Structure and Plasticity of Silent Synapses in Developing

Xu et al. Cell Discovery (2020) 6:8 Cell Discovery https://doi.org/10.1038/s41421-019-0139-1 www.nature.com/celldisc ARTICLE Open Access Structure and plasticity of silent synapses in developing hippocampal neurons visualized by super-resolution imaging Cheng Xu1,2, Hui-Jing Liu2,3,LeiQi2,3, Chang-Lu Tao1,Yu-JianWang1,ZeyuShen2, Chong-Li Tian2,3, Pak-Ming Lau2,3 and Guo-Qiang Bi1,2,4 Abstract Excitatory synapses in the mammalian brain exhibit diverse functional properties in transmission and plasticity. Directly visualizing the structural correlates of such functional heterogeneity is often hindered by the diffraction-limited resolution of conventional optical imaging techniques. Here, we used super-resolution stochastic optical reconstruction microscopy (STORM) to resolve structurally distinct excitatory synapses formed on dendritic shafts and spines. The majority of these shaft synapses contained N-methyl-D-aspartate receptors (NMDARs) but not α-amino-3- hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs), suggesting that they were functionally silent. During development, as more spine synapses formed with increasing sizes and expression of AMPARs and NMDARs, shaft synapses exhibited moderate reduction in density with largely unchanged sizes and receptor expression. Furthermore, upon glycine stimulation to induce chemical long-term potentiation (cLTP), the previously silent shaft synapses became functional shaft synapses by recruiting more AMPARs than did spine synapses. Thus, silent shaft synapse may represent a synaptic state in developing neurons with enhanced capacity of activity-dependent potentiation. 1234567890():,; 1234567890():,; 1234567890():,; 1234567890():,; – Introduction functional form through activity-dependent plasticity13 16. In the mammalian brain, excitatory communication However, the structural and morphological correlates of between neurons is primarily mediated by glutamatergic these functional states have been lacking. Studies with synapses1,2. Activity-induced plasticity of these synapses is electron microscopy (EM) have indicated that most glu- believed to underlie learning and memory function of the tamatergic excitatory synapses are formed on dendritic – brain3 6. Electrophysiological studies have suggested that spines, in contrast to GABAergic inhibitory synapses that excitatory synapses may exhibit distinct functional prop- are primarily formed on dendritic shafts, although erties or states7,8. An extreme case is the so-called silent exceptions have been observed that some excitatory – – synapse9 12, which contains few α-amino-3-hydroxy-5- synapses formed directly on the shafts17 20. With con- methyl-4-isoxazolepropionic acid receptors (AMPARs) ventional fluorescence microscopy, it was observed that and cannot carry out excitatory transmission upon pre- early in development, N-Methyl-D-aspartate receptors synaptic activation, but can be converted into the (NMDARs) clusters might form on dendritic shafts before clustering of AMPARs21. Unfortunately, the diffraction- limited resolution of conventional optical microscopy Correspondence: Pak-Ming Lau ([email protected])or does not allow for unambiguous determination whether Guo-Qiang Bi ([email protected]) 1Hefei National Laboratory for Physical Sciences at the Microscale, University of these receptor clusters are actual shaft synapses. Thus, a Science and Technology of China, Hefei, Anhui 230027, China higher-resolution imaging approach is desired to establish 2 School of Life Sciences, University of Science and Technology of China, Hefei, the link between the morphological and functional states Anhui 230027, China Full list of author information is available at the end of the article. of these synapses. In the current study, we took advantage These authors contributed equally: Cheng Xu, Hui-Jing Liu © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a linktotheCreativeCommons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Xu et al. Cell Discovery (2020) 6:8 Page 2 of 11 of single molecule localization-based super-resolution With STORM imaging, we were able to assess the fluorescence microscopy22,23 and its quantitative cap- expression of AMPARs and NMDARs using the number ability, to investigate in cultured hippocampal neurons the of single molecule localizations as a quantitative measure morphology and receptor expression of different forms of (see Methods)28. Figure 1e, f summarizes the localization excitatory synapses and their changes during development numbers of GluN2B and GluA1 for all putative excitatory and plasticity. synapses identified by vGlut1 puncta from DIV 17 cul- tures. It is clear that most shaft synapses had low AMPAR Results proportion (defined as NGluA1/(NGluA1 + NGluN2B), see In the current study, we used low density culture of rat Methods) and could be classified as “silent synapses”,in hippocampal neurons that formed synaptic connections contrast to the majority of spine synapses that belonged to starting from ~11 days in vitro (DIV). With immuno- the class of “functional” synapses with higher AMPAR fluorescence labeling of presynaptic scaffolding protein proportion (Fig. 1e, f and Supplementary Fig. S5). Notably, bassoon and postsynaptic AMPARs subunit GluA1, many there also existed a relatively small number of spine- synapses were visible under conventional fluorescence shaped silent synapses, consistent with previous obser- microscopy as fluorescent puncta with overlapping bassoon vations using conventional immunofluorescence ima- and GluA1 signals and without much discernable sub- ging21. With 3D STORM, we also observed that for the structures (Fig. 1), because these synapses were usually silent shaft synapses, GluN2B localizations appeared to be hundreds of nanometers in size, close to the diffraction limit primarily on or near the cell surface (Supplementary Fig. of optical microscopy. Thus, it is often hard to determine S6a and Supplementary Movies S1). Similar surface whether a fluorescent punctum near the dendrite is really a expression pattern was also found for GluN2B and GluA1 short spine synapse or a shaft synapse (see also Supple- localizations in dendritic spines (Supplementary Fig. S6b- mentary Fig. S1). Super-resolution stochastic optical d and Supplementary Movies S2). reconstruction microscopy (STORM)24,25 with >10-fold It is known that synapses become enriched in AMPARs improvement in resolution (Supplementary Fig. S2), has during neuronal development and brain maturation21,29. allowed for visualization of finer structural details of these With STORM imaging and analyses, we further evaluated synapses (Fig. 1b). Importantly, with STORM resolution receptor expression in individual synapses at different dendritic GluA1 distribution facilitated visualizing dendritic developmental stages. At DIV11, we found that the profiles (Supplementary Fig. S3), it became much easier to majority of synapses were silent shaft synapses, with a few determine whether a synapse was formed on the spine or spine synapses being either silent (with low AMPAR dendritic shaft (Fig. 1b1, b2 and Supplementary Fig. S1d–f). proportion similar to the silent shaft synapses) or func- From the STORM images, it was clear that a spine synapse tional (with higher AMPAR proportion) (Fig. 2a, d, g and generally contains postsynaptic AMPARs to oppose the Supplementary Fig. S7a). The maturation of the neurons presynaptic bassoon localizations. In contrast, most shaft was accompanied by a moderate decrease in the density synapses contained few AMPARs to oppose bassoon loca- of shaft synapses and a dramatic increase in the density of lizations (Fig. 1b2), although this was often hard to resolve spine synapses (Supplementary Fig. S7). At DIV 16 -17 in the conventional images. and DIV 21-23, the majority of spine synapses contained To determine whether these AMPARs-negative shaft both AMPARs and NMDARs receptors and with high synapses were excitatory silent synapses, we performed AMPAR proportion (Fig. 2b, c, e, f, h, i). In contrast, STORM imaging of NMDARs and AMPARs using anti- although a few shaft synapses contained high levels of bodies against the 2B subunit of NMDARs (GluN2B) and AMPARs (Fig. 2f, i), the majority of shaft synapses at GluA1 containing-AMPARs, respectively, in conjunction these stages were still silent, expressing much fewer with conventional immunofluorescence imaging of vesi- AMPARs as compared to spine synapses (Fig. 2b, c, e, f, h, cular glutamate transporter 1 (vGlut1). Under STORM i). Further analyses revealed that during this period of resolution, many GluN2B positive but GluA1 negative development (from DIV16 -17 to DIV 21-23), there was a puncta were observed with distinct line-shaped structure marked increase in the expression of AMPARs and formed directly along the dendritic shaft (Fig.

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