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STUDIES OF GANODERMA LUCIDUM AND GANODERMA TSUGAE (DELIGNIFICATION, MATING SYSTEMS, ROOT ROT, CULTURAL MORPHOLOGY, TAXONOMY). Item Type text; Dissertation-Reproduction (electronic) Authors ADASKAVEG, JAMES ELLIOTT. Publisher The University of Arizona. Rights Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. Download date 07/10/2021 08:17:15 Link to Item http://hdl.handle.net/10150/188172 INFORMATION TO USERS This reproduction was made from a copy of a manuscript sent to us for publication and microfilming. While the most advanced technology has been used to pho tograph and reproduce this manuscript. the quality of the reproduction is heavily dependent upon the quality of the material submitted. 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For an additional charge. all photographs are available in black and white standard 35mm slide format. * *For more information about black and white slides or enlarged paper reproductions. please contact the Dissertations Customer Services Department. Dissertation UMIII .. Information Service University Microfilms International A Bell & Howell Information Company . 300 N. Zeeb Road, Ann Arbor, Michigan 48106 8613803 Adaskaveg, James Elliott STUDIES OF GANODERMA LUCIDUM AND GANODERMA TSUGAE The University of Arizona PH.D. 1986 University Microfilms International 300 N. Zeeb Road, Ann Arbor, MI4810S PLEASE NOTE: In all cases this material has been filmed in the best possible way from the available copy. Problems encountered with this document have been identified here with a check mark_";_. 1. Glossy photographs or pages L 2. Colored illustrations, paper or print ___ 3. Photographs with dark background V 4. Illustrations are poor copy ___ 5. 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Other___________________________ ___ University Microfilms International STUDIES OF GANODERMA LUCIDUM AND GANODERMA TSUGAE by James Elliott Adaskaveg A Dissertation Submitted to the Faculty of the DEPARTMENT OF PLANT PATHOLOGY In Partial Fullment of the Requirements For the Degree of DOCTOR OF PHILOSOPHY In the Graduate College THE UNIVERSITY OF ARIZONA 1 986 THE UNIVERSITY OF ARIZONA GRADUATE COLLEGE As members of the Final Examination Committee, we certify that we have read James Elliott Adaskaveg the dissertation prepared by Studies of Ganoderma lucidum and Ganoderma tsugae entitled ________________~======~=====: ____ ========~==-==~ ____ ___ and recommend that it be accepted as fulfilling the dissertation requirement Doctor of Philosophy for the Degree of --------------------------------------------------------- ~Ji~ Date Date Date Date Date Final approval and acceptance of this dissertation is contingent upon the candidate's submission of the final copy of the dissertation to the Graduate College. I hereby certify that I have read this dissertation prepared under my direction and recommend that it be accepted as fulfilling the dissertation re£:;:P~ Dissertation Director Date STATEMENT BY THE AUTHOR This dissertation has been submitted in partial fulfillment of requirements for an advanced degree at The University of Arizona and is deposited in the University Library to be made available to borrowers under rules of the Library. Brief quotations from this dissertation are allowable without special permission, provided that accurate acknowledgement of source is made. Requests for permission for extended quotation from or reproduction of this manuscript in whole or in part may be granted by the head of the major department or the Dean of the Graduate College when in his or her judgement the proposed use of the material is in the interests of scholarship. In all other instances, however, permission must be obtained from the author. DEDICATION I would like to dedicate this work in memory of a very special uncle, Michael Venditto, who inspired my agricultural interests and to my mother and father, Fannie and Alex Adaskaveg, whose love was always felt and whose personal sacrifices, constant support, and faith have allowed me to follow my interests and obtain my goals. Although my appreciation was not often spoken it was always heartfelt. Thank you ever so much. ii i ACKNOWLEDGMENTS The author wishes to express sincere graditude to the University of Arizona for providing facilities and financial support for this research. I wish especially to thank: Dr. Robert L. Gilbertson for his supervision and advice during these studies ~ut most of all for his consistent helpfulness, stimulating interest, patience, and great friendship; Dr. Richard B. Hine for his constant support and interest in my behalf and for his kind friendship; and Dr. George B. Cummins for his words of wisdom and sincere friendship. I would also like to thank: Dr. M. Nelson for his support and interest in this research; Dr. M.E. Stanghell ini, Dr. M.A. McClure, Dr. S.M. Alcorn, and Dr. I. Misaghi for thei r research advice and assistance, support and interest, for thei r critical revie~is of my work, and for their generosity in providing countless resources; Dr. M. Blackwell (Louisiana State University) for her GanodermA collections, literature searches, and unending support and interest; Dr. P.J. Cotty for his technical advice with chemical analyses; Dr. J. Mihail for her critical reviews and advice in statistical analyses; David Bentley for his personal help and support in providing resources and expertise in electron microscopy and photography; Tom Kruk for his critical reviews; Dr. G. Dutt for obtaining grape wood; and Dr. E.A. Mielke for use of field grape plants. Further thanks to Bobbie Malandrone in her memory; Majorie Braun for all her hel p in the office; Dr. K.H. Yohem for her Ganoderm.ll collections; Plant Pathology Department members for many happy iv v memories; and for all mentioned their friendship. I would like to also thank family members: Michael and Maryellen for the photographic equipment and all the phone calls that brightened my day; Alex and Debbie for their support and encouragement; Keith W. Leahy, who was more than a great cousin but a sincere and heartful friend whose inpromt collecting of Ganoderma specimens helped me significantly; and Frank and Julia Venditto for their personal sacrifices and inspiration. Most of all I would like to express my greatest thanks to Peg Mauk for all her personal sacrifices, continuing and unending assistance in all aspects of my research, and most sincerely for her steadfast faith and boundless love in standing by me in toubled and triumphant times. TABLE OF CONTENTS Page LIST OF TABLES viii LIST OF ILLUSTRATIONS • • ix ABSTRACT xi 1. CULTURAL STUDIES AND GENETICS OF SEXUALITY OF GANODERMA LUClPUM AND ~ TSUGAE IN RELATION TO THE TAXONOMY OF THE ~ LUCIPUM COMPLEX • • • • • • • • • • • • 1 Introduction • • • • • • • • • • • • • • • • • • 1 Materials and Methods •••••••••••••• 5 fruiting Body Collections and Descriptions •••• 5 Macroscopic Cultural and Temperature Studies 5 Microscopic Cultural and Basidiospore Morphology Studies ................ 7 Isolation of Homokaryons, Mating Systems, and Interfertility Studies ••••••••• 8 Results •• • • • • • • • • • • . • • • • . • 9 fruiting Body Descriptions •••••••••••• 9 Macroscopic Cultural and Temperature Studies ••• 10 Microscopic Cultural and Basidiospore Morphology Stud 1es • • • • • • • • • • • • • • • • • • • • • • 14 Mating Systems and Interfertility Studies •• 17 Discussion • • • • • • • • • • • • • • • • • • • • • • • 19 2. IN Y1IBQ DECAY STUDIES Of SELECTIVE DELIGNlfICATION AND SIMULTANEOUS DECAY BY THE WHITE-ROT fUNGI GANOpERMA LUCIDUM AND .G.... TSUGAE • • • • • • • • • • • • • • • 26 Introduction • • • • • • • • • • • • • • • • • 26 Materials and Methods •••••••• 28 fungal Isolates and Wood Samples 28 In ~ Wood Decay Studies ••• 28 Chemical Analyses of Wood Blocks 29 Microscopic Observations ••••• 30 Results ••••••••.•••••••

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