Saturday December 8, 2018
The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 25 7:30 am-7:00 pm Registration Open Registration Area 8:30 am-12:30 pm Special Interest Subgroups – Morning
A. 5th Biannual Frontiers in Cytokinesis Room 33B B. Building the Cell 2018 Room 30D C. Cell Biology in Cancer Immunity Room 31B D. Evolutionary Cell Biology Room 28D E. Intracellular Cargo Transport by Molecular Motors: What a Mesh! Room 29B F. Mechanisms of DNA Repair in Maintenance of Genome Integrity Room 29C G. Spatial and Temporal Analytical Tools for Cell Atlases Room 28B H. Systems and Synthetic Biology of Decoding Complex Cellular Rhythms Room 33C I. The Many Functions of Cytoskeletal Proteins in the Cell Nucleus Room 31C J. Wnt Signaling in Development and Cancer Room 30B
10:30-11:30 am Getting into Graduate School: The Do’s, the Don’ts, and the What If’s Room 15A 10:30-11:30 am Planning Your Exit from Graduate School Room 15B 11:45 am-12:45 pm Getting the Most out of Your Thesis Committee Room 15A 1:30-5:30 pm Special Interest Subgroups – Afternoon
K. Bottom-Up Cell Biology Room 30D L. Cellular Organization of Metabolism: Biology, Structure, Room 33C and Function of Enzyme Polymers M. Cilia and Cell Signaling in Development and Tissue Regeneration Room 29B N. Emerging Model Systems Room 29C O. Machine Learning in Cell Biology Room 31C P. Neuronal Cytoskeleton: A Complex Interplay of Cytoarchitecture Room 30B and Dynamics Q. Next Generation Correlative Microscopy: Biological Applications Room 31B and Emerging Techniques R. The Mechanics and Membrane Dynamics of the Nuclear Envelope Room 33B S. Patterning the Cytoskeleton - PTMs, MAPs, ABPs Room 28D T. The Midbody: From Cytokinesis to Signaling Organelle Room 28B
2:00-3:00 pm Hit the Ground Running: Early Success in Graduate School Room 15A 3:15-5:45 pm Judged Poster Session Room 16A Daily Schedule—Saturday, December 8 6:00 pm Keynote Lecture: Sean Morrison Ballroom 20B Immediately Following Keynote- Opening Night Reception Sails Pavilion 10:00 pm 8:00-9:00 pm International Research and Training Exchange Fair Sails Pavilion
26 The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org Saturday, December 8 SATURDAY OO Special Interest Subgroups – Morning
8:30 am-12:30 pm
The following member-organized sessions were selected by the ASCB Program Committee . All meeting attendees are welcome to SATURDAY participate . Meeting registration is required .
Subgroup A: 5th Biannual Frontiers in Cytokinesis Room 33B Organizers: Julie C. Canman, Columbia University; Ulrike Eggert, King’s College London; Amy Shaub Maddox, University of North Carolina, Chapel Hill; Douglas Robinson, Johns Hopkins School of Medicine; Dimitrios Vavylonis, Lehigh University; and Jian-Qiu Wu, Ohio State University
Cytokinesis is a spectacular cell shape change process that requires coordination of complex cellular machinery over many scales of space and time . Chemical and mechanical signaling pathways integrate the mitotic spindle with the cell cortex to position the division plane and promote assembly of a contractile actomyosin network, leading to remodeling of the plasma membrane and cortex . In a multicellular setting, cytokinesis also integrates cell-cell and cell-substrate communication . This geometrically simplified cell shape change serves as a paradigm for numerous other shape change events, including those that take place during cell migration and tissue morphogenesis . In this meeting, we bring together a group of investigators who use systematic genetic and chemical methods, biophysical techniques, high resolution imaging, diverse model systems, and mathematical modeling .
Presentations: 8:30 am Introduction . Organizers 8:35 am Control of cytokinesis by Plk1 in C . elegans . Sebastian Gomez-Cavazos, Oegema Lab, Ludwig Institute for Cancer Research 8:50 am Spindle to cortex communication in frog egg . Christine Field, Harvard University 9:10 am Actions ta microtubule plus ends in the phragmoplast for cytokinesis . Bo Liu, University of California, Davis 9:30 am Assembly and structure of the fission yeast cytokinetic ring . Kathy Gould, Vanderbilt University 9:50 am A theory to predict the contraction rate of random cytoskeleton network . François Nedelec, EMBL 10:10 am Work and dissipation in the cell cytoskeleton . Michael Murrell, Yale University 10:30 am Break 10:45 am The role of membrane shape in modulating cytokinetic furrow ingression . Jian Liu, National Institute of Heart, Lung, and Blood 11:05 am Flagella set the stage for myosin-independent cytokinesis in Giardia . Alex Paredez, University of Washington, Seattle 11:25 am Cleavage furrow formation without F-actin in Chlamydomonas . Masayuki Onishi, Pringle Lab, Stanford University 11:40 am Actin xidoreductiono as a novel component of the NoCut/abscission checkpoint . Jian Bai, Echard Lab, Institut Pasteur 11:55 am The role of calcium in Rho-dependent remodeling of epithelial tight junctions . Sara Varadarajan, Miller Lab, University of Michigan 12:10 pm Asymmetric division, stem cell size heterogeneities and cell fate . Agathe Chaigne, Paluch Lab, MRC Laboratory for Molecular Cell Biology . University College London, UK 12:25 pm Final remarks . Organizers
The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 27 Subgroup B: Building the Cell 2018 Room 30D Organizer: Susanne Rafelski, Allen Institute for Cell Science, Seattle WA
Modern cell biology has made great strides in understanding cell structure and function . As with any engineering problem, however, there is a third important aspect that needs to be understood besides structure and function, and that is assembly . How are the complex three-dimensional structures found within the cell specified by a one-dimensional genome? In this session we will explore the mechanisms by which cellular structures are determined and regulated . Because this question lies at the interface of biology and physics, this Building the Cell session will be highly interdisciplinary with speakers whose interests range from physics and mathematical modeling to biochemistry and cell biology .
Presentations: 8:30 am Introduction . Susanne Rafelski, Allen Institute for Cell Science 8:40 am Creating a stem cell state landscape: integrated cellular reorganization during differentiation and division of the human iPS cell . Susanne Rafelski, Allen Institute for Cell Science 9:00 am Building artificial membranes . Neal Devaraj, UC San Diego 9:20 am Understanding the heterotypic mitochondrial outer membrane fusion machine . Suzanne Hoppins, University of Washington 9:40 am The morphology space of yeast mitochondrial networks . Greyson Lewis, Wallace Marshall Lab, UC San Francisco 10:00 am Harnessing motors, flows, and fluctuations for intracellular transport . Elena Koslover, UC San Diego 10:20 am Break 10:40 am A fundamental trade-off between information flow in single cells and cellular populations . Eric J. Deeds, University of Kansas 11:00 am Dynamic architecture of the microtubule cytoskeleton . Marija Zanic, Vanderbilt University 11:20 am Super-resolution imaging of chromatin organization . Melike Lakadamyali, University of Pennsylvania 11:40 am Spreading of epigenetic silencing and activation in single cells . Lacramioara Bintu, Stanford University 12:00 pm Design principles for self-organized cell polarity . Ed Munro, University of Chicago
Subgroup C: Cell Biology in Cancer Immunity Room 31B Organizers: Xiaolei Su, Yale University; and Enfu Hui, University of California, San Diego
Cancer immunotherapy aims to activate or release the inhibition of immune cells for the sake of killing tumors . A variety of signaling molecules or immune effectors, including cell surface receptors, antibodies, and cytokines, have been targeted or engineered for eliminating cancer cells . Despite the clinical success in various immunotherapies, including checkpoint blockade and CAR-T, the cellular mechanism underlying how immune cells respond in those therapies remains unclear . This session will focus on recent progress in understanding and manipulating signaling events, transcriptional program, and reorganization of cellular structures that mediate immune cells’ responses to cancer antigens . It will not only advance our knowledge in understanding the molecular mechanism underlying cancer immunity but also provide insights in designing new strategies for cancer therapy .
Presentations: 8:30 am Introduction . Xiaolei Su, Yale University, and Enfu Hui, University of California, San Diego 8:35 am Phase Separation in Chimeric Antigen Receptor (CAR) Signaling . Xiaolei Su, Yale University 8:55 am Engineering Next-Generation T Cells for Cancer Immunotherapy . Yvonne Chen, University of California, Los Angeles 9:15 am Engineering the Next-Generation of Immune Cell Therapies for Cancer . Kole Roybal, University of California, San Francisco 9:35 am TCR Catch Bonds with Antigenic pMHC Activate T Cell Signaling and Its Implication for Effective Immunotherapy . Wei Chen, Zhejiang University 9:55 am Mechanopotentiation at the Cytotoxic Immunological Synapse . Morgan Huse, Memorial Sloan- Kettering Cancer Center 10:15 am Break 10:30 am Self-Cancellation of the PD-L1/PD-1 Pathway . Enfu Hui, University of California, San Diego
28 The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 10:50 am Understanding T Cell Inhibition and Costimulation to Improve Cancer Immunotherapy . Alice Kamphorst, Icahn School of Medicine at Mount Sinai 11:10 am Integration of Signals for Activation and Inhibition of NK Cells . Eric Long, National Institute of SATURDAY Allergy and Infectious Diseases 11:30 am Biomimetic Nanoparticles for Anticancer Vaccination . Liangfang Zhang, University of California, San Diego
11:50 am Engineering Phagocytic Receptors to Dissect Engulfment Signaling and Target Cancer . Adam SATURDAY Williamson, University of California, San Francisco 12:10 pm Using Microscopy to Inform the Engineering of Therapeutic Antibodies . Sally Ward, University of Southampton/Texas A&M University Health Science Center
Subgroup D: Evolutionary Cell Biology Room 28D Organizers: Holly V. Goodson, University of Notre Dame; and Margaret A. Titus, University of Minnesota
Evolutionary cell biology (ECB) encompasses research with two complementary goals: 1) using the perspectives and methods of evolutionary biology to gain insight into cell biological processes; and 2) studying the biology and diversity of cells to gain insight into the process of evolution . These goals are unified by the expectation that studying these questions in the context of cells the basic units of life—should illuminate fundamental principles of living systems . Talks in this session were chosen from abstracts submitted in response to a request sent to the ECB community . To build this community, we are compiling a list of ECB-related talks and posters to distribute at this session. To include yours in this list, please send your presentation information to the session organizers.
Presentations: 8:30 am Introduction . Holly V. Goodson, University of Notre Dame; Margaret A. Titus, University of Minnesota 8:35 am Conserved and divergent aspects of microtubule structure and dynamic instability . Sami Chaaban, McGill University, Montreal, Canada (Gary Brouhard Lab) 8:55 am How does a cell with no interphase microtubules build a mitotic spindle? Lillian Fritz-Laylin, University of Massachusetts Amherst, Amherst, MA 9:15 am Emerging mechanisms generating cytoskeletal complexity in the aggregatively multicellular Rhizarian amoeba Corallomyxa tenera . Sarah Guest, University of California Davis, Davis, CA (Scott Dawson Lab) 9:25 am Viral capsids traffic along tubulin filaments in Pseudomonas to reach the phage nucleus . Joseph Pogliano, University of California San Diego, San Diego, CA 9:45 am Break 10:05 am Organisation and evolution of the eukaryotic cell . Mark Field, University of Dundee, Dundee, Scotland 10:25 am Evolutionary origins of the nuclear pore complex and nucleus . Michael Rout, Rockefeller University, New York, NY 10:45 am The regulation of nuclear remodelling at mitotic exit . Gautam Dey, University College London, London, England (Buzz Baum Lab) 10:55 am Unraveling the origins of animal cell type differentiation . Sebastián R. Najle, Institute of Evolutionary Biology, Barcelona, Spain (Iñaki Ruiz-Trillo Lab) 11:05 am Break 11:25 am Evolutionary adaptation to replication stress reveals plasticity in DNA metabolism . Marco Fumasoni, Harvard University, Boston MA (Andrew Murray Lab) 11:35 am A tale of two actins: significant functional overlap of divergent actin isoforms in the unicellular green alga Chlamydomonas reinhardtii . Prachee Avasthi, University of Kansas Medical Center, Kansas City, KS 11:55 am The Hippo pathway, which regulates tissue size, is present in the closest unicellular relatives of animals . Jonathan Phillips, University of Texas Southwestern Medical Center, Dallas, TX (DJ Pan Lab) 12:05 pm Evolution and engineering of allosteric regulation in protein kinases . Kimberly Reynolds, University of Texas Southwestern Medical Center, Dallas, TX 12:25 pm Closing remarks . Holly Goodson and Margaret Titus
The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 29 Subgroup E: Intracellular Cargo Transport by Molecular Motors: What a Mesh! Room 29B Organizers: Gulcin Pekkurnaz, University of California San Diego; Sandra Encalada, The Scripps Research Institute; and David M. Warshaw, University of Vermont
Molecular motors are nanomachines that transport intracellular cargoes along a complex highway of cytoskeletal tracks that are composed of microtubules and actin filaments . How motor proteins deliver their cargo to its destination while overcoming physical challenges imposed by these complex filament meshworks is far from certain . Specifically, at every filament track intersection, motors are directionally challenged to navigate their cargo through this obstacle or barrier to transport . This special interest subgroup will bring together biophysicists and cell biologists interested in the molecular mechanisms that govern motor cargo transport using in vitro, in silico, and in vivo systems . Questions to be addressed include regulation of navigational motor decisionmaking, motor team work at filament intersections, the physical constraints imposed on motors and their cargo in crowded cellular environments, and how the interplay between motor, track, and cargo determine transport outcomes in both health and disease .
Presentations: 8:30 am Welcome. Sandra Encalada, The Scripps Research Institute 8:35 am Introduction . Gulcin Pekkurnaz, University of California San Diego 8:45 am Shedding new light on intracellular transport regulation with super-resolution microscopy . Melike Lakadamyali, University of Pennsylvania 9:15 am Microtubule network topology and its implications for cargo routing . Michael Vershinin, University of Utah 9:45 am Mechanisms of bi-directional microtubule-based motility . Sam Reck-Peterson, University of California, San Diego 10:15 am Break 10:30 am A cytoskeletal handoff regulates mitochondrial motility in dividing cells . Erika Holzbaur, University of Pennsylvania 11:00 am MAP7 recruits kinesin-1 to microtubules to target bidirectional cargoes to the plus end . Adam Hendricks, McGill University 11:30 am Cargo crowding leads to local traffic jams . Sandhya Koushika, Tata Institute of Fundamental Research 12:00 pm In vitro model of myosin cargo transport in a 3D actin network . David M. Warshaw, University of Vermont (and Closing remarks)
Subgroup F: Mechanisms of DNA Repair in Maintenance of Genome Integrity Room 29C Organizers: Ryan Jensen, Yale University School of Medicine; and Eli Rothenberg, New York University School of Medicine
The efficient maintenance of genome integrity and stability is vital for normal cellular functions and for prevention of diseases such as cancer . This session features research presentations from leaders in the field focusing on the intersection between DNA repair and DNA replication, genome stability, chromatin remodeling, cellular signaling, control of cell cycle checkpoints and cell death, and their implications for cancer etiology and treatment . Speakers will represent a diverse spectrum of approaches including: live cell imaging, biochemically reconstituted systems using purified proteins, super resolution microscopy to study nuclear dynamics of DNA repair complexes, replication stress and repair of DNA DSBs at stalled or collapsed replication forks, chromatin structure, and new technologies for studying DNA repair at the cellular and single molecule level .
Presentations: 8:30 am Opening and Intro . Ryan Jensen, Yale University, and Eli Rothenberg, NYU 8:35 am Fork Dynamics Determine Therapy Response in Hereditary Breast Cancer . Sharon Cantor, University of Massachusetts Medical School 8:55 am Maintaining the Integrity of the Genome within Chromatin . Kyle Miller, University of Texas at Austin 9:15 am Break Induced Replication: An unusual type of DNA synthesis promoting genomic instability . Anna Malkova, University of Iowa 9:35 am Illuminating the molecular functions of BRCA2 . Ryan Jensen, Yale University School of Medicine 9:55 am Repair of DNA Nicks: Implications for Cancer, Applications to Gene Therapy . Nancy Maizels, University of Washington 10:15 am Break
30 The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 10:25 am Understanding how error-prone DNA polymerases regulate the replication stress response . Tony Huang, NYU School of Medicine 10:45 am DNA Damage Response at the Crossroads of Repair and Apoptosis . Faye Rogers, Yale University SATURDAY School of Medicine 11:05 am Etiology of Chromosomal Rearrangements . Jeremy Stark, City of Hope 11:25 am Active or not – activity dependent mobility during DNA damage responses . Shan Zha, Columbia
University SATURDAY 11:45 am Targeting the ATR Checkpoint in Cancer Therapy . Lee Zhou, Harvard Medical School 12:05 pm Visualizing the cellular dynamics and organization of DNA repair proteins . Eli Rothenberg, NYU School of Medicine 12:25 pm Concluding remarks .
Subgroup G: Spatial and Temporal Analytical Tools for Cell Atlases Room 28B Organizers: Richard Conroy, National Institutes of Health; Jonah Cool, Chan Zuckerberg Initiative; and Sean Hanlon, National Institutes of Health
The rapid emergence of technologies for multiplexed and high throughput molecular mapping and phenotypic analysis of cells is driving development of multiscale, multidimensional cell atlases . New tools and common coordinate systems will be required to analyze and interpret these large datasets, and in particular to understand the role of spatial organization and temporal trajectories of the cells in a complex 3D tissue environment . In this session we will discuss emerging approaches and tools that provide robust, scalable, interoperable analysis of molecular and phenotypic information and are supporting community efforts to model cell-cell interactions, cell lineage and perturbations . There will be discussions with all speakers addressing challenges related to building interoperability between the datasets generated by different cell atlas initiatives .
Presentations: 8:30 am Introduction . Richard Conroy, National Institutes of Health, Jonah Cool, Chan Zuckerberg Initiative,Sean Hanlon, National Institutes of Health 8:40 am Kun Zhang, University of California, San Diego 9:10 am Emma Lundberg, KTH Royal Institute of Technology 9:40 am Long Cai, Caltech 10 10. am Break 10:30 am Junhyong Kim, University of Pennsylvania 11:00 am Joe Gray, Oregon Health & Science University 11:30 am Leeat Keren, Stanford University 12:00 pm Dana Pe’er, Memorial Sloan Kettering Cancer Center
Subgroup H: Systems and Synthetic Biology of Decoding Complex Cellular Rhythms Room 33C Organizer: Qiong Yang, University of Michigan, Ann Arbor
Cell-autonomous oscillators, from sub-second action potentials to 24-hour circadian clocks, are fundamental and prevalent throughout living systems, exhibiting complicated oscillations, waves, and patterns . Despite their diversity and complexity, technology development in systems and synthetic biology triggers enormous interest and enables characterization of this universal phenomenon . In this session, a group of theorists and experimentalists will meet and discuss important topics on cellular rhythms from various perspectives crossing disciplines, e .g ., principles shared among oscillators, synthetic clocks of minimal design and desired functions, tools for reconstituting more complicated dynamics and sub-cellular organization, and emerging spatiotemporal patterns from coupled oscillators . This subgroup, by bringing together biologists, physicists, and engineers with shared interests who rarely meet each other, will provide an opportunity to forge a new network of potential collaborators .
Presentations: 8:30 am Introduction . Qiong Yang, University of Michigan, Ann Arbor 8:35 am From molecules to development: how clocks function and coordinate . Qiong Yang, University of Michigan, Ann Arbor 8:50 am Cell cycles on cell trees . Stanislav Shvartsman, Princeton University 9:10 am Trigger waves in cell signaling . James Ferrell, Stanford University
The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 31 9:30 am Lilianna Solnica-Krezel, Washington University in St . Louis 9:50 am Cell cycle in early embryo development and the onset of zygotic genome activation . Matt Good, University of Pennsylvania 10:10 am Engineering DNA programmed dynamical systems using a cell-free expression toolbox . Vincent Noireaux, University of Minnesota 10:30 am Genetic oscillations in a spatially extended synthetic microbial consortium . Matthew Bennett, Rice University 10:50 am Oscillatory hormonal control of mammalian cell differentiation . Mary Teruel, Stanford University 11:10 am Functional olesr of inorganic ions in the cell . Gürol M. Süel, University of California, San Diego 11:30 am Scaling of oscillation-based pattern . Sean Megason, Harvard Medical School 11:50 am From single-cell oscillations to aggregates: Identifying the key control parameters driving collective signaling in Dictyostelium . Allyson Sgro, Boston University 12:10 pm Signaling oscillations during embryonic patterning . Alexander Aulehla
Subgroup I: The Many Functions of Cytoskeletal Proteins in the Cell Nucleus Room 31C Organizers: Piergiorgio Percipalle, New York University Abu Dhabi; and Maria Vartiainen, Institute of Biotechnology, University of Helsinki
The emerging role of cytoskeletal proteins in the cell nucleus has become a new frontier in cell biology . Actin and actin-binding proteins regulate chromatin and gene expression, but importantly they are beginning to be essential players in genome organization in both prokaryotic and eukaryotic cells . These global actin-based functions contribute to genome stability and integrity while affecting DNA replication and global transcription patterns . This is likely to occur through interactions of actin with nuclear components including nuclear lamina and subnuclear organelles . An exciting future challenge is to understand how these actin-based genome- wide mechanisms may regulate development and differentiation by interfering with the mechanical properties of the cell nucleus and how regulated actin polymerization plays a role in maintaining nuclear architecture . This session will bring together scientists from different angles and disciplines to discuss and elucidate—possibly through unpublished, ongoing work—how cytoskeletal proteins act to consolidate nuclear architecture for sustained gene expression or silencing during the acquisition of cellular identity .
Presentations: 8:30 am Introduction . Piergiorgio Percipalle, New York University Abu Dhabi, and Maria Vartiainen, Institute of Biotechnology, University of Helsinki 8:35 am Functional interactions between actin, ARPs and chromatin . Masahiko Harata, Tohoku University, Sendai, Japan 8:55 am Function of nuclear actin inenome g stability . Kenji Shimada, Friedrich Miescher Institute for Biomedical Research, Basel 9:15 am Nuclear actin in chromatin organization and nuclear reprogramming . Piergiorgio Percipalle, New York University Abu Dhabi 9:35 am Actin polymerization in reprogramming nuclear structures . Kei Miyamoto, Kindai University 9:55 am Actin’ on transcription . Maria Vartiainen, University of Helsinki 10:15 am Nuclear WASp regulates transcription networks in developing T cells by interaction with TCF1 . Lisa Westerberg, Karolinska Institute Break 10:55 am Generation of DNA repair domains . Jean Gauthier, Columbia University 11:15 am Highways for repair: nuclear F-actin and myosins drive relocalization of heterochromatic DNA damages . Irene Chiolo, University of Southern California, Los Angeles 11:45 am Exploring the world of nuclear actin and actin PTMs . Xuetong Shen, MD Anderson Cancer Center 12:05 pm Viral mobilization of nuclear actin . Matthew Welch, University of California, Berkeley 12:25 pm Concluding Remarks
32 The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org Subgroup J: Wnt Signaling in Development and Cancer Room 30B
Organizers: Henry Ho, University of California, Davis; and Taran Gujral, Fred Hutchinson Cancer Research Center SATURDAY
This session focuses on Wnt signaling pathway and its role in embryonic development and cancer . Wnt family proteins are growth factors that play critical roles in proliferation, migration, and invasion . Aberrant activation of Wnt signaling has been implicated in
a variety of human developmental disorders and in malignancies of the colon, breast, liver, skin, brain, and prostate . We will hear SATURDAY from leading experts who study the role of Wnt in normal development and in disease states .
Presentations: 8:30 am Introduction . 8:35 am Intestinal Wnt signaling . Calvin Kuo, Stanford University, University 9:00 am WNT-FZD specificity in stem cells . Karl Willert, University of California, San Diego 9:25 am New insights into Wnt signaling, vertebrate development, and stem cells . Xi He, Harvard Medical School 9:50 am Wnt Signaling Connections to Stem Cells and Invasion in Colon Cancer . Marian Waterman, University of California, Irvine 10:15 am Break 10:35 am Ubiquitin-mediated regulation of the Wnt Pathway . Ethan Lee, Vanderbilt University 11:00 am Wnt-er tales: Post genomic regulation of Wnt responsive cells by modulation of Cul4/DDB1/ DCAF4 ubiquitin ligase activity . Timothy F. Lane, University of California, Los Angeles 11:25 am Non-canonical Wnt signaling in Cancer . Taran Gujral, Fred Hutchinson Cancer Research Center 11:50 am Mechanisms of Wnt5a-Ror signaling in development and disease . Henry Ho, University of California, Davis 12:15 pm Closing . General Questions and Answers
OO Getting into Graduate School: The Do’s, the Don’ts, and the What If’s
10:30-11:30 am Room 15A Tama Hasson, Assistant Vice Provost for Undergraduate Research, University of California, Los Angeles Leticia Vega, Associate Professor, Barry University
This session is designed specifically to inform undergraduate attendees about the ins and outs of applying to and getting into graduate school (MS or PhD) or MD/PhD programs . Topics addressed will include timelines for admission, requesting letters of recommendation, crafting personal statements, the interview process, and potential pitfalls in the application process . The speakers have years of experience mentoring undergraduates through the application process and will answer students’ questions to help them become stronger applicants .
Outcomes: 1 . Acquire broad-based knowledge regarding the process of applying to graduate school or MD/PhD programs, including the application timeline . 2 . Gain an understanding of the importance of a diversity statement in your personal statements . 3 . Appreciate the importance of the ASCB in your professional development .
Target audience: undergraduates, postbaccalaureates, MS students, and their mentors
The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 33 OO Planning Your Exit from Graduate School
10:30-11:30 am Room 15B Moderators: Ahna Skop, Faculty, University of Wisconsin-Madison Jim Vigoreaux, Associate Provost & Faculty; University of Vermont Giovanna Guerrero-Medina, Director, Yale University & Ciencia Puerto Rico
Postdocs: Elisabeth Marnik, MDI Biological Laboratory Gaia Gantelli, Duke University Krishnakumar Vasudevan, Stanford University
Transitioning out of graduate training, whether it’s into postdoctoral training or the workforce, can be a daunting prospect for graduate students . It requires forethought and planning—with time to network, interview, and settle on next steps—and organizational skills to accomplish all of this while conducting research or writing a thesis . In this session, panelists will discuss: timelines for planning; the importance of networking, as well as strategies, tips and platforms for networking; the role of research advisors and other mentors; tools, such as the IDP, to help prioritize options, identify needs, and plan to work toward career objectives; preparing for interviews for postdoctoral and professional positions; juggling research and graduation responsibilities with exiting priorities; and managing challenges related to mentors or graduate program requirements .
Students will receive a worksheet to help them plan and organize their exit from graduate school . Panel members will demonstrate a diversity of gender, social identity, career outcomes (e .g ., postdoc/academic, industry, non-research) and stages (junior to senior) .
Outcomes: 1 . Gain an understanding of the ideal timelines and steps required to successfully exit graduate school and transition into the next academic or professional step . 2 . Acquire concrete strategies and resources to explore your career objectives and to work toward them . 3 . Demonstrate increased confidence in your ability to manage tasks related to exiting graduate school .
Target audience: late-stage graduate students
OO Getting the Most out of Your Thesis Committee
11:45 am-12:45 pm Room 15A Sarah Cohen, Assistant Professor, Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill Ernest Heimsath, Postdoctoral Fellow, Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill Brian Lewis, Associate Professor , Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School James Olzmann, Assistant Professor, Department of Nutritional Sciences and Toxicology, University of California, Berkeley
A graduate student’s thesis committee, a requirement for most programs, is an incredibly important factor in a graduate student’s training and career advancement . Ideally, the committee will be a resource for scientific and career advice, mentorship, sponsorship, oversight, and potentially advocacy . While the importance of the thesis committee is evident, advice on how to select, interact with, and leverage committee members is often implicit . A dynamic presentation, utilizing polls and pair-shares to encourage active learning, will provide a brief overview of: the thesis committee’s role, considerations in selecting committee members, strategies for effective thesis committee meetings, and strategies for productive relationships with committee members . Following the presentation, the panel will further discuss these topics and provide lived-insights, advice, and context .
34 The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org Outcomes: 1 . Gain a better understanding of the potential impact a thesis committee can have on thesis research progress . SATURDAY 2 . Acquire strategies for selecting a thesis committee, establishing mentoring relationships with its members, and preparing for and holding a thesis committee meeting . 3 . Gain increased confidence in the ability to utilize the thesis committee for scientific and academic success . SATURDAY Target audience: graduate students
OO Special Interest Subgroups – Afternoon
1:30-5:30 pm The following member-organized sessions were selected by the ASCB Program Committee . All meeting attendees are welcome to participate . Meeting registration is required .
Subgroup K: Bottom-Up Cell Biology Room 30D Organizers: Daniel A. Fletcher, University of California, Berkeley; and Matthew C. Good, University of Pennsylvania
In vitro reconstitution of biological processes from their component molecular parts is a mainstay of biochemistry and has emerged over the last decade as a powerful tool in cell biology . Recent studies have shown that cell-like structures with micron-scale organization can be reconstituted from nanometer-scale parts by combining purified proteins and cytoplasmic extracts with cell-like boundary conditions . By identifying the necessary and sufficient conditions for assembly, these ‘bottom-up’ studies provide new mechanistic insight that complements more traditional ‘top-down’ cell biology . Rapid progress in micropatterning, microfluidics, and microfabrication, coupled with continued advancements in biochemistry and molecular biology, raise the possibility of creating more complete cellular reconstitutions that may one day rival the complexity of live cells .
Presentations: 1:30 pm Introduction: Matt Good,University of Pennsylvania, and Dan Fletcher, University of California, Berkeley 1:35 pm Dissecting the proofreading mechanisms of endocytosis through reconstitution . Min Wu, National University of Singapore 1:50 pm Reconstitution of an active actin-membrane composite . Satyajit Mayor, NCBS, Bangalore 2:10 pm Reconstitution of the dynamic steady state of actin networks . Laurent Blanchoin, BBI, Grenoble 2:30 pm Reconstitution and biophysical study of functional kinetochores . Chip Asbury, University of Washington 2:50 pm How cells sense micron-scale curvature using the septin cytoskeleton. Amy Gladfelter, University of North Carolina at Chapel Hill 3:10 pm Self-organization of the bacterial cell division machinery . Martin Loose,IST, Austria 3:30 pm Reconstituting the Molecular Mechanisms of Membrane Traffic . Jeanne Stachowiak, University of Texas 3:50 pm Autonomously Self-regulating Giant Vesicles . Atul Parikh, University of California, Davis 4:10 pm Buckling of epithelium growing under spherical confinement . Aurelien Roux, University of Geneva 4:30 pm Reconstructing phase transitions and kinetic proof reading in the T cell receptor signaling system . Jay Groves, University of California, Berkeley 4:50 pm Reconstitution of dendritic cell function . Michael Dustin,New York University 5:10 pm Synthetic Notch circuits to direct multicellular organization . Wendell Lim, University of California, San Francisco
The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 35 Subgroup L: Cellular Organization of Metabolism: Biology, Structure, and Function of Enzyme Polymers Room 33C Organizers: Justin Kollman, University of Washington; and Jeffrey Peterson, Fox Chase Cancer Center
An increasing number of enzymes dynamically and reversibly assemble into cellular structures in response to changes in nutrient availability or other environmental cues . These structures represent a novel non-membrane-bound mechanism for compartmentalization and localization of enzymatic activity . Many of the enzymes that undergo dynamic reorganization are metabolic enzymes (e .g ., CTP synthase, inosine monophosphate dehydrogenase, and phosphofructokinase) . The discovery of complex and dynamic spatial organization of enzymes opens an exciting new field at the interface between cell biology and metabolism . Most examples of dynamically reorganizing metabolic structures remain functionally uncharacterized, but the few examples that are characterized suggest that this larger scale organization plays fundamental roles in enzyme activity and for maintaining cellular homeostasis . Important questions remain for many metabolic enzyme structures: What are their mechanisms of assembly and disassembly? What is their biological function? How is assembly regulated? The presentations in this subgroup will discuss recent advances in addressing these questions, both in vitro and in vivo, for a variety of polymerizing enzymes .
Presentations: 1:30 pm Emergent physical and cellular properties of synthetic, infinite protein assemblies . Emmanuel Levy, Weizmann Institute 1:50 pm Supramolecular Assembly of Metabolic Enzymes in Saccharomyces cerevisiae . Chalongrat Noree, Mahidol University 2:10 pm Controlling the activity of eukaryotic acetyl-CoA carboxylases: conformational locking and polymerization . Timm Maier, University of Basel 2:30 pm Filament formation by the Phosphofructokinase-1, the gatekeeper of glycolysis . Bradley Webb, West Virginia University 2:50 pm Metabolite regulation of stress granule composition and assembly . James Wilhelm, University of California, San Diego 3:10 pm Histidine-mediated protein methylation and CTPS compartmentalization . Li-Mei Pai, Chang Gung University 3:30 pm Break 3:50 pm The structural basis for regulation of nucleotide biosynthesis enzymes by polymerization . Justin Kollman, University of Washington 4:10 pm The assembly of the cytoophidium . Ji-Long Liu, Shanghai Tech University 4:30 pm IMPDH assembly during T cell activation . Jeffrey Peterson, Fox Chase Cancer Center 4:50 pm Immune response-dependent assembly of IMP dehydrogenase filaments (rods/rings structures) . Edward Chan, University of Florida 5:10 pm The role of rods and rings in neurodegeneration . Naiara Akizu, Children’s Hospital of Philadelphia
Subgroup M: Cilia and Cell Signaling in Development and Tissue Regeneration Room 29B Organizers: Peter Jackson, Stanford University; and Jeremy Reiter, University of California, San Francisco
Primary and motile cilia provide critical sensory, neuroendocrine, and metabolic control in specific tissues . Studies of the cell biology of cilia, from model systems and human ciliopathies, has led to considerable insight into ciliary function, demonstrating the importance of cilia both in early development and also in controlling degeneration of tissue in pediatric and adult settings . This regenerative role is exemplified by signaling in the Sonic Hedgehog pathway, but less well understood defects lead to liver/ kidney fibrosis, airway epithelia, skin, mesenchymal tissue, brain degeneration and cancer . Here we present speakers with new understanding of signaling mechanisms and structures, the elucidation of cilia signaling pathways, and animal models reflecting the importance of cilia in stem cells, tissue regeneration, and tissue pathogenesis .
Presentations: 1:30 pm Introduction, New Signaling Mechanisms in Cilia . Peter Jackson, Stanford University 1:50 pm Specification of cilia subtypes required for developmental signaling . Chris Kintner, Salk Institute 2:10 pm Feel the beat – multi-ciliated cell formation in the airway epithelium . Aron Jaffe, Novartis 2:30 pm The role of cilia in melanoma . Lukas Sommer, University of Zurich 2:50 pm Ciliary signaling in development and disease . Saikat Mukhopadhyay, University of Texas Southwestern
36 The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 3:10 pm Loss of primary cilia drives Hedgehog to Ras pathway switching in resistant basal cell carcinoma . Francois Kuonen, Stanford University 3:25 pm Omega-3 fatty acid activation of ciliary FFAR4 receptors on perivascular preadipocyte stimulates SATURDAY adipogenesis . Keren Hilgendorf, Stanford University 3:40 pm Break . 3:55 pm Ciliary subdomains and signaling . Jeremy Reiter, University of California, San Francisco
4:10 pm Structure and function of the BBSome . Maxence Nachury, University of California, San Francisco SATURDAY 4:30 pm Cilia Assembly and Maintenance in Chlamydomonas . Karl Lechtreck, University of Georgia 4:50 pm Cilia maintain the synaptic architecture of excitatory cortical neurons . Piali Sengupta, Brandeis University 5:10 pm Ciliary extracellular vesicles: from biogenesis to bioactivity . Maureen Barr, Rutgers University
Subgroup N: Emerging Model Systems Room 29C Organizers: Mansi Srivastava, Harvard University; and Bob Goldstein, University of North Carolina at Chapel Hill
Many fascinating questions in cell biology have been set aside for generations because they involve phenomena that aren’t found in well-established model systems . But this situation is improving, as techniques developed in popular model systems are increasingly applied to other organisms—leading to a recent flowering of emerging and re-emerging models suited to answering diverse questions . Moreover, the study of diverse non-model organisms has led to the discovery of new phenomena that may have widespread importance . In this session, speakers will present cutting-edge results from diverse emerging model systems . This session will feature all new speakers and emerging model systems than in the two earlier incarnations of this subgroup session . The session will end with a Q&A panel in which all speakers will answer questions about topics relevant to studying emerging model systems, for example challenges in getting started with a new model, approaches for sharing organisms and methods, strengths and limitations of emerging models, funding, and career development prospects .
Presentations: 1:30 pm Introduction . Bob Goldstein, University of North Carolina at Chapel Hill; and Mansi Srivastava, Harvard University 1:35 pm The cellular basis for structural color in butterflies . Nipam Patel, Marine Biological Laboratory, Woods Hole 1:52 pm Developing model systems to study organelle biology in bacteria . Arash Komeili, University of California at Berkeley 2:09 pm The Hawaiian bobtail squid: a model organism for studying host-microbe interactions . Spencer Nyholm, University of Connecticut 2:26 pm Rising tide: xploringe biology within the sea with brown algae . Siobhan Braybrook, University of California, Los Angeles 2:43 pm Plasticity of meiotic mechanisms: insights from the nematode Pristionchus pacificus . Abby Dernburg, University of California, Berkeley 3:00 pm Studying aging and diapause in vertebrates using the short-lived African killifish . Anne Brunet, Stanford University 3:17 pm Break 3:35 pm Spiny mice as an emerging model to investigate the cellular regulation of mammalian regeneration . Ashley Seifert, University of Kentucky 3:52 pm Embryonic origins and cell cycling behavior of annelid stem cells . B. Duygu Özpolat, Marine Biological Laboratory, Woods Hole 4:09 pm The sex lives of parasitic schistosomes: it’s complicated . James Collins, University of Texas Southwestern 4:26 pm Stem cell differentiation in Hydra at single cell resolution . Celina Juliano, University of California, Davis 4:43 pm Life in flatland: Linking tissue architecture, mechanics and morphogenesis in a simple metazoan model, Trichoplax adhaerens . Manu Prakash, Stanford University 5:00 pm Question and answer session
The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 37 Subgroup O: Machine Learning in Cell Biology Room 31C Organizer: Kwonmoo Lee, Worcester Polytechnic Institute
Quantitative cell biology underwent dramatic growth over the last decade due to a wide range of image analysis algorithms as well as advanced microscopy . This enables researchers to quantitatively measure cellular and subcellular phenomena in unpreceded detail, and build various datasets of cell biological processes, including cell motility, cytoskeleton, and membrane-bound organelles . Recently, machine learning has been making tremendous progress and has shown that computers can outperform humans in the analysis of complex high dimensional datasets . Thus, machine learning has great potential to extract hidden information from heterogeneous cell image datasets and provide detailed mechanistic biological insights . The session will showcase exciting applications of machine learning in various cell biological problems and present novel machine learning techniques that can be applied in cellular image analysis .
Presentations: 1:30 pm Introduction . Kwonmoo Lee, Worcester Polytechnic Institute 1:35 pm Machine learning of the assembly instructions of a cell . Robert Murphy, Carnegie Mellon University 2:00 pm Inferring cell state dynamics with machine learning methods . Jacob Kimmel, Calico Life Sciences and University of California, San Francisco 2:25 pm Deconvolution of subcellular protrusion heterogeneity by machine learning-based live cell analysis . Kwonmoo Lee, Worcester Polytechnic Institute 2:50 pm A machine learning framework for modeling the structure and function of a cell . Trey Ideker, University of California, San Diego 3:15 pm Break 3:25 pm Machine learning for cell organization: new methods to capture variation and integrate observations . Greg Johnson, Allen Institute for Cell Science 3:50 pm High-resolution characterization of complex organelle morphology using deep convolutional networks . Ge Yang, Carnegie Mellon University 4:15 pm Machine learning and computer vision approaches for phenotypic profiling in yeast . Brenda Andrews, University of Toronto 4:40 pm Live cell histology for classification of melanoma cell population based on single cell actions . Assaf Zaritsky, Ben-Gurion University of the Negev and University of Texas Southwestern Medical Center 5:05 pm Context-aware predictions for tracking and segmentation . Jan Funke, Howard Hughes Medical Institute Janelia Farm
Subgroup P: Neuronal Cytoskeleton: A Complex Interplay of Cytoarchitecture and Dynamics Room 30B Organizers: Kassandra Ori-McKenney, University of California, Davis; and Le Ma, Thomas Jefferson University
The architecture of the neuronal cytoskeleton powers the development and plasticity of a functional nervous system . This is accomplished through cytoskeletal-generated forces that power morphological changes in neurons, including neuronal migration, axon outgrowth, dendritic arborization and synaptogenesis . In addition, the neuronal cytoskeleton provides tracks for intracellular membrane and organelle transport and delivery, which can be regulated by cytoskeletal-associated proteins . Advanced molecular, genetic, and imaging techniques allow for molecular interrogation and analysis of cytoskeletal architecture and dynamics as well as intracellular trafficking in the neuron with high spatial and temporal resolution . This session will highlight novel findings and mechanistic insights into this exciting area of neuronal cell biology, and how these programs may go awry in neurodevelopmental and neurodegenerative disease .
Presentations: 1:30 pm Introduction . Kassandra Ori-McKenney, University of California, Davis; and Le Ma, Thomas Jefferson University 1:35 pm New insights into organization and dynamics of axonal actin . Subhojit Roy, University of Wisconsin . 1:55 pm Patterning of the axonal microtubule cytoskeleton . Shaul Yogev, Yale University . 2:15 pm Coordinating membrane and actin cytoskeleton remodeling at the synapse . Avital Rodal, Brandeis University
38 The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 2:35 pm Role of microtubule acetylation and aTAT1 in shaping neuronal development . Jill Wildonger, University of Wisconsin 2:55 pm Cytoskeletal reorganization in response to stroke and other acute neuronal injuries . Shelley SATURDAY Halpain, University of California, San Diego 3:15 pm Doing more work with less F-actin during chemotropic growth responses: new perspectives on the molecular clutch hypothesis . Paul Forscher, Yale University
3:35 pm Break SATURDAY 3:50 pm Doublecortin defines a zone of microtubule nucleation and regrowth in growth cones . Gary Brouhard, McGill University 4:10 pm MAP7 regulates microtubule stability and organelle transport during axon branch development . Stephen Tymanskyj, Thomas Jefferson University 4:30 pm Lattice gating by microtubule-associated proteins differentially directs neuronal motor transport . Kassandra Ori-McKenney, University of California, Davis 4:50 pm Multiple amiliesf of motors act independently to drive the axonal transport of vesicles to the synapse . Sandra Encalada, The Scripps Research Institute 5:10 pm Novel roles for cytoplasmic dynein adaptor proteins (RDDs) . Richard Vallee, Columbia University
Subgroup Q: Next Generation Correlative Microscopy: Biological Applications and Emerging Techniques Room 31B Organizers: Ori Avinoam, Weizmann Institute of Science, Rehovot, Israel; and Yannick Schwab, European Molecular Biology Laboratory, Heidelberg Germany
Gaining a molecular level understanding of biological processes depends on developing the capability to visualize the molecules involved relative to their location within the cell at high resolution . However, obtaining such high-resolution information on dynamic processes in situ often requires the application of more than one imaging technology to the exact same substrate by correlative microscopy . At the crossroad of multidisciplinary approaches, correlative microscopy brings together biologists, image analysts, and engineers, leading to powerful workflows that are applied to an ever-increasing number of biological questions and models . This session will highlight the most recent advances in correlative microscopy and their application to unravel cellular organization and the molecular function of protein machineries in their subcellular context .
Presentations: 1:30 pm Introduction . Ori Avinoam, Weizmann Institute of Science, Rehovot, Israel, and Yannick Schwab, European Molecular Biology Laboratory, Heidelberg Germany 1:40 pm Transcytosis balances apical-basal membrane sorting during subcellular lumen formation: what do we learn from CLEM . Maria Leptin, European Molecular Biology Laboratory, Heidelberg, Germany 2:10 pm Large volume correlative light electron microscopy to obtain functional insights on endomembrane organization and its subversion by pathogens . Jost Enninga, Institut Pasteur, Paris, France 2:30 pm Life, imaged Live: monitoring early metastatic events with intravital and correlative microscopy . Matthia Karreman, Germany Cancer Research Institute (DKFZ), Heidelberg, Germany 2:50 pm Combining cryo fluorescence microscopy with cryo electron tomography to study native cellular membranes . Wanda Kukulski, MRC Laboratory of Molecular Biology, Cambridge, UK 3:10 pm Break 3:30 pm New insights to cellular composition by scanning transmission electron cryo-microscopy . Michael Elbaum, Weizmann Institute of Science, Rehovot, Israel 3:50 pm Correlated cryofluorescent and soft x-ray tomography . Carolyn Larabell, University of California, San Francisco 4:10 pm A multimodal imaging approach to dissect the role of endocytosis during secretion . Kamalesh Kumari, Weizman Institute of Science, Rehovot, Israel 4:30 pm Comulus, a new network to bridge (pre) clinical imaging and light electron microscopy . Perrine Paul-Gilloteaux, CNRS France-BioImaging, Nantes, France 4:50 pm Large volume electron microscopy imaging and its correlation to cryo-fluorescence microscopy . Harald F. Hess, Janelia Research Campus 5:20 pm Question and answer panel with all speakers
The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 39 Subgroup R: The Mechanics and Membrane Dynamics of the Nuclear Envelope Room 33B Organizers: Christian Schlieker, Yale University; and Shirin Bahmanyar, Yale University
It has become clear that the textbook definition of the nuclear envelope as a stable structure is no longer accurate . Nuclear envelope dynamics, which includes membrane fusion, rupture and repair, and budding, allows for nuclear pore complex insertion, constricted cell migration, and viral proliferation . Defects in nuclear envelope dynamics are associated with heterogeneous diseases including muscular dystrophy, movement disorders, aging, and cancer . By bringing together researchers from diverse fields that intersect in nuclear envelope biology, we will explore the mechanics and dynamics of the nuclear envelope and how mis-regulation of these processes contributes to disease . The insight gained will provide a more comprehensive view of nuclear envelope function that extends well beyond its long-assumed role as a static barrier between the nucleoplasm and cytoplasm .
Presentations: 1:30 pm Opening Remarks . Christian Schlieker, Yale University, and Shirin Bahmanyar, Yale University 1:35 pm Crosstalk between chromatin and nuclear mechanics . Megan King, Yale School of Medicine 1:55 pm Nuclear envelope rupture, DNA damage, and DNA damage response activation as drivers of lamin-associated muscular dystrophy . Jan Lammerding, Cornell University 2:15 pm The great nuclear escape: mechanism of membrane budding during the nuclear egress of herpesviruses . Katya Heldwein, Tufts University 2:35 pm New insight into nuclear pore complex assembly . Guillaume Holzer (Antonin Lab), Aachen University 2:55 pm Torsin ATPases and nuclear envelope dynamics . Christian Schlieker, Yale University 3:15 pm The ER-resident AAA+ ATPase TorsinA and its unusual oligomerization . Thomas Schwartz, Massachusetts Institute of Technology 3:35 pm Break 3:50 pm The nuclear envelope surveillance machinery is segregated by and directly monitors the nuclear transport system . Patrick Lusk, Yale School of Medicine 4:10 pm Coordinating dynamic events of nuclear assembly . Katie Ullman, University of Utah 4:30 pm Coordinating ER membrane biogenesis with nuclear envelope dynamics . Shirin Bahmanyar, Yale University 4:50 pm Lipid metabolism of the inner nuclear membrane . Alwin Kohler, University of Vienna 5:10 pm Concluding remarks
Subgroup S: Patterning the Cytoskeleton - PTMs, MAPs, ABPs Room 28D Organizers: Antonina Roll-Mecak, National Institutes of Health; and Kristen Verhey, University of Michigan
The cytoskeleton consists of three interconnected filamentous networks—microtubules, actin filaments, intermediate filaments— that play critical roles in cell structure, division, migration, and intracellular trafficking . All microtubules are assembled from tubulin subunits and all actin filaments are assembled from actin monomers . Yet cells can generate specialized microtubule and actin filament structures with distinct spatial and temporal patterns and distinct functional outputs . How these specialized filaments are generated by cells and then “read” by filament-associated proteins is an area of active research . Recent work has shown that diversity in filament architecture can be provided by different actin or tubulin isotypes as well as chemically diverse posttranslational modifications (PTMs) . In addition, diverse filament architectures are specified and regulated by a large number of accessory factors such as microtubule associated proteins (MAPs) and actin binding proteins (ABPs) . In this subgroup, we will explore a variety of mechanisms for generating specific cytoskeletal structures (PTMs, MAPs, ABPs) as well as the functional output of these structures in a variety of experimental systems (yeast, flies, worms, mice, cell culture) .
Presentations: 1:30 pm Introduction . Antonina Roll-Mecak, National Institutes of Health 1:35 pm Self-organization of the actin cytoskeleton for diverse functions in fission yeast . Dave Kovar, University of Chicago 1:55 pm Site-specific oxidation by Mical primes actin for rapid disassembly . Elena Grintsevich, University of California, Los Angeles 2:15 pm Reversible Redox post-translational modification of actin to control cellular form and function . Jonathan Terman, University of Texas Southwestern 2:35 pm Microtubules Gate the Condensation of Tau to Locally Regulate Microtubule Function . Rick McKenney, University of California, Davis
40 The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 2:55 pm MAP6 proteins: from microtubule stabilization to actin dynamics in neurons . Annie Andrieux, CNRS Grenoble 3:15 pm Break SATURDAY 3:35 pm Microtubule organization during anaphase in human cells . Tarun Kapoor, Rockefeller University 3:55 pm How to find readers of the tubulin code? Carsten Janke, Institute Curie, Paris 4:15 pm Methylation versus acetylation at alpha tubulin K40 . Kristen Verhey, University of Michigan
4:35 pm Tubulin PTMs and their regulation of the heartbeat . Ben Prosser, University of Pennsylvania SATURDAY 4:55 pm Microtubule dynamics away from the tips . Antonina Roll-Mecak, National Institutes of Health 5:15 pm General discussion with all speakers
Subgroup T: The Midbody: From Cytokinesis to Signaling Organelle Room 28B Supported by Bruker Corporation and 3i Intelligent Imaging Innovations Organizers: Ahna R. Skop, University of Wisconsin-Madison; and Arnaud Echard, Institut Pasteur, Paris
The relatively new field of midbody biology is attracting intense interest beyond its role in cytokinesis, as midbodies have been recently implicated in the fundamental control of cell architecture, fate, polarity, tumorigenicity, and pluripotency . And yet some of the most basic questions about midbody function remain unclear and at times controversial: How are midbodies generated during cytokinetic abscission? Do midbodies have post-mitotic functions and, if so, what are they and how are they regulated? How much variation in midbody function exists among distinct cell types? What proportion of post-mitotic midbodies are inherited intracellularly versus extracellularly? Do midbodies mediate informational transfer via an intracellular mechanism, an extracellular mechanism, neither, or both? What roles do midbody protein play in promoting cancer cell fate and pluripotency? It is necessary that models of midbody function must become more detailed, mechanistically speaking, to distinguish among these options . In this session, we will highlight the current research on the midbody in hopes to uncover many of these questions .
Presentations: 1:30 pm Introduction: what is the midbody and why should you care? Ahna Skop, University of Wisconsin- Madison 1:45 pm From the contractile ring to the midbody ring: a maturation process . Gilles Hickson, Université de Montréal 2:10 pm The midbody remnant proteome or Flemmingsome reveals new proteins required for cytokinetic abscission and post-abscission events . Arnaud Echard, Institut Pasteur – CNRS, Paris, France 2:35 pm Characterization of the midbody interactome reveals roles for PP1 phosphatases in late cytokinesis . Paolo D’Avino, University of Cambridge, UK 3:00 pm The last chance saloon: The midbody-associated C. elegans LEM-3 nuclease mends multiple DNA intermediates just before cells divide . Anton Gartner, University of Dundee 3:25 pm The role of post-mitotic midbodies in regulating cell proliferation and differentiation . Rytis Prekeris, University of Colorado, Anschutz Medical Campus 3:45 pm Break 4:00 pm Midbody transcriptome and function . Ahna Skop, University of Wisconsin-Madison 4:25 pm Midbody function in morphogenesis . Christian Pohl, Goethe University Medical School 4:50 pm Midbody function in epithelia architecture and polarity . Maja Köhn University of Freiburg, Germany 5:10 pm Midbody formation and abscission in the developing brain . Noelle Dwyer, University of Virginia
The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 41 OO Hit the Ground Running: Early Success in Graduate School
2:00-3:00 pm Room 15A Michael Boyce, Assistant Professor of Biochemistry, Duke University, School of Medicine (co-organizer) Ben Clarke, Associate Professor of Biomedical Sciences, University of Minnesota, Duluth (co-organizer) Shaimar Gonzales, Graduate Student, University of Texas Health Science Center at San Antonio Marina Holz, Dean, Graduate School of Basic Medical Sciences, New York Medical College Jeannette Huaman, Graduate Student, The Graduate Center, City University of New York Kwabena Badu-Nkansah, Department of Cell Biology, Duke University
This highly interactive panel discussion will help attendees improve the knowledge and skills needed to succeed in the first two to three years of graduate school . Panelists will discuss considerations such as choosing a research lab, advisor, and project; bolstering scientific and professional confidence; early-stage strategies for long-term professional success; integrating into local and national scientific and academic communities; and work-life balance . Panelists include both current faculty and senior graduate students in order to provide a range of perspectives on early graduate education . After brief introductory remarks by the panelists, the session will be driven by audience questions and discussion .
Outcomes: 1 . Discuss the skills needed to succeed in the first two to three years of graduate school . 2 . Receive tips and advice on such topics as: choosing a lab/advisor/project, integrating into the academic community, coping with stress . 3 . Learn strategies to build scientific professional skills and confidence . 4 . Engage in an interactive Q&A session driven by attendee interests .
Target audience: advanced undergraduates and early-stage graduate students
OO Judged Poster Session
3:15-5:45 pm Room 16A Supported by Burroughs Wellcome Fund
The Minorities Affairs Committee (MAC) in partnership with the Education Committee offer a judged poster session for MAC travel grant awardees and undergraduate authors on abstracts . At this event postdoctoral, graduate, and undergraduate posters are judged by volunteer faculty and postdocs . This event is an opportunity for networking between our diverse and up-and-coming ASCB meeting attendees and the membership at large . The experience offers professional development opportunities for presenters and professional service opportunities for poster judges .
Outcomes: 1 . Communicate your laboratory findings with a diverse group of peers and more senior cell biologists attending ASCB from around the world . 2 . Demonstrate an understanding of the processes involved in the generation of new knowledge, including the scientific method, data collection and analysis . 3 . Demonstrate the ability to ask and respond to questions about your research .
Target audience: all MAC travel awardees, undergraduate authors on abstracts, all attendees
42 The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org OO Keynote Lecture: Sean Morrison
6:00 pm Ballroom 20B SATURDAY Supported by the Allen Institute for Cell Science Niches for Stem Cells in Bone Marrow SATURDAY
Sean J. Morrison, Director, Children’s Medical Center Research Institute, University of Texas Southwestern Medical Center/HHMI
OO Opening Night Reception
Immediately Following Keynote-10:00 pm Sails Pavilion Join us in celebrating the start of another great meeting! Meet new people, find old friends and colleagues, and start having fun . All registered meeting attendees and exhibitors are invited to the buffet reception . Cash bar available .
OO International Research and Training Exchange Fair
8:00-9:00 pm Sails Pavilion Coordinator: Xuebiao Yao, University of Science & Technology of China
As a feature of the Opening Night Reception, the fair will allow attendees to learn about research, training, and other opportunities in countries around the world; encourage students and postdocs to think about possibilities in other countries; and open up exchanges between labs for international collaboration . Tables will be set up displaying information from various countries and regions around the world, and representatives will be available to answer questions . Make sure to check out this event while you enjoy refreshments and collegiality during the Opening Night Reception!
Target audience: all ASCB attendees interested in scientific opportunities around the world
The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org 43 NOTES
44 The 2018 ASCB | EMBO Meeting l ascb-embo2018.ascb.org