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Effect of Cortisone on the Periodontal Ligament of the Maxillary Rat Incisor

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Effect of Cortisone on the Periodontal Ligament of the Maxillary Rat Incisor Loyola University Chicago Loyola eCommons Master's Theses Theses and Dissertations 1974 Effect of Cortisone on the Periodontal Ligament of the Maxillary Rat Incisor Donald Ralph DeMatteis Loyola University Chicago Follow this and additional works at: https://ecommons.luc.edu/luc_theses Part of the Orthodontics and Orthodontology Commons Recommended Citation DeMatteis, Donald Ralph, "Effect of Cortisone on the Periodontal Ligament of the Maxillary Rat Incisor" (1974). Master's Theses. 2751. https://ecommons.luc.edu/luc_theses/2751 This Thesis is brought to you for free and open access by the Theses and Dissertations at Loyola eCommons. It has been accepted for inclusion in Master's Theses by an authorized administrator of Loyola eCommons. For more information, please contact [email protected]. This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License. Copyright © 1974 Donald Ralph DeMatteis ... EFFECT OF CORTISONE ON THE PERIODONTAL LIGAMENT OF THE MAXILLARY RAT INCISOR by Donald Ralph DeMatteis, D.D.S. A THESIS SUBMITTED TO THE FACULTY OF THE GRADUATE SCHOOL OF LOYOLA UNIVERSITY IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE June 1974 library - Loyola Univc;d; Medical Center LIFE Donald Ralph DeMatteis was born on August 23, 1943, in Auburn, Washington. He was graduated from O'Dea High School, Seattle, Wash­ ington, in June, 1961. He then attended Seattle University in Seattle from 1961 to 1966, graduating with a Bachelor of Science. His undergraduate dental education was obtained from Loyola University, School of Dentistry, Maywood, Illinois. A degree of Doctor of Dental Surgery was awarded him in June, 1970. In June, 1972, he began his graduate studies in the De­ partment of Oral Biology, and postgraduate studies in the Depart­ ment of Orthodontics, Loyola University, Maywood, Illinois. 11 ACKNOWLEDGEMENTS The author wishes to express his sincerest appreciation and gratitude to the following peoplea Michael L. Kiely, Ph.D., who suggested this investigation and for his assistance and time spent on my behalf throughout the extent of my Graduate and Research Training. His efforts were paramount for the completion of this project. Priscilla c. Bourgault, Ph.D. and Wayne w. Beach, D.D.s., for their time spent in this investigation. Danute Augius for her aid in histological sectioning and Valerie Lescinskas for her assistance in radioautographic technique and special staining procedures. Donald c. Hilgers, D.D.s., Ph.D., for his confidence in accepting me into the two year Orthodontic Program leading to a Certificate in Orthodontics. To my wife, Cathy, for her diligent preparation of the manuscripts and for her love, patience and understanding through­ out the many years of my professional training. iii TABLE OF CONTENTS Page I. INTRODUCTION. • • • • • • • • • • • • • • • • • • 1 II. REVIEW OF LITERATURE. • • • • • • • • • • • • • • J A, Experiments Concerned with the Mechanism of Tooth Eruption. • • • • • • • • J B. Effects of Cortisone on the Growth of Oral Tissues •• • • • • • • • • • 8 c. Dynamics of the Periodontal Ligament • • • • • 1) III. MATERIALS AND METHODS • • • • • • • • • • • • • • •• 21 IV. RESULTS • • • • • • • • • • • • • • • • • • • • • • • 2.5 A. Radioautographic Observations •• • • • • • • • 2.5 B. Histologic Observations •• • • • • • • • • • • 26 v. DISCUSSION. • • • • • • • • • • • • • • • • • • • • • 29 VI. SUMMARY AND CONCLUSIONS • • • • • • • • • • • • • • • J9 VII. BIBLIOGRAPHY. • • • • • • • • • • • • • • • • • • • • 42 VIII. TABLES. • • • • • • • • • • • • • • • • • • • • • • • 47 IX. FIGURES AND PLATES. • • • • • • • • • • • • • • • • .56 iv LIST OF TABLES Table Page I AVERAGE NUf;IBER OF 3H-THYMIDINE LABELLED FIBROBLASTS PER UNIT LENGTH OF PERIODONTAL LIGAMENT IN THE MAXILLARY INCISOR OF EXPERIMENTAL AND CONTROL RATS ••••••• • • • • • 47 II ONE WAY ANALYSIS OF VARIANCE (F) TEST FOR DATA SHOWN IN TABLE I - 7 A.M. PERIOD. • • • • • 48 III ONE WAY ANALYSIS OF VARIANCE (F) TEST FOR DATA SHOWN IN TABLE I - 1 P.M. PERIOD. • • • • • 49 IV ONE WAY ANALYSIS OF VARIANCE (F) TEST FOR DATA SHOWN IN TABLE I - 7 P.M. PERIOD. • • • • • 50 V ONE WAY ANALYSIS OF VARIANCE (F) TEST FOR DATA SHOWN IN TABLE I - 1 A.M. PERIOD. • • • • • .51 VI ONE WAY ANALYSIS OF VARIANCE (F) TEST FOR DATA SHOWN IN TABLE I - DETERMINATION OF POSSIBLE CIRCADIAN RHYTHM EFFECT ••••• • • • • 52 VII NUMBER OF 3H-THYMIDINE LABELLED FIBROBLASTS PER UNIT LENGTH (1800 u) OF RAT INCISOR PERIODONTAL LIGAMENT - ADRENALECTOMIZED PLUS CORTISONE • • • • • • • • • • • • • • • • • • • 53 VIII NUMBER OF 3H-THYMIDINE LABELLED FIBROBLASTS PER UNIT LENGTH (1800 u) OF RAT INCISOR PERIODONTAL LIGAMENT - ADRENALECTOMIZED. • • • • • • IX NUMBER OF 3H-THYMIDINE LABELLED FIBROBLASTS PER UNIT LENGTH (1800 u) OF RAT INCISOR PERIODONTAL LIGAMENT - CONTROLS. • • • • • • • • • • 55 v LIST OF FIGURES Figure Page 1 Low power view showing the area of the rat maxillary incisor periodontal ligament in which cell counts were taken (X4o) ••••••••• 56 2 High power view showing "clustering" of JH-thymidine labelled fibroblasts in the periodontium, Note the black silver grains above the nuclei (X450) • • • • • • • • • • • • •• 56 J High power view showing the abundant vascularity and tissue spaces in the periodontium apical to the area of initial predentin formation (X450) ••• 57 4 High power view showing the collagen fibers in the three zones of the periodontal ligament of a control animal (Mallory's aniline blue stain. X450) •••••••••••••••••••• 57 5 High power view of the periodontal ligament of a untreated adrenalectomized animal. Note slight edema and a decrease in collagen fiber density as compared to Figure 4. Direction of fibers is unaltered (Mallory's aniline blue stain. X450) ••••••••••••• 58 6 High power view of the periodontal lig~~ent of a cortisone treated adrenalectomized animal, Note the clumped and homogeneous appearance of the fibers of the middle zone and an alteration in fiber direction of the dental zone as compared to Figures 4 and 5 (Mallory's aniline blue stain. X450) •••••••• 58 7 High power view showing the immature collagen fibers of the periodontal ligament of a control animal (Gomori's reticulum stain. X450) •••••••••••- ••••••••• 59 vi LIST OF FIGURES Figure Page 8 High power view showing the immature collagen fibers of the periodontal ligament of an un­ treated adrenalectomized animal (Gornori's reticulum stain. X450) ••••••••••••••• 59 9 High power view showing the immature collagen fibers of the periodontal ligament of a cortisone treated adrenalectomized animal. Note the disorganization and decreased density of the fibers as compared to Figure 7 (Gomori's reticulum stain. X450) •••••••• 60 10 High power view of the periodontal ligament of a control animal showing the appearance of the ground substance (Alcian blue periodic acid-Schiff. X450) • • • • • • • • • • • • 60 11 High power view of the periodontal ligament of a untreated adrenalectomized animal. No appreciable difference can be noted in the ground substance as compared to the control animal shown in Figure 10 (Alcian blue periodic acid-Schiff. X450) •••••••••• 61 12 High power view of the periodontal ligament of a cortisone treated adrenalectomized rat. No appreciable difference can be noted compared to Figures 10 and 11 (A1cian blue periodic acid-Schiff. X450) •••••••••••• 62 vii I. INTRODUCTION Through the years, numerous investigations have been carried out concerning the eruption of the rat incisor. The basic factors responsible for tooth eruption are still unknown and only postulated theories are available. One prominent theory states that cell proliferation is the basic cause of incisor eruption. Sicher (1942) and Scott (1953) proposed that proliferation of pulpal cells within the tooth was the primary motivating force. On the other hand, other investigators hold the position that growth of the periodontal tissues is respon­ sible for eruption. Berkovitz (1972) concluded growth of the periodontal ligament to be the prime mover. Ness (1970) believed the fibroblasts of the periodontal ligament could be the critical impetus in the eruptive force. The present investigation will utilize the upper rat incisor in an effort to contribute additio~ al information toward a clearer solution to this question. The purpose of this study is to determine the effect of cortisone on fibroblast proliferation and connective tissue activity in the periodontal ligament of the rat incisor. With this study we hope to gain information about the influence of cortisone on this tissue as a possible factor in the eruptive process. 1 2 For a number of years, cortisone has been known to stimu­ late the rate of eruption in the rat incisor (Parmer, Katonah, and Angrist, 1951; Domm and Marzano, 1954; Goldsmith and Ross, 1956; Domm and Wellband, 1960). However, the effect of cortisone on the fibroblasts and connective tissue of the periodontal ligament at a dose level that adequately stimulates eruption is not clearly understood, Since cortisone is known to accelerate incisor eruption and in view of the fact that a number of investigators feel that growth of the periodontal ligament is the cause of this eruption, ....·..• .,. a study to determine the ~ffect of cortisone on the proliferative activity and connective tissue elements of the periodontal liga­ ment as a possible factor in the eruptive process would seem to be significant. II. REVIEW OF THE LITERATURE A. Experiments Concerned with the Mechanism of Tooth Eruption The mechanism
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