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Buffalo Bulletin (September 2014) Vol.33 No.3 general health condition, retarded growth, lower and eastern part consists of the Nara Desert. The output of work, and decrease in the production study area lies in the sub-tropical region between of milk and meat. Moreover, fi nancial losses are 27°31’48”N and 68°44’23”E. District Khaipur also signifi cantly associated with nagging irritation has eight sub-divisions (Talukas): Khairpur and depreciation of the value of skins and hides city, Kingri, Gambat, Sobhodero, Kotdeji, Nara, (Chaudhry et al., 2011). Mirwah and Faizgunj. This cross-sectional Pakistan being a tropical and sub-tropical epidemiological survey was conducted at region country, optimal climatic conditions different locations of the district to analyze the are very conducive for raising the buffaloes variation in tick infestation during 2008 and 2009. and these conditions are also favorable for the growth and development of many tick species Sampling of animals (Durrani and Shakoori, 2009). So under these Random sampling was used to select a conditions crossbred cows are considered total of 1600 buffaloes from the study area of unfit for survivability and adaptability. Poor Khairpur District. The samples were collected managemental practices further enhance the severity from small dairy herds having no history of use of the problem (Irshad et al., 2010). Under these poor of acaricide for tick control. About 107 farms and conditions the buffalo is the only animal that survives 1600 animals were selected for sampling of ticks. with optimal production. The buffalo is also prone Animals were categorized on the basis of breed to tick infestation but its adaptability in tropical and (Kundi vs. Nili-Ravi buffaloes) and age (< 1 year sub-tropical conditions is excellent. Previous fi ndings vs. 1-2 years vs. > 2 years). of tick prevalence report that exotic breeds suffer more as compared to local breeds of cattle and Sampling and morphological identifi cation of buffaloes in the climatic conditions of Pakistan.. ticks With regard to the tick prevalence in buffaloes Ticks were collected from body parts there is scanty information available concerning of the infested animals and careful sampling Pakistan (Manan et al., 2007; Sajid et al., 2008, was made using forceps to avoid de-capitulation 2009) and there is no report is available on tick (Solusby, 2006). After collection ticks were kept prevalence from the area of Sindh. Therefore, the in capped bottles properly labeled containing present cross-sectional study was focused on 70% ethyl alcohol for preservation. Morphological prevalence and identifi cation of genera of ticks identifi cation of the ticks was made in a laboratory infesting the buffaloes in the upper areas of Sindh at the Department of Veterinary Parasitology, Sindh province, Pakistan. Agriculture University Tandojam (Soulsby, 1982; Walker et al., 2007). MATERIALS AND METHODS Statistical analyses Statistical analyses were carried out by The study area included the district of using statistical package for social science (SPSS) Khairpur Mirs of the upper Sindh (Pakistan). It version 11.5. Multiple logistic regression tests were is situated south of the Indus River in the Sindh performed to determine the association between 324.