Biology and Diversity of Desmids

Total Page:16

File Type:pdf, Size:1020Kb

Biology and Diversity of Desmids Palacký University, Faculty of Science Department of Botany Biology and diversity of desmids Petra Mazalová Ph.D. thesis Olomouc, 2013 Supervised by: Prof. RNDr. Aloisie Poulíčková, CSc., Department of Botany, Palacký University I hereby declare that this thesis is my independent work, using the listed references, or in co- operation with co-authors of the papers. I did not submit any of the theses or their any part to acquire any other academic title. April 2013, Olomouc ................................. Mgr. Petra Mazalová Motto: „Only nature knows what it wants. It never makes jokes and never makes mistakes. Those make only men.” „In nature we never see anything isolated, but everything in connection with something else which is before it, beside it, under it and over it.” (Johann Wolfgang von Goethe) (Staurodesmus convergens; foto: P. Mazalová) Acknowledgements I would like to thank my supervisor, Aloisie Poulíčková, for her systematic guidance, collaboration, support and inspiration. I also express my appreciation to colleagues from our algological laboratory for their contribution to a good working atmosphere and for helpful advice whenever I needed it. Finally I kindly thank my family for their unwavering support throughout my life. Abstract Higher plants evolved from algae, and still share many common characteristics. However, studies on biology and diversity of algae lag behind those of higher plants. In this thesis, I have investigated the phylogenetic, morphological and geographical diversity of desmids and explored the DNA content variations for better understanding of the importance of polyploidy in their evolution. In addition, I propose there, a multi-step protocol that leads to the quantification of DNA content in desmids and some other microalgae using flow cytometry. In the course of the study of nine mires in Central and Northern Moravia, altogether 109 desmid taxa were found. Five of those taxa are considered to be new records for the Czech Republic, 42 of them are new records for Moravia. The findings were discussed with previous records from Moravia and Czech Republic. Moreover, the character and origin of the unique locality Slavkov mire were discussed. The results indicate a lack of knowledge of desmid diversity in the Moravia region. A pair of well-known desmid species, Micrasterias rotata and M. fimbriata, were tested for their phylogenetic and morphological homogeneity as well as their geographic distribution. The investigation was based on morphological and geometric morphometric studies, with genetic analyses of the trnGucc sequences of strains, isolated mainly from Europe. Micrasterias rotata proved to be phylogenetically homogenous across Europe, while M. fimbriata turned out to be composed of two firmly delimited lineages. The lineages differ genetically as well as in their morphology. There are also differences in their geographic distribution patterns and likely in their ecological preferences. It has been suggested to describe one lineage as a new species of the genus Micrasterias. Nuclear DNA content was measured in microalgae with a focus on desmids, using a new protocol based on flow cytometry measurements. To obtain the protoplasts, a mixture of enzymes (cellulase and macerozyme) was used. The nuclei suspension was prepared using LB01 nuclear isolation buffer, and nuclei were stained by propidium iodide. The leaves of four plant species were used as standards, and one new microalgal standard was suggested. The protocol proved to be useful for desmids and also worked for some Chlorophyta and Heterokontophyta. Optimization of enzyme mixture or nuclear isolation buffer for measuring other microalgae, as in those we did not succeed in measuring, deserves more attention. The nuclear DNA content of 34 Micrasterias strains (25 Micrasterias species) was measured. Intraspecific and interspecific variation was observed and estimated DNA contents were mapped along the phylogenetic tree, based on concatenated SSU rDNA, psaA, and coxIII alignment. The related species were mostly similar in their DNA content. Strong correlation between the absolute nuclear DNA content and chromosome numbers in species, for which the data were available, was found. Significant positive correlation between the DNA content and cell size of the investigated Micrasterias rotata strains was also found. In conclusion the evolutionary significance of genome size variation was confirmed in the genus Micrasterias and the importance of natural polyploidization in the evolution and speciation of it was verified. Abstrakt Vznik a vývoj vyšších rostlin úzce souvisí s řasami, vyšší rostliny s řasami doposud sdílejí mnoho společných znaků. Nicméně studie biologie a diverzity řas pokulhávají za rozsáhlostí studií vyšších rostlin. V této disertační práci byly zkoumány fylogenetická, morfologická a geografická diverzita krásivek a rozdíly v množství jejich jaderné DNA, za účelem lepšího pochopení významu polyploidie v jejich evoluci. Navíc zde navrhuji protokol pro měření množství jaderné DNA u krásivek a několika dalších mikrořas, a to pomocí průtokové cytometrie. Během studia devíti mokřadních lokalit střední a severní Moravy bylo nalezeno celkem 109 taxonů krásivek. Pět z těchto taxonů je nových pro území ČR a 42 pro území Moravy. Byly diskutovány předchozí nálezy všech taxonů na území Moravy či celé České republiky. Zároveň byl zvážen charakter a původ unikátní lokality nacházející se poblíž vesnice Slavkov. Získaná data naznačila nízkou prozkoumanost krásivek na území Moravy. Dva dobře známé druhy krásivek, Micrasterias rotata a M. fimbriata, byly zkoumány z pohledu jejich fylogenetické a morfologické homogenity a geografického rozšíření. Výzkum byl proveden pomocí morfologických a morfometrických analýz, spolu s fylogenetickou analýzou trnGucc kmenů z různých lokalit Evropy. Micrasterias rotata se ukázala být fylogeneticky homogenní v rámci Evropy, zatímco M. fimbriata se rozdělila do dvou dobře podpořených linií. Populace těchto linií se lišily kromě fylogenetické analýzy také morfologickými a morfometrickými daty. Byly zjištěny rozdíly v jejich geografickém rozšíření a pravděpodobně rozdílné ekologické nároky. Na základě výsledků bylo navrženo popsat jednu z těchto linií jako nový druh rodu Micrasterias. Pomocí průtokové cytometrie bylo měřeno množství jaderné DNA u krásivek a několika dalších mikrořas. Byl použit nový protokol vzniklý na základě poznatků z měření u vyšších rostlin. Pro získání protoplastů byla použita směs enzymů celulázy a mecerozymu. Suspenze jader byla získána použitím LB01 lyzačního pufru a následně byla jádra obarvena propidium jodidem. Jako standard byly použity listy čtyř různých rostlin a byl navržen nový standard z řad mikrořas. Protokol se ukázal vhodný pro použití u krásivek a fungoval i u několika zástupců z řad Chlorophyta a Heterokontophyta. Optimalizace enzymatické směsi či lyzačního pufru pro jiné skupiny mikrořas, a pro ty, u kterých nebyla metoda úspěšná, si zaslouží další pozornost. U 34 kultur (25 druhů) rodu Micrasterias bylo změřeno množství jaderné DNA. Byla sledována mezidruhová a vnitrodruhová variabilita tohoto množství a zjištěné hodnoty DNA byly namodelovány na fylogenetický strom založený na propojení analýz SSU rDNA, psaA a coxIII. Příbuzné druhy vykázaly podobná množství DNA. U druhů, pro které byla známa data o počtu chromozomů, byla zjištěna silná pozitivní korelace mezi množstvím DNA a počtem chromozomů. Pozitivní korelace se projevila i mezi množstvím DNA a rozměry buněk u sledovaného druhu Micrasterias rotata. Experimenty poskytly důkaz významu změny velikosti genomu v evoluci rodu Micrasterias a potvrdily důležitost přirozené polyploidizace v jeho evoluci a speciaci. Contents 1 Introduction .................................................................................................................... 10 1.1 Systematic delimitation of desmids............................................................................. 10 1.1.1 Position of desmids within Plantae ......................................................................... 10 1.1.2 Desmid classification .............................................................................................. 11 1.2 Basic characteristics of desmids: an outline .............................................................. 12 1.2.1 Main morphological features of desmids................................................................ 12 1.2.2 Reproduction of desmids ......................................................................................... 13 1.2.3 Ecological preferences of desmids.......................................................................... 14 1.2.4 Genus Micrasterias ................................................................................................. 14 1.3 Polyphyletism and cryptic diversity within desmids................................................. 15 2 Aims of the thesis............................................................................................................ 17 3 Conclusions and future prospects................................................................................. 18 3.1 Desmid flora of mires in Central and Northern Moravia ........................................ 18 3.2 Delimitation of Micrasterias fimbriata and M. rotata ................................................ 19 3.3 Quantification of DNA content in microalgae using flow cytometry ...................... 20 3.4 DNA content in evolution of the genus Micrasterias ................................................
Recommended publications
  • Universidad Autónoma De Nuevo León Facultad De Ciencias Biológicas
    UNIVERSIDAD AUTÓNOMA DE NUEVO LEÓN FACULTAD DE CIENCIAS BIOLÓGICAS TESIS TAXONOMÍA, DISTRIBUCIÓN E IMPORTANCIA DE LAS ALGAS DE NUEVO LEÓN POR DIANA ELENA AGUIRRE CAVAZOS COMO REQUISITO PARCIAL PARA OBTENER EL GRADO DE DOCTOR EN CIENCIAS CON ACENTUACIÓN EN MANEJO Y ADMINISTRACIÓN DE RECURSOS VEGETALES MAYO, 2018 TAXONOMÍA, DISTRIBUCIÓN E IMPORTANCIA DE LAS ALGAS DE NUEVO LEÓN Comité de Tesis Presidente: Dr. Sergio Manuel Salcedo Martínez. Secretario: Dr. Sergio Moreno Limón. Vocal 1: Hugo Alberto Luna Olvera. Vocal 2: Dr. Marco Antonio Alvarado Vázquez. Vocal 3: Dra. Alejandra Rocha Estrada. TAXONOMÍA, DISTRIBUCIÓN E IMPORTANCIA DE LAS ALGAS DE NUEVO LEÓN Dirección de Tesis Director: Dr. Sergio Manuel Salcedo Martínez. AGRADECIMIENTOS A Dios, por guiar siempre mis pasos y darme fortaleza ante las dificultades. Al Dr. Sergio Manuel Salcedo Martínez, por su disposición para participar como director de este proyecto, por sus consejos y enseñanzas que siempre tendré presente tanto en mi vida profesional como personal; pero sobre todo por su dedicación, paciencia y comprensión que hicieron posible la realización de este trabajo. A la Dra. Alejandra Rocha Estrada, El Dr. Marco Antonio Alvarado Vázquez, el Dr. Sergio Moreno Limón y el Dr. Hugo Alberto Luna Olvera por su apoyo y aportaciones para la realización de este trabajo. Al Dr. Eberto Novelo, por sus valiosas aportaciones para enriquecer el listado taxonómico. A la M.C. Cecilia Galicia Campos, gracias Cecy, por hacer tan amena la estancia en el laboratorio y en el Herbario; por esas pláticas interminables y esas “riso terapias” que siempre levantaban el ánimo. A mis entrañables amigos, “los biólogos”, “los cacos”: Brenda, Libe, Lula, Samy, David, Gera, Pancho, Reynaldo y Ricardo.
    [Show full text]
  • Structural Features of Unicellular Desmids (Desmidiales) When Examined in a Scanning Electron Microscope © O
    BOTANICHESKII ZHURNAL, 2021, Vol. 106, N 6, pp. 523–528 COMMUNICATIONS Structural Features of Unicellular Desmids (Desmidiales) when Examined in a Scanning Electron Microscope © O. V. Anissimovaa,# and A. F. Luknitskayab,## a Zvenigorod Biological Station, M.V. Lomonosov Moscow State University Leninskiye Gory, 1/12, Moscow, 119234, Russia b Komarov Botanical Institute RAS Prof. Popov Str., 2, St. Petersburg, 197376, Russia #e-mail: [email protected] ##e-mail: [email protected] DOI: 10.31857/S0006813621060028 We have demonstrated the possibility of using scanning electron microscopy methods for studying the mor- phology and ornamentation of the cell wall of unicellular species of desmid algae (Charophyta, Zygnemato- phyceae). Scanning electron microscopy was used to confirm and refine the identification of taxa with 10 spe- cies as an example: Cosmarium sp., C. anceps, C. granatum, C. nymannianum, C. pokornyanum, Euastrum bidentatum, E. crassicolle, E. luetkemuelleri, E. oblongum, Pleurotaenium ehrenbergii. The use of electron microscope enables a more subtle and qualitative study of the cell wall surface. We con- sidered the difficulties arising when working with cells of desmids in scanning electron microscopy. Attention should be paid to the artifacts arising from the preparation of samples for the study of algae in scanning elec- tron microscopy: mucus plugs and abundant accumulation of mucus on the cell surface, “molting” process and asymmetry in the development of the semicells. Keywords: Сharophyta, Zygnematophyceae, cell wall, morphology, taxonomy, scanning electron microscope ACKNOWLEDGEMENTS Brook A.J. 1981. The biology of desmids. Oxford. 276 p. The studies were carried out within the framework of the Kosinskaya E.K. 1960. Flora sporovykh rasteniy SSSR.
    [Show full text]
  • Copyrighted Material
    1 Symmetry of Shapes in Biology: from D’Arcy Thompson to Morphometrics 1.1. Introduction Any attentive observer of the morphological diversity of the living world quickly becomes convinced of the omnipresence of its multiple symmetries. From unicellular to multicellular organisms, most organic forms present an anatomical or morphological organization that often reflects, with remarkable precision, the expression of geometric principles of symmetry. The bilateral symmetry of lepidopteran wings, the rotational symmetry of starfish and flower corollas, the spiral symmetry of nautilus shells and goat horns, and the translational symmetry of myriapod segments are all eloquent examples (Figure 1.1). Although the harmony that emanates from the symmetry of organic forms has inspired many artists, it has also fascinated generations of biologists wondering about the regulatory principles governing the development of these forms. This is the case for D’Arcy Thompson (1860–1948), for whom the organic expression of symmetries supported his vision of the role of physical forces and mathematical principles in the processes of morphogenesisCOPYRIGHTED and growth. D’Arcy Thompson’s MATERIAL work also foreshadowed the emergence of a science of forms (Gould 1971), one facet of which is a new branch of biometrics, morphometrics, which focuses on the quantitative description of shapes and the statistical analysis of their variations. Over the past two decades, morphometrics has developed a methodological Chapter written by Sylvain GERBER and Yoland SAVRIAMA. 2 Systematics and the Exploration of Life framework for the analysis of symmetry. The study of symmetry is today at the heart of several research programs as an object of study in its own right, or as a property allowing developmental or evolutionary inferences.
    [Show full text]
  • Old Woman Creek National Estuarine Research Reserve Management Plan 2011-2016
    Old Woman Creek National Estuarine Research Reserve Management Plan 2011-2016 April 1981 Revised, May 1982 2nd revision, April 1983 3rd revision, December 1999 4th revision, May 2011 Prepared for U.S. Department of Commerce Ohio Department of Natural Resources National Oceanic and Atmospheric Administration Division of Wildlife Office of Ocean and Coastal Resource Management 2045 Morse Road, Bldg. G Estuarine Reserves Division Columbus, Ohio 1305 East West Highway 43229-6693 Silver Spring, MD 20910 This management plan has been developed in accordance with NOAA regulations, including all provisions for public involvement. It is consistent with the congressional intent of Section 315 of the Coastal Zone Management Act of 1972, as amended, and the provisions of the Ohio Coastal Management Program. OWC NERR Management Plan, 2011 - 2016 Acknowledgements This management plan was prepared by the staff and Advisory Council of the Old Woman Creek National Estuarine Research Reserve (OWC NERR), in collaboration with the Ohio Department of Natural Resources-Division of Wildlife. Participants in the planning process included: Manager, Frank Lopez; Research Coordinator, Dr. David Klarer; Coastal Training Program Coordinator, Heather Elmer; Education Coordinator, Ann Keefe; Education Specialist Phoebe Van Zoest; and Office Assistant, Gloria Pasterak. Other Reserve staff including Dick Boyer and Marje Bernhardt contributed their expertise to numerous planning meetings. The Reserve is grateful for the input and recommendations provided by members of the Old Woman Creek NERR Advisory Council. The Reserve is appreciative of the review, guidance, and council of Division of Wildlife Executive Administrator Dave Scott and the mapping expertise of Keith Lott and the late Steve Barry.
    [Show full text]
  • Gênero Closterium (Closteriaceae) Na Comunidade Perifítica Do Reservatório De Salto Do Vau, Sul Do Brasil
    Gênero Closterium (Closteriaceae) ... 45 Gênero Closterium (Closteriaceae) na comunidade perifítica do Reservatório de Salto do Vau, sul do Brasil Sirlene Aparecida Felisberto & Liliana Rodrigues Universidade Estadual de Maringá, PEA/Nupélia. Av. Colombo, 3790, Maringá, Paraná, Brasil. [email protected] RESUMO – Este trabalho objetivou descrever, ilustrar e registrar a ocorrência de Closterium na comunidade perifítica do reservatório de Salto do Vau. As coletas do perifíton foram realizadas no período de verão e inverno, em 2002, nas regiões superior, intermediária e lacustre do reservatório. Os substratos coletados na região litorânea foram de vegetação aquática, sempre no estádio adulto. Foram registradas 23 espécies pertencentes ao gênero Closterium, com maior número para o período de verão (22) do que para o inverno (11). A maior riqueza de táxons foi registrada na região lacustre do reservatório no verão e na intermediária no inverno. As espécies melhor representadas foram: Closterium ehrenbergii Meneghini ex Ralfs var. immane Wolle, C. incurvum Brébisson var. incurvum e C. moniliferum (Bory) Ehrenberg ex Ralfs var. concavum Klebs. Palavras-chave: taxonomia, Closteriaceae, algas perifíticas, distribuição longitudinal. ABSTRACT – Genus Closterium (Closteriaceae) in periphytic community in Salto do Vau Reservoir, southern Brazil. The aim of this study was to describe, illustrate and to register the occurrence of Closterium in the periphytic community in Salto do Vau reservoir. The samples were collected in the summer and winter periods, during 2002. Samples were taken from natural substratum of the epiphyton type in the adult stadium. Substrata were collected in three regions from the littoral region (superior, intermediate, and lacustrine). In the results there were registered 23 species in the Closterium, with 22 registered in the summer and 11 in the winter period.
    [Show full text]
  • A Study on Chlorophyceae of the Artificial Ponds and Lakes of the National Botanical Garden of Iran
    A STUDY ON CHLOROPHYCEAE OF THE ARTIFICIAL PONDS AND LAKES OF THE NATIONAL BOTANICAL GARDEN OF IRAN T. Nejadsattari, Z. Shariatmadari and Z. Jamzad Nejadsattari, T., Z. Shariatmadari, and Z. Jamzad,.2006 01 01:.A study on chlorophyceae of the artificial ponds and lakes of National Botanical Garden of Iran . – Iran. Journ. Bot. 11 (2): 159-168. Tehran Five aquatic sites of National Botanical Garden of Iran monthly were sampled from December 2003 through November 2004. 68 genera and species of 10 families and 6 orders of the planktonic Chlorophyceae were identified. Among the families Desmidaceae with 22 genera and species showed the highest species richness. Scenedesmaceae (15 species) and Oocystaceae (14 species), Hydrodictyaceae (7 species), Ulotrichaceae (3 species), Zygnemataceae (2 species), Volvocaceae (2 species) and Cladophoraceae, Oedogoniaceae and Trentephliaceae each with 1 species respectively presented in the studied sites. High population densities of species were observed in the warm months. Taher Nejadsattari, Department of Plant Biology, Faculty of Basic Sciences, Islamic Azad University Science and Research Branch, Tehran. –Ziba Jamzad, Reasearch Institute of Forests and Rangelands, P. O. Box 13185- 116, Tehran, Iran. –Zeinab Shariatmadari, Department of Plant biology, Faculty of Basic Sciences, Islamic Azad University Science and Research Branch, Tehran. Keywords. Chlorophyceae, identification, Botanical garden, Iran. ﻣﻄﺎﻟﻌﻪاي در ﻣﻮرد ﺟﻠﺒﻜﻬﺎي ﺳﺒﺰ درﻳﺎﭼﻪﻫﺎ و ﺑﺮﻛﻪﻫﺎي ﺑﺎغ ﮔﻴﺎﻫﺸﻨﺎﺳﻲ ﻣﻠﻲ اﻳﺮان ﻃﺎﻫﺮ ﻧﮋاد ﺳﺘﺎري، زﻳﻨﺐ ﺷﺮﻳﻌﺘﻤﺪاري و زﻳﺒﺎ ﺟﻢ زاد در ﻃﻲ اﻳﻦ ﺗﺤﻘﻴﻖ ﺟﻠﺒﻜﻬﺎي ﺳﺒﺰ 5 ﺑﺮﻛﻪ ﻣﺼﻨﻮﻋﻲ در ﺑﺎغ ﮔﻴﺎﻫﺸﻨﺎﺳﻲ ﻣﻠﻲ اﻳﺮان ﺑﺎ ﻧﻤﻮﻧﻪﺑﺮداري ﻣﺎﻫﻴﺎﻧﻪ از آذر 1382 ﺗﺎ آﺑﺎن 1383 ﻣﻮرد ﻣﻄﺎﻟﻌﻪ و ﺷﻨﺎﺳﺎﻳﻲ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. 68 ﺟﻨﺲ و ﮔﻮﻧﻪ ﻣﺘﻌﻠﻖ ﺑﻪ 10 ﺗﻴﺮه و 6 راﺳﺘﻪ از ﺟﻠﺒﻜﻬﺎي ﺳﺒﺰ ﺷﻨﺎﺳﺎﻳﻲ ﮔﺮدﻳﺪ.
    [Show full text]
  • New Phylogenetic Hypotheses for the Core Chlorophyta Based on Chloroplast Sequence Data
    ORIGINAL RESEARCH ARTICLE published: 17 October 2014 ECOLOGY AND EVOLUTION doi: 10.3389/fevo.2014.00063 New phylogenetic hypotheses for the core Chlorophyta based on chloroplast sequence data Karolina Fucíkovᡠ1, Frederik Leliaert 2,3, Endymion D. Cooper 4, Pavel Škaloud 5, Sofie D’Hondt 2, Olivier De Clerck 2, Carlos F. D. Gurgel 6, Louise A. Lewis 1, Paul O. Lewis 1, Juan M. Lopez-Bautista 3, Charles F. Delwiche 4 and Heroen Verbruggen 7* 1 Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT, USA 2 Phycology Research Group, Biology Department, Ghent University, Ghent, Belgium 3 Department of Biological Sciences, The University of Alabama, Tuscaloosa, AL, USA 4 Department of Cell Biology and Molecular Genetics and the Maryland Agricultural Experiment Station, University of Maryland, College Park, MD, USA 5 Department of Botany, Faculty of Science, Charles University in Prague, Prague, Czech Republic 6 School of Earth and Environmental Sciences, The University of Adelaide, Adelaide, SA, Australia 7 School of Botany, University of Melbourne, Melbourne, VIC, Australia Edited by: Phylogenetic relationships in the green algal phylum Chlorophyta have long been subject to Debashish Bhattacharya, Rutgers, debate, especially at higher taxonomic ranks (order, class). The relationships among three The State University of New Jersey, traditionally defined and well-studied classes, Chlorophyceae, Trebouxiophyceae, and USA Ulvophyceae are of particular interest, as these groups are species-rich and ecologically Reviewed by: Jinling Huang, East Carolina important worldwide. Different phylogenetic hypotheses have been proposed over the University, USA past two decades and the monophyly of the individual classes has been disputed on Cheong Xin Chan, The University of occasion.
    [Show full text]
  • Genus Micrasterias
    Triquetrous forms in the genus Micrasterias by J. Heimans (Amsterdam). In the winter and early spring of 1916 Mrs. Anna Weber-van Bosse at her hospitable residence near Eerbeek initiated me in the study of Freshwater Algae. For several years after that date in numerous trips all over this country I collected and studied some thousands of samples from all kinds The Desmids drew of freshwater ponds and lakes, canals and streams. soon when rich and varied Desmid flora my special attention, an unexpectedly was found in certain fens and ponds in the diluvial and moor districts of our country. considerable of Still more surprising was the presence of a number those Desmid species which in the publications of W. and G. S. West, whose Monograph at that time was the only handbook for the study of Desmids, are held to be confined to the Western rocky districts of the British Isles in the drainage area of precarboniferous rocks. One of the most beautiful and most characteristic species of this "Caledonian type" of Desmid vegetation, Staurastrum Ophiura Lund, had been found by Mrs Weber herself some years before in a sample from the province of North Brabant. did the Not until several years afterwards we learn from publications of R. Gronblad, A. Donat, H. Homfeld and others that this "Atlantic Element of the Desmid flora" is the N.W. of spread over parts Europe from Finland to Portugal. Besides Staurastrum Ophiura a considerable number of species be- longing to this Western element was found in the Netherlands, although of them of Staurastrum brasiliense most are rare occurrence, e.g.
    [Show full text]
  • Desmid of Some Selected Areas of Bangladesh
    Bangladesh J. Plant Taxon. 12(1): 11-23, 2005 (June) DESMIDS OF SOME SELECTED AREAS OF BANGLADESH. 3. DOCIDIUM, PLEUROTAENIUM, TRIPLASTRUM AND TRIPLOCERAS A. K. M. NURUL ISLAM AND NASIMA AKTER Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh Key words: Desmids, Docidium, Pleurotaenium, Triplastrum, Triploceras, Bangladesh Abstract 23 taxa belonging to Pleurotaenium, 2 under Triploceras and 1 each under Docidium and Triplastrum have been recorded in this paper from some selected areas of Bangladesh. Of these, 11 are new records for the country. Introduction This is the third paper in a series under the above title. The first and second papers with the same title have already been published in this journal (Islam and Akter 2004 and Islam and Begum 2004). The present paper includes the species belonging to Docidium, Pleurotaenium, Triplastrum and Triploceras from the same selected areas as mentioned in the above papers. The illustrated descriptions of these taxa are given below. For materials and methods, dates and places of collections and other information see Islam and Akter (2004). Taxonomy Class: Chlorophyceae; Order Desmidiales; Family: Desmidiaceae A total of 27 taxa (Docidium 1, Pleurotaenium 23, Triplastrum 1 and Triploceras 2) have been described with diagrams and photomicrographs. Of these, 11 taxa are new records for the country (marked by *). Genus: Docidium de Brebisson 1844 em. Lundell 1871 Cells straight, cylindrical, smooth, or with undulate margins, 8-26 times longer than broad; circular in cross section, slightly constricted in the midregion, with an open sinus; apex usually truncate, rounded, sometimes dilated, smooth or rarely with a few intramarginal granules; base of semicell inflated, with 6-9 visible folds (plications) at the isthmus, the folds usually subtended by granules; cell wall smooth or faintly punctulate; chloroplast axial with irregular longitudinal ridges and 6-14 axial pyrenoids; zygospore unknown.
    [Show full text]
  • Carbohydrate Release by a Subtropical Strain of Spondylosium Pygmaeum (Zygnematophyceae): Influence of Nitrate Availability and Culture Aging1
    J. Phycol. 46, 477–483 (2010) Ó 2010 Phycological Society of America DOI: 10.1111/j.1529-8817.2010.00823.x CARBOHYDRATE RELEASE BY A SUBTROPICAL STRAIN OF SPONDYLOSIUM PYGMAEUM (ZYGNEMATOPHYCEAE): INFLUENCE OF NITRATE AVAILABILITY AND CULTURE AGING1 Fernanda Reinhardt Piedras Po´s-graduac¸a˜o em Oceanografia Biolo´gica, Instituto de Oceanografia, Universidade Federal de Rio Grande- FURG, Av. Italia, Km 8, Rio Grande, RS 96201-900, Brasil Paulo Roberto Martins Baisch, Maria Isabel Correˆa da Silva Machado Laborato´rio de Oceanografia Geolo´gica Instituto de Oceanografia, Universidade Federal de Rio Grande- FURG, Av. Italia, Km 8, Rio Grande, RS 96201-900, Brasil Armando Augusto Henriques Vieira Departamento de Botanica, Unversidade Federal de Sao Carlos, Via Washington Luis, Km 235, Sao Carlos, SP 13565-905, Brasil and Danilo Giroldo2 Laborato´rio de Botaˆnica Criptogaˆmica, Instituto de Cieˆncias Biolo´gicas, Universidade Federal de Rio Grande – FURG, Av. Italia, Km 8, Rio Grande, RS 96201-900, Brasil This paper describes the influence of nitrate avail- availability. EPS molecules >12 kDa were composed ability on growth and release of dissolved free and mainly of xylose, fucose, and galactose, as for other combined carbohydrates (DFCHOs and DCCHOs) desmids. However, a high N-acetyl-glucosamine con- produced by Spondylosium pygmaeum (Cooke) W. tent was found, uniquely among desmid EPSs. West (Zygnematophyceae). This strain was isolated Key index words: carbohydrate; desmid; growth; from a subtropical shallow pond, located at the nitrate; Spondylosium extreme south of Brazil (Rio Grande, RS). Experi- ments were carried out in batch culture, comparing Abbreviations: Ara, arabinose; DCCHO, dissolved two initial nitrate levels (10 ⁄ 100 lM) in the medium.
    [Show full text]
  • New Desmid Records from High Mountain Lakes in Artabel Lakes Nature Park, Gümüşhane, Turkey
    Turkish Journal of Botany Turk J Bot (2019) 43: 570-583 http://journals.tubitak.gov.tr/botany/ © TÜBİTAK Research Article doi:10.3906/bot-1810-71 New desmid records from high mountain lakes in Artabel Lakes Nature Park, Gümüşhane, Turkey 1, 2 Bülent ŞAHİN *, Bülent AKAR 1 Department of Biology Education, Fatih Education Faculty, Trabzon University, Trabzon, Turkey 2 Department of Food Engineering, Faculty of Engineering and Natural Sciences, Gümüşhane University, Gümüşhane, Turkey Received: 30.10.2018 Accepted/Published Online: 15.04.2019 Final Version: 08.07.2019 Abstract: The algal flora of 17 lakes and 1 pond in the Artabel Lakes Nature Park were investigated during two summer seasons (2013 and 2016). In total, 26 desmid taxa were found and identified as new records for the desmid flora of Turkey based on their morphotaxonomic characteristics and ecological preferences. The taxa identified belong to the genera Actinotaenium (1), Closterium (1), Cosmarium (15), Micrasterias (1), Spondylosium (1), Staurastrum (5), Teilingia (1), and Tetmemorus (1). Morphotaxonomy, ecology, and distribution of each species were discussed in detail. Key words: Desmids, new records, high mountain lakes, Artabel Lakes Nature Park, Turkey 1. Introduction Desmids are an integral part of benthic habitats of Desmid habitats are exclusively freshwater (Coesel and high mountain lakes; in particular, those of the Northern Meesters, 2007; Kouwets, 2008). Desmids usually prefer Hemisphere (Medvedeva, 2001; Sterlyagova, 2008). In acidic or pH-circumneutral, nutrient-poor, and clear the period from 1998 to 2014, 43 new records of desmid waters (Lenzenweger, 1996; Coesel and Meesters, 2007). species from high mountain lakes in the eastern Black It is well known that members of order Desmidiales Sea Region were identified and published (Şahin, 1998, exhibit great diversity in their external morphology and 2000, 2002, 2007, 2008, 2009; Şahin and Akar, 2007; Akar also have remarkably complex cell symmetry (Lee, 2015).
    [Show full text]
  • Some Freshwater Green Algae of Raja-Rani Wetland, Letang, Morang: New for Nepal
    2020J. Pl. Res. Vol. 18, No. 1, pp 6-26, 2020 Journal of Plant Resources Vol.18, No. 1 Some Freshwater Green Algae of Raja-Rani Wetland, Letang, Morang: New for Nepal Shiva Kumar Rai1*, Kalpana Godar1and Sajita Dhakal2 1Phycology Research Lab, Department of Botany, Post Graduate Campus, Tribhuvan University, Biratnagar, Nepal 2National Herbarium and Plant Laboratories, Department of Plant Resources, Godawari, Lalitpur, Nepal *E-mail: [email protected] Abstract Freshwater green alga of Raja-Rani wetland has been studied. A total 36 algal samples were collected from 12 sites by squeezing the submerged aquatic plants. Present paper describes 35 green algae under 18 genera from Raja-Rani wetland as new record for Nepal.Genus Euastrum consists 5 species; genera Cosmarium, Staurodesmus, and Staurastrum consist 4 species each; genera Scenedesmus, Closterium, Pleurotaeniuum and Xanthidium consist 2 species each; and rest genera consist only single taxa each. Water parameters of the wetland of winter, summer and rainy seasons were also recorded. Keywords: Chlorophyceae, Cosmarium, New report, Staurodesmus, Triploceras,Xanthidium Introduction climatic condition and rich aquatic habitats for algae, extensive exploration is lacking in the history.Suxena Algae are the simplest photosynthetic thalloid plants, & Venkateswarlu (1968), Hickel (1973), Joshi usually inhabited in water and moist environment (1979), Subba Raju & Suxena (1979), Shrestha & throughout the world. Green algae are the largest Manandhar (1983), Hirano (1984), Ishida (1986), and most diverse group of algae, with about 8000 Watanabe & Komarek (1988), Haga & Legahri species known (Guiry, 2012). They have wide range (1993), Watanabe (1995), Baral (1996, 1999), Das of habitats as they grow in freshwater, marine, & Verma (1996), Prasad (1996), Komarek & subaerial, terrestrial, epiphytic, endophytic, parasitic, Watanabe (1998), Simkhada et al.
    [Show full text]