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Study on genetic diversity of polyphylla population from and

Article in Cell Biotechnology and Molecular Biology · January 2016

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STUDY ON GENETIC DIVERSITY OF Paris polyphylla POPULATION FROM VIETNAM AND CHINA

HOANG TAN QUANG *, CAI THI QUYNH THOA, TRINH HUU TAN, NGUYEN THANH GIANG, NGUYEN DUC HUY, TRUONG THI BICH PHUONG AND VAN THI YEN Department of Molecular Biology, Institute of Biotechnology, Hue University, (Ngoc Anh, Phu Thuong, Phu Vang, Thua Thien Hue 47000) Vietnam [HTQ, THT, NTG, NDH]. College of Sciences, Hue University, Vietnam [CTQT, TTBP]. College of Agriculture and Forestry, Hue University, Vietnam [VTY]. [*For Correspondence: E-mail: [email protected]]

ABSTRACT We investigated genetic diversity of 55 samples of valuable medicinal plant Paris polyphylla from different ecology regions (Thua Thien Hue, Vinh Phuc, Lao Cai (Vietnam) and Jilin (China)) using RAPD technique. We obtained total of 125 DNA polymorphic bands which average 15.63 polymorphic bands each primer used. The amplicon each primer varied 9-23 bands and their sizes ranged 257 to 2,448 bp. Among four regions, Thua Thien Hue showed highest genetic diversity level (H o = 0.351) while Lao Cai was lowest (H o = 0.198). The degree of genetic differentiation among populations were high (G st = 0.301). Jaccard’s similarity coefficient values were from 0.021 to 0.910 with a mean of 0.302. A phenetic tree classified population into four main groups. Group one was Thua Thien Hue population with coefficients of genetic similarity approximately of 0.42 and group II contained population from Vinh Phuc, group III consisted of population from Thua Thien Hue, Vinh Phuc, Lao Cai, and Jilin, whereas group IV involved population from Thua Thien Hue and Jilin. Our study indicated that P. polyphylla genetic relationship and diversity levels were high.

Keywords: Genetic diversity; Paris polyphylla ; polymorphic bands; RAPD.

INTRODUCTION consequence this species is under vulnerable category. Thus, restoration and conservation of the Paris , a mostly self-pollination medicinal plant natural population of this potential herb is consists of approximately 24 species and is found prerequisites (Negi et al ., 2014). in the temperate zones and tropical regions of Europe and Asian continent. Several Paris species Since the introduction of RAPD markers in 1990 have been reported to be used as haemostatic and (Williams et al ., 1990), application in plant anti-inflammatory agents to treat traumatic genetic analysis has increased in an exponential injuries, snake bites, abscess, parotitis and mastitis manner. Molecular diversity studies in some (Deb et al ., 2015; Shah et al ., 2012; Wang et al ., threatened and rare using RAPD 2013). Currently, Paris polyphylla Smith is polymorphism and Shannon’s index have reported extensively studied in traditional systems of (Verma et al ., 2007). RAPD has been known with Indian, Chinese and Viet