RGD-Binding Integrins Revisited: How Recently Discovered Functions and Novel Synthetic Ligands (Re-)Shape an Ever-Evolving Field
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Duffy, and Mnss Group Systems
Blood Groups – Duffy, and MNSs Group Systems Qun Lu, MD Assistant Professor Division of Transfusion Medicine Department of Pathology and Laboratory Medicine UCLA, School of Medicine Los Angeles, California 2009-03-12 Duffy Blood Group System History . 1950: Mrs. Duffy, a multiply transfused hemophiliac woman, developed an antibody not reacting with the known RBC antigens. Corresponding antigen was named after Mrs. Duffy . 1951: Fyb antibody was described in a woman with 3 pregnancies. 1955: Majority of blacks tested Fy(a-b-) . 1975: Fy(a-b-) RBCs were shown to resist infection by malaria organism Plasmodium vivax. Later: more Duffy antigens (Fy3, Fy4, Fy5, Fy6) were discovered . ISBT: 008 for the Duffy Blood Group Duffy Antigens . Most common: Fya and Fyb. Present at 6 weeks of gestation, well developed at birth – anti- Fy can cause hemolytic disease of newborn . Duffy antigens can be destroyed by enzymes such as ficin, papain, bromelain, chymotrypsin, ZZAP . When compared to Rh or Kell antigens, Duffy antigens are not very immunogenic. So, anti-Fya or anti-Fyb is not common. Fy (a-b-) is not Fy null, but homozygous for Fyb gene, they express Fyb antigen in other tissues, but not on RBCs → only will produce anti-Fya, not anti-Fyb. Fy (a-b-) is negative for Fy6 antigen which is the receptor for P. vivax (Fy6 is + when Fya + or Fyb+) Duffy Antigens . Phenotype Frequencies Chinese Phenotype Whites % Blacks % % Fy (a+b-) 17 9 90.8 Fy (a+b+) 49 1 8.9 Fy (a-b+) 34 22 0.3 Fy (a-b-) rare 68 0 White donor population: Fya: 66% Caucasians, 10% Blacks, 99% Asians Fya – units: 35% Fyb: 83% Caucasians, 23% Blacks, 18.5% Asians Fyb – units: 15% Fy3: 100% Caucasians, 32% Blacks, 99.9% Asians Duffy Antigens . -
Human and Mouse CD Marker Handbook Human and Mouse CD Marker Key Markers - Human Key Markers - Mouse
Welcome to More Choice CD Marker Handbook For more information, please visit: Human bdbiosciences.com/eu/go/humancdmarkers Mouse bdbiosciences.com/eu/go/mousecdmarkers Human and Mouse CD Marker Handbook Human and Mouse CD Marker Key Markers - Human Key Markers - Mouse CD3 CD3 CD (cluster of differentiation) molecules are cell surface markers T Cell CD4 CD4 useful for the identification and characterization of leukocytes. The CD CD8 CD8 nomenclature was developed and is maintained through the HLDA (Human Leukocyte Differentiation Antigens) workshop started in 1982. CD45R/B220 CD19 CD19 The goal is to provide standardization of monoclonal antibodies to B Cell CD20 CD22 (B cell activation marker) human antigens across laboratories. To characterize or “workshop” the antibodies, multiple laboratories carry out blind analyses of antibodies. These results independently validate antibody specificity. CD11c CD11c Dendritic Cell CD123 CD123 While the CD nomenclature has been developed for use with human antigens, it is applied to corresponding mouse antigens as well as antigens from other species. However, the mouse and other species NK Cell CD56 CD335 (NKp46) antibodies are not tested by HLDA. Human CD markers were reviewed by the HLDA. New CD markers Stem Cell/ CD34 CD34 were established at the HLDA9 meeting held in Barcelona in 2010. For Precursor hematopoetic stem cell only hematopoetic stem cell only additional information and CD markers please visit www.hcdm.org. Macrophage/ CD14 CD11b/ Mac-1 Monocyte CD33 Ly-71 (F4/80) CD66b Granulocyte CD66b Gr-1/Ly6G Ly6C CD41 CD41 CD61 (Integrin b3) CD61 Platelet CD9 CD62 CD62P (activated platelets) CD235a CD235a Erythrocyte Ter-119 CD146 MECA-32 CD106 CD146 Endothelial Cell CD31 CD62E (activated endothelial cells) Epithelial Cell CD236 CD326 (EPCAM1) For Research Use Only. -
Mixed Signals Nicholas Puckett
The Oval Volume 11 | Issue 1 Article 19 4-15-2018 Mixed Signals Nicholas Puckett Let us know how access to this document benefits ouy . Follow this and additional works at: https://scholarworks.umt.edu/oval Recommended Citation Puckett, Nicholas (2018) "Mixed Signals," The Oval: Vol. 11 : Iss. 1 , Article 19. Available at: https://scholarworks.umt.edu/oval/vol11/iss1/19 This Prose is brought to you for free and open access by ScholarWorks at University of Montana. It has been accepted for inclusion in The Oval by an authorized editor of ScholarWorks at University of Montana. For more information, please contact [email protected]. FICTION MIXED SIGNALS Nicholas Puckett No other pitcher in the minor leagues had a more unfortu- nate name than Homer Walker. It was Homer’s adopted father who named him after himself, and his father, and his father before him. The name was planned, as was Mrs. Walker’s pregnancy, but after numerous failed attempts at getting pregnant, which eventually left Mr. Walker finally conceding that it was, indeed, “his fault,” and numerous rejections from numerous adoption centers, they settled on the blonde-haired boy whom they named Homer. He was used to fans and teammates making fun of him for having such a perfectly ridiculous name, but their chiding was si- lenced once they saw him pitch. He could throw a fastball a hun- dred miles per hour and had a curveball that spun batters into the dirt like a corkscrew when they swung. Those were his high school days, at least. Then, he looked entirely different. -
Roots101art & Writing Activities
Art & Writing Roots101Activities ART & WRITING ACTIVITIES 1 Roots-inspired mural by AIC students Speaker Box by Waring Elementary student "Annie's Tomorrow" by Annie Seng TABLE OF CONTENTS Introduction 2 Roots Trading Cards 3 Warm-ups 6 Activity #1: Also Known As 12 Activity #2: Phrenology Portrait 14 Activity #3: Album Cover Design 16 Activity #4: Musical Invention 18 Activity #5: Speaker Boxes 20 Activity #6: Self-Portrait on Vinyl 22 ART & WRITING ACTIVITIES 1 Through The Roots Mural Project, Mural Arts honors INTRODUCTION homegrown hip hop trailblazers, cultural icons, and GRAMMY® Award winners, The Roots. From founders Tariq “Black Thought” Trotter’s and Ahmir “?uestlove” Thompson’s humble beginnings at the Philadelphia High School for Creative and Performing Arts (CAPA), to their double digit recorded albums and EPs, to an endless overseas touring schedule, and their current position as house band on “Late Night with Jimmy Fallon” on NBC, The Roots have influenced generations of artists locally, nationally, and globally. As Mural Arts continues to redefine muralism in the 21st century, this project was one of its most ambitious and far-reaching yet, including elements such as audio and video, city-wide engagement through paint days, a talkback lecture series, and a pop-up studio. Working alongside SEFG Entertainment LLC and other arts and culture partners, Mural Arts produced a larger-than-life mural to showcase the breadth of The Roots’ musical accomplishments and their place in the pantheon of Philadelphia’s rich musical history. The mural is located at 512 S. Broad Street on the wall of World Communications Charter School, not far from CAPA, where The Roots were founded. -
PAPC Couples the Segmentation Clock to Somite Morphogenesis by Regulating N-Cadherin-Dependent Adhesion
© 2017. Published by The Company of Biologists Ltd | Development (2017) 144, 664-676 doi:10.1242/dev.143974 RESEARCH ARTICLE PAPC couples the segmentation clock to somite morphogenesis by regulating N-cadherin-dependent adhesion Jérome Chal1,2,3,4,5,*, Charlenè Guillot3,4,* and Olivier Pourquié1,2,3,4,5,6,7,‡ ABSTRACT specific level of the PSM called the determination front. The Vertebrate segmentation is characterized by the periodic formation of determination front is defined as a signaling threshold epithelial somites from the mesenchymal presomitic mesoderm implemented by posterior gradients of Wnt and FGF (Aulehla (PSM). How the rhythmic signaling pulse delivered by the et al., 2003; Diez del Corral and Storey, 2004; Dubrulle et al., segmentation clock is translated into the periodic morphogenesis of 2001; Hubaud and Pourquie, 2014; Sawada et al., 2001). Cells of somites remains poorly understood. Here, we focused on the role of the posterior PSM exhibit mesenchymal characteristics and paraxial protocadherin (PAPC/Pcdh8) in this process. We showed express Snail-related transcription factors (Dale et al., 2006; that in chicken and mouse embryos, PAPC expression is tightly Nieto, 2002). In the anterior PSM, cells downregulate snail/slug regulated by the clock and wavefront system in the posterior PSM. We expression and upregulate epithelialization-promoting factors such observed that PAPC exhibits a striking complementary pattern to N- as paraxis (Barnes et al., 1997; Sosic et al., 1997). This molecular cadherin (CDH2), marking the interface of the future somite boundary transition correlates with the anterior PSM cells progressively in the anterior PSM. Gain and loss of function of PAPC in chicken acquiring epithelial characteristics (Duband et al., 1987; Martins embryos disrupted somite segmentation by altering the CDH2- et al., 2009). -
PDF Download Mixed Signals Ebook
MIXED SIGNALS PDF, EPUB, EBOOK Liz Curtis Higgs | 384 pages | 04 May 2005 | Multnomah Press | 9781590524381 | English | Sisters, United States Mixed Signals PDF Book When I was relatively young and still inexperienced with girls, I blew up at a girl for "playing games" like this with me. He goes out of his way to do things for you but shies away from talking about his feelings. Oldest Newest Most Voted. You also need to think about your actions and how they will be perceived. For more stories like this, sign up for our newsletter! I think we all sort of know it when we see it, but taking a minute to really flesh this concept out is important. Sometimes, you spend weeks chatting up a girl before you go on a date. They commented on that apple-picking pic you just posted with a fire emoji? Guys like to be in charge of the chase sometimes, so if he's the one making the moves toward you, it's safe to say that he's interested in you. Thank you! It can take many different forms across many different cultures. Sometimes, wires get crossed, triggering vulnerabilities and insecurities that can throw you for a loop, but this advice from relationship pros can help you move forward from these common mixed signals. Or they DM about your stories but rarely respond when you DM to theirs. It does so by requiring you to listen to a number, letter, color, or other piece of information while looking at a set of numbers, symbols, letters, words, or other information. -
Standard Resolution 19MB
The e-journal of analog and digital sound. no.24 2009 NOTHING BUT ANALOG! MoMA’s New York Clearaudio, Lyra, Air Tight and More NEW Punk Exhibit Grateful Dead Book By Ben Fong-Torres STYLE: We Ride Ducati’s Newest Supermotard At Indy! Box Sets: AC/DC and Thelonius Monk Perreaux Returns to the US Bob Gendron Goes To NYC to View Punk’s Past at MoMA TONE A 1 NO.24 2 0 0 9 PUBLISHER Jeff Dorgay EDITOR Bob Golfen ART DIRECTOR Jean Dorgay r MUSIC EDITOR Ben Fong-Torres ASSISTANT Bob Gendron MUSIC EDITOR M USIC VISIONARY Terry Currier STYLE EDITOR Scott Tetzlaff C O N T R I B U T I N G Tom Caselli WRITERS Kurt Doslu Anne Farnsworth Joe Golfen Jesse Hamlin Rich Kent Ken Kessler Hood McTiernan Rick Moore Jerold O’Brien Michele Rundgren Todd Sageser Richard Simmons Jaan Uhelszki Randy Wells UBER CARTOONIST Liza Donnelly ADVERTISING Jeff Dorgay WEBSITE bloodymonster.com Cover Photo: Blondie, CBGB’s. 1977. Photograph by Godlis, Courtesy Museum of Modern Art Library tonepublications.com Editor Questions and Comments: [email protected] 800.432.4569 © 2009 Tone MAGAZIne, LLC All rights reserved. TONE A 2 NO.24 2 0 0 9 55 (on the cover) MoMA’s Punk Exhibit features Old School: 1 0 The Audio Research SP-9 By Kurt Doslu Journeyman Audiophile: 1 4 Moving Up The Cartridge Food Chain By Jeff Dorgay The Grateful Dead: 29 The Sound & The Songs By Ben Fong-Torres A BLE Home Is Where The TURNta 49 FOR Record Player Is EVERYONE By Jeff Dorgay Here Today, Gone Tomorrow: 55 MoMA’s New York Punk Exhibit By Bob Gendron Budget Gear: 89 How Much Analog Magic Can You Get for Under $100? By Jerold O’Brien by Ben Fong-Torres, published by Chronicle Books 7. -
Kinetic Properties of Collagen Receptors on Human Keratinocytes 2337
Journal of Cell Science 112, 2335-2345 (1999) 2335 Printed in Great Britain © The Company of Biologists Limited 1999 JCS9937 Integrin α and β subunit contribution to the kinetic properties of α2β1 collagen receptors on human keratinocytes analyzed under hydrodynamic conditions Bénédicte Masson-Gadais, Anne Pierres, Anne-Marie Benoliel, Pierre Bongrand* and Jean-Claude Lissitzky Laboratoire d’Immunologie, INSERM U 387, Hôpital de Sainte-Marguerite, BP 29, 13274 Marseille Cedex 09, France *Author for correspondence (e-mail: [email protected]) Accepted 10 May; published on WWW 24 June 1999 SUMMARY The adhesion of keratinocytes to type I collagen or with ligand recognition and also with the ligand-β1 chain laminin 5 was studied in a laminar flow chamber. These interactions responsible for bond stabilization. The latter experiments provided an insight into the binding kinetics hypothesis was supported by the finding that the partial of integrins in their natural environment and the effects of alteration of α2 chain function by inhibiting antibodies was monoclonal antibodies specific for α and β chains. Cells corrected by anti-β1 chain antibody TS2/16. These results driven by a force too low to alter the natural lifetime of a could not be ascribed to allosteric changes of the functional single bond displayed multiple arrests. Studying the region of β1 integrin subunits regulated by TS2/16 since frequency and duration of these arrests yielded fairly direct there was no competition between the binding of TS2/16 information on the rate of bond formation (on-rate) and and anti-α2 chain antibodies. dissociation (off-rate). Off-rate values obtained on collagen Interpreted within the framework of current concepts of or laminin 5 (0.06 seconds−1) were tenfold lower than values integrin-ligand binding topology, these data suggest that determined on selectins. -
Supplementary Table 1: Adhesion Genes Data Set
Supplementary Table 1: Adhesion genes data set PROBE Entrez Gene ID Celera Gene ID Gene_Symbol Gene_Name 160832 1 hCG201364.3 A1BG alpha-1-B glycoprotein 223658 1 hCG201364.3 A1BG alpha-1-B glycoprotein 212988 102 hCG40040.3 ADAM10 ADAM metallopeptidase domain 10 133411 4185 hCG28232.2 ADAM11 ADAM metallopeptidase domain 11 110695 8038 hCG40937.4 ADAM12 ADAM metallopeptidase domain 12 (meltrin alpha) 195222 8038 hCG40937.4 ADAM12 ADAM metallopeptidase domain 12 (meltrin alpha) 165344 8751 hCG20021.3 ADAM15 ADAM metallopeptidase domain 15 (metargidin) 189065 6868 null ADAM17 ADAM metallopeptidase domain 17 (tumor necrosis factor, alpha, converting enzyme) 108119 8728 hCG15398.4 ADAM19 ADAM metallopeptidase domain 19 (meltrin beta) 117763 8748 hCG20675.3 ADAM20 ADAM metallopeptidase domain 20 126448 8747 hCG1785634.2 ADAM21 ADAM metallopeptidase domain 21 208981 8747 hCG1785634.2|hCG2042897 ADAM21 ADAM metallopeptidase domain 21 180903 53616 hCG17212.4 ADAM22 ADAM metallopeptidase domain 22 177272 8745 hCG1811623.1 ADAM23 ADAM metallopeptidase domain 23 102384 10863 hCG1818505.1 ADAM28 ADAM metallopeptidase domain 28 119968 11086 hCG1786734.2 ADAM29 ADAM metallopeptidase domain 29 205542 11085 hCG1997196.1 ADAM30 ADAM metallopeptidase domain 30 148417 80332 hCG39255.4 ADAM33 ADAM metallopeptidase domain 33 140492 8756 hCG1789002.2 ADAM7 ADAM metallopeptidase domain 7 122603 101 hCG1816947.1 ADAM8 ADAM metallopeptidase domain 8 183965 8754 hCG1996391 ADAM9 ADAM metallopeptidase domain 9 (meltrin gamma) 129974 27299 hCG15447.3 ADAMDEC1 ADAM-like, -
Flow Reagents Single Color Antibodies CD Chart
CD CHART CD N° Alternative Name CD N° Alternative Name CD N° Alternative Name Beckman Coulter Clone Beckman Coulter Clone Beckman Coulter Clone T Cells B Cells Granulocytes NK Cells Macrophages/Monocytes Platelets Erythrocytes Stem Cells Dendritic Cells Endothelial Cells Epithelial Cells T Cells B Cells Granulocytes NK Cells Macrophages/Monocytes Platelets Erythrocytes Stem Cells Dendritic Cells Endothelial Cells Epithelial Cells T Cells B Cells Granulocytes NK Cells Macrophages/Monocytes Platelets Erythrocytes Stem Cells Dendritic Cells Endothelial Cells Epithelial Cells CD1a T6, R4, HTA1 Act p n n p n n S l CD99 MIC2 gene product, E2 p p p CD223 LAG-3 (Lymphocyte activation gene 3) Act n Act p n CD1b R1 Act p n n p n n S CD99R restricted CD99 p p CD224 GGT (γ-glutamyl transferase) p p p p p p CD1c R7, M241 Act S n n p n n S l CD100 SEMA4D (semaphorin 4D) p Low p p p n n CD225 Leu13, interferon induced transmembrane protein 1 (IFITM1). p p p p p CD1d R3 Act S n n Low n n S Intest CD101 V7, P126 Act n p n p n n p CD226 DNAM-1, PTA-1 Act n Act Act Act n p n CD1e R2 n n n n S CD102 ICAM-2 (intercellular adhesion molecule-2) p p n p Folli p CD227 MUC1, mucin 1, episialin, PUM, PEM, EMA, DF3, H23 Act p CD2 T11; Tp50; sheep red blood cell (SRBC) receptor; LFA-2 p S n p n n l CD103 HML-1 (human mucosal lymphocytes antigen 1), integrin aE chain S n n n n n n n l CD228 Melanotransferrin (MT), p97 p p CD3 T3, CD3 complex p n n n n n n n n n l CD104 integrin b4 chain; TSP-1180 n n n n n n n p p CD229 Ly9, T-lymphocyte surface antigen p p n p n -
Analysis of RNA Expression Profiles Identifies Dysregulated Vesicle Trafficking Pathways in Creutzfeldt-Jakob Disease
Molecular Neurobiology (2019) 56:5009–5024 https://doi.org/10.1007/s12035-018-1421-1 Analysis of RNA Expression Profiles Identifies Dysregulated Vesicle Trafficking Pathways in Creutzfeldt-Jakob Disease Anna Bartoletti-Stella1 & Patrizia Corrado2 & Nicola Mometto2 & Simone Baiardi2 & Pascal F. Durrenberger3 & Thomas Arzberger4,5 & Richard Reynolds6 & Hans Kretzschmar5 & Sabina Capellari1,2 & Piero Parchi1,7 Received: 18 July 2018 /Accepted: 1 November 2018 /Published online: 16 November 2018 # Springer Science+Business Media, LLC, part of Springer Nature 2018 Abstract Functional genomics applied to the study of RNA expression profiles identified several abnormal molecular processes in experimental prion disease. However, only a few similar studies have been carried out to date in a naturally occurring human prion disease. To better characterize the transcriptional cascades associated with sporadic Creutzfeldt-Jakob disease (sCJD), the most common human prion disease, we investigated the global gene expression profile in samples from the frontal cortex of 10 patients with sCJD and 10 non-neurological controls by microarray analysis. The comparison identified 333 highly differentially expressed genes (hDEGs) in sCJD. Functional enrichment Gene Ontology analysis revealed that hDEGs were mainly associated with synaptic transmission, including GABA (q value = 0.049) and glutamate (q value = 0.005) signaling, and the immune/ inflammatory response. Furthermore, the analysis of cellular components performed on hDEGs showed a compromised regu- lation of vesicle-mediated transport with mainly up-regulated genes related to the endosome (q value = 0.01), lysosome (q value = 0.04), and extracellular exosome (q value < 0.01). A targeted analysis of the retromer core component VPS35 (vacuolar protein sorting-associated protein 35) showed a down-regulation of gene expression (p value= 0.006) and reduced brain protein levels (p value= 0.002). -
Integrins: Roles in Cancer Development and As Treatment Targets
British Journal of Cancer (2004) 90, 561 – 565 & 2004 Cancer Research UK All rights reserved 0007 – 0920/04 $25.00 www.bjcancer.com Minireview Integrins: roles in cancer development and as treatment targets 1 ,1,2 H Jin and J Varner* 1John and Rebecca Moores Comprehensive Cancer Center, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0912, USA; 2Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0912, USA The integrin family of cell adhesion proteins promotes the attachment and migration of cells on the surrounding extracellular matrix (ECM). Through signals transduced upon integrin ligation by ECM proteins or immunoglobulin superfamily molecules, this family of proteins plays key roles in regulating tumour growth and metastasis as well as tumour angiogenesis. Several integrins play key roles in promoting tumour angiogenesis and tumour metastasis. Antagonists of several integrins (a5b1, avb3 and avb5) are now under evaluation in clinical trials to determine their potential as therapeutics for cancer and other diseases. British Journal of Cancer (2004) 90, 561 – 565. doi:10.1038/sj.bjc.6601576 www.bjcancer.com & 2004 Cancer Research UK Keywords: angiogenesis; metastasis; apoptosis; integrin a5b1; integrin avb3 During the last 10 years, novel insights into the mechanisms sequences (e.g., integrin a4b1 recognises EILDV and REDV in that regulate cell survival as well as cell migration and invasion alternatively spliced CS-1 fibronectin). Inhibitors of integrin have led to the development of novel integrin-based therapeutics function include function-blocking monoclonal antibodies, pep- for the treatment of cancer. Several integrins play important tide antagonists and small molecule peptide mimetics matrix roles in promoting cell proliferation, migration and survival (reviewed in Hynes, 1992; Cheresh, 1993).