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DNA Barcodes of Caulerpa Species (Caulerpaceae, Chlorophyta) from the Northern Philippines

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DNA Barcodes of Caulerpa Species (Caulerpaceae, Chlorophyta) from the Northern Philippines Philippine Journal of Science 148 (2): 337-347, June 2019 ISSN 0031 - 7683 Date Received: 01 Feb 2019 DNA Barcodes of Caulerpa Species (Caulerpaceae, Chlorophyta) from the Northern Philippines Richard V. Dumilag1*, Zae-Zae A. Aguinaldo1,2, Ver Angelo M. Alcoriza1, Maria Pia Sarah B. Balucanag1, Allissa Rose T. Dulalia1, and Arnel R. Sayasa1 1Department of Biological Sciences, Institute of Arts and Sciences Far Eastern University, Nicanor Reyes Sr. St., Sampaloc 1015 Manila, Philippines 2Marine Genomics and Molecular Genetics Laboratory, Marine Science Institute College of Science, University of the Philippines, Diliman 1101 Quezon City, Philippines Species discrimination in Caulerpa – a seaweed genus of economic importance – can be difficult because its members are often morphologically variable, occasionally with some morphological overlap between species. One of the most common approaches to circumvent this challenge is the integration of phenotypic data with information gained from short reference DNA sequences or DNA barcodes. Previous investigations based on this approach have incorporated only a limited number of specimens of Caulerpa from the Philippines. The present study aimed to identify Caulerpa collections from the northern Philippines aided with DNA barcodes. TufA gene sequence data of recent intertidal and shallow subtidal collections confirmed the presence of seven genetically recognized Caulerpa species in the northern Philippines, namely C. chemnitzia, C. cupressoides, C. elongata, C. oligophylla, C. racemosa, C. serrulata, and C. sertularioides. Caulerpa elongata and C. oligophylla were confirmed occurring in the area for the first time. With the addition of species recorded previously but not found during the present study, this brings the total number of Caulerpa species in the region to 15 species. Keywords: Caulerpa elongata, Caulerpa oligophylla, DNA barcoding, taxonomy, tufA gene INTRODUCTION et al. 1972, Kuenen and Debrot 1995, Crockett and Keough 2014). Caulerpa is a genus of marine green algae comprising 97 currently recognized species (Guiry and Guiry 2018). A significant number of Caulerpa species are characterized The genus is pantropical in distribution but also extends by an erect branch forming the central axis (rachis) with into temperate waters. Its members often form turf branchlets (ramuli), collectively forming the assimilator. communities in the shallow intertidal (Belton et al. 2015) The primary criteria for species discrimination include the to depths of up to 100 m (Belsher and Meinesz 1995). branching of the assimilator and shape of the ramuli. These Caulerpa is predominantly an Old World assemblage, morphological traits, however, can exhibit a high degree having so far the highest species diversity in Australia of variation under different environmental conditions (Price 2011, Belton et al. 2019). Despite the occurrence (Peterson 1972, Calvert 1976, Ohba and Enomoto 1987, of several invasive pest taxa (Meinesz 2002, Klein and Ohba et al. 1992, Gacia et al. 1996, Riosmena-Rodríguez Verlaque 2008), species of Caulerpa can constitute et al. 2014) and their convergence in classical character- important components within their native ranges (Alcala systems has led to considerable taxonomic confusion (e.g., Calvert et al. 1976, Coppejans and Prud’homme van *Corresponding author: [email protected] 337 Philippine Journal of Science Dumilag et al.: Northern Philippine Caulerpa species Vol. 148 No. 2, June 2019 Reine 1992, Prud’homme van Reine et al. 1996). Analysis Batanes (Fig. 1). Going under the most popular local of short DNA sequences – referred to as DNA barcodes name, “Ar-arusip,” Caulerpa holds a significant place as – has been widely adopted by phycologists, enabling a traditional food in the region (Blanco 1938, Moreland them to identify unknown specimens in reference to the established classification (Saunders and Kucera 2010, Pasha 2016). Today, species discrimination in Caulerpa is generally based on a number of genomic loci, with the chloroplast gene tufA encoding elongation factor TU being used almost universally (Famà et al. 2002; de Senerpont Domis et al. 2003; Stam et al. 2006; Kazi et al. 2013; Sauvage et al. 2013; Belton et al. 2014, 2015, 2019; Draisma et al. 2014; Wang et al. 2015; Fernández- García et al. 2016). Accordingly, Belton et al. (2014, 2019) proposed tufA DNA barcodes (i.e., reference sequences) for 37 Caulerpa species and analyzed them using a single-locus species delimitation method (General Mixed Yule Coalescent or GMYC) together with other statistical criteria. When possible, they chose the tufA sequences of specimens nearest to the type location as barcode to represent the species. They also advised to abandon the use of infraspecific ranks (variety or form) and use terminology without formal taxonomic assignment (ecads). However, other entities might still show consistent morphological and genetic distinctions – for example, Caulerpa taxifolia var. distichophylla from Caulerpa taxifolia var. taxifolia – in its invasive habitat (Jongma et al. 2013). One of the Philippine hotspots of seaweed diversity is its northern territory (Cordero 1977a, Trono 1998). The area includes the provinces of Ilocos Norte and Cagayan, which respectively encompass the northeast and northwest areas of Luzon Island. Its extreme northern boundary extends across the volcanic island series of Calayan and Figure 1. Map showing the geographic distribution of Caulerpa species reported in the northern Philippines. Collection sites are written in boldface. Figure 2. Gathering and collection of Caulerpa racemosa (“Ar-arusip”) in Sitio, Daisy, Paayas, Burgos, Ilocos Norte, northern Philippines. (A) A local gatherer cleaning Caulerpa produce near shore on a low tide. (B) Habit of C. racemosa showing its typical spherical ramuli (inset), scale = 2 mm. 338 Philippine Journal of Science Dumilag et al.: Northern Philippine Caulerpa species Vol. 148 No. 2, June 2019 Table 1. List of species and distribution of Caulerpa reported from the northern Philippines. Species names are updated from the original publication. The currently accepted names are based on Algaebase (Guiry and Guiry 2018), an international standard database for algal taxonomy, nomenclature, and distribution information. Species Northern Philippine distributiona Reference Caulerpa chemnitzia (Esper) J.V.Lamouroux Cagayan – Babuyan Is.; Ilocos Norte – Burgos, Gilbert 1942bc, Cordero 1983b, Marcos- Pasuquin; Cagayan – Camiguin de Babuyanes Agngarayngay 1983bb, This study Is., Calayan Is. Caulerpa cupressoides (Vahl) C.Agardh Batanes – Batan Is.; Cagayan –Camiguin de Cordero 1983, Marcos-Agngarayngay Babuyanes, Claveria, Sta. Ana; Ilocos Norte 1983a; This study – Burgos var. lycopodium Weber-van Bosse Ilocos Norte – Currimao Hurtado-Ponce 1983a, Hurtado-Ponce and Modelo 1983 var. elegans (P.Crouan & H.Crouan) Batanes – Batan Is. Cordero 1977b Børgesen var. turbinata (J.Agardh) Fernández- Ilocos Norte – Burgos Belleza and Liao 2007c García & Riosmena-Rodríguez Caulerpa elongata Weber-van Bosse Batanes – Batan Is.; Cagayan –Camiguin de This study Babuyanes Is.; Ilocos Norte – Burgos Caulerpa fergusonii Murray Ilocos Norte – Currimao Hurtado-Ponce 1983a,b; Hurtado-Ponce and Modelo 1983; Tungpalan 1983 Caulerpa lentillifera J.Agardh Ilocos Norte – unstated locality Moreland 1979, Agngarayngay et al. 2005 Caulerpa macrodisca Decaisne Cagayan – unstated locality; Ilocos region – Zaneveld 1956e probably in an unstated locality in Ilocos Norte Caulerpa nummularia Harvey ex J.Agardh Batanes – Batan Is. Cordero 1976f Caulerpa oligophylla Montagne Cagayan – Sta. Ana This study Caulerpa parvifolia Harvey Cagayan – Babuyan Is. Gilbert 1942g Caulerpa racemosa (Forsskål) J.Agardh Batanes – Batan Is.; Cagayan –Camiguin de Blanco 1938, Zaneveld 1956, Moreland Babuyanes Is., Sta. Ana, Sta. Praxedes; Ilocos 1979, Marcos-Agngarayngay 1983a,b; Norte –Badoc, Burgos, Currimao, Pagudpud, Tungpalan, 1983; Agngarayngay et al. Pasuquin, Paoay 2005; This study var. clavifera (Turner) Weber-van Bosse Cagayan – Babuyan Is. Gilbert 1942 Caulerpa serrulata (Forsskål) J.Agardh Batanes – Batan Is.; Cagayan –Camiguin de Blanco 1938; Gilbert 1942; Cordero 1983; Babuyanes, Claveria, Gonzaga, Sta. Ana; Hurtado-Ponce 1983a: Hurtado-Ponce Ilocos Norte –Burgos, Currimao & Modelo 1983; Marcos-Agngarayngay 1983a,b; This study var. boryana (J.Agardh) Gilbert f. Ilocos Norte – Currimao Gilbert 1942; Hurtado-Ponce and Modelo longifolia Gilbert 1983 f. lata (Weber-van Bosse) Tseng Ilocos Norte – unstated locality Gilbert 1942 var. serrulata Ilocos Norte – Burgos Belleza and Liao 2007 Caulerpa sertularioides (S.G.Gmelin) Howe Cagayan – Gonzaga; Ilocos Norte –Burgos, Blanco 1938; Zaneveld 1956; Cordero Currimao 1983; Hurtado-Ponce 1983a; Hurtado- Ponce and Modelo 1983; Marcos- Agngarayngay 1983a,b; This study f. brevipes (J.Agardh) Svedelius Batanes – Batan Is. Cordero 1977b f. longiseta (Bory de Saint-Vincent) Ilocos Norte – unstated locality Gilbert 1942 Svedelius Caulerpa subserrata Okamura Ilocos Norte – Burgos, Currimao Cordero 1980; Hurtado-Ponce 1983a: Hurtado-Ponce and Modelo 1983; Marcos-Agngarayngay 1983a Caulerpa taxifolia (Vahl) C. Agardh Batanes – Batan Is.; Ilocos Norte –Burgos Cordero 1977b; Cordero 1983 Caulerpa webbiana Montagne Ilocos Norte – Burgos Cordero 1983 var. pickeringii (Harvey & Bailey) Eubank Ilocos Norte – Burgos Trono 2004 arefer to Fig. 2; bnamed as C. peltata; cnamed as C. peltata var. peltata; dnamed as C. racemosa var. turbinata; enamed
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