Polyclonal Antibody to GAD1 / GAD67 - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP31805PU-N Polyclonal Antibody to GAD1 / GAD67 - Aff - Purified

Alternate names: 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 1, Glutamate decarboxylase 67 kDa isoform Quantity: 0.1 mg Concentration: 0.1 mg/ml (based on Bradford assay readings using BSA as a standard). Background: Human Glutamic Acid Decarboxylase (GAD-67), [EC 4.1.1.15] is a 66,987 dalton protein (594 amino acids) selectively expressed in a subpopulation of GABAergic neurons of the CNS. It catalyzes the decarboxylation of glutamic acid, forming the inhibitory neurotransmitter β-amino butyric acid (GABA). It is also know as GAD-1. Uniprot ID: Q99259 NCBI: NP_000808 GeneID: 2571 Host / Isotype: Chicken / Ig Immunogen: Synthetic peptide KLH conjugated corresponding to a region near the C-terminus of this gene product, and was 100% conserved between the Human (Q99259), Mouse (P48318) and Rat (NP_058703) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column. Format: State: Liquid purified (0.45 µm filter sterilized) IgY fraction. Purification: Affinity Chromatography using a peptide column. Buffer System: 10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative. Applications: Western Blot. Immunocytochemistry. Immunohistochemistry. Recommended Dilutions: 1/2000-1/5000 for Western blots. 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Quality Control: This antibody was analyzed by Immunohistochemistry (at a concentration of 1 µg/ml) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: Recognizes Glutamic Acid Decarboxylase (GAD1/GAD67). Species: Human, Mouse, Rat Other species not tested. Storage: Store the antibody undiluted in the dark at 2-8°C. Shelf life: one year from despatch.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. RS/20150305 1 / 2 AP31805PU-N: Polyclonal Antibody to GAD1 / GAD67 - Aff - Purified

Pictures: A tissue section through an adult Mouse brain showing GAD-1 (red staining) in basket cells of the hippocampal formation. Green staining is autofluorescence from green fluorescent protein (GFP) expressed in this transgenic Mouse. Picture courtesy of Dr. Felix Eckenstein, University of Vermont.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. RS/20150305 2 / 2