Polyclonal Antibody to GAD1 / GAD67 (1-100) - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP08435PU-N Polyclonal Antibody to GAD1 / GAD67 (1-100) - Aff - Purified

Alternate names: 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 1, Glutamate decarboxylase 67 kDa isoform Quantity: 50 µg Concentration: 0.5 mg/ml Background: GAD1/GAD67, a 67KDa protein, is a neuronal isoform of this enzyme predominantly expressed in human Brain. It consists of Pyridoxal 5-phosphate binding site and conserved Cysteine residues which are vital for its activity and is inhibited due to phosphorylation. It has high binding affinity for the cofactor Pyridoxal-5phosphate and its role has been implicated in normal Palate development. It is found to be associated with childhood onset schizophrenia and craniofacial malformations and has been identified as a major auto antigen and an auto-reactive T-cell target in IDDM. Deficiency of this enzyme leads to pyridoxine dependency along with epilepsy. It also might play a role in stiff main syndrome. Uniprot ID: Q99259 NCBI: NP_000808.2 GeneID: 2571 Host / Isotype: Rabbit / IgG Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids 1-100 of human GAD67 Remarks: The sequence used is 100% homologous with mouse and 94% homologous with rat GAD67. Format: State: Liquid purified Ig fraction Purification: Protein G Chromatography Buffer System: PBS containing 0.2% Gelatin as stabilizer and 0.05% Sodium Azide as preservative Applications: Immunohistochemistry on Paraffin Sections: 10 µg/ml. Western Blot: 5 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody recognizes Glutamate Decarboxylase 1 (GAD1/GAD67) at aa 1-100. Species Reactivity: Tested: Human, Mouse and Rat Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20170919 1 / 2 AP08435PU-N: Polyclonal Antibody to GAD1 / GAD67 (1-100) - Aff - Purified

Pictures: Testis: Formalin-Fixed Paraffin- Embedded (FFPE)

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20170919 2 / 2