Construction of a Microsatellites‐Based Linkage Map for the White ( aeneus)

Lior Dor*†, Andrey Shirak*, Sergei Gorshkov‡, Mark R. Band§, Abraham Korol**, Yefim Ronin**, Arie Curzon*, Gideon Hulata*, Eyal Seroussi*and Micha Ron*1

*Institute of Science, Agricultural Research Organization, Bet Dagan 50250, Israel †Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel ‡National Center for Mariculture, Israel Oceanographic and Limnological Research, Eilat 88112, Israel §The Carver Biotechnology Center, University of Illinois, Urbana, IL 61801, USA **University Haifa, Faculty of Science, Institute of Evolution, Haifa 31905, Israel 1Corresponding author

Raw sequencing data of White grouper from this article was deposited under GenBank accession number PRJEB5936

Corresponding author details: Micha Ron; Institute of Animal Science, Agricultural Research Organization, Bet Dagan 50250, Israel; +97289484442 [email protected]

DOI: 10.1534/g3.114.011387

Figure S1 Genotyping by fragment analysis is illustrated for D078 microsatellite marker; heterozygous sire (188/196) and dam (188/192), and the four possible genetic combinations resulting in their progeny. Automatic genotyping is based on fixed positions of three bins (grey fields) representing the three alleles.

2 SI L. Dor et al.

Figure S2 Exclusion of parenthood by number of markers.

L. Dor et al. 3 SI

Table S1 Markers used for construction of tilapia linkage maps.

Available for download as an Excel file at http://www.g3journal.org/lookup/suppl/doi:10.1534/g3.114.011387/‐/DC1

4 SI L. Dor et al.

Table S2 Number of offspring of two males and two females in two subsequent spawns as verified by parenthood identification using 34 microsatellite markers.

Male M2 M4

Spawn 1st 2nd 1st 2nd

F9 37 15 5 0 Female F11 6 14 0 3

L. Dor et al. 5 SI

Table S3 Origin of microsatellite markers used for linkage map construction. Origin of microsatellite markers No.

Heterologous1 40

Largest scaffolds 177 Next generation sequencing TERRA containing scaffolds2 11

Total 228

1Dor et al. (2014) 2Telomeric repeat‐containing RNA (TERRA) (Lejnine et al. 1995).

6 SI L. Dor et al.