................................... 29 Durapore Filter Membranes .............................................................................. 30 Specific Objectives or Hypotheses ......................................................................... 31 Objective .......................................................................................................... 31 Hypotheses ...................................................................................................... 31 2 MATERIALS AND METHODS ................................................................................ 33 Participants, Eligibility Criteria ................................................................................. 33 Study Design .......................................................................................................... 33 Sample Collection ................................................................................................... 33 PerioPaper Strip ............................................................................................... 34 Durapore Filter Membrane ............................................................................... 34 Microcapillary Tube .......................................................................................... 34 Control Samples Collection .............................................................................. 35 Sample Preparation ................................................................................................ 35 EV Isolation with Nanoparticle Tracking Analysis (NTA) ......................................... 35 5 Statistical Analysis .................................................................................................. 36 3 RESULTS ............................................................................................................... 41 4 DISCUSSION ......................................................................................................... 51 Main Findings ......................................................................................................... 51 Limitations ............................................................................................................... 55 5 CONCLUSIONS ..................................................................................................... 57 LIST OF REFERENCES ............................................................................................... 58 BIOGRAPHICAL SKETCH ............................................................................................ 63 6 LIST OF TABLES Table page 2-1 Outline of Inclusion and Exclusion Criteria ......................................................... 37 3-1 Raw Data with Average Concentration Obtained from the NanoSight Software (version 2.3, NanoSight) ...................................................................... 44 3-2 Volume of GCF Collected with PerioPaper and Average Concentration of Nanoparticles ...................................................................................................... 45 3-3 Average Nanoparticle Concentration of Control Samples .................................. 45 3-4 Comparison of Raw Data to Control Values ....................................................... 45 3-5 Adjusted Data- Average Control Values Subtracted from the Raw Data ............ 46 3-6 Concentration Difference between the three Collection Methods ....................... 46 3-7 Correlation between the adjusted values of the three methods .......................... 46 7 LIST OF FIGURES Figure page 1-1 Size distribution and concentration of nanoparticles recovered from control and GCF samples. Analysis performed on the NanoSight instrument available at UF-ICBR .......................................................................................... 32 2-1 PerioPaper, 2018. Courtesy of Dr. Estela Truzman. PerioPaper collection method on tooth #8 ............................................................................................. 38 2-2 Periotron 8000®, 2018. Courtesy of Dr. Estela Truzman GCF volume determination with the Periotron 8000® (Oraflow, Plainview, NY, USA) ............ 38 2-3 Durapore, 2018. Courtesy of Dr. Estela Truzman Volume of GCF collected with PerioPaper and concentration of nanoparticles ........................................... 38 2-4 Microcapillary tube, 2018. Courtesy of Dr. Estela Truzman Microcapillary tube collection method on tooth #8 ..................................................................... 39 2-5 Leuer port, 2018. Courtesy of Dr. Estela Truzman Installation of the leuer port on the NanoSight NS300 ............................................................................. 39 2-6 Leuer Port Connection, 2018. Courtesy of Dr. Estela Truzman Connection of the leuer port into the top-plate of the NanoSight NS300. .................................. 40 2-7 Top-plate, 2018. Courtesy of Dr. Estela Truzman 1 ml disposable syringe connected to a luer port on the top-plate of the NanoSight NS300..................... 40 3-1 Bar graph with raw data obtained from the NanoSight software. The average concentration of each method including the Standard deviation are shown ....... 47 3-2 Graph from the NanoSight showing the average nanoparticle concentration of a PerioPaper sample ...................................................................................... 47 3-3 Graph from the NanoSight showing the average nanoparticle concentration of a Durapore membrane sample ....................................................................... 48 3-4 Graph from the NanoSight showing the average nanoparticle concentration of a micropipette ................................................................................................. 48 3-5 Bar graph with adjusted data. The average control values were subtracted from the raw data for each sample. This number was multiplied by 15.7, which was the dilution media .............................................................................. 49 3-6 PerioPaper vs. Durapore Spearman correlation coefficient ................................ 49 3-7 PerioPaper vs. micropipette Spearman correlation coefficient ........................... 50 8 3-8 Durapore vs. micropipette Spearman correlation coefficient .............................. 50 9 LIST OF ABBREVIATIONS CBCT Cone Beam Computed Tomography CT Scan Computerized Tomography Scan DMP-1
Load more

Recommended publications
  • The Art and Science of Shade Matching in Esthetic Implant Dentistry, 275 Chapter 12 Treatment Complications in the Esthetic Zone, 301
    FUNDAMENTALS OF ESTHETIC IMPLANT DENTISTRY Abd El Salam El Askary FUNDAMENTALS OF ESTHETIC IMPLANT DENTISTRY FUNDAMENTALS OF ESTHETIC IMPLANT DENTISTRY Abd El Salam El Askary Dr. Abd El Salam El Askary maintains a private practice special- Set in 9.5/12.5 pt Palatino izing in esthetic dentistry in his native Egypt. An experienced cli- by SNP Best-set Typesetter Ltd., Hong Kong nician and researcher, he is also very active on the international Printed and bound by C.O.S. Printers Pte. Ltd. conference circuit and as a lecturer on continuing professional development courses. He also holds the position of Associate For further information on Clinical Professor at the University of Florida, Jacksonville. Blackwell Publishing, visit our website: www.blackwellpublishing.com © 2007 by Blackwell Munksgaard, a Blackwell Publishing Company Disclaimer The contents of this work are intended to further general scientific Editorial Offices: research, understanding, and discussion only and are not intended Blackwell Publishing Professional, and should not be relied upon as recommending or promoting a 2121 State Avenue, Ames, Iowa 50014-8300, USA specific method, diagnosis, or treatment by practitioners for any Tel: +1 515 292 0140 particular patient. The publisher and the editor make no represen- 9600 Garsington Road, Oxford OX4 2DQ tations or warranties with respect to the accuracy or completeness Tel: 01865 776868 of the contents of this work and specifically disclaim all warranties, Blackwell Publishing Asia Pty Ltd, including without limitation any implied
    [Show full text]
  • Mmubn000001 232074720.Pdf
    PDF hosted at the Radboud Repository of the Radboud University Nijmegen The following full text is a publisher's version. For additional information about this publication click this link. http://hdl.handle.net/2066/146250 Please be advised that this information was generated on 2021-10-05 and may be subject to change. ORTHODONTIC FORCES AND TOOTH MOVEMENT J .J . G . M . Pilon Omslag: Clemens Briels / beeldend kunstenaar Acryl op linnen Formaat: 30 χ 40 cm ORTHODONTIC FORCES AND TOOTH MOVEMENT An experimental study in beagle dogs ISBN 90-9009878-Х ORTHODONTIC FORCES AND TOOTH MOVEMENT An experimental study in beagle dogs Een wetenschappelijke proeve op het gebied van de Medische Wetenschappen. PROEFSCHRIFT ter verkrijging van de graad van doctor aan de Katholieke Universiteit Nijmegen, volgens besluit van het College van Decanen in het openbaar te verdedigen op dinsdag 1 oktober 1996 des namiddags om 3.30 uur precies door Johannes Jacobus Gertrudis María Pilon geboren 12 juni 1959 te Geleen 1996 Druk: ICG printing b.v., Dordrecht Promotor Prof.dr. A.M. Kuijpers-Jagtman Copromotor Dr. J.C. Maltha Manuscriptcommissie Prof.dr. H.H. Renggli Prof.dr. N.H.J. Creugers Prof.dr. R. Huiskes Deze studie werd verricht bij de vakgroep Orthodontie en Orale Biologie (hoofd: Prof.dr. A.M. Kuijpers-Jagtman) van de Katholieke Universiteit Nijmegen. Dit onderzoek was onderdeel van hoofdprogramma VI "Orale Aandoeningen en Steunweefselziekten". Contents Chapter 1 General introduction 13 Chapter 2 Force degradation of orthodontic elastics 23 Submitted to the European Journal of Orthodontics, 1996. Chapter 3 Spontaneous tooth movement following extraction of mandibular third premolars in beagle dogs 39 Chapter 4 Magnitude of orthodontic forces and rate of bodily tooth movement, an experimental study in beagle dogs 49 Published in the American Journal of Orthodontics and Dentofacial Orthopedics (1996) 110: 16-23.
    [Show full text]
  • Periodontal Considerations in Orthodontic Treatment
    Review Article DOI: 10.18231/2455-6785.2017.0004 Periodontal considerations in orthodontic treatment Vasu Kumar1,*, Vijayta Yadav2, Mani Dwivedi3, Kusum Lata Agarwal4, Saquib Ahmad Asrar5 1,3,4,5PG Student, 2Senior Lecturer, Dept. of Orthodontics, Career PG Institute of Dental Sciences, Uttar Pradesh *Corresponding Author: Email: [email protected] Abstract Harmonious cooperation of the general dentist, the periodontist and the orthodontist offers great possibilities for the treatment of combined orthodontic–periodontal problems. Orthodontic treatment along with patient’s compliance and absence of periodontal inflammation can provide satisfactory results without causing irreversible damage to periodontal tissues. Orthodontic treatment carries with it the risks of tissue damage, treatment failure and an increased predisposition to dental disorders. The dentist must be aware of these risks in order to help the patient make a fully informed choice whether to proceed with orthodontic treatment. The aim of this study is to discuss the principles of orthodontic treatment in patients with reduced periodontium, its indications and limitations, as well as current views concerning retention of orthodontic result. Keywords: Periodontal tissues, Retention, Periodontium, Orthodontics Introduction a. The occurrence of any previous periodontal disease Certain malocclusion traits are associated with b. Drug history, e.g. use of long-term corticosteroids, difficulties in maintaining good oral hygiene and as a phenytoin, etc. consequence to poor periodontal condition.(1) c. Systemic diseases or physiological conditions, e.g. Therefore, proper alignment of the teeth provided by pregnancy, diabetes, asthma, chronic renal failure, orthodontic treatment may promote good control of soft etc. deposit and calculus and subsequent periodontal inflammation. It has been known that orthodontic Clinical Examination: appliances have been a local etiologic factor Check for the following: contributing to periodontal problems.
    [Show full text]
  • Pragmatic Trial Design Considerations: the PRECIS-2 Framework
    Pragmatic Trial Design Considerations: The PRECIS-2 Framework Elaine H. Morrato, DrPH MPH Associate Professor, Health Systems, Management and Policy Associate Dean for Public Health Practice Director, Pragmatic Trials and Dissemination-Implementation Research, Colorado Clinical & Translational Sciences Institute Duke Industry Statistics Symposium 2017 | Are Pragmatic Clinical Trials Ready for Prime Time? September 7, 2017 1 Objectives • Review PRECIS-2, a framework for pragmatic trial design & reporting • Apply the PRECIS-2 framework to an example of designing a Phase IIIb drug trial 2 A randomized controlled trial to inform decisions about practice & real-world effectiveness PRAGMATIC TRIALS Review Article The Changing Face of Clinical Trials No clinical trial is completely explanatory or pragmatic. Trials exist on a continuum. Efficacy Effectiveness Explanatory Trial Pragmatic Trial Can an intervention work Does the intervention work under ideal conditions? under real-world conditions? 4 PRagmatic-Explanatory Continuum Indicator Summary (PRECIS-2) 1 = very explanatory 5 = very pragmatic https://crs.dundee.ac.uk/precis BMJ 2015;350:h2147 5 Case Application: Effectiveness of site-specific antibiotic treatment for periodontal disease Actisite® (tetracycline periodontal) periodontal fiber 6 7 Good pragmatic trial research requires stakeholder engagement • To produce information that is meaningful and useful in practice, we must understand priorities and needs from the perspective of patients and other stakeholders • Patient- and stakeholder-centered
    [Show full text]
  • Tissue Response to Orthodontic Force in Beta-Aminopropionitrile Treated Rats" (1978)
    University of Connecticut OpenCommons@UConn SoDM Masters Theses School of Dental Medicine June 1978 Tissue Response to Orthodontic Force in Beta- Aminopropionitrile Treated Rats Ira J. Heller Follow this and additional works at: https://opencommons.uconn.edu/sodm_masters Recommended Citation Heller, Ira J., "Tissue Response to Orthodontic Force in Beta-Aminopropionitrile Treated Rats" (1978). SoDM Masters Theses. 125. https://opencommons.uconn.edu/sodm_masters/125 TISSUE RESPONSE TO ORTHODONTIC FORCE IN BETA-&~INOPROPIONITRILE TREATED RATS BY Ira J. Heller, B.S., D.D.S. Submitted in Partial Fulfill~ent of the Requirem~nt for a Certificat~ in Orthodontics June 15, 1978 Department of Orthodontics School of Dental Medicine University of Connecticut Health Center Farmington, Connecticut 06032 ACI010WLEDGEMENT I wish to thank Dr. Ravindra Nanda fo::: ·P.is guidance, support, and advice during all phases of this in­ vestigation. The technical assistance of Mrs. S~an Cowan is grate­ fully ackno~yledged. This investigation was supported by a grant from the Connecticut Research Foundation. i i TABLE OF CONTENTS Introduction •••Page 1 Review of the Literature ••••••••••••••••••••••Page 3 Materials and Methods ••Page 8 Results ••••••••Page 14 Discussion •••••••Page 20 Summary and Conclusions ••••••••••••••••Page 25 References ••••••••••••••••Page 27 Tables ••Page 31 Figures .Page 35 Appendix •••••••••••••••.••••••••••••••••••••••Page 48 i i i List of Tab:es Table I- Summary of Method of ~d~inistratio~ of Lat11yrogens. •...••..•• .•.•. •••••• l'age 31 Table II - Summary of Animal \·leight and Food Consumption Data...... •• .. •••.•••• .. •..• .. •.••••.• •. Page 32 Table III - Statistical Evaluation 0: Alveolar Bone Response at Mesial ~oot Alveolus. •.• • • . • . .. .. .. .....•.•. .. .• .. .. ..........• Page 33 Table IV - Statistical Evaluation of AlveJlar Bone Response at Inter1e~tal Septum••.•.•• ~ .
    [Show full text]
  • Contents Focus on Dentistry September 18-20, 2011 Albuquerque, New Mexico
    Contents Focus on Dentistry September 18-20, 2011 Albuquerque, New Mexico Thanks to sponsors Boehringer Ingelheim Vetmedica, Equine Specialties, Pfizer Animal Health, and Capps Manufacturing, Inc. for supporting the 2011 Focus on Dentistry Meeting. Sunday, September 18 Peridental Anatomy: Sinuses and Mastication Muscles ............................................... 1 Victor S. Cox, DVM, PhD Dental Anatomy .................................................................................................................8 P. M. Dixon, MVB, PhD, MRCVS Equine Periodontal Anatomy..........................................................................................25 Carsten Staszyk, Apl. Prof., Dr. med. vet. Oral and Dental Examination .........................................................................................28 Jack Easley, DVM, MS, Diplomate ABVP (Equine) How to Document a Dental Examination and Procedure Using a Dental Chart .......35 Stephen S. Galloway, DVM, FAVD Equine Dental Radiography............................................................................................50 Robert M. Baratt, DVM, MS, FAVD Beyond Radiographs: Advanced Imaging of Equine Dental Pathology .....................70 Jennifer E. Rawlinson, DVM, Diplomate American Veterinary Dental College Addressing Pain: Regional Nerve Blocks ......................................................................74 Jennifer E. Rawlinson, DVM, Diplomate American Veterinary Dental College Infraorbital Nerve Block Within the Pterygopalatine Fossa - EFBI-Technique
    [Show full text]
  • Synthesis of Sharpey's Fiber Proteins Within Rodent Alveolar Bone
    Scanning Microscopy Vol. 12, No. 2, 1998 (Pages 317-327) 0891-7035/98$5.00+.25 Scanning Microscopy International, Chicago (AMFSynthesis O’Hare), of IL Sharpey’s 60666 USA fibers SYNTHESIS OF SHARPEY’S FIBER PROTEINS WITHIN RODENT ALVEOLAR BONE R.B. Johnson1* and R.H. Martinez2 1Dept. of Diagnostic Sciences, Univ. of Mississippi School of Dentistry, 2500 North State Street, Jackson, Mississippi 39216-4505; 2Departmento de Ortodoncia, Escuela Colombiana de Medicina, Bogotá, Colombia (Received for publication April 29, 1996 and in revised form September 24, 1996) Abstract Introduction: Review of the Literature Physiologic drift of teeth requires remodeling alve- Sharpey’s fibers attach tendons and ligaments to olar bone at the tooth socket wall. Remodeling must be bone. They consist of a bundle of collagen fibers and are regulated so that collagenous attachments are maintained usually partially mineralized (Selvig, 1965; Boyde and Jones, as new tooth positions are established. Thus, alveolar 1968; Shackleford, 1973; Jones and Boyde, 1974; Johnson, remodeling must occur coincident to remodeling of the 1983). The fibers are surrounded by a mineralized, Sharpey’s fibers which are embedded within that bone. collagenous sheath (Johnson, 1983). Studies suggest that these fibers are severed at resorptive For many years, it was presumed that Sharpey’s sites on the alveolar wall and continuity with periodontal fibers were relatively inert. However, recent studies suggest ligament fibers is reestablished by splicing, de novo that they readily adapt to stress/strain forces coincident to synthesis, or adhesion to the base of the Howship’s lacuna functional movements of adjacent teeth. The site of at the alveolar wall; however, little information is available adaptation is at the periosteal surface of bone, where the concerning the mechanisms of these events.
    [Show full text]
  • Parameters of the Attached Epithelial Reformation Following Gingivectomy
    Loyola University Chicago Loyola eCommons Master's Theses Theses and Dissertations 1978 Parameters of the Attached Epithelial Reformation Following Gingivectomy Carl J. Laudando Loyola University Chicago Follow this and additional works at: https://ecommons.luc.edu/luc_theses Part of the Oral Biology and Oral Pathology Commons Recommended Citation Laudando, Carl J., "Parameters of the Attached Epithelial Reformation Following Gingivectomy" (1978). Master's Theses. 2983. https://ecommons.luc.edu/luc_theses/2983 This Thesis is brought to you for free and open access by the Theses and Dissertations at Loyola eCommons. It has been accepted for inclusion in Master's Theses by an authorized administrator of Loyola eCommons. For more information, please contact [email protected]. This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License. Copyright © 1978 Carl J. Laudando PARAMETERS OF THE ATTAOffiD EPITHFLIAl REFO~~TION FOLLOWING GINGIVECT01'~1Y By Carl J. Laudando PARAMETERS OF TI-!F ATTArnED EPITHELIAL REFO~~TION FOLL01VING GINGIVECTOJvfY By Carl J. Laudando, B.S., D.D.S. A Thesis Submitted to the Faculty of the Graduate School of Loyola University in Partial Fulfillment of the Requirements for the Degree of ~/faster of Science in Oral Biology May 1978 T ~·' • DEDICATION To my mother and father, Ignatius and Rose Laudando, for their never ending encouragement, love and understanding. To my wife, Nancy, for her love, sacrifice, and patience. ] ACKNOWLFDGJvlF.t,rrs I would like to express my appreciation to the members of my advisory committee: Doctor Lawrence Jenkins, Doctor Anthony W. Gargiulo, Doctor Patrick Toto, Doctor Hal McReynolds, and Doctor Daniel Grant for their assistance and recommendations during the research and writting of this thesis.
    [Show full text]
  • Hyaluronan-CD44 Interactions Mediate Contractility and Migration in Periodontal Ligament Cells
    Cell Adhesion & Migration ISSN: 1933-6918 (Print) 1933-6926 (Online) Journal homepage: https://www.tandfonline.com/loi/kcam20 Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells Zeinab Al-Rekabi, Adriane M. Fura, Ilsa Juhlin, Alaa Yassin, Tracy E. Popowics & Nathan J. Sniadecki To cite this article: Zeinab Al-Rekabi, Adriane M. Fura, Ilsa Juhlin, Alaa Yassin, Tracy E. Popowics & Nathan J. Sniadecki (2019) Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells, Cell Adhesion & Migration, 13:1, 139-151, DOI: 10.1080/19336918.2019.1568140 To link to this article: https://doi.org/10.1080/19336918.2019.1568140 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. View supplementary material Accepted author version posted online: 24 Jan 2019. Published online: 08 Feb 2019. Submit your article to this journal Article views: 534 View Crossmark data Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=kcam20 CELL ADHESION & MIGRATION 2019, VOL. 13, NO. 1, 139–151 https://doi.org/10.1080/19336918.2019.1568140 RESEARCH ARTICLE Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells Zeinab Al-Rekabia, Adriane M. Furab, Ilsa Juhlina, Alaa Yassinc, Tracy E. Popowicsd, and Nathan J. Sniadecki a,b,e aDepartment of Mechanical Engineering, University of Washington, Seattle, WA, USA; bDepartment of Bioengineering, University of Washington, Seattle, WA, USA; cDepartment of Periodontics, University of Washington, Seattle, WA, USA; dDepartment of Oral Health Sciences, University of Washington, Seattle, WA, USA; eInstitute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA ABSTRACT ARTICLE HISTORY The role of hyaluronan (HA) in periodontal healing has been speculated via its interaction with the Received 8 August 2018 CD44 receptor.
    [Show full text]
  • Cementum.Pdf
    DR JEBIN,MDS.,D.ICOI The periodontium is defined as those tissues supporting and investing the tooth. • It consists of the cementum, • the periodontal ligament, • the alveolar bone and •the gingiva. Cementum is the least understood one of these four tissues. • The Cementum is a hard, avascular, calcified connective tissue covering the root dentin and gives insertion to the periodontal fiber bundle. • It can be regarded as a “bone of attachment”. • It is pale yellow and softer than dentin. • Cementum is formed throughout life and is resistant to resorption. • Cementum functions as an area of attachment for the periodontal ligament fibers. Thickness of cementum: • The thickness of cementum varies considerably coronal third may be 16-60 µm thick (thickness of hair) apical third and furcation areas can be 150-200 µm or even thicker (greatest thickness) • It is thicker in distal surfaces than in mesial surfaces. • Between 11 and 70 years, the average thickness of cementum increases threefold, with greatest increase in apical region. • Average thickness: 95 µm at 20yrs and 215 µm at 60yrs Permanent teeth contains • 45-50% inorganic substance • 50-55% organic material and water. • The inorganic portion consists calcium and phosphate in the form of hydroxyapatite (Ca10[Po4]6[OH]2) • They are thin, needle-shaped crystals. • It is less than that of bone(65%) enamel(97%) dentin(70%) a • The organic portion of the cementum is composed primarily of type I (90%) and type III (about 5%) collagens. • Sharpey’s fibers, which constitute the major portion of cementum are composed of mainly collagen type I.
    [Show full text]
  • Doconut Mose
    DOCONUT MOSE ED 183 960 CB 024 027 .TITLE Military Curricula for Vocational.8 Technical Education. Dental Specialist, 10-1. INSTITUTION ,kir Univ., Gunter AFS,Ala.s.Extension Course Inst.: Ohio State_Univ., Columbus. National Center for Research in Vocational Education. SPONS AGENCY Bureau'of Occupational and Adult Education (DHEW/OE), Washington, D.C. P313 DATE -r74]- NOTE 434p.;' Not available in paper copy due to small, light type. EDRS PRICE MF01 Plus Postage. P: Not Available from EDRS. DESCRIPTORS *Allied Health'Occupations Education; Behavioral Objectives:. *Dental Assistants; *Dentistry; High Schools; Postsecondary Education: *Vocational Education IDENTIFIERS Dental Equipment: Dental Therapy Assistants; Military Zurricului Project ABSTRACT Designed to provide entry level apprentice skills to persons who wish to become skilled, specialist level workers as dental assistants, these course materials supplement laboratory or on-the-job learning situations. One of a number of military-developed curriculum packages selected for adaptation to civilian vocational curriculum and instruction, the course contains a considerable aiount of review material and is stated to be suitable for beginning students with background in the basic sciences. Each chapter in the four-volume set is organized around learning objectives, accompanied by text with criterion exercises and answers keyed to the text for self-evaluation. Each volume also has a review examination containing questions keyed to the objectives: no answers are provided. Volaie I, Administration, Safety, and Maintenance, discusses the professional-client relationship and general safety practices. Volume 2, Basic Dental Sciences, covers oral anatomy, physiology, and pathology; dental therapeutics: and basic microbiology and sterilization. Volume 3, Dental Instruments and Materials, describes the uses of general, restorative, and specialty instruments; and dental materials.
    [Show full text]
  • Normal Formation and Development Defects of the Human Dentition
    PEDIATRIC ORAL HEALTH 0031-3955/00 $15.00 + .OO NORMAL FORMATION AND DEVELOPMENT DEFECTS OF THE HUMAN DENTITION J. Tim Wright, DDS, MS The development of the human dentition involves a highly orches- trated series of events that are strictly genetically controlled. The devel- opmental timing, location, morphology, structure, and composition of teeth are primarily determined by cascades of molecular events that are regulated by hundreds of genes.4oNormally, humans have 20 primary (i.e., deciduous, or shedding) teeth, which are lost in childhood, and 32 permanent teeth. Dentition begins to form at approximately 6 weeks in utero and continues through late adolescence, when the development of the permanent third molars is completed. Because the development of dentition is prolonged, it is susceptible to environmental influences for many years. A basic understanding of normal dentition and its development allows clinicians to accurately identdy normal and abnor- mal dental conditions and make recommendations for appropriate thera- peutic interventions and patient counseling. Delineating normal from abnormal dental development requires careful evaluation of the patient, including a medical, dental, and family history; clinical examination; and radiographic evaluation and may require special laboratory tests. This article reviews normal dentition and fundamental concepts of tooth development and provides a conceptual framework for diagnosing de- velopmental defects of teeth. From the Department of Pediatric Dentistry, School of Dentistry, The University of North Carolina, Chapel Hill, North Carolina b PEDIATRIC CLINICS OF NORTH AMERICA VOLUME 47 * NUMBER 5 OCTOBER ZOO0 975 976 WRIGHT TOOTH DEVELOPMENT Early embryonic requisites for tooth development include the differ- entiation of the oral ectoderm and the migration of neural crest cells into the craniofacial region, where tooth buds form.
    [Show full text]