Are Folates, Carotenoids and Vitamin C Affected by Cooking?
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Are folates, carotenoids and vitamin C affected by cooking? Four domestic procedures are compared on a large diversity of frozen vegetables Sylvie Bureau, Sonia Mouhoubi, Line Touloumet, Caroline Garcia, Florie Moreau, Valérie Bédouet, Catherine Renard To cite this version: Sylvie Bureau, Sonia Mouhoubi, Line Touloumet, Caroline Garcia, Florie Moreau, et al.. Are folates, carotenoids and vitamin C affected by cooking? Four domestic procedures are compared on alarge diversity of frozen vegetables. LWT - Food Science and Technology, Elsevier, 2015, 64 (2), pp.735-741. 10.1016/j.lwt.2015.06.016. hal-02635963 HAL Id: hal-02635963 https://hal.inrae.fr/hal-02635963 Submitted on 27 May 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Version définitive du manuscrit publiée dans / Final version of the manuscript published in : LWT – Food Science and Technology (2015), Vol. 64, p. 735-741, DOI: 10.1016/j.lwt.2015.06.016 Journal homepage : http://www.elsevier.com/locate/lwt ipt cr nus a m Are folates, carotenoids and vitamin C affected by cooking? Four domestic procedures are compared on a large diversity of frozen / Author vegetables r u e * Sylvie Bureau a, b, , Sonia Mouhoubi a, b, Line Touloumet a, b, Caroline Garcia a, b, ut c c a, b ’a Florie Moreau , Valerie Bedouet , Catherine M.G.C. Renard a it d INRA, UMR408 Securite et Qualite des Produits d'Origine Vegetale, F-84000 Avignon, France b cr Universite d'Avignon et des Pays de Vaucluse, UMR408 Securite et Qualite des Produits d'Origine Vegetale, F-84000 Avignon, France c Toupargel, 13, chemin des pres secs, 69380 Civrieux d'Azergues, France nus a M article info abstract Article history: Four home procedures such as boiling in water, steaming, pressure steaming and microwave cooking Received 21 April 2015 were tested on 13 frozen vegetables. Folates, carotenoids and vitamin C were characterized on uncooked Received in revised form and cooked vegetables and illustrated a very large variability among the studied vegetables. The effect of ipt 5 June 2015 cooking was significant but it varied according to vegetables and phytochemicals. The best method to cr Accepted 9 June 2015 preserve the nutritional quality could be alternatively steaming, microwaving or pressure cooking, Available online 25 June 2015 nus whereas boiling was generally the less suitable method. On the fresh weight basis, boiling involved a a high loss of total vitamin C (average of À51% on fresh matter) and folates (À68%) and a slight loss of m Keywords: À À Domestic cooking lutein ( 15%) and b-carotene ( 9%). On the dry weight basis, it remained the less suitable for vitamin C À À þ Reprocessing ( 44%) and folates ( 65%) but not for carotenoids, as it allowed a better extractability of lutein ( 9%) and Nutritional quality b-carotene (þ20%). Version postprint Vegetables © 2015 Elsevier Ltd. All rights reserved. / Author Ascorbate r u e ut ’a it d 1. Introduction (vitamins) and has been already studied on vegetables (Bernhardt cr & Schlich, 2006; Gebczynski & Lisiewska, 2006; Mazzeo et al., Vegetables are a class of plant foods that are eaten as fresh, 2011; Miglio, Chiavaro, Visconti, Fogliano, & Pellegrini, 2008; nus a canned, frozen and cooked vegetables. Beyond its effect on texture Pellegrini et al., 2010; Sultana, Anwar, & Iqbal, 2008; Turkmen, M and taste, cooking also changes the nutritional properties of veg- Sari, & Velioglu, 2005; Volden, Borge, Hansen, Wicklund, & etables. Main phytochemicals such as vitamin C, folates (vitamin Bengtsson, 2009). Different procedures have been tested such as B9) and provitamin A carotenoids (b, a and g-carotene and b- boiling, microwave cooking, steaming, pressure steaming, frying cryptoxanthin) are present in vegetables. Among non-vitaminic and stewing. Their effects have been observed on different families compounds, lutein (Calvo, 2005) and lycopene (Singh & Goyal, of phytochemicals i.e. total polyphenols, carotenoids, a-tocopherol, ipt 2008) are also mostly provided in the diet by green leafy vegeta- glucosinolates, vitamin C, anthocyanins and chlorophylls. A large cr bles and tomato, respectively. Lutein plays a major role against diversity of vegetables has been concerned by these researches and macula degeneration (Granado, Olmedilla, & Blanco, 2003), while it is encouraged to consume a large diversity of them to consume all nus a lycopene has proven effects in prevention of prostate cancer nutrients and with a practical point of view to express the data on a m (Bramley, 2000). dry weight basis to allow a good comparison taking into account The effect of domestic cooking on phytochemicals (carotenoids the moisture change (Rickman et al., 2007a and b). non-vitamin C, polyphenols, chlorophylls) and on micronutrients However data are fragmented because studies concern rarely all phytochemicals, usually on one or two vegetable species, and / Author testing one or two of cooking methods, which makes difficult to ur have a good view of the effect of home cooking on the nutritional e * Corresponding author. INRA, UMR408 Securit e et Qualite des Produits d'Origine ut quality of vegetables. In addition, most of these studies have been Veg etale, Centre de Recherche PACA, 228 route de l'Aerodrome, CS 40509, Domaine ’a Saint Paul e Site Agroparc, 84914 Avignon Cedex 9, France. focused on fresh vegetables, while modern lifestyles lead con- E-mail address: [email protected] (S. Bureau). sumers to resort increasingly to processed vegetables (primarily it d cr http://dx.doi.org/10.1016/j.lwt.2015.06.016 © nus 0023-6438/ 2015 Elsevier Ltd. All rights reserved. a M Comment citer ce document : Bureau, S. (Auteur de correspondance), Mouhoubi, S., Touloumet, L., Garcia, C., Moreau, F., Bédouet, V., Renard, C. (2015). Are folates, carotenoids and vitamin C affected by cooking? Four domestic procedures are compared on a large diversity of frozen vegetables. LWT - Food Science and Technology, 64 (2), 735-741. DOI : 10.1016/j.lwt.2015.06.016 Version définitive du manuscrit publiée dans / Final version of the manuscript published in : LWT – Food Science and Technology (2015), Vol. 64, p. 735-741, DOI: 10.1016/j.lwt.2015.06.016 736Journal homepage : http://www.elsevier.com/locateS. Bureau et al. / LWT - Food/lwt Science and Technology 64 (2015) 735e741 canned and frozen). Frozen vegetables are convenient and, after the the water during cooking. The time of cooking given in Table 1 original reduction caused by blanching and freezing, maintain corresponded to the time after the first issue of vapor. Just after nutritional qualities levels at À20 C during storage for water sol- cooking, vegetables were drained, chilled in an ice water bath uble antioxidant activity in peas and spinach (Hunter & Fletcher, during five minutes, drained, stabilized by freezing in liquid ni- 2002). However, our recent studies (Delchier, Reich, & Renard, trogen and stored at À80 C until analysis. 2012) indicated that effects of cooking were exacerbated on frozen vegetables, mainly due to facilitated losses by leaching. 2.2.3. Steaming (ST) ipt The aim of this work was to understand how an industrial As no recommendation was indicated on packaging, the cooking cr processing (blanching and freezing) interacts with domestic cook- time was defined experimentally and collectively by 6 persons of nus ing practices to impact the nutritional content of vegetables. the laboratory who tasted each vegetable after different cooking a Indeed, the effect of four common home cooking procedures, times. Each portion of frozen vegetables was steamed according to m boiling in water, microwave cooking, steaming and pressure this defined time (Table 1). 1 L of water was used to generate the ® steaming, was studied on the micronutrient content of blanched steam. Steaming was carried out using SEB “Vitacuisine Compact ” and frozen vegetables. The representative micronutrients were (SEB, Selongey, France). At the end of steaming, vegetables were hydrosoluble compounds such as vitamin C and folates, and phy- directly stabilized by freezing in liquid nitrogen and stored / Author r tochemicals such as carotenoids for lipidic compounds. As different at À80 C until analysis. u & e effects were highlighted depending on the vegetables (Danesi ut Bordoni, 2008), thirteen blanched and frozen vegetables, repre- 2.2.4. Microwave cooking (MW) ’a sentative of a large diversity in composition and in shape, were In the microwave (Whirlpool, Family chef) no additional water it d characterized before and after cooking procedures. was used. The vegetables were placed in a dish with a lid and were cr stirred at half of the cooking time (Table 1). Heating occurred with a 2. Materials and methods power of 750 W, i.e. 1.5 W/g. Vegetables were then stabilized by nus a freezing in liquid nitrogen and stored at À80 C until analysis. M 2.1. Materials 2.3. Samples preparation Thirteen vegetables were provided by a French company specialized in home delivery of frozen products (Toupargel, Civ- Before analysis, the frozen samples were ground in liquid ni- rieux d'Azergues). During their processing, vegetables were sys- trogen to obtain a homogenised powder which was stored À À ipt tematically blanched before freezing and then stored at 20 C. The at 80 C. In parallel to the cooking tests, three sets of 500 g of each cr studied vegetables were: (1) green and (2) yellow French bean uncooked vegetable, blanched and frozen, were directly taken in (Phaseolus vulgaris L.), (3) pea (Pisum sativum L.), (4) Brussels sprout the marketed bags.